Biology College

A group of researchers transformed E. coli to express
dsRNA that matched a transcription factor, eaf-1. They
then fed these E. coli to C. elegans worms. When the
researchers examined the C. elegans, they found that
they had fewer offspring and were smaller individuals,
with similar characteristics to C. elegans in which eaf-1
had been knocked out.
The students suggested several hypotheses based on
these observations about how RNAi worked:
1. The dsRNA inhibited gene transcription.
2. The dsRNA inhibited mRNA processing.
3. The dsRNA inhibited translation of mRNA into protein.
4. The dsRNA inhibited protein folding.
Mark this and return
The researchers then performed a series of experiments
to determine which hypothesis was correct. The C.
elegans were found to be transcribing eaf-1 into mRNA,
but not producing eaf-1 protein. When the students
directly injected C. elegans with dsRNA and tracked
tagged mRNA, they found the mature mRNA was
degraded in the cytoplasm, and ribosomes were not
binding to it. Which hypothesis is supported by these
observations?

Answers

Answer 1

The hypothesis that is supported by these observations is that the dsRNA inhibited the translation of mRNA into protein (third option).

What does the experiment reveal about dsRNA?

In this experiment, the C. elegans worms were transcribing eaf-1 into mRNA but not producing the eaf-1 protein. This phenomenon shows that dsRNA inhibited the translation of mRNA into protein related to the action of E. coli on the worms.

This also explains why the worms affected by this bacteria had fewer offspring and the offspring were smaller.

Learn more about RNA in https://brainly.com/question/4120168

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