Describe the difference between an acute transforming virus and a virus that does not cause tumors Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Reset Help stops tumor formation An acute transforming virus is a that , whereas a that can inhibit cellular genes nonacute virus bring about tumor formation DNA virus carries an oncogene(s) can induce the activity of cellular genes retrovirus

Charles Darwin based his ideas on common ancestry and evolution on a variety of sources of evidence, including the following four:

1. Fossil Record: Fossil evidence shows that there have been changes in organisms over time. For example, the fossil record of horses shows that they have evolved from small, three-toed ancestors to large, single-toed animals over millions of years.

2. Comparative Anatomy: Comparing the anatomy of different species can reveal similarities and differences that suggest common ancestry.

For example, the similar forelimb structure in humans, bats, whales, and other mammals suggest that they share a common ancestor.

3. Comparative Embryology: The study of embryonic development in different species can reveal similarities and differences that suggest common ancestry.

For example, all vertebrate embryos pass through a stage where they have gill slits, suggesting that they share a common ancestor with fish.

4. Biogeography: The geographic distribution of species can provide evidence for evolution and common ancestry.

For example, the similarities in plant and animal species on either side of the Atlantic Ocean suggest that they share a common ancestor but were separated by the formation of the ocean.

These sources of evidence for evolution provide support for the idea of common ancestry, which suggests that all species on Earth share a common ancestor and have evolved over time through a process of natural selection.

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The traits that are farther apart on the chromosomes are brown skin and large leaves of potatoes.

If a potato has a 35% chance of passing on brown skin with few eyes and a 10% chance of passing on brown skin with large leaves, the traits that are farther apart on the chromosomes are brown skin and large leaves. Brown skin is inherited via the chromosome responsible for skin pigmentation and large leaves are determined by a separate set of genes, thus these traits are farther apart on the chromosomes. Brown skin and few eyes, on the other hand, are closely linked on the chromosome and are inherited together frequently. Therefore, these two traits are located closer together on the chromosome.On the other hand, brown skin and large leaves are considered to be located farther apart on the chromosome. The 10% chance of passing on brown skin with large leaves indicates that these traits are not inherited together as frequently as brown skin with few eyes. This suggests that the genes responsible for brown skin and large leaves are located on different regions of the chromosome and are further apart.

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Based on the data provided, it can be concluded that gene expression in the patient's cells is different from that of the other 5 patients. This can be inferred from the gene expression heatmap, which shows varying levels of expression across different genes in each patient.

The patient's cells exhibit a unique pattern of gene expression, with higher levels of expression in certain genes compared to the other patients. This difference in gene expression may be indicative of a specific disease or condition that is unique to the patient.

It may also suggest that the patient has a genetic variation or mutation that is affecting their gene expression profile. Further analysis of the patient's genetic makeup may be required to fully understand the underlying causes of this difference in gene expression.

Overall, the data suggest that gene expression is highly variable across individuals and can provide valuable insights into disease pathology and personalized treatment options. By studying gene expression patterns, researchers can better understand the mechanisms of disease and develop targeted therapies for individual patients.

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In the Kirby-Bauer method, the antibiotic that would be most effective to treat a bacterial infection is the one that is marked as Sensitive.

The Kirby-Bauer method is a laboratory technique used to determine the effectiveness of antibiotics against a particular bacterial strain. During this method, small discs impregnated with different antibiotics are placed on an agar plate inoculated with the bacteria in question. After an incubation period, the plate is examined for inhibition zones, indicating how effective the antibiotic was at preventing bacterial growth.

The results are interpreted by comparing the zone sizes to a standardized table that classifies the bacteria's response to each antibiotic as Sensitive, Resistant, or Intermediate. The Sensitive classification indicates that the antibiotic is effective against the bacterial strain being tested, while Resistant indicates that the bacteria are not affected by the antibiotic. The Intermediate classification suggests that the antibiotic may somewhat affect the bacteria but not enough to inhibit its growth completely.

Therefore, the antibiotic marked as Sensitive is the most effective one to treat bacterial infection.

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Here are the key points:

1) We do not have identified the specific gene(s) that directly code for speech production and language ability. Speech and language are complex cognitive abilities that emerge from the interaction of many genes and brain regions. They cannot be boiled down to a single gene.

