What are the advantages and disadvantages of the serial dilution-agar plate procedure?​

Answers

Answer 1

Answer:

Advantages

- Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies.

- In biochemistry, serial dilution is used to obtain the desired concentration of reagents and chemicals from a higher concentration.

- In pharmaceutical laboratories, serial dilution is performed to receive the necessary concentration of chemicals and compounds as this method is more effective than individual dilutions.

- In homeopathy, homeopathic dilutions are used where a substance is diluted in distilled water or alcohol. It is believed than dilution increases the potency of the diluted substance by activating its vital energy.

Disadvantages

- An error might occur during the propagation of the sample, and the transfer inaccuracies lead to less accurate and less precise transfer. This results in the highest dilution to have the most inaccuracies and the least accuracy.

- Because serial dilution is performed in a stepwise manner, it requires a more extended period of time which limits the efficiency of the method.

- Serial dilution only allows the reduction of bacteria/cells but not the separation of bacteria/cells like in other techniques like flow cytometry.

- This technique also requires highly trained microbiologists and experts in aseptic techniques.


Related Questions

which one contains many organelles with different functions prokaryotic cell eukaryotic cell​

Answers

Answer:

Eukaryotic Cell

Explanation:

Eukaryotic cells contain membrane-bound organelles, such as the nucleus, while prokaryotic cells do not. Differences in cellular structure of prokaryotes and eukaryotes include the presence of mitochondria and chloroplasts, the cell wall, and the structure of chromosomal DNA.

eukaryotic cells is the answer

A group of students used this diagram to classify three organisms into different kingdoms which table identifies the three kingdoms

Answers

Answer:

Answer is c. fungi, protista and plantea

Of the parts of the electromagnetic spectrum listed below, which has the shortest wavelength?
A.
visible light
B.
infrared radiation
C.
radio waves
D.
X-rays

Answers

radio waves. hope this helps!

what are the the four main categories of organic macromolecules ?

Answers

Answer:

There are four major classes of biological macromolecules (carbohydrates, lipids, proteins, and nucleic acids), and each is an important component of the cell and performs a wide array of functions.

Four main categories of organic macromolecules are as follow:

carbohydratelipidsproteinsnucleic acidWhat are macromolecules?

Macromolecules are polymers containing long chains of molecular sub-units known as monomers. These play an important role in biophysical processes and composed of thousands of atoms that are covalently bonded.

Carbohydrate is a biomolecule having carbon, hydrogen and oxygen atom.It is the main source of energy of the body.

Lipids are macromolecules that are insoluble in water and the main function is to serve as structural component of cell membrane.

Proteins are macromolecules that are extremely complex in structure and made up of amino acid residues joined by peptide bonds.

Nucleic acids are biopolymer macromolecules and composed of nucleotide. The function is to carry genetic information which helps to make RNA.

Therefore,Four main categories of organic macromolecules are as follow:

carbohydratelipidsproteinsnucleic acid

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Which of the following tropisms are the
same as geotropism?
a. thigmotropism
b. hydrotropism
c. phototropism
d. gravitropism

Answers

Answer:

d

Explanation:

d.gravitropism as it's both going downwards

The average molecular weight of proteins encoded in the human genome is about 50,000. A few proteins are very much larger than this average. For example, the protein called titin, which is made by muscle cells, has a molecular weight of 3,000,000. Estimate how long it will take a muscle cell to translate an mRNA coding for an average protein and one coding for titin. The average molecular mass of amino acids is about 110 daltons. Assume that the translation rate is two amino acids per second.

Answers

Answer:

13,636 seconds = 227.3 minutes = 3.79 hours

Explanation:

The titin protein has a molecular weight of 3,000,000 daltons, while the average molecular mass of one amino acid is 110 daltons, so it is expected that this protein is composed of 27,273 amino acids (3,000,000 daltons/110 daltons = 27,272.72). Moreover, and since the translation rate is two amino acids per sec, it is expected that the protein will be synthesized in approximately 3.79 hours (27,272.72/2 = 13,636 seconds = 227.3 minutes = 3.79 hours).

competition a type symbiotic relationship is where

Answers

Answer:

they r ecological, not symbiotic

The text box below contains facts about salmon:
Facts about salmon. baby salmon migrate from freshwater streams and rivers to the ocean.
in the ocean, baby salmon d/r/i//n/k salt water.
Special cells on the salmon's gills called c/h//l/o//r/i/d//e cells help the salmon to e/x/c/r//e/t//e/ salt.

