Answer: N-terminal green fluorescent protein (GFP)
Explanation:
Protein synthesis takes place in ribosomes located in the cell cytoplasm, in which amino acids are transported by transfer RNA corresponding to each amino acid to the messenger RNA where they bind in the appropriate position to form new proteins. The messenger RNA has a sequence of nucleotides that are translated into protein, as each codon (set of three amino acids) codes for one amino acid.
Genes are the storage units of genetic information, so they are segments of DNA that contain the information on how the cells of the organism should function. Each gene codes for a protein, so if a gene is damaged or absent, the protein will not be obtained. In this case, mutant cells with a deleted gene, will round up and wont form their normal appendages. This mutation can be rescued or repaired with a gene that encodes an N-terminal green fluorescent protein (GFP) fusion of the protein. The N-terminal end is the amino-terminal end and it refers to the end of a protein that ends with an amino acid that has a free amino group. The C-terminus or carboxyl-terminus is the end of a protein that ends in a carboxyl group. The convention for writing peptides is to place the C-terminal end to the right and write the sequence from the N- to the C-terminal end. So, when an N-terminal green fluorescent protein (GFP) fusion of the protein is expressed, the gene will produce the protein with a GFP tag.
GFP is Green Fluorescent Protein, and it is a protein produced by the jellyfish Aequorea victoria that emits bioluminescence in the green region of the visible spectrum. When a gene is fused to another gene (at either the N- or C-terminus, although in this case it is the N-terminus), the entire messenger RNA is translated together as if it were a single fused protein. Thereby, since the protein will be produce with a GFP tag, it can be seen under the microscope and it will be apropiate to use in studying cellular localization and activity.
There are some considerations that this problem does not question, such as that there must be a start codon for protein synthesis and a stop codon, and this stop codon must not be in the middle of the gene or between the gene and the GFP tag.
Migrating fibroblasts can be treated with various chemical agents while they are migrating. Explain what you expect for each of the following conditions.
a. The cells are injected with large amounts of purified gelsolin, and the live cells are observed periodically.
b. The cells are treated with latrunculin A, the drug is washed out, and the live cells are observed periodically.
c. The cells are treated with a chemical fixative and prepared for electron microscopy. Their actin filaments are decorated with myosin S1 subfragments, and you examine in which direction the arrowhead-shaped decorations are pointing in the filopodia of the fixed cells.
Answer:
c
Explanation:
how is carbon captured and stored by organisms? how is carbon passed through the ecosystem? how is carbon returned to the ecosystem?
Explanation:
i dont.know im in that qt
_____are commonly called producers
Answer:
Autotrophs
Explination: They are called this because they use the energy and simple inorganic compounds to produce organic molecules.
During which cell division phase does the nuclear membrane disappear?
A) telophase
B) metaphase
C) anaphase
D) prophase
Answer:
B)Metaphase
Explanation:
During metaphase, the nuclear membrane disappears and the chromosomes become aligned half way between the centrioles.
Answer:
Prophase
Explanation:
D
PLEASE HELP !! ILL GIVE 40 POINTS ; PLUS BRAINLIEST !! DONT SKIP ANSWER.
Answer: yo what grade is u in for these people to give you advanced word like this and also the letters are too small for me to see so I can't solve it right away.
Explanation:
Answer:
B
Explanation:
Is there a human carrying capacity on Earth? Explain why or why not.
Answer: Every species has a carrying capacity, even humans. However, it is very difficult for ecologists to calculate human carrying capacity. Humans are a complex species. We do not reproduce, consume resources, and interact with our living environment uniformly.
HOPE THIS HELPS
in our body, what is the process of moving eliminating waste?
Answer:
Explanation:
Excretion is the process of removing wastes from the body. Organs of the excretory system include the kidneys, large intestine, skin, and lungs
please help me it’s due in 20 minutes
Answer:
ok
Explanation:
1. p
2. n
3. n
4. p
5. n
6. p
7.p
8. n
Certain prehistoric events led to the formation of oil. Which of the following was one of those events? A giant wind storms destroy forests. B glaciers melted at the north and south poles . C Life in the sea died and was buried under sediment. D heavy flooring soaked the earths crust.
Answer:
C oil is just dead animal remains
Explanation:
What are the causes of soil compaction? (Site 1)
Answer:
Internal factors include particle-size distribution, organic matter content, mineralogy and water content of the soil. Externally applied forces are generally mechanically applied forces, the primary source of these being vehicular traffic
Explanation:
can you heat alcohol over an open flame. Give reason for your answer
Answer:
No, you cannot. The problem is that alcohol fumes are flammable. If you heat over an open flame you risk igniting the fumes which will start a chain reactions.
