You have isolated a mutant of Shigella dysenteriae bacteria that is resistant to kanamycin, and you want to find out what gene is responsible. Design a protocol using genetic selection to identify it

Answers

Answer 1

Answer:

It is possible to identify the function of a gene by site-specific targeted mutagenesis (i.e., by using a reverse genetics approach).  

Explanation:

Targeted mutagenesis is an approach widely used in molecular biology in order to determine the functions of endogenous genes. Targeted mutagenesis is a type of genetic technique known as reverse genetics since it is a technology that enables to identify the function of a gene by modifying its product (i.e., its corresponding messenger RNA), thereby synthesizing non-functional proteins.

Moreover, it is also possible to identify the function of a gene by altering its expression, for example, by using small interfering RNAs (siRNAs) that inhibit protein translation through RNA interference (RNAi) mechanisms.


Related Questions

Which activity is part of managing water resources?
studying water to see whether it is polluted
taking a long hot bath to stay warm
drinking water and using it for swimmig and boating
transporting water to areas where it is needed

Answers

First and Last ones I think. Not sure.

Answer: D

Explanation: Studying water isn’t managing it, and taking a long bath isn’t either. Drinking water isn't really saving or managing so D.

Which type of mutation occurs only in gametes?
germline mutation

somatic mutation

point mutation

frameshift mutation

Answers

Answer:

Germline mutation

Explanation:

A germline mutation is a genetic change produced in the gametic-germline cells (i.e., male and female gametocytes). The mutations in the germline are inherited in the next generation, while changes in somatic cells are not inherited. Moreover, the germline mutations may occur either before the fertilization or during embryonic development (zygote morula, blastula and gastrula). This type of mutation may be induced by both endogenous (e.g., oxidative DNA damage) and exogenous (e.g., exposure to UV light) factors.

The type of mutation that occurs in gametes only is : ( A ) Germline mutation

Germline mutation is a type of mutation that occurs in the gametocytes ( male and female ) and is passed( inherited ) on to the offspring ( next generation ). while mutations occurring in somatic cells are not passed on to the next generation .

Germline mutation is induced by either endogenous factors or exogenous factors and this is because Germline mutations can occur before fertilization or after fertilization.

Hence we can conclude that the type of mutation that occurs in gametes only is Germline mutation.

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Could a type B child whose mother is type A have a type A father

Answers

Answer:

No

Explanation:

It is necessary that each parent have B antigen for its child to be B type.

No this can not happen the child should be type A

Question 8 of 10
The term carbon footprint refers to the amount of carbon dioxide:
A. broken down due to a person's activities.
B. given off due to a person's activities.
C. stored by a person.
O D. consumed by a person.
SUBMIT
Pls help

Answers

Answer:

1 B carbon footprint refers to the amount of carbon dioxide given off due to the activities of particular individuals, organization or community

What was the population density of the original sample? What would have happened if you had inoculated an agar plate with 1 mL of the original sample?

Answers

Answer:

The sample was at a population density of 8.75x10^4 CFU/mL originally.

Explanation:

Had it been inoculated in the way as described in the question statement, the bacterial growth would have covered all of the agar plate and we would not have been able to distinguish individual colonies of bacteria. 1 mL is too much of a sample volume to use in cases like these.

who is the father of genetics ​

Answers

Answer:

The father of genetics is Gregor Mendel. Gregor Mendel was considered the father of genetics during the 19th century.

Hope this helps! :)

Gregor Mendel is the father of modern genetics and presents his research On experiments on plat hybridization

The specialized soft tissue manipulation technique used to ease the pain of conditions such as fibromyalgia, movement restrictions, and temporomandibular joint (TMJ) disorders is known as _____.

