When cloning eukaryotic genes into bacteria for protein production, a copy of DNA that lacks introns must first be obtained. Introns are removed from primary RNA transcripts by the cell's own machinery, but the copy of the DNA lacking introns must be generated by a biotechnology method. This intron-free copy DNA (or cDNA) is generated by

Answers

Answer 1
obtaining mRNA from the cell, then using reverse transcriptase enzyme to convert it into a stable cDNA (or copy of DNA) molecule for insertion into the bacterial cell.










I hope this helps!

Related Questions

7 DDT is a pesticide that was once widely used to control agricultural pests and
mosquitoes. However, this pesticide caused the eggshells of certain birds, including the
brown pelican, to become fragile and thin. The adults would then accidentally crush the
eggs while trying to incubate them.
What happened to brown pecan populations as a result of DDT use by humans?
A The populations increased much more slowly.
B The populations decreased as fewer eggs survived long enough to hatch.
C The populations remained stable over time.
D The populations increased as pelicans laid more eggs so that more offspring would
survive.

Answers

Answer:

The answer is B. The populations decreased as fewer eggs survived long enough to hatch.

Explanation:

In 1906, President Theodore Roosevelt created the Grand Canyon National Game Preserve on the Kaibab Plateau in northern Arizona. Between 1907 and 1923, cattle grazing was greatly reduced, mule deer hunting was eliminated, and predators were killed. Over 800 cougars, 20 wolves (most had already been killed in the 1800s), and 7000 coyotes were trapped or shot. In response, the mule deer herd began to increase: By 1915, deer numbers were estimated at 25,000; 50,000 by 1920; and 100,000 by 1923. The removal of the livestock and predators, and the cessation of hunting in 1907 ________. decreased the carrying capacity for the Kaibab deer decreased r, allowing more births among Kaibab deer decreased K, allowing more births among Kaibab deer removed the limits on immigration, allowing more deer into the area increased the carrying capacity for the Kaibab deer

Answers

Answer: increased the carrying capacity for the Kaibab deer

Explanation: The removal of livestock means more food as grass is available for the Kaibab deer to feed on. In the same vein, the nonexistence of predators would allow for proliferated growth which in turn would increase the number of deer population on the plateau. What this means is that the Preserve is now able to carry as much deer population as it could going forward thereby increasing the carrying capacity for the Kaibab deer which was evident by the increasing figures on the years the deer numbers were estimated.

Question:
1. Red blood cells:
A. Do not make ATP
B. Make ATP through glycolysis
C. Make ATP through the citric acid cycle
D. Make ATP through olvсolysis and the citric acid cycle
Dowmload Attachments.​

Answers

Answer:c.

Explanation:because atp is made threw the citric acid cycle

Please select the word from the list that best fits the definition

wheelchair ramp

Answers

Explanation:

A wheelchair ramp is an inclined plane installed in addition to or instead of stairs. Ramps permit wheelchair users, as well as people pushing strollers, carts, or other wheeled objects, to more easily access a building, or navigate between areas of different height.

Which of the following is an
example of active transport?
A. diffusion
B. moving molecules from an area of high to low
concentration
C. moving molecules against a concentration
gradient
D. moving molecules with the concentration
gradient

Answers

moving molecules with the concentration gradient

please help i will give you brainliest!!!!!!

Answers

Explanation:

you just have to sit there and basically say why the smog did what it did to the dinos, you need to sit there and explain how it hurt there bodys minds ect... its just like smoke to us it would make us choke from breathing it in and cause the black lung stuff.

Why does DMD affect mostly boys? Describe the type of inheritance pattern that DMD follows

Answers

Answer:

Inheritance in DMD

DMD is inherited in an X-linked pattern because the gene that can carry a DMD-causing mutation is on the X chromosome. Every boy inherits an X chromosome from his mother and a Y chromosome from his father, which is what makes him male. Girls get two X chromosomes, one from each parent.

Explanation:

Cite evidence that living systems follow the Laws of Conservation of Mass and Energy. How do living systems follow the law of conservation of mass and energy?

Answers

Answer: Living systems use the Law of Conservation of Mass when a living organism dies. When the organism decomposes carbon is released and enters back into the Earth. This follows the law because the mass is not being created or destroyed, but reused. Living systems use the law of conservation of mass when a living organism dies. ... It follows the law because the mass is not being created or destroyed, but reused. Living systems use the law of conversation of energy when an organism decomposes

Explanation:

HELPP ME PLZ I NEED HELP

Answers

Answer:

B. Bears hibernating in a cave during cold periods

Explanation:

Identify two two layers of earth where selsmic s waves do not travel
A.upper mantle
B.lower mantle
C.outer core
D.inner core

Answers

Answer:

Outer and inner core

Hope this helps!