Explanation:
ggl and knowledge
20. Process through which pollutants get increasingly more concentrated each step up the food chain.
bloaccumulation
risk assessment
toxicity
biomagnification
Answer:
bioaccumulation of chemicals is the problem concerning all living organisms. This is the result of dynamic equilibrium between the uptake and elimination. The extent to which bioaccumulation occurs determines the toxic effects which are manifested. In some cases, the mechanisms of protection from certain undesirable substances deposited in special sites, to prevent their participation in the metabolic reactions, have evolved. The knowledge on bioaccumulation enables to assess the risk related with the presence of various chemicals in the environment, food, and at workplace and to present quantitatively the ability to control the use and emissions of chemicals.
Identify structure H (1 point)
מ ס ס ס וח
nou
F
-G
H
1
-K
L
Cell 2
Cell 1
Nucleus
O Cytoplasm
O Central Vaculole
Endoplasmic Reticulum
Answer:
(H) is the central vacuole!
Hope this helps ❤️
write one paragraph explaining the steps of photosynthesis
Answer:
trading a lemonade stand for an orange juice stand!!! get a taste of ur own medicine HA
Explanation:
what is the difference between sex chromosomes found in humna females and human males?
Answer:
Females have XX sex chromosomes while males have XY sex chromosomes.
Which cell division phase is shown in the image?
A) telophase
B) metaphase
C) prophase
D) anaphase
Answer:
metaphase is the correct answer of this
Answer:
b is correct
Explanation:
ok ok ok ok ok ❤️❤️
3.Some plants have structures or features that protect them from harm.
If a plant has bitter-tasting leaves, what would this most likely protect the plant against?Lector inmersivo
(1 Punto)
caterpillars that would eat the plant
cold weather that would cause the plant to freeze
people who would pick the plant for its pretty flowers
hot weather that would cause the leaves to dry up
Answer:
Caterpillars that would eat the plant
Explanation:
A prospective groom, who is not affected by cystic fibrosis (CF), has a sister with cystic fibrosis, an autosomal recessive disease. Their parents are also not affected. The brother plans to marry a woman who has no history of CF in her family. What is the probability that they will have a child with CF? They are both Caucasian and the overall frequency of CF in the Caucasian population is 1/2500 - that is, 1 affected child per 2500 (assume the population meets the Hardy-Weinberg assumptions).
Answer: In punnett square number 1, we see the cross between AA and aa, and the result is 100% Aa individuals, so there is 0% chances of haveing a child with CF. In punnett square number 2, we see the cross between Aa and aa and there is 50% chances of having a child with CF (aa genotype).
p = 0.98
q = 0.02
p² = 0.9604
q² = 0.0004
2pq = 0.0392.
Explanation:
Due to technical difficulties, the explanation has been attached as a word document.
In corn plants, the allele for tall stalks (T) is dominant over the allele for short stalks (t). Suppose a cross between a tall corn plant and a short corn plant produces 43 tall offspring and 47 short offspring. If one of the tall offspring is crossed with one of the short offspring, what ratio of genotypes would be most likely in their offspring
Complete question:
In corn plants, the allele for tall stalks (T) is dominant over the allele for short stalks (t). Suppose a cross between a tall corn plant and a short corn plant produces 43 tall offspring and 47 short offspring. If one of the tall offspring is crossed with one of the short offspring, what ratio of genotypes would be most likely in their offspring
A. 1 TT : 2 Tt : 1 tt
B. 3 Tt : 1 tt
C. All Tt
D. 1 Tt : 1 tt
Answer:
The offspring genotype ratio would be 1 Tt : 1 tt (Option D)
Explanation:
Due to technical problems, you will find the complete explanation in the attached files.
Which of the following statements correctly describes the relationship between erosion and deposition?
A.
Erosion breaks rocks into pieces, and deposition moves the pieces.
B.
Deposition and erosion both involve the movement of small pieces of rock from one place to another.
C.
Erosion is the movement of small pieces of rock from one place to another, and deposition is when the rock settles in a new location.
D.
Deposition and erosion break rocks into smaller pieces, but the rocks remain in place.
Answer:
I think It's A
Explanation:
I hope I helped :)
TRUE or FALSE
Visible light has very short wavelengths.
If FALSE explain:
PLEASE HELP!!!
Thx
4) What factors influence the circulation of the Earth's atmosphere,
thereby producing our weather?
The uneven heating of the earth's surface is one of the main variables affecting the circulation of the atmosphere globally along with seasonal variations in precipitation and temperature and Earth turning on its axis.
What is weather?The state of the atmosphere is referred to as the weather, and can be described in a number of different ways, including the degree to which it is hot or cold, wet or dry, quiet or stormy, clear or cloudy. The troposphere, the lowest layer of the Earth's atmosphere, is where the majority of meteorological events take place. The stratosphere is the next layer up from the troposphere. The term "weather" is used to describe the temperature, precipitation, and other characteristics of the atmosphere on a day-to-day basis.
Variations in air pressure, temperature, and moisture from one location to another are the primary contributors to a day's weather. These disparities can be attributed to the fact that the angle at which the Sun is positioned at any given location varies with latitude. The vast temperature difference between the polar and tropical air masses is what gives rise to the biggest scale atmospheric circulations.
Learn more about weather, here:
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[15 POINTS!!]
