. g Determine the number of moles in 0.05 µg of a 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ratio of insert to vector if you have 10 ng of vector that is 2686 bp?

Answers

Answer 1

Answer:

a. 2.4 x 10^-24 mol

b. 5.4 x 10^4

Explanation:

a. Mass of DNA (of 0.05µg)= 0.05µg x number of base pairs x 650Da

=0.05x300x650Da

(1 Da=1.67x10-24g)

=9750 x 1.67 x 10^-24

=16282.5 x 10^-19µg =1.62 x10^-21 g

No. of moles of insert DNA=mass of DNA(in g)/mass of one mole in g/mol

=1.6 x 10^-21g/660 g/mol

=2.4 x 10^-24 mol

Hence, moles of the insert DNA is calculated as 2.4 x 10^-24

b.

First of all, let us determine number of moles of vector to calculate the molar ratio.

No. of moles of vector=10 x 10^-9 x 2686 x 650 x 1.67 x 10-24/660

=2.68x6.5x1.67x10-27/660

=4.4 x 10^-29 mol

Molar ratio of insert to vector=2.4 x 10^-24/4.4x10^-29

=5.4 x 10^4

So, the molar ratio of the vector and insert as per the data is calculated as 5.4 x 10^4


Related Questions


The ___ cycle is not a nutrient cycle.

Answers

Answer:

water cycle

Explanation:

The water cycle is not a nutrient cycle

Which of the following is a known limiting factor for good nutrition

Answers

Answer:  

seems you forgot the "followings" but here are some known limiting factors

Explanation:

   Exercise,Physiology, Mindset, Nutrition and Genetics.

Explain why detritivores, decomposers and omnivores are not assigned trophic level​

Answers

Answer:

They can be found in several trophic levels, as they are related with several trophic level, hence, they cannot be placed on a specific trophic level.

Explanation:

A trophic level is a specific position occupied by a group of organisms on a good chain in relation to their nutrition.

Dentrivores feed on the waste of other organisms and cannot be really placed on a specific trophic level, likewise decomposers, of which they feed and decompose the body of organisms.

Omnivores feed on both plants and animals, which includes producers and consumers. Placing omnivores on a specific trophic level would not be really straightforward also.

Why would damage to the nervous tissue in the spinal cord lead to paralysis?
Damage results in the loss of signals to muscles to produce movement.
Damage results in the inability of the body to differentiate between voluntary and involuntary movement.
Damage results in the inability of the body to produce connective tissue to support movement.
Damage results in the decreased production of actin and myosin for contraction

Answers

Answer:

B. Damage results in the inability of the body to differentiate between voluntary and involuntary movement

Explanation:

Answer:

b

Explanation:

Colistin is an antibiotic used to treat infections in the bloodstream. Some bacteria are resistant to Colistin. Explain how these bacteria have become resistant to Colistin.

Answers

Answer:

They have adapted to it

Explanation:

If it is accepted that the information for how a protein is intended to fold lies within its primary sequence, explain why a purified protein denatured in vitro might not be able to renature accurately.

Answers

Answer:

Denaturation and renaturation are properties of protein but in some cases denaturation is irreversible and protein is unable to renature.

The primary sequence of a protein determines which portion of protein fold closely together in order to form three-dimensional conformation and the conformations are held by hydrogen bonds.

Denaturation of protein in-vitro is generally obtained by increasing temperature. Heat or high temperature disrupts hydrogen bonds that held between protein folds because high temperature increases the kinetic energy and the bonds are disrupted due to rapid heating. Disruption of hydrogen bonds will change the 3D structure of the protein and denatured proteins lose its function.

Chemicals are also used to disrupt hydrogen bonds such as alcohol.

So, high temperature and chemicals denature protein by affecting the hydrogen bonds holding 3D shape of primary sequence in protein and the change in protein becomes irreversible.

