If a researcher has a DNA sample with a concentration of 0.6 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 3.9 nanograms per microliter

Answers

Answer 1

Answer:

0.325 microliters

Explanation:

1 microgram = 1000 nanograms

DNA sample concentration= 1000 X 0.6 = 600 nanograms / microliters

We need a total of 50 microliter reactions, and the concentration should be 3.9 nanograms per microliter.

So, we need total 50 X 3.9 = 195 nanograms in 50 microliters.

Therefore, it required 195 / 600 = 0.325 microliters of DNA sample to be transferred.


Related Questions

Which factors affect the rate of photosynthesis select all that apply

Answers

Answer:

Limiting factors such as

Effect of temperature

Effect of light intensity

Concentration of carbon dioxide

These all factors can affect the rate of photosynthesis

Explanation:

I hope it will help you

Answer:

light intestity

rate of oxygen

carbon4oxide concentration

temperature

What are one benefit and one consequence of building a desalination plant? Would a desalination plant be beneficial to your local area? Explain why or why not.

Answers

Answer:

Desalination plants, also known as desalination plants, are industrial facilities for desalination, generally of the sea or salty lakes, to obtain drinking water.

Explanation:

The benefits are clear: having clean water in places where it is scarce. But, on the disadvantages side, it is a process that requires a lot of energy and is therefore expensive. In addition, desalination plants have an impact on marine ecosystems that must be assessed, they increase the temperature of sea water and reduce the amount of oxygen in the water, causing serious damage to aquatic life.

Answer:

a. Dams help make irrigation possible so that farming can occur in dry regions .

Explanation:

What do eukaryotic cells have that prokaryotic cells do not?

Answers

There are several differences between the two, but the biggest distinction between them is that eukaryotic cells have a distinct nucleus containing the cell's genetic material, while prokaryotic cells don't have a nucleus and have free-floating genetic material instead.

dependent and independent variable hi guys please help me i want an example of indepent and depent variable for ech variable example Note : please in science not in math in science Gratefully , Brainly Sthay home and keep safe

Answers

Answer:

The quantities between which a graph is plotted are called variables.The independent variables are those which do not depend on other quanitity for example time.The dependents variables are those which depend on other quantity for example velocity,acceleration,speed,momentum etc.

Answer:

It is B,D,E

(✿◠‿◠)

The process of cell division inveukaryotes is called?

Answers

Answer:

In eukaryotes, there are two distinct types of cell division: a vegetative division, whereby each daughter cell is genetically identical to the parent cell (mitosis), and a reproductive cell division, whereby the number of chromosomes in the daughter cells is reduced by half to produce haploid gametes (meiosis).

When two pink flowers ​(RW​) are​ crossed, there are four equally likely possible outcomes for the genetic makeup of the​ offspring: red ​(RR​), pink​(RW​), pink​(WR​), and white ​(WW​). If two pink snapdragons are​ crossed, what is the probability that the offspring will be​ (a) pink​, ​(b) red​, and​ (c) white​?

Answers

Answer:

a) 50% chance of being pink

b) 25% chance of being red

c) 25% change of being white

Explanation:

Since neither the R allele or W allele is dominant over the other, this is codominance. If 2 pink ones are crossed, that means both of them are RW

If we draw out the punnet square

     R      W

R   RR   RW

W  RW  WW

There is a 1:2:1 ratio of Red to Pink to White

Therefore

a) 50% chance of being pink

b) 25% chance of being red

c) 25% change of being white

Which of these actions can the Consumer Product Safety Commission take
to help protect consumers?
O A Force a store to issue store credit
O B. Mediate a disagreement between a consumer and a store
O C. Recall an unsafe product
O D. Make a company repait a damaged product​

Answers

Answer:

C. Recall an unsafe product

The Consumer Product Safety Commission (CPSC) is a U.S. government agency that is responsible for protecting consumers from unsafe products that are in Option C. The CPSC has the authority to take various actions to help protect consumers.