The diagram below shows a food web.
How would the removal of the rabbit population affect the other populations in the ecosystem?
A. The field mouse population will decrease.
B. The snake population will increase
C. The lizard population will increase.
D. The tree population will decrease
Answer:
C. because the grasshopper will have less competition to eat Grass letting them reproduce more, since the grasshopper's population increases there will be more food for the lizards helping them reproduce more.
Plsss help got to submitted today
Answer:
One interaction is between the deer and the grass the deer eats the grass and energy is transferred and then the mountain lions eats the deer and the deer's energy is transferred to the mountain lion
Explanation:
hears your answer :D
Select any true statements regarding conifers and forests. Check All That Apply Most conifer seeds can survive subzero temperatures but will be destroyed by very high temperatures. Most conifer seeds can survive subzero temperatures but will be destroyed by very high temperatures. Reforestation of a conifer forest after a wildfire can take many decades because new conifer seeds have to be brought from other areas by animals or wind.Reforestation of a conifer forest after a wildfire can take many decades because new conifer seeds have to be brought from other areas by animals or wind. Three years after a wildfire, a conifer forest will have many seedlings of the same age. Three years after a wildfire, a conifer forest will have many seedlings of the same age. Some species of conifer require fire before fertilization of their seeds can take place. Some species of conifer require fire before fertilization of their seeds can take place.
Answer:
The following are true statements regarding to conifers and forests:
O. Most conifer seeds can survive subzero temperatures but will be destroyed by very high temperatures.
O. Reforestation of a conifer forest after a wildfire can take many decades because new conifer seeds have to be brought from other areas by animals or wind.
O. Some species of conifer require fire before fertilization of their seeds can take place.
Explanation:
The true statements regarding conifers and forests include the following:
Most conifer seeds can survive subzero temperatures but will be destroyed by very high temperatures- Most conifers when exposed to heat become destroyed as their viability becomes greatly reduced but can survive very low temperatures as it doesn't affect their viability.Reforestation of a conifer forest after a wildfire can take many decades because new conifer seeds have to be brought from other areas by animals or wind- This is true as most conifers method of dispersal are through wind, animals, water etc.Some species of conifer require fire before fertilization of their seeds can take place- Species such as Lodgepole pine and Banksia require fire for fertilization as their cones or fruits that are completely sealed with resin.Read more on https://brainly.com/question/10725466
Indicate whether the given structure is located in the outer, middle, or inner ear. pharngotympanic tube helicotrema stapedius
Answer:
The pharngotympanic tube is in the middle ear.The helicotrema is in the inner ear.The stapedius is in the middle ear.Explanation:
The ear has three main parts, the outer part, the middle part, and the inner part. The outer part of the ear starts in the auricle, then it continues with the auditory canal, and it finishes with the eardrum. Then we have the middle ear that is separated from the outer one with the eardrum. In this section, it is the pharngotympanic tube, also known as the Eustachian tube. It is a tube that connects the inner ear with the pharynx to regulate internal pressure.
The inner ear has the bony labyrinth and the membranous labyrinth. In this part of the ear, the mechanical signals are transformed into electrical signals. The bony labyrinth has the cochlea, the vestibule, and the semicircular canals. The helicotrema is in the cochlea, and it connects the tympanic duct with the vestibular duct.
The stapedius is a muscle located in the middle ear. Its insertion is in the stapes, a small bone that moves with the vibrations that come from the eardrum to the malleus and the stapes. The stapedius' function is to protect our hearing by reducing the vibrations that enter this part of the ear.
Each chromosome has genes that control wing shape, body color, antenna type, and eye color.
a. True
b. False
Answer:
The correct answer is - True.
Explanation:
In a simplified drosophila or fruit fly genome, Each chromosome of the homologous pair of chromosomes has the genes for the body color, wing shape, antenna type, and eye color, and all are located on the same chromosome.
So on the basis of the explanation given above it is clear that in Drosophila or fruit flies it is true that Each chromosome has genes that control wing shape, body-color, antenna type, and eye color.
CAN FISH SURVIVE ONLY IN WATER WITH DISSOLVED OXYGEN?
Answer:
Oxygen dissolves into water from two sources: the atmosphere and from plants in the water. kills fish from oxygen depletions can range from “partial” to “total”. In a partial kill the dissolved oxygen level gets low enough to suffocate sensitive species and large fish, but many small fish and hardy species survive.
Explanation:
I hope this helped you
Pls help I’m failing
Answer:
C.
Explanation:
Not A. because it didn't mention that (and it's not true)
Not B. because that wasn't mentioned (and common sense tells you it incorrect)
C. because it was mentioned (differently) and it made perfect sense
Not D. because it didn't even talk about how many letters (or sounds) there was to be true (plus look at A. above)
A rabbit population's birth rate is 200 rabbits born/year. The death rate is 150 rabbits dying/year.
Is the population of rabbits growing or shrinking? Explain by calculating the growth rate.
Answer:
It's growing
Explanation:
The growth rate is 50 rabbits per year