You have a 5M stock solution of NaCl (Formula Weight: 58.44g/mole), a 0.25M stock solution of glucose (Formula Weight; 180.156g/mole), and a bottle of solid Tris base (Formula Weight: 121.1g/mole). How would prepare (be specific) 250mL of a single solution containing 150mM Tris, 25mM glucose, and 150mM NaCl. g

Answers

Answer 1

Answer:

4.54g of Tris base,25mL of the 0.25M stock solution of glucose and 7.5mL of the 5M stock solution of NaCl must be added and complete the volume in a volumetric flask to 250.0mL

Explanation:

To prepare the single solution we need to find the moles of each solute (Tris, glucose and NaCl) from the stock solutions anf the solid:

Moles Tris:

0.250L *(0.150mol / L) = 0.0375moles Tris * (121.1g/mol) = 4.54g of Tris base must be added

Moles glucose:

0.250L * (0.025mol/L) = 6.25x10⁻³mol glucose * (1L / 0.25mol) = 0.025L = 25mL of the 0.25M stock solution of glucose must be added

Moles NaCl:

0.250L * (0.150mol / L) = 0.0375mol NaCl * (1L / 5mol) = 0.0075L =

7.5mL of the 5M stock solution of NaCl

You must add:

4.54g of Tris base,25mL of the 0.25M stock solution of glucose and 7.5mL of the 5M stock solution of NaCl must be added and complete the volume in a volumetric flask to 250.0mL


Related Questions

Can someone answer this question please. Is this a physical change or chemical change?

Answers

Answer:

Chemical

Explanation:

the acid is a chemical and the molecules are getting torn apart by the acid

Intermolecular forces exist between what?

Answers

Answer:

Intramolecular forces are the forces that hold atoms together within a molecule. Intermolecular forces are forces that exist between molecules.

Explanation:


9. How does WNS negatively affect bats?

Answers

Answer:

Scientists hypothesized that WNS, caused by the fungus Pseudogymnoascus destructans, makes bats die by increasing the amount of energy they use during winter hibernation. Bats must carefully ration their energy supply during this time to survive without eating until spring

Explanation:

A white light shined onto a green brick bricks science

Answers

Answer:what’s the question?

Explanation:

Sorry I don’t understand Whats the question?

6. The human arm, a bat's wing, a whale's flipper, and a horse's
foreleg are examples of
A. Analogous Structures
B. Homologous Structures
C. Vestigial Structures
D. Fossils
I

Answers

Analogus Structures is the answer

Which part of the landscape shown in this image is the steepest?

Answers

Answer: A I believe

Explanation:

help! help! plz Why do two H atams combine to Form the molecule H2 while He remains monotonic?​

Answers

How atoms interact with other atoms is largely dependant on the number of electrons on its outermost layer. The electrons on this layer are called valence electrons.

As you might know, atoms have their electrons arranged in layers/shells, starting at k, l m, n etc. Each layer has a maximum number of electrons it can accommodate, with the outermost one being the valence shell. The first layer (k) can accomodate 2 electrons, the second one (l) can accomodate 8, as can the third one (m) etc.

As a rule of thumb, most atoms don't like being alone. These atoms have a valence shell that isn't saturated. This is the case for hydrogen(1), with one free slot in its outter shell.

However, atoms like helium are a bit different. They are inert, and don't need to bond with other atoms. This is because their valence shell is saturated. In this case, helium saturated the k shell with 2 electrons. Helium in this form will not form bonds.

However, hydrogen is still out there looking for buddies. Hydrogen will bind with any other atom with a free electron. Being extremely common, it's likely that said atom will be another hydrogen.

However, another common example would be for 2 hydrogens to bond with one oxygen, forming h2O (oxygen can form 2 bonds)

Conclusion : helium is inert and will not interact with other atoms. Hydrogen is not, and thus needs to bond with other atoms.

three molecules of oxygen react with four molecules of hydrogen to produce water molecules write a balanced chemical equation

Answers

Answer:

ExpC

H

4

+  

2

O

2

 

 

C

O

2

+  

2

H

2

O

This is the balanced reaction equation for the combustion of methane.

Explanation:

The Law of Conservation of Mass basically states that matter can neither be created nor destroyed. As such, we must be able to show this in our chemical reaction equations.

