You are conducting a CRISPR-Cas9 experiment. You transform a cell with Cas9 and a guide RNA (contains the scaffold and guide sequences), but then realize there is no PAM sequence near your target sequence. What is the likely outcome of this

Answers

Answer 1

Answer:

Cas9-sgRNA cannot align with targeted DNA sequence

Explanation:

Without a PAM sequence, the CRISPR-sgRNA (small guide RNA) complex cannot align with the targeted DNA sequence. Therefore, you cannot perform experiments (e.g. gene editing) using CRISPR-Cas9.


Related Questions

A__ forms when a volcano collapses in on itself.

Answers

Answer:

A caldera

Explanation:

This forms when a volcano collapses in on itself.

Have a nice day and hope this helps.

Answer:

A caldera forms

Explanation:

(50 POINTS) Which of the following could be alive because it displays one of the signs of life? An object that _____.

A. has branches
B. does not move when touched
C. moves away from loud noises
D. makes noise

Answers

Answer:

A.

Explanation:

An object with branches

During replication an exact copy of a DNA ____________ is produced.

Answers

Answer:

The replication process

I Using the key choices, identify the cell type(s) or blood elements that fit the following descriptions. Insert the correct term or letter response in the spaces provided. Key Choices A. Red blood cell D. Basophil G. Lymphocyte B. Megakaryocyte E. Monocyte H. Formed elements C. Eosinophil F. Neutrophil I. Plasma _________________________ 1. Most numerous leukocyte _________________________ 2. ___________________ 3. ___________________ 4. Granular leukocytes _________________________ 5. Also called an erythrocyte; anucleate _________________________ 6. _________________________ 7. Actively phagocytic leukocytes _________________________ 8. _________________________ 9. Agranular leukocytes _________________________10. Fragments to form platelets _________________________ 11. (A) through (G) are examples of these _________________________12. Increases during parasite attacks _________________________13. Releases histamine during inflammatory reactions _________________________14. After originating in bone marrow, may be formed in lymphoid tissue _________________________15. Contains hemoglobin _________________________16. Primarily water, noncellular; the fluid matrix of blood _________________________17. Increases in number during prolonged infections _________________________18. Least numerous leukocyte

Answers

Answer:

NeutrophilNeutrophilEosinophilBasophilRed blood cellMonocyteNeutrophilMonocyte LymphocyteMegakaryocyteFormed elementsEosinophilBasophilLymphocyteRed blood cellPlasmaMonocyteBasophil

Explanation:

A. Red blood cell: Blood cell that is a product of the medulla. These cells contain hemoglobin that carries oxygen from the lungs to the rest of the body.

B. Megakaryocyte:  Big cell easy to recognize for its size. Differs from other cells for being polyploid, growing by endomitosis, and being bigger. They have important biological functions. The most important one is the formation of plaquettes, necessary for hemostasia, damaged vessels reparation, and tissue healing.

C. Eosinophil: Granulosites. Immune System cells, active during infections, pathologies, inflammations, and allergies.  Originated in the medulla and transported to the blood and organs. One of their functions is to defend the organisms from parasite infections, helping to destroy the pathogen.

D. Basophil: Granulosites. Immune System cells.  in the medulla and transported to the blood. Only when there are parasite infections they can be present in tissues and organs. They release substances such as histamine that make possible the inflammatory process.

E. Monocyte: Agranulocyte phagocyte. Originated in the medulla and transported to the rest of the body. They differentiate into macrophages in tissues. They defend the body from bacteria and viruses. When monocyte values are elevated, usually means that there is an important process of infection.

F. Neutrophil: Phagocytic Leucocytes polymorphonuclear found in the peripheric blood. Compose the first defense line against bacterial and fungal infections.  Product of the medulla and transported to the blood. They are the first to arrive at the affected tissue, followed by lymphocytes and macrophages.    

G. Lymphocyte: Leucocyte. There are two types of lymphocytes: B cells, which can produce antibodies in the presence of bacteria, viruses, and toxins. T cells can destroy infected cells from the body. They all circulate in the blood.

