A student makes a new gel using agarose, water, and buffer solution. The student loads their DNA samples in their wells and places the gel in the chamber for an appropriate length of time. When the student places the gel on the UV lightbox, no DNA bands show up at all. Even the marker lane is clear and has no bands! What mistake did the student make? Explain your answer.

Answers

Answer 1

Answer:

A dye needs to be added to the gel electrophoresis

Explanation:

Ethidium bromide (EtBr) is a reagent that adheres to DNA and fluoresces under ultraviolet (UV) light. Due to this feature, EtBr is widely used to dye DNA strands in molecular biology techniques such as agarose gel electrophoresis. The most common dyes for visualizing and staining nucleic acids during gel electrophoresis include EtBr, SYBR gold, Eva green and SYBR safe. If EtBr (or any other staining agent) was not added to the electrophoresis gel and/or running buffer, the gel will be dark under UV light (even the ladder lane will look empty) because DNA has not been stained.

Answer 2

The mistake the student made was by not adding dye to the sample. Dyes

are commonly used during electrophoresis.

Agarose gel electrophoresis is the process which helps in the separation

and purification of DNA samples. This process involves the use of dyes

which acts as markers.

in this scenario, we were told no DNA bands show up and the marker lane is

clear means no dye was added.  The common dye used in agarose gel

electrophoresis is Ethidium bromide (EtBr) which should be added by the

student.

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Related Questions

Can you guys help me please I’ve gotten to many wrong already.

Answers

Answer:

I would say A

Explanation:

I think it’s also AAAAAAAAAAAAAAAA

The graph above gives the land surface air temperatures and the sea surface water temperatures from 1950 to 2016. Global
warming encompasses the changes in climate and temperature around the globe from the oceans to on land. However, when
studying the warming of Earthy's land and water temperatures, a difference between the two can be observed. From the graph, it
can be seen along most of the graph that the water temperature of the sea's surface remained lower than that of the land's surface
air temperature. What unique characteristic of water allows for this phenomenon to occur?

a. water’s heat of fusion
b. water’s conductivity of heat
c. water’s heat of vaporization
d. water’s specific heat capacity

Answers

Answer:

D

Explanation:

Waters specific heat capacity

Which type of organism would MOST likely benefit directly from increased levels of CO2?

- fish

- bird

-tree

- mushroom

Answers

Answer:Im pretty sure its a tree or mushroom

Explanation:

The study of microorganisms is called microbiology.

The correct answer to the question is option D which is mushroom.

What is anaerobic respiration?Anaerobic respiration is respiration using electron acceptors other than molecular oxygen.Although oxygen is not the final electron acceptor, the process still uses a respiratory electron transport chain.

According to the question, the fungi are saprotrophic species and an increased level of CO2 will help them to grow.

Hence, the correct answer is option D.

For more information about anaerobic respiration, refer to the link:-

https://brainly.com/question/14688752

Does the distance from earth to sun determine the seasons? please explain

Answers

No it’s the way the earth is rotating

Answer:

nope

Explanation:

Contrary to popular belief, the seasons are not determined by the earth's distance from the sun. The seasons are actually caused by the earth's tilted axis. Because the axis is tilted, when the Northern Hemisphere is tilted towards the sun, it's warm.

hope this helps:)

name 4 Flowers that are polinated by animals

Answers

Answer:

Butterflies , Bees , Bats , Humming Bird , Lemurs and Honey Possum are some examples of flowers which are pollinated by Animals.

Hope it helps you!!!

What is the role of the nervous system in the body?

A. To gather and respond to information
B. To protect other organs and tissues
C. To send messages using blood cells
D. To transport oxygen and carbon dioxide

Answers

Answer:

A. To gather and respond to information

Explanation:

please help me with this homework question!

Answers

Answer:

Tt and tt

Explanation:

To solve this just work backwards. The first box has Tt so one parent has to have T and the other t. Since the tall plant is on the top, it is T_ and t_. Next use the second box (tt). They are both t so the final answer is Tt x tt.