What is the Consumer Product Safety Commission?

The CPSC has the authority to recall any consumer product that poses a substantial risk of injury or death to consumers. The CPSC can require companies to recall their products, issue warnings to consumers, and take other appropriate actions to protect the public. Forcing a store to issue store credit is not a typical action that the CPSC would take to protect consumers. While the CPSC can investigate complaints and take legal action against companies that violate safety standards, it does not have the authority to force stores to issue store credit.

Hence, the Consumer Product Safety Commission (CPSC) is a U.S. government agency that is responsible for protecting consumers from unsafe products that are listed in Option C.

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Identify the correct statements about transcription(Tnx) and (Tnl) translation in prokaryotes and eukaryotes.
A. Prokaryotes conduct Tnx and Tnl together in the same cellular compartment.
B. Eukaryotes do Tnx in the nucleus and Tnl in the cytoplasm.
C. Tnx and Tnl occur together in the cytoplasm of Prokaryotes and Eukaryotes.
D. Tnx and Tnl both occur in the nucleus in eukaryotes and prokaryotes.

Answers

Answer:

A and B

Explanation:

The correct statements about transcription and translation would be that prokaryotes conduct transcription and translation together in the same cellular compartment and eukaryotes do transcription in the nucleus and translation in the cytoplasm.

Prokaryotes generally lack a nucleus and other membrane-bound organelles and as such, their genetic materials lie freely within the cytoplasm. Hence, transcription and translation take place within the same cellular compartment, which is the cytoplasm.

Eukaryotes, however, have a nucleus and other membrane-bound organelles such as mitochondrion. Their genetic materials lie majorly in the nucleus. Hence, transcription takes place within the nucleus. Thereafter, the transcribed mRNA is transported to the cytoplasm where they get attached to ribosome and tRNA and translated into proteins.

The correct options are A and B.

Which group of algae is the most closely related to vascular plant, what scientific evidence can be used to support your answer

Answers

Answer:

Explanation:

The green algae lineages hypothesized to be most closely related to land plants include: Coleochaetophyceae (coleochaetes). Charophyceae (stoneworts).well  lack  of vascular tissue—specialized groups of cells that conduct of  water or dissolved nutrients throughout the plants body.(i guess this the scientific evidenxe if not comment ill provide more information)

hope it helps

Which is one characteristic of producers

Answers

Answer:

They make their own food

Answer:

B.) they do not eat other organisms

Explanation:

name the most suitable apparatus for measuring exactly 1centimeter of hydrochloric acid​

Answers

Explanation:

measuring cylinder ig

Answer:

Measuring cylinder

Explanation:

Hydrochloric acid is in the form of liquid so to measure liquids (in liters), measuring cylinder is used.

If the Hemoccult test result is negative, no further testing is done. If the Hemoccult test result is positive, the individual will have a second stool sample tested with the Hemoccult II test. If this second sample also tests positive for blood, the individual will be referred for more extensive evaluation. What is the effect on net sensitivity and net specificity of this method of screening

Answers

Answer:

Hemoccult test sensitivity is 89.3% and specificity is 92.7%

Explanation:

Hemoccult test is a test that is carried out in order to find out the presence of occult blood in your stool. This method is used to detect colorectal cancer in your body. Blue color of the blood indicate that the test is positive while the green color represents that the test is negative. Test sensitivity is the ability of a test which identify the person who is affected with the disease i. e. true positive, whereas test specificity is the ability of the test to correctly identify the patient without the disease. The value of sensitivity of the Hemoccult test is 89.3% the specificity is 92.7%, and the positive predictive value is 2.5%.

Endotoxins Check All That Apply are released when a gram-negative cells lyses.are released when a gram-negative cells lyses. are synthesized and exported from the cell during the initial stages of infection.are synthesized and exported from the cell during the initial stages of infection. may cause septic shock.may cause septic shock. are small protein molecules.are small protein molecules.