Answers

Answer:

myofascial release

Explanation:

The high rate of diversification of HIV strains inside a host is a result of (SELECT ALL THAT APPLY):

Answers

Question:

The high rate of diversification of HIV strains inside a host is a result of (SELECT ALL THAT APPLY):

1. High mutation rates in the HIV itself.

2. Strong and varying selection forces caused by hypermutation of antibody genes in the lymphocytes.

3. Errors in transcribing the viral RNA from the host DNA.

Answer:

All the above options are correct.

Explanation:

According to research, Retroviral cultures evolve rapidly to fit the dynamic nature of their environs.

It is also established that errors in replication can provide the basis for diversity.

Cheers!

Large-scale fires can lead to a decrease in atmospheric carbon dioxide,
Please select the best answer from the choices provided
ОТ
OF

Answers

Answer:

False

Explanation:

Fires produce carbon dioxide

which describes a meta phase plate

Answers

Answer:Metaphase plate refers to sister chromatids lining up in the center of the cell. This event occurs during the metaphase of the cell division and it is the indication of this phase. Metaphase plate is a moment when all of the chromosomes organize, line up literally in a row and after that begin to.

The Law of Overcompensation and Overload allows for which of the following in regards to intensity?
A. Progressively increase over a period to time
B. Progressively decrease over a period of time
C. Remain the same
D. Acute decrease over a period of time

Answers

Answer:

A. Progressively increase over a period to time

Explanation:

The law of overcompensation states that the environment compensates for any factor by twice the amount and the overload theory states that challenges should be increased while working on a certain task to improve the results.

The laws allow for the following:

Option A. Progressively increase over some time.

The laws can be explained as:

In fitness and sports, the laws of overload and overcompensation are widely used.

The overload principle says that the intensity and exercise duration should be increased gradually to see an improvement in endurance, strength and muscles.

According to the overcompensation theory, the body recovers from any injury or harm the body does not quit there and will be stronger compared to before.

Thus, overcompensation and overload increase over time.

To learn more about overload and overcompensation theory follow the link:

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the period during which blood is being pumped past the semilunar valves into the elastic arteries

Answers

Answer: ejection

Explanation:

The beginning of life for human beings is different from the biological beginnings of other mammals in that it involves the creation of a body and a _______.

Answers

Answer:

The beginning of life of human beings is different from the biological beginning of other mammals, since it involves the creation of a body and a brain with the power of reason.

Explanation:

The human being, unlike other animals, his brain has the ability to reason, that is, to be able to manage with an intelligence center.

There are some animals that have developed feelings and that is what makes them be able to look more like humans, but in simple words the rest of the mammals are guided by their natural instinct while man also uses the rational weapon of life. .

A chemical reaction is an interaction that always causes:
O A. matter to be changed into energy.
B. macromolecules to be formed from glucose.
O C. molecules to break apart into atoms.
O D. atoms to be rearranged into new substances.

Answers

Answer:

D. atoms to be rearranged into new substances.

Explanation:

Chemical reactions involve interaction between chemicals such that all reactants are changed into new materials. The properties of the new materials are different from those of the reactants.

Chemical reactions involve breaking chemical bonds between reactant molecules (particles) and forming new bonds between atoms in product particles (molecules). The number of atoms before and after the chemical change is the same but the number of molecules will change.

In cell biology, what does the word queue describe?

Answers

Answer:  

In cell biology, the word Queue describes the long arm of a chromosome.

Explanation:

The long arm of a chromosome is termed the q arm. All human chromosomes have two arms; the p arm which is the short arm and the q arm which is the long arm. The two are separated from each other only by primary constriction at the center called the centromere, the point at which the chromosomes is attached to the spindle fibers during cell division. 

Slowing Down Global Warming As Hite and Seitz (2016) discuss in Chapter 5: Climate Change, reputable scientific studies continue to show that the Earth is warming at an unsustainable rate because of a rise in the release of CO2 and methane gases. What is the relationship between population increases and climate change

Answers

Answer:

The increasing level of all the facilities like transportation, production, human waste, and developing new spaces for human beings among others are some of the most essential features of climate change. Thus, the demand for novel products, space, and facilities would also enhance with the increase in the population. This signifies that with the enhancement in the facilities, the environment would get influenced, which would eventually influence the climate.  

