Based on your understanding of nucleic acids, what type of bonds form between the CRISPR/guide RNA molecule and the target DNA

Answers

Answer 1

Answer:

The single guide RNA forms hydrogen bonds with DNA, while Cas9 hydrolyzes phosphodiester bonds

Explanation:

The base pairing between nucleic acid strands (either DNA or RNA) is through hydrogen bonds between nucleotide bases. In DNA, Adenine always forms two hydrogen bonds with Thymine, while Guanine always forms three hydrogen bonds only with Cytosine. Moreover, adjacent nucleotides in the same strand are covalently linked by phosphodiester bonds (i.e., covalent bonds between the 5' phosphate group of one nucleotide and the 3'-OH group of another). The CRISPR/Cas9 genome editing systems make use of single-guide RNAs (sgRNAs) that interact with DNA through hydrogen bonds. These sgRNAs have perfect complementarity to the target DNAs in order to bind them. On the other hand, Cas9 is an enzyme that hydrolyzes phosphodiester bonds in both DNA strands very precisely and accurately by using a sgRNA complementary to a specific DNA sequence.


Related Questions

As with any major scientific discovery, the previous work of many different scientists help contribute to final conclusions. Which of the following scientists' work did not contribute to the discovery of the structure of the DNA molecule? A Gregor Mendel B Linus Pauling C Erwin Chargaff D Rosalind Franklin​

Answers

A

AExplanation:

AExplanation:Gregor Mendel is generally regarded as the father of genetics due to his three(3) laws of genetics. Non of his work alluded to the existence of the DNA molecules( i.e. the major constituent of genes) let alone its structure.

convention cells hbnmb,

Answers

Answer:

convention cells

Explanation:

In the field of fluid dynamics, a convection cell is the phenomenon that occurs when density differences exist within a body of liquid or gas. These density differences result in rising and/or falling currents, which are the key characteristics of a convection cell. When a volume of fluid is heated, it expands and becomes less dense and thus more buoyant than the surrounding fluid. The colder, denser part of the fluid descends to settle below the warmer, less-dense fluid, and this causes the warmer fluid to rise. Such movement is called convection, and the moving body of liquid is referred to as a convection cell. This particular type of convection, where a horizontal layer of fluid is heated from below, is known as Rayleigh–Bénard convection. Convection usually requires a gravitational field, but in microgravity experiments, thermal convection has been observed without gravitational effects.

An enzyme that breaks DNA, dispels the tension, and reseals the strand ahead of a DNA replication growing fork is called a(n):

Answers

Answer:

Topoisomerase

Explanation:

Topoisomerases are enzymes that produce changes in the topology of the DNA during replication, transcription, traduction, or reparation processes. They can cut one or both strands and in order to relieve torsional stresses in the supercoiled structure of DNA. With this, they help to maintain the chromosome's integrity. There are two types of topoisomerases: topoisomerase I (it cuts only one strand of DNA) and topoisomerase II (it is able to cut both strands of DNA).

Advantages and disadvantages of physical barriers in the immune system

Answers

Explanation:

advantages:Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

What is immune system?

Immune system is a large network of organs, white blood cells, proteins (antibodies) and chemicals. This system works together to protect you from foreign invaders (bacteria, viruses, parasites, and fungi) that cause infection, illness and disease.

Disadvantages of physical barriers in the immune system is causing immuno deficiency disease.

For more information regarding immune system, visit:

https://brainly.com/question/234802

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what kind of graph is this?​

Answers

Answer:

Explanation:

financial chart

Financial chart:):):):););););)

A fruit fly of genotype B R/b r is testcrossed with b r/b r. In 84 percent of the meioses, there are no chiasmata between the linked genes; in 16 percent of the meioses, there is one chiasma between the genes. What proportion of the progeny will be B r/b r

Answers

Answer:

4 percent (4%)

Explanation:

A single crossover occurs between two (non-sister) chromatids belonging to homologous chromosomes. In this case, 16 percent of the meioses have a single crossover, thereby it will produce 8 percent of the chromosomes with the original (parental) combination in the progeny and the remaining 8 percent should be recombinants. From this result, it is reasonable to conclude that half of these recombinants should be 'Br' (and the other remaining 4 percent should be recombinants 'bR'), and therefore the answer is 4 percent (4%).

One way to think about the importance of biodiversity is simply to think in terms of natural capital: the roles that genes, species, ecosystems, and ecological processes play in supporting human society and a healthy biosphere. What are benefits provided by biodiversity?

Answers

Answer and Explanation:

Biodiversity composes of different species living in a certain restricted area. It is the variability of living beings in a natural environment, involving all the species on the planet and their genetic material. This variety includes all plants, animals, fungus, and microorganisms living in a certain place, their genetic variability, and the ecosystems these species form.