Answers

Answer:

are released when a gram-negative cells lyses.

May cause septic shock.

are synthesized and exported from the cell during the initial stages of infection.

Explanation:

Endotoxins are liposacharides and toxic substances found in the outer membrane of gram negative bacteria. They are toxins produced within the cell of bacteria. These toxins are released when the bacteria ruptures or disintegrates. This can happen as a result of the immune system or antibiotics attack the bacteria and break down their cell walls. It causes inflammation and shock.

It can cause uneccessary proapoptotic in immune system during during sepsis resulting in damage to immune system which cause septic shock.

Statements that are right as regards Endotoxins are:

are released when a gram-negative cells lyses.

May cause septic shock.

are synthesized and exported from the cell during the initial stages of infection.

Endotoxins  can be regarded as complex lipopolysaccharides , this is can be seen as the outer cell wall of (gram negative bacteria).

They are been released when there us lyses of gram-negative cells and their synthesis and exportation are from cells at the initial stages of infection.

Therefore, they cause cause septic shock.

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Match Microscope Components to the Scientists listed below.


Microscope Components:
a. Condenser
b. Side Pillar or Arm
c. Stage Adjustment Knobs

Scientists:
1. Louis Joblot
2. Anton van Leeuwenhoek
3. Robert Hooke

Answers

Answer:

Anton van Leeuwenhoek discover stage adjustment knob.

Robert Hooke discover condenser.

Louis Joblot discover side pillar or arm.

Explanation:

Anton van Leeuwenhoek was a scientist that lived from 1632 to 1732 that observed microorganisms using microscope. He used single lensed design microscope fo his experiments. He developed the adjustment knob that magnified over 200 times with brighter and clearer images.

Robert Hooke was a scientist born in 1635 and developed a microscope. He used biconvex lens, additional lens, eyepiece lens and tube lens.

Louis Joblot was a scientist who lived from 1645 to 1723. He was often neglected in history and his inventions were not recognised and acknowledged. He was the first to recognised and identified microbes. He developed a new modifications of microscope. Joblot’s new microscope permitted precise focusing by removing stray light and enabling the mounting of a diverse array of specimens. Joblot constructed a side pillar microscope that use diaphragm to correct aberrations.

Sort the terms and phrases as to whether they function in or describe primary growth or secondary growth.

Terms:

1.apical meristem
2. cork cambium
3. elongation
4. provides structural support
5. increases exposure to light
6. increases vascular flow
7. growth in thickness
8. occurs in plants that live many years
9. occurs in plants that live only one year
10. lateral meristem
11. vascular cambium

Groth:

a. primary growth
b. secondary growth
c. both
d. neither

Answers

Answer:

Primary Growth:

1. apical meristem

3. elongation

5. Increases exposure to light

9. Occurs in plants that live only one year

Secondary growth:

2. cork cambium

4. provides structural support

6. increases vascular flow

7. growth in thickness

10. lateral meristem

11. vascular cambium

Both:

8. occurs in plants that live many years

Explanation:

Plant primary growth starts with successive cell division cycles in undifferentiated cells that form the shoot apical meristem, thereby lengthening roots and stems. The meristems represent a type of tissue involved both in primary and secondary growth. Herbaceous plants have only primary growth and grow in length.  

Secondary growth is caused by the growth of the vascular cambium (cambia) and lateral meristems. It is characterized by an increase in thickness of stems and roots. Vascular plants (i.e., plants with a xylem and phloem system) have secondary growth.

In woody plants, the primary and the secondary growth patterns occur across years, and thus they can increase their length (primary tissue growth) and diameter (secondary tissue growth).

1 point
The topsoil covering an area of land was washed away by
rain. The land was most likely-
O steep, without vegetation
O steep, with vegetation
O flat, without vegetation
O flat, with vegetation
1 point

Answers

Answer:

steep, without vegetation

tell me what you think of adolescence as toddlerhood of adulthood ​

Answers

Answer:

Adolescence is the period of transition between childhood and adulthood. It includes some big changes—to the body, and to the way a young person relates to the world.