A team of students encountered an unorganism in the field conducting a biodiversity study some students think the organism is a plant or other things organism which question should the students investigate to classify the organism correctly

Answers

Answer:

Does the organism perform photosynthesis?

Is your answer

Explanation:

Which of the following is a fossil fuel

Answers

Umm is there an image I can see what you are referring to?

7. What is the correct order of the levels of organization in the human body from
the simplest to the most complex?​

Answers

Answer:

The major levels of organization in the body, from the simplest to the most complex are: atoms, molecules, organelles, cells, tissues, organs, organ systems, and the human organism.

All living and non-living things are made of one or more unique substances called elements, the smallest unit of which is the atom, (for example, the element oxygen (O) is made of O atoms, carbon (C) is made of C atoms and hydrogen (H) is made of H atoms. Atoms combine to form molecules.

All living structures are made of cells, which are made of many different molecules. Cells are the smallest independent living thing in the human body. The body is made of many different cell types, each with a particular function, (for example muscle cells contract to move something, and red blood cells carry oxygen). All human cells are made of a cell membrane (thin outer layer) that encloses a jelly-like cellular fluid containing tiny organ-like  structures called organelles.

A tissue is a group of similar cells that work together to perform a specific function. There are four main tissue types in humans (muscular, epithelial, nervous and connective). An organ is an identifiable structure of the body composed of two or more tissues types (for example, the stomach contains muscular tissue made of muscle cells, which allows it to change its shape, epithelial tissue which lines both the inner and outer surface of the

stomach, nervous tissue which sends and receives signals to and from the stomach and the central nervous system, and connective tissue which binds everything together).

Organs often perform a specific physiological function (for example, the stomach helps digest food). An organ system is a group of organs that work together to perform a specific function (for example, the stomach, small and large intestines are all organs of the digestive system, that work together to digest foodstuff, move nutrients into the blood and get rid of waste). The most complex level of organization, the human organism is composed of many organ systems that work together to perform the functions of an  independent individual.

hope this answer helps you.....

please mark as brainliest .... thank you!

Name three different alleles for human hair color

Answers

Answer:

At least two genes are involved in hair color. One hair color phenotype (brown vs. blond) has a dominant brown allele and a recessive blond allele. A person with a brown allele will have brown hair; a person with no brown alleles will be blond.

Explanation:

Answer:

BB, Black, Bb, brown, bb blonde

Explanation:

QUESTION 39
According to the lecture, which structure of the ear did the professor associate with possible mathematical genius?
Cochlea
Semicircular canal
O Stapes
o Tympanic membrane

Answers

Answer:

Cochlea is the right answer

For this question, use the fact that pea plants use simple dominant inheritance patterns. T = tall trait, t = short trait.
heterozygous tall plant is crossed with a heterozygous tall plant. What percent of the offspring will be short?
A 0%
B 25%
C 50%
D 75%
E 100%

Answers

The answer is B 25%

The work is down below if you need it:)

Order smallest to largest.

organ, cell, tissue, body system

Answers

Answer:

Explanation:

cells, tissues, organ, and body system.

cell, tissue, organ, body system

1. Name the living things that make food for themselves and provide food for others

also ______​

Answers

Answer: An Autotroph.

Friction reduces efficiency because energy is lost as ____.
a.
work
c.
gravity
b.
heat
d.
thrust


Please select the best answer from the choices provided

A
B
C
D

Answers

Answer:

B

Explanation:

b. heat

which two method of waste management do you believe are best for society?​

Answers

Answer:

The two methods of Waste Management which I believe are best for society is recycling and landfilling.

Answer:

gsgshahhsudgdhxhciauahaususisssivdvdheusu

When the North Pole is farthest away (tilted away) from the sun it is possible for areas within the Artic circle to experience 24 hours of darkness without any sunlight.
A. True
B. False

Answers

Answer:

I think true

Explanation:

mark me brainliest

which trophic level of the energy pyramid does the anchovy belong?
A: 1: producer
B: 2: Primary consumer
C: 3: Secondary consumer
D: 4: Tertiary Consumer

Answers

The answer is D I just took the test

If you had to prioritize the removal of plants from an area , rank the following in order of highest to lowest priority by dragging them in the correct order top to bottom .
native non - aggressive
non - native non - aggressive
non -native aggressive
native aggressive​

Answers

Answer:

non -native aggressive

non - native non - aggressive

native aggressive​

native non - aggressive

Explanation:

Non native plants are always the worst for the area, so they go first, followed by native and aggressive plants.

Plants are living organisms, which are found in different regions. The plants can be native to a given area, can be non-native,  or aggressive.

The correct sequence is:

non -native aggressive

non - native non - aggressive

native aggressive

native non - aggressive

The plant species can be described as:

Non-native species of plants are described as the plants that cause economic and ecological losses in a given area. These plants are the worst and can hamper the natural ecosystem of the given location.

