Primer design is a critical step for the detection of a particular sequence in a sample. Which of the following would be true of primer design?

Answers

Answer 1

The group of choices is as follow:

A. RNA polymerase are added to the PCR mix to design random primers  

B. If detecting viral infection by PCR, the primers must not be complimentary (match) to regions of human DNA  

C. Pairs of primers must be designed, one to start amplification with Taq polymerase, the other to start amplification with human polymerase  

D. Primers are optional if you want to amplify all DNA within a sample

Answer:

The correct answer is - B. If detecting viral infection by PCR, the primers must be complementary to regions of human DNA.

Explanation:

Short, single-stranded segments of DNA sequences utilize in the real-time PCR or polymerase chain reaction technique are called primers. Primers used to hybridize the region of the segment needed to be amplified.

The human polymerase enzyme is not used in the PCR reaction and requires a 3'OH group to synthesize the DNA by amplification which is possible only in the presence of primers as they have a 3'OH.


Related Questions

Describe the ways in which the bacteria cells cluster together

Answers

Answer:

There are several different morphologies that describe particular bacteria and archaea. Archaea can have a round (cocci) shape or a rod (bacilli) shape.Bacteria can also be circular, rod, or a spiral shape (spirochete).

A cross was made between two strains of plants that are agriculturally important. One strain was disease-resistant but herbicide-sensitive; the other strain was disease-sensitive but herbicide- resistant. A plant breeder crossed the two plants and then allowed the F1 generation to self- fertilize. The following data were obtained:
F1 generation: All offspring are disease-sensitive and herbicide-resistant
F2 generation: 157 disease-sensitive, herbicide-resistant
57 disease-sensitive, herbicide-sensitive
54 disease-resistant, herbicide-resistant
20 disease-resistant, herbicide-sensitive
Total: 288 Formulate a hypothesis that you think is consistent with the observed data. Test the goodness of fit between the data and your hypothesis using a chi square test. Explain what the chi square results mean.

Answers

Answer:

Hypothesis: Alleles assort independently from each other.There is not enough evidence to reject the null hypothesis.

Explanation:

Due to technical problems, you will find the complete answer and explanation in the attached file.        

Compare and Contrast the simple electric motor and the generator using a vent diagram below

Answers

Answer:

compare

Explanation:

homie we can’t see the vent diagram because there is no pic

This is looks so difficult for me. Someone explation? And answer pl?

Answers

Answer:

That is the periodic table.

Explanation:

The periodic table has elements such as, metals, non-metals, metalloids. There are more specific groups such as, transition metals and alkali metals and halogens. This periodic table of elements is divided into different groups.

why is system thinking important

Answers

Answer:

Systems thinking is a powerful approach for understanding the nature of why situations are the way they are, and how to go about improving results. Historical perspective is important in understanding the evolution of a situation and in identifying patterns of behaviors over time.

Explanation:

Which air mass ALWAYS goes to the bottom when a warm and a cold air mass meet?

Answers

Answer:

cold air mass

Explanation:

IF you know in a fire, the hot smoke rises and the cooler air sinks toward the bottom

Cold air mass will slide beneath because it is more dense and warm air will push it up.

Does the circulatory system work with the digestive system and the excretory system?

Answers

Answer:

no

Explanation:

Which best describes the process of peer review?
A. Scientists examine each other's work to make sure it is not biased
or unreliable.
O
B. Scientists prevent other scientists from seeing their experimental
data
C. Scientists allow other people to choose their control groups.
D. Scientists allow other people to draw the conclusions from their
work

Answers

Answer:

a

Explanation:

A. Scientists examine each other's work to make sure it is not biased

or unreliable.

What Describes the layer directly beneath Earths plates

Answers

Answer:

The layer below the rigid lithosphere is a zone of asphalt-like consistancy called the Asthenosphere . The asthenosphere is the part of the mantle that flows and moves the plates of the Earth. The mantle is the layer located directly under the sima. ... The mantle is composed of very hot, dense rock.

Explanation:

Answer: This layer would be called the asthenosphere.