The many physical, sexual, cognitive, social, and emotional changes that happen during this time can bring anticipation and anxiety for both children and their families. Understanding what to expect at different stages can promote healthy development throughout adolescence and into early adulthood.


What is the approximate carrying capacity of the
population?
In which year, did the population reach the
carrying capacity?
About how many years did it stay at carrying
capacity?

Answers

Answer:

250 individuals

year 3

4 years

Explanation:

Answer:250

year 3

4 years

Explanation:

given a container of a solution of 5% hcl all samples tested from this same bottle will have the same mass ratio of hydrogen to chlorine. this application of the law of

Answers

Answer:

The law of conservation of mass.

Explanation:

This law talks about that the mass is not lost during the reactions, but is maintained.

Answer:

ITS NOT CONVERSATION OF MASS. ITS CONSTANT COMPOSITION

Explanation:

which of the following occurs after tsunami and forest fire?

a) primary succession
b) quaternary succession
c) secondary succession
d) tertiary succession

Answers

Answer:

secondary succesion

Explanation:

C)Secondary succession occurs after a tsunami and forest fire.

Does secondary succession occur after a forest fire?

Secondary succession begins when a disturbance (together with wind storms, insect outbreaks, logging, avalanches, bulldozers, or fireplace) leaves the soil intact. Seeds, spores, and roots generally stay as nicely. sites that start with secondary succession reach the following degree greater quickly than during primary succession.

Secondary succession happens while a climax network or intermediate community is impacted with the aid of a disturbance. This restarts the cycle of succession, however not returned to the start soil, and nutrients are still gifts.

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Which is a function of nucleic acids? create movement provide structure store genetic information fight disease

Answers

Answer:

store genetic information

Storage of genetic information is the main function of nucleic acids. As the nucleic acid contains genes that codes for certain characters of the humans that are being inherited from parents to offspring. The correct option is C.

What are nucleic acids?

Nucleic acids are chemical compounds that arise innately and are the primary information-carrying substances in cells.

They are extremely significant in guiding protein synthesis. Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are indeed the two primary kinds of nucleic acids.

There are many organisms that contains RNA as a genetic material, and some have DNA as genetic material.

Nucleic acids play an important role in the stockpiling and expression of genomic information. DNA, or deoxyribonucleic acid, encodes the information that cells require to produce proteins.

Thus, the correct option is C.

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A woman with type A blood (whose father was type O) has children with a man that has type O blood. Both individuals are heterozygous for the MN antigen. Recall that MN blood group antigens are independent of the ABO locus, and that the alleles are codominant.
How would the results from Part A change if both parents are also heterozygous for the FUT1 gene controlling the synthesis of the H substance (Hh)?
Drag the correct value to the blank following each offspring type.
Type A with M antigen:_________.
Type A with M and N antigens:_________.
Type A with N antigen:__________.
Type O with M antigen:__________.
Type O with M and N antigens:__________.
Type O with antigen:_________.
Options: 0, 1/32, 3/32, 5/32, 6/32, 10/32, 1

Answers

Answer:

Explanation:

A woman with type A blood (whose father was type O) meaning her genotype is AO mates with

Man that has type O blood (OO genotype)

Both are heterozygous for MN blood group and both also heterozygous for the FUT1 gene controlling the synthesis of the H substance (Hh)- which determines the expression of the A and B antigen.

Cross

A O M N H h

O AO OO M MM MN H HH Hh

O AO OO N MN NN h Hh hh

Type A- 1/2 O-1/2 type M- 1/4 MN-1/2 N- 1/4, type H- 3/4 h-1/4

Type A with M antigen:

1/2*1/4*3/4 = 3/32

Type A with M and N antigens:

1/2*1/2*3/4 = 3/16

Type A with N antigen:

1/2*1/4*3/4 = 3/32

Type O with M antigen:

1/2*1/4*3/4= 3/32

Type O with M and N antigens:

1/2*1/2*3/4 = 3/16

Type O with N antigen:

1/2*1/4*3/4 = 3/32.

