what is the purpose of sub culturing​

Answers

Answer 1

Answer:

In biology, a subculture is a new cell or microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculture is used to prolong the life and/or expand the number of cells or microorganisms in the culture.


Related Questions

How does epithelial tissue respond to a bruise or wound?

Answers

Answer:

Epithelial tissue helps bruises and wounds by reducing the risk of infection.

Explanation:

How did the death of chestnut tree result in the growth of the ecosystem

Answers

Answer:

add nutrients to the soil.

Explanation:

The death of chestnut tree result in the growth of the ecosystem because when the chestnut tree dies, the decomposers such as fungi and bacteria start feeding on the dead tree and as a result nitrogenous waste is produced mixed with soil which is the essential nutrient for the plants. These nitrogenous waste is used by the plants to make amino acids and as a result the growth of an ecosystem occurs.

PLSSSS 30 POINTSS BRAINLEST

Answers

Answer: The attachment listed some cell organelles,although there isn't a specific question on then but am going to be defining each of them and state their functions as well.

1. The Nucleus; The is command centre of the cell, it is made up of nuleolus, chromosomes, nucleoplasm,nuclear envelope and nuclear lamina.

it contains the genetic/hereditary materials that the cell is made up of.

The Nucleus plays a great role in the control of growth and development of the cell.

The nucleus also coordinates some of the the activities of the cell like cell division and protein synthesis.

2. Cytoplasm; The cytoplasm is one of the cell organelles,it is the liquid portion of the cell,it consists of cell contents between the plasma and the cell membrane. The fluid cytoplasmic material is where many cell organelles are found/suspended fluid. Most activities carried out by the cell occurs in the cytoplasm.

3. Endoplasmic Reticulum; The Endoplasmic Reticulum is one of the cell organelles that have the structure of a network of flattened sacs and tubules and play a crucial role in plant and animal cell. Rough endoplasmic reticulum has ribosomes attached to the cytoplasmic side of the membrane and the smooth endoplasmic reticulum lacks attached ribosomes in them thus giving it the smooth appearance.

The Endoplasmic Reticulum functions in the production, processing, and transport of proteins and in and out of the cell and also the production of lipids.

4. Golgi Body; One of the plant organelles that plays a role in the sorting and organizing the proteins produced and processed by the Endoplasmic Reticulum. these proteins are sorted and packed in vessicles to be used by the body.

5. Mitochondria; (a double membrane organelle). It is otherwise known as the powerhouse of the cell,it produce/generate energy in the form of ATP needed for cellular respiration. These energy produced is used for other cellular metabolic activities.

6. Lysosome (Animal) Chloroplast (Plant); Lysosomes are found in most animal cells, they function by digesting good and garbage,they contains acids that aids this function in the stomach of the cell and when the lysosome is diseased or explodes,the cell stops functioning.

The Chloroplast on the other hand is found in the cells of plants and algae,they play a crucial broke in photosynthesis by capturing the energy from the sun and turning the captured energy into usable forms like sugar,glucose and other organic molecules.

7. Cell Membrane; The cell membrane is the cell organelle that forms a boundary between the cell and the sorounding environment.

The cell membrane as well controls the movement of cellular materials in and out of the cell.

8. Nucleolus; The nucleolus is one of the cell organelles where ribosomes are made. The ribosomes are small grain-shaped organelles that make protein and they are found in the surface of rough Endoplasmic Reticulum. Nucleolus are small and round in structure and are found in the nucleus,

9. Vacoule (Plant Cells) Centrioles (Animal Cells); The vacoule is a storage chamber(reservoir) for wanted and unwanted materials in the cell, the vacoule is found within the cytoplasm and it is enclosed by a membrane that typically contains fluid.

The centrioles on the part of the animal cell is a cell organelle that has a structure of a two hollow cylinders,ring of microtubules next to each other(the centrosome).the centrioles are located just outside the nucleus of the cell.

Centrioles function by producing the mitotic spindles for cell division, and also assisting to pull chromosome pairs apart to opposite end of the cell during cell division.

