You have a circular plasmid containing 9 kb of DNA, and you wish to map its EcoRI and BamHI sites. When you digest the plasmid with EcoRI and run the DNA on a gel, you observe a single band at 9 kb. You get the same result when you digest the DNA with BamHI. When you digest with a mixture of both enzymes, you observe two bands, one 6 kb and the other 3 kb in size. Explain these results. Draw a map of the restriction sites.
Answers
Answer:
A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both
Explanation:
Circular plasmid containing : 9 kb of DNA
Eco R1 and Bam H1 are known as restriction sites in DNA ( i.e. special sequences found in a DNA ) hence they can be specifically cleaved by the restriction enzymes enabling the insertion of a gene. that is why you will get a same result when you digest the Plasmid with either Eco R1 or Bam H1
but A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both
Related Questions
Describe how the movement of water landforms form
Answers
Answer:
The moving of water causes erosion, deposition and much more!
Explanation:
Water moving across the earth in streams and rivers pushes along soil and breaks down pieces of rock in a process called erosion. The moving water carries away rock and soil from some areas and deposits them in other areas, creating new landforms or changing the course of a stream or river.
In cats, the gene for Calico (multicolored) cats is an x-linked trait that is
CODOMINANT. Females that receive a "B" and an "R" gene have black and orange
splotches on white coats. Males can only be black or orange, but never calico.
A female calico's genotype is XBXR
Cross a female calico cat with a black male. (Complete a Punnett square)
What are the results of the cross?
25% Black and male, 0% Calico Male, 25% Calico female
50% Black and male, 50% Calico female.
50% Orange and male, 50% Female Black
Answers
Answer:
Considering the whole progeny (100%), there will be
25% Black male kittens, XBY0% Calico Male kittens25% Calico female kittens, XBXRExplanation:
Available data:
The gene for Calico (multicolored) cats is an x-linked trait and codominantCalico Females receive a "B" and an "R" gene, and have black and orange splotches on white coats. Their genotype is XBXR.Males can only be black or orange, but never calico. Their genotype is XBY and XRYCross: a female calico cat with a black male
Parentals) XBXR x XBY
Gametes) XB XR XB Y
Punnett square) XB XR
XB XBXB XBXR
Y XBY XRY
F1) Among the whole progeny:
2/4 = 50% will be black (female XBXB and male XBY) 1/4 = 25% will be Calico (female XBXR) 1/4 = 25% will be Orange (male XRY)Among females:
1/2 = 50% of females will be black, XBXB 1/2 = 50% of females will be Calico, XBXRAmong males:
1/2 = 50% of males will be black, XBY 1/2 = 50% of males will be orange, XRYWhich is NOT a part of cell theory?
A. The basic unit of life is a cell.
B. All cells are produced from other living cells.
C. All cells need light to live.
D. All organisms are made of cell.
Answers
Explanation: I hope this helps you !
The image shows a cell placed in an aquatic environment. Using your
knowledge of water tonicity, what type of environment was the cell placed
in, and what effect will this have on the cell?
The environment outside of the cell is hypertonic, and the cell will swell.
The environment outside of the cell is hypertonic, and the cell will shrink.
The environment outside of the cell is hypotonic, and the cell will swell.
The environment outside of the cell is hypotonic, and the cell will shrink.
Answers
Answer:
the water will go out of the cell
Explanation:
You have been studying two Hippogriff traits as a senior graduation project at Hogwarts (because you are magical), beak size (big beaks, B, are dominant to small beaks, b) and feather color (brown feathers, F, are dominant to white feathers, f). You mate two brown, big-beaked Hippogriffs and obtain the following results: 15 of the baby Griffs are brown and big-beaked; 4 of the baby Griffs are white are big-beaked; and 1 of the baby Griffs is white and small-beaked. What are the genotypes of the parent Hippogriffs
Answers
Answer:
BbFf is the genotype of parent Hippogriffs
Explanation:
Given -
Big beaks, B, are dominant to small beaks, b.
Brown feathers, F, are dominant to white feathers, f.