2) It is difficult to develop testable hypotheses and models about the exact timing of the emergence of speech from ancient hominid fossils. Soft tissues like larynx and vocal cords do not preserve in the fossil record. There are no direct recordings or other traces of speech from fossils older than ≈100 years.

3) However, through comparative studies of modern humans and other great apes, and examination of fossil records, scientists can make inferences about the anatomical, physiological and neurological changes that likely enabled speech. Some key possibilities include:

• Development of a receding chin and modification of the larynx, enabling more complex sounds. This may have emerged around 200,000 years ago with Homo sapiens.

• Growth of brain regions involved in communication, language processing and social cognition. The cerebral cortex expanded significantly in humans, especially regions like Broca's and Wernicke's areas.

• Changes in vocal learning abilities, allowing humans to learn complex and nuanced sounds for symbolic communication. This may have enabled the emergence of syntactic speech.

• Gradual changes in torso shape, spine and breathing that improved breath control and vocal flexibility. This was likely a long process spanning several million years.

So in summary, while definitive proof is lacking, developing testable hypotheses and models about the emergence of speech is possible through comparative study and inference from available fossils and genetics. But this remains an open area of research and new evidence could continue shaping our understanding.

Does this help explain the key points? Let me know if you have any other questions!

Yes. Through comparative study and analogy, it is possible to develop testable hypotheses and models for when speech appeared.

While soft tissue does not preserve and there were no recordings before 100 years ago, we can still examine the fossil record and look for structures that support speech in modern humans.

Additionally, the gene that codes for speech has been identified, and we can sequence genomes as old as 1 million years old. By analyzing these various sources of information, researchers can develop hypotheses and models that can be tested through further research and experimentation.

While we cannot directly observe or hear ancient human speech, we can use genetic research, comparative studies, and the fossil record to develop testable hypotheses and models to estimate when speech first appeared in human evolution.

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Answer:This is called a gene-environment interaction or gene-environment interplay.

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Main Answer: The passage of an arthropod through stages from egg to adult is called metamorphosis.

Supporting Answer: Metamorphosis is a process of transformation that involves a series of developmental changes in an organism from one distinct stage to another. In arthropods such as insects, crustaceans, and spiders, metamorphosis is a complex process that includes distinct stages, including egg, larva, pupa, and adult. During metamorphosis, arthropods undergo significant morphological, physiological, and behavioral changes that allow them to adapt to different environments and lifestyles. For example, many insects undergo complete metamorphosis, in which the larval stage looks and behaves completely differently from the adult stage, with different feeding habits and body structures. This allows the adult to occupy a different ecological niche than the larva, reducing competition for resources. In contrast, arthropods that undergo incomplete metamorphosis, such as grasshoppers, undergo gradual changes in body form and function as they mature, with no pupal stage.

Therefore, the correct answer is option d) metamorphosis.

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The BEST way a polyacrylamide gel should be placed in a MiniProtean running box is: b. The wells should be at the top with the shorter glass plate away from the user.

The MiniProtean running box is designed in such a way that the wells for loading the protein samples are located at the top of the gel. The gel is composed of two glass plates with a gel in between them, and it is clamped into position using the clamps located at the bottom of the box.

The shorter glass plate is usually positioned towards the back of the running box, away from the user, while the longer glass plate is positioned towards the front, closer to the user.

When placing the polyacrylamide gel in the MiniProtean running box, the wells should be positioned at the top, with the shorter glass plate located away from the user. This ensures that the protein samples are loaded into the wells correctly, and that the electrophoresis buffer can flow through the gel and carry the proteins towards the anode at the bottom of the gel.

Additionally, the shorter glass plate should be positioned so that it touches the green rubber gasket when the gel is clamped into position. This helps to create a tight seal, which prevents the buffer from leaking out of the running box during the electrophoresis process.

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The three most abundant elements in Earth's crust are oxygen (O), silicon (Si), and aluminum (Al).

Oxygen is the most abundant element, constituting approximately 46% of the Earth's crust by mass. It is a key component of minerals such as silicates, oxides, and carbonates. Oxygen is also a vital element for life, present in water (H2O) and many organic compounds.

Silicon is the second most abundant element, making up around 28% of the Earth's crust. It is a major constituent of various minerals, particularly silicates, which form the building blocks of rocks and minerals found on the Earth's surface.

