If you wish to use RNAi to reduce the expression of a gene in an organism. What would you inject into the organism

Answers

Answer 1

Answer:

double-stranded RNA (dsRNA) homologous to the gene of interest

Explanation:

RNA interference (RNAi) is a naturally occurring mechanism used by eukaryotic cells to suppress gene expression through regulatory non-coding RNAs (e.g., small interfering RNAs, siRNAs) that are complementary to the target gene of interest. These non-coding RNAs can bind to target messenger RNAs (mRNAs) in the cytoplasm in order to trigger mRNA degradation and/or block translation. The RNAi mechanism is widely used in molecular biology laboratories in order to inhibit the expression of target genes and thus understand gene function. The RNAi mechanism can be induced by transfecting into cells long double-stranded RNA (dsRNA) molecules that are complementary to the target mRNA sequence to be degraded. RNAi is initiated by the enzyme Dicer that cleaves dsRNAs into siRNAs (19 to 25 nucleotides), which are then incorporated into an RNA-induced silencing complex (RISC) in order to suppress gene expression at the post-transcriptional level (i.e., the mRNA is degraded after transcription).

Answer 2

In order to use RNA interference (RNAi) to reduce the expression of a gene in an organism, you should inject a double-stranded RNA (dsRNA) hom-ologous to the gene into the organism.

Gene expression can be defined as the transcription of a gene into a messenger ribonucleic acid (mRNA), followed by its subsequent translation into proteins. Thus, gene expression is typically controlled on two (2) main levels and these are:

TranscriptionTranslation

RNA interference (RNAi) is also referred to as post-transcriptional gene silencing (PTGS) and it can be defined as a biological process in which the expression of a gene in an organism with respect to in which RNA molecules are sequentially reduced (suppressed) by double-stranded RNA (dsRNA), through transcriptional or translational repression.

This ultimately implies that, you should inject a double-stranded RNA (dsRNA) hom-ologous to the gene into the organism, if you wish to use RNA interference (RNAi) to reduce the expression of a gene in an organism.

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Related Questions

can increase biodiversity. (1 point)
O Changes in temperature
O Decreasing the food supply
O Limiting mates
O Protecting land

Answers

Biodiversity is the variety of life habiting the earth's ecosystem with numerous genetics and ecosystem levels. Protecting lands will increase biodiversity.

What is biodiversity?

Biodiversity is the variety of living organisms living together in an area, that works together in an ecosystem to support life and maintain the population.

Biodiversity is affected by the temperature, food, reproduction rate, and habitat. Protecting the habitats will increase biodiversity.

Changes in temperature will affect biodiversity negatively, and decreasing food and reproduction ability will decrease the population of the biodiversity.

Therefore, option D. protecting lands can increase biodiversity.

Learn more about biodiversity here:

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HELP!!!
What role do plants play in the carbon cycle?
A.
Plants release CO2 during photosynthesis, adding it to the atmosphere.
B. Plants use CO2 during photosynthesis, removing it from the atmosphere.
C. Plants use CO2 during respiration, removing it from the atmosphere.

Answers

b, plants use co2 during photosynthesis, removing it from the atmosphere

photosynthesis is 6CO2 + 6H2O → C6H12O6 + 6O2

respiration is C6H12O6 + 6O2 → 6CO2 + 6H2O

therefore, it is not released during photosynthesis or used during respiration.

Fast environmental change can be caused by and

Answers

I mean global warming is pretty fast ngl

How in the world r wolfs endangered?!!!! They hunt in packs so humans would at least need a semi-automatic riffle or something like that and before he/she kills all the wolfs more than likely they will be died so will give u all my points

Answers

Answer:

Humans are a big reason for this issue. Things such as pollution, hunting, construction, and animal testing all effect the wolves population. Their food is hunted by humans so its harder for them to hunt, humans tear down trees and take over ground so theres less places to live, they might eat things like plastic because people litter, and things like dangerous weather can harm these animals.

Living things_______in response to changer in there environment. 1.evolve. 2.fossils. 3.extinct. 4.species. Can some tell me which one it is?

Answers

The answer is evolve

Help please.