If you look at the equation above, you'll see an arrow that separates the reaction equation into two parts. This represents the direction of the reaction.

To the left of the arrow, we have our reactants.

To the right of the arrow, we have our products.

The quantity of each individual element in the left must equal the quantity of each individual element in the right.

So if you look below, you'll see the unbalanced equation, and I'll try to explain how to balance the reaction.

C

H

4

+  

O

2

 

 

C

O

2

+  

H

2

O

Our reactants in this equation are  

C

H

4

and  

O

2

.

Our next step is to break these down into individual atoms.

We have:

1 C atom, 4 H atoms & 2 O atoms.

If you're confused by this, look to see the little number to the bottom right of each element, the subscript, and it tells you how many of each atom are in the molecule. Make sense?

Now we look to the other side of the equation.

Here we see our products are  

C

O

2

+  

H

2

O

Again, we break these down into individual atoms again.

We have:

1 C atom, 2 H atom, 3 O atom

A reactive metal that burns with oxygen and makes a white bright light is?
sodium
iron
magnesium
copper

Answers

Answer:

it is magnesium hope it helps

The scientific method
A.is a recipe for doing science
B.will always give the right answer
C.is a systematic approach to the study of phenomena
D.involves preconceived ideas

Answers

Answer:

A. Is a recipe for soing science

Explanation:

A scientific method is a procedure for conducting science experiments, its almost like a recipe for a science experiment.

Why is nitro group called an ambident group?

Answers

Answer:

An ambident ("both teeth") group is a group that can attach to another by either of two atoms. A nitro group can bond through either N or O. In organic chemistry, this property gives rise to functional group isomers.

Explanation:

Explanation:

An ambident ("both teeth") group is a group that can attach to another by either of two atoms. A nitro group can bond through either N or O. In organic chemistry, this property gives rise to functional group isomers.

Hope this is fine for you☺️☺️☺️

Perform each conversion
5.88 dL to liters

Answers

Answer:

5.88 dL = 0.588 litre

Explanation:

We need to find a conversion from 5.88 dL to liters.

We know that the conversion is as follows :

1 deciliter = 0.1 litre

To find 5.88 dL to liters, we use the unitary method. So,

5.88 dL= (0.1×5.88) litre

5.88 dL = 0.588 litre

So, there are 0.588 liters in 5.88 dL.

(Al = 27.0 g, O = 16.0 g, H = 1.0 g)
2 Al(OH)3 Al2O3 + 3 H2O
how many grams are produced from .85 moles of AI(OH)3

Answers

Answer:

Explanation:

21

what is the answer?!!?
Plzzzz help

Answers

Answer:

The planet would stop

Good luck!

The planet will stop I agree

I need help please on chemistry

Answers

The answer should be (B) 4

2-Methyl-2-pentanol can be made starting from two different ketone electrophiles using two different Grignard reagents: one from a lower molecular mass Grignard reagent and one from a higher molecular mass Grignard reagent. Provide the retrosynthetic analysis for both routes using bromine as the halogen.

Answers

Answer:

Explanation:

In this case, we can do this by thinking a little.

Both ways use a grignard reagent, the difference between both ways is that one use an electrophile ketone with a low molecular mass, and the other has a high molecular mass.

The grignard reagent is commonly used to reduce carbonyle groups to alcohols. In the first step, a complex with the reagent is formed in the carbonile, and in the second step, the oxygen atom is hidrated in acid or basic medium and form the respective alcohol.

For the first way, we will use a high molecular mass ketone. In this case the 2-pentanone reacting with CH₃MgBr as a grignard reagent.

For the second way, we will use a low molecular mass ketone, in this case Acetone, reacting with CH₃CH₂CH₂MgBr. Both of them, will give the same product of 2 methyl-2-pentanol. See picture below for that

Hope this helps

Select the correct answer
Which hand is negatively charged?


Answers

Option B is the correct answer .

What is Charge ?

Charge is a basic property of matter that is associated with the presence or absence of electrons. Objects can have positive or negative charge, or they can be neutral, which means they have an equal number of positive and negative charges. In physics, charge is one of the fundamental concepts used to explain the behavior of matter and energy in the universe. The interaction between charges is described by Coulomb's law, which states that the force between two charges is proportional to the product of their charges and inversely proportional to the square of the distance between them.

To learn more about the Charge , click the given link ; https://brainly.com/question/25922783

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