2.
Which of the following is an example of a prototype for the concept of leadership on an athletic team?

Answers

Answer:

the star player

The process of removing wastes from the cell is known as

A.)endocytosis
B.)exocytosis
C.) pump
D.)channel

Answers

The correct answer is B expcytosis

how does the internet work

Answers

Explanation:

internet is used by everyone this can be useful sometimes. this transmits many data and media across interconnected devices. it works its way to a packet controlling the network which follows the IP ( internet protocol ) and the transport control protocol ( TCP )

(hope this helped )

Which of the following is true of cholesterol? 4 are correct.
A. it makes up part of the cell membrane structure
B. it is the precursor molecule to make sex hormones and bile
C. it is not found in animal products such as beef
D. it enters the cell via cell-mediated endocytosis
E. it is not found in dairy products such as butter
F.it is made by the liver

Answers

Answer:

F, it is made by the liver

Explanation:

A single-celled zygote develops into a multicellular organism with specialized cells through the processes of

Answers

Answer:

mitosis

Explanation:

The zygote undergoes a process known as mitosis, in which it replicates its chromosomes (which carry each parent's DNA) and then divides, resulting in two identical cells. These cells are called totipotent and have the ability to develop into a new organism

Ocean waves are hitting a beach with a frequency of 0.100 Hz. Their
average wavelength is 15.0 m. What is their average speed?

Answers

Answer:

7 MPH or 11.27 KPH

Explanation:

Pls Mark Brainliest

A classic example of osmosis in the classroom involves placing an egg in water or syrup. The egg first sits in vinegar until the shell is dissolved. Then, it is placed in a glass of either tap water or syrup. The mass of the egg is measured. The results are in the chart below. Use what you know about osmosis to explain why the egg increased in mass in the distilled water but decreased in mass in the corn syrup.

Answers

Answer:

Water is hypotonic compared to the inside of the egg; there are more solutes in the egg than in the water. Because water moves down its concentration gradient, it moves from the distilled water into the egg. It will continue to move in this direction until there is an equilibrium between the inside of the egg and the surrounding water. This increases the mass of the egg.

Corn syrup is hypertonic to the inside of the egg. Again, water moves down its concentration gradient from the inside of the egg to the solution. This will decrease the mass of the egg.

The reason solutes do not move, instead of water, in these situations is because the egg shell (assuming it is like a chicken egg) does not allow solutes to pass though it. Only water is able to move through the shell.

Explanation:

Which one is right??

Answers

Answer: A

Explanation:                  

I neeed help ASAP please??!!!

Answers

The boys will not move since they are pulling at the same force. Hope this helps. C btw.

Look at the diagram. What type of cell is shown by number 4?

Answers

Answer:

we cannot see a diagram

What does the cardiovascular system do?

carries hormones for the endocrine system
transports oxygen and carbon dioxide for the respiratory system
sends nerve impulses to the brain and spinal cord
carries nutrients for the digestive system
works with the immune system to fight infection

Answers

It’s could be the second one but I’m not sure sorry

Answer:

transports oxygen and carbon dioxide for the respiratory system

Explanation:

compare the hygiene allergies and asthma?

Answers

Answer:

According to the “hygiene hypothesis,” the problem with extremely clean environments is that they fail to provide the necessary exposure to germs required to “educate” the immune system so it can learn to launch its defense responses to infectious organisms. Instead, its defense responses end up being so inadequate that they actually contribute to the development of asthma.

Explanation:

One of the many explanations for asthma being the most common chronic disease in the developed world is the “hygiene hypothesis.” This hypothesis suggests that the critical post-natal period of immune response is derailed by the extremely clean household environments often found in the developed world. In other words, the young child’s environment can be “too clean” to pose an effective challenge to a maturing immune system.

A dihybrid cross experiment is carried out on pocket mice. The traits being observed are fur color and tail length. In the F2 generation, 54 mice are dark brown with long tails, 18 mice are dark brown with short tails, 18 mice are tan with long tails, and 6 mice are tan with short tails.

Which traits are dominant?

tan fur, long tail

tan fur, short tail

dark brown fur, short tail

dark brown fur, long tail​

Answers

The answer is Dark brown fur, long tail

Answer:

dark brown fur, long tail​

Explanation:

Pls help i dont understand :(

Answers

Answer:

I think it might be D

Explanation:

I hope this helps~

Answer:

d

Explanation:

eukaryotes have membrane bound cells, while prokaryotes dont  

please answer quick! may give brainliest
What compound makes up carbohydrates?
a.
glucose
c.
amino acids
b.
nucleic acids
d.
phosphates

Answers

I believe the answer is glucose

Lemme know if you get it right :D

Answer:

a .glucose...

