To assist in completing this question, you may reference the Animated Technique Video - Gel-Filtration Chromatography. Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column

Answers

Answer 1

Answer:

IgG molecule, p53 molecule, actin molecule, histone molecule

Explanation:

In a Gel-Filtration chromatography, the larger molecules elude faster from the gel than the small ones.

In a Gel-Filtration chromatography, there is a stationary phase and a mobile phase. The stationary phase has beads with pores. Small molecules enter the beads and descend through these beads' pores. On the other hand, large molecules do not enter the beads' pores, so they go down through the mobile phase faster than the small molecules. Since they do not have the obstacle of the pores, they descend between the beads more fluidly. As a result, the larger molecules elute first from the gel than the small molecules.


Related Questions

what two things can happen to the food made by plants?​

Answers

Answer:

Food made by plants can get used by plants or by other animals as plants maybe eaten by other organisms that feed on plants by living on them, these organisms are called parasites. Or else the food can be used up by plants to carry out their metabolic activities ( activities at cellular level which keeps the plant alive ) like cellular respiration

Answer:

The food that is prepared by the plant is utilized for its metabolic processes, the excess food is stored in the form of starch, in the plants. ... The plants prepare starch by a process of photosynthesis with the sunlight along with "water and carbon dioxide

The food produced by the plants is in the form of sugar and the extra sugar produced is changed to starch and is stored in different parts of the plant such as in roots, stems, leaves, fruits and flowers.

Explanation:

hope this is what u want

hope it helps u

- Which occurrence is the result of the cell cycle in unicellular organisms?
A. cell repair
B. cell growth
C. cell replacement
D. cell reproduction

Answers

Answer:

D

Explanation:

Cell reproduction is the result of the cell cycle in unicellular organisms. The correct option is D.

What is cell reproduction?

Cells split to produce multiple cells with similar, if not identical, contents. This process is known as cellular reproduction.

Nuclear division during mitosis produces two somatic cells that are genetically identical to the parent cell and have the same genetic complement.

Asexual reproduction in prokaryotes takes the form of cell division in unicellular organisms.

When an organism splits in half, it creates two totally distinct daughter cells, a process known as binary fission. The daughter cells are identical to the parent cells genetically.

Most of the time, Paramecium reproduces asexually via budding, however in poor circumstances, they produce sexually.

Unicellular organisms have a variety of asexual reproduction strategies, including multiple fission, fragmentation, and budding.

Thus, the correct option is D.

For more details regarding cellular reproduction, visit:

https://brainly.com/question/30203012

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lest 5 Adaptation to oxygen up take for mounting climbers​

Answers

increased lung volume,increased lung volume, the ability to carry more oxygen on each red blood cell, increased lung volume, the ability to carry more oxygen on each red blood cell, increased blood vessel diameter increased lung volume, the ability to carry more oxygen on each red blood cell, increased blood vessel diameter faster and deeper breathingincreased lung volume, the ability to carry more oxygen on each red blood cell, increased blood vessel diameter faster and deeper breathingHigh altitude

is the above cell a plant cell or animal cell? provide two observable​

Answers

Explanation:

you have not showed the cell..please

Which of the following are characteristics of Ascomycota? Check all that appply. PLEASE HELP!!

Answers

Answer:

2.spores produced in the ascus

4.important in the food industry

6.can cause disease in plants

Explanation:

Answer:

spores produced in the ascus

important in the food industry

important in the digestion of animals (leafcutter ants for one)

can cause disease in plants (dutch elm disease)

can cause disease in animals (candidiasis for one)

Explanation:

how does shell color work to protect the egg from sunlight?

Answers

Answer:

Birds that live in cool climates lay darker eggs, as darker colors absorb more heat from the sunlight

Birds that live in hotter climates lay lighter eggs to prevent the egg from overheating.

The pigmentation acts to thermo-regulate the internal temperature of the egg, acting as a buffer between the embryo and the outside world. It does this by preventing overheating of the egg, through reflecting the sunlight off the pigmentation, rather than allowing it to be absorbed through the shell

Many of the DD-amino acids found in nature have been discovered in bacterially produced peptides that have antibiotic properties.

a. True
b. False

Answers

Answer:

True

Explanation:

Most the amino acid are of L configuration. Antimicrobial peptides consists of D amino acids (D amino acid occur naturally). D amino acids are also found in walls of microorganism. Antimicrobial peptides is released by species including bacteria, fungi etc.

For example - Bacteriocin, peptaibols, plectasin etc.

Hence the given statement is true

In which stage of the cell cycle does the cell die
A) interphase
B) mitosis
C) apoptosis

Answers

The short answer is that cell death by apoptosis in various phases of cell cycle depends on the cell-type and inducer/stimulus. Hematopoietic cells can undergo apoptosis at all stages of cell cycle as noted by Bertrand. However, mesenchymal or epithelial cells usually undergo apoptosis at the G1/S or G2/M phase of the cell cycle.

For tetracycline , describe an experiment that you could
use to determine if a bacterial sample taken from a person was
resistant to your antibiotic. Be sure to describe all steps of the
experiment including your independent, dependent and control
variables.