Anaerobic bacteria releases methane, hydrogen oxides, and oxygen.
True or false ?

Answers

the answer is true, it does



Which pair of statements correctly describes a way that the chromatin contributes to gene expression?

Answers

Answer:

Chromatin is responsible for gene expression.

Explanation:

Chromatin is responsible for gene expression because chromatin contains DNA that has all information about genes. chromatin controls the gene in order to form encoded products. DNA in chromatin send information to the RNA to produce specific types of proteins that are needed by the organisms for its growth and development so in that way the genes can be expressed in the organism.

There are two types of joints. One is movable and the other is called what?
flexible
muscular
immovable
optional

Answers

Answer:

Knee

Explanation:

I thinko

Immovable if I remember correctly

Question 24 of 35
Which two statements describe the Mesozoic era?
A. Most of Earth's history took place within this time.
B. Plants began to colonize land during this era.
✓ C. It is also known as the Age of Reptiles.
D. It lasted from about 250 million years ago to 70 million years ago.

Answers

I’ve done this awhile back I’m pretty sure it’s C and D

Answer:

C

Explanation:

it was the time to f the dinosaurs and some of the dinosaurs were reptiles

PLEASE HELP(this question has been asked a lot of times by other people with no good answers.)

1. Which of the following statements about classification of organisms is true?

A: Phylogenetic classification uses genetics and ancestral relationships to classify organisms.

B: The Linnaean system of classification takes into account ancestral relationships between organisms

C: Linnaeus concentrated on the genetic characteristics of organisms to classify them.

D:In phylogenetic classification, the group that organisms are put into is called a taxon.

Answers

Answer:

Phylogenetic classification uses genetics and ancestral relationships to classify organisms.

Explanation:

took the test

The correct option is (a) Phylogenetic classification uses genetics and ancestral relationships to classify organisms.

Phylogeny classifies species based on evolutionary kinship or evolutionary distance (taxonomy). The degree and type of evolutionary distance between organisms determines their phylogenetic connection. Let's define taxonomy in order to better comprehend this idea.

The connection between classification and phylogeny?The study and comparison of traits as well as the comparison of groups of organisms are the foundations of both phylogeny and categorization. However, there is a distinct feedback between character interpretation and inferred phylogeny, which is seen in the weighting of the characters used to construct the classification.

Learn more about classification and phylogeny brainly.com/question/16452940

#SPJ2

2. Students survey the area in and around a small
pond and find the following: algae, rocks,
tadpoles, snails, mud, water. Which items
represent biotic factors? Select all that apply.
algae
® rocks
© tadpoles
O snails
E mud
f water

Answers

Answer:

Algae, tadpoles, snails

Explanation:

Based on the context clue in the
sentence below, what does
brilliant mean?
The ball was a brilliant blue in
stark contrast to the dull, tan
walls of the racquetball courts.
A. dazzling
B. round
C. stuffy

Answers

Answer:

i belive is: A. Dazzling

Answer:

dazzling

Explanation:

when they use brilliant in this context, they are seemingly emphasizing the beauty in the pigment of the ball.

How much longer is an average day in May compared to October?
i give branily

Answers

Answer:

There is no difference there are 31 days in May and October.

Explanation:

True or false

Organisms that are in the sane kingdom are always in the same order


True or false

Organism of the same species are also in the same class

True or false

Organism that are in the same phylum have more in common than organism the same order

Answers

Answer:

Falae - True - False

Explanation:

Kingdom - Phylum - Order - Class - Genus - Family - Species

stars with greater overall mass are ___ and ___, and they consume their nuclear fuel

1. brighter/dimmer
2. cooler/hotter
3. faster/slower​

Answers

Answer:

Stars with a greater overall mass are  

1. (brighter)

and  

2. (hotter)

, and they consume their nuclear fuel  

3. (faster)

.

Explanation:

Answer:

Brighter, hotter, faster

Explanation:

Answer for edmentum test

My test is timed, who ever answer this first will be Brainliest, How many genes for each trait does a child inherit?