There would be more emission of carbon dioxide and thus more pollution would develop with the increase in the population of human beings, this further would add up in climate change and global warming. Thus, there is a need in the control of the human population, however, technology is also important in helping humans to sustain a better life. This technology should be advanced and well-equipped so that our climate and environment exhibits no deleterious effects.  

Novel technologies like the use of renewable energy, green building, prudent application of resources, and others would also assist the humans in sustaining the environment.  

As Hite and Seitz (2016) discuss in Chapter 5: Climate Change, reputable scientific studies continue to show that the Earth is warming at an unsustainable rate because of a rise in the release of CO2 and methane gases. What is the relationship between population increases and climate change

Answers

Answer:

See the answer below

Explanation:

The relationship between climate change and increased population of humans is a direct one. As the population of humans increases, their activities leading to the release of greenhouse gases such as CO2 and methane also increases. The greenhouse gases are primarily responsible for the warming of the earth.

As more and more greenhouse gases are released into the atmosphere, more radiation from the sun is trapped in the atmosphere and the earth receives more warmth. This extra heat received leads to increase in the global temperature, a phenomenon known as global warming. As the average global temperature increases, climate becomes more erratic and unpredictable.

The greenhouse gases-related activities of humans that are bound to increase with the increase in population include deforestation, fossil fuel burning, industrialization, etc.

Which substance is a solid?

This chart shows descriptions of the atoms for four

different substances.

Descriptions of Four Substances

Object 1 Object 2 Object 3 Object 4

Slide past Are very Form a crystal Spread out to

each other viscous

fill their

container

Object 1

Object 2

Object 3

Object 4

Answers

This question is incomplete and options are not clear; here, is the complete and correct question:

Which substance is a solid?  

A.Object 1

B. Object 2

C.Object 3

D. Object 4

This chart shows descriptions of the atoms for four different substances:

Object- Description of Four Substances

Object 1- Slide past each other

Object 2-Are very viscous

Object 3-Form a crystal

Object 4- Spread out to fill their container

The correct answer to this question is C. Object 3- Form a crystal

Explanation:

The formation of crystals or crystallization is a process that occurs only in solids because in these particles are extremely organized and placed together, which forms defined structures or crystals. Additionally, due to crystallization and the organization of particles, solids always have a defined shape and volume, which means they do not adapt to a container. Moreover, particles only vibrate but do not move freely. Thus, the fact the substance form a crystal shows this is a solid.

Answer:

c

Explanation:

Which of the following statements indicates the way in which the body's natural defenses protect the skin from the effects of UV damage?

Answers

Answer:

Prolonged exposure to the sun induces melanin dispersion, which in turn acts as a natural sunscreen

Explanation:

The statement that indicates the way in which the body's natural defense protect the skin from UV damage is "Prolonged exposure to the sun induces melanin dispersion, which in turn acts as a natural sunscreen." The correct option is D.

What are the effects of UV rays on skin?

Psoriasis can be caused by UV rays emitted by the sun or contrived references such as sunbeds.

UV rays can lead to premature skin ageing process and sun damage symptoms such as age spots, bristly skin, liver spots, actinic keratosis, and solar elastosis. UV rays could also harm the eyes.

Ultraviolet radiation has an impact on both humans and wildlife. UV-B radiation hinders the cellular activities including almost all green plants.

There is concern that ozone depletion will result in the extinction of plant species and a reduction in global food supply.

Thus, the correct option is D.

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Your question seems incomplete. The probable missing options may be:

A)The skin is protected by increasing the number of epidermal dendritic cells, which help to activate the immune system.B)Carotene, which accumulates in the stratum corneum and hypodermal adipose tissue, is synthesized in large amounts in the presence of sunlight.C)The skin is protected by the synthesis of three pigments that contribute to the skin's color.D)Prolonged exposure to the sun induces melanin dispersion, which in turn acts as a natural sunscreen.

