Early genetic manipulation of petunias included an attempt to engineer dark purple petals. To this end, extra copies of the gene that causes purple pigment was engineered to be produced in the petunia. However, to the surprise of many, the plants carrying the extra copies of the purple gene were completely white or only had patches of color. Molecular analysis revealed that the purple gene was reduced 50-fold in the engineered plants compared to wild type. Provide a possible explanation how this occurred. Also, provide a possible experiment that may overcome this setback
Answer:
The altered pigmentation in transgenic petunia plants is caused by the RNA interference (RNAi) mechanism
Explanation:
The RNA interference mechanism is a naturally occurring mechanism by which single-stranded RNA molecules can bind to complementary mRNAs in order to inhibit gene expression, blocking translation and leading to mRNA degradation. The example above described is a classical genetic experiment (Napoli et al. 1990 ) that showed the existence of the RNAi pathway by the overexpression of a chimeric chalcone synthase (CHS) gene in pigmented petunia flowers, which blocked anthocyanin biosynthesis. In this experiment, irregularly bicolored/white flowers in transgenic petunias containing exogenous pigmentation genes were developed. The co‑suppression of gene expression was produced by the high-copy-number transcription of the CHS transgene, showing the existence of an undescribed RNAi silencing mechanism. Somatic reversion of the altered phenotype can be achieved by restoring the normal mRNA levels for these pigmentation genes, e.g., by a progeny testing in order to eliminate transgenic CHS expression.
Dr. Nachman and his colleagues collected rock pocket mice across 35 kilometers of the Arizona Sonoran Desert, which included both dark, rocky lava outcrops and light, rocky, granite areas. They recorded substrate color, and coat-color frequencies for each location. Each site was separated from any of the others by at least eight kilometers. The researchers trapped a total of 275 mice. Their data are summarized below (numbers have been slightly modified for the purposes of this worksheet).
Field Data Summary
Collecting Site Substrate Color Number of Mice Phenotype Light Dark
1 Dark 22 3 19
2 Light 80 74 6
3 Light 22 19 3
4 Dark 28 3 25
5 Light 58 25 33
6 Dark 65 9 56
1. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on light-colored substrates. [to 3 decimal places]
2. Calculate the overall frequencies of light-colored mice and dark-colored mice caught on dark-colored substrates. (to 3 decimal places)
3. Using the Hardy-Weinberg equation and data from the table above, determine the frequency of the three genotypes on the light, rocky, granite substrate. For genotype frequencies use three decimal places and for calculations using the allele frequencies, you must round the allele frequencies to two decimal places.
4. Using the Hardy-Weinberg equation and data from the table above, determine the frequency of the three genotypes on the dark, rocky lava substrate. (to 3 decimals). For genotype frequencies use three decimal places and for calculations using the allele frequencies, you must round the allele frequencies to two decimal places.
5. Which fur color seems to have the greatest overall selective advantage? Use data collected from both dark-colored and light- colored substrates to support your answer.
6.What environmental change gave a selective advantage for one coat color over another?
7. In a separate study, 76 rock pocket mice were collected from four different, widely separated areas of dark lava rock. One collecting site was in Arizona. The other three were in New Mexico. Dr. Nachman and colleagues observed no significant differences in the color of the rocks in the four locations sampled. However, the dark-colored mice from the three New Mexico locations were slightly darker than the dark-colored mice from the Arizona population. The entire Mc1r gene was sequenced in all 76 of the mice collected. The mutations responsible for the dark fur color in the Arizona mice were absent from the three different populations of New Mexico mice. No Mc1r mutations were associated with dark fur color in the New Mexico populations. These findings suggest that adaptive dark coloration has occurred at least twice in the rock pocket mouse and that these similar phenotypic changes have different genetic bases. How does this study support the concept that natural selection is not random?
8. To determine if the rock pocket mouse population is evolving, explain why it is necessary to collect fur color frequency data over a period of many years.
Answer:
1)
frequencies of light-colored mice ≅ 0.74 frequencies of dark-colored mice ≅ 0.262)
frequencies of light-colored mice ≅ 0.13 frequencies of dark-colored mice ≅ 0.873)
p² ≅ 0.02q² ≅ 0.742pq ≅ 0.244)
q² = 0.13 p² = 0.4 2pq = 0.465) The dark-colored fur seems to have the greatest overall selective advantage
6) Dark lava changed the color of the substrate, from light to dark.
7) Because to produce dark color, animals from the different regions suffered different mutations that drove them to have almost the same dark fur color.
8) To see if the mice population is evolving. If it is, there will be a change in the allelic and genotypic frequencies over the years. If it is not evolving, the frequencies will keep equal through the years.
Explanation:
Due to technical problems, you will find the complete explanation in the attached files
help me please
proteins are large polymers that are used for many functions, list three
What does polar ice do to much of the incoming sunlight?
