You created three different derived strains from E.coli K12. One strain includes a deletion in a gene critical for biosynthesis of diaminopimelic acid, one strain lacks dnaQ, and the last strain lacks both. Describe experiments to clearly differentiate between these three strains through phenotypic assays alone (not just by sequencing the genomes or any derivative of genotyping). Include all necessary controls to interpert the experiment.

Answers

Answer 1

Answer:

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

Explanation:

One of the experiments that can be used to differentiate diaminopimelic acid is by observing the cell walls in which they are present because  are needed in the formation of cell walls. while to differentiate the three strains

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.


Related Questions

Calculation, contemplation, and cognition are human activities associated with increased activity in the

A) thalamus
B) hypothalamus
C) cerebrum
D) cerebellum

Answers

Answer:

C

Explanation:

found answer from "Bio-Chapter 49" quizlet-

https://quizlet.com/10652308/bio-chapter-49-flash-cards/

Calculation, contemplation, and cognition are the human activities associated with increased activity in the cerebrum. So, option C is correct.

What do you mean by Contemplation?

Contemplation may be defined as studying or observing something carefully or thinking deeply about something.

The cerebrum is the largest part of the brain. It initiates and coordinates movements and regulates temperature. It also maintains and enables the thinking, reasoning, and problem-solving activities of humans.

Therefore, Calculation, contemplation, and cognition are the human activities associated with increased activity in the cerebrum.

To learn more about the Human brain, refer to the link:

https://brainly.com/question/26043723

Where do substrates bind on an enzyme?
active site
surface receptor
voltage-gated
channels
calcium channels

Answers

The answer is active site, considering that is where the catalytic action happens.

The black panther phenotype in jaguars, which is a completely black coat, is thought to be determined by a single gene. If the presence of this phenotype were unrelated to habitat type, but was found to be much more common in populations in Costa Rica relative to populations in Brazil, what would be the most likely explanation for this geographic pattern

Answers

Answer:

Due to suitable climate and food availability.

Explanation:

The reason for this geographical distribution is the availability of suitable climate and food. Black panthers live in the hot, dense tropical rainforests environment where there is a lot of food present that is necessary for sustaining the population of black panthers. Black panthers are more common in Costa Rica as compared to populations in Brazil due to climate as well as food availability.

escribe en cual de los organos anteriores tienen lugar estos procesos . se filtran las sustancias de desechos que ha recogido la sangre​

Answers

Answer:

Riñones.

Explanation:

Los riñones son ​​dos órganos ubicados en la cavidad abdominal detrás del peritoneo, a la izquierda y derecha de la columna. Los riñones tienen la función de mantener constante la composición de la sangre, eliminando así las sustancias no deseadas disueltas, como los productos de desecho del metabolismo y los venenos y medicamentos absorbidos a través de los alimentos. El riñón también mantiene el equilibrio ácido del cuerpo a largo plazo, mediante el transporte de carbonato de hidrógeno e iones de hidrógeno. El producto que producen los riñones, una solución de sustancias que el cuerpo ya no puede usar, se llama orina.

In Journey to the Center of the Earth, by Jules Verne, an adventurer travels through the layers of Earth until he reaches the center. While it is not possible to actually travel through all of the layers of Earth, in what order would the adventurer have traveled to reach the center? Write the layers (outer core, mantle, inner core, crust) in the correct order from outermost to innermost layer.

Answers

Answer:

The correct answer would be - crust, mantle, outermost and innermost layer.

Explanation:

It is not possible to travel through all of the layers of Earth, however, Hypothetically, if an individual takes a journey of traveling to the core of the Earth, he needs to go through total five total layers, including the "center of the Earth" as described in Jules Verne's novel.

The crust is the first and outermost layer of the earth from the top of the earth's surface. It is the layer we and all geographical areas such as caves present. The second layer is the mantle that can be distinguished in the upper mantle is the primary mantle itself. After the mantle person would enter the outer core and then the final layer is the inner core.

Can someone help me with this?

Answers

Answer:

Plants and animals share many characteristics, but they are different in some respects. Animals usually move around and find their own food, while plants are usually immobile and create their food via photosynthesis. ... Animal cells absorb nutrients from food, while plant cells use plastids to create energy from sunlight.

