You plate colonies of E. coli with the lac operon genotype of I-P+O+Z+CAP+ on 1) a medium containing only glucose, and 2) a medium containing only lactose. You isolate mRNA from bacteria from each medium and do a Northern blot with a probe for β-galactosidase. Draw the results.

Answers

Answer 1

A complete collection of single-use and high-throughput electrocompetent and chemically competent E. coli

Choosing the ideal competent cells for your cloning applications and workflows is a critical component of success. Aspects to consider when selecting your competent E. coli strain include:

Transfection method – chemical versus electroporation

Transformation efficiency – bacterial strain and genotype

Downstream application – protein expression, library production, general cloning


Related Questions

what is sex?(explain it )

Answers

Sex is a reproduction system involving both male and female to partake in sexual activity intercourse to create an offspring between the two beings creating a living organism

What protein involved in coagulation provides the activation for the final step in clotting?
A) fibrin
B) thrombin
C) prothrombin activator
D) fibrinogen

Answers

Answer:

b thrombin

Explanation:

thrombin catalyzes the activation of the molecules that are present in plasma

The protein thrombin involved in coagulation provides the activation for the final step in clotting. Thus, the correct option is B.

What is Clotting?

Clotting may be defined as the procedure in which blood alters into a solid state to construct a thick mass or lump onto the surface of the injured region.

The protein thrombin catalyzes the final step in clotting which transforms fibrinogen into fibrin. Then such fibrin monomers polymerize and construct a mess of fibrin polymers which results in a fibrin clot.

Therefore, the correct option for this question is B.

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Most of a cell's molecules can be classified as belonging to one of four major classes: lipids, carbohydrates, nucleotides, or amino acids. Classify the five molecules into one of the four major classes.

Answers

Answer:

Diagram, in attachments

Explanation:

From the left side of the screen to the right,that is from a structure with a sugar structure with two other molecules  attached to it.

The first molecular structure is Nucleotides. Reasons it contains the middle ribose sugar(5-carbon)connected to the phosphate group and  Nitrogenous bases.This is the structure of nucleotides  and when this is joined by phosphodiester bond between one a nucleotide, to the phosphate group of another  nucleotide molecule  it forms a nucleic acid molecule.

The second structure from left to right with  long carbon chains, it is  a lipid.That js an Ester formed from the reaction of fatty acids with alcohol glycerol.

However, the presence of Phophate group in structure makes it different from a normal tryglycerides.The phosphate group has replaced  one of the  the three fatty acid molecules.Therefore it is called Phospholipid.With one hydropholic ends(phosphate ends) and the hyrophobic end the  carbon chain,with one unsaturated. The lipids molecules are held together my ester bonds.

The next structure is the protein structure(dipeptide).Genrally amino acid is made up of the central Alpha carbon connected on the right by the Carbonyl group(coo-) on the left by the Amine(NH2) group.The R-group which determines the type of amino acids  and hyrdogen atom.In the above structure condensation reaction  has occurred between the hydrogen atom  of  the amine group and carbonyl group of the other amino unit to form a dipeptide.The  bond formed from the condensation is the peptide bond.

The last structure on the far right are the carbohydrate -ring structure and the straight chains.The functional groups of the CHO  -Carbonyl group and hydroxyl group are glues to this.

Which of the following will increase the gravitational force exerted by one object on another? Increasing the temperature of the objects Decreasing the temperature of the objects Decreasing the distance between the objects Increasing the distance between the objects

Answers

The correct answer is C. Decreasing the distance between the objects

Explanation:

Gravitational force and distance between the objects involved are inversely proportional variables; this means gravitation increases when the distance decreases. An example of this is that the Sun's gravitational force strongly attracts objects near it including planets in our Solar system, which causes planets to orbit the Sun. However, the force the Sun exerts on planets in other solar systems or in other galaxies is low due to the distance between this and the sun. In this context, to increase the gravitational force the distance between the objects should be decreased.

Answer: C

Explanation:

How many years of cumulative evolution separate chimpanzees from humans? Group of answer choices 7-8 million years 2-3 million years 130-140 million years 130-140 thousand years 14-16 million years

Answers

Answer:

7-8 million years

Explanation:

There is many scientific evidences present from which scientists concluded that the humans evolved from chimpanzees about 7-8 million years and the ability to walk on two legs evolved about 4 millions year ago. There are a variety of physical and behavior changes occurred in the human while evolving from apes or chimpanzees. It takes millions of years to change occurs in the DNA of an organism.

