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Why does DNA dissolve in water?



Why is salt added to solution of DNA and water?



How does ethanol help the precipitate form?



After a pellet is formed, is the DNA in the pellet or the supernate?

Answers

Answer 1

Explanation:

Because of this, DNA and RNA can easily dissolve in water. ... Adding ethanol helps the Na+ and PO3- ions come together, because ions travel easier in ethanol than water. The supernate is removed and new ethanol is added in the second washing. This removes any residual salt that remained on the pelleted DNA.

Answer 2

Answer:

DNA is polar due to its highly charged phosphate backbone. Its polarity makes it water-soluble (water is polar) according to the principle "like dissolves like". ... This fact makes water a very good solvent for charged compounds like salts.

Your DNA's sugar phosphate backbone is charged. By adding salt, we help neutralize the DNA charge and make the molecule less hydrophilic, meaning it becomes less soluble in water. The salt also helps to remove proteins that are bound to the DNA and to keep the proteins dissolved in the water.

It is well known that Ethanol has a lower dielectric constant than water, making it to promote ionic bond formations between the Na+ (from the salt) and the PO3- (from the DNA backbone), further, causing the DNA to precipitate.

Explanation:

Ideally DNA needs to be precipitated with pellet and should not remain in supernatant. DNA is acidic in nature and therefore, needs optimum salt concentration in the buffer to be pelleted from a solution. At very low salt concentration or without salt DNA would remain in supernatant.


Related Questions

What are capacitors used for

Answers

its a basic storage device used to store electrical charges and release it as it is required by the circuit. they are also used as smoothing, filtering and bypassing

Why is a “spark” of energy required to begin the chemical reaction of burning a fossil fuel? What is another name for this spark of energy?

Answers

Answer: a burst

Explanation: a burst of energy could be substatuded for spark of energy

A spark of energy is required to begin the chemical reaction of burning a fossil fuel because; This spark of energy is required to attain the activation energy of the reaction.

Another name for this spark of energy is Ignition.

Definition:

The activation energy of a chemical reaction is the minimum amount of energy required for the commencement of the chemical reaction.

In this case, the spark of energy is required to attain the activation energy of the reaction.

Read more:

https://brainly.com/question/7639475

Pro
∆G0=-RT in k Known as​

Answers

Answer:

Gibbs free energy equation

Explanation:

What is a solute?
A material that is dissolved in another material.
O A material in which another material is dissolved.
An area of space surrounding the nucleus.
O Chemical formulas on the left side of an equation.

Answers

A material that is dissolved in another material.

Which of the following is not a required characteristic for a living organism?
A) reproduction
B) mobility
C) homeostasis
D) adaptation

Answers

Answer:

B

Explanation:

living things are not required to move

The answer is C
Because it needs everything else, but u gotta choose one

Decide which element probably has a boiling point most and least similar to the boiling point of aluminum.
Comparing boiling point:
gallium rubidium magnesium oxygen
most similar to aluminum
least similar to aluminum

Answers

Answer:

The boiling point of gallium would be most similar to aluminum, and the boiling point of oxygen would be least similar to aluminum.

Explanation:

The atomic number of aluminum is 13, in the periodic table, magnesium lies in a similar period as Aluminum and Gallium is also present in a similar group. Thus, it can be expected that both magnesium and gallium exhibits similar boiling points with aluminum. However, gallium is a poor non-metal just like aluminum, while magnesium is a metal. Thus, it can be said that the boiling point of aluminum is more similar to gallium.

Now of the given elements, oxygen is a gas, thus, the boiling point of oxygen would be least similar to aluminum.

What's the electron configuration of an N-3 ion?
Question 17 options:

A)

[Ne]

B)

[Ar]

C)

[H]

D)

[He]

Answers

Answer:

A. since Nitrogen has 7 electrons and when it gains 3 electrons it will have 10 electrons. using short hand rule it is [Ne]

Humans have 50 pairs of chromosomes true or false

Answers

Answer:

False

Explanation:

humans have 23 pairs

What is electron affinity? ​

Answers

Answer:

The ability of an atom to accept an electron.

Explanation:

Electron affinity is measured by observing the energy change of a substance when an electron is added to it in its neutral gas form. For example, elements that are on the far right of the periodic table (excluding noble gases) are more likely to accept electrons rather than give them up. This is why Fluorine has the highest electron affinity of all atoms on the periodic table.

How many atoms are in 2.37 moles of Krypton

Answers

Answer:

1.43 × 10²⁴ atoms Kr

General Formulas and Concepts:

Chemistry - Atomic Structure

Using Dimensional AnalysisAvogadro's Number - 6.022 × 10²³ atoms, molecules, formula units, etc.

Explanation:

Step 1: Define

2.37 mol Kr

Step 2: Convert

[tex]2.37 \ mol \ Kr(\frac{6.022 \cdot 10^{23} \ atoms \ Kr}{1 \ mol \ Kr} )[/tex] = 1.42721 × 10²⁴ atoms Kr

Step 3: Check

We are given 3 sig figs. Follow sig fig rules and round.

