Plants have both chloroplasts and mitochondria, while animals and fungi have only mitochondria.
Chloroplasts are responsible for photosynthesis and are found in plant cells. They are the organelles where light energy is converted into chemical energy. Mitochondria, on the other hand, are present in both plant and animal cells and are involved in cellular respiration, producing energy in the form of ATP. Fungi, like animals, do not possess chloroplasts and rely solely on mitochondria for energy production. Therefore, the statement that accurately represents the distribution of these organelles is that plants have both chloroplasts and mitochondria, while animals and fungi have only mitochondria.
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1. What phyla does this fungus belong to? 2. What type of ecosystems is this fungus located in? 3. Does this fungi provide any ecosystem services? 4. Are there any human uses or diseases caused by this fungus?
To accurately answer your questions, I would need specific information or a description about the fungus in question. Fungi belong to the kingdom Fungi, which is further classified into various phyla. There are numerous fungal species found in different ecosystems worldwide, and their ecological roles and impacts can vary significantly.
The type of ecosystem in which a fungus is located depends on the specific species. Fungi can be found in diverse habitats such as forests, grasslands, wetlands, and even in aquatic environments. They play crucial roles in nutrient cycling, decomposition, symbiotic relationships, and as primary producers in some ecosystems.
Many fungi provide important ecosystem services. For example, they play a vital role in decomposition, breaking down organic matter and recycling nutrients. Fungi also form mutualistic associations with plants, such as mycorrhizal symbiosis, aiding in nutrient uptake and enhancing plant growth. Additionally, certain fungi are involved in bioremediation, helping to degrade pollutants in the environment.
As for human uses and diseases, fungi have significant implications. Some fungi are used in food production, such as yeast in baking and brewing. They also produce various antibiotics, enzymes, and other valuable compounds. However, certain fungi can cause diseases in humans, ranging from superficial infections to severe systemic illnesses, such as fungal pneumonia or systemic candidiasis.
To provide more specific information about the phyla, ecosystem services, or human uses and diseases of a particular fungus, please provide the name or description of the fungus you are referring to.
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According to the image which represents a chromosome, which two
genes are most likely to have the largest amount of crossing over
between them?
- e + f
- a + e
- b + c
- a + c
To determine which two genes are most likely to have the largest amount of crossing over between them, we need to look for regions on the chromosome where there are multiple crossovers. In the given options, the image representing a chromosome is not available for reference. However, I can provide you with some general information regarding crossing over and gene location.
Crossing over occurs during meiosis when homologous chromosomes exchange genetic material. It typically happens between two non-sister chromatids at points called chiasmata. The frequency of crossing over varies along the length of the chromosome.
The likelihood of crossing over between two genes depends on their physical distance from each other on the chromosome. Genes that are located farther apart are more likely to undergo crossing over than genes that are closely linked.
Without the specific image or information about the physical distances between the genes in question, it is not possible to determine with certainty which two genes are most likely to have the largest amount of crossing over.
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discuss in a paragraph
organization of the nervous system in
humans, the reflex arc, the autonomic system
thank you
The nervous system is an intricate network of neurons that transmit information throughout the body and enable us to interact with the environment. It is divided into two primary divisions: the central nervous system (CNS) and the peripheral nervous system (PNS).
The CNS includes the brain and spinal cord, while the PNS includes all the other nerves in the body. The PNS is subdivided into two categories: the somatic nervous system (SNS) and the autonomic nervous system (ANS).
The SNS is responsible for voluntary movements and sensation, while the ANS regulates involuntary functions such as breathing, digestion, and heart rate.
The ANS has two subdivisions: the sympathetic nervous system (SNS) and the parasympathetic nervous system (PNS). The SNS prepares the body for physical activity, while the PNS is responsible for rest and digestion.
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Vince and Sandra both don't have down syndrome. They have two kids. with down Syndrome. vince brother has down syndrome and his sister has two kids. with down Syndrome. which statement is Correct ..... a. Vince has 45 chromosomes b. Vince brother has 45 chromosomes. c. Vince sister has 47 chromosomes. d. Vince sister has 46 chromose e. Vince and sandra kids have 47 chromosomes
The correct statement is that Vince's sister, like Vince and Sandra, has the usual 46 chromosomes.
Based on the information provided, the correct statement is d. Vince's sister has 46 chromosomes. Down syndrome is a chromosomal disorder caused by the presence of an extra copy of chromosome 21, resulting in a total of 47 chromosomes instead of the usual 46. It is typically caused by a nondisjunction event during cell division, where an extra copy of chromosome 21 is present in the sperm or egg that contributes to the formation of the embryo. In the given scenario, both Vince and Sandra do not have Down syndrome, which means they have the normal chromosomal complement of 46 chromosomes. However, they have two children with Down syndrome. This suggests that one or both of them may carry a translocation or other genetic abnormality that increases the risk of having a child with Down syndrome. Vince's brother having Down syndrome does not provide any information about Vince's chromosome count, as Down syndrome can occur sporadically in individuals with no family history of the condition.
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A mutant sex-linked trait called "notched" (N) is deadly in Drosophila when homozygous in females. Males who have a single N allele will also die. The heterozygous condition (Nn) causes small notches on the wing. The normal condition in both male and females is represented by the allele n. Which of the following statement is incorrect about the F1 generation from the cross between XNXn and XnY?
a. Among the male flies, 50% have normal wings and 50% have small notches on the wings. b. The ratio of the male flies and the female flies is 1:2.
c. All the male flies have normal wings.
d. Among the female flies, 50% have normal wings and 50% have small notches on the wings. e. Pleiotropy may be used to describe this gene.