10. Cell Wall; A protective semi permeable rigid outer layer of nonliving material that surrounds the cells of plants and some other organisms.

The cell wall houses the organelles of the plant cell.

The cell wall functions by giving the cell strength and structure,it's semi permeable nature filters and selectively allows certain materials in and out of the cell.

Answer:

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Explanation:

4. In the background, the presence of naturally occurring enzymes in fruits and vegetables was discussed. Using your results from this exercise as a basis to guide your thoughts, do you think that cooked, frozen, or raw fruits and vegetables would contain the most active enzymes?

Answers

Answer:

The fruits inside do not have enzymes, but if our body when digesting them, if these fruits are cooked they are much easier to digest by our digestive enzymes and if they are raw, digestion will be more laborious since it will have to break more masses chemicals that were not destroyed by cooking.

Regarding freezing, it does not intervene in digestion.

Explanation:

The cooking of vegetables or fruits like meats generates that some chemical unions are destroyed, thus exposing the most internal structures in enzyme activity.

Caffeine prolongs and intensifies the effects of epinephrine. Which of the following potential mechanisms of action of caffeine is consistent with this effect?
a. inhibits cAMP phosphodiesterase, the enzyme that converts cAMP to AMP.
b. a competitive antagonist of cAMP binding to PKA.
c. stimulates phospholipase Cb.
d. inhibits sarcoplasmic/endoplasmic reticulum Ca 2 + - ATPase.
e. activates PKC.

Answers

Answer:

a. inhibits cAMP phosphodiesterase, the enzyme that converts cAMP to AMP.

Explanation:

The phosphodiesterases are a group of enzymes that are capable of breaking phosphodiester bonds such as those between nucleotides in nucleic acids. The Cyclic adenosine monophosphate (cAMP) is a second messenger involved in intracellular signaling pathways. This molecule (cAMP) is hydrolyzed by the cAMP-dependent phosphodiesterase, which catalyzes the enzymatic breakdown of phosphodiester bonds (i.e., hydrolyzing cAMP to 5-AMP). The cAMP levels are modulated by the balance between its generation and the degradation via cyclic nucleotide phosphodiesterase.

Que son las células procariotas?

Answers

Answer:

En biología, con el término procariotas se hace referencia a las células que no tienen un núcleo formado, en contraste con las células que han formado un núcleo, las células eucariotas.

Estas células tienen una estructura más simple que las células eucariotas y son de menor tamaño.  Externamente, las células procariotas están separadas del medio ambiente por la membrana celular, pero internamente no tienen ninguna otra formación. En su mayor parte, los organismos procariotas son unicelulares y pertenecen al grupo de bacterias.

which is a type of star system?

A. dim stars

B. galaxies

C. wobbling stars

D. globular clusters

Answers

Answer:

D) Globular Clusters

Explanation:

A star system or stellar system is a small number of stars which orbit each other, bound by gravitational attraction. A large number of stars bound by gravitation is generally called a star cluster but, broadly speaking, they are also star systems. A star cluster is a group of stars that share a common origin and are gravitationally bound for some length of time. The two basic categories of stellar clusters are open clusters, also known as galactic clusters, and globular clusters.

Globular clusters is a type of star system.

What is star system?A star system, also known as a stellar system, is a group of stars that circle each other and are held together by gravitational pull. A stellar cluster or galaxy is a huge group of stars that are gravitationally bonded together, however they are also known as star systems. Planetary systems, which comprise planets and other similar bodies, are not to be mistaken with star systems such as comets.What is globular clusters?Globular clusters are clumps of old stars that are firmly packed together. They have a roughly spherical form and contain hundreds of thousands, if not millions, of stars. Astronomers can use them to determine the age of the cosmos or the location of a galaxy's core by studying them.

Hence, the correct option is D.