Huntington's disease, which causes degeneration of neurons, runs in Mark's family. Huntington's is a heritable autosomal dominant genetic disease, in an autosomal dominant disease, only one abnormal allele is required to cause the disease in offspring. However, it is possible not all offspring will receive the abnormal allele from an affected heterozygous parent.
Family members that have the disease include Mark's maternal grandfather and Mark's maternal aunts. Mark's maternal grandmother does not have the disease.
Because symptoms of Huntington's disease do not appear until later in life, genetic testing is necessary to determine whether a younger individual possesses the allele for the disease. These tests determined that Mark's mother does not carry the allele for Huntington's disease.
Mark's paternal aunt suggested that Mark create a pedigree to track the disease in his family. Use the symbols provided to complete the pedigree. Each symbol may be used more than once.

Answers

Answer:

As it is given that Mark's paternal grandmother and grandfather does not carry the disease. The symbols on the right side of the Punnett represent Mark's paternal family. If both his paternal grandparents are unaffected then his father and paternal aunt are also not affected by the disease.

His maternal grandfather is affected as mentioned and also his maternal aunts are affected. Mark's mother is unaffected as she does not have the disease-causing alleles determined by genetic testing.

Both of the marks parents are unaffected which indicates that marks also do not carry the allele of the disease.

Huntington's disease is an autosomal dominant disorder in which the presence of even one dominant allele can cause the disorder.

The autosomal disorder can not skip a generation, have to be present in every generation.

Condition Given:

Affected Member: Mark's Maternal grandfather and Maternal aunts.

Normal: Mark's Maternal grandmother and Mark's Mother.

As MArk's Maternal Grandfather is affected then his genotype must be Aa because Mark's Maternal grandmother is unaffected (aa), this is the only condition where Mark's mother will be normal.

A- Dominant (Huntington)

a- Recessive

Maternal means on the mother's side and paternal means on the father's side.

The diagram is attached below.

I-1: Mark's maternal Grandmother

I-2: Mark's maternal Grandfather

I-3&4: Mark's Paternal Grandparents

II-1&2: Mark's Maternal aunts

II-5: Mark's paternal aunt

II-3&4: Mark's mother and father respectively will not have the disease as both father and mother of Mark are normal.

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A friend comes to you extremely agitated and says her husband beat her last night.She says she doesn't know what to do.You Can See She's Very panicky.Everything you suggest just seems to provoke more anxiety and fears.When you try to respond to one aspect of the problem.Your friend interrupt's frantically and jumps to another.Explain what can you do to help.

Answers

Let her get it all out and then try to help her . If she keeps panicking u might wanna take her to get some help

Why is DNA called a macromolecule by scientists? A.)because the nucleotide bases in DNA contain nitrogen atoms which make it large B.)because DNA is a massive polymer that contains many different parts C.)because DNA is made of one large, complex molecule of sugar D.)because purines and pyrimidines connect in DNA to make it a large molecule

Answers

Answer:

B.)because DNA is a massive polymer that contains many different parts

Explanation:

DNA is also called Deoxyribonucleic acid and it is important in knowing the genetic composition of organisms. The DNA comprises of phosphate, a sugar called deoxyribose, and four nitrogenous base which are adenine, guanine, cytosine, and thymine.

These constituents named above form a long chain which could be in millions and billions. This is why it is regarded to as a macromolecule because it is a massive polymer that contains many different parts .

Answer:

because DNA is a massive polymer that contains many different parts

Explanation:

1. How many bands did you detect in the lane with undigested plasmid? What size(s) were they? Explain each band. a. We detected there to be one band in the lane with the undigested plasmid.

Answers

Answer:

The aim is to eliminate organic matter by disintegrating it with said chemical compound

Explanation:

There are chemical compounds that clean the inorganic layers and others the organic ones.