The statement that is incorrect about the F1 generation from the cross between XNXn and XnY is option c. All the male flies have normal wings.
In Drosophila, the "notched" (N) trait is lethal when homozygous in females and also lethal in males with a single N allele. The heterozygous condition (Nn) causes small notches on the wing. In the given cross between XNXn (female) and XnY (male), the genotype of the offspring can be represented as follows:
Male flies: 50% will have normal wings (XnY) and 50% will have small notches on the wings (XNXn).
Female flies: 50% will have normal wings (XnXn) and 50% will have small notches on the wings (XNXn).
Therefore, the correct statement is that among the male flies, 50% have normal wings and 50% have small notches on the wings. The ratio of male flies to female flies is 1:1, not 1:2 as mentioned in option b. Additionally, it is incorrect to say that all male flies have normal wings, as some will have small notches due to the presence of the N allele. Pleiotropy, the phenomenon where a single gene affects multiple traits, may be applicable to describe the "notched" gene since it influences wing morphology and viability in both sexes.
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Question 30 30 Pyrogens are: 1. fever-inducing substances. 2. phagocytosis-enhancing substances 3. complement activators 4. fever-inhibiting substances 3 O O t 02 01 Previous 1 pts
Pyrogens are fever-inducing substances (Option 1). Pyrogens are a type of substance that causes fever in the body. Pyrogens can come from different sources, including bacteria, viruses, and chemicals.
Pyrogens are detected by the body's immune system, which then sends signals to the brain to increase the body's temperature to combat the infection. This is why fever is often a sign of infection or illness. Pyrogens can be produced by the body as well as by external sources such as infectious agents and synthetic materials. The pyrogen produced by the body is known as endogenous pyrogen.
They are primarily produced by mononuclear cells and phagocytes in response to infection, inflammation, or trauma. Pyrogens produced by exogenous sources, such as infectious agents, are known as exogenous pyrogens. These pyrogens are produced by a variety of microorganisms and are released into the bloodstream as a result of infection. Hence, 1 is the correct option.
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Humans affect the carbon cycle by which of the following? destroying vegetation that absorbs carbon dioxide. clearing or cutting down forests. burning fossil fuels. All of the choices are correct.
All of the choices are correct. Humans affect the carbon cycle by destroying vegetation that absorbs carbon dioxide, clearing or cutting down forests, and burning fossil fuels.What is the Carbon Cycle?Carbon is a basic constituent of all life forms on Earth.
It is the foundation of all life and an essential component of all organic compounds. Carbon dioxide (CO2) is a greenhouse gas that contributes to global climate change when it is present in the atmosphere. However, the majority of the carbon on Earth is held in rocks and sediments.Carbon cycles between the atmosphere, oceans, land, and living things in a number of different ways. The carbon cycle is the process by which carbon is passed through living and non-living things, and it is crucial to life on Earth.
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3. How many green?. 3 How many albino? 4. What is the ratio of green to albino?3/1 Reduce your ratio by dividing green by albino, and round to one decimal place. 3.0 5. How closely does the observed corn seedlings ratio agree with the expected phenotypic ratio calculated previously? 6. What will happen to all the albino seedlings? Explain. 7. Since the albinos die before they can reproduce, how does the trait of albinism continue in some plant populations?
In the given scenario, there are 3 green seedlings and the ratio of green to albino seedlings is 3:1. The observed ratio closely matches the expected phenotypic ratio.
3. As for the albino seedlings, they are likely to die as they lack the necessary pigments for survival. However, the trait of albinism can continue in plant populations through various mechanisms such as sporadic mutations or genetic recombination.
4. According to the given information, there are 3 green seedlings and the ratio of green to albino seedlings is 3:1. This means that for every 3 green seedlings, there is 1 albino seedling. By dividing the number of green seedlings (3) by the number of albino seedlings (1), we get a ratio of 3.0
5. The observed ratio of green to albino seedlings closely matches the expected phenotypic ratio of 3:1. This suggests that the inheritance of the trait follows Mendelian principles, where the green phenotype is dominant and the albino phenotype is recessive.
6. As for the albino seedlings, they are likely to die before reaching maturity. Albinism is characterized by the absence of pigments, including chlorophyll, which is essential for photosynthesis and plant survival. Without chlorophyll, albino seedlings cannot produce energy from sunlight and are unable to carry out vital metabolic processes.
7. However, the trait of albinism can still continue in plant populations through various mechanisms. Sporadic mutations can introduce new albino individuals, and if these individuals manage to reproduce selective breeding before dying, they can pass on the albino trait to their offspring.
Additionally, genetic recombination during sexual reproduction can shuffle and recombine genes, potentially producing albino offspring even in populations where the trait is rare. These mechanisms contribute to the persistence of the albinism trait in some plant populations, despite the lower fitness and survival of albino individuals.
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How is the structure of the lamprey's gills adapted to their function? Give at least 3 exemples, please.
Lampreys are a group of jawless fish that lack paired appendages and a true backbone. Their gills are specialized structures that are adapted to their aquatic lifestyle.
Here are three examples of how the structure of lamprey gills is adapted to their function:1. Filamentous structure: The filamentous structure of the gill filaments increases the surface area available for gas exchange. This allows for efficient uptake of oxygen and removal of carbon dioxide. The filaments also contain blood vessels that transport oxygen to the rest of the body.