Two brown, big-beaked Hippogriffs are mated to produce 15 brown and big-beaked; 4 white and big-beaked; and 1 white and small-beaked offspring
The genotype of parents is BbFf
Pls someone help its a very easy question also don’t worry about the dot I accidentally did that
Answers
DeShawn wants to conduct a scientific study on how land-clearing in his city may have affected the quality of the water. Which source of information would be most valuable to his research? A. a survey given to the people of the city ten years ago on the possible effects of land-clearing on water B. the opinion of a neighbor who has some knowledge on how to test city water for impurities C. a recent city government report with data regarding how clean the water supply is D. a recent political article stating that the water of the city should be filtered and cleaned Reset Next Question
Answers
Answer: i think that the answer is C
Explanation:
The source of the information that should be most valuable to the research should be option C.
What is research?
It means that the development of the new knowledge should be done or there is the usage of the existing knowledge for generating the new conceptsm methods, and understanding. It involved the synthesis and the analysis of last research for resulting the new or the creative outcomes.
So based on this, the option c should be correct.
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Select the term that best fits the following definition: Tells what elements
a compound contains and the number of those atoms *
O Covalent Bond
O lonic Bond
O Compound
O Chemical Formula
Answers
What does the term "feedback inhibition” describe?
Answers
Answer:
Feedback inhibition, in enzymology, suppression of the activity of an enzyme, participating in a sequence of reactions by which a substance is synthesized, by a product of that sequence.
In a separate study, 68 rock pocket mice were collected from four different, widely separated areas of dark lava rock. One collecting site was in Sonora, Mexico. The other three were in Chihuahua, Mexico. Dr. Nachman and colleagues observed no significant differences in the color of the rocks in the four locations sampled. However, the dark-colored mice from the three Chihuahua locations were slightly darker than the dark-colored mice from the Sonora population. The entire Mc1r gene was sequenced in all 68 of the mice collected. The mutations responsible for the dark fur color in the Sonora mice were absent from the three different populations of Chihuahua mice. No Mc1r mutations were associated with dark fur color in the Chihuahua populations. These findings suggest that adaptive dark coloration has occurred at least twice in the rock pocket mouse and that these similar phenotypic changes have different genetic bases.
Answers
Complete question:
In a separate study, 68 rock pocket mice were collected from four different, widely separated areas of dark lava rock. One collecting site was in Sonora, Mexico. The other three were in Chihuahua, Mexico. Dr. Nachman and colleagues observed no significant differences in the color of the rocks in the four locations sampled. However, the dark-colored mice from the three Chihuahua locations were slightly darker than the dark-colored mice from the Sonora population. The entire Mc1r gene was sequenced in all 68 of the mice collected. The mutations responsible for the dark fur color in the Sonora mice were absent from the three different populations of Chihuahua mice. No Mc1r mutations were associated with dark fur color in the Chihuahua populations. These findings suggest that adaptive dark coloration has occurred at least twice in the rock pocket mouse and that these similar phenotypic changes have different genetic bases.
How does this study support the concept that natural selection is not random?
Answer:
The study supports the concept that natural selection is not random because in different areas with the same or very similar environmental characteristics, the same phenotype was produced by different types of mutations.
Explanation:
All of the sampled animals are inhabiting dark substrate. Probably animals needed to camouflage to survive. Natural selection must have driven them to produce dark color, similar to the substrate color. So animals from the different regions suffered different mutations that drove them to have almost the same dark fur color. The environmental condition is favoring the same phenotype.
A solution is modified in doing so the pH changes from 8 to 4. This will make the actual Concentration of hydrogen ions Change by a factor of?
A.1000
B.100
C.10000
D.4
E.10
Answers
how and why does DNA change during meiosis
Answers
Answer:
When recombination occurs during meiosis, the cell's homologous chromosomes line up extremely close to one another. Then, the DNA strand within each chromosome breaks in the exact same location, leaving two free ends. Each end then crosses over into the other chromosome and forms a connection called a chiasma.