Answer:
Arctic sea ice keeps the polar regions cool and helps moderate global climate. Sea ice has a bright surface; 80 percent of the sunlight that strikes it is reflected back into space. As sea ice melts in the summer, it exposes the dark ocean surface.
Explanation:
Which word describes organisms in the Kingdom of bacteria
A. Multicellular
B. Prokaryote
C. Unicellular
D. Eukaryote
Answer:
d) eukaryote
Explanation:
all fungi are eukaryotic in nature
Gina and her friends postponed their trip to the movies
because a thunderstorm was brewing, Gina helped her
mother with the laundry, Christopher decided to follow
the updates about the thunderstorm on his computer,
Rodney took a shower, and Pablo read a book in his
room. Which person would likely be the most safe from the
thunderstorm?
O Gina
O Christopher
Rodney
0 Pablo
Answer:
Pablo i think
Explanation:
edg2021 i dk if its right though
Answer:
im pretty sure its pablo <3
also, HAPPY PRIDE MONTH!!i hope your month goes fantastic
What process puts new gene segments into the genes of a corn plant to produce a new variety of corn that is resistant to disease?
O adapting
O cloning
O genetic engineering
O selective breeding
Answer:
selective breeding............
Genetic engineering allows the modification in the DNA of an organism. To obtain a disease free plant, genetic engineering is carried out. Thus, option c is the correct answer.
What is genetic engineering?Genetic engineering is a tool used in biotechnology to bring modifications or alterations in the genetic material - DNA of an organism. It modifies the DNA through addition of a gene segment or deletion of a gene.
In genetic engineering process, a specific gene of interest or the desired genetic sequence is selected. It then inserted into a cloning vector to allow its replication in the host gene. The so called 'recombinant' then formed is inserted into the host through various insertion techniques.
Genetic engineering thus allows the selection of a disease free plant, pest resistance or temperature resistant plant. A new variety of the plant can be obtained through genetic engineering.
The genome of an organism can thus be edited where the unhealthy genes can be eliminated through genetic engineering.
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An unknown bacterium has been inoculated onto a Mannitol Salt Agar plate and incubated at 37 degrees C. After 48 hours, you observe the plate. What interpretations and conclusions can be made from this result
Explanation:
Halotolerant is the ability of the organism to grow at the higher concentration of salt than the required concentration for its growth.
As we know Mannitol salt is used as selective medium for isolation of pathogenic bacteria. The medium has mannitol salt, phenol red indicator, 7.5% sodium chloride. This high salt concentration inhibits the growth of most bacteria.
The interpretations and the conclusion regarding to the given situation should be explained below.
What is Halotolerant?It is the capability of the organism for growing at a higher concentration of salt as compared to the concentration with respect to the growth. Since Mannitol salt should be applied as the selective medium for isolation of pathogenic bacteria. The medium contained the mannitol salt, phenol red indicator, 7.5% sodium chloride.
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what type of symbiotic relationship is between the anémone and the clow fish?
A.Commensalism
B.Paratism
C.Predation
D.Mutualism
The answer is D. Mutualism
what's the function of the lungs
Answer:
correct
Explanation:
Your lungs are part of the respiratory system, a group of organs and tissues that work together to help you breathe. The respiratory system's main job is to move fresh air into your body while removing waste gases.
All stories must have words.
A. True
B. False
Answer: False
Explanation: Because a story can be told with actions or signals.
Answer:
False
Explanation:
Stories can be told with an object or a series of photos not exclusively words. I hope that this helps :)
When fruits or vegetables are left exposed to the air after they have been boiled, they don't turn brown. How can you explain this?
Answer:
I guess cuz u didn't use direct heat with fire, just hot water
Explanation:
The English moth, Biston betularia, is often cited as an example of observed evolution. There are two colors of this moth, light and
dark (typica and carbonaria). Kettlewell found that dark moths constituted less than 2% of the population prior to 1848. Then, the
frequency of the dark coloration began to increase. By 1898, the 95% of the moths in Manchester and other highly industrialized
areas were of the dark color. Using the moth example, analyze the events in order to identify support for the statement: natural
selection changes populations, not individuals. Choose ALL that apply.
A)
Variation in the population existed.
B)
Color variation is a result of different gene combinations.
C)
In response to environmental change, moth coloration changed from light
to dark.
D)
Due to natural selection, the ratio of different genetic combinations is
changing
E)
Predator pressure resulted in the light colored genotype being removed
from the gene pool.
Answer:
A,B,C,F
Explanation:
A, B, C and D all of these statements support the idea that natural selection changes populations, not individuals.
What is natural selection?Natural selection is a process by which organisms that are better adapted to their environment are more likely to survive and reproduce, passing on their advantageous traits to their offspring. This leads to changes in the genetic makeup of a population over time, as advantageous traits become more common and less advantageous traits become less common.
Natural selection is driven by environmental pressures, such as predators, competition for resources, or changes in climate. It is a key mechanism of evolution, and helps to explain the diversity of life on Earth and the ways in which different species have adapted to their environments.
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Whare does digestion of starch begins?