Potatoes are specialized stems called tubers. Potato tubers have two functions. (1) These thick terms are used to store food. (2) The eyes on the stems, seen here, are used to


A) absorb water.

B) grow new plants.

C) make more sugar.

D) collect oxygen for respiration.​

Answers

Answer:

B, grow new plants

Explanation:

They are not actual eyes, new potatoes grow out from the potato "eyes".

In a good experimental design, the part of the experiment that is intentionally changed is the
A dependent variable.
B independent variable.
C constant
D control

Answers

Constants never change.

Controls typically don't change or they are done by standard means

(like in an experiment where you water plants with sodas, the plant you use water with is the control)

Dependent variables are the outcome of the experiment. This is the result of changing something.

Independent variables are what you are testing. A independent variable is something that is  intentionally altered.

easy way to remember it is that dependent variables depend on what other variables are. so you cant change a dependent variable, but you can change the other variables.

The number in each circle below represents the chromosome number of the cell. Which diagram represents the production of
skin cells?
16
రంగం
(1
(2)
(3)
(4)
1
2
OOOO
3
Od
4

Answers

Answer:

1

Explanation:

Cause the diagram represents the production of skin cells 16

Define los 5 sentidos humanos

Answers

Taste, Touch, sight, smell, sound

Question 1 Negative Forcings: increase the amount of sea ice at the north pole for instance. O increase the growing season. increases the amount of energy in the Earth's budget. decrease the amount of energy in Earth's energy budget.​

Answers

Answer:

increases the amount of energy in the Earth's budget.

Explanation:

Increase occurs in the amount of sea ice at the north pole leads to increases the amount of energy in the Earth's budget because this sea ice reflects the solar radiation back to space. Sea ice has a bright surface which causes 80 percent of the solar radiation reflected back into space. As sea ice melts in the summer, it exposes the dark ocean surface which leads the ocean to absorbs 90 percent of the sunlight which heat up the oceans, and increase in the temperature occurs causes more melting of ice so increasing sea ice is good for the earth.

Is it a good idea for humans colonize mars pls real answers :)

Answers

Answer:

I believe yes and no at the same time

Explanation:

If we do end up colonizing Mars, Earth could slowly get better as time goes on, but when we do colonize Mars, wont we just do the same thing we did to Earth?

Answer:

Yes it could be, here are the pros.. Colonizing Mars is one of the most promising backup plans for humanity . Mars is relatively close to our Earth . Colonizing Mars may become possible in just a few decades . Many celebrities support those plans . Many scientists advocate for this kind of backup plan . Extraction of additional natural resources . May help with the search for other life forms . We could learn as global human species . Humanity needs challenges to progress . Knowledge is power . May help to mitigate the overpopulation problem on Earth . Colonizing Mars is an image project . Future generations would be able to adapt quite quickly on Mars . May be a good long-term investment for institutional investors

But there are also some cons.. Colonizing Mars is extremely expensive . It takes a while to travel to Mars ; Lack of water may become a serious problem . Plenty of insecurity involved in this plan . Completely different living conditions . Humans need protective equipment before going outside . It’s freezing cold on Mars . Some people have to make the beginning . May not be justifiable from an ethical standpoint . Colonizing Mars may conflict with religion . Money can be better used to solve our problems on planet Earth . Some scientists believe that efficient live on Mars will not be possible . Quality of life on Mars would be much lower . Rather insecure food supply . Radiation may become a problem . Still unclear if living on Mars is sustainable . We would alter the balance in our universe . Colonizing Mars can be dangerous . Space exploration implies significant pollution . Other life forms may not be welcoming towards us humans . Short-term costs vs. long-term gains

In conclusion, no it would not be a good idea.

I will make the first person to answer brainliest!

Answers

Answer: The amount of water in the stream and the gradient.

However if you had two streams both with the same amount of water in them but with one stream flowing down a steeper slope (gradient) than the other, then the steep one has more ability to erode.

Explanation:

Which shows the correct order of processes for fungi to reproduce sexually

Answers

Sexual reproduction in the fungi consists of three sequential stages: plasmogamy, karyogamy, and meiosis. The diploid chromosomes are pulled apart into two daughter cells, each containing a single set of chromosomes (a haploid state). ... In most fungi the zygote is the only cell in the entire life cycle that is diploid.

