Your student is trying to create a "Gene X" knockout mouse by introducing a recombinant construct into embryonic stem (ES) cells, selecting transformants, and introducing the transformed cells into mouse blastocysts. He designed his construct as follows: NeoR region of homology to Gene X---HSV tk--- region of homology to Gene X where Neo is the neomycin-resistance gene and HSV tk is the Herpes Simplex virus thymidine kinase gene. He transforms the ES cells and exposes them to G418 and ganciclovir. Explain to your student why he failed to recover transformed ES cells. Choose the two correct statements. a. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be sensitive to ganciclovir and neomycin, and would die.
b. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be resistant to ganciclovir, but sensitive to neomycin, and would die.
c. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to ganciclovir, but resistant to neomycin, and would die.
d. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to neomycin and ganciclovir, and would die.
e. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to neomycin, but resistant to ganciclovir, and would die.
f. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be resistant to neomycin, but sensitive to ganciclovir, and would die.

Answers

Answer 1
Your student is trying to create a Genex

Related Questions


HELP
Youngest to oldest

Answers

Answer:

E  B  I F H A D G C

Explanation:

Which of these biological communities has permafrost? A. Tundra B. Grassland C. Desert D Forest​

Answers

Answer:

the tundra biome :)

Explanation:

grass lands aren't very cold

forests aren't cold

the desert is hot

and tundra is super cold

A. Da tUnDrA c;
ur welcome

Guys helpppppp please and I hope y’all have a great day

Answers

Answer:

Ok so

Explanation:

1. DNA

2.A , G , C and T

3.The 3-dimensional double helix structure of DNA, correctly elucidated by James Watson and Francis Crick. Complementary bases are held together as a pair by hydrogen bonds.

4.Chargaff's realization that A = T and C = G, combined with some crucially important X-ray crystallography work by English researchers Rosalind Franklin and Maurice Wilkins, contributed to Watson and Crick's derivation of the three-dimensional, double-helical model for the structure of DNA.

5.Created by Rosalind Franklin using a technique called X-ray crystallography, it revealed the helical shape of the DNA molecule. Watson and Crick realized that DNA was made up of two chains of nucleotide pairs that encode the genetic information for all living things.

6. 1869

7.The double helix of DNA is, like its name implies, in the shape of a helix which is essentially a three dimensional spiral. The double comes from the fact that the helix is made of two long strands of DNA that are intertwined—sort of like a twisted ladder.

8. In DNA, the code letters are A, T, G, and C, which stand for the chemicals adenine, thymine, guanine, and cytosine, respectively. In base pairing, adenine always pairs with thymine, and guanine always pairs with cytosine.

9. A gene is the basic physical and functional unit of heredity. Genes are made up of DNA. Some genes act as instructions to make molecules called proteins. ... Every person has two copies of each gene, one inherited from each parent.

10. 20

11. A group of disorders that cause red blood cells to become misshapen and break down.

With sickle cell disease, an inherited group of disorders, red blood cells contort into a sickle shape. The cells die early, leaving a shortage of healthy red blood cells (sickle cell anaemia) and can block blood flow causing pain (sickle cell crisis).

12. Sickle cell anemia is caused by a mutation in the gene that tells your body to make the iron-rich compound that makes blood red and enables red blood cells to carry oxygen from your lungs throughout your body (hemoglobin).

13. The Human Genome Project was an international scientific research project with the goal of determining the base pairs that make up human DNA, and of identifying and mapping all of the genes of the human genome from both a physical and a functional standpoint.

I'm so absolutely sorry if this is long. Good lucj with ur work ;D

If more snakes moved into this environment, then
A. the number of rabbits would go up.
B.the number of grasshoppers would go up.
C. the number of toads would go up.
D. The number of hawks would go up.



NO LINKS AND DO NOT ASK A QUESTION UNDER MY QUESTION ❗❗❗​

Answers

Answer:

B. The number of grasshopers would go up.

Explanation:

A. is incorrect because it would decrease

C is incorrect  because it would also decrease

D is incorrect because snakes dont eat Hawks, but Hawks eat them

B is correct because since snakes would eat the toads, there would be no toads eating grasshoppers

Hope this helps!!!

Male damselflies fight other male damselflies of their own species to win a mate. This shows that only the strongest can
O have enough to eat
O impact energy
O generate a defense
o pass on their traits

Answers

Answer:

Hello There!!