H. Formed elements: Elements that might be found suspended in the plasma. These include all the mentioned cells and cell fragments      

I. Plasma: Transparent, acellular, and kind of yellow liquid that represents 55% of the blood volume.            

The function of platelets is all of the following except:

A.they help form blood clots

B.they help a blood vessel repair itself •

C. they carry oxygen to injured areas of tissue

D. they secrete vasoconstrictors which promote vascular spasms

Answers

Answer:

C

Explanation:

They does not carry oxygen to injured areas of tissue

Is a human egg cell a somatic cell or a gamete?

Answers

Answer:

A human egg cell would be a gamete cell, not a somatic cell

Explanation:

explain the concept of linkage. if you performed a test cross with a fruit fly that is heterozygous for two genes how could you conclude that the two genes are linked

Answers

Answer:

The correct answer would be - the ratio of phenotypes in an organism cross will tell if the genes are linked or not.

Explanation:

In this question, we perform a test cross with a fruit fly that is heterozygous If we perform a test cross between two parents with identical heterozygous genomes AaBb.

If there is no recombination found in the offspring of this cross it means there are no linkage of genes present.  And if there are new or recombinant phenotypes present it means that there are genes linked together.

6. What does eutrophication cause?
O A. A reduction in water pollution
O B. Increases in fish populations
O C. A new food source for birds
D. Toxic algae blooms

Answers

Answer:

d toxic algae blooms

Explanation:

because it is eutrophication

Decline of pollinators will have a severe impact on nature and mankind. why?
please answer and I will mark you as brainlist. ​

Answers

Answer:

Globally we are witnessing a decline in pollinators, such as wild bees, honeybees and hover flies, caused by the destruction and fragmentation of habitats, agricultural intensification and use of pesticides, introduction of novel diseases and competing alien species, and climate change

Summary how the fast carbon cycle works?

Answers

Answer:

here is your answer

Explanation:

Any change in the cycle that shifts carbon out of one reservoir puts more carbon in the other reservoirs. Changes that put carbon gases into the atmosphere result in warmer temperatures on Earth. This diagram of the fast carbon cycle shows the movement of carbon between land, atmosphere, and oceans.

What the answer to this

Answers

this answer to this question is true

The diagram compares diffusion of sugar molecules and osmosis. Explain the difference between osmosis and diffusion in cells

Answers

Answer:

What is the difference between osmosis and diffusion? Osmosis is the movement of water molecules through the cell. Diffusion is the movement of molecules, such as oxygen, in and out of a cell. The process by which water molecules are able to diffuse through the cell membrane.

Explanation:

Diffusion and osmosis involves the movement of materials from one region another. The main difference between osmosis and diffusion is that osmosis requires a semi permeable membrane.

The movement of materials always occur in cells. This movement could occur by;

Active transportOsmosisdiffusion

The difference between osmosis and diffusion is that, diffusion does not involve a semi permeable membrane.

A semi permeable membrane is a membrane that is selectively permeable to materials. It allows some materials to pass and does not allow other materials to pass. We can see the semi permeable membrane as the structure in the osmosis diagram that separates the two regions.

Diffusion does not require the use of a semi permeable membrane.

Learn more:https://brainly.com/question/19867503

Why would looking for the persons responsible for sneaking endangered species (rare birds, for example) into the United States be considered a job for a forensic scientist

Answers

Answer:

Forensic scientists are able to find the answer.

Explanation:

The persons responsible for sneaking endangered species into the United States be considered a job for a forensic scientist because forensic scientist is able to find out the persons. Forensic scientist are the investigators who collect evidences from the crime scene and leads to the person who commit crime. So the forensic scientist is the only person who find out the persons responsible for sneaking endangered species.

Through the process of natural selection, favorable forms of genes are passed on more than other forms of genes.

A. True

B. False

Answers

true because when you have a gene it replicates.
true i thinkkkkkkkkkk

which among the 7 theories on the origin of life would you
believed as true?​

Answers

Answer:

Life on Earth began more than 3 billion years ago, evolving from the most basic of microbes into a dazzling array of complexity over time. But how did the first organisms on the only known home to life in the universe develop from the primordial soup?

One theory involved a "shocking" start. Another idea is utterly chilling. And one theory is out of this world!