Ecosystems include all the biotic, physical, and chemical components of nature, continuously interacting and depending on each other. The ecosystem is composed of living and non-living things that interact and depend on each other. These are the biotic and abiotic factors.

Biodiversity, in equilibrium with abiotic factors, provides ecosystem services, which are important for the maintenance of all forms of life, including human beings.  

Ecosystem services

When talking about ecosystem services, we are referring to the benefits that ecosystems provide us. There are four types of ecosystem services:

Provision or supply:

These are the products obtained by nature to be used or consumed, directly or with previous processing. Among them, we might mention food, clear freshwater, fertile soils, geotic materials, raw materials (that can be used for clothing, construction), renewable fuels, biochemical compounds, pharmacological/medicinal resources, among others.

Regulation:

Refers to ecological processes that improve life. Among others, we might mention climate regulation, CO² absorption, water purification, air purification, erosion control, natural disaster avoidance such as flooding, soil fertility maintenance, waste recycling, pests/plagues control, etc.

Cultural:

Refers to not the material benefits. Ecosystems provide a recreational place or area to spend time and learn. They provide a spiritual/religious space, are a source of inspiration. Ecosystems are cultural patrimony and the source of scientific knowledge.

5 factors that influence price​

Answers

Explanation:

Price Determination: 6 Factors Affecting Price Determination of Product

Product Cost: The most important factor affecting the price of a product is its cost. ...

The Utility and Demand: ...

Extent of Competition in the Market: ...

Government and Legal Regulations: ...

Pricing Objectives: ...

Marketing Methods Used:


What is the original source of energy for most ecosystems?
A grass
B) humans
C)the sun
D)heat

Answers

Answer:

C the sun

Explanation:

Step by step explanation

the answer would be C. because the suns vitamins and energy is all around for 12 hours a day

In screenshot! answer quickly please

Answers

Answer:

umm i would answer but i cant see the photo whats the question?

Explanation:

21. Which force is used to separate solid pollutant particles from smoke given out from factories?
onlar​

Answers

Answer: Electrostatic Force

Explanation:

Electrostatic force is used to separate solid pollutant particles from smoke given out from factories


What is an element/compound that is
cycled through ecosystem?

Answers

Answer:

Nitrogen, carbon, and phosphorus are just a few

Explanation:

Help please

What factors can influence the carrying capacity of an ecosystem?(CHOOSE ALL THAT APPLY)
A The number of predators
B The amount of available food
C The amount of water
D The amount of sunlight
E The water clarity of an aquatic ecosystem

Answers

Answer:

ABCD

Explanation:

All of the above except e I think

BRAINLIEST
CAN SOMEONE PLZ HELP ME!!

Answers

Explanation:

I’m pretty sure it’s phenotypes

The North Slope of Alaska has concentrations of air pollutants comparable to the air pollutant concentration of some major cities on the east coast of the United States because

Answers

Answer:

pollutants are transported by are from Eurasia

Explanation:

What is the answer to number 1

Answers

Answer:

TTT GGC AGT

Explanation:

Question 12 of 15
A force is a push or pull.

Answers

Answer:

True

Explanation:

A force is a push or pull against something or someone

According to my research it’s true

Why consist of a cell body that contains the 

Answers

Answer:

The cell body, also called the soma, is the spherical part of the neuron that contains the nucleus. The cell body connects to the dendrites, which bring information to the neuron, and the axon, which sends information to other neuron

Explanation:

Mark brainliest pls

The enzyme dihydrofolate reductase (DHFR) normally resides in the cytosol, and it can be imported into mitochondria by appending a mitochondrial signal sequence. However, when this modified DHFR is incubated with methotrexate, which is a substrate analog that binds tightly to the active site, the modified DHFR is no longer imported. Propose an explanation for this finding.

Required:
a. Consider a transmembrane protein that spans the inner nuclear membrane. Suggest a pathway by which such a protein could reach its destination.
b. Clathrin can assemble with multiple types of adaptors to create coated vesicles. Explain why this multiplicity of adaptors is useful to the cell

Answers

Answer:

Within the mitochondria, the import takes place post-translationally. The imported protein must be in an unfolded condition so that it can go through the mitochondria's import machinery. In the existence of methotrexate, the bound DHFR moiety remains in the folded state, and thus, stays on the surface of mitochondria.

It is witnessed that the presequence must be of the length of 55 amino acids to cover both the membranes of mitochondria and must be of about sixty amino acids length to associate with the mhsp70. If the presequence of this methotrexate bound DHFR is of seventy-five amino acids in length, it can associate with mhsp70 within the matrix, to initiate the ATP reliant import within the mitochondria. Due to the presence of various endocytic and intracellular sorting signals, multiple adaptors are essential.

