When you use the same histone protein as your standard, you determine the histone protein concentration to be 5 mg/ml. Explain the discrepancy (pick ONE answer): (5 points) a. The histone protein has fewer basic amino acids than albumin. b. The histone protein has more acidic amino acids than albumin c. The histone protein has more basic amino acids than albumin d. The histone protein contains more methionine than albumin

Answers

Answer 1

Answer:

A. The histone protein has fewer basic amino acids than albumin

Note: The question is incomplete. The complete question is given below:

You use the Bradford (coomassie) assay to determine the concentration of a whey protein. You use bovine albumin as your standard. You determine the histone protein concentration to be 3 mg/ml. When you use the same whey protein as your standard, you determine the histone protein concentration to be 5 mg/ml. Explain the discrepancy (pick ONE answer): (2 points) The histone protein has fewer basic amino acids than albumin. The histone protein has more acidic amino acids than albumin c. The histone protein has more basic amino acids than albumin d. The histone protein contains more methionine than albumin

Explanation:

The Bradford assay measures the concentration of proteins in a a protein sample using a color change that occurs from brown to blue when the protein in the sample binds to Coomassie dye. The Coomassie dye specifically binds to basic and aromatic amino acids in the protein. A standard curve is created using absorbance data from samples of proteins of known concentrations. The spectrophotometer is set to read at 595 nm in the Bradford assay as the peak absorbance of Coomassie Blue once bound to proteins occurs at 595 nm.

The higher the concentration of proteins, the higher the color intensity and the higher the absorbance values.

Since when bovine serum albumin was used as standard, the sample protein concentration was lesser than when the protein sample was used as standard, this means that the sample protein binds less to Coomassie dye as a standard thereby giving a higher sample protein concentration than the BSA standard. Therefore, the histone protein has fewer basic amino acids than albumin.


Related Questions

Please help I need it ASAP!!

Answers

The answer is T: cells, tissue, organs, organ system
T : cells, tissue, organ and organ systems

In humans, mitosis produces cells with ____ chromosomes and _____ chromosomes are found in the gametes after Meiosis.

Answers

Answer:

46, 23

Explanation:

Badgers are a type of mammal that eats mostly earthworms and insects. They sometimes eat small mammals, birds, and reptiles, as well as plant roots and fruit.

What kind of teeth would you expect a badger to have?
some flat and some round
all flat
some sharp and some flat
all sharp

Answers

Answer:

I wouldn't think badgers teeth are all that sharp so my answer is some sharp and some flat.

Answer:

its C

Explanation:

u can look up a picture of their teeth

A mutation is a (chance) of genetic material.

Many mutations are (neutral) (meaning not helpful nor harmful)


mutations Insertions and deletions are (?)​

Answers

Answer:

An insertion changes the number of DNA bases in a gene by adding a piece of DNA. As a result, the protein made by the gene may not function properly. Deletion. ... This type of mutation occurs when the addition or loss of DNA bases changes a gene's reading frame.

I need help. Thanks!

Answers

Answer:

They bounce around and take form of the container

Explanation:

what is the value of 6a + 3

Answers

Answer:

3 ( 2 + 1 )

Explanation:

Plant cells usually can be distinguished from animal cells because only plant cells have

Answers

Answer:

A plant cell contains a large, singular vacuole that is used for storage and maintaining the shape of the cell. In contrast, animal cells have many, smaller vacuoles. Plant cells have a cell wall, as well as a cell membrane. In plants, the cell wall surrounds the cell membrane.

Explanation:

Brainliest please?

The modern industrial agricultural practice that has resulted in high yields but with a loss in biodiversity is:

Answers

Answer: Monoculture

Explanation:

One type of crop at a time in one specific field. "Mono" means one.

What is a coral reef made of?
A. Grass
B. Dead animals
C. Volcanic rocks
D. Sand

Answers

The answer is B. Dead animals

Answer: B. Dead animals

Explanation:

Coral reefs are made with calcium. How do they get this calcium? From the skeletons of various organisms in the ocean.

AUG is generally a start codon. what is the amino acid that AUG codes for ?

Answers

AUG codes for methionine.