Explanation:

app app
[tex] \geqslant a[/tex]

Answers

Answer:

what?

Explanation:

Huh I’m confusion, what does this mean?

PLEASE ANSWER ASAP FOR BRAINLEST!!!!!!!!!!!!!!!!!!!!!!!!!!
A cross between a black cat & a tan cat produces a tabby pattern (black & tan fur together). What pattern of inheritence does this illustrate?
A. Codominance, two phenotypes together at the same time.
B. Incomplete , two phenotypes blend together at the same time.
C. Recessive, one phenotype is dominant and one is recessive

Answers

I’m guessing this would be C based on the fur types


- always expressed when one or two alleles are present
- only expressed when two alleles are preset

Answers

Answer:

A dominant phenotype will be expressed when at least one allele of its associated type is present, whereas a recessive phenotype will only be expressed when both alleles are of its associated type. However, there are exceptions to the way heterozygotes express themselves in the phenotype.

Explanation:

what causes the formation of ocean currents?
A: rotation of Earths on its axis
B: change in the magnetic field of Earth
C: temperature differences of water in the ocean
D: movement of tectonic plates under Earth's surface

Answers

Answer:

a

Explanation:jhgyutfyrdtsardfghygygtfydrtdrtdtyytftydtrdrdrtdxfhdy8

Answer:

It may be C or D.

Sorry if I'm wrong.

Explanation:

Petrus brings his 87-year old grandmother to the emergency department where you work. His grandmother has a fever, is dehydrated and confused, and complains of pain when urinating. Petrus reports that she has not been eating or drinking, but she has no signs or symptoms of gastrointestinal distress. You request blood samples and also a urine sample. You decide to keep Petrus' grandmother in the hospital so that she can be given intravenous fluids. You also want to wait for the lab test results before you decide whether she required additional treatment or if she can return home.
1. When Petrus' grandmother's test results are sent to you, you discover that while her blood work is normal, her urine has Gram-negative bacilli in it. Based on this, you know that the cells are:______.
a) stained blue and are cylindrical in shape.
b) stained purple and are cylindrical in shape.
c) stained pink and are spherical in shape.
d) stained pink and are cylindrical in shape.
e) stained green and are oval in shape.
2. Petrus asks you what the Gram-negative result means and you explain that the bacteria causing his grandmother's UTI:____.
a) have a thin cell wall and an outer membrane which makes the cells less sensitive to the antibiotic penicillin.
b) have a thick cell wall and an outer membrane which makes the cells less sensitive to the antibiotic penicillin.
c) have no cell wall but they have a thick outer membrane which makes the cells less sensitive to the antibiotic penicillin.
d) have a thin cell wall and an inner cell membrane which makes the cells less sensitive to penicillin.
e) have no cell wall which makes the cells more sensitive to the antibiotic penicillin.

Answers

Answer:

d) stained pink and are cylindrical in shape.

b) have a thick cell wall and an outer membrane which makes the cells less sensitive to the antibiotic penicillin.

Explanation:

Gram-negative bacteria are bacteria that do not retain the crystal violet stain. So they stain in pink and they have an outer cell membrane unlike gram positive bacteria.

Earthworms improve the texture and composition of the soil, aerating compacted earth, adding nitrogen to the soil, and helping form small clusters
of soil, called (blank)
which their slime helps hold together.

Answers

Answer: i think its soil structures

Explanation:

Worms dig hols and hold the soil togetyer with slime and make pockets for air and water. Im not 100% sure but i think it is.

Answer:

soil structure

Explanation:

just did the assignment

mark as brainliest

follow me

How do Euglena and sperm cells use flagella?

Answers

As Following:

Euglena move by a flagella , which is a elongated whip-like network that functions like a little engine. The flagella is located on the front part , and twirls in such a way as to drag the cell through the water. It is connected at an inward pocket called the pool.In sperm cells its help in movement.

What is purpose of flagella in eukaryotic cell?

Flagella in eukaryotes are used not only for Locomotion, but also for helping in phagocytosis and eukaryotic reproduction.

