Fire and Mello conducted an experiment where they added no gene X RNA (b), anti-sense gene X RNA (c) or double stranded gene X RNA (d) to C. elegans embryos. They then did a hybridization experiment examining gene X mRNA levels in the embryo. Square (a) is a no probe control. How does this act as a control

Answers

Answer 1

Answer:

The correct answer is - It shows that an RNA probe is necessary to get a signal.

Explanation:

A probe is a specific single-stranded sequence of DNA or RNA that recognizes its complementary sequence in a particular sample genome for synthesis. The RNA probe is placed into close contact with the given sample under a specific environment that allows the RNA probe sequence to hybridize with its complementary sequence.

In this case, square a is the condition where no RNA probe is persent whereas all three other conditions have some sort of RNA probe, however, in C there is no Gene X probe but their probe present. To get a signal in hybridization RNA probe is required and that can be used as control as it can be used to compare if a signal is received or not.


Related Questions

3. If you were looking at a cell that had all of its chromosomes lined up down the middle of
the cell, what phase must it be in?
1. Telophase
2. Prophase
3. Metaphase
4. Anaphas

Answers

Answer:

3. Metaphase

Explanation:

Metaphase is the stage in the cell cycle where the chromosomes line up in a line down the middle of the cell (some people call it the metaphase plate).

The way I like to remember it is: Metaphase is in the Middle.

I hope this helps! :D

Answer:

I'm sorry hindi ko po alam

Chemical energy in the form of food is changed into what?

Answers

Food is the most important source of chemical energy for living things. The chemical energy in food is converted, or changed, by the body into moving mechanical energy and heat energy. People have invented ways to use the chemical energy of substances to power machines.

Scientists observed that the populations of top-level consumers in a particular ecosystem were rapidly decreasing. Further studies revealed that there was also a decline in producer productivity. Which other changes did the scientists most likely observe in the ecosystem?


increased producer diversity


decreased population size at all levels


decreased primary consumer populations only


increased primary and secondary consumer diversity

Answers

Answer:

The correct answer is - decreased population size at all levels.

Explanation:

A decrease in the productivity of producers of an ecosystem will lead to a decrease in all other organisms of the ecosystem including top-level consumers to the bottom-level producers due to the fact that producers provide energy and food to primary consumers.

It is because they are the only organisms that convert the sunlight to usable energy and food that transfer from one trophic level to other, so if the population of producers or their productivity will decrease then there will be decreased population size at all levels.

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