How do populations of baby salmon respond to changes in s/a//l/i//n/i//t/y as they migrate?
The baby salmon u/t/i/l/i//z/e// special structures but do not a/l/te/r/ their behavior.
The baby salmon al/t/e/r/ their behavior and u/t//i/l//i/z/e special structures.
The baby salmon u/t/i//l//i//z/e special structures to a/l/t/e//r their behavior.
The baby sa.l.m.on a/l/t/e/r their behavior but do not u/t/i/l//i/z/e special structures.

Answers

Answer:

what even is the question i am so cunfuzzles

Some people's earlobes dangle or flap unattached, while others don't. This trait is genetically determined. Use the genetics simulation to explore the genes that determine whether earlobes are attached or unattached. One trait is dominant and the other is recessive. See what happens when you cross parents with different genetic combinations. Follow the specific instructions for each trial.

Answers

Complete question, Answer, and Explanation:

Due to technical problems, you will find the complete question, answer, and explanation in the attached files

An igneous rock has a coarse texture and is dark in color. How else can this rock be accurately described?

small crystals
cooled quickly
produced from magma
formed at Earth’s surface

Answers

Answer:

C. produced from magma.

D. formed at Earth’s surface

Explanation:

Earth crust refers to the outermost layer or thin shell which makes up the earth such as sedimentary, metamorphic and igneous rocks.

The Earth crust is typically made up of two (2) main types;

1. Oceanic crust: this crust has a thickness ranging from 5 km to 10 km and comprises of denser rocks such as diabase, gabbro and basalt.

2. Continental crust: this crust has a thickness ranging from 30 km to 60 km and comprises of minerals such as quartz, silicate, feldspar, aluminum and less dense rocks such as granite

An igneous rock has a coarse texture and is dark in color. Also, an igneous rock can be accurately described as being produced from magma and formed at Earth’s surface.

Answer:

Produced from magma

Explanation:

I got it right on the test hope this helps

☺️

How does a food chain differ from a food web?
A. A food chain identifies the consumers and producers, while a food web lists all of the animals in an ecosystem
B. A food web shows what to eat, while a food chain shows how the eating patterns are connected.
C. A food chain shows an eating pattern, while a food web shows how the various eating patterns are connected.
D. a food chain shows how eating patterns are connected among organisms, while a food web shows the eating patterns among organisms

Answers

It’s C
Food chains are pretty simple while a food web is very complex

Answer:

C

Explanation:

A food chain shows an eating pattern, while a food web shows how the various eating patterns are connected.

the dna structures ​

Answers

Answer:

Each DNA strand is composed of nucleotides—units made up of a sugar (deoxyribose), a phosphate group, and a nitrogenous base. Each strand of DNA is a polynucleotide composed of units called nucleotides. A nucleotide has three components: a sugar molecule, a phosphate group, and a nitrogenous base. Explanation:

Answer:

are you c a biologist or what

Explanation:

why do you want this answer

What unit of time on Earth is based on the revolution of the Moon around the Earth?
A. day
B. year
C. hour
D. month

Answers

Answer: I think it’s months

Because I found this information... The Moon appears to move completely around the celestial sphere once in about 27.3 days as observed from the Earth. This is called a sidereal month

Explain how an abiotic factor (stronger storms, warmer oceans, or lower ocean pH) can affect populations in a coral reef ecosystem

Answers

Answer:

Climate change leads to: A warming ocean: causes thermal stress that contributes to coral bleaching and infectious disease.

Explanation:

Sea level rise: may lead to increases in sedimentation for reefs located near land-based sources of sediment. Sedimentation runoff can lead to the smothering of coral.

Which of the following does not provide new genetic combinations?

Answers

Can you be more jsnsnsnsnvsvzgs

how do genes create traits in a organism

Answers

Answer:

Like chromosomes, genes also come in pairs. Each of your parents has two copies of each of their genes, and each parent passes along just one copy to make up the genes you have. Genes that are passed on to you determine many of your traits, such as your hair color and skin color.

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HELP!!!!!!!!!
Which kind of molecule provides building blocks for tissues, transports
other molecules, and helps regulate certain reactions in the human body?
A. carbohydrates
B.lipids
C. proteins
D. nucleic acids

Answers

Answer:

C : Proteins

Explanation:

(Can I get brainlest?)

Proteins are the molecules that provide building blocks for tissues, transports other molecules, and helps regulate certain reactions in the human body. The correct option is C.

What are proteins?

A protein is a found naturally, extremely complex substance composed of amino acid residues linked together by peptide bonds.

Proteins are found in all living life forms and contain a variety of important biological compounds such as enzymes, hormones, and antibodies.

Proteins are massive complex molecules that perform numerous important functions in the body.

They are essential for the structure, function, and regulation of the body's tissues and organs and do the majority of the work in cells.