Explanation:

I believe the answer is=>O have enough to eat.

hope this helps,have a great day!!

~Pinky~

Why does sound not literally blow us away?

Answers

Answer:

i.d.k what?

Explanation:

What example best compares to the relationship between genes and chromosomes? PLZ Somebody help
A. A gas tank in a car because it stores energy.
B. Skin on a human because it protects the body.
C. Leaves on a tree because they provide energy.
D. A recipe in a cookbook because it gives directions.

Answers

Answer:

b

Explanation:

Answer:

D

Explanation:

I think its d

Help someone helppppp please omgggggg ahhhhh!!!!

Answers

I think weather Bc I’ve read that that happens from wind

Fiona wants to use the scientific method to answer the question “Do oxygen levels in the air affect rates of photosynthesis?” She reads about photosynthesis in her biology book and is ready to carry out the next step. What should she do next?
perform an experiment
design a procedure
make observations
form a hypothesis

Answers

The first thing Fiona, or anyone, should do is form her hypothesis.

The hypothesis is your own proposed explanation for whatever phenomenon you're trying to study. Think of it as a predetermined idea of what could happen based on what you already know.

Answer:

The correct answer is D!:)

Explanation:

Hope this helps!:)

Calculate the number and percentage of eukaryotic species that are still to be formally described?

Answers

the number and percentage of eukaryotic species that are still to be formally described is to be beauty

Eukaryotic organisms are species that have defined nucleus and membrane limited organelles. Total 11% or 250,000 are described while 97% or 8490000 are yet to be described.

What is the population of the eukaryotes?

Eukaryotes are the largest and the most dominating species that includes kingdom plants, animals, fungi and protists. A total of  8.74 million eukaryote species on Earth are present from which only 250,000 are described.

Eukaryotes are the species that are well-defined organisms that contain the genetic material enclosed within the nuclear membrane and the organelles are also enclosed.

With new discoveries and findings, many new species are described that are grouped into one of the kingdoms based on their properties and features.

Therefore, 8.74 million eukaryote species are present on Earth.

Learn more about eukaryotes here:

https://brainly.com/question/4944603

Why do so many more people have brown eyes then blue eyes

Answers

Because of genetics duh

Which of these is an example of the conversion of gravitational potential energy into kinetic energy?

Group of answer choices

a wind-up spring making a toy car move across the floor

a person running on a level track

an apple falling from a tree to the ground

a rubber band being stretched to twice its normal length

Answers

I think it’s a rubber band being stretched to its normal length

I’ll mark u as brainliest! Tell me what definition goes where ?!

Answers

1. Two traits blend...
2.Two traits are both....
3. Determined by...

Answer:

Codominance- Two traits are both present in a third phenotype.

Incomplete Dominance- Two traits blend to form a third phenotype.

Sex-Linked Traits- Determined by gene located on sex chromosomes.

Explanation:

I hope this helps.

would occur if we were suddenly thrown back into the “Dark ages” with no use of technology

Answers

So there are a lot of people who say that the Dark Ages, lasting about 1,000 years, set humans back technologically. However, some say that since the Dark Ages really only affected Western Europe, and not the Middle East or Asia, it wouldn't make a difference.

If there had been no "Dark Ages" there would have been no Renaissance. Historians actually argue about when Renaissance started, and the answer is than it is not possible to pinpoint a date. The Crusade did more to bring the eastern world to western Europe than they did to divide.

Chomosomes are duplicated during

Answers

Answer:

Chomosomes are duplicated during interphase

Brainliest?

Chromosomes are duplicated during the S phase of interphase.

What part of the ice cores tells us what the atmosphere was like in the past?
A.The water when the ice cores melt.
B. The ice crystals.
C. The rings of the ice core.
D.The air bubbles trapped in the ice cores.

Answers

Answer:

C

Explanation:

I took the test a few days ago. :)

True or false Question #1 Aspen has just learned HPV. (the human papillomavirus),
which can cause genital warts and cancer is so common most seually-
active people get at least one type of HPV during their lives. Aspen is
cared about HPV and has discovered there is a vaccine for this virus.
Aspen can go to a healthcare provider to get the vaccine but has to go
back for a second dose later.

Answers

Answer:

True

Explanation: One version of the human papillomavirus, which leads to most cases of cervical cancer, evolved in humans as a result of sex with Neanderthal, a new study shows

Which of these does not provide information about the geologic history of Earth?