Answer:

C

Explanation:

You make two agar plates: one is a nutrient agar plate (plate A) that contains histidine and penicillin. The other is a glucose salts agar (plate B) that also contains penicillin. You inoculate a sample onto both plates using replica plating technique, incubate the plates, and compare the growth after 48 hours. There are 12 colonies on the nutrient agar plate and 11 colonies on the glucose salts medium. Which of the following statements is INCORRECT?

a. The colony that is missing from plate B is an auxotroph that cannot synthesize histidine.
b. The prototrophs are resistant to penicillin but the auxotrophs are sensitive to this antibiotic.
c. Prototrophs and auxotrophs in this experimeſit are resistant to penicillin.
d. Approximately 92% of the bacteria in the original sample are prototrophs.
e. This experiment describes indirect selection of a mutant.

Answers

I’ll help u out of u can help me out with mines asap please

whati is cell division?​

Answers

Answer:

Cell division is the process by which a parent cell divides into two or more daughter cells. Cell division usually occurs as part of a larger cell cycle.

mechanics is that branch of physics and mathematics that...​

Answers

Answer:

The correct answer is - deals with the motion of an object without or with the reference of force.

Explanation:

The mechanism is the branch of sciences that deals with an object in a motion under the reference force or the object that remains at rest. This branch of physics leads to topics of gravitational force, electricity, quant, or magnetism according to the nature of the forces involved.

Modeling, aerodynamics, and all other applications of mechanics required the use of laws of newton, the formula of mathematics, and algebra.

PLEASE HELP QUICK!!!

What does mRNA do?

A. Carries copies of instructions to make proteins from the DNA to the rest of the cell

B. Creates proteins on the
ribosome

C. Transfers amino acids to the ribosome during protein synthesis

D. Carries amino acids to the growing polypeptide chain on the ribosome

Answers

A. mRNA carries copies of instruction to make proteins from the DNA to the rest of the cell.

mRNA is transcribed from DNA, then translated by the ribosomes into a polypeptide chain (protein)

: propels food through the digestive tract. : lines internal and external body surfaces. : conducts electrical impulses. : transports oxygen to the body tissues. : functions in secretion and absorption. : pumps blood. : stores fats and minerals.

Answers

Answer:

1. Muscle tissue.

2. Epithelial tissue.

3. Nervous tissue.

4. Connective tissue.

5. Epithelial tissue.

6. Muscle tissue.

7. Connective tissue.

Explanation:

A tissue can be defined as a group of cells that are structurally similar and in close proximity. Tissues are generally responsible for performing specific functions in living organisms such as humans, animals and plants. Therefore, tissues in living organisms function together as a unit.

There are four main types of tissues and these includes;

I. Connective tissue.

II. Epithelial tissue.

III. Muscle tissue.

IV. Nervous tissue.

In this scenario, the various types of tissues are matched with their functions as follows;

1. Muscle tissue: propels food through the digestive tract. It is mainly performed by peristalsis.

2. Epithelial tissue: lines internal and external body surfaces. It also referred to as epithelium and it comprises of sheets of cells.

3. Nervous tissue: conducts electrical impulses. A neural circuit can be defined as a group of neurons in the body of a living organism which are interconnected by synapses to perform specific functions when they're activated.

4. Connective tissue: transports oxygen to the body tissues predominantly through the Erythrocytes (red blood cells).

5. Epithelial tissue: functions in secretion and absorption.

6. Muscle tissue: pumps blood.

7. Connective tissue: stores fats and minerals.

when are dntps used in the polymerase chain reaction

Answers

Answer:

dATP, dTTP, dGTP and dTTP are four common dNTPs used in PCR. The function of dNTPs in PCR is to expand the growing DNA strand with the help of Taq DNA polymerase. It binds with the complementary DNA strand by hydrogen bonds. The PCR is an in vitro technique of DNA synthesis.

Explanation:

hope this helps you

Name three components of the blood

Answers

Answer:

plasma, red blood cells, white blood cells, and platelets.

Explanation:

Hope this helps!