Thus, this is how Euglena and sperm cells use flagella.

To learn more about flagella click here:

https://brainly.com/question/905839

#SPJ2

This small structures break down materials in the cell.

Answers

Lysosomes are small structures that break down materials in the cell.

Answer:

Lysosomes

Explanation:

an organelle in the cytoplasm of eukaryotic cells containing degradative enzymes enclosed in a membrane. In other words, they break down materials in the cell.

Who benefits from globalization easy answers pls

Answers

Developing nations experience an improved general style of living thanks to globalization. According to the World Bank, extreme poverty decreased by 35% since 1990. Later, the target of the first Millennium Development Goal was to cut the 1990 poverty rate in half by 2015.
Higher Standards of Living Across the Globe
Developing nations experience an improved standard of living—thanks to globalization. According to the World Bank, extreme poverty decreased by 35% since 1990. Further, the target of the first Millennium Development Goal was to cut the 1990 poverty rate in half by 2015.

Please help me with bio

Answers

Answer: C or D

Explanation:

Enzyme and substrate concentration
4.
*RECALL: What is a substrate?

Answers

Explanation:

base on which an organism lives the soil is the substrate of most seed plants. .A substance acted upon (as by an enzyme)

Which statement DOES NOT describe a possible function of chromosomes?

Answers

Chromosomes are thread-like structures present in the nucleus, which carries genetic information from one generation to another. They play a vital role in cell division, heredity, variation, mutation, repair and regeneration.

A worm is shown in the picture. What kind of worm is it? a planarian a leech a fluke a tapeworm

Answers

Answer:

Planarian

Explanation:

The design of the planarian is gentle, flat, and saddle, and it can be jewish, grey, blue, grey, or white. Two alleviation eyespot pigmented zones light intensity, are located on the flippant, triangle curvy head.

Answer:

A(planarian)

Explanation:

I did the test on edg 2021

Select the correct answer.
How many pea plant traits did Mendel study to prove the Laws of Inheritance?

Answers

Answer:

7 pea plant traits that mendel had to study

Answer:

Seven different features

Explanation:

Mendel studied the inheritance of seven different features in peas, including height, flower color, seed color, and seed shape. To do so, he first established pea lines with two different forms of a feature, such as tall vs. short height.

The organelle that is the site of cellular respiration is the
o mitochondrion
O nucleus
o chloroplast
o ribosome

Answers

Answer:

hope this helps

Explanation:

mitochondrion

Answer:

mitochondrion

Explanation:

Plz mark brainliest thanks

Imprinting is a type of _______ behavior.
A.inherited
B.learned
C.reflex
D.instinct

Answers

Answer:

I think it is Learned

Explanation:

Hope this helps

It’s learned I think I hope this helps.

which organ of our body works the most?​

Answers

Answer:

heart and brain of our body work most

Describe the movement of the dominoes as energy is transferred through them and then compare the movement of longitudinal waves to the movement of the dominoes. Record your evidence.​

Answers

Answer:

The waves move outwards from where the rock hit. When the waves pass the wood, it bobs up and down. In the case of the falling dominoes, the movement of the dominoes was in the direction parallel to the movement of the wave, longitudinal.

Explanation:

Hope this helps :D

Question 1 (1 point)
Tubelike cells that carry food from the leaves to other parts of the plant are called?

Question 1 options:

Xlyem


Phloem


Choloropasts

Question 2 (1 point)
Liverworts are non vascular plants and grow?

Question 2 options:

On tree tops


Flat and close to the ground


Tall like trees

Question 3 (1 point)
Structures that anchor vascular plants and take in water and minerals are?

Question 3 options:

stems


leaves


roots


flowers

Question 4 (1 point)
Which of the following is not true of mosses, liverworts, and hornworts?

Question 4 options:

They are non-vascular plants.


They have true roots, stems, and leaves


They grow in moist places.


They are small and low-growing.

Question 5 (1 point)
Which is the best explanation of water transport in a non-vascular plant like the one shown below?



Question 5 options:

Water moves only in the roots.


Water moves from cell to cell.


Water moves through a system of tubes.