Explanation:
help! If a virus has an incubation period of 12-24 months, then it is most likely --
Answers
my best guess would be LTBI (latent tuberculosis infection)
most incubation periods are in the range of 3 weeks to 3 months, though some cancer viruses have incubation periods of 10 years long. exc..
tuberculosis: 6-24 months
aids: 1-15 years
leukemia: 2–12 years
good luck!
in drosophila melanogaster, the common fruit fly, curved wings "I" and purple eyes "r" are two linked recessive genes found on chromosomes 2. The dominant wild type alleles for those genes are long wings"L" and red eyes "R"
a)describe the expected ratio of offspring in the f2 generation of these genes were located on different chromosomes
b)the results in the f2 generation of these flies were: 40 long winged, red eyes; 40 curved winged, red eyes; 10 long winged, purple eyes, and 10 curved winged, red eyed. how many map units apart are the genes for curved wings and purple eye color?
Answers
Answer:
the other person is right, he had messed up before saying that 60 x 40 = 100, but 60 + 40 = 100. Then I had told him that his answer was wrong and that he should put the answer as 240 instead since 60 x 40 = 240 and not 100.
Explanation:
The expected ratio of offspring in the f2 generation of these genes were located on different chromosomes is 25%
What are chromosomes?A chromosome is a long DNA molecule with part or all of the genetic material of an organism. In most chromosomes the very long thin DNA fibers are coated with packaging proteins; in eukaryotic cells the most important of these proteins are the histones.
Chromosomes allow DNA to be accurately copied during these cell divisions. So one more time. Chromosomes are found in the nuclei of our cells and allow DNA to be accurately copied during cell division.
Chromosomes are thread-like structures located inside the nucleus of animal and plant cells. Each chromosome is made of protein and a single molecule of deoxyribonucleic acid (DNA).
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Which word describes the process by which a human transmits a disease to an animal?
Answers
Answer: reverse zoonosis or anthroponosis.
Explanation:
Answer:
a zoonosis
Explanation:
is an infectious disease caused by a pathogen (an infectious agent, such as a bacterium, virus, parasite or prion) that has jumped from an animal (usually a vertebrate) to a human. Typically, the first infected human transmits the infectious agent to at least one other human, who, in turn, infects others
What is the optimal pH that this enzyme functions at? And What is the optimal temperature that this enzyme functions at?
Answers
Answer:
Optimum Temperature is 35°C
Optimum PH is 7.0.
Explanation:
The optimum temp of enzymes is 20-35°c. They become inactivated at very low temperature and denatured (destroyed) at very high temp i.e. greater than 45°C. Low molecular weight enzymes are comparatively more heat stable.
The optimum pH of most enzymes is pH 7.0 (neutral pH).
What factor causes global wind patterns
Answers
Answer:
the Coriolis effect
Explanation:
the earths rotation causes wind also known as the coriolis effect
Answer:
unequal heating of Earth's surface by the Sun
Explanation:
What is the transfer of
heat from one object to
another through direct
contact?
Answers
Answer:
condiction
Explanation:
conduction is the transfer of heat between substances that are in direct contact with each other.
Match the body systems and interaction descriptions:
1. Circulatory and Respiratory
2. Digestive and Circulatory
3. Nervous, Muscular, and Skeletal
4. Circulatory and Integumentary
5. Muscular and Digestive
6. Respiratory and Muscular
7. Circulatory and Skeletal
8. Excretory and Circulatory
9. Nervous and Integumentary
10. Muscular and Circulatory
_. Nutrients pass into the circulatory system to be carried to body cells
_. Lungs supply oxygen carried by blood to cells of the body
_. Production of blood cells in bone marrow
_. Chewing, swallowing and movement of food through the digestive tract
_. Pumping of the heart & blood
_. Daily movement and coordination
_. Kidneys remove wastes from blood
_. Cooling through dilation of blood vessels near the surface of the skin
_. Sense of touch
_. Movement of the diaphragm in breathing
Answers
2. Nutrients pass into the circulatory system to be carried to body cells
1. Lungs supply oxygen carried by blood to cells of the body
7. Production of blood cells in bone marrow
5. Chewing, swallowing and movement of food through the digestive tract
6. Pumping of the heart & blood
3. Daily movement and coordination
8. Kidneys remove wastes from blood
4. Cooling through dilation of blood vessels near the surface of the skin
9. Sense of touch
10. Movement of the diaphragm in breathing
There are numerous parts to the human body. Every physical portion serves a distinct purpose.