Countercurrent exchange: The countercurrent exchange mechanism in lamprey gills maximizes the uptake of oxygen from the water. Blood flows in the opposite direction to the flow of water over the gill filaments. This creates a concentration gradient that allows for efficient oxygen uptake.3. Mucous secretion: Lamprey gills secrete a layer of mucus that helps to trap particles in the water, such as bacteria and algae.
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The Ames Test uses a Salmonella enterica mutant strain that is unable to grow in the absence of histidine. How is the mutant strain used to test whether a compound is mutagenic? O A. The strain is used to measure rat liver enzymatic activity. O B. The strain is used to estimate how many forward mutations a tested compound causes that lead to the mutant phenotype. O C. The strain is used to determine how many more back mutations a tested compound causes that restore wild-type growth. D. The strain is used produce the histidine needed for the test. O E. The strain is used for DNA sequencing to determine the number of mutations caused by a tested compound.
The Ames Test uses a Salmonella enterica mutant strain that is unable to grow in the absence of histidine. How the mutant strain used to test whether a compound is mutagenic is that it is used to estimate how many forward mutations a tested compound causes that lead to the mutant phenotype.Option B is the correct option.
The Ames Test is used to test whether chemicals are mutagenic. Mutagenic chemicals are those that cause mutations in the DNA of an organism.The test makes use of a strain of Salmonella bacteria that is unable to grow in the absence of histidine. The bacteria are treated with a chemical to be tested for mutagenicity, as well as a small amount of histidine to enable the bacteria to grow if mutations revert the bacteria back to the wild type.
These bacteria are plated on a medium that lacks histidine, and the number of revertant colonies is counted after a 24- to 48-hour incubation period.The number of revertant colonies is then compared to the number of colonies that grew in a control experiment that did not contain the test compound. The more colonies that revert to a wild-type phenotype in the presence of the test compound, the more mutagenic it is assumed to be. The assay is useful because it is both quick and relatively inexpensive, and it is capable of detecting a wide range of different types of mutagens.
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0. Sodium pyrophosphate can effect what in a muscle? (2 points) 1. How can I use UV and Commassie blue staining to detect proteins in the lab you experienced i.e. what does commassie blue stain and wh
Coomassie Brilliant Blue is generally used for the discovery of proteins in sodium dodecyl sulfate- polyacrylamide gel electrophoresis, owing to its trustability and simplicity.
Then, we report dramatically dropped protein staining and destaining time, as well as significantly increased discovery perceptivity with the operation of enhanced heat. The staining time was 5 min at 55,62.5, or 70 °C for a1.5- mm gel, while it took 45, 45, and 20 min, independently, for destaining. The staining time could be reduced to 1 min for a0.8 mm gel stained at 65 °C, to 2 min at 60 °C and 5 min at 55 °C. The destaining of proteins anatomized on a0.8 mm gel could be fulfilled in 8, 15, and 20 min at 65, 60, and 55 °C, independently. operation of heat, therefore, enables proteins to be stained and destained fleetly, as well as enhancing discovery perceptivity.
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Mature T cells express either the co-receptor CD4 or CD8. Give
two (2) reasons why the expression of a co-receptor is important
for the activation and function of T cells.
Mature T cells express either the co-receptor CD4 or CD8. The expression of a co-receptor is important for the activation and function of T cells.
The following are two reasons why the expression of a co-receptor is important for the activation and function of T cells:
1. Enhances the specificity of T cellsCD4 and CD8 are critical for T cell development and function, and they aid in antigen recognition. CD4 is important for activating MHC class II-restricted T helper cells, whereas CD8 is important for activating MHC class I-restricted cytotoxic T cells.
The expression of these co-receptors aids in the recognition of the major histocompatibility complex (MHC) molecules, which improves the specificity of T cell responses.
2. Co-receptors provide additional signaling
The expression of CD4 or CD8 on T cells aids in the recognition of peptides bound to MHC molecules. In addition, these molecules provide co-stimulatory signals to T cells, which are essential for full T cell activation.
Co-receptors aid in T cell activation by providing additional signaling to T cells to elicit an effective immune response.
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Compare the similarities and differences of the forelimbs and
hindlimbs of shark, milkfish, frog, turtle, chicken and cat.
The forelimbs and hindlimbs of sharks, milkfish, frogs, turtles, chickens, and cats exhibit both similarities and differences in their structure and function.
While the specific anatomical details may vary among these animals, there are some commonalities and distinctions in the forelimbs and hindlimbs. In general, these limbs are adapted for locomotion and may have similar bone structures, including humerus, radius, and ulna in the forelimbs, and femur, tibia, and fibula in the hindlimbs. However, the proportions, sizes, and mobility of these bones can differ based on the animal's habitat and mode of locomotion. For instance, sharks have pectoral fins as their forelimbs, which are adapted for swimming, while cats have highly flexible and retractable claws for capturing prey. Frogs and turtles have webbed feet for swimming, whereas chickens have modified forelimbs as wings for flight. These variations reflect the diverse adaptations of these animals to their respective environments and lifestyles.
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In biology,
dehydration synthesis (building) of molecules results in what we
call
Group of answer choices
a. Organics
b. Chemicals
c. Nucleic acids
d. Macromolecules
Dehydration synthesis refers to the process of building macromolecules by removing water molecules. It results in the formation of larger molecules from smaller subunits.
