Task 1

In this task you will analyse an RFLP in order to determine which family members carry an inherited genetic trait. This variant is thought to cause cheek dimples! The DNA sequence analysed is as follows

Normal
5΄-ATGTGCTGGATGACAGATGTCGACCACCCATGTAGTTGTGTAGACCCCCCAGT-3΄

Variant
5΄-ATGTGCTGGATGACAGATATCGACCACCCATGTAGTTGTGATACCCCCCCAGT-3΄

The restriction enzyme that was used in the analysis is called EcoRV and it recognises and cuts the following sequence 5΄GATATC 3΄.

The following family members were tested:
1. Maternal grandmother
2. Paternal grandmother
3. Paternal grandfather
4. Mother
5. Father
6. Child A
7. Child B
8. Child C

Below is the agarose gel electrophoresis results following the RFLP analysis.

1 2 3 4 5 6 7 8









(Note: the banding patterns for family members 1,2 and 4 are the same, and banding pattern for family members 3, 5, 6 and 7 are the same.)

Questions

a. Looking at the two versions of DNA, the normal and the variant, explain which one you expect to be restricted by the enzyme. (2 marks)
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

b. Give the affected family members and explain you answer. (5 marks)
__________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

c. Hypothesise why the banding pattern for child C shows three bands. (2 marks)
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

d. Which feature of DNA allows for its movement through an agarose gel when an electric current is applied? (1 mark)
___________________________________________________________________________

e. What is the reason for this feature in DNA? (1 mark)
___________________________________________________________________________



f. If the following sequence was restricted by the above enzyme, how many bands would appear on the gel? Explain your answer. (3 marks)

5΄ TGTCACTGATCGTCAGATGATATCACTGGTCCCAATCTGATC 3΄

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

g. In the diagram showing the DNA sequence above, what is the strand that is not shown called and what is the direction of this strand? (2 marks)
___________________________________________________________________________

h. Is it important to always show this missing strand in diagrams? Explain your answer. (2 marks)
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

i. Give a reason for why RFLP analysis is not as commonly used anymore for such analysis and give one technique that has replaced it. (2 marks)
__________________________________________________

Answers

Answer 1
Om said that he had a lot more than a little more money than his wife and I was in the middle of a meeting with the guy that was going through the house and I had to go back and forth with him

Related Questions

Carbon is an organic element found in all life forms. Which of the following occurs through the process of
breaking down carbon-based molecules?
A. Water molecules are transferred through the body.
B. Elimination of waste products occurs.
C. Energy needed for life functions is released.
D. Absorbing of oxygen through pores occurs.

Answers

Answer:

C is the Answer.

Explanation:

Carbon is an organic element which when broken down will release energy within an organism.  

Process of elimination

A: Wrong because Water has nothing to do with carbon being broken down.

B: Wrong because when carbon is broken down no waste products are being eliminated.

C: Right because Energy is released when Carbon is broken down

D: Wrong because Carbon being broken down has nothing to do with oxygen being absorbed.

A garden contains 40 flowers, 30 of which are red. What is the frequency of red flowers? (1 point)
O 0.5
O 1.0
O 0.25
0 0.75

Answers

the answer is the last option (0.75) which means 75% of flowers in the garden is red. you divide 30 by 40 and you get the answer

describe the climate in coastal area near a warm ocean current

Answers

Answer:

In coastal regions, cooler ocean currents tend to cause cooler land temperatures, and warmer ocean currents tend to cause warmer land temperatures in coastal regions.

Explanation:

(brainliest plz)*

Which of these are you likely to find in a bad land

Heavy rainfall, hardwood trees, peat, or erosion

Answers

Answer:

Explanation: erosion

Fossil fuels are the carbon remains of plants and animals that died long ago.
True
False

Answers

Answer:

The answer is True

Explanation:

Answer:

True

Explanation:

They are remains of died plants and animals

I’ll give points + brainalist for the correct answer (:

Answers

[NAA]

Excuse me, i can't quite see the question and the answers.

May you please ask again?

[xArtsy]

2
Which statements about humus are true? Choose more than one
answer
It forms from dead plants
It collects in bedrock
It fertilizes soil
It contains carbon
It is only found in topsoil
It erodes the rocks in soil

Answers

It forms from dead plants

It fertilizes soil

It contains carbon

It is only found in topsoil (not sure abt this tho)

Humus forms from dead plants, fertilizes soil, contains carbon, and is only found in topsoil. The correct options are A, C, D, and E.