A) fossils


B) ash from volcanoes


C) debris from meteorites


D) current weather patterns

Answers

Answer:

is B

Explanation:

volcanic activity was more common in the past than it is today.

A trait is a distinct variable of
a character of an organism
that can be inherited. Which
of the following would be
considered a trait?
A. skin tone
B. eye color
C. green eyes

Answers

Im pretty sure that it is A!!!

Please help this is a 7th grade science problem multiple choice

Answers

Answer:

Hello There!!

Explanation:

The answer is d.Nucleus.

hope this helps,have a great day!!

~Pinky~

Answer:

The correct answer is 'd'.

Explanation:

DNA is located in nucleus.

Plasma estrogen levels reach their peak at the end of the luteal phase of the ovarian cycle.

a. True
b. False

Answers

Answer:

Plasma estrogen levels reach their peak at the end of the luteal phase of the ovarian cycle.

a. True

Explanation:

A woman's estrogen levels become highest towards the end of her ovulation.  At this point, the woman is naturally prepared for a possible pregnancy.  The occurrence of the highest estrogen levels coincides with the luteal phase, a stage of a woman's menstrual cycle, which occurs after ovulation (or the release of eggs from the ovaries), and before the start of the normal period. This luteal phase has been observed to be within 14 days for all women.  By the end of the luteal phase, the corpus luteum function declines.

Several human populations spread throughout the Sahara desert have multiple mutations in Gene R that confers heat tolerance. Independently, a small population of humans in the Mojave desert of California have a unique mutation in Gene Z that also confers heat tolerance. The evolution of adaptation of heat tolerance across all of these populations would be considered an example of _________

Answers

Answer:

Variation

Explanation:

Migration into a population from other populations with varying gene frequencies, evolution, or adaptation of heat tolerance is a cause of variation. The allele frequency of the resulting hybrid population would be right in the middle of its original value and the donor population's frequency. In tropical and subtropical ecosystems (Sahara deserts), high temperatures are a significant restriction to human population productivity; they induce morphological and physiological modifications at all times, resulting in significant yield losses but the ability and evolution of human species to cope in this region is as a result of variation.

why does secondary consumers cannot digest the bones of their prey

Answers

Answer:

The conclusion to be drawn from the fact that secondary consumers do not digest the bones of their prey is that energy used to create bone mass often does not travel farther up the food chain . Explanation: ... No energy is required to form bones .

Explanation:

Answer:hebivore

Explanation:

how should a detective work with forensic scientists in order to solve a crime?

Answers

1 First Steps: Observation and Data Collection. As a criminal investigator, you should begin by making observations. ...

2 Create Your Hypothesis. ...

3 Test the Hypothesis. ...

4 Interpret Results and Form Conclusion. ...

5 Crime Investigation Tips.

constellation are believe to ne of great help in the lives of some people.

True or False​

Answers

True
___

Explimination :

Because it is true thank me later

An experiment similar to the 1952 Hershey and Chase experiments was done in which radioisotope 35S was added to a bacteriophage replicating within its bacterial host. The new phage particles were carefully isolated and used to infect fresh bacterial cells and no radioisotopes were utilized. Based on this information, where would you expect to find the 35S radioisotope immediately after infection

Answers

Answer:

inside the phage ghosts outside the bacterial cells.

Explanation:

According to Hershey & Chase- Experiments, the radioisotope 35S is frequently associated with protein since sulfur is a protein component.  The radioactive sulfur is absorbed into the protein coat because it already contained sulfur (35S).  The radioactive marker 35S was integrated into the Bacteriophage protein coat and remained as phage ghosts outside the bacterial cells.

Which factors increase the heat absorbed by a land surface? Select the two correct answers. (1
becoming paler
becoming smoother
removing plants
removing snow
becoming wetter

Answers

The heat absorbed by the land and oceans is swapped with the atmosphere by the means of conduction, radiation, and latent heat. By removing plants, and removing snow increase the heat absorbed by a land surface, i.e., option C and D.

What are the means of heat transfer?

Heat transfer is a mechanism of conveying energy and entropy from one location to another.

It can be done by either of the following:

Conduction.Convection.Radiation.

Factors increase the heat absorbed by a land surface are by removing snow and by removing plants.

Thus, the correct options are C and D.

For more details regarding heat transfer, visit:

https://brainly.com/question/13433948

#SPJ2

Answer: The correct options would be C) removing plants and D) removing snow.