Native species are defined as organisms that have occurred naturally in a particular location or habitat. These species are great for a given ecological location and maintain a stable ecosystem.

Aggressive species or invasive species are defined as the species that occupy the natural habitat of organisms very fast. these species are unwanted and can negatively affect an ecosystem.

Thus, the native, non-native, and invasive species are different types of organisms that can either benefit or harm the ecosystem.

To know more about plant species, refer to the following link:

https://brainly.com/question/4059233

**25 POINTS** DNA is an organic molecule that is the code of life. DNA is made up of smaller molecules that form together into two chains. Those chains spiral around each other to form the DNA molecule. Which dichotomous key best describes DNA?

Answers

Answer:

2

Explanation:

A cohort study of benzene exposure and cancer was conducted in a tire manufacturing company:________
a) There were a total of 1,250 people working for the company.
b) 540 of the employees were exposed to benzene as a part of their daily job duties.
c) 710 employees were not exposed to benzene during their job duties.
d) 52 of the employees exposed to benzene developed cancer
e) 16 of the employees not exposed to benzene developed cancer.

Answers

In the  given question these are not options but the numbers of individuals affected or present in the study, the main question is:

calculate and interpret the relative risk for this study?

Answer:

The correct answer is - 4.27.

Explanation:

Given:

The total population is = 1250

                        Diseased.                      Healthy

Exposed              52...(a)                         488...(b)

Not exposed.       16...(c)                       694...(d)

solution:

Incidence among exposed / Incidence among non-exposed.

=> [tex]\frac{a}{a+b}[/tex]÷[tex]\frac{c}{c+d}[/tex]

=> 52/540÷16/710

=> 52×710÷16× 540

=> = 4.27

Thus, the Relative risk is = 4.27

5) Which of the following does not occur during the light reactions of photosynthesis?
A) Oxygen is split, releasing water
B) Electrons from chlorophyll are added to an electron transport chain
C) An electron transport chain drives the synthesis of ATP for use by the Calvin cycle
D) NADPH is produced and will carry electrons to the Calvin cycle
E) Oxygen is produced when water s split

Answers

Answer:

A)

Explanation:

Oxygen doesn't split for water, water splits for oxygen

Does the carbon cycle in this diagram appear to be in balance or out of balance? Use specific evidence from the diagram to support your answer. This diagram shows gigatons of carbon moving to and from the atmosphere each year.

Answers

Yes the diagram is valences

This diagram shows gigatons of carbon moving to and from the atmosphere each year and yes the diagram is valences.

Which factor is responsible for fixation?

Cutting down large areas of forests Plants are responsible for fixation of atmospheric carbon dioxide. If the number of plants is reduced, the amount of carbon dioxide moved from the atmosphere to vegetation will also decrease.

The biogeochemical cycle in which carbon is exchanged between the biosphere, pedosphere, geosphere, hydrosphere, and atmosphere of the Earth is known as the carbon cycle.

According to the question, emitted amount of carbon is 480 gigatons but the amount of carbon in the environment is approx 190 gigatons, so the remaining amount of the carbon was stoored by the oceans and other water reservoir, which may be get clear throug the attached diagram.

Therefore, This diagram shows gigatons of carbon moving to and from the atmosphere each year and yes the diagram is valences.

Learn more about atmosphere on:

https://brainly.com/question/28310375

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Which of these energy resources is sustainable? A. Wind O B. Oil O C. Coal O D. Biofuel​

Answers

Answer:

A. Wind.

Explanation:

Energy resources which are sustainable are the ones which would never run out. Wind is something that won't run out because of the windmills in several countries used to generate electricity. Oil, coal and biofuel are limited resources and isn't sustainable at all.

The energy resource that is sustainable from the given options is A) Wind. It is used in various ways of the energy production as well.

Biofuels can be sustainable, but it depends on how they are produced. Biofuels made from sustainable sources such as waste materials or non-food crops are considered sustainable.

However, biofuels made from food crops can contribute to deforestation, food shortages, and other environmental and social issues.Overall, Wind energy is the most sustainable option from the given choices as it is renewable and environmentally friendly.

Learn more about biofuels    at :

https://brainly.com/question/502679

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Energy from the wind is used to turn windmills. These windmills then spin a generator to produce electricity. Which statement would NOT support the claim that wind energy is considered a clean, renewable resource.

a. Wind energy is produced naturally.
b. Wind energy is used without giving off greenhouse gases.
c. There is an abundant supply of wind energy available for use.
d.There are only certain places that are suitable for collecting wind energy.

Answers

Answer: d

Explanation:

I think it is d

In order for an object to sink, its density must ___________ 1g/ml.

a.
be less than
c.
be equal to
b.
be greater than
d.
be double



Please select the best answer from the choices provided


A
B
C
D

Answers

Answer:

B

Explanation:

If its greater than it will have a better chance at sinking because of the mass

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