The correct option is d. Macromolecules
Dehydration synthesis, also known as condensation reaction, is a chemical process that occurs in biology to build macromolecules. During dehydration synthesis, smaller subunits are joined together by removing a water molecule. This process is essential for the formation of various macromolecules, including carbohydrates, proteins, and nucleic acids.
For example, in the case of carbohydrates, monosaccharides (simple sugars) can undergo dehydration synthesis to form disaccharides or polysaccharides. In this process, the hydroxyl (-OH) group from one monosaccharide and a hydrogen atom from another monosaccharide combine to form water, while the remaining oxygen and carbon atoms bond together to create a glycosidic linkage, connecting the two sugar molecules.
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1. The protocol used by Harju et al. (2004) extracts total nucleic acids, i.e. DNA and RNA. In most cases we also need to do an additional step to ensure that we only end up with pure DNA. Give
one way in which we can eliminate RNA from a DNA sample.
2. What does chloroform do in nucleic acid extraction?
3. Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and 70% ethanol, in succession. What happens during these steps and why are they essential?
4. Spectrophotometric detection of nucleic acids require readings at wavelengths of 260nm, and 280nm. What is the significance of these wavelengths?
5. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?
6. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?
To eliminate RNA from a DNA sample, we can use RNase A or RNase T1 enzymes, which will degrade RNA into small oligonucleotides, which can be further eliminated by precipitation or chromatography.
1. To eliminate RNA from a DNA sample, we can use RNase A or RNase T1 enzymes, which will degrade RNA into small oligonucleotides, which can be further eliminated by precipitation or chromatography.2. In nucleic acid extraction, chloroform is used as an organic solvent to dissolve lipids and remove proteins from the sample.3. The use of two kinds of ethanol, 100% and 70%, helps to precipitate the DNA in the sample. The 100% ethanol helps in the initial precipitation, while the 70% ethanol is used to wash the DNA pellet to remove any impurities.4. The significance of wavelengths 260nm and 280nm in spectrophotometric detection of nucleic acids is that DNA and RNA absorb light at these wavelengths.5.
A pure DNA sample will have an A260/280 ratio of around 1.8, while a pure RNA sample will have a ratio of around 2.0. A ratio of 1.5 indicates the presence of protein contamination.6. Fluorometry detects DNA by using fluorescent dyes that bind specifically to DNA molecules, and this technique is more sensitive than spectrophotometry because it can detect small amounts of DNA even in the presence of other contaminants.
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*A detailed explanation of why*
homologous recombination of DNA can happen during G2 phase of mitosis (after DNA synthesis) or during M-phase of meiosis (when chromosomes are paired). In both cases many of the mechanisms are the same. In G2 phase, the purpose is to repair breaks in the DNA whereas in meiosis, it is about sticking homologous chromosomes together. For homologous recombination
During G2 phase of mitosis or during M-phase of meiosis, homologous recombination of DNA is necessary to repair DNA damage and preserve genomic integrity.
Homologous recombination of DNA can occur during G2 phase of mitosis (after DNA synthesis) or during M-phase of meiosis (when chromosomes are paired) due to many of the mechanisms that are the same in both cases.
In G2 phase, the purpose is to repair breaks in the DNA whereas in meiosis, it is about sticking homologous chromosomes together. Homologous recombination of DNA has a key role in repair and the preservation of genomic integrity by allowing the repair of DNA double-strand breaks (DSBs).
DNA repair is necessary due to DNA damage caused by exposure to environmental agents or endogenous agents like free radicals.
When there is a DSB in DNA, the ends of the break are resected by exonucleases, and the resulting single-stranded DNA (ssDNA) is coated with replication protein A (RPA). RPA is then replaced by a RAD51 recombinase filament, which initiates homologous recombination. During homologous recombination, the ss
DNA searches for a homologous region of the genome, which it then uses as a template for repair. This homologous template can be found on a sister chromatid or on the homologous chromosome. After the ssDNA invades the homologous region of DNA, DNA synthesis occurs, and the DSB is repaired.
Therefore, during G2 phase of mitosis or during M-phase of meiosis, homologous recombination of DNA is necessary to repair DNA damage and preserve genomic integrity.
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Which population group in New Zealand has the highest prevalence of chronic hepatitis B virus infection?
Chinese females aged 0-10 years
European males aged 20-30 years
Maori males aged 10-20 years
Pacific islands female aged 30-40 years
Among the given population group in New Zealand, Pacific Islands female aged 30-40 years have the highest prevalence of chronic hepatitis B virus infection.
What is chronic hepatitis B virus infection?
Chronic hepatitis B virus infection is a condition when a person's immune system does not successfully remove the hepatitis B virus from their liver after six months or more. A person who has chronic hepatitis B virus infection can develop liver damage such as liver scarring (cirrhosis), liver cancer or even liver failure.Chronic hepatitis B virus infection is endemic in the Pacific region, and the Pacific Islander community residing in New Zealand are disproportionately affected by this virus than any other population group.