The 3/4 value comes from the expression of Hh-3/4 (this determines if the A and B Angie will be expressed).

What is a major benefit of schooling? reproduction hydrodynamic efficiency division of labor aerodynamic efficiency

Answers

Answer:Hydrodynamic efficiency!

Explanation:

The correct answer is hydrodynamic efficiency.

What are the major benefits of school?

Educated people have a high income, many opportunities for life, and tend to be healthy. There are also benefits to society. In a well-educated society, crime rates are low, overall health is good, and there is public involvement. Lack of access to education is considered the root of poverty.

"The main purpose of American schools is to provide all learners with the greatest possible development and to be able to live morally, creatively, and productively in a democratic society."

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1. 4. Do some research and describe two or three scenarios in which it would be preferable to use a stab tube vs. a growth plate. (Hint: What do bacteria use to help them move? Can motility be used to help identify many medically important pathogenic bacteria such as the Enterobacteriaceae?)

Answers

Answer and Explanation:

If we want to isolate a species or identify it, we should grow it in a plate with the appropriate media. In this way, it will be much easier to separate a single cell and analyze it by its morphology or study the colony growth. Stab tubes can be used when we want to study, for example, how a bacteria might grow under low-oxygen conditions. Cells might be inoculated in a liquid media or semi-solid media. We might analyze their growth in the deeper parts of the tubes, where oxygen is very low.   Grow bacterias cultures in agar plates will also allow us to separate the species of interest form other species present in the culture. We might also compare and study differences between species growth. When growing bacterias on growth plates, they do not last long, as they have more chances of dehydrating. Instead, if we want the culture to survive for a longer-term, we should use stab tubes. Stab tubes are better to study the motility of different species, which might help us to identify certain bacterias of interest.

when a true breeding plant with purple kernels was crossed with a true breeding plant with yellow kernels the f1 cob had kernels that were all purple.
a what is the dominant triat in this cross?How can u tell?
b write down the phenotypes of the parents of f1 individuals using appropriate symbols​

Answers

Answer:

fffffefwefwefwefwefwefwef

Explanation:

fwefwefwefiowejfiwejflkfjsdkljfkldsjfsdkljfksdljf

Each trait of a plant is determined by _____.
A) an allele
B) one gene
C) a pair of genes
D) one dominant gene

Answers

Answer:

c. a pair of genes

Explanation:

Answer:

C) a pair of genes

Explanation:

C should be correct because each trait is controlled my multiple genes. It is very rare to see one thing being controlled strictly by one gene, rather multiple things work together to produce the traits. Living things are very amazing if you dive deep into their DNA level,

there are more hydrogen atoms in living organisms than any other atoms are asking is more abundant in the terms Mass why is this statement true​

Answers

Answer:

The statement is true because most organisms are made of water, and water consists of mostly hydrogen (atoms) since its chemical formula is H2O.

What causes scientists to disagree? A. Many scientists do not use the scientific process. B. Some scientists are better than others. C. They have unique perspectives and skills. D. Scientists disagree to get attention.

Answers

Answer:

I would say C) They have unique perspectives and skills.

Just because everyone doesn't think the same things, everyone have a different opinions. (:

20. Which action removes carbon dioxide from the body?
exhaling
sneezing
swallowing
inhaling

Answers

Answer:

exhaling

Explanation:

when inhaling oxygen is taken in and exhaling carbon dioxide is taken out

Answer:

exhaling

Explanation:

This is because exhaling is the process of removing carbon dioxide from the body .

