In a separate line of experiments, you are studying a protein that you suspect shuttles between the nucleus and the cytoplasm. However, when you localize a GFP-tagged fusion protein, it appears to be only in the nucleus. To test whether it shuttles, you set up a heterokaryon assay. You take a cell expressing the GFP fusion protein and partially fuse that cell with another that does not express the GFP protein. The result is a heterokaryon that has a single cytoplasm but two separate nuclei. You treat the heterokaryon with the translation inhibitor cycloheximide for several hours and then examine the localization of the GFP protein. What do you predict will be observed if the GFP protein shuttles between the nucleus and the cytoplasm

Answer:

Following are the responses to these question:

Explanation:

The heterokaryons were cells of diverse traditions or more nuclei. A heterokaryon cell with network cells (donor and receiver nuclei) sharing a common costly to treat is produced whenever a node containing GFP-tagged protein (donor cell) is merged to a cell non express the GFP-tagged proteins (recipient cell). Unless the intracellular scram nuclear exists, GFP must leave the GFP atoms, be shipped to the cytosol, and be exported to a nucleus with emission of GFP protein (recipient nucleus). It is obtaining nuclear has been beginning to release the GFP protein.

Unless the GFP protein doesn't display a nucleus costly to treat, this is not distributed across time in the metal target. By either sole dissemination through nuclear pores or receptor-mediated routes, protein yelling from nuclear to emotional exhaustion can occur. That GFP proteins are shown in a nucleus only suggests a path via a transmitter. For chloroplast, though, protein disperses via nuclear envelope. It is not the case. It should have nuclear foreign trade signals when ferrying between the nucleus and cytoplasm.

Cycloheximide is indeed a medicine that stops protein expression without elongating. For heterokaryon cells, it inhibits fresh protein synthesis. It makes visualization of a nanoparticle cell of only old pre-existing molecules (before cycloheximide diagnosis). That post GFP substance is a shuttling shielding substance that passes seen between the nucleus and the cytoplasm and the receiver nucleus. Whenever the levels of the shuttle were high, the GFP protein expression is shown both by the sender and receiver nucleus. The recipient nucleus will not be left without any schlepping protein. Thus, the donor nucleus can only be used.

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What structure must develop before a frog cab live on land?

What evidence does banded iron in rocks provide?
a. origin of life
b. presence of oxygen in the atmosphere
c. emergence of photosynthesis
d. volcanic activity

The picture shows the internal structures of a plant leaf.
Image shows an internal structures of a plant leaf.
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A. sunlight, oxygen, soil
B. oxygen, carbon dioxide, water
C. sunlight, carbon dioxide, water
D. water, xylem, soil

A penumbral shadow is cast when the light source is smaller than the object.

True
False

Excess fertilizer runoff into the ocean can lead to excess plant growth and algal bloom, and eventually lead to dead zones caused by ______
water.

PLEASE HELP ASAP!!!!!
Describe the founder effect.

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A) Populations that exhibit altruism in its members likely allowed more members of the group to survive and reproduce.
B) Only the youngest or weakest members of the population exhibit altruism in squirrels.
C) Only the male member of the population exhibit altruism in squirrels.
D) Squirrels who exhibit altruism are able to scare the predators away

help! two quick questions!

Because every normal body cell contains homologous pairs
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1. What are alleles?

2. Where do these 2 different alleles come from?

7. Which is the result of cell division in one-celled organisms?
A. repair
B. growth
C. reproduction

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While exploring a snag, a biologist discovers a single cell organism. Which statement BEST indicates the first thing the scientist should do to identify and classify the organism into the proper domain?

which organ of our body works the most?​

Water power is a clean and renewable source of energy, but has many drawbacks. Which of the statement describes how hydropower does not impact the environment?
A)
Large dams and reservoirs flood land habitats and change them to water habitats, forever changing the landscape and environment.
B)
Dams block the migration of fish.
C)
Dams trap moving sediment causing sand depletion and reservoir damage.
D)
Large dams create earthquakes, which increases the probability of dam failure and flooding.
E)
All of the above

Can someone help me with this?

A worm is shown in the picture. What kind of worm is it? a planarian a leech a fluke a tapeworm

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Choose the accurate statement(s) regarding the types of fibrous joints.
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b) In syndesmoses joints, the fibers are longer and the attached bones ate mote movable.
c) In gomphoses joints. two bones are linked by carriage
d) In sutures and gomphoses, the fibers are very short and a Sow for little or no movement
e) Sutures are immobile or only slightly mobile, fibrous joints that closely bind the bones of the skull to each other.

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Plz answer this test ASAP



The cardiovascular system is composed of two circulatory paths: pulmonary circulation and systemic circulation.
Pulmonary circulation transports deoxygenated blood from the right side of the heart to the



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B. lungs where blood picks up oxygen and returns to the left side of the heart

C. lungs where blood picks up oxygen and returns to the right side of the heart

D. lungs where the blood picks up oxygen and transports it to the tissues of the body

Tigers, sponges, and whales are members of the kingdom Animalia. These organisms are very different but are also similar in some ways. Which of the following is a characteristic used to classify these organisms as animals?

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The rate of diffusion is higher at increased temperatures,
True
False

Which word does not directly tie to the spindle apparatus?
1.nuclei
2.centromere
3.aster fibers
4.chromatid

A new homeowner purchased several hydrangea plants that were full of deep blue-
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an allele that is masked when a dominant allele is present.

mark)
4. What is the importance of using a hand lens in classification of organisms
​

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A. Prokaryotes
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C algea
D eukaryotes

10. In what type of plant tissue is mitosis the most easily observed under
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