. All living things are made of cells

Answers

Answer:

yes all living things[animals and plants ] have their own cells

Explanation:

hav a nice day :}

Glycoconjugates result from the covalent linkage of carbohydrate to proteins or:________.A. Amino acids B. Lipids C. Nucleotides D. Both A and B are correct E. None of the above are correct

Answers

Answer:

B. Lipids

Explanation:

Glycoconjugates occurs as a result of a process known as glycosylation.

Glycoconjugates result from the covalent linkage of carbohydrate to proteins or lipids.

It is also used to refer to carbohydrates who are covalently bonded to other chemical compounds such as protein, lipids, peptides etc.

In what ways is eukaryotic replication similar to bacterial replication, and in what ways is it different

Answers

Answer:

Ways in which DNA replication is similar in eukaryotes and bacteria:

Replication is semiconservative.

Replication origins serve as starting points for replication.

A short segment of RNA called a primer provides a 3′-OH group for DNA polymerases to begin the synthesis of the new strands.

Synthesis is in the 5′-to-3′ direction.

The template strand is read in the 3′-to-5′ direction.

Deoxyribonucleoside triphosphates are the substrates.

Replication is continuous on the leading strand and discontinuous on the lagging strand.

Ways in which eukaryotic DNA replication differs from bacterial replication:

There are multiple origins of replications per chromosome.

Several different DNA polymerases have different functions.

Immediately after DNA replication, nucleosomes are assembled.

Explanation:

Sorry that is long but i hope it helps!

Which is the experimental group?

Answers

In this example, your experimental group is the bag of popcorn you placed in the refrigerator. An experimental group is the group that receives the variable being tested in an experiment. The control group is the group in an experiment that does not receive the variable you are testing.

Answer:

An experimental group is a group that receives a treatment in an experiment.The "group" is made up of test subjects.(people, animals,plants, cells,etc.) and the "treatment" is the variable you are studying.

An experimental group is also known as a treatment group.

Classify each statement based on whether it describes prokaryotes only, eukaryotes only, or can describe both prokaryotes and eukaryotes.
1. unicellular
2. contain mitochondria
3. are generally less than 2 pm
4. multicellular
5. lack membrane-bound organelles

Answers

Answer:

unicellular - both prokaryotes and eukaryotes

contain mitochondrion - eukaryotes only

are generally less than 2 pm - Prokaryotes only

multicellular - eukaryotes only

lack membrane-bound organelles - prokaryotes only

Explanation:

Prokaryotes are generally unicellular, that is, they are made up of single cells only. However, there are unicellular and multicellular eukaryotes with some eukaryotes like humans and advanced plants having as many as millions of cells.

Prokaryotes generally lack nucleus and other membrane-bound organelles such as chloroplast and mitochondrion. Eukaryotes on the other hand have nucleus and membrane-bound organelles such as mitochondrion and chloroplast.

When it comes to size, prokaryotes are generally small and microscopic while eukaryotes consist of both microscopic and macroscopic cells or organisms. However, prokaryotes are generally smaller than microscopic eukaryotes.

The picture below represents an oxygen atom.
How many protons does this
atom
have?

Answers

First answer: A : 8 protons







Hope this answers your question

The number of protons in this atom of oxygen is 8. Thus, the correct option is A.

What are Protons?

Protons may be defined as stable subatomic particles present in the nucleus of every atom. It is positively charged in nature.

The number of protons of an atom is equal to its atomic number. Oxygen has an atomic number of 8, which means the number of protons is equal to 8 as well.

Therefore, it is well described above.

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Chlorpromazine (Thorazine) may cause which of the following side effects? a. Respiratory depression b. Hemorrhage c. Tachycardia d. Gastric ulcers

Answers

Answer:

The correct answer is option d. "Gastric ulcers".

Explanation:

Chlorpromazine (Thorazine) is an antipsychotic medication, the widest medicine used to treat schizophrenia, and other psychotic disorders such as bipolar disorder. One of the most common side effects of the use of Chlorpromazine is the formation of gastric ulcers. This happens because Chlorpromazine inhibits histamine and insulin, two compounds that stimulate gastric secretion.