Answer:
Mouth
Explanation:
The saliva supplies moisture, carbohydrate-digesting enzyme and amylase.
What the consequences would be if the entire water surface was covered with water lilies
Answer: There can be a ton of damage done if water lilies overgrow.
Explanation:
Water lilies protect the organisms under the body of water and provide shade to the plants and organisms underneath. These are very important for organisms, as they also provide a place to rest. But if the entire water surface were to be covered with water lilies, this would be a major problem, because sunlight would have no way of reaching below the surface, and this would decrease oxygen levels for plants and organisms. Without air and sunlight, plants will start to die and organisms of the body of water will also start to die because of no air to breathe. Also, overgrowth of water lilies causes water stagnation and leads to excessive algae blooms. The stagnant water attracts insects like mosquitoes, cockroaches, termites, and more pests which can harm plants and organisms.
5000+1000+500+50 what is its answer
Answer:
6550
Explanation:
That is the sum and you can also use a calculator to find the sum
What is the importance of living systems?
What temperature system is used by most of the world
Answer:
The temp system used by most of the world is Celcius
Answer:
The metric system, including Celsius, is the official system of measurement for almost all countries in the world. Most scientific fields measure temperature using the Celsius scale. Zero degrees Celsius is the freezing point of water, and 100 degrees Celsius is the boiling point of water.
Explanation:
Signals from nearby cells on how to differentiate are examples of an:
internal cue
external cue
Answer:
External Cue..........
Answer:
I just want points
Explanation:
A phycologist is interested in testing the claim that the proportion of algae from a local rivulet that belonged to the particular phylum cyanobacteria is at least half. A random sample of 50 alga was obtained and each alga was categorized as either being cyanobacteria or not. It was found that 38 were, in fact, cyanobacteria. The test statistic for this hypothesis test is...
Answer:
The test statistic for this hypothesis test is - 3.68.
Explanation:
A test statistic is a random variable that is calculated from sample data and used in a hypothesis test. You can use test statistics to determine whether to reject the null hypothesis. The test statistic compares your data with what is expected under the null hypothesis.
Sample proportion = 38/50
= 0.76
Hence,
Test statistic
[tex]= \frac{0.76-0.50}{\sqrt{0.50*0.50/50}}[/tex]
= 3.68
Which observation best explains how the landform in the image was created ! PLEASE HELP !!!!!!!
Answer:
dreams have been formed through the process of erosion
Explanation:
because it looks like little water streams cutting through the rock so it must have been eroded
Mode of Action of the Herbicide DCMU When chloroplasts are treated with 3-(3,4- dichlorophenyl)-1,1-dimethylurea (DCMU, or diuron), a potent herbicide, O2 evolution and photophosphorylation cease. Oxygen evolution, but not photophosphorylation, can be restored by addition of an external electron acceptor, or Hill reagent. How does DCMU act as a weed killer
Answer:
It acts as a weed killer by blocking photosynthesis
Explanation:
Photosynthesis is important to plants because it is their principal form of energy.
When DCMU is introduced to weeds, what it does is to block or slow down the process of photosynthesis. Carbon dioxide fixation is slowed down causing weeds to die off. DCMU strongly slows down photosystem II. When this is done, lipid and protein membranes would get destroyed and the weed cells would begin to dry and shed off
PLEASE HELP PLEASE!!!!!
why is it difficult for scientists to study the mechanism of evolution
Answer:
Because evolution is not a simple process and it is hard to accurately determine how a species evolved or what the species evolved from.
what are some of the structures inside a cell that help it to live and perform its role in an organism?
Answer:
hat are some of the structures inside a cell that help it to live and perform its role in an organism? mitochondria - makes ATP, lysome - digest bad things, nucleus - contains DNA ribosomes - synthesize proteins 2. ... Plant cells have a large central vacuoles, animal cells do not.
Explanation:
While they aren't spiders, Grandaddy Long legs, Horseshoe Crabs, and
Scorpions all belong to this group of organisms. *
Insecta
Crustacea
Arachnida
Nemertea
wait i may be dum but it sounds like your stating something rather then asking.. could you maybe reword the question?
What genetic trait allows organisms to survive natural selection in their
environments?
Answer:
Adaptations
Explanation:
Plzzz help I’m sooo confusseeddd
Answer:
Explanation:
allows more people to be president
Give some 4 common treatment for cAncer?????
[tex]\huge{\textbf{\textsf{{\color{pink}{An}}{\red{sw}}{\orange{er}} {\color{yellow}{:}}}}}[/tex]
Surgery, radiation, chemotherapy and hormone therapy can all be used to relieve symptoms. Other medications may relieve symptoms such as pain and shortness of breath. Palliative treatment can be used at the same time as other treatments intended to cure your cancer.
thankshope it helpsWhich of the following increases the amount of water in the atmosphere
Answer:
Transpiration
Explanation:
This is the process of water evaporating from plants into the atmosphere.
function of nervous tissue