Proteins are molecules that serve as foundation for tissues, transport other molecules, and aid in the regulation of certain bodily reactions.

Thus, the correct option is C.

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Which of the following would be affected when a protein is denatured?
a.) amino groups
b.) primary structure
c.) side chains
d.) tertiary structure

Answers

"b" why because primary structure needs protein and if protein denatured,primary structure will affected instantly!

#followandheartallmyquestion

When a protein is denatured by chemical, heat or other substances, the primary structure remains intact. Hence, the correct answer would be the tertiary structure.

Denaturation of proteins generally causes the breakage of weak bonds on which the folding and the entire protein structure is built. When this happens:

the peptide bonds between the component amino acids remain intact. Hence, the primary structure is conservedthe destruction of other bonds means that the secondary and tertiary structures are affected.

Agents that are capable of denaturing proteins include high temperature, changes in acidity/basicity, UV radiation, high concentration of salts, and so on.

The correct answer as to which would be affected when a protein is denatured in this case would, therefore, be the tertiary structure.

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Break question


what pets would you want




(p.s. could you please answer one of my questions)​

Answers

mmm I really want a dog! thank u for this!
i really want a cat and i might get one

6. If a DNA sequence reads TCCA, a deletion mutation would look like...
a. TCTA
b. TCA
C. TCCCA
d. UCCA
I
I

Answers

Answer: C

Explanation: It would be C because a deletion mutation involves the loss of genetic material, therefore C is the only option that fits the definition.

Hope this helps

Plant growth is the result of cells dividing during _____.
plss help

Answers

Answer:

Plant growth is the result of cells dividing during mitosis.

Explanation:

Mitosis is the process in which cells divide for cell growth or repair. All the cells, as a result of mitosis will have the same number of chromosomes as of the cell at the start.

Answer:

mitosis

Explanation:

Hope this helps

Which statement best explains the ideas of phyletic gradualism and punctuated equilibrium?

A. Phyletic gradualism isn't supported by the fossil record, but punctuated equilibrium is
B. Phyletic gradualism states that many species evolve rapidly at specific points in time, and punctuated equilibrium states that speciation occurs constantly over time
C. Phyletic gradualism states that speciation occurs constantly over time, and punctuated equilibrium states that many species evolve rapidly at specific points in time
D. Phyletic gradualism is supported by the fossil record, but punctuated equilibrium isn't

Answers

A is the correct answer

A. Phyletic gradualism isn't supported by the fossil record, but punctuated equilibrium is

Punctuated equilibrium and phyletic gradualism are two opposing evolutionary tendencies that can be seen in the geological record. Species in punctuated equilibrium tend to demonstrate morphological stasis between rapid speciation episodes, whereas species in phyletic gradualism change more frequently. "Phyletic gradualism isn't supported by the fossil record, but punctuated equilibrium is," says the statement that best summarizes the ideas of phyletic gradualism and punctuated equilibrium.

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which of the following does not affect the rate of photosynthesis a) temperature b) light intensity c) soil d ) water intake

Answers

Answer:

a) temperature

Explanation:

took test and got right

light intensity does not affect the rate of photosynthesis .

what is photosynthesis ?

The process in which plants, algae and certain bacteria use light energy of sunlight, water, carbon dioxide and convert light them into chemical energy and starch. called as photosynthesis.

Photosynthesis occur in special organelles named chloroplast which has its own DNA, genes and hence can synthesize their own proteins.

Chloroplasts have stroma, fluid, and stack of thylakoids known as grana, three types of chlorophyll such as chlorophyll a, chlorophyll b, and carotenoids.

Oxygenic photosynthesis is  commonly observed in plants, algae and cyanobacteria where electrons are transferred from water to carbon dioxide by using light energy, to produce energy,  oxygen is produced along with carbohydrates.

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Which step is a protein formed during gene expression?
a. reverse transcription
b. translation
c. dehydration synthesis
d. DNA replication

Answers

D. DNA replication

DNA always forms proteins

what is a example of acceleration?

Answers

Answer:

For example, if a car turns a corner at a constant speed, it is accelerating because its direction is changing. The quicker you turn, the greater the acceleration. So there is an acceleration when velocity changes either in magnitude or in direction, or both.

Explanation:

hope this helps... (i can't see the pic...)

(─‿─)

HELP PLS!!! (PSYCHOLOGY) I'LL GIVE YOU BRAINLIEST IF UR RIGHT!!