How can plant crops be modified so that they do not need to be sprayed with pesticides that are also harmful to humans like normal crops are?
A. They can be given DNA to allow them to resist harsh conditions.
B. They can be given human DNA so that the pests will not want to eat them.
C. They cannot be modified to be better than organic crops
D. They can be given bacterial DNA to produce a toxin that only works on bugs and other pests.

Answers

I think the answer is D ::/://)(;,/;

Genetically modified plants are engineered and have inbuilt resistance toward pests, insects, etc. These plants are introduced with bacterial DNA that produces toxins that kill pests. Thus, option D is correct.

What are genetically modified crops?

Genetically modified plants are made through the process of genetic engineering and recombinant technology by introducing the modified DNA or genetic material into the host to confer properties.

Genetically modified (GM) crops have been known to have traits beneficial to the plant to provide resistance against insects, pests, diseases, chemicals, the environment, etc.

The plant is introduced to the modified and engineered bacterial genome that releases toxic substances that can kill insects, rodents, pests, arthropods, etc.

Therefore, option D. the genetically modified plants produce a toxin that kills pests.

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Jasmin left a towel to dry overnigjt,explain why the wet towel was able to dry even though theres no sun

Answers

Answer:

When water is warm it evaporates much quicker than when water is cool. Therefore, the heat from the sun helps to dry clothes quickly.

Wind - Another drying factor is the wind. If it is a windy day the water is evaporated more quickly due to the air moving / passing through the clothes.

Try this outside experiment on a windy day and compare your results to doing this experiment on a sunny day.

Be sure to do a fair test using the same items and same method.

So, to sum it up, why do clothes dry in the sun?

Wet clothes dry in sunshine because the water that makes them wet evaporates. This means that the water turns into a gas and becomes part of the air. Evaporation happens more quickly when water is warm, and this is why a warm day is better than a cool day for drying clothes. Evaporation happens more quickly if the air is moving as well - so windy days are good days to help dry your clothes quicker.

What is one characteristic that you could observe to distinguish diatoms and dinoflagellates

Answers

Answer: Diatoms have a cell wall that includes silica, while dinoflagellates have a cell wall that includes cellulose.

Explanation:

Diatoms are unicellular algae. Some of them can live in colonies forming filaments or ribbons, they are surrounded by a cell wall made of silica called a frustula.

They are microscopic animals, almost always single-celled. They have flagella. They can be divided into two large groups differentiated by the presence or absence of cellulosic plaques in the cell wall or amphysm.

A student investigated whether ants dig more tunnels in the light or in the dark. She thought that ants used the filtered light that penetrated the upper layers of earth and would dig more tunnels during the daytime. Ten ant colonies were set up in commercial ant farms with the same number and type of ants per ant farm. The same amount of food was given to each colony, and the colonies were in the same temperature. Five of the colonies were exposed to normal room light and five were covered with black construction paper so they did not receive light. Every other day for three weeks the length of the tunnels was measured in millimeter using a string and a ruler. Averages for the light and dark groups for I each measured were then computed. The averages are listed in the following chart.

Length of Tunnels(mm) constructed by Ants in different Light conditions
Day. Light. Dark
1 5 7
3 10 15
5 20 25
7 26 32
9 32 47
11 50 62
13 61 93
15 66 110
17 90 115
19 95 120
21 103 136

Q1. List all materials needed to do this experiment.


Q2. Promblem:




Q3. Hypotheses:



Q4. Write a support or non-support of hypotheses.




Q5. Independent Value:




Q6. Dependent Value:




Q7. Is there a control? If so...what is it?



Q8. Sources of Experimental Error:​

Answers

Answer:

Please see below

Explanation:

Source of light, measuring tool (the string and ruler), ant food, ant farmsWhether or not the tunnels created by ants differ in length depending on the amount of light availableMore tunnels are dug by ants during the daylight hoursThe hypotheses will probably come out to be true since ants are usually hiding away from predators during the nightThe presence of light The length of a particular tunnelPerform a trial under normal conditions (room light)Inaccuracy or inconsistency in measurements

How does blood ph change during exercise?

Answers

Answer:

The lungs provide a faster way to help control the pH of the blood. In response to exercise, the body increases its breathing rate which helps to counteract the pH-lowering effects of exercise by removing CO2, a component of the principal pH buffer in the blood.