Aluminum is the third most abundant element, comprising roughly 8% of the Earth's crust. It is found primarily in minerals such as feldspars, clays, and micas. Aluminum is widely used in various industries due to its strength, lightweight nature, and resistance to corrosion.

These three elements play crucial roles in shaping the composition and structure of the Earth's crust, and their abundance influences geological processes, mineral formation, and the availability of resources for human activities.

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The most accurate statement that reflects what we think happened early on during the process of lactase persistence is that A) individuals in those populations who possessed alleles that lead to increased lactase synthesis as adults had higher fitness than individuals in those populations with other lactase alleles.

Evolutionary physiologists believe that some human populations recently evolved the ability to digest lactose as adults after they domesticated large mammals. This is known as lactase persistence. The ability to digest lactose is a complex trait that is controlled by multiple genes, but the primary gene involved is the lactase gene.

It is believed that early on during the process of lactase persistence, individuals in those populations who possessed alleles that lead to increased lactase synthesis as adults had higher fitness than individuals in those populations with other lactase alleles. This is because the ability to digest lactose provided a nutritional advantage, especially in environments where other sources of food were scarce.

As individuals in those populations started drinking the milk of their domesticated animals, expression of their existing lactase genes increased, and this change was passed on to their offspring. This process of natural selection led to the prevalence of lactase persistence in those populations.

It is not believed that lactase genes from domesticated animals were incorporated into the human genome through horizontal gene transfer. While horizontal gene transfer can occur in some organisms, it is not a common mechanism for gene transfer in humans.

In conclusion, the most accurate statement that reflects what we think happened early on during the process of lactase persistence is that individuals in those populations who possessed alleles that lead to increased lactase synthesis as adults had higher fitness than individuals in those populations with other lactase alleles. As individuals in those populations started drinking the milk of their domesticated animals, expression of their existing lactase genes increased, and this change was passed on to their offspring through natural selection.

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The given statement Heterotrophic organisms use organic compounds, usually from other organisms, as carbon sources. Autotrophic organisms use carbon dioxide (CO2) as their only source or their main source of carbon is True.

Heterotrophic organisms are unable to produce their own organic compounds from inorganic sources, and instead, obtain their carbon from pre-existing organic compounds, usually from other organisms. Autotrophic organisms, on the other hand, are capable of synthesizing their own organic compounds using simple inorganic compounds, such as carbon dioxide (CO2), as a source of carbon.

In summary, the key difference between heterotrophic and autotrophic organisms is their ability to produce their own organic compounds from inorganic sources. Heterotrophs rely on pre-existing organic compounds, while autotrophs are self-sufficient and can synthesize their own organic compounds using inorganic sources.

Hence, the given statement is true.

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The conditioner that contains a vegetable protein and is acidic, which causes the cuticle to close after alkaline chemical services, is the "acidifying conditioner" or "normalizing conditioner." Option (d) is the correct answer.

Acidifying or normalizing conditioners contain vegetable proteins, such as keratin or soy protein, that can help to strengthen the hair shaft. They are also formulated with an acidic pH, which can help to neutralize any alkaline residues left on the hair after chemical treatments such as coloring or perming.

The acidic pH of these conditioners also helps to close the hair cuticle, which can make the hair appear smoother, shinier, and less prone to tangling or breakage.

Therefore, the correct option is D.

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A cougar hunting a deer in a diorama is a realistic depiction of the predator/prey dynamic. This model uses a cougar to represent the predator and a deer to represent the victim.

The cougar actively hunts and preys upon the deer in this model, which captures the dynamic interplay between these two animals. It emphasises the part of the predator in pursuing and catching its prey. The diorama also illustrates the environment's physical features, such as the landscape and plants, which are essential to comprehending the predator-prey dynamic. Overall, by depicting the hunt and the interdependence between the two species, this model successfully depicts the essence of the predator/prey dynamics.

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Food poisoning caused by Salmonella in meats and eggs can result in the bacteria multiplying in the lumen of the gut, leading to inflammation of the gut lining and symptoms like diarrhea and abdominal cramps. In some cases, the bacteria can enter the bloodstream, which can result in more severe symptoms and complications.

The route the Salmonella bacteria take to enter the bloodstream is through the intestinal wall. Salmonella bacteria are able to penetrate the intestinal epithelium, which is the lining of the gut, by using a type of secretion system called a type III secretion system (T3SS). Once the bacteria have penetrated the epithelium, they can enter the bloodstream and spread to other parts of the body, causing systemic infections.