You are looking at the side of a cliff with many horizontal rock layers. In the upper layers you find fossils of rodents, but in layers further below you find amphibians but no rodents. In some still-deeper layers there are neither amphibians nor rodents but there are fossils of beetles and other insects. In the deepest layers you find no insects but you do find millipedes. This fossil evidence suggests that

Answers

Answer:

millipedes were the first of these animal groups to evolve.

Explanation:

Fossils are structural remains of animals from the past. As these animals die, it takes years for the remains to be buried.

The millipedes are at the lower layer of the rock, next to insects, amphibians, and then rodents. This shows that millipedes existed and evolved before the rest of the found animal fossils.

what is meant by the term VO2 max

Answers

Answer:

VO2 max is the maximum volume of oxygen you can consume per minute per kilogram of body weight at your maximum performance. VO2 max is an indication of aerobic fitness and should increase as your level of fitness improves.

Answer:

VO2 is the maximum oxygen uptake.

Explanation:

VO2 is the maximum oxygen consumption during incremental exercise.

Compact bone contains cylinders of calcified bone called ______________________. These cylinders consist of 4 to 20 Compact bone contains cylinders of calcified bone called concentric rings of bone known as:___________________.
a. osteons, canaliculi
b. lamellae, canaliculi
c. osteons, lamellae
d. Haversian canals, lamellae
e. none of the above

Answers

Answer:

The correct answer is c. osteons, lamellae

Explanation:

An osteon is the basic functional unit of the cortical bone of a tubular bone. An osteon consists of a central canal that contains two vessels of Havers. Concentric bony laminae are arranged around this canal. These lamellae consist of bone cells and the surrounding base substance. Between the Havers canals there are Volkmann canals that connect the Havers canals. The construction of the cortical bone in these functional units has not yet been fully clarified. In general, it is assumed that these structures serve primarily to deliver tissue and signal transduction. This structure is also supposed to serve the stability and adaptability of the bone.

When bonds are broken between atoms in molecules, energy is
a. stored as ADP
b. released
c. created
d. conserved

Answers

ANSWER: b. released

Explanation:

What two factors play a role in the type of snow flake created?

Answers

Answer:

Two factors play key roles in determining the shape of a snowflake: temperature and humidity.

Explanation:

Is Octane a mIxture??

Answers

Answer:

No

Explanation:

Octane is a straight-chain alkane composed of 8 carbon atoms. Octane is a colorless liquid with an odor of gasoline. Less dense than water and insoluble in water.

Answer:

Octane is a straight chain alkane made up of eight carbon atoms. ... N-octane is a colorless liquid with a diesel scent. Less thick than water, insoluble in water.

Explanation:

Description: Octane is a chain alkane.

Molecular formulas: C8H18 or CH3-(CH2)6-CH3

4. Which is produced in cellular respiration? A. Carbon dioxide B. Oxygen C. Sugar​

Answers

That would be A. Carbon dioxide.


I hope this helped!

I would appreciate it if you gave me a thanks and brainliest mark (:

Answer: A

Explanation:

During cellular respiration, a glucose molecule is gradually broken down into carbon dioxide and water.

1) Organisms reproduce either sexually or asexually. Asexual
reproduction is the reproductive process in which offspring have only
one parent. Sexual reproduction involves two parents who combine
their genetic material to produce a new offspring. Which of the
pictures show asexual reproduction
A) A&B
B) B&C
C) C&D
D) A,B, & C

Answers

Answer: NO BLOODY PICTURE. I never get the picture, diagram whatever, so why do I get the question?

Explanation:

HURRY I WILL GIVE BRAINLIEST AND VERIFIED ANSWER & 100 PTS Earthworms are a species of worms that lives in moist soil along with other organisms like tiny insects and bacteria. Earthworms obtain water and nutrients by eating dead plant material found in the soil. Earthworms also release waste materials that help enrich the soil. Based on this information, which sentence is correct? The earthworms are an environment. The earthworms are a community. Soil is the earthworm’s habitat. Soil is the earthworm’s niche.