1.42721 × 10²⁴ atoms Kr ≈ 1.43 × 10²⁴ atoms Kr

Help me please this is due today

Answers

The only thing I can remember from chem that pertains to this is that a neutral solution will have a pH of 7.
Also, I’m pretty sure acidity & pH have to do something with hydrogens ions or something. Sorry, hope this helps at least a little.

The list identifies various properties of four elements:

Element 1 is a gas at room temperature.
Element 2 is a solid which conducts electricity.
Element 3 is a gas with an effective nuclear charge of +7.
Element 4 is malleable and can take the form of a shiny solid sheet.

Based on this list, which elements are metals?

A. Elements 1 and 3
B. Elements 1 and 4
C. Elements 2 and 3
D. Elements 2 and 4

Answers

Answer: D

Explanation: I guessed it and got it right

A fundamental object that is difficult to divide into smaller bits is known as an element. Therefore option D is correct.

What is element ?

An element is a species of atoms, including the pure material made entirely of that species, that have a specific number of protons in their nuclei. Chemical elements, in contrast to chemical compounds, cannot be reduced by any chemical process into simpler molecules.

An element is a material that cannot be broken down by non-nuclear processes in chemistry and physics. A discrete component of a larger system or collection is referred to as an element in computers and mathematics.

Metals, nonmetals, and metalloids are the Periodic Table's three main groupings. Each group's constituent elements share comparable physical and chemical characteristics. Among the physical characteristics used to separate the three groups are, A substance's capacity to reflect light is referred to as luster.

Thus, option D is correct.

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which of the following is an isotope of hydrogen​

Answers

Z=1 is the formula i would have to see the following lol

c. What might have happened in Kush and Egypt if Kush had developed iron
weapons?

Answers

Answer:

Kush would have been able to fight better and probably would have never been invaded by Egypt, and they could have beaten the Assyrians when they tried to takeover Egypt. And the 25th dynasty would have continued.

Explanation:

that is my explanation and answer^

Percent yield is very important in terms of money. The higher the percent yield, the more of a product you can make. If you are working for a drug company and your theoretical yield is 120 kg, but you actually made 105 kg, what is your percent yield?
A. 8.75%
B. 114%
C. 87.50%
D. 15.00%

Answers

Answer:

87.5 %

Explanation:

Step 1: Given data

Theoretical yield: 120 kgReal yield: 105 kg

Step 2: Calculate the percent yield

When working with chemical reactions, we can determine the efficience in obtaining a product measuring the percent yield. The theoretical yield is the maximum amount of product that we could obtain according to the stoichiometry, whereas the real yield is the amount of product that we actually obtain when we carry out the process. We can calculate the percent yield usign the following expression.

%yield = real yield / theoretical yield × 100%

%yield = 105 kg / 120 kg × 100%

%yield = 87.5 %

Electron transfer between redox centers in proteins is controlled by quantum tunneling. We can model the region between two redox centers as an energy barrier which the electron must cross. If the distance between the redox centers is 0.681 nm and the energy of the electron is 0.5540.554eV lower than the height of the barrier, what is the probability that the electron will successfully cross to the next redox center

Answers

Answer:

5.61 e⁻³

Explanation:

Given that:

The barrier length = 0.681 nm = 6.81 × 10⁻¹⁰

The difference between the height of the barrier & the energy of the electron is;

[tex]V - E = 0.554 \ eV (1.6 \times 10^{-19} )[/tex]

= 8.864 × 10⁻²⁰ V

where;

m = 9.1 × 10⁻³¹ kg

The probability[tex]P = e^{-2 \ kd}[/tex]

[tex]k = 2 \pi \sqrt{\dfrac{2m (V-E)}{h^2} }[/tex]

[tex]k = 2 \pi \times \dfrac{\sqrt{2 \times 9.1 \times 10^{-31} \times 8.864 \times 10^{-20}}}{6.63 \times 10^{-34}}[/tex]

k = 3.806 × 10⁹

[tex]P = e^{-2 \ kd}[/tex]

[tex]P = e^{2 \times 3.806 \times 10^9 \times 6.81 \times 10^{-10}}[/tex]

P = 0.005606

P = 5.61 × 10⁻³

P = 5.61 e⁻³

Suppose you used 0.5 M NaOH to titrate your vinegar sample instead of 0.1 M. What effect does the concentration of base added have on the reliability of the results of your titration?

Answers

Answer: the reliability will be worse

Explanation:

Suppose we used 0.5 M NaOH to titrate our vinegar sample instead of 0.1 M.

Now by using 0.5M instead of 0.1M we are increasing the concentration of NaOH,

We know that  the moles used = Volume x concnetration.

so for the same no of moles, if the concentration increases, the volume decreases.

Hence it will consume less NaOH.

now Since the volume decreases, the titration volume of less number will increase the % error.

Therefore the reliability will be worse.      

The effect that the concentration of the added base has on the reliability of the results of the titration in question is; The reliability will be worse.