Answers

Answer:

One allele for every gene in an organism is inherited from each of that organism's parents.

Explanation:

Answer:

These two copies of the gene contained in your chromosomes influence the way your cells work. The two alleles in a gene pair are inherited, one from each parent.

Explanation:

Peptidoglycan is the material found in the bacterial____
that forms a molecular basis of the Gram stain.
_organelles
_cell wall
_nucleus

Answers

Answer:

The Cell Wall of Bacteria

Bacteria are protected by a rigid cell wall composed of peptidoglycans.

LEARNING OBJECTIVES

Recall the characteristics of a bacterial cell wall

KEY TAKEAWAYS

Key Points

A cell wall is a layer located outside the cell membrane found in plants, fungi, bacteria, algae, and archaea.

A peptidoglycan cell wall composed of disaccharides and amino acids gives bacteria structural support.

The bacterial cell wall is often a target for antibiotic treatment.

Key Terms

binary fission: The process whereby a cell divides asexually to produce two daughter cells.

image

Bacterial Cell Wall: The anatomy of bacterial cell structure.

Bacterial cells lack a membrane bound nucleus. Their genetic material is naked within the cytoplasm. Ribosomes are their only type of organelle. The term “nucleoid” refers to the region of the cytoplasm where chromosomal DNA is located, usually a singular, circular chromosome. Bacteria are usually single-celled, except when they exist in colonies. These ancestral cells reproduce by means of binary fission, duplicating their genetic material and then essentially splitting to form two daughter cells identical to the parent. A wall located outside the cell membrane provides the cell support, and protection against mechanical stress or damage from osmotic rupture and lysis. The major component of the bacterial cell wall is peptidoglycan or murein. This rigid structure of peptidoglycan, specific only to prokaryotes, gives the cell shape and surrounds the cytoplasmic membrane. Peptidoglycan is a huge polymer of disaccharides (glycan) cross-linked by short chains of identical amino acids (peptides) monomers. The backbone of the peptidoglycan molecule is composed of two derivatives of glucose: N-acetylglucosamine (NAG) and N-acetlymuramic acid (NAM) with a pentapeptide coming off NAM and varying slightly among bacteria. The NAG and NAM strands are synthesized in the cytosol of the bacteria. They are connected by inter-peptide bridges. They are transported across the cytoplasmic membrane by a carrier molecule called bactoprenol. From the peptidoglycan inwards all bacterial cells are very similar. Going further out, the bacterial world divides into two major classes: Gram positive (Gram +) and Gram negative (Gram -). The cell wall provides important ligands for adherence and receptor sites for viruses or antibiotics.

THANKS ME LATER IF YOU IF YOU LIKE IT

That is a big answer. ^ but yeah its cell wall.

When two species occupy exactly the same niche, one species will be eliminated from the community by _____.
A.
a secondary disturbance
B.
the superior competitor
C.
the keystone species
D.
a primary disturbance

Answers

Answer:

Answer is B

Explanation:

Hope it helps

Animals are an example of me. Prokaryotic cell
Prokaryotic cell
Eukaryotic cell
Both types of cells.

Answers

this answer is eukaryotic cell because eu means nucleus & pro means no nucleus

The Industrial Revolution ushered in all of the following EXCEPT:
A. Higher infant mortality rates
B. Exponential growth in the human population
C. Antibiotics and vaccinations
D. Longer life expectancies

Answers

Answer:

Option A. is correct

Explanation:

In Europe and the United States, the Industrial Revolution was the transition to new manufacturing processes that took place from the 18th to 19th centuries.

The Industrial Revolution ushered in all of the following EXCEPT Higher Infant Mortality Rates.

Causes of Industrial Revolution:

1. Emergence of capitalism

2. European imperialism

3. Capitalism

So,

Option A. is correct

please help 20 points if so.

Answers

Answer: A. Codominance is a blending of the two traits, and incomplete dominance expresses both traits equally.