What is humus?

When plant and animal debris decomposes in the soil, humus, a black, organic substance, is created.

Plants scatter leaves, twigs, and other debris to the ground, which accumulates. Leaf litter is the name of this substance. Animal carcasses contribute to the litter after they pass away.

Humus is a form of soil produced by decomposing organisms and plants.

The name was developed by a French soil expert named Muller and is based on the Latin word "soil". Humus is challenging to define and is still not completely understood.

Only found in topsoil, humus is a material that develops from dead plants, fertilizes the soil, and contains carbon.

Thus, the correct options are A, C, D, and E.

For more details regarding humus, visit:

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Decomposers are important because they:
recycle dead material
take nutrients from the soil
help sick plants and animals die
all of the above
ANSWER ASAP FOR BRAINLY

Answers

Answer:

They recycle dead material

Explanation:

They use dead plants and animals and the nutrients from that gets put back into soil it helps living plants

Good luck <3

Which of the following occurs during
telophase and cytokinesis?
A. the division of the cell's nucleus
B. the cell's DNA is copied
C. the division of the cell's cytoplasm
D. cell division into two identical daughter cells

Answers

cytokinesis is when the cell divides

telophase is when it coppies itself befor it splits

i hope that maybe helped a small bit atleast

what is the essence of recognizing the different species of organism in our country 1 paragraph pls

Answers

Answer:

To maintain equilibrium.

Explanation:

The essence of recognizing the different species of organism is very important in our country because we can avoid certain activities that leads to the extinction of that species. Recognizing the nature of a specie is necessary in order to provide good and suitable environmental conditions to the organisms and avoid those activities due to which its growth is negatively affected and leads to removal of that specie from ecosystem so that's we can say that the essence of recognizing the different species of organism is very important to maintain equilibrium in the ecosystem.

6. What is the most important factor used in determining an igneous rock's texture?

Answers

Answer:

The texture of a rock refers to the size, shape and arrangement of the mineral grains in the rock that give the rock its overall appearance. The most important, but not only, a factor that controls the grain size of a rock is its cooling history.

Answer:

its multiple

Explanation:

smoothness with glass like features

Discuss the orogenies that produced the Appalachian Mountain Range. Include in your answer the timing, process, and resultant sedimentation patterns. Include additional Paleozoic orogenic events to strengthen your answer if need be.

Answers

Answer:

Explanation:

Appalachian orogenic belt, an old mountain range that extends for more than 3,000 km (1,860 miles) along the eastern margin of North America from Alabama in the southern United States to Newfoundland, Canada, in the north. The geosynclinal theory of mountain building was first worked out in the Appalachians by James Dana and James Hall in the late 19th century; today a plate tectonic theory is invoked. The earliest Appalachian sediments were deposited near the start of the Cambrian Period (542 million years ago) on the shores of the opening Iapetus Ocean. Subduction of the Iapetus led to its destruction and the collision of different continental blocks and island arcs. Those collisions gave rise to three Appalachian orogenies: the Taconic in the Middle Ordovician (about 472 million years ago); the Acadian in the Middle to Late Devonian (at 390 million to 370 million years); and the Alleghenian in the Late Carboniferous to Permian (300 million to 250 million years ago). The age of these orogenies decreases eastward across the orogenic belt, demonstrating that it was formed by the progressive eastward addition of arcs and continental fragments to the continental margin of North America. The Appalachian belt continues to the east in the form of the Caledonian and Hercynian orogenic belts in western Europe. The Alleghenian orogeny led to the formation of the Pangaea supercontinent during the Permian Period (299 million to 251 million years ago). Geophysical seismic studies show that the southern Appalachian Mountains comprising the Ridge and Valley region, the Blue Ridge Mountains, and the Piedmont region belong to a crustal slab some 6–15 km (3.7 to 9.3 miles) thick that has been thrust 260 km (162 miles) westwards over the former continental margin.