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Consider the following DNA molecule: 5' TTC CAT GGA 3' 3' AAG GTA CCT 5' If this molecule were transcribed, what would be the resulting RNA molecule

Answers

Answer:

DNA strand: 5' TTC CAT GGA 3'

RNA strand: 3' AAG GUA CCU 5'

Explanation:

From the DNA template, an RNA molecule will be transcribed that is single stranded. So, it will read off the code from the 5' to 3' strand of DNA to create the RNA molecule made of complementary bases, in triplet codons. The enzyme that carries out this function is RNA polymerase.

he effect of autonomic fibers on target cells is _________. The effect of somatic fibers on target cells is __________.

Answers

Answer:

The effect of autonomic fibers on target cells is excitatory or inhibitory

The effect of somatic fibers on target cells is always excitatory.

Explanation:

The autonomic fibers is a part of peripheral nervous system that controls the involuntary actions of internal organs and glands like heart and intestines.

It's effect are excitatory or inhibitory because reflexive and involuntary manner. It helps to maintain homeostasis (internal stability and balance) through the coordination of various activities such as hormone secretion, circulation, respiration, digestion and excretion. This system control fight to fight response in the body incase of danger.

This system operate in opposite" actions where one system activates a physiological response and the other inhibits it.

The somatic fibers are part of the peripheral nervous system that help transmit sensory information and motor information. They are made up of nerves that connect to the skin, sensory organs, and all skeletal muscles.

Somatic neurons are excitatory neurons because excite their target postsynaptic neurons or target cells causing it to function..

Jose Salazar has been hospitalized following a motor vehicle accident. He has several broken bones, including his left jaw, two ribs on the left side, and a compound fracture of his left leg. According to his medical record, he is 47 years old, his height is 5'9", and his weight is 171 pounds. During the course of his hospital stay, he was diagnosed with a heart arrhythmia. His current medications include lovastatin to lower his cholesterol, an MAO inhibitor to treat depression, and an analgesic to treat his pain. Mr. Salazar's wife also reports that he takes several herbal products including yohimbe, kava, valerian, and St. John's wort. Which herbal product taken by Mr. Salazar may be causing his heart arrhythmia?a. yohimbeb. kava kava onlyc. St. John's wortd. valerian onlye. both valerian and kava kava

Answers

Answer:

a. yohimbeb

Explanation:

Heart arrhythmia is a heart disease in which the heart does not work properly. The main cause of heart arrhythmia is yohimbeb which is used as a medicine for increasing sexual desires but this herbal product also has side effects such as causing heart problems such as heart arrhythmia and kidney diseases. So the Jose Salazar should avoid the consumption of yohimbeb in order to control the heart disease.

Question 21 (5 points)
Which of the following terms describes a collection of hollow cells that implants on
the uterus wall after fertilization, but is not yet differentiated (in other words, all cells
are the same type of cell still)?
Fetus
Haploid
Mitosis
Blastocyst

Answers

Answer:

The answer is option D.

Blastocyst

Hope this helps

Answer:

Blastocyst

Explanation:

Once an egg is fertilized by a sperm, it begins to divide by mitosis into a 2, 4, and then 8 celled structure. Eventually, this large collection of cells keeps dividing and becomes a hallow collection of undifferentiated cells called a blastocyst. The blastocyst will implant on the wall of the uterus where it will begin to grow and develop during pregnancy into a fetus.

What are gyres?
1.Large plants that move in circles in the ocean.
2.Large circulating parts of the ocean
3.Large animals in the ocean that move in circles in the ocean.

Answers

Answer:

A gyre is a circular ocean current formed by the Earth's wind patterns and the forces created by the rotation of the planet..

Explanation:

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Why are all food service workers required to understand and apply food safety knowledge?

Select one:
a. To prepare the best tasting food possible.

b. To prevent the spread of illnesses through food.

c. To reduce customer injury such as cuts and burns.

d. To reduce the amount of food thrown away due to spoilage.

Answers

Answer:

B.

Explanation:

Keeping and understanding food safety knowlege prevents the consumer from getting sick.