In one page compare and contrast the processes of aerobic and anaerobic respiration.​

Answers

Aerobic respiration: takes place in the presence of oxygen.
Anaerobic respiration: takes place in the absence of oxygen.

- Carbon dioxide and water are the end products of aerobic respiration.
-Alcohol is the end product of anaerobic respiration.
- Aerobic respiration releases more energy than anaerobic respiration.

Question on the image below! Help please

Answers

1. Aa 2.aa 3. Aa 4. aa

You transform a population of cells with a transgene and isolate a cell line that has integrated the gene into its genome, but in which the gene is not expressed. Speculate as to what may be preventing the gene from being expressed. Describe two ways to test this possibility.

Answers

Answer:

The transgene could be silenced by: 1- epigenetic mechanisms (e.g., DNA methylation, histone deacetylation, etc.), and 2-the RNA interference (RNAi) pathway

Explanation:

Epigenetics refers to the heritable changes caused by the activation and deactivation of genes without changing the DNA sequence. Epigenetic mechanisms include 1-DNA methylation on cytosine residues, and 2-modifications on amino acids in the tails of histone proteins (e.g., methylation, acetylation, ribosylation, phosphorylation, etc). These two epigenetic mechanisms can modify DNA structure and thus alter the accessibility of transcription factors, thereby suppressing gene/transgene expression. Moreover, the RNA interference pathway (RNAi) is a naturally occurring mechanism capable of suppressing gene/transgene expression both at transcriptional and post-transcriptional levels. The RNAi mechanism is triggered by regulatory non-coding RNAs (ncRNAs) such as, among others, long non-coding RNAs (lncRNAs) and small ncRNAs (e.g., miRNAs, piRNAs, siRNAs, etc). In this case, for example, DNA methylation on promoter sequences could suppress transgene expression, thereby methods to measure DNA methylation can be used to test this possibility (e.g., bisulfite genomic sequencing). Moreover, miRNAs that bind to the messenger RNA of the transgene could also inhibit its expression by triggering the RNAi pathway (e.g., mRNA degradation), thereby methods to measure mRNA levels can be used to test this possibility (e.g., RT-PCR).

What's one nonmedical way that a society can slow down the spread of influenza?
antibiotics
quarantines
vaccines
antivirals

Answers

Quarantines. All other options are medical ways.

Answer:

C

Explanation:

que tipo de raices? es el cesped
zanahoria
papa
habichuela
helechos
remolocha
clasificar en raises fibrosa o primaria por favor lo necesito lo ante posible para subir la calificacion de la clase sin enlance por favor gracias

Answers

Answer: papa or remolocha

Explain how DNA supports the theory of evolution?​

Answers

Answer:

Because there are tens of thousands of genes in humans and other organisms, DNA contains a tremendous amount of information about the evolutionary history of each organism. ... It determines evolutionary relationships among organisms, and it indicates the time in the past when species started to diverge from one another.

Drag each label to the correct location.
Annie wants to study the effect of glucose on bacterial colonies. She grows two colonies of bacteria—one with glucose present in the environment and one in an environment without glucose. Categorize the statements about the experiment as either hypotheses or observations.

Glucose may cause
an increase in
bacterial growth.
The bacterial colonies grown
without glucose don’t have
capsules around their cells.
Bacterial growth in the
glucose environment
increased rapidly and
then stopped after
some time.
Increased glucose may
lead to capsule
formation in bacteria.
Reset Next

Answers

Answer:

* Glucose may cause an increase in bacterial growth - HYPOTHESIS

* The bacterial colonies grown without glucose don’t have capsules around their cells - OBSERVATION

* Bacterial growth in the glucose environment increased rapidly and then stopped after some time - OBSERVATION

* Increased glucose may lead to capsule formation in bacteria - HYPOTHESIS

Explanation:

In an experiment, a HYPOTHESIS is a predictive and testable statement that aims to answer a scientific question while an observation is made based on the results obtained.