Explanation:

please mark as brainiliest

Help ASAP , easy 15 points and a brainly for the first one to reply , just answer the 2 questions

Answers

The first one is the 3rd option and the 2nd one is also the 3rd option

Which force requires contact?

A. The force of attraction between two masses

B. The force produced by interactions between electrically charged objects

C. The force exerted by air as an object moves through it

D. The force producers by interactions between magnetic objects

Answers

C. Is the answer to that question

Answer:

the force exerted by air as an object moves through it

Explanation:

got it right on my test.

Which feature may interrupt the sides of a composite-cone volcano?

conduits


fumaroles


craters


calderas

Answers

Answer:Famaroles

Explanation:Fumaroles: they are gaseous emissions from lavas in craters at more or less elevated temperatures. Its composition varies according to the temperature of the lavas, in such a way that it changes since the fumaroles appear until their extinction.Fumaroles: they are gaseous emissions from lavas in craters at more or less elevated temperatures. Its composition varies according to the temperature of the lavas, in such a way that it changes since the fumaroles appear until their extinction.Water flows in the form of steam continuously through the cracks of a volcano or volcanic surface because its temperature exceeds 100 °C.Fumaroles: they are gaseous emissions from lavas in craters at more or less elevated temperatures. Its composition varies according to the temperature of the lavas, in such a way that it changes since the fumaroles appear until their extinction.Water flows in the form of steam continuously through the cracks of a volcano or volcanic surface because its temperature exceeds 100 °C.Groups of fumaroles- Dry fumaroles: They are those that come off the melting lava, in the vicinity of the crater. Its temperature is higher than 500oC. They are mainly composed of sodium, potassium and sulfur dioxide and carbon dioxide.- Dry fumaroles: They are those that come off the melting lava, in the vicinity of the crater. Its temperature is higher than 500oC. They are mainly composed of sodium, potassium and sulfur dioxide and carbon dioxide.- Acid fumaroles: with temperatures between 300oC and 400oC, they are constituted by a large amount of water vapor, with hydrochloric acid and sulphurous anhydride.- Dry fumaroles: They are those that come off the melting lava, in the vicinity of the crater. Its temperature is higher than 500oC. They are mainly composed of sodium, potassium and sulfur dioxide and carbon dioxide.- Acid fumaroles: with temperatures between 300oC and 400oC, they are constituted by a large amount of water vapor, with hydrochloric acid and sulphurous anhydride.- Alkaline fumaroles: Temperature close to 100oC, contain water vapor with hydrogen sulfide and ammonium chloride.

The feature may interrupt the sides of a composite-cone volcano is fumaroles. Thus, option B is correct.

What is fumaroles?

They are gaseous emissions from lavas in craters at more or less elevated temperatures. Its composition varies according to the temperature of the lavas, in such a way that it changes since the fumaroles appear until their extinction.

Fumaroles are gaseous emissions from lavas in craters at more or less elevated temperatures. Its composition varies according to the temperature of the lavas, in such a way that it changes since the fumaroles appear until their extinction. Water flows in the form of steam continuously through the cracks of a volcano or volcanic surface because its temperature exceeds 100 °C.

Water flows in the form of steam continuously through the cracks of a volcano or volcanic surface because its temperature exceeds 100 °C. The lavas, in such a way that it changes since the fumaroles appear until their extinction.

Therefore, The feature may interrupt the sides of a composite-cone volcano is fumaroles. Thus, option B is correct.

Learn more about fumaroles here:

https://brainly.com/question/2222246

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We can conduct an experiment to find out if ants are happy.
O True
O False

Answers

the correct answer is that it is true


What characteristics make white dwarf stars different from main sequence stars?

Answers

Answer:

Eventually, a main sequence star burns through the hydrogen in its core, reaching the end of its life cycle. At this point, it leaves the main sequence. Stars smaller than a quarter the mass of the sun collapse directly into white dwarfs. White dwarfs no longer burn fusion at their center, but they still radiate heat.

What are the causes of inherited genetic variation

Answers

Answer:

Genetic variation can be caused by mutation which can create entirely new alleles in a population, random mating, random fertilization, and recombination between homologous chromosomes during meiosis which reshuffles alleles within an organism's offspring.

Explanation:

hope this helps!!

Answer:

Genetic variation can be caused by mutation (which can create entirely new alleles in a population), random mating, random fertilization, and recombination between homologous chromosomes during meiosis (which reshuffles alleles within an organism's offspring).

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