45-year-old was admitted with sarcoidosis of both lungs. An extracorporeal perfusion of donor lungs, from an unrelated donor, and successful bilateral lung transplantation were completed without any complications. What is the second code you should assign

Answers

Answer:

The second code in this case is the procedure for perfusion

Explanation:

Extracorporeal perfusion is the process of diverting the patient's blood via an artificial circulatory device to enable the gas exchange (oxygen absorbed, carbon dioxide removed) to take place. Only medical experts should handle this procedure so as to avoid any and all undesired scenarios or negligence towards patient care.  

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10 The mass of a cube of side 2 cm is given as 100 g. The side is accurateto the nearest millimetre and the mass accurate to the nearest gram.Calculate the maximum and minimum possible values for the density ofthe material (density = mass = volume). Which of the following numbers is a whole number? 1.) 0.3 2.) -2/3 3.) -8 4.) 3 The text below is from a US government act in 1882"Be it enacted by the Senate and House of Representatives of the United States of America in Congress assembled, That from and after the expiration of ninety days next after thepassage of this act, and until the expiration of ten years next after the passage of this act, the coming of Chinese laborers to the United States be, and the same is hereby, suspended;and during such suspension it shall not be lawful for any Chinese laborer to come, or having so come after the expiration of said ninety days to remain within the United States.This government act was passed in response to which of the following? A) nativist laborers in the North who lobbled to CongressB) complaints from immigrants who came from European countriesC) violent behavior from Chinese Immigrants living in the NorthD) nativist protests in the West against Chinese immigrant laborers Rewrite in radical form PLEASE HELP Two Brothers Moving prepared the following sales budget:Month Cash Sales Credit Sales March $20,000 $10,000April $36,000 $16,000May $42,000 $40,000June $54,000 $48,000Credit collections are 25% in the month of sale, 60% in the month following the sale, and 10% two months following the sale. The remaining 5% is expected to be uncollectible. What are the total cash collections in May at Two Brothers Moving? A) $62, 600 B) $20, 600 C) $65,000 D) $76, 100 Find the surface area of the cone in terms of pi. How is kerning used in Word? What does the phrase "dependent on" mean in this sentence?There is evidence that youth may be sensitive to nicotine and that teens can feel dependent on nicotine sooner than adults.ill fromcurious aboutaddicted tointerested in Chandni Sharma, a student in our class, is running for a political office and must give a speech that will lay out the details of her platform. The speech will be widely covered by newspapers and television. What kind of delivery is the candidate most likely to use? Can I please get help with this? Microsoft excel was used on a set of data involving the number of defective items found in a random sample of 46 cases of light bulbs produced during a morning shift at a plant. A manager wants to know if the mean number of defective bulbs per case is greater than 20 during the morning shift. She will make her decision using a test with a level of significance of 0.10. The following information was extracted from the microsoft excel output for the sample of 46 cases: n=46, Arithmetic mean=28.00, Std Dev =25.92, standard error=3.82, Null hypothesis: H0 : u Engine oil (unused) flows at 1.81 x 10^-3 kg/s inside a 1-cm diameter tube that is heated electrically at a rate of 76 W/m. At a particular location where flow and heat transfer are fully developed, the wall temperature is 370K. Determine: a. The oil mean temperature. b. The centerline temperature. c. The axial gradient of the mean temperature. d. The heat transfer coefficient. What is the percent composition of muscovite mica if its chemical formula is (KF)2 (Al2O3 )3 (SiO2 )6 (H2O) Which is equivalent to 3/8*1/4x Find the 24th term of an arithmetic sequence for which a_1 = 2 and d = 6. Please answer this correctly While Angela is making modifications to Katies Word document, she would like to inform Katie of the reasoning for the change. Which feature should Angela use? Track Changes email Comments Save File At the close of the American revolution, the United States was the most democratic country in the world. True or false? simplify expression 4(x^2)^4 x (-x)^3 what is the relationship between the exponents of (3^2) ^4 and the exponents of 3 ^8