Water moves directly to the flowers from the roots.

Question 6 (1 point)


Looking at the diagram above. What is the function of the anther?

Question 6 options:

catches the sticky pollen


produces the pollen


becomes the fruit


passage tube for pollen

Answers

Answer:

1) xlyem

2) flat and close to the ground

3) roots

4) they have true roots, stems, and leaves

5) water moves from cell to cell

6) produces pollen

Explanation:

The answers to the following questions are as follow:

1) xylem

2) flat and close to the ground

3) roots

4) they have true roots, stems, and leaves

5) water moves from cell to cell

6) produces pollen

What are vascular plants?

Vascular plants are a group of plants that have specialized tissues for conducting water, minerals, and nutrients throughout the plant. These specialized tissues, called vascular tissue, consist of xylem and phloem.

Xylem is a type of vascular tissue that is responsible for transporting water and minerals from the roots to the rest of the plant. It consists of tubes made of dead cells with thick cell walls that form a continuous network throughout the plant.

Phloem is another type of vascular tissue that is responsible for transporting sugars and other nutrients throughout the plant. It consists of tubes made of living cells that are arranged in a circular pattern around the xylem.

Vascular plants are important because they are able to grow to larger sizes than non-vascular plants due to their ability to transport water and nutrients throughout the plant. Examples of vascular plants include trees, grasses, and flowers.

Learn more about vascular plants, here:

https://brainly.com/question/6000589

#SPJ2

Part I: Explain Ways to Improve Your Design
Imagine that you have unlimited funding and space to build your home. Describe four ways you can modify your design to improve your home’s impact on carbon dioxide emissions or biodiversity.

Answers

There are actually a lot of ways to improve your home’s impact on carbon dioxide emissions or biodiversity and I’d to give this four (4) best ways to reduce ecological footprints. Add Energy-Saving Features – like for instance install any energy-saving into your home (e.g. lamps) or you may you like to choose energy efficient appliance Encourage Energy-Saving Habits – one example is when turning off any appliances, do not just turn off the appliance but instead unplug it because all electronics used up energy even if it is a plug.  Save/Conserve Buy carbon offset – these carbon offsets mitigates owner’s greenhouse gas emissions. In these ways, we can help our mother Earth.

Mutations help in evolution because:
*

Answers

Mutations change dna which help evolution because it changes the organisms dna

You are studying ion channels and have set up model lipid vesicles containing the three following transporters:

1. a Na+ /H+ antiporter driven by the Na+ concentration gradient.
2. The common 3Na+ /2K+ pump.
3. K+ leak channels. The experiments are designed such that all three transporters are oriented in the model lipid bilayers in the same direction they would be in the plasma membrane of a cell and that concentration gradients in vitro mimic the concentration gradients experienced by cells (e.g., the extracellular Na+ concentration is similar to the concentration outside of the vesicle whereas the concentration in the lumen of the model vesicles is similar to intracellular Na+ levels).

Required:
a. Draw the arrangement and ion flow of the three types of transporters in the model membranes.
b. What do you predict will happen to the pH of the vesicle lumen if you treat the model membranes with the drug oubain that impairs function of the 3Na+/2K+ pump? Briefly explain your answer in terms of what happens with each transporter.

Answers

Answer and Explanation:

a) you will find the arrangement and ion flow of the three types of transporters in the model membranes in the attached files.

K+ leak channels are channel proteins that allow K⁺ to leave the cell in favor of its concentration gradient. It keeps a stable equilibrium by taking out K⁺ that the 3Na+ /2K+ pump brings into the cell. Any alteration in the pump leads to an alteration in the leak channel functioning. Na+ /H+ antiporter is an integral membrane protein, and a secondary transporter is responsible for the interchange of H+ and Na+ through the membrane with no use of energy. It regulates pH within the cell. It responds to the Na+ and H+ concentration gradient, determined by the 3Na+ /2K+ pump.  An alteration in the pump functioning produces a change in the Na gradient and consequently affects the antiporter interchange action. 3Na+ /2K+ pump is an integral protein that transports K+ and Na+ against the electrochemical gradient. A significant amount of energy is used to make the pump function. It brings K+ into the cell and takes Na+ out of it. It keeps the membrane potential and the electrochemical gradient constant. The pump function is associated with the leak channels and the antiporters function.