What role do bacteria play during the nitrogen cycle?
A( Bacteria turn nitrogen into phosphates
Bacteria store nitrogen in wastes
C( Bacteria turn nitrogen gas into a form that living things can use
D( Bacteria convert nitrogen into water

Answers

Answer:

Bacteria turn nitrogen gas into a form that living things can use

Which space object releases the most electromagnetic radiation?
A. Comets
B. Jupiter
C. The Earth
D. Stars​

Answers

Answer:

The Stars release most.

True or false please be experienced and know what you are doing. If you are not good at life science please skip this question.

Answers

well, i think the first one is false because plants dont produce sexually

the second one is false

third- please fact check me ut i think its true

forth is true

true

true

true

true

true

please fact check me i grow plants but im not a scientist lol

1. How does bedrock relate to stream drainage patterns?
OA.
Where there are differences in bedrock, streams will tend to develop where bedrock is least resistant to
weathering and erosion.
Where there are differences in bedrock, streams will tend to develop where bedrock is most resistant to
OB.
weathering and erosion.
O C. Streams develop in sedimentary rock but not in igneous or metamorphic rock.
OD. Streams develop only in areas where bedrock is uniform

Answers

Answer:

a

Explanation:

the bedrock is resistant therefore it will streams will start to form

the answer is A i hope you got it right

If a natural hazard only harms plants (like consumers), how will that impact producers????

Answers

Well plants are producers so if your taking about a natural hazard harming producers is would harm consumers as it would deprive them of food.

If you taking about the harming of consumers (animals), then producers would be impacted as they would over-populate causing competition within the species of producers. The plants would experience exponential growth but eventually reach its carrying capacity due to competition and resource depletion.

In a straight stream, where does the water travel the fastest?

Answers

Answer:

In the center of the stream well above the bottom of the bed or channel but below the surface.

Una mujer cuyo padre era Daltónico se casa con un hombre cuyos padres no tienen antecedentes para dicha anomalía visual.Este matrimonio tiene 2 hijos y 2 hijas.¿Qué porcentaje de los descendientes podría presentar daltonismo?

Answers

Answer:

4 porcentaje

Explanation:

4 o 8 porcentajes

FDSGSDFSFFSDFSDFSDFSDFSFSDFSDFSDFSDFDSFSFSFSDF

Answers

Answer:

hey Bestie

Explanation:

George Washington said hello

A scientist helps a colleague do a better experiment by:
O A. laughing when a common and avoidable mistake is repeated.
B. offering advice about how best to perform a standard procedure.
C. explaining why a hypothesis does not need to be tested in an
experiment.
D. refusing to allow anyone else to use an expensive instrument.

Answers

Answer:

B. Offering advice about how best to perform a standard procedure.

Explanation:

Helping others can be in different ways, be it helping out or by giving encouraging words or advice. But in all aspects of help, there is always a positive outcome if help is given in the right way and at the right time. And it is also how one person lifts another.

A scientist can help a colleague out by offering advice and telling the person how to best perform a standard procedure. This will help the colleague do better in the experiment and also help ensure that the right things are done and the efforts are successful. The other options are all negative in their 'approach' which will only hinder the colleague in his/her works.

Thus, the correct answer is option B.

Answer:Offering advice about how best to perform a standard procedure.

Most of the Earth's oxygen occurs in the form of minerals in the crust and mantle. The oxygen that is available in the atmosphere is
used primarily by plants, animals, and bacteria to perform cellular respiration.
Why is running out of oxygen still not a real concern?

A. Oxygen is recycled through the interrelated processes of photosynthesis and cellular respiration.
OB. Oxygen can easily be mined from rocks and sand and then be released into the atmosphere.
the
OC. The Sun creates more than enough new oxygen atoms in the atmosphere by splitting phosphate molecules.
OD. Lightning creates large quantities of ozone, which quickly breaks down into oxygen molecules.
DONT TELL ME LINK

Answers

Answer:

I think A

I hope this helps!

Explanation:

A student conducts an experiment on the effects of temperature on seed growth. In a notebook, she draws a picture of the seeds on day 10 and writes that the seeds left in the warmest location have had the largest growth.

What part of scientific investigation best defines these actions?


observations

logical reasoning

hypothesis

evaluating

Answers

Answer:

yellow Read the paragraph.

Jess and her family are moving to a new city. Jess has never lived anywhere else in her life and wonders what the change will be like. She will miss her friends and the familiar sights. However, Jess has always liked trying new things. Her parents said there is a park right next to their new home and a yard big enough for a dog. Jess is interested in her family’s new adventure.