Answers

Answer:

it has to be the last and 2nd last one of them

Explanation:

Sorry if this is wrong

What does it mean for a model to be "to scale", or a scaled model?

Answers

Answer:

To scale means it's the same size as in real life, as in 1 foot = one foot

Scaled means it's shown either larger or smaller, as in 1 foot = 1 inch or 1 foot = 1 mile

Explanation:

true or false , the mRNA codon UGG is the only codon that codes for the amino acid tryptophan

Answers

Answer:

true

Explanation:

How is Gypsy’s story related to psychology?

Answers

Answer:

Gypsy’s story is directly related to psychology.

Explanation:

Gypsy’s story is related to psychology because according to her mother who said that her daughter Gypsy Rose suffering from leukemia, and mental disorder but this claim of her mother proved wrong when the full health check up of Gypsy Rose was held. The doctors said that she was psychologically torture by her mother which leads to the killing of her mother.

how do scientists classify species?

Answers

Answer

d because that's what they causally do

Answer:

A

Explanation:

A P E X

research on obesity rates and genetically based skin color illustrate different types of biological change because

Answers

Explanation:

research on obesity rates and genetically based skin color illustrate different types of biological change because obesity rates demonstrate an evoked cultural response; genetically based skin color does not.

Which group of elements most readily combines with Group 17 elements?
a. Group 1
b. Group 17
c. Group 18
d. none, as Group 17 is the most stable group

Answers

Answer:

a. Group 1

Explanation:

Group 17 elements react most with group 1 elements because group 1 elements are metals.

PLEASE HELP ME ITS FOR 17 POINTS!!!!!!!!!!!!!!!!!!!!

A forest area is being used for a new housing development. Most of the trees have been removed to make room for the
new houses. Predict the impact that this will have on the birds in the area.
The bird populations will stay the same because humans love birds.
The bird populations will decrease because humans see them as pests.
© The bird populations will increase because humans do not interfere with wild animals.
The bird populations will decrease due to competition for resources such as food and nesting sites.

Answers

Answer:

The bird populations will decrease due to competition for resources such as food and nesting sites.

Explanation:

Their food and habit is being taken away so they will have to compete. I hope this helps you! :)

Possible Brainliest? You don't have to if you don't want to!

Which best describes the process of evaporation/transpiration in the
water cycle?
O A. Water from the ocean enters the atmosphere as water vapor.
O B. Water on the surface of Earth enters the groundwater supply.
C. Water vapor in the atmosphere returns to the surface of Earth as
liquid water.
O D. Water on the surface of Earth and water lost by plants enters the
atmosphere as water vapor.

Answers

Answer:

A

Explanation:

Answer:

The answer is D

Explanation:

Water on the surface of Earth and water lost by plants enters the atmosphere as water vapor

The person above me is wrong I tried it and it was wrong and I tried D and I got it correct

Which wavelength of light are more stretched—red light or blue light?

Answers

red light is more stretched

Answer:

Red

Explanation:

Blue light has a shorter wavelength than red.

After being hunted to near extinction, wolves were reintroduced to Yellowstone National
Park in 1995. After two years, the population of wolves increased rapidly until 2009.
After 2009, the population decreased slightly and has remained at an average of 93
wolves. Which type of growth curve describes the Yellowstone wolves?
A. population density curve
B. exponential growth curve
C. bell curve
D. logistic growth curve

Answers

Answer: B: exponential growth curve

Explanation: An exponential growth curve is J-shaped. Exponential growth is more common in R-selected species, which have a short life span and a high rate of reproduction. Yellowstone National Park has 124 wolves living in it.

3. What things does your body do automatically to cool down or warm up?

Answers

Answer:

For your body to cool down one method is to sweat.

Explanation: Your sweat glands release sweat, which cools your skin as it evaporates. This helps lower your internal temperature

To keep warm your body shivers and your blood pressure decrease, it brings the blood back to the heart to keep you warm, also in you blood, your hormones create glucungons that makes the liver shoot out glucose to keep you warm. To keep cool you body sweats and in you blood the hormones create something called insulin that makes you cell take in the glucose in you blood.(Fun Fact: the things that I just said are negative feedback).

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