Nate is a teacher who has been in the dassroom for 25 years. When a new teacher is hired, he offers to be a mentor because he loves helping new teachers get inspired about working with children. He wants to help make sure that the teaching profession remains high quality. How would Erikson MOST likely describe Nate's efforts?
A.) Nate is working en generativity.
B.) Nate is working on ego integrity.
C.) Nate is working on intimacy.
D). Nate is working on acceptance​

Answers

Answer:

A.) Nate is working on generativity

Explanation:

Generativity is the ability to provide care or concern for younger or older genrations. Nate offers to be the mentor because he loves helping the new teachers get inspired about working with children. According to Erik Erikson theory of psychosocial development, generativity can refer to "making your mark" or impact on the world through creating or nurturing things that will outlast in an individual/individuals.

Why can grasses not survive on the island yet?​

Answers

why can grasses not survive on what island?
Because it’s dry? I don’t know but I think it’s common sense

What are the nervous and endocrine systems controlling to maintain homeostasis?
O A. the body's nitrogen levels
B. the body's internal environment
OCthe body's external skin and hair
0
D. the body's support for movement

Answers

Answer:

B. the body's internal environment

Explanation:

Homeostasis is defined as the process of maintaining the internal environment of the body.

All the systems work together to maintain homeostasis. The nervous and endocrine systems are two important system that helps in maintaining homeostasis.

The nervous and endocrine systems are linked with hypothalamus that controls the internal environment of the body by releasing and inhibiting hormones. These hormones keep pH,  temperature,  and other internal conditions stable that support homeostasis.

Hence, the correct answer is "B. the body's internal environment".

The hormone insulin is secreted by the pancreas. Which activity is MOST likely to
trigger an increase in the levels of insulin circulating in the blood?
O A. driving
B. eating
O C. jogging
OD. sleeping

Answers

Answer:

Eating

Hope this helps! <3

I will mark brainiest for correct answer

A pelican has a dip netting beak. How does it eat?

A. It traps fish between its upper and lower mandible much like a scissor,
B. It takes a big gulp of water with fish and filters the water off of the fish before swallowing,
C. It opens holes in trees and eats insects beneath the surface of the wood,
D. It burrows in the mud to find insects and other small invertebrates beneath the surface

Answers

Answer:

b is correct

Explanation:

Answer:

b they scoop them and then drain the water a swallow all remaining animals

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Name a place that's in San Juan County. Given the system of equations, what is the value of the system determinant?2x + y = 8x - y = 100-10-20-3 Which of these elements has the lowest ionization energy?A. SiB. CIC. SD. Na Find the equation of the line.Use exact numbers. Brianna has 6 bags of soil. Filling one flowerpot requires 1/3 of a bag of soil. How many flowerpots can be filled with these 6 bags?A.1/2B.2C.18D.24Help I suck at math 0-0 On the map, which colony is the arrow pointing to? (3 points)Thirteen ColoniesAtlantic OceanConnecticutNew JerseyNew HampshireRhode Island Caroline owns 3 fewer T shirts than chase. Let k Represent the number of chases T shirt. What expression can be used? A. 3k B. 3-k C. K+3 D. K-3 Real quick please! PLEASSEEE HELP ASAP THANKS 6. Petra visited the Empire State Building, which is approximately 1454 ft tall. Thescale of the model she bought is 1 in = 200 ft. What is the height of the model ininches? An aphasia that is characterized by fluent speech with good intonation and syntax, but is often semantically incoherent is most commonly associated with: Group of answer choices a. left hemisphere posterior temporal lobe damage b. left hemisphere occipital lobe damage c. a sectioned corpus callosum d. left hemisphere frontal lobe damage Touch the stigma and notice how it feels. What is the advantage of this type of texture? A. The sticky texture allows for capture of prey to help with fertilizationB. The sticky texture allows for the exchange of carbon dioxide to assist with photosynthesis. C. The sticky texture allows for pollen to adhere to the stigma for fertilization. D. The sticky texture allows for water molecules to not escape from the plant, thus preventing dehydration. Based on Benito Mussolini's political philosophy, which of the following policies do you think he would have supported after taking control of the Italian government?A. Promoting equal rights for men and womenB. Extending the right to vote to all citizensC. Increasing funding for the militaryplz answer quick!!!!!!!! Please help due today right now Help and show work please Choose the word that means most important.A.extremeB.familiarC.primaryD.serious Why did Hitler make the move to arrest the leadership of the SA at the Weisee conference? Does the table represent an exponential function? Explain why or why not.(3,-10 ), (4,-7 ), (5,-4), (6,-1), (7,2),(8,5) Is c 9/10 ? Im probably wrong Im a little confused please help 4 Hydrogen has three naturally occurring isotopes. H.1, H-2, and H-3 The atomie mass of Hydrogen is 1.097 Which isotope is most abundant in nature? Explain How can citizens be heroes for the environment?