In the case of sodium hydroxide, this hydroxide is capable of breaking down organic material by removing and removing it from surfaces.

Which activity involves a chemical change?
A. Ice melting into liquid water
B. A metal rod being bent into a ring
C. Colorless egg becoming a white solid
D. Salt dissolving in water
SUBMIT
Need help now pls!!

Answers

option C is correct........

.. Please follow me and mark as brainliest

The graph below shows the temperature of ocean water at different depths. Which part of the graph represents the thermocline? a. AB b. BC c. CD d. DE

Answers

Answer:

C

Explanation:

Generally temperature changes rapidly with the dept in aquatic bodies especially the oceans.The thin distinct or transition layer where the variation in temperature with depth is most significant is called Thermocline.

Its represents the gaps which  separate   the upper mixed layer from the lower deeper water bed below,it therefore represent the layer of mixture of the upper surface warmer water (C) below 200 meters, with the  cooler water layer(E) below,from 1000 meters check the attachment.

It is caused by variation in intensity of sunlight.The latter hits the surface water layer and therefore raises the temperature.But,because of decrease in  sunlight penetration,with  increasing  dept, the temperature of the  lower water dept decreases.

7. What purpose did the incubator serve in this experiment? What changes could you make to this experiment to find the optimal temperature for the bacteria converting the milk into yogurt?

Answers

Answer:

to provide optimum temperature..

Explanation:

The main purpose of the incubator in this experiment is to provide favorable temperature to the microorganisms. Incubator is a device which is used for rearing the useful microbes such as bacteria and fungi etc. This incubator provides a close environment where temperature of the environment is constant. These useful microbes are very sensitive to external temperature so incubator is used as a growing medium. In this experiment, milk is kept in incubator in order to convert into yogurt by the action of beneficial bacteria. Change the temperature of the incubator in order to find the optimal temperature for the bacteria converting the milk into yogurt.

What does swelling of the Lymph nodes indicate

Answers

Answer:

Swelling of the lymph nodes is a sign that they are being overworked.

Explanation:

Unnecessary waste could be building up in your lymph nodes and this can also signal that you may have an infection.

Hope this helped! :)

Uncellular eukaryotic organisms belong to?

Answers

All eukaryotic unicellular organisms belong to Protista.

Answer:

Unicellular eukaryotic organisms belong to kingdom protista.

Explanation:

Unicellular eukaryotic organisms belong to kingdom protista. They depend on other organisms for their food since they're not able to produce their own.

Hope this helps :)

The study of changes in phenotype that are not related to changes in the DNA and that may result from the interaction between the genotype and the environment:

Answers

Answer:

Epigenetics.

Explanation:

Epigenetics is the study of phenotypic changes which are heritable but not related to changes in the DNA sequence. It is influenced by several factors including the environment.

An example of epigenetics is DNA methylation: addition of methyl group to some part of the DNA that prevents that part from being expressed. Other examples include history modifications.

In summary, Epigenetics describes inheritance through other mechanisms than through the DNA sequence.

Which of the following is not a possible environmental consequence of urban sprawl?

a.

deforestation

b.

pollution

c.

loss of natural resources

d.

none of the above

Answers

The correct answer is D. None of the above

Explanation:

Urban sprawl or the expansion of human settlements such as cities has multiple negative consequences on the ecosystems. This includes deforestation because trees are usually cut to build houses and other human structures. Indeed, most threes and plants are removed for human construction. This also implies a loss of natural resources that include a wide variety of plants, animals, and even water as nearby bodies of water might be polluted. Besides, this general pollution increases in the area this includes noise pollution, water pollution and air pollution that result from human activities. Thus, all are possible consequences of urban sprawl; therefore, the correct answer is none of the above.