Inside you'll learn just how mysterious this all is, as we reveal the different scientific theories on the origins of life on Earth. Electric sparks can generate amino acids and sugars from an atmosphere loaded with water, methane, ammonia and hydrogen, as was shown in the famous Miller-Urey experiment reported in 1953, suggesting that lightning might have helped create the key building blocks of life on Earth in its early days. Over millions of years, larger and more complex molecules could form. Although research since then has revealed the early atmosphere of Earth was actually hydrogen-poor, scientists have suggested that volcanic clouds in the early atmosphere might have held methane, ammonia and hydrogen and been filled with lightning as well.

The deep-sea vent theory suggests that life may have begun at submarine hydrothermal vents spewing key hydrogen-rich molecules. Their rocky nooks could then have concentrated these molecules together and provided mineral catalysts for critical reactions. Even now, these vents, rich in chemical and thermal energy, sustain vibrant ecosystems.

The next idea is a chilling thought. Read on!

Ice might have covered the oceans 3 billion years ago, as the sun was about a third less luminous than it is now, scientists say. This layer of ice, possibly hundreds of feet thick, might have protected fragile organic compounds in the water below from ultraviolet light and destruction from cosmic impacts. The cold might have also helped these molecules to survive longer, allowing key reactions to happen. [Related: The Ingredients of Life]

Understanding life’s origin may involve unravelling the mystery of DNA's formation, as we explain next.Nowadays DNA needs proteins in order to form, and proteins require DNA to form, so how could these have formed without each other? The answer may be RNA, which can store information like DNA, serve as an enzyme like proteins, and help create both DNA and proteins. Later DNA and proteins succeeded this "RNA world," because they are more efficient.

RNA still exists and performs several functions in organisms, including acting as an on-off switch for some genes. The question still remains how RNA got here in the first place. And while some scientists think the molecule could have spontaneously arisen on Earth, others say that was very unlikely to have happened. Other nucleic acids other than RNA have been suggested as well, such as the more esoteric PNA or TNA.

A study in 2015 suggests the missing link in this RNA puzzle may have been found.

Instead of developing from complex molecules such as RNA, life might have begun with smaller molecules interacting with each other in cycles of reactions. These might have been contained in simple capsules akin to cell membranes, and over time more complex molecules that performed these reactions better than the smaller ones could have evolved, scenarios dubbed "metabolism-first" models, as opposed to the "gene-first" model of the "RNA world" hypothesis.

The final theory is truly out of this world. Perhaps life did not begin on Earth at all, but was brought here from elsewhere in space, a notion known as panspermia. For instance, rocks regularly get blasted off Mars by cosmic impacts, and a number of Martian meteorites have been found on Earth that some researchers have controversially suggested brought microbes over here, potentially making us all Martians originally. Other scientists have even suggested that life might have hitchhiked on comets from other star systems. However, even if this concept were true, the question of how life began on Earth would then only change to how life began elsewhere in space.

Oh, and if you thought all that was mysterious, consider this: Scientists admit they don't even have a good definition of life!

If you used a slingshot to launch a bowling ball and a baseball with the same force, which would go further? Explain your reasoning.​

Answers

Answer: The baseball will go farther because of Newton's law. The more mass an object as, the more force needed to move it. The bowling ball has more mass than a baseball so more force is needed to move it.

Explanation:

Help plzzzzzz I need helppp

Answers

where are you traveling from and to?

Oxygen crosses the cell membrane by

Answers

Answer:

diffusion

Water, carbon dioxide, and oxygen are among the few simple molecules that can cross the cell membrane by diffusion (or a type of diffusion known as osmosis ).

Explanation:

The map shows the location of Earth's principal biomes.

Which abiotic factor in tropical forests do boreal forests lack?

Answers

Answer:

warm temperature

Explanation:

I cannot see the choices in your image. I can only see choice A, which is "soil."

"Abiotic factors" are part of the ecosystem that don't have life. However, they have an impact on the life of the biotic factors (living organisms) in the environment. Examples of abiotic factors are: sunlight, temperature, climate, soil, humidity, wind, etc.

"Boreal forests" are abundant on the Earth's Northern Hemisphere. The climate here is generally cold, which doesn't allow some trees to survive. It is totally opposite to the warm temperature and high humidity that you can feel in the "tropical forests." Such factors allow many plants and animals to survive.