Which phrase best replaces interested in to show how Jess is feeling in this situation?

excited about

uncomfortable with

nervous for

surprised by

Explanation:

yellow wc

The best part of scientific investigation that best defines the action where a student conducts an experiment on the effects of temperature on seed growth and draws a picture of the seeds on day 10 and writes that the seeds left in the warmest location have had the largest growth is observation. The correct option is A.

What is scientific investigation?

A scientific investigation is an effort to find explanations for observed phenomena. In general, the scientific investigation follows a step-by-step procedure that identifies a problem to be resolved, collects relevant information, formulates a hypothesis, tests the hypothesis by experimentation, analyzes the information collected, and makes conclusions.

The primary goal of scientific research is to extend the knowledge base of a field of study. The best part of scientific investigation that best defines the action where a student conducts an experiment on the effects of temperature on seed growth and draws a picture of the seeds on day 10 and writes that the seeds left in the warmest location have had the largest growth is observation.

Observation is a collection of data by examining something or someone. It involves looking for particular information or signs of activity, recording the data obtained in a systematic manner, and utilizing the data to draw conclusions or create predictions.

Therefore, the correct option is A.

Learn more about Scientific investigation here:

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If a mutation introduces a new skin color in a lizard population, which factor might determine whether the frequency
(presence) of the new allele will increase?
A)The size of the population
B)The fitness benefit of the mutation
C)How many other lizards have the mutation
D)Whether or not the mutation occurred naturally

Answers

C because new skin color lizards will cause more new mutations

Part A Think about the parts of an egg, and write down why you think it can act as a model of an animal cell. Also write down your predictions of what will happen when you leave an egg in vinegar for three days.​

Answers

Answer:

An egg mostly has 3 parts being the Shell, the Egg white and the Yolk.

This is almost like the animal cell which has three main parts being the Cell wall, the Cytoplasm and the Nucleus.

Similarities that enable the egg to be a model of animal cell:

Both cell membrane and egg shell are selectively permeable which means they only allow certain things in and osmosis can occur. Cytoplasm in animal cell surrounds the nucleus like the egg white surrounds the yolk. The nucleus is more or less in the center of the animal cell much like a yolk is more or less in the center of an egg.

When an egg is left in vinegar for three days, osmosis will occur and the egg shell will probably dissolve. This means that because vinegar has a higher concentration of water than the egg, water molecules will move from the vinegar to the egg across the egg shell leading to the egg increasing in size.

As the egg shell absorbs more vinegar, it will end up dissolving.

How can bacteria share molecules that impact their ability to survive?

Answers

Answer:

a host cell its what they feed off of

Explanation:

hope this helps a bit :)

Why do you guys think there are so many insect species still undiscovered?

Answers

Answer:

mutations and undiscovered locations

Answer:

Because there were multiple gene mutations and adaptations to new habitats.

______ occurs because the nervous systems send messages to the muscles in the muscular system.
(I need someone to fill in this blank with the best possible answer, I’ll reward points + brainalist)

Answers

Answer:

action potentials

Explanation:

other possible answers:

electrical impulses

muscular contractions

action potentials is likely your best bet though

Explanation:

Neurons carry messages from the brain via the spinal cord. These messages are carried to the muscles which tell the muscle fibre to contract, which makes the muscles move.

1. How do Milankovitch cycles affect global temperature

Answers

As obliquity decreases, it gradually helps make our seasons milder, resulting in increasingly warmer winters, and cooler summers that gradually, over time, allow snow and ice at high latitudes to build up into large ice sheets.

Which of the following is NOT characteristic of those who engage in coercive or aggressive sexual activity?
a. They have high levels of traditionally masculine traits
b. They have unusually high levels of sexual interest
c. They tend to rationalize their aggressive behavior
d. They were victims of childhood violence or sexual abuse

Answers

Answer:

c. They tend to rationalize their aggressive behavior

Explanation:

Individuals who involve aggressive sexual activity having high levels of traditionally masculine traits. These type of people experience sexual abuse, witnessing family violence in their childhood that leads to aggressive sexual activity. These people have unusually high levels of sexual interest. Use of illegal drugs, use daily alcohol use, and excessive time spent "hanging are the factors that are responsible for aggressive sexual activity.

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