Under normal conditions, the cell interior has a lower pH than the exterior. The K+ concentration is higher in the interior, while the Na+ concentration is higher in the cell exterior. The interior of the cell is always more electronegative than the cell exterior.

b)

The drug ouabain inhibits the pump. It impedes the Na+ exit and the K+ entrance. It occurs a sharp increase in the Na+ intracellular concentration, which influences the Na+ /H+ antiporter that changes the ions´ direction, taking out Na+ and bringing in H+ to compensate the intracellular Na+ excess. There is also a decrease in the leak channel function, which does not allow K+ to leave the cell.

Whenever the pump is inhibited, there is an increase in the intracellular pH, because Na+ concentration increases in the interior.

PLEASE HELP ASAP!!!!!
Describe the founder effect.

Answers

Answer: founder effect is the reduction in genetic variation that results when a small subset of a large population is used to establish a new colony. The new population may be very different from the original population, both in terms of its genotypes and phenotypes.

Explanation:

HELLLPPPP REMEMBER IS DUE SOON !!!!! I WILL GIVE YOU A BRANLIEST !!!!!!!!!!!!!|
A scientist finds a tiny fragment of a bone too small to be classified by its appearance. The scientist studies the tiny fragment and concludes that it belonged to an ancient primate species. How was the scientist able to classify the bone?

Answers

Answer:

Through a microscope.

Explanation:

A stereozoom microscope is a handy instrument for magnifying small features on the surface of a bone.

Answer:

Through a microscope

Explanation:

I just know

Other Questions
How is the technology in 1929 similar to some technology today? on which day is Indian Navy Day celebrated helpppppp ill give brainliest answer if you know A truck traveled 144 miles on 12 gallons of diesel fuel. Atthis rate how much fuel will the truck use to go 720 miles? DESPERAGTE WILL MARK BRAINLSITRead the stanza from the poem "July."When the tangled cobweb pullsThe cornflower's cap awry,And the lilies tall lean over the wallTo bow to the butterfly,It is July.Which answer best identifies and interprets the poetic device in these lines from the stanza? the tangled cobweb pulls The cornflowers cap awryQuestion 5 options:It is an appeal to hearing, as a cap is an outdated term for a hearing aide.It is personification, as a cobweb is described as "pulling" and a cornflower is described as wearing a cap.It is a metaphor, as the cobweb actually represents the world the poet has created.Read the excerpt from "It Sifts from Leaden Sieves." It fills with Alabaster Wool The Wrinkles of the Road--- Global warming could happen without the greenhouse effectTrue or False 1. Umaalulong2. Damo3. Alipato4. Kabibe5. Ligaya Somebodyyy help ASAP A square has a triangular hole in it. What is the area of the figure? 100 in2 92.5 in2 85 in2 70 in2 Required: a. Adams Company's production cycle starts in Department A. The following information is available for July: Units Work in process, July 1 (60% complete) 71,000 Started in July 360,000 Work in process, July 31 (20% complete) 39,000 Materials are added at the beginning of the process in Department A. Using the weighted-average method, what are the equivalent units of production for materials and conversion costs for the month of July, respectively Which statements are myths about nicotine and tobacco use? Check all that apply 21m42n32p10m 21n16 5Simplify PLEASE HELP AND SHOW YOUR WORK I WILL MARK YOU BRAINLIESt If the original square is 3 in by 7 in, but then you enlarge the square to 6 in by 14 in, what percent of the smaller original square's area is the larger square's area? Please help1. Rafael stands 55 feet from the tower and uses a clinometer to measure the angle of elevation to theviewing platform as 37. How far up the tower is the viewing platform? Helpppppppppppppppp By World War II, was Georgias center of railroads and transportation. triangle ABC is similar to triangle EHG , find the measure of angle EHG . Explain how errors in DNA replication and environmental factors cause gentic variation Life's not fair, but I'm here, but I'm hereEven though your heart may tear, don't be scaredDon't be scared, don't be scared