The women has had two miscarriages. What is the chance that she could have a normal child? a. The chance of a normal child is approximately one in four; however, all of the normal children will be translocation carriers.b. The chance of a normal child is approximately one in two; however, all of the normal children will be translocation carriers.c. The chance of a normal child is approximately one in four; however, half of the normal children will be translocation carriers.d. The chance of a normal child is approximately one in two; however, half of the normal children will be translocation carriers

Answers

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Changes in DNA sequence from one generation to the next may result in offspring that are altered in fitness compared with their parents. The process of change and selection over the course of many generations is the basis of

Answers

Answer:

This question lack options, options are:

A. Mutation

B. Evolution

C. Heredity

D. Reproduction

The correct answer is B.

Explanation:

Evolution is essentially a process of genetic change over time, that is, it is the result of accumulated changes in the composition of the gene pool. Variation is the raw material of evolution. Without genetic variation, evolution is not possible. The ultimate source of all genetic variation is mutation. A mutation is a stable and inheritable change in genetic material. Mutations alter the DNA sequence and therefore introduce new variants. Many of these variants are usually eliminated, but occasionally some of these variants can be successful and incorporated into all individuals of the species. Mutation is a factor that increases genetic diversity. This is how evolution explains the origin of various life forms as a result of changes in their genetic load. The inheritance of these variations through offspring of parents who carry the variation, although the genetic endowment of a single organism is the same throughout life, the genetic makeup of a species, understood as a whole (genetic background), changes throughout from many periods of life. Mutations and variability, the product of natural DNA recombination, provide diversity in the genetic material of a species.

1. In a standard autoclave procedure dry heat is used combined with high pressure
A. True
B. False

2. Household bleach is routinely used in dilution 1:10
A. True.
B. False

Answers

Answer:

1) F, 2)True

Explanation:

1) autoclave uses steam

2) bleach dilute is 1:10

1. It is false that In a standard autoclave procedure dry heat is used combined with high pressure.

2.  It is true that Household bleach is routinely used in dilution 1:10

1. In a standard autoclave procedure, moist heat, not dry heat, is used in combination with high pressure. Autoclaves use steam under high pressure to achieve sterilization by destroying microorganisms and their spores. The high-pressure steam provides effective heat transfer and penetration, ensuring the sterilization of the items being processed.

2. Household bleach is often used in a dilution of 1:10, meaning one part bleach is mixed with nine parts water. This dilution is commonly recommended for various household cleaning and disinfection purposes. It helps to ensure effective disinfection while reducing the potential harmful effects of concentrated bleach.

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Please describe the regulation of the blood glucose 6 hours after a meal?
Please include all relevant organs, hormones, and actions.

Answers

Answer:

Find the description below in the explanation section

Explanation:

The body system regulates the blood sugar level via the hand-in-hand working of insulin and glucagon hormones, both produced by the Pancreas. After a meal, the food undergoes digestion and is broken down into glucose, which is absorbed into the bloodstream. Insulin hormone is released by the pancreas (stimulated by a negative feedback) when the sugar level of the blood increases. This secretion of insulin causes cells to absorb glucose as source of energy, while liver and muscle cells store some of the excess blood glucose as GLYCOGEN.

However, after about 6 hours of taking the meal, the blood sugar (glucose) level must have decreased. This triggers the secretion of Glucagon hormone by the Pancreas. Glucagon hormone, acts in an opposite manner as Insulin hormone, by causing the stored GLYCOGEN in the liver and muscle cells to be converted back to Glucose in order for cells to make use of it.

This continuous process occurs and it is how the body regulates the blood glucose levels.

-Lab-
"Why are cells so small"​

Answers

Answer:

Explanation:

Because we need a lot of cells and if they were big only a small about would fit.

How are lysosomes pro-nuclear dense bodies?

Answers

Answer:i hope this answers ur question

Explanation:

when the community climax in an ecosystem has only a single type of climax, the development of the climax community is controlled by ___ of that region.

a) the climate
b) the life forms
c) the soil texture
d) the pioneer community

Answers

Answer:

A.