Clearly, the boreal forests lack the "warm temperature" that the tropical forests have.

In which choices are the entries listed from largest to smallest
A: gene-cell-DNA-building block

B: chromosome-genome-DNA building block

C: genome-gene-nucleotide base

D:DNA building block-gene-chromosome-genome

Answers

C. A nucleotide base is the polymer of nucleic acids, such as DNA. A gene is the sequence of many nucleic acids and a genome is an organisms full set of genetic info.

Carriers have to be what
genotype?

Answers

Answer:

What is the genotype of a carrier? The genotype of the carrier is Ff (one dominant non-disease gene, F, and one recessive, CF gene, f). Of course, any letter of the alphabet could be used as long as it is expressed as a heterozygous genotype.

___________ It is a process in producing garden soil that will remove unwanted particles

to be a part of the garden soil. write your answers in small letters.​

Answers

Answer:

Adding organic matter or growing cover crops

Explanation:

Adding organic matter or growing cover crops  in the form of aged manure to the soil is a process used in producing garden soil and this is because not only will the compost make the land good and fertile it will also makes the garden soil friable unlike the application of fertilizer

Describe Ted Bundy’s childhood and his relationship with his family.

Answers

Answer:

Bundy was born at a home for unwed mothers in Burlington , Vermont, on November 24, 1946. He remained there for two months after his birth. His mother, Eleanor Louise Cowell, known as Louise, considered placing her baby up for adoption, but her father, Sam Cowell, apparently wanted the baby to join the family in Philadelphia. HOPE THIS HELPSSSSS!

Explanation:

Which of the following best matches a part of a living cell with the function it performs?

Cytoplasm: green structure inside plant cells
Nucleus: green structure inside plant cells
Cytoplasm: directs all of the cell's activities
Nucleus: directs all of the cell's activities

Answers

its the last optionnnn

Answer:

Nucleus: directs all of the cells activities

=~

Explanation:

I took the exam and its correct!! I take FLVS this is the 4.00 Pretest or 4.01 for science right? Any who I got it right so go get em babes!!

If I turn off all molecules expect for protein and amino acids what do I observe

Answers

Answer:

hidhyrydhrshrzhrshrsurshrshrzhrdhrzhrs

Answer: um first you need to show us a exmple

Explanation:

Jorge wants to model two processes from the water cycle in the atmosphere for a class project. He began to fill a self-sealed bag half full of water. After sealing it, he tapped it into the window. After a few hours, water beaded along the sides of the bag. Which processes are represented by Jorge's model?
A.transpiration and respiration
b.respiration and evaporation
c.evaporation and condensation
d. condensation and crystallization

Answers

C. Evaporation and Condensation

A sedentary organism is one that can swim great distances in aquatic environments

A. True

B. False

Answers

Answer: i believe it is true

Explanation:

I think it’s true, not a 100% sure though :)

What is actually cycled in a web of organisms?

Answers

Answer:

Carbondioxide gas.

Explanation:

Carbondioxide gas is actually cycled in a web of organisms, The plants takes carbondioxide gas for the process of photosynthesis, this plant is eaten by herbivores for energy and carbondioxide is released in the process of respiration. After that, carnivores feed on these herbivores and oxygen is used in the breakdown of food for releasing energy and carbondioxide gas is also released from the body through breathing so the cycle of carbondioxide gas is continue.

How many black Pups were produced? How many white pups were produced?

Answers

Answer:

In the lifetime of of two guinea pigs, 40 produce black pups and 40 white pups (black is the dominant trait).

Explanation:

Answer:

I don't really now what you are talking about

The unit discusses the problems with deforestation. What other problems could the extreme environmental damage caused by the kind of mining done in the Appalachians create?

I'm not sure if anyone can help me with this one, but if you can that'd be great! I have to write in the answer but I'm just looking for an idea. Thank you!

Answers

Answer:

Mining in the Appalachians has caused debris and harmful chemicals to land in the local waterways. The large amount of waste and dirt has also changed the course of the canals.

Explanation:

I took a majority of the information from a quiz prior to this assignment. I know that this is several days after you requested help but I hope that this helps you and other students!

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