We are told that;

0.5 M NaOH is to be used to titrate a vinegar sample instead of 0.1 M.

Now, 0.5 M means a concentration of 0.5 and since it is higher than 0.1M, it means the concentration of NaOH will increase.

Formula for number of moles used is;

n = V × M

Where;

n is number of moles

V is volume

M is concentration

We can see that number of moles is directly proportional to the volume and concentration.

Thus, if the number of moles remains unchanged, as we have increased the concentration, it means that the volume will decrease.

Thus, lesser volume of NaOH will be used and as a result of this decrease in volume, there will be a greater possibility of error.

In conclusion, we can say that the reliability will be worse.

Read more at;https://brainly.com/question/22702792

Which scenario is likely to result in the most diverse offspring?

1 point
Sexual reproduction between two individuals from different continents
Sexual reproduction between two individuals in the same population
Asexual reproduction by binary fission
Asexual reproduction by budding

Answers

Sexual reproduction between two individuals from different continents is the answer. The other 3 offsprings wouldn’t be as diverse

Answer:   i think it is binary fission

 

Explanation:

A compound is an example of pure substance

True

False

Answers

The answers to that is true!!!

What is the degree of oxidation of a simple substance​

Answers

The oxidation state of a free element (uncombined element) is zero. For a simple (monoatomic) ion, the oxidation state is equal to the net charge on the ion. For example, Cl– has an oxidation state of -1. When present in most compounds, hydrogen has an oxidation state of +1 and oxygen an oxidation state of −2.

Answer:

The oxidation state of a free element (uncombined element) is zero. For a simple (monoatomic) ion, the oxidation state is equal to the net charge on the ion. For example, Cl– has an oxidation state of -1. When present in most compounds, hydrogen has an oxidation state of +1 and oxygen an oxidation state of -2

Calculate the molar mass of Ga(NO3)3
255.732 g/mol
159.738 g/mol
227.718 g/mol
131.727 g/mol

Answers

Answer:

255.732 g/mol

Explanation:

Molar mass of Ga(NO3)3 = 255.7322 g/mol. This compound is also known as Gallium(III) Nitrate.

From acetic acid how to get formic acid ?

Answers

Answer:

The Reaction of acetic acid withNaN3 gives out methylamine. rxn of methylamine to HNO2 to give out methanol. oxidation of methanol via Kcr207(H+) to give formic acid.

Which is stronger, the strong force (SF) or the electromagnetic force (EMF)? Describe how they battle each other in the nucleus. What would happen if their strengths were reversed?

Answers

Answer:uclear Force that holds together the nucleus of an atom. electromagnetic force. ... They are unstable because the Strong Force that would hold them together if the protons and neutrons were closer is weakened because the protons and neutrons get too far apart.

Explanation:

What do you have to do to break a molecule?

Answers

Answer:

Heat the molecule

Explanation:

Since most bonds require energy to form, they also give off energy when they are broken. But before most bonds break, the molecule has to be heated. Then the atoms start to move, and when they move too much, the bond breaks. Molecules that require less energy to break than they give off when broken are called fuels.

How high of a Frequency would a photon have to be for it to have an energy of 4 joules

Answers

The frequency of photon=6.03 x 10³³ /s

Further explanation

Radiation energy is absorbed by photons

The energy in one photon can be formulated as

[tex]\large{\boxed{\bold{E\:=\:h\:.\:f}}}[/tex]

Where

h = Planck's constant (6,626.10⁻³⁴ Js)

f = Frequency of electromagnetic waves

Energy of photon = 4 J

So the frequency :

[tex]\tt f=\dfrac{E}{h}\\\\f=\dfrac{4}{6.626.10^{-34}}\\\\f=6.03.10^{33}/s[/tex]

15 points! Answer only if you can please!
1. Have you ever grabbed the handle of a hot metal pan? Why did it feel hot to you?

2. What would happen if a person who is wearing a heavy winter jacket were to place a thermometer inside the jacket next to his or her skin? What would happen if we took the same jacket, after it had been hanging in a closet, and placed a thermometer inside?

3. How do you think the transfer of thermal energy causes unpopped popcorn kernels to pop?

Answers

Answer:

1. Yes

2. it would be the same i guess

3. because it travels to popcorn kernels with its heat and makes it pop'

did i get it right?

Explanation:

At what temperature is water a gas

Answers

Answer:

212 degrees Fahrenheit

Explanation:

When liquid water reaches a low enough temperature, it freezes and becomes a solid—ice. When solid water is exposed to enough heat, it will melt and return to a liquid. As that liquid water is further heated, it evaporates and becomes a gas—water vapor.

Explain how atoms organize to create larger structures?

Answers

Answer:

First of all they are super tiny particles of neutrons, electrons and protons. Then they come together to organize a structure of atom. Atoms come together to form a molecule and smaller molecules work together to form macromolecules. Thus, how they organize and form building blocks of a substance.

Hope it helps!<3

This item is used for small cuts and peeling.
1-Chef Knife
2-Pizza Cutter
3-Kitchen Shears/Scissors
4-Paring Knife

Answers

I would say #3 I’m sorry if it’s wrong tho
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