According to this question, Annie wants to study the effect of glucose on bacterial colonies. She conducted the experiment by growing two colonies of bacteria - one with glucose present in the environment and one in an environment without glucose. Based on this experiment, the following statements are categorized as:

- Glucose may cause an increase in bacterial growth is a HYPOTHESIS

- The bacterial colonies grown without glucose don’t have capsules around their cells is an OBSERVATION

- Bacterial growth in the glucose environment increased rapidly and then stopped after some time is an OBSERVATION

- Increased glucose may lead to capsule formation in bacteria is a HYPOTHESIS

In fruit Flies a white eyed female is crossed with a red eyed male. What are the changes the offspring will be a white eyed male?​

Answers

Answer:

the chances of the offspring having white eyes is 50%

Explanation:

i used a punnet square to calculate the output:)

Answer:

25%

Explanation:

There's a 50% chance of the offspring being male & 50% chance female. The genotypes must be co-dominant otherwise their question doesn't give enough information to get an anwser. Also a 50/50 chance of red/white, so added together a 25% chance.

Also, the funny thing is that remember this from 7th grade so you must be reviewing things that evreyone learned a long time ago but forgot.

PLZ help I need the answer today!!!

Answers

Answer:

I think it's because the electricity from the battery goes through one light bulb and into the other and back to the battery since there is only one circuit and when one of the light bulbs burn out it stops the circulation of electricity causing the other one not to work too?

My laptop charger is dead and my phone charger is dead and my laptop is in my car charger I don’t really want my phone number I don’t know how to answer my phone

6. What regulatory processes would your body use if you were trapped by a blizzard in an unheated, uninsulated cabin in the woods (or in a heat wave with no air conditioning)

Answers

Answer:

Shivering and sweating.

Explanation:

A prolonged exposure to extreme cold environment would activate the brain's heat-gain center. Your brain's heat-gain center causes shivering in order to increase our muscle contraction which leads to warming of our body.  Increase sweating is the regulatory processes our body use if it experience very high temperature. In order to regulate internal temperature of body, our body remove water which takes up heat with itself to lower th internal temperature.

Other Questions
Will give brainliest:) please help with these complicated Spanish questions what is an equivalent expression for 90+45?what is an equivalent expression for 10(3 + 4)? What is the prepositional phrase in the sentence below: We walked along a trail. please help me!!! justify your answers. Find the measure of each angle indicated (quadrilaterals)I think its 100 I just wanna make sure if it is Which is greater? 2/6 or 4/10? A prism with a height of 2, width of 3 andlength of 10 can hold how many cubic inches? How can extinctions and expansions occur in the same habitat? Explain your answer. Based on the density graph below, what is the probability of a value in the sample space being anywhere from 10 to 25?A. 80% B. 60% C. 40% D. 20% I dont wanna answer without making sure so help You are writing a device driver for an audio device connected to your computer. Your code needs to determine if the device is busy or ready to accept a new command. How can the driver determine this Evaluate (-2) + (-3)2. please helppp maybe write out the equation too BRAINLIEST IF CORRECT !!!! in ABC m You have a 100 ohm resistor. How much resistance must you add in parallel to the 100 ohm resistor to create an equivalent resistance of 75.0 ohms? Please help me this due in a couple of hours and yes i do have to do them all and ill give brainly help me please i will give brainliest!this is spanish btw PLEASE HELP IF POSSIBLE :) Which of these statements are true?Select all that apply.Experimental probability is determined by trials.Theoretical probability can be any number from 0 to 1.Experimental probability can be any number from 0 to 1.Experimental probability is determined by estimating.Theoretical probability is greater than experimental probability. Read the passage.The girls walked quietly past the house on the corner hoping that Uncle Raymond would not notice them. The cousins had promised to helpUncle Raymond clean up his yard after school, but they had forgotten and gone to a club meeting instead. They remembered only when theystepped out of the building with the rest of the robotics club and saw the neat piles of leaves that now lined Uncle Raymond's yard across the streetfrom the school. They felt bad that he had done all the work himself and knew that he would be very displeased that they hadn't helped him whenthey said they would. The cousins wanted to get home without an unpleasant scene. Most of all, they wanted to avoid a talk with Uncle Raymond.Based on the events in the passage, which word would best replace the word "talk" in the last sentence?O 1. debate02. dialogueO 3. conversation4. confrontation