About Galileo Galilei : Why was he allowed to serve his sentence from home?​

Answers

Early in 1616, Galileo was accused of being a heretic, a person who opposed Church teachings. ... Galileo was sentenced to life imprisonment in 1633. Because of his age and poor health, he was allowed to serve his imprisonment under house arrest.

Explanation:

a. What are the two genotypes of the grandparents? ________ b. What is the genotype of Jane's husband? ________________ c. What are the chances that Peter is the carrier of the PKU allele that resulted in his having an affected son? _________%

Answers

Answer:

a. Rr, Rr

b. rr

c. 0% because its the women who transmits the disorder.

Explanation:

PKU allele is an autosomal recessive disorder which is caused by mutations in both alleles of gene for phenylalanine hydroxylase. Alleles are either dominant or recessive. When two alleles are identical means that mother and father cells are homoozygous recessive. They both are combining the same alleles causing it to have a higher chance of genetic disorder.

Air pollution in national park is effecting the environment in which way

Answers

Answer:

Nature

Explanation:

Air pollution harms plants and animals and even contaminates water – it stifles tree and crop growth, causing leaves of tree species to blacken and wither.

What allows enormous lengths of DNA to
be folded inside the nucleus and prevents
chromatin from becoming tangled
during mitosis?
A. chromosome
B. ribosome
C. centrioles
D. nucleosome

Answers

The answer is D a nucleosome; a nucleosome has DNA wrapped around histones which prevents chromatin from tangling :)

Answer:

D

Explanation:

In Pennsylvania, wolves have been extinct for many years. They used to feed on white-tailed deer, but not that the wolves are gone, the population of white-tailed deer has exploded. This means that this community:______

a. Was under top down control
b. Was under bottom up control
c. Lacks a fundamental niche for wolves
d. Lacks a fundamental niche for deer

Answers

Answer:

The correct answer is a: The community was under top-down control

Explanation:

Top-down control refers to scenarios where superior predators control the structure and dynamic of an ecosystem. These systems are affected by the presence/absence of the superior predator influences all the lower links in the trophic chain.  

In the exposed example wolves, that are considered to be the top predators, affected the white-tailed deer populations being the cause of its decrease.  As wolves used to feed on white-tailed deer, they kept these populations under control, but once the wolves got extinct, the deer population increased again. Probably, this explosion in the deer´s population also affected its lower link in the trophic chain.

 

What can a line graph be used to do? Select the best answer.
O A. Identify positive correlation
B. Identify negative correlation
C. Make predictions
D. Make comparisons
Reset Selection

Answers

Answer:

From the choices listed I would say that the answer is (D) make comparisons.

Explanation:

Line graphs are used to track changes over short and long periods of time. Line graphs are used to compare changes over the same period of time for more than one group.

A line graph is used to make predictions. Thus, the correct option is C.

What is a line graph?

A line graph may be defined as a kind of chart utilized to illustrate the information that altered over time.

Identification of positive correlation and negative correlation is done by using a scatter plot.

A Line graph is extremely an easy process to make predictions because it illustrates the alterations over a specific period of time.

Therefore, the correct option for this question is C.

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Assume that a cell is carrying out its day-to-day activities. At one point, the nucleotides GAT are paired with the nucleotides CUA. This pairing occurred __________.

Answers

Answer:

Explanation:

Yes!

If evolution is based upon there being genetic diversity between generations, how does the lack of genetic differences existing between daughter cells created via mitosis impact the theory of evolution

Answers

Answer:

See the answer below

Explanation:

If evolution is based upon genetic diversity, it means that evolution in organisms that only reproduce by mitotic division will depend largely on the frequency of random mutation of genes.

The only way that genetic diversity can occur between daughter cells formed from mitosis is if mutation takes place. Mutation general means a sudden change to the gene sequence of an organism.

Without mutation, evolution in mitotic organisms will be non-existence because there are no new genes or alleles being introduced into the gene pool.