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Energetics [20] a) Graphically illustrate the influence of body mass on total metabolic rate of mammals (graph axes should be appropriately labelled). State the exponential equation that describes the relationship you have drawn? Explain the use of allometric scaling relationships and how can they be used to infer adaptation? [8] + b) Discuss the selective pressurer (climato ar
Similarly, organisms that live in hot, arid regions are adapted to conserve water, such as the kangaroo rat, which can survive without drinking water. Therefore, selective pressure due to climatic conditions has played a significant role in shaping the adaptations of organisms to their environments.
a) Influence of body mass on total metabolic rate of mammals:The influence of body mass on total metabolic rate of mammals can be shown in the graph below. The Y-axis represents metabolic rate in Watts and the X-axis represents the mass of the animal in kg. According to the graph, the metabolic rate increases as the mass of the animal increases.Graph:Allometric Scaling Relationships:Allometric scaling is the study of the relationship between body size and physiological variables. According to the allometric scaling relationship, physiological variables increase or decrease as a power of body size.The exponential equation that describes the relationship between body mass and metabolic rate in mammals is given as y
= aMb, where "y" is the metabolic rate, "a" is the constant of proportionality, "M" is the body mass of the mammal, and "b" is the scaling exponent or slope of the line. This equation is referred to as the allometric equation.Use of Allometric Scaling Relationships to Infer Adaptation:Allometric scaling relationships can be used to infer adaptation in organisms by identifying differences in scaling exponents among groups of organisms. In other words, the scaling exponents reveal how physiological variables change with body mass across different groups of organisms. These differences can provide insights into how organisms are adapted to different environments and lifestyles. For example, animals that have a higher metabolic rate than expected for their body size might be adapted to high-energy environments such as tropical rainforests. On the other hand, animals that have a lower metabolic rate than expected for their body size might be adapted to low-energy environments such as polar regions.b) Selective Pressure (Climatic Conditions):Climatic conditions exert selective pressure on organisms, which can lead to adaptations to the prevailing environmental conditions. For example, organisms that live in polar regions are exposed to low temperatures and scarce food resources, which has resulted in adaptations such as thick fur, blubber, and reduced metabolic rates. Similarly, organisms that live in hot, arid regions are adapted to conserve water, such as the kangaroo rat, which can survive without drinking water. Therefore, selective pressure due to climatic conditions has played a significant role in shaping the adaptations of organisms to their environments.
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Asthma may lead to (more than one answer may apply) a.partial obstructions of the small bronchi and bronchioles with air trapping.
b. total obstruction of the airway leading to atelectasis.
c. acidosis. d.hypoxemia.
Asthma may lead to the following:
a. Partial obstructions of the small bronchi and bronchioles with air trapping: Asthma is characterized by inflammation and constriction of the airways, which can cause narrowing and obstruction of the bronchi and bronchioles. This can result in difficulty exhaling fully and air getting trapped in the lungs.
d. Hypoxemia: Asthma attacks can cause a decrease in the amount of oxygen in the blood, leading to hypoxemia. This occurs due to the impaired exchange of oxygen and carbon dioxide in the constricted airways.
It is important to note that asthma does not typically cause total obstruction of the airway leading to atelectasis (b) or acidosis (c). However, severe asthma attacks can potentially lead to complications such as respiratory failure, which could result in atelectasis or acidosis.
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Order the steps of protein synthesis into the RER lumen.
ER signal sequences binds to signal recognition particle The signal recognition particle receptor binds the signal recognition particle - ER signal sequence complex translocon closes
ER signal is cut off, ribosome continues protein synthesis The newly formed GTPase hydrolyses GTP, translocon opens protein passes partially through the ER lumen ribosome detaches, protein passes completely into ER lumen Ribosome synthesizes ER signal sequenc
Protein synthesis in RER lumen involves several steps, which occur in a sequential order.
The correct sequence of steps involved in protein synthesis into the RER lumen is as follows:
1. Ribosome synthesizes ER signal sequence.
2. ER signal sequences bind to signal recognition particle.
3. The signal recognition particle-receptor binds the signal recognition particle-ER signal sequence complex.
4. Translocon closes.
5. Ribosome continues protein synthesis.
6. The newly formed GTPase hydrolyzes GTP, and the translocon opens.
7. Protein passes partially through the ER lumen.
8. ER signal is cut off.
9. Ribosome detaches, and protein passes completely into the ER lumen.
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How many different tRNAs are used in translation? What is a "charged" tRNA? How does a tRNA "know where to place its amino acid cargo? What process is used to accomplish DNA replication, transcription, and translation? How does the ribosome organize the incoming RNAs to add amino acids in the correct order? What is the purpose of each of the A. P, and E sites on the ribosome? Where (at what codon) does translation begin? How does the RNA in the ribosome's "A" site get to the "psite? What is the purpose of a signal sequence" on a newly made polypeptide? How is a nibosome that is bound to the rough endoplasmic reticulum different from a ribosome that is free in the cytoplasın? How is the translation machinery that translates messages encoded by the mitochondrial and plastid DNAs different from the machinery that translates nuclear messages? How are polypeptides modified after translation to make them ready to function normally?
Ribosomes arrange their assembly within the correct order during translation, and tRNAs transport specific amino acids.
How are polypeptides modified after translation to make them ready to function normally?1. In Translation, there are twenty particular sorts of tRNA, each of which is related to a specific amino acid
2. A tRNA particle that's bound to its comparing amino destructive is known as a "charged" tRNA.
3. In the midst of elucidation, the range of the amino destructive cargo is chosen by mixing the anticodon on the tRNA iota with the codon on the mRNA.
4. The shapes of DNA replication, interpretation, and elucidation, independently, are what makes DNA replication, interpretation, and translation conceivable.s.
5. In arrange to guarantee that amino acids are included within the redress arranged amid interpretation, the ribosome orchestrates the approaching mRNA and tRNA particles in its A, P, and E destinations.