It is important to note that not all cases of Salmonella infection result in the bacteria entering the bloodstream. In many cases, the bacteria remain confined to the gut and are eliminated through fecal excretion. However, in cases where the bacteria do enter the bloodstream, prompt medical attention is necessary to prevent serious complications.

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The first step in a signaling pathway that responds to a molecule remaining in the extracellular space is binding of the signal molecule to a receptor. The correct option is c.

In this process, the signal molecule, also known as a ligand, does not enter the cell. Instead, it interacts with a specific receptor protein embedded in the plasma membrane. This receptor is typically a transmembrane protein with extracellular, transmembrane, and intracellular domains.

Upon binding of the ligand to the extracellular domain, the receptor undergoes a conformational change, which subsequently initiates a series of intracellular signaling events. This process is known as signal transduction. Depending on the specific signaling pathway, various proteins within the cell may become activated through mechanisms such as phosphorylation or dephosphorylation.

The activated proteins then relay the signal through a series of biochemical reactions, ultimately leading to cellular responses, which may include changes in gene expression, cell division, or other cellular functions. This mechanism allows cells to sense and respond to external signals efficiently without directly internalizing the signaling molecule, and it plays a vital role in regulating many biological processes.

Hence, the correct option is c.

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An important function of the bones in the skeleton is to support the body.

The skeletal system provides a strong and stable framework that supports our organs, muscles, and tissues. This framework enables us to stand upright, maintain posture, and perform a wide range of movements, such as walking, running, and lifting objects.

Contrary to the other options mentioned, bones do not primarily provide a source of ATP or generate hormones. ATP (adenosine triphosphate) is produced mainly through cellular respiration in cells' mitochondria, while hormones are typically generated by endocrine glands, not bones.

Although bones do have a role in producing certain hormones, such as osteocalcin, this is not their primary function.
Lastly, while bones do add weight to our body, their primary purpose is not to simply add mass. Instead, their primary purpose is to support and protect the body, as well as assist with movement by acting as levers for muscles to pull on.

In summary, the skeletal system plays a vital role in providing support to the body, which enables various physical activities and protects vital organs.

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Yes, there are several processes that can account for the persistence of deleterious alleles in a population besides deleterious recessive alleles. One such process is genetic drift, which refers to random fluctuations in the frequencies of alleles in a population due to chance events. In small populations, genetic drift can lead to the fixation of deleterious alleles, even if they are harmful to individuals carrying them.

Another process is the presence of heterozygote advantage, where individuals carrying one copy of a deleterious allele may have an advantage over both homozygotes in certain environments. This advantage can maintain the allele in the population at higher frequencies than would be expected based on its negative effects alone.

Finally, some deleterious alleles may only have negative effects later in life, after individuals have already reproduced and passed on the allele to their offspring. In these cases, the allele may persist in the population despite its harmful effects.

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The repair mechanisms responsible for maintaining the integrity of DNA include mismatch repair, recruitment of translesion polymerase, DNA recombination, and direct repair.

Mismatch repair is a system that identifies and corrects errors that occur during DNA replication, such as base mismatches or small insertions/deletions. This process helps ensure accurate copying of the genetic material, preventing mutations from arising.

Recruitment of translesion polymerase is another DNA repair mechanism, which comes into play when the replication machinery encounters damaged DNA. Translesion polymerases are specialized enzymes that can bypass DNA lesions, allowing replication to continue despite the damage. Although they can be error-prone, these polymerases help to maintain genomic stability by preventing replication forks from stalling.

DNA recombination is a process that can repair damaged DNA by exchanging genetic material between similar molecules. This mechanism is particularly important for repairing double-strand breaks, which can be lethal if left unrepaired. Recombination allows the cell to use a homologous DNA molecule as a template to accurately repair the broken DNA, preserving its integrity.

Direct repair involves enzymes that can directly reverse DNA damage, without the need for excising or replacing the damaged base. For example, the enzyme photolyase can repair UV-induced pyrimidine dimers by splitting the dimer and restoring the original bases. Direct repair is a rapid and efficient mechanism for fixing certain types of DNA damage, contributing to overall genomic stability.