1. What pathway is used to make glucose from other metabolites such as oxaloacetate?​

Answers

The Kreb cycle or also known as the citric acid cycle makes oxaloacetate

Charles Darwin and Alfred Wallace first suggested: A. The idea that evolution might occur. B. A theory to explain how evolution occurs within populations. C. Changes to an individual organism’s body, caused by changes in the environment, might be passed on to the organism's offspring. D. None of the above.

Answers

Answer:

You watched the lab demo video and looked over the lab sheet.  You saw how the catalyst (MnO2) affected the rate of reaction as hydrogen peroxide (H2O2) broke down in to water (H2O) and oxygen gas (O2).

How did the catalyst affect the rate of reaction?

What was the evidence that the catalyst was having that affect?

Explanation:

The words below are monomers of what Carbo Hydrates, Proteins, Lipids, or Nucleic Acids 1- Glucose 2- phosphate 3- Sugar (5 C) 4- Amino Acid 5-Glycerol 6-Nitrogenous Base

Answers

Answer:

Explanation:

glucose is the monomer of carbohydrate

phosphate is the monomer of nucleic acid

sugar is the monomer of nucleic acid

amino acid is the monomer of protein

glycerol is the monomer of lipid

nitrogenous base is the monomer of nucleic acid

What effect do the 4 zones of the tropical rainforest have? ​

Answers

Most rainforests are structured in four layers: emergent, canopy, understory, and forest floor. Each layer has unique characteristics based on differing levels of water, sunlight, and air circulation.

Answer:

Most rainforests are structured in four layers: emergent, canopy, understory, and forest floor. Each layer has unique characteristics based on differing levels of water, sunlight, and air circulation.

Explanation:

How would you determine the volume of a dead cat

Answers

Answer:

most cats are about approximately 648 cubic inches in volume

uh

come again?...........

Trees grow throughout the year. During which season(s) do you think trees grow fastest?

Answers

trees grow the most in late spring through very early summer. many trees experience another smaller growth spurt in early fall so i think most around spring and summer that helps nutrients the roots more on the spring and summer side

True statement about a container of a gas

Answers

Answer:

An ideal gas differs from a real gas in that the molecules of an ideal gas have no attraction for one another

Explanation:

Which of the following is a piece of empirical evidence supporting evolution? a. Biogeorgraphy b. Mycology c. Asexual reporduction d. progression of traits

Answers

Answer:

The answer is Biogeography

Answer:

Biogeography

Explanation:

Correct

Numerous reasons provide evidence for the theory of evolution. These include biogeography, vestigial structures, and DNA. It does not include mycology, asexual reproduction, or progression of traits.

g Organisms with variations that make them best suited to their environment are more likely to _____.

Answers

Answer:

Organisms with variations that makes best suited to their environment are more likely to survive.

(Darwin's theory)

atmospheric nitrogen is converted to compounds of Nitrogen in the soil by dash dash​

Answers

My answer is Nitrogen Fixation.

Angela is curious about how Clomid works to stimulate her ovaries. Her doctor told her that this drug works by "tricking the brain into thinking that the levels of estrogen in the body are low." Which of the following statements help explain why this would lead to a stimulation of the ovaries?
a. Clomid is a drug that inhibits the effect of FSH stimulating follicle development in the ovaries
b. Clomid prevents estrogen from exerting negative feedback, so LH and FSH levels remain high and continue to stimulate the ovaries
c. Clomid produces strong negative feedback on the brain, which leads to a drop in FSH and LH levels.
d. Clomid blocks the effect of FSH on the ovaries. The ovaries respond by increasing their production of estrogen.

Answers

Answer:

b. Clomid prevents estrogen from exerting negative feedback, so LH and FSH levels remain high and continue to stimulate the ovaries

Explanation:

Clomifene, also known by its commercial name Clomid, is a drug used to stimulate ovulation, and therefore used in fertility treatment. Clomid is a drug that works on estrogen receptors in order to stimulate the secretion of gonadotrophin by the pituitary gland, and by blocking negative feedback of estrogen steroid hormones (estradiol), thereby increasing the concentration of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). A high FSH concentration leads to the growth of ovarian follicles, whose rupture results in ovulation.