Hope this helps ^_^

b) What is DNA? What is a Gene?

Answers

Your DNA is what makes you uniquely you. It's that double helix that your genes are made of. ... DNA stands for deoxyribonucleic acid, sometimes called "the molecule of life," as almost all organisms have their genetic material codified as DNA.

Which would most likely be done while designing a solution?

Answers

Answer:

Steps of the technological design process include: identify a problem, research the problem, generate possible solutions, select the best solution, create a model, test the model, refine and retest the model as needed, and communicate the final solution.

Explanation:

This is a very vague question. Were there options?


Which of the following statements about planetary satellites is true?

Answers

Answer:

C

Explanation:

Other Questions
Mikhail S. Tswett (1872-1919) realized that ________ was an efficient method for separating compounds. The amount of water consumed each day by a healthy adult follows a normal distribution with a mean of 1.30 liters. A health campaign promotes the consumption of at least 2.0 liters per day. A sample of 10 adults after the campaign shows the following consumption in liters: 1.361.351.331.661.581.321.381.421.901.54 At the 0.050 significance level, can we conclude that water consumption has increased? Calculate and interpret the p-value.A.) State the null hypothesis and the alternate hypothesis.H0: _____H1: >_____B.) State the decision rule for 0.050 significance level. (round to 3 decimal places)C.) Compute the value of the test statistic. (round to 3 decimal places)D.) At the 0.050 level, can we conclude that water consumption has increased?E.) Estimate the p-value. Two advantages of saving money? 7. Rose can type 70 words per minute (wpm). At this rate, how many words can she type in 30 minutes? a. 3100 wpm b. 40 wpm c. 2100 wpm d. 280 wpm In current business publications, find examples of firms whose strategies to increase profits focus primarily on generating more revenue. Compare these cases with firms that are trying to cut costs to increase profits. In your initial post, include a summary of these firms and how the different strategies have been successful or unsuccessful.Having a nice size profit margin is the goal for most companies. In order to make this happen there has to be a plan/strategy that is carefully thought out and implemented. You want to take your time and make sure things are done correctly and in a way that it is profitable and not detrimental to your company at the same time. One good example of this act comes from McDonalds. One McDonalds franchise in New York increased the price of their Big Mac from $5.98 to $6.28 currently to increase revenue. Prior to this there was an anual increase that took place. "The Golden Arches' pricing in the U.S. Rose 2% year over year in the first quarter, which was still less than the consumer Price Index's overall".On the other end of the spectrum we have companies like Apple. Apple fired 1,600 full time employees from retail stores to increase their profit margin. "The retail segment reported operating income of $308 million during the second quarter of 2009 down from #334 million....". In cutting down on those full time positions they were able to increase their profits by paying less full time salaries to employees. "Revenue increase 8.7% to 8.16 Billion, which is more than 7.96 billion expected...". I do not feel that this is the best way to go about getting results. A merger of the two would be effective. Gradually increasing sales prices and not eliminating so many positions at once or merging positions even would be effective. You have to take into account that less employees mean more work for the workers that are left, which could leave them in a stressful work environment. In turn could mean customer service quality decreases drastically. Things have to be looked at on both sides of the spectrum. In thinking about Hobbes' concept of Leviathan, how do you seethis concept in action in today's society? Let's play football (into negative) Which of the following is not true of a mass customization process strategy? Can You Help Me With This Please? I need this answer quick godbless if u help Please help me answer this im being timed, tysm ur the best!:) Tim's credit card has a restriction that he must pay either 15 percent of the unpaid balance or $25, whichever is greaterWhich will he pay if he has an unpaid balance of $216?A) $25 B) 15% Write one line of code to declare a 'Path' reference variable named: pathobject4 AND create the object by calling the 'Paths' class method get and passing it: C:/programs/values.dat Write one line of code to declare a Scanner reference variable named: my_input AND create the object passing the constructor the variable: fileobject3 The Adventure of toto who is the speaker of this line What is blitzkrieg and how does it work? Inscribed angles math lib Need help with number 2 , 3 , and 4 thank you!!! Can someone please check if the labels and everything on this diagram is correct. please help me, it's urgent. I will mark brainliest, no links please I need help can someone tell me which choice is correct? hii! please help asap. ill give brainliest what happened in mexico when the oil spill from 2010how did it start and how did it end