6. The aminoacyl-tRNA that comes in is put away at the A location, the peptidyl-tRNA is put away at the P location, and the deacylated tRNA exits at the E location.
7. The beginning b, AUG, is typically where translation starts.
8. Translocation is the method by which the tRNA within the ribosome's A location moves to the P location.
9. A recently synthesized polypeptide is coordinated to the fitting cellular compartment or organelle by a flag grouping.
10. Free ribosomes create proteins for the cytoplasm, while ribosomes bound to the unpleasant endoplasmic reticulum create proteins for emission or film addition.
11. In terms of ribosomal components and tRNA sets, the mitochondrial and plastid DNA interpretation apparatus is particular from the atomic interpretation apparatus.
12. Polypeptides go through distinctive alterations after translation, counting collapsing, post-translational changes (e.g., phosphorylation, glycosylation), and centering to express cell compartments or organelles to engage their fitting capability.
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Thank you for a great sem 2 pts Question 22 The normal number of platelets found in blood is: O 130,000 to 400.000/ul O 75,000 to 525,000/ul O 100.000 to 500.000/ul O 300,000 to 650,000/ul O 25.000 to
Option a is correct. The normal range of platelet count in the blood is typically between 130,000 and 400,000 per microliter.
Platelets are tiny blood cells that play a crucial role in blood clotting and preventing excessive bleeding. The normal range of platelet count in the blood is an important indicator of overall health. A platelet count below 130,000 per microliter is considered low and may indicate a condition known as thrombocytopenia, which can lead to increased risk of bleeding.
On the other hand, a platelet count above 400,000 per microliter is considered high and may be indicative of a condition called thrombocytosis, which can increase the risk of blood clots. It's important to note that the normal range may vary slightly depending on the laboratory conducting the analysis. If a platelet count falls outside the normal range, further medical evaluation may be necessary to determine the underlying cause.
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A full step by step example of Translation:
Here is an mRNA sequence:
mRNA 5’ --GACCTTAUAUUUUGACUGCA AUGAGUCCUGAUGUUUGAGGACU --3’
How do you ‘read’ it?
First, look for the promoter region (a TATAAA box, but in RNA language)
mRNA 5’ --GACCTTAUAUUUUGACUGCAAUG AGACCUGAUGUUUGAGGACU--3’
Then find the first start codon after the promoter
mRNA 5’ --GACCTTAUAUUUUGACUGCAAUG AGACCUGAUGUUUGAGGACU--3’
Then start coding in triplets, continue until you reach a stop triplet
mRNA 5’ --GACCTTAUAUUUUGACUGCAAUG AGA CCU GAU GUU UGA GGACU--3’
amino acid: start- arginine- proline- aspartic-valine-stop
ASSIGNMENT
For the DNA sequence given below, write the complementary DNA sequence that would complete the double-strand.
DNA
3’-
T
G
C
T
T
A
C
G
T
A
T
- 5’
DNA
5’-
Does it matter which strand is the ‘code strand’? The following two sequences look identical, except one runs 3’-5’ and the other 5’-3’. For each DNA sequence given below, write the mRNA sequence that would be coded from it. Make sure you indicate the direction of each mRNA strand (i.e. 3’ and 5’ ends). Use the Universal triplet code to determine the sequence of amino acids that would be generated for each of the mRNA sequences that you generated in question 2. Remember that the reading of mRNA goes in the 5’-3’ direction (see lab notes for examples). WHY is there a reading direction? The enzymes involved have got "handedness" or directional shapes to them, and only work in one direction.
The complementary DNA sequence to the given DNA strand is written in the 5'-3' direction. The reading direction of mRNA is from the 5'-3' end, which is necessary for the enzymes involved in transcription and translation to properly read and synthesize the mRNA sequence.
To complete the double-strand DNA sequence, we need to find the complementary bases for each base in the given sequence. The complementary bases are as follows:
DNA
3’- A C G A A T G C A T -5’
DNA
5’- T G C T T A C G T A -3’
For the mRNA sequence, we need to replace thymine (T) with uracil (U) since mRNA contains uracil instead of thymine. The mRNA sequence would be:
mRNA
5’- A C G A A U G C A U -3’
The reading direction of mRNA is from the 5' end to the 3' end because the enzymes involved in transcription and translation have a directional shape and can only work in one direction. This ensures the accurate reading and synthesis of the mRNA and subsequent protein production.
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Question 4: a. Describe an experiment by means of which you can demonstrate that after treatment of human oviduct cells with estrogen, a full-length copy of the ovalbumin mRNA is synthesized (2155 bp linear mRNA). [3] b. There are two versions of the thyroid hormone receptor produced in human cells. These two proteins differ in size and are produced in different relative amounts in tissue A and tissue B. How would you experimentally demonstrate that the difference between A and B is determined by alternative splicing? C. You would like to study the different proteins that are synthesized after induction with a hormone. a. Describe the type of information you can obtain from 2D electrophoresis. [3] How can you use the protein spots, unique to cells stimulated with hormone, to obtain information of their identity? [1]
In order to identify the proteins that are unique to cells stimulated with hormone, we can excise the protein spot from the 2D gel and subject it to mass spectrometry. Mass spectrometry can be used to determine the identity of the protein based on its peptide sequence.