What is body systems and their interaction?There are numerous parts to the human body. Every physical portion serves a distinct purpose. To better comprehend how the human body's components interact with one another and work together as a whole, scientists divide the body into systems. A group of bodily parts that cooperate to carry out a specific task is known as a body system.
For instance, the integumentary system, that shields the body from its surroundings, is made up of the skin, hair, and nails. Another instance of an organ that guards the body is the immune system.
1. Lungs supply oxygen carried by blood to cells of the body
2. Nutrients pass into the circulatory system to be carried to body cells
3. Daily movement and coordination
4. Cooling through dilation of blood vessels near the surface of the skin
5. Chewing, swallowing and movement of food through the digestive tract
6. Pumping of the heart & blood
7. Production of blood cells in bone marrow
8. Kidneys remove wastes from blood
9. Sense of touch
10. Movement of the diaphragm in breathing
Therefore, there are numerous parts to the human body. Every physical portion serves a distinct purpose.
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Which of the following molecules comes from the food we eat and is needed for energy?
A) amino acids
B) glucose
C) oxygen
D) protein
E) fiber
Answers
I NEED HELP ON BOTH ANSWER AS QUICKLY AS POSSIBLE!!
Bc
Answers
Answer:
What do you need help with?
What would happen to Earth's climate if we had no moon, and why?
Answers
Answer:
The tilt creates what we know as seasons and changing weather. Without the pull of the moon, this tilt would change.
Explanation:
Either the tilt would become extreme, leading to extreme and severe seasons, or the tilt would decrease, and we would see almost no seasons at all. The moon influences life as we know it on Earth.
Answer: Because It is the pull of the Moon's gravity on the Earth that holds our planet in place. Without the Moon stabilising our tilt, it is possible that the Earth's tilt could vary wildly. It would move from no tilt (which means no seasons) to a large tilt (which means extreme weather and even ice ages).
The tilt creates what we know as seasons and changing weather. Without the pull of the moon, this tilt would change. Either the tilt would become extreme, leading to extreme and severe seasons, or the tilt would decrease, and we would see almost no seasons at all. The moon influences life as we know it on Earth. Hope this helps have a awesome day/night❤️✨Explanation:
Which term indicates that a single gene has two or more alleles present in a population?
A. Genotype
B. Point mutation
C. Polymorphism
D. Pleiotrophy
Answers
Genetic polymorphism is the existence of multiple alleles of a single gene.
What are genes?Genes are regions of DNA which code for a particular protein or any other gene product.
Genes can exists as a single copy or as multiple copies.
Alleles are alternate forms of a particular gene.
A single gene that has two or more alleles present in a population is known as genetic polymorphism.
Therefore, multiple alleles of a single gene is known as genetic polymorphism.
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Perhaps no one cares more about being able to see in the ocean than do divers. The Cayman Islands, Bonaire and the Bahamas are all popular dive destinations due to both submarine features, such as coral reefs, and water clarity. Under the best of circumstances, visibility in these locations is between 50 ft (15 m) to 150 ft (45 m). Based on this, and what you have learned about sound, write a paragraph describing those ways in which humans and marine organisms use sound for some underwater tasks and light for others.
Answers
Answer:
Humans and marine organisms use sound finding depth of the ocean and objects.
Explanation:
Humans and marine organisms use sound for some underwater tasks such as finding depth of the ocean and objects. Human use sonar which is a device used to explore the ocean and animals use echolocation to find food and identify objects that comes in their way. Sonar is present in submarine uses sound waves to detect objects that comes in their way so both humans and marine organisms use sounds.
name two characteristics that all these animal share ( frog, buck, doves, lizard, dragonflies )
Answers
Answer:
They all have a beating heart, and a brain
Explanation:
Beating the heart and a brain is the two characteristics that all these animal shares frog, buck, doves, lizard, and dragonflies.
What are the different characteristics of animals?Frogs are cold-blooded animals and are classified into the class of amphibians, they are vertebrate animals with backbones.
The lizard was included in the class reptilia, they are creeping and burrowing terrestrial animals situated on the land, and also have scales on their body. Similar to the frog, they are also cold-blooded animals having a backbone.