Explanation:

because the climax community is composed of species beat adopted to average conditions in that area

When the community climax in an ecosystem has only a single type of climax, the development of the climax community is controlled by the climate of that region. Option A is correct.

The climate of a region is the most important factor that controls the development of a climax community. The climate determines the temperature, precipitation, and other abiotic factors that influence the growth and survival of plants and animals.

In a region with a single type of climax community, the climate is relatively uniform and there is little variation in the abiotic factors. This means that the same species of plants and animals will be able to survive and thrive in the region, regardless of the specific location. The life forms, soil texture, and pioneer community can all influence the development of a climax community, but they are not as important as the climate. Option A is correct.

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The largest taxon and consists of all the other taxis called

Answers

Answer:

Domain

Explanation:

While viewing stained onion root tip cells you notice that distinct chromosomes cannot be observed in most of the cells. This is because most of the cells Group of answer choices

Answers

Answer:

This is because must of the cell must be in INTERPHASE.

Explanation:

The onion root tip is the growing part of the onion and many cells in it undergo mitosis. In the lab, it is prepared in a way that the cells become flat on the microscope slide so that you can observed them well.

In the cell cycle, most of the onions root tip cells are in interphase, they spend most of their life in interphase. In interphase, there is no observable changes in the celks, the cells keep on growing and the nucleus is been duplicated. This is because most of the onion root cells spend most of their time reproducing and growing in the interphase stage. During the interphase, the cells can still be seen in dark spots because the nucleus is still present and prophase has not started yet.

Suppose a plant breeder wants to isolate mutants in tomatoes that are defective in DNA repair. However, this breeder does not have the expertise or equipment to study enzymes in DNA repair systems.
Which methods would best allow the breeder to identify tomato plants that are deficient in DNA repair?
A) Expose putative DNA repair mutant plants to ionizing radiation L and see if they survive less well than wild-type plants.
B) Measure the amount of post-translational processing that occurs in the putative DNA repair mutant plants. Measure the somatic mutation rates for the putative DNA repair mutant plants.
C) Expose the putative DNA repair mutant plants to a deaminating agent and select those that have a decreased mutation rate.
D) Measure the germ line spontaneous and induced mutation rates for the putative DNA repair mutant plants.

Answers

The provided question has marked options incorrectely the correct order as follows:

A) Expose putative DNA repair mutant plants to ionizing radiation L and see if they survive less well than wild-type plants.

B) Measure the amount of post-translational processing that occurs in the putative DNA repair mutant plants.

C) Measure the somatic mutation rates for the putative DNA repair mutant plants.

D) Expose the putative DNA repair mutant plants to a deaminating agent and select those that have a decreased mutation rate.

E) Measure the germline spontaneous and induced mutation rates for the putative DNA repair mutant plants.

Answer:

The correct answer is: A), C) and E)

Explanation:

The breeder wants to isolate the mutants in tomatoes that are defective in DNA repair, to identify such plants breeder should follow these methods as they will allow best to identifying the defective tomatoes-

Measure the somatic mutation rates for the putative DNA repair mutant plants.

Measure the germline spontaneous and induced mutation rates for the putative DNA repair mutant plants.

Expose putative DNA repair mutant plants to ionizing radiation L and see if they survive less well than wild-type plants.  This will allow the breeder to see and analyze the ability of DNA repair in tomatoes

A student has the high-power lens in position and appears to be intently observing the specimen. The instructor, noting a working distance of about 1 cm, knows the student isn't actually seeing the specimen. How so?

Answers

Answer:

Explanation:

Based on this the student is out if the focus, and just viewing on  the dust.Basically, high -power lens is a non oil immersion lens, with a short working distance  of 1cm,which  will be about  a few millimeters.Hence the student will be out of focus, just carry out a fruitless effort  focusing on the dust, missing the focus on the specimen.

The function of the plasma membrane is....

Answers

Answer:

to protect the cell from it's surroundings

Explanation:

the plasma membrane is selectively permeable to ions and organic molecules and regulates the movement of substances in and out of cells

Explanation:

The primary function of the plasma membrane is to protect the cell from its surroundings.