The central (Haversian) canal that runs through the core of each osteon is the site of ________. adipose tissue and nerve fibers cartilage and interstitial lamellae blood vessels yellow marrow and spicules

Answers

Answer:

Blood vessels and nerve fibers

Explanation:

The Haversian canal serves as the bone tissues and it runs through the core of each osteon. The osteon surrounds the Haversian canal and contain structures called lamellae.

These lamella are made largely of collagen fibers and also various network of rich blood vessels and cells which produces blood cells in the body. Nerve cells which helps in the relay of impulses are also present. The rich contents of the bone gives it its strength and durability.

Answer:

The correct answer is: blood vessels and nerve fibers.

Explanation:

Haversian Canals are a group of tubes that are located in the more peripheral region of the bone, known as the cortical bone. The cortical bone is hard and compact and is composed of several concentric layers of bone cells (osteocytes and osteoblasts) that circle around a haversian canal, which contains capillaries and nerve fibers. This structure is called an osteon.

Haversian Canals are very important for the correct functioning of the bone, as they give the cells their needed blood supply and innervation.

what is cellular affinity in biology?

Answers

Answer:

affinity. Attraction between particles. Biology: A measure of the attraction of one biological molecule toward another molecule, either to modify it, destroy it, or form a compound with it. Examples are enzymes and their substrates, or antibodies and their antigens.

The highlighted structure contains part of the brain system that maintains cortical alertness. What is the name given to this brain system

Answers

Answer:

Reticular formation

Explanation:

The Reticular formation consists of nuclei which are connected together. They are found in the Brain Stem. The Brain stem and Reticular formation are also both found in the Medulla Oblongata.

They however contain rich volume of cells which helps in the cortical alertness and they also help in the control of the activities of the skeletal and some parts of the muscular system.

What can carbon atoms do that makes them different from atoms of the five
other common elements of life?
A. Form compounds with nitrogen and phosphorus
B. Form compounds with hydrogen and oxygen
C. Form polyatomic ions
D. Form four covalent bonds

Answers

Answer:

B. is the answer

Explanation:

form compounds with hydrogen and oxygen .

Answer:

Form four covalent bonds

Explanation:

Carbon makes four electrons available to form covalent chemical bonds, allowing carbon atoms to form multiple stable bonds with other small atoms, including hydrogen, oxygen, and nitrogen.

describe some of the basic trends in hominid evolution (brain size, jaw size, language, and manufacturer tools) using at least five sentences.

Answers

The five trends of hominid evolution examined by paleoanthropologist are the advent of a bipedalism subsequent exploration and terrestrial habits increase the brain size, the use of tools, and the growing proportions of meat protein in hominid diets

Bile is formed by

a. sinusoidal endothelium

b. stromal cells

c. lymphocytes
d. (von) kupffer cells
e. hepatic cells (hepaticytes) ​

Answers

Answer:

E. hepatic cells (hepatocytes) ​

Explanation:

These cells are the ones that secrete bile acids, rest do not match the given description

These cells make up about ~70-80% of liver mass and are primary authority at producing bile for the body

Answer:

Bile is formed from (con) kupffer cells

Why is the "dorsal-ventral" axis not synonymous with the "posterior-anterior" axis in the whole human nervous system?

Answers

Answer:

The ventral side is towards the belly side

The Dorsal side is back to the belly side

Anterior is Before the belly side

Posterior is after or coming after to the belly side.

Explanation:

The ventral side is associated with the animal or a person belly side which related to the organism digestion and respiratory organs.

The anterior is the before or in front of side position which is associated with the front line of an organism.

The posterior side is related to the after side or it is opposite to the anterior position of an organism.

It is depend on the organism position, that these sides are related to each other in some instances. In some cases these are orthogonal in some times.

which animal look very different when it is grown up from when it was young

Answers

Answer:

Explanation:

Animal which looks very different when it is grown up from when it was young is a tadpole.

Hope this picture is clear and easy to understand

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In the intrinsic pathway, the reaction begins with clotting factors from the _.

Answers

Answer:

Clotting factors are circulating in the blood.