These mechanisms work together to ensure the maintenance and preservation of DNA integrity, preventing the accumulation of mutations and safeguarding the genetic information within the cell.

Thus, the repair mechanisms that are responsible for maintaining the integrity of DNA are mismatch repair, recruitment of translesion polymerase, DNA recombination, and direct repair.

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The plasma membrane of skeletal muscles, which can conduct electrical signals, is also known by the term "sarcolemma."

The plasma membrane of skeletal muscles is also known as the sarcolemma. The sarcolemma is a specialized plasma membrane that covers the muscle fibers (cells) and allows for the conduction of electrical impulses, which is necessary for muscle contraction. The sarcolemma is composed of a phospholipid bilayer, which separates the interior of the cell from the extracellular fluid.

Embedded within the sarcolemma are a variety of proteins, including ion channels, receptors, and transporters, which allow the muscle cell to interact with its environment and carry out its functions.

Overall, the sarcolemma is a critical component of skeletal muscle function, allowing for the efficient transmission of electrical signals that drive muscle contraction.

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The group of non-prokaryotic organisms studied in microbiology that typically have two life cycle stages: trophozoite and cyst are protozoa. The correct option is B

Unicellular eukaryotic organisms known as protozoa can be either parasitic or free-living. They are categorized according to how they move, with amoebas, ciliates, flagellates, and sporozoans serving as some typical examples.

The protozoan is in its active, feeding, and reproducing stage during the trophozoite stage, whereas the cyst stage is a latent, resting state that enables the organism to withstand challenging circumstances like dryness, freezing, or nutrition deprivation.

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True. Ascaris is a genus of parasitic roundworms that share certain anatomical features with flatworms, including a ventral nerve cord and an excretory system.

The ventral nerve cord runs along the underside of the worm and is responsible for transmitting nerve impulses throughout its body, while the excretory system helps to remove waste materials from the worm's body. These features are essential for the survival of Ascaris and are characteristic of many other parasitic and non-parasitic worms as well. Overall, the presence of these structures reflects the complex adaptations that worms have evolved in order to thrive in a variety of different environments.

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The statement that is false relative to the secondary structure of DNA is E) There is no restriction on the sequence of bases along a polynucleotide chain. The exact sequence carries the genetic information.The secondary structure of DNA refers to the double helix structure formed by the two polynucleotide chains.

The two chains are held together by hydrogen bonds between purine and pyrimidine bases. The purine and pyrimidine bases lie inside the helix, in planes perpendicular to the helical axis; the deoxyribose and phosphate groups form the outside of the helix.The sequence of bases along a polynucleotide chain is crucial in determining the genetic information carried by DNA. The sequence of bases codes for the production of specific proteins, which in turn determine an organism's characteristics. Therefore, there are specific rules for base pairing in DNA, such as the complementary base pairing of adenine with thymine and guanine with cytosine. These rules ensure that the sequence of bases in DNA accurately carries the genetic information.
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The three models of DNA replication that the Meselson-Stahl experiments were testing for were the conservative model, the semi-conservative model, and the dispersive model.

The conservative model proposed that the original double-stranded DNA molecule remained intact and produced a completely new double-stranded molecule. The semi-conservative model suggested that the original double-stranded DNA molecule separated and each strand was used as a template to synthesize a new complementary strand, resulting in two new double-stranded molecules, each with one original and one new strand. The dispersive model proposed that the original double-stranded DNA molecule broke apart and was dispersed randomly, with each resulting molecule containing pieces of the original DNA alternating with newly synthesized pieces.

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Overall, the addition of a top predator to the salt-marsh cordgrass ecosystem is likely to have significant impacts on the interactions among the species present, but the specific outcomes would depend on the predator added and the existing dynamics of the ecosystem.