Sorry if the ans is wrong

who is Charles darwin​

Answers

Answer:

Charles Darwin came up with the theory of evolution.

Charles Darwin is a writer who wrote the book Orgin of species . He is the one that gave the idea of evolution . his proposition that all species of life have decended over time from common ancestors. He is a english naturalist, geologist and biologist

How have the fields of biotechnology, genetic engineering, and molecular biology affected the agricultural job
market?
A.They have created more jobs
B.They have spread the industry internationally
C.They have made the market obsolete
D.They have decrease the demand for farmers

Answers

I think answer should be d.let me know if it’s correct thanks okay bye

Answer:

I think It's A. Hope it helps! :)

Explanation:

Which gas is a major contributor to ocean acidification? A. oxygen B. sulfur dioxide C. carbon dioxide D. nitrogen

Answers

Answer:C, carbon dioxide.

Explanation:Carbon Dioxide reacts with water forming Carbonic acid. This acid increases the acidity of the ocean.

Answer:

Explanation: got it correct

A single point mutation in a gene results in a nonfunctional protein. Individuals heterozygous for this mutation were identified using a Southern blot. Which pair of wild-type (WT) and mutant alleles most likely contains the mutation?
A. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCGATGCTTAGGCATCT-3
B. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCGAAGCTTAGGCATAT-3
C. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTTGAAGCTTAGGCATCT-3
D. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCCAAGCTTAGGCATCT-3

Answers

Answer:

C. WT 5-TAGTCGAAGCTTAGGCATCT-3

Mutant 5-TAGTTGAAGCTTAGGCATCT-3

Explanation:

In the wild type, the DNA sequence encodes the following aminoacidic segment: SRSLGI; while the mutant form has a stop codon in the second codon (TGA). A stop codon is a trinucleotide sequence in the messenger RNA (mRNA) that indicates a stop signal in protein synthesis. There are three types of stop codons: UGA, UAG and UAA, where U represents Uracile in the mRNA instead of Thymine in the DNA sequence.

Representation:  

C. WT 5-T AGT CGA AGC TTA GGC ATC T-3 >> 5'3' Frame 1 = SRSLGI

Mutant 5-T AGT TGA AGC TTA GGC ATC T-3 >> 5'3' Frame 1 = S-, where - represents a stop codon (TGA) and therefore it is the end of the protein.

What brings greater concentrations of dissolved nutrients to the ocean surface?

Answers

Answer: Upwelling

Explanation:

Upwelling is a process of movement of dense, cold and nutrient rich water from the bottom of the ocean to the surface layers due to wind currents. This process replaces the warm and nutrient deficient water from the surface.

This process is useful for the growth of phytoplanktons and other sea plants growing in the surface layer of oceanic water.

In 1668 Francesco Redi did a series of experiments on spontaneous generation. He began by putting similar pieces of meat into eight identical jars. Four jars were left open to the air, and four were sealed. He then did the same experiment with one variation: instead of sealing four of the jars completely, he covered them with gauze (the gauze will exclude the flies while allowing the meat to be exposed to air). In both experiments, he monitored the jars and recorded whether or not maggots (young flies) appeared in the meat.
Refer to the paragraph on Redi's experiments. In both experiments, fies appeared in all of the open jars and only in the open jars. Which one of the following statements is correct?
A. The experiment was inconclusive because it did not nun long enough
B. The experiment supports the hypothesis that spontaneous generation occurs in rotting meat.
C. The expertment supports the hwpothesis that maggots arise only from eggs laid by adult flhes
D. The experiment was inconclusive because Redi used only one kind of meat

Answers

Answer:

The experiment supports the hypothesis that maggots arise only from eggs laid by adult flies; that is  only from reproduction and development.