Explanation:

In the intrinsic pathway, the body is responding intravascular damage through binding of platelets, exposure of chemicals or even collagen from endothelial membranes. The most of the clotting factors are synthesized by the liver and circulate around the blood stream until they are needed or until they are degraded based off their half lives.

When the platelets start to aggregate they release chemicals and have proteins that can attract the clotting factors. In the intrinsic pathway, clotting Factor XII starts off the cascade. Hope that helped :)

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Johan is the manager of CycleUp and has agreed to build a new manufacturing plant in Argentina. He realizes this decision will cause him to shut down a small plant in Ohio, but he decides that since the new facility will cut his operating costs in half, that is worth more than closing a plant employing ten people. Which philosophical approach to ethics does this demonstrate For vulnerable road users, the areas where roadways transition from one environment to another are...A. the safest areas.B. the most clearly marked areas.C. the most dangerous areas. Under which model of oligopoly are firms more likely to engage in a price war, driving down prices to marginal cost and resulting in no profit? According to Nikki, the design and development manager at Holden Outerwear, it is difficult for the company to push its vendors toward new developments because the vendors: Round7.8652to 2 decimal places. Please help!! Tamar is measuring the sides and angles of Triangle TUV to determine whether it is congruent to the triangle below. How many x-intercepts does the graph of y = 2x2 + 4x - 3 have? 3/7 of which is 2 1/14 A cube with 40-cm-long sides is sitting on the bottom of an aquarium in which the water is one meter deep. (Round your answers to the nearest whole number. Use 9.8 m/s^2 for the acceleration due to gravity. Recall that the weight density of water is 1000 kg/m^3.) Which method is the main feature of a Zoroastrian funeral?O A.burning the dead body on a funeral pyreOB.burying the dead body in sacred ground.enclosing the dead body in a casket and placing it in a cremation chamberOD.burying the dead body in a deep well filled with a mixture of charcoal and limeO E. allowing the dead to be consumed by flesh-eating birds Write a description suggested by this picture. (Think about areas to zoom in on to describe in detail.) 2. The change in internal energy for the expansion of a gas sample is -4750 J. How much work is done if the gas sample loses 1125 J of heat to the surroundings? Is this work done by the gas or done by the surroundings? the time required for one cycle, a complete motion that returns to its starting point, it called the_____. period medium frequency periodic motion An organism is found that has the following traits: Has multicellular filaments Decomposes plants Lives in a moist environmentWhat kingdom does this organism most likely belong in?O A. AnimaliaO B. PlantaeO C. ProtistaO D. Fungi When4a- a x 3a + 7ais simplified it is equivalent to A chemistry student weighs out of ascorbic acid , a diprotic acid, into a volumetric flask and dilutes to the mark with distilled water. He plans to titrate the acid with solution. Calculate the volume of solution the student will need to add to reach the final equivalence point. Round your answer to significant digits. A dilation has center (0, 0). Find the image of each point for the given scale factor. A(3,4);D7(A) On January 1, 2013, an investor purchases 25,000 common shares of an investee at $9 (cash) per share. The shares represent 20% ownership in the investee. The investee shares are not considered "marketable" because they do not trade on an active exchange. On January 1, 2013, the book value of the investee's assets and liabilities equals $600,000 and $150,000, respectively. On that date, the appraised fair values of the investee's identifiable net assets approximated the recorded book values. During the year ended December 31, 2013, the investee company reported net income equal to $22,500 and dividends equal to $12,000.Noncontrolling investment accounting (price equals book value) Assume the investor does not exert significant influence over the investee. Determine the balance in the "Investment in Investee" account at December 31, 2013. Which option is an example of inductive reasoning ?A. Poodles are dogs. Sparky is a poodle. Therefore, Sparky is a dog. B. She keeps winning coin tosses. Therefore she will win the next one.C. Storms come every may. Therefore, storms will not come next may. D. Lemons are fruit. Apples are fruit. Therefore apples are lemons. Pls answer will mark you brainliest!! which situation is most likely to show a constant rate of change