If a top predator was added to the salt-marsh cordgrass (Spartina) ecosystem, it is likely that the trophic cascade would be disrupted, leading to changes in the interactions among the species present in the ecosystem. Depending on the specific predator added, there are several possible outcomes.
Option a, which suggests that the snail (Littoraria) would experience greater predation, could be a potential outcome if the new predator targeted Littoraria as a food source. This could lead to a reduction in the snail population and potentially affect the populations of other species that rely on Littoraria as a food source.
Option b suggests that Spartina would become the superior competitor among marsh plants. This is because the removal of a top predator could allow other herbivores to increase in abundance, which could then lead to overgrazing of other marsh plants. This could create an advantage for Spartina, as it is known for its ability to outcompete other marsh plants.
Option c suggests that the trophic cascade will remain the same with similar interactions among marsh species. However, the addition of a top predator is likely to have some impact on the interactions among the species in the ecosystem, even if the overall cascade remains intact.
Option d, which suggests that the fungus would have a greater colonization rate of Spartina, is unlikely to occur as a direct result of the addition of a top predator. However, changes in the population sizes of Spartina and other species in the ecosystem could indirectly affect the colonization rate of the fungus.
Option e, which suggests that the new predator would cause the salt marsh ecosystem to collapse, is also unlikely. While the addition of a top predator could have significant impacts on the ecosystem, it is unlikely to cause a complete collapse.
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Yes, palmitoleate synthesis can be carried out under strictly anaerobic conditions.

The biosynthesis of palmitoleate (cis-Δ9-hexadecenoic acid) starts with the precursor palmitate (hexadecanoic acid). The enzyme responsible for this conversion is called Δ9-desaturase, which introduces a cis double bond at the Δ9 position of the palmitate molecule. The reaction can be represented as follows:

Palmitate (hexadecanoic acid) + NADH + H+ + O2 → Palmitoleate (cis-Δ9-hexadecenoic acid) + NAD+ + H2O

Although this reaction typically requires oxygen (O2) as an electron acceptor, there are some microorganisms that possess alternative desaturase enzymes capable of using other electron acceptors, such as nitrate (NO3-) or fumarate (C4H2O4). These alternative desaturases allow the synthesis of palmitoleate to proceed under anaerobic conditions.

While the canonical biosynthesis pathway for palmitoleate requires oxygen, there are alternative pathways in certain microorganisms that can function under anaerobic conditions, allowing for the synthesis of palmitoleate in the absence of oxygen.

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Yes, a nebula that has collapsed to create new stars can appear red due to the presence of ionized hydrogen gas emitting light in the red part of the spectrum.

When a nebula collapses to form new stars, the intense radiation from the newly formed stars can ionize the surrounding hydrogen gas, causing it to emit light in specific wavelengths. This emission is known as hydrogen-alpha (H-alpha) emission, which falls in the red part of the spectrum. The presence of this red emission can give the nebula a reddish appearance. Additionally, other factors such as dust and the age of the stars within the nebula can also contribute to the color of the nebula. Therefore, a collapsed nebula that forms new stars can appear red or have a reddish hue.

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The levels of the organization listed, from simple to most complex, are white blood cells, human epithelium, heart, and central nervous system (CNS). These levels of organization demonstrate the increasing complexity of biological systems, with each level building upon the previous one to create more advanced structures and functions

White blood cells are the simplest of the group and are responsible for defending the body against infections and diseases. The human epithelium is the layer of cells that forms the outer surface of the body and helps to protect it from external threats. The heart is a more complex organ, composed of multiple types of tissues that work together to pump blood throughout the body. The CNS is the most complex system listed, consisting of the brain and spinal cord.

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Synthesizing glucose from pyruvate in gluconeogenesis requires more energy than is captured during glycolysis due to energy-consuming reactions and extra steps needed to bypass irreversible steps of glycolysis.

A. In catabolism, approximately 38% of the energy from oxidizing glucose to pyruvate is captured as NADH (+ H+) and ATP.

B. The rest of the energy from oxidizing glucose to pyruvate is released as heat.

C. Synthesizing glucose from pyruvate in gluconeogenesis requires around 62% more energy than captured during catabolism.

31. Main Answer: Humans need to breathe oxygen because it acts as the final electron acceptor in the process of cellular respiration, allowing for efficient energy production.

A. Cellular respiration, specifically oxidative phosphorylation, requires oxygen in human metabolism.

B. Oxygen reacts with electrons and protons captured by carriers in catabolism to form water.

C. Carrier molecules like NADH and FADH2 carry the captured material before it reacts with oxygen.

D. The energy released when captured material reacts with water is used to produce ATP.

E. In the absence of oxygen, the captured material undergoes anaerobic respiration or fermentation, leading to less efficient energy production.

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because of high wind, low moisture and cold temperature trees don't grow on top of the hill because above alleviation trees cannot grow