Answers

Answer:

penicillin was the first antibiotic discovered. Alexander Fleming discovered it in 1928. It was used widely during World War II. However, the sulfonamides were the first antibiotic class used clinically (in the 1930's). The discovery of antibiotics revolutionized medicine by making once-deadly infections treatable. There are now hundreds of antibiotics, classified into several categories. These antibiotic classes include:

Explanation:

sana makatulong!

Which characteristic helps grasses survive dry conditions?
long, flexible stems
long, skinny leaves
small, soft flowers
deep roots

Answers

answer: deep roots.              hop u do good on ur test guys

a radioactive element has a half-life of 3,000 years. if a sample of this element begins with a mass of 60 grams, how long would you have to wait for the mass to decrease to 15 grams

Answers

Answer:

N = 60 g

Half life : N/2 = 3000

N/4 = 15 g

where is the most energy stored in the ATP molecule stored

Answers

Energy is stored in the covalent bonds between phosphates

What do producers dictate in
an ecosystem?
A. the size of the organisms
B. the age of the organisms
C. the amount of organisms

Answers

Answer:

C. The amount of organisms

Explanation:

Producers produce food from inorganic matter, this then makes food for other organisms in an ecosystem. The amount of producers dictates how much food is available, and by that how many organisms can be present.

The answer is C hope this helps

12. Removing an organism from an ecosystem will have which of the following affects? Select all that apply.
A. Increase in biodiversity
B. Decrease in biodiversity
C. Increase in the overall health of the ecosystem
D. Decrease in the overall health of the ecosystem​

Answers

Answer:

B and D

Explanation:

Biodiversity comes from having multiple types of organisms. This also allows different organisms to eat and create nutrients that is needed by all other organisms in the ecosystem.

The prolonged increase in heart and breathing rates during the snow skiing trip was probably a result of:______

a. activation of the sympathetic autonomic nervous system by the new experience.
b. activation of the parasympathetic autonomic nervous system by the new experience.
c. hypoxia caused by insufficient blood hemoglobin concentration to supply oxygen for exercise at the low oxygen pressure found at high altitudes.
d. depressed core body temperature (hypothermia) caused by exposure to cold temperatures at high altitudes.

Answers

Answer:

The correct answer is - option C.

Explanation:

Skiing generally happens on higher altitudes such as mountains. It is known that there is less oxygen is available at higher altitudes. There is low oxygen pressure at such height.

The prolonged increase in heart and breathing rate is due to less oxygen available for the cells. This condition is known as hypoxia caused by insufficient blood hemoglobin to supply oxygen for physical activities at higher altitudes.

the average weather pattern taken over a long period of time is called ............. of that place.

Answers

Answer:

Climate

Explanation:

Comment on the folding of protein in vaccum​

Answers

Answer:

Protein folding is a process by which a polypeptide chain folds to become a biologically active protein in its native 3D structure. ... The amino acids in the chain eventually interact with each other to form a well-defined, folded protein. The amino acid sequence of a protein determines its 3D structure.

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Allergies, asthma, high blood pressure, and heart
disease are examples of conditions caused by

Answers

Answer:

pollen sorry

Explanation:

i only did this for the points but the real answer is:

Children with allergies, asthma may be at risk of heart problems, with an increased risk of developing high blood pressure, high cholesterol, and obesity. In addition, eczema, a condition that causes itchy, inflamed skin, was common to asthma. here you go! Have a great day!

What is your opinion about minor children (under the age of 18) donating one of their kidneys for their brother or sister? Write at least three sentences stating your opinion and backing your answer.

Answers

i believe that children under the age of 18 should not be permitted to donate kidneys. it could possibly cause health issues down the line. also decisions made at a young age might not be a good idea.

How are gymnosperm seeds and angiosperm seeds different?

Answers

Answer:

angiosperms have seeds that are closed within an ovary, gymnosperms have no flowers or fruits, and have unenclosed seeds on the surface of scales or leaves

A)x-linked
B)No answer text provided
C)recessive
D)dominant

Answers

Answer:

D)dominant

Explanation:

The correct answer is D) dominant

The Earth is the only planet known to support life. Which of the following is a major contributing factor to the Earth's ability to support
life?

A) The Earth has a magnetic field with north and south poles

B) The earth has an iron-rich core and is made mostly of rock

C) The Earth orbits the Sun and is orbited by the moon

D) The Earth is surrounded by a layer of air that includes oxygen

Answers

Answer:) The Earth has a magnetic field with north and south poles

Explanation:

Soils in different regions differ based on the _______.
a.
local climate
b.
topography of the land
c.
native organisms
d.
all of the above

Answers

Answer:

I think the answer should be local climate

Answer:

i think it's D.

Explanation:

because i just want to do an anser on this thing

What happens after Telophase?
&
36/86
>
C

Answers

Answer:

The chromosomes begin to uncoil, which makes them diffuse and less compact. Along with telophase, the cell undergoes a process called cytokinesis that divides the cytoplasm of the parental cell into two daughter cells.

what is Otis’s gene versions for body color

Answers

Answer:

giao