Explain how and where volcanoes form (how the chemical composition of lava affects the type of volcano formed).

Answers

Answer:

Magmas differ in composition, which affects viscosity. Magma composition has a large effect on how a volcano erupts. Felsic lavas are more viscous and erupt explosively or do not erupt. Mafic lavas are less viscous and erupt effusively.

Explanation:


How could you adapt the
apparatus shown in Figure 1.9
to find out:
a) if other animals produce
carbon dioxide
b) if plants produce carbon
dioxide?

Answers

Answer:

with the presence of oxygen

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ABC Inc. manufactures bedding sets. The budgeted production is for 55,000 comforters in 2012. Each comforter requires 7 yards of material. So 385,000 yards will be needed. The estimated January 1, 2012, beginning inventory is $124,000 in material. The desired ending balance is $120,000 in material. If the material costs $4.00 per yard, determine the direct materials budget for 2012. Which of the following is one of the most abundant elements in all livingthings?A. GoldB. HeliumC. OxygenD. Silver pls help ASAPwhich of these is an approximation of 7/9 to the nearest hundred A) 0.078 B) 0.78 C) 1.29 D) 7.8 Need help I dont understand Last week, Harry drank too much tequila, and doing so made him vomit. Now just the smell of tequila makes his stomach a bit queasy. Vomiting after drinking too much tequila is an example of a: ________ You observe the growth of a group of plants that have been tipped over. How will auxin influence cell growth in the roots of these plants? (1 point) Auxin will build up on the upper side of the root and inhibit cell growth. Auxin will build up on the lower side of the root and stimulate cell growth. Auxin will build up on the lower side of the root and inhibit cell growth. Auxin will build up on the upper side of the root and stimulate cell growth. Will give brainliest !! What are the five key things you must know in order to identify opportunities in your area of work? Help please!!!!! Thank you Drag each tile to the correct category Match each description to a characteristic of business writing Mathematical models are used in analyzing population rate Please select the best answer from the choices provided true false A map has a scale of 1cm:20 km two cities are 2.5 cm apart on the map to the nearest tenth of a kilometer what is the actual distance corresponding to the map distance The radius of a sphere-shaped balloon increases at a rate of 2 centimeters (cm) per second. If the surface area ofthe completely inflated balloon is 784 cm, how long will it take for the balloon to fully inflate?Use SA=4r27 seconds10 seconds49 seconds 196 seconds Planes X and Y and points J, K, L, M, and N areshown.Exactly how many planes contain points J, K, and N?A) 0B) 1C) 2D) 3 A parenthetical element is a (5 points)part of a sentence that can be removed without changing the meaning of the sentencegroup of words that connects other words, phrases, dependent clauses, and sentencesphrase or clause containing three or more words at the beginning or end of a sentenceportion of a sentence that is put into parentheses to show its importance in the sentence "The time horizon to be used when constructing a portfolio to pay for college expenses for a person who is expected to start college in 10 years and finish college in 15 years is:" Tell whether each equation is True, False, or Open. Explain. "must explain for credit"1. 6-t=122. 7+(-5)=123. 31 - 15 = 22 + (-3)2 Scientists want to place a telescope on the moon to improve their view of distant planets. The telescope weighs 200 pounds on Earth. What will happen to the weight of the telescope when it reaches the moon? The weight of the telescope will increase. The weight of the telescope will decrease. The weight of the telescope will stay the same. The weight of the telescope will be equal to its mass. You have a multi-domain Windows Server 2016 forest. You need to make a shared folder available to users from several domains. What type of group should you create to add the users to and assign permissions to the resource? Ellie is doing research for a paper on the government of ancient Greece. She knows little about the topic, so shewants to read something that will help her figure out what questions to ask.Which text would best match her reading purpose?O "The Ancient Ruins of Greece," an advertisement on a travel websiteO "The Pillars of Greece," a timeline with pictures that show key events in ancient Greek historyO "Ancient Greek People and Their Rulers," an article in an academic encyclopediaO "From Ancient Greece to Today," a blog written by a college student for a political science class Evaluate the integral. W (x2 y2) dx dy dz; W is the pyramid with top vertex at (0, 0, 1) and base vertices at (0, 0, 0), (1, 0, 0), (0, 1, 0), and (1, 1, 0)