The major classification of the animals is done by the presence of a backbone, which differentiates them in vertebrates and invertebrates.
Therefore, in the given question all the animals share common feature of having a heart and brain.
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What is a mold? in fossils
Answers
The temperature of the earth's surface during different seasons is also affected by
the passage of the sun's rays through the atmosphere.
True
False
Answers
Answer:
True
Explanation:
The sun's light rays produce heat which is actually the only reason why we are not living in a block of ice.
Winter is when we are farther from the sun thus it is colder than the summer when we absorb more of the sun's rays.
The statement "the temperature of the earth's surface during different seasons is also affected by the passage of the sun's rays through the atmosphere" is definitely true.
What is an Atmosphere?An atmosphere may be characterized as a type of layer that significantly consists of a mixture of numerous gases that surrounds any celestial body or planet like the earth, mars, etc. The atmosphere of the earth is composed of about 78% nitrogen, 21% oxygen, and one percent other gases.
The rays of the sun typically produce a significant amount of heat. Due to this, we are not living in a block of ice and get a source of energy directly from the sun.
During winter, the sun is farther from the surface of the sun, so we receive less amount or indirect light but in summer, we absorb more of the sun's rays. Due to this, the temperature increases and we feel extremely hot outside.
Therefore, the statement "the temperature of the earth's surface during different seasons is also affected by the passage of the sun's rays through the atmosphere" is definitely true.
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The first stage of the cell cycle during which the cell matures and prepares to divide and copies
its DNA; The stage the cell stays in the most?
Answers
The cell multiplies and creates a copy of its DNA during interphase. In the mitotic (M) phase, the cell divides into two new cells by dividing its cytoplasm and DNA into two sets.
What is the main role of interphase stage of cell cycle?A cell consumes the bulk of its time in what is known as interphase, where it creates, duplicates its chromosomes, and acquires available to split. The cell then moves interphase, goes direct mitosis, and completes dividing.
The cell multiplies and the nuclear DNA is duplicated during interphase. The mitotic phase immediately follows interphase.
Chromosomes that have been duplicated are separated and distributed across daughter nuclei during the mitotic phase. Usually, the cytoplasm is divided as well, producing two daughter cells.
Therefore, The first stage and the stage the cell stays in most is Interphase.
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URGENTTT
Which of the following best describes how independent assortment results in inherited variations within a species and how it contributes to evolution?
A Independent assortment results from the copying of DNA during cell division and causes variations that are sometimes beneficial to a species
B Independent assortment results from the binary fission of a single cell and causes mutations that can be beneficial or harmful to a species
C Independent assortment occurs during mitosis and causes uncontrolled cell division which is harmful to a species
D Independent assortment occurs when chromosomes separate during meiosis and causes variations that can be beneficial or harmful to a species
Answers
Answer:
D Independent assortment occurs when chromosomes separate during meiosis and causes variations that can be beneficial or harmful to a species
Explanation:
During meiosis, the homologous chromosomes separate and segregate independently of each other. This process is referred to as 'independent assortment'. In consequence, different gene variants or 'alleles' localized in homologous chromosomes segregate independently of each other during gamete formation, thereby one particular gamete has an equal probability to carry either of these segregating alleles. Independent assortment generates genetic variation in the gametes, which can be beneficial or deleterious in a given environment.
The option that best describes how independent assortment results is D Independent assortment occurs when chromosomes separate during meiosis and causes variations that can be beneficial or harmful to a species
What is independent assortment ?Independent assortment is a phenomenon that occurs during meiosis, which is the process of cell division that produces gametes (sex cells). During meiosis, the chromosomes are randomly sorted into the gametes. This means that the alleles (different forms of a gene) on different chromosomes can be inherited together or separately.
This random assortment of alleles can lead to new combinations of traits, which can be beneficial or harmful to a species.
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Helppppppppppppppppppppppppp
Answers
Answer:
agriculture
Explanation:
Slash-and-burn agriculture, method of cultivation in which forests are burned and cleared for planting. Slash-and-burn agriculture is often used by tropical-forest root-crop farmers in various parts of the world and by dry-rice cultivators of the forested hill country of Southeast Asia