a. In order to demonstrate that after treatment of human oviduct cells with estrogen, a full-length copy of the ovalbumin mRNA is synthesized (2155 bp linear mRNA), we can perform a Northern blot analysis or reverse transcription polymerase chain reaction (RT-PCR).Northern blot analysis is a technique that is used to detect and quantify mRNA. RNA is first separated by gel electrophoresis based on size and then transferred to a nylon membrane. The membrane is then hybridized with a radiolabeled probe specific to the mRNA of interest. A full-length copy of the ovalbumin mRNA will be detected on the Northern blot if it is synthesized in response to estrogen treatment.RT-PCR is a technique that is used to amplify a specific RNA sequence. In this case, RNA is first reverse transcribed into cDNA and then amplified using PCR with primers specific to the ovalbumin mRNA. The amplified product will be the full-length copy of the ovalbumin mRNA if it is synthesized in response to estrogen treatment.
b. Alternative splicing is a process that allows the production of different protein isoforms from a single gene. In order to experimentally demonstrate that the difference between A and B is determined by alternative splicing, we can perform a reverse transcription polymerase chain reaction (RT-PCR) followed by gel electrophoresis. RT-PCR is a technique that is used to amplify a specific RNA sequence. In this case, RNA is first reverse transcribed into cDNA and then amplified using PCR with primers that flank the alternative splicing site. Gel electrophoresis is then used to separate the amplified products based on size. If the two versions of the thyroid hormone receptor are produced by alternative splicing, we would expect to see two different size bands on the gel, corresponding to the two different isoforms.
C. 2D electrophoresis is a technique that is used to separate proteins based on their isoelectric point (pI) and molecular weight. In the first dimension, proteins are separated by isoelectric focusing (IEF), which separates proteins based on their pI. In the second dimension, proteins are separated by SDS-PAGE, which separates proteins based on their molecular weight. The result is a 2D gel with protein spots that can be visualized with a stain such as Coomassie blue or silver stain.In order to identify the proteins that are unique to cells stimulated with hormone, we can excise the protein spot from the 2D gel and subject it to mass spectrometry. Mass spectrometry can be used to determine the identity of the protein based on its peptide sequence.
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In response to low blood pressure indicate if the following will increase or decrease (i.e., during the baroreceptor reflex to return BP to normal): 1. heart rate 2. stroke volume 3. blood vessel diameter 4. peripheral resistance HR SV Vessel diameter PR
The Baroreceptor Reflex responds to changes in blood pressure, by adjusting heart rate, peripheral resistance, and stroke volume. These adjustments keep the blood pressure within its normal range, and prevent it from falling or rising drastically.
When the blood pressure is low, the Baroreceptor Reflex kicks in and makes several adjustments to increase the blood pressure. These adjustments are made by adjusting the heart rate, stroke volume, blood vessel diameter, and peripheral resistance. These adjustments are as follows:1. Heart rate increases when blood pressure decreases.2. Stroke volume increases when blood pressure decreases.3.
Blood vessel diameter decreases when blood pressure decreases.4. Peripheral resistance increases when blood pressure decreases.
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- Briefly describe the disorder you chose and the part(s) of the skeletal system that it affects. - Outline the causes of the disorder (if known) and the symptoms that accompany it. - Describe the current treatments that are available and how they work.
A Brief Discussion of Marfan Syndrome Marfan syndrome is a rare, hereditary disorder that affects the skeletal and cardiovascular systems. Marfan syndrome affects about one in every 5,000 people, with men and women being equally affected. The disease is caused by mutations in the FBN1 gene, which encodes the protein fibrillin-1, which is a component of connective tissue.
Marfan syndrome causes a variety of skeletal and cardiovascular abnormalities, including scoliosis, chest wall deformities, tall stature, and aortic aneurysms, among other things. Marfan syndrome is caused by mutations in the FBN1 gene, which encodes the protein fibrillin-1, which is a component of connective tissue.Marfan syndrome is caused by mutations in the FBN1 gene, which encodes the protein fibrillin-1, which is a component of connective tissue.
Fibrillin-1 provides elasticity and strength to connective tissues, and mutations in this gene can cause abnormalities in connective tissue development. This can lead to weakened blood vessels and connective tissue throughout the body, including the skeleton. Current therapies for Marfan syndrome aim to alleviate symptoms and slow or prevent disease progression.
Treatment may include beta-blockers, which reduce the risk of aortic rupture or dissection, and/or angiotensin receptor blockers, which have been shown to slow aortic dilation. Surgery may be required to repair damaged blood vessels or correct skeletal deformities. Individuals with Marfan syndrome should receive ongoing monitoring and care from a medical professional with experience treating the disease.
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Question 4 0.5 pts Which of the following provides the force to push fluids within the glomerulus into the capsule? O Blood Pressure O Osmotic Potential O Skeletal Muscle Contractions O Gravity Questi
The blood pressure provides the force to push fluids within the glomerulus into the capsule.
The glomerulus is a tiny blood vessel inside the kidney that is involved in the blood filtration process. Its primary function is to filter blood from the renal arteriole (a blood vessel that enters the kidney) and eliminate waste from the bloodstream by allowing water and small molecules to pass through it. The fluid that passes through the glomerulus is referred to as the filtrate or ultrafiltrate.
The Bowman's capsule, also known as the renal corpuscular capsule, surrounds the glomerulus and is part of the kidney's filtration process. The glomerulus filters blood into the Bowman's capsule, which then transports it to the proximal convoluted tubule, where further filtration and processing occur. The Bowman's capsule is critical in preserving the kidneys' ability to filter waste and produce urine.