Explanation:

The objective of this experimental set up by Francesco Radi  was to demonstrate the concept of spontaneous generation. He concluded that, Maggots are not spontaneous generated from rotten meats, rather they are products of eggs laid by houseflies who visited the rotten meat to lay eggs. The maggots (larva) are products of complete metamorphosis, from egg to larva to pupa and adult. This is an indication that, new organisms are not formed form spontaneously, but are products of well-defined process of reproduction and development which in this case is a  complete metamorphosis.

Based on this premise he concluded that, if maggots were products of  laid eggs, then they should appear only  when rotten meat is exposed to  open air.However,if they are f products of spontaneous generation they should appear irrespective of  the meat exposure to air or not.

3. Mutations that occur in the BPG binding cavity on deoxyhemoglobin can at times result in the substitution of lysine and histidine residues present with aspartic acid and phenylalanine residues. Explain how these changes would impact BPG binding to deoxyhemoglobin along with the impact it would have on oxygen transport with hemoglobin

Answers

Answer:

It affects the oxygenation of the tissues in a negative way, that is, causing tissue hypoxias.

Explanation:

What will happen is that BPG, having a different relationship with deoxyhemoglobin, hemoglobin will not give oxygen to organs or tissues during glycolysis at times when the sympathetic nervous system is activated or even in the maintenance of physiological tissue vitality, therefore it will trigger a drop in the partial pressure of the gas both in the blood and in the tissues since they will be retained in the hemoglobin structure without being able to release it.

A medical term with a suffix usually describes a/an

Answers

Answer:

a condition, an action, or a relationship.

Explanation:

5 Points

Great Britain would have a much colder climate than it does if not for the

O A. polar easterlies

B. gulf stream

C. trade winds

D. jet stream

SUBMIT

Answers

Hello There!

Answer:

B. Gulf stream

Explanation:

Great Britain would have a much colder climate than it does if not for the gulf stream.

what is a animal and what types of animals are there

Answers

Answer:

Animals are multicellular eukaryotic organisms that form the biological kingdom of Animalia. With few exceptions, animals consume organic material, breathe oxygen, are able to move, can reproduce sexually, and grow from a hollow sphere of cells, the blastula, during embryonic development.

Explanation:

Scientists have recently estimated that there are approximately 8.7 million species on Earth. They believe that 1-2 million of those species are animals.

Rutherford tracked the motion of tiny, positively charged particles shot through a thin sheet of gold foil. Some particles traveled in a straight line and some were deflected at different angles. Which statement best describes what Rutherford concluded from the motion of the particles? Some particles traveled through empty spaces between atoms and some particles were deflected by electrons. Some particles traveled through empty parts of the atom and some particles were deflected by electrons. Some particles traveled through empty spaces between atoms and some particles were deflected by small areas of high-density positive charge in atoms. Some particles traveled through empty parts of the atom and some particles were deflected by small areas of high-density positive charge in atoms.

Answers

Answer:

Some particles traveled through empty parts of the atom and some particles were deflected by small areas of high-density positive charge in atoms.

Explanation:

Rutherford concluded from his experiment that there are empty spaces present in most parts of the atom while a heavy positive charge is present in the nucleus of an atom due to which the tiny positively charged particles deflects because of positive-positive charge repulsion. Most of positively charged particles passes undeflected which indicated the presence of empty spaces in the atoms. The electrons revolve around the nucleus have no effect on the deflection of positively charged particles.

Rutherford conducted a legendary experiment to determine atomic structure in which he bombarded a thin gold foil with alpha particles. In the aftermath of the experiment he concluded that; Some particles traveled through empty parts of the atom and some particles were deflected by small areas of high-density positive charge in atoms.

The gold foil experiment showed that the atom is composed of a massive core which contains positively charged particles.

This massive core is at the center of the atom surrounded by electrons that move in orbits. Hence, the resulting model of the atom was called the planetary model.

The deflected positively charged particles were deflected by this small area of high-density positive charge in atoms.  

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The process of succession is necessary to maintain and establish stable communities. T or F

Answers

Answer: True it is necessary

Answer:

True

Explanation:

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