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Which 3 statements are true? 1) The citizens of Great Zimbabwe lived peacefully with one other. 2) The people from Great Zimbabwe were skilled stone craftspeople. 3) Great Zimbabwe had a system of social classes. 4) The ruling class lived in the royal city of Great Zimbabwe. 5) The chiefs and the common citizens lived together. ANSWERFind the scale factor of the dilation shown in the diagram. 1/2 1/3 2 3 a coin will be tossed 10 times. Find the chance that there will be exactly 2 heads among the first five tosses and exactly 4 heads among the last 5 tosses Las glandulas sudoriparas,al igual que las nefronas del rion,llevan a cabo procesos de reabsorcion y secrecion de sustancias antes de eliminar sudor verdadero o falso Muons are elementary particles that are formed high in the atmosphere by the interactions of cosmic rays with atomic nuclei. Muons are radioactive and have average lifetimes of about two-millionths of a second. Even though they travel at almost the speed of light, they have so far to travel through the atmosphere that very few should be detected at sea level - at least according to classical physics. Laboratory measurements, however, show that muons in great number do reach the earth's surface. What is the explanation? In cell N2, enter a formula using the IF function and a structured reference to determine if Alison Simoneau is eligible for tuition remission.a. The IF function should first determine if the staff member's Service Years 11 is greater than 1. Remember to use a structured reference to the Service Years column. b. The function should return the text Eligible if the staff member's Service Years is greater than 1. c. The function should return the text Not Eligible if the staff member's Service Years is not greater than 1. The views expressed in the passage best reflect which of the following trends in European Christendom? A rivalry for supremacy between competing sects An effort to resolve internal disputes over doctrine An indifferent attitude toward a threat to a peripheral region A willingness to unify against an opposing ideology Refer to the passage. "We will build transports to carry four thousand five hundred horses, and nine thousand squires, and ships for four thousand five hundred knights, and twenty thousand sergeants of foot. And we will agree also to purvey food for these horses and people during nine months. This is what we undertake to do at the least, on condition that you pay us for each horse four marks, and for each man two marks. And . . . we undertake to keep, wheresoever we may be, for a year, reckoning from the day on which we sail from the port of Venice in the service of God and of Christendom. Now the sum total of the expenses above named amounts to 85,000 marks. And this will we do moreover. For the love of God, we will add to the fleet fifty armed galleys on condition that, so long as we act in company, of all conquests in land or money, whether at sea or on dry ground, we shall have the half, and you the other half. Now consult together to see if you, on your parts, can accept and fulfil these covenants. Geoffrey de Villehardouin, knight and historian, Chronicle of the Fourth Crusade and the Conquest of Constantinople Suppose in 2012 in New Zealand, there are deflationary pressures and prices drop by 20 percent throughout the economy. All else being equal, expectations of increasing deflation should: solve 2x - 1/5 + 2x - 19.6 A teacher writes the name of each of her 25 students on a slip of paper and places the papers in a box. To call on a student, she draws a slip of paper form the box. Each paper is equally likely to be drawn, and the papers are replaced in the box after each draw. If there are 9 students in the last row, what is the probability of calling on a student in the last row A professional football prospect runs 40 yards dash in 5 seconds. What is the player's average speed over this distance Quadrilateral ABCD is similiar to quadrilateral EFGH. The lengths of the three longest sides in quadrilateral ABCD are 24 feet, 32 feet, and 48 feet long. If the two shortest sides of quadrilateral EFGH are 9 feet long and 18 feet long, how long is the 2nd longest side on quadrilateral EFGH? Find a solution to the linear equation y=12x24 can someone please help me Which type of worker would most likely need a bachelors degree?a plumbera carpentera construction managera construction laborer you are riding the bus to school and you realize it is taking longer because of all the stops you are making What is the solution of the following system 5x+2y+z=4 x+2z=4 2x+y-z=-1 Please answer this question now in two minutes A trolley going down an inclined plane has an acceleration of 2cm/s^2 What will be its velocity3s after the start. need help with this question, i dont think its hard for you.