The force to push fluids within the glomerulus into the capsule is provided by blood pressure. Blood pressure, which is the pressure exerted by the blood on the walls of blood vessels, pushes blood through the kidney, allowing it to be filtered by the glomerulus. As a result, the glomerulus filters waste from the blood and passes it into the Bowman's capsule, which transports it to the proximal convoluted tubule for additional processing.
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What modern technology allowed the architect to study the pyramid more intensely? (1point)
Light Detection and Ranging (LiDAR) technology has enabled architects to conduct more in-depth studies of pyramids, providing precise 3D models and revealing hidden features. By utilizing laser beams to scan the structures, LiDAR captures detailed data on dimensions, angles, and construction techniques, offering insights into their design and potential undiscovered chambers.
One modern technology that allowed architects to study the pyramid more intensely is LiDAR (Light Detection and Ranging).
LiDAR is a remote sensing method that uses laser pulses to measure distances and create highly accurate three-dimensional models of objects and surfaces.
It has revolutionized the field of archaeology and architectural research by providing detailed and precise data about ancient structures like pyramids.
With the help of LiDAR technology, architects can conduct non-invasive surveys of pyramids, capturing precise measurements of their dimensions, contours, and intricate details.
LiDAR scanners mounted on aircraft or drones can quickly and efficiently collect massive amounts of data by emitting laser beams and measuring the time it takes for the light to return after bouncing off the pyramid's surface.
The resulting point cloud data can be processed and analyzed using advanced software, allowing architects to create comprehensive 3D models of the pyramid.
These models can be manipulated and studied from different angles, revealing hidden features, structural anomalies, and architectural insights that were previously difficult to ascertain.
LiDAR technology enables architects to delve deeper into the study of pyramids, aiding in the understanding of their construction techniques, historical significance, and cultural context.
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Put the following muscle contraction and relaxation steps in order: acetylcholine travels across the synaptic gap actin and myosin form linkages Camions diffuse into fiber; bind to troponin actin and myosin linkages are broken ACH released from distal end of motor neuron cholinesterase decomposes acetylcholine acetylcholine stimulates the skeletal fiber muscle relaxes calcium ions diffuse out of the skeletal muscle muscle fiber shortens (contracts)
The following muscle contraction and relaxation steps are in order: Acetylcholine travels across the synaptic gap, acetylcholine stimulates the skeletal fiber muscle, actin and myosin form linkages, calcium ions diffuse into the fiber; bind to troponin, actin and myosin linkages are broken, ACH released from the distal end of the motor neuron, cholinesterase decomposes acetylcholine, calcium ions diffuse out of the skeletal muscle, and muscle fiber shortens (contracts)
There are several steps in the process of muscle contraction and relaxation. They include acetylcholine, actin and myosin, cholinesterase, calcium ions, and more.
Here's the order in which they occur:
1. Acetylcholine travels across the synaptic gap: The first step is the release of acetylcholine from the motor neuron into the synaptic cleft. This neurotransmitter is then picked up by the muscle fiber.
2. Acetylcholine stimulates the skeletal fiber muscle: The acetylcholine then binds to receptors on the muscle fiber, causing the muscle to depolarize.
3. Actin and myosin form linkages: Once depolarization occurs, actin and myosin can form linkages, which cause the muscle to contract.
4. Calcium ions diffuse into the fiber; bind to troponin: Calcium ions then diffuse into the muscle fiber and bind to troponin, which is a protein in the muscle. This causes the muscle to contract even more.
5. Actin and myosin linkages are broken: Eventually, the actin and myosin linkages are broken, which allows the muscle to relax.
6. ACH released from the distal end of the motor neuron: Once the muscle has relaxed, the acetylcholine is released from the motor neuron again, and the cycle begins again.
7. Cholinesterase decomposes acetylcholine: Cholinesterase is an enzyme that breaks down acetylcholine, which stops the muscle from contracting.
8. Calcium ions diffuse out of the skeletal muscle: Calcium ions then diffuse out of the muscle fiber, which allows the muscle to relax even more.
9. Muscle fiber shortens (contracts): Finally, the muscle fiber shortens, causing the muscle to contract even more. This process continues until the muscle has reached its full contraction.
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Which of the following statements is most likely true about a cancer cell (when compared to its normal cell counterpart)? Select one OAA cancer cell undergoes higher levels of angiogenesis and is more likely to undergo apoptosis compared to its normal cell counterpart OB. A cancer cell has a low level of p53 activity and does not exhibit anchorage dependence compared to its normal cell counterpart OCA cancer cell has high level of p53 activity and exhibits density-dependent inhibition compared to its normal cell counterpart D.A cancer cell undergoes low levels of angiogenesis and is more likely to not undergo apoptosis compared to its normal cell counterpart
The most likely true statement about a cancer cell when compared to its normal cell counterpart is that a cancer cell has a low level of p53 activity and does not exhibit anchorage dependence compared to its normal cell counterpart (option B).
The p53 protein plays a critical role in regulating cell division and preventing the growth of abnormal cells. In cancer cells, mutations in the p53 gene can lead to reduced p53 activity, which compromises its ability to control cell growth and suppress tumor formation.
Anchorage dependence refers to the requirement of normal cells to be attached to a solid surface or extracellular matrix in order to divide and grow. Cancer cells, on the other hand, can exhibit anchorage independence, meaning they can grow and divide even in the absence of a solid surface or anchorage.
Therefore, option B best describes the characteristics often observed in cancer cells compared to their normal cell counterparts.
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