Explanation: Good luck! :D

Answer:

A is correct

Explanation:

who were the frist people who came to guyana​

Answers

Hello,

The first human inhabitants of Guyana probably entered the highlands during the 1st millennium bce. Among the earliest settlers were groups of ArawakCarib, and possibly Warao (Warrau).The Dutch were the first Europeans to settle modern-day Guyana. The Netherlands had obtained independence from Spain in the late 16th century and by the early 17th century had emerged as a major commercial power, trading with the fledgling English and French colonies in the Lesser Antilles.

(The Words That Are BOLD are the answers.)

Have a great day/night and stay safe! :)

Answer:

Amerindians

The first people who came to Guyana to settle was the Amerindians.

Cell Junctions help in moving up a level in the hierarchy of organization of living systems. When cell junctions from, the system goes from the cell level to the _________ level.

Answers

Answer:
Cell Junctions help in moving up a level in the hierarchy of organization of living systems. When cell junctions from, the system goes from the cell level to the _tissue_ level.

i need help plz !!!!

Answers

Answer:

Black

Orange

Black and white, orange and white.

Explanation:

All of these options are possible for colors in males except black, orange, and white. This phenotype requires 2X chromosomes, one with the black allele and one with the orange allele.

Can someone please give me some main ideas for a scientific story? It has to be at least 500 words and I'm not sure what to write about. Please help

Answers

Explanation:

try ancient sciences from Greece and how it compares to modern science

What are the effects of enzymes on chemical reactions? Select all of the answers that apply.
A. Enzymes increase the heat of reaction.
B. Enzymes decrease the activation energy.
C. Enzymes increase the rate of the reaction.
D. Enzymes change the ideal pH for a reaction to proceed.
E. Enzymes provide a site where reactants can be brought together.

Answers

Stage One: Autolysis

The first stage of human decomposition is called autolysis, or self-digestion, and begins immediately after death. As soon as blood circulation and respiration stop, the body has no way of getting oxygen or removing wastes. Excess carbon dioxide causes an acidic environment, causing membranes in cells to rupture. The membranes release enzymes that begin eating the cells from the inside out.

Rigor mortis causes muscle stiffening. Small blisters filled with nutrient-rich fluid begin appearing on internal organs and the skin’s surface. The body will appear to have a sheen due to ruptured blisters, and the skin’s top layer will begin to loosen.

Stage Two: Bloat

Leaked enzymes from the first stage begin producing many gases. The sulfur-containing compounds that the bacteria release also cause skin discoloration. Due to the gases, the human body can double in size. In addition, insect activity can be present.

The microorganisms and bacteria produce extremely unpleasant odors called putrefaction. These odors often alert others that a person has died, and can linger long after a body has been removed.

Stage Three: Active Decay

Fluids released through orifices indicate the beginning of active decay. Organs, muscles, and skin become liquefied. When all of the body’s soft tissue decomposes, hair, bones, cartilage, and other byproducts of decay remain. The cadaver loses the most mass during this stage.

Stage Four: Skeletonization

Because the skeleton has a decomposition rate based on the loss of organic (collagen) and inorganic components, there is no set timeframe when skeletonization occurs.

all you need is in the photo

ASAP​

Answers

Answer:

D

Explanation:

What type of mutation is shown in the DNA strand to the right given the original strand.



original - T A T G A C A A T ---> mutated strand - T A T C A C A A T

Group of answer choices

point mutation

deletion

insertion

translocation

Answers

the answer is insertion
Correct answer - Point mutation.

Why? - A point mutation or substitution is a genetic mutation where a SINGLE nucleotide base is changed, inserted or deleted from a DNA or RNA sequence of an organism's genome.

Why not Insertion - In genetics, an insertion (also called an insertion mutation) is the addition of one or more nucleotide base pairs into a DNA sequence. This can often happen in microsatellite regions due to the DNA polymerase slipping. ... On a chromosome level, an insertion refers to the insertion of a larger sequence into a chromosome. So, in short Insertion adds one nucleotide but not changes one nucleotide to another. Which is done by POINT MUTATION.
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