A very common dahlia plant with red petals is crossed with another very common plant having streaky petals. The probability that an offspring from this cross has red flowers is .75. Let X be the number of plants with red flowers grown from 100 randomly collected seeds from a very large population (N > 10,000) of this cross. A. Does the random variable X have a binomial distribution

Answers

Answer:

Yes, it is vivid that the random variable has a binomial distribution

Explanation:

From the given information:

The probability of the plant with red flowers(p success) = 0.75

the sample size n = 100

Thus, the mean μ = np

= 100 ×0.75

= 75

The standard deviation can be calculated by using the formula:

[tex]SD = \sqrt{np(1-p)}[/tex]

[tex]SD = \sqrt{100 \times 0.75(1-0.75)}[/tex]

[tex]SD = \sqrt{100 \times 0.75(0.25)}[/tex]

[tex]SD = \sqrt{18.75}[/tex]

SD = 4.33

Plz someone help. This is due tonight!!!! I will give brainiest if you fill it out and NO LINK!!

Answers

Answer: I am not sure i will try to figure out

Explanation:


In the following choices, the one that is NOT a function of the skeletal
system is
O protect organs
make red blood cells
transport blood cells
O provide shape and support

Answers

Answer:

I'm pretty sure it's transport blood cells

BRAINLIEST
PLZZ HELP ME !!!

Answers

Answer:

the answer is in picture

Which of the following is NOT an
ecological effect of mining?
A. habitat disruption
B. loss of biodiversity
C. acid mine drainage
D. increased biodiversity

Answers

d is correct

..................

^_^

The vertical acis on a graph is called the y-axus for scientific data what usually placed along the y-axis

Answers

Answer:

dependent variables

Which of the following is the most susceptible to damage by pollution in the air and
precipitation

A. The roots of trees
B. Trees at high elevations
C. Birds that migrate seasonally
D. Plants that grow in lakes or rivers

Answers

Answer:

B) Trees at high elevations

Explanation:

The atmospheric conditions and precipitation toxicity is most likely to affect trees at high elevations more so than the other answers.

The tree leaves, which are the main key to the tree's photosynthesis, absorb the oxygen and Co2 from the air, which are converted into nutrients. Without proper nutrients, the trees may die out.

PLEASE HELP! I WILL GIVE BRAINLIEST! the factors that limit a populations growth are A.oxidation and corrostion B.climate and weather C.command and control

Answers

Hello! Let's tackle this question together.

The answer is option B.

Climate and weather have a significant monopoly over whether or not a species will survive. Say if we put a plant that has to live in hot areas in a cold place. It will die because of the climate and weather in the area. Henceworth, the answer is B.

g In transcription, the 3'-hydroxyl group of the growing RNA strand: Group of answer choices NONE OF THESE binds to the 5' ribose. attacks the outermost phosphorous group of the incoming nucleotide. ALL OF THESE attacks the 5' hydrogen of the incoming nucleotide. interacts with a positively charged metal ion in the active site.

Answers

Answer:

attacks the outermost phosphorous group of the incoming nucleotide.  

Explanation:

Transcription is the biological process where RNA is formed. As you may already know, RNA is an extremely important nucleic acid for genetic processes and the production of proteins. The transcription has three steps that are called start, elongation and end. In the elongation phase, the RNA strand is growing, at this time, RNA polymerase, which is the enzyme responsible for RNA elaboration, places the nucleotides, in the RNA strand, in the 5' - 3' direction. This enzyme causes the hydroxyl, which makes up the nucleotide at the 3' end of the forming RNA, to attack the phosphorus present in the incoming  ribonucleotide.

Beatrice’s science teacher gave her an unknown substance to identify based on its properties. After a few tests, Beatrice listed her observations in a chart.

reflects light very well
warms up in my hand
flattens out when hit with a hammer
conducts electricity

Which kind of substance does Melinda have?
A. metalloid
B. nonmetal
C. metal
D. noble gas

Answers

b. nonmetal i just took it

5. What do Chloroplast and Mitochondria have in common?
A. They contain DNA
B. Provide energy to the cell
C. Are in charge in cell transport
D. Make proteins

Answers

Answer:

B. Provide energy to the cell

Explanation:

Both the chloroplast and the mitochondrion are organelles found in the cells of plants, but only mitochondria are found in animal cells.

The function of chloroplasts and mitochondria is to generate energy for the cells in which they live.

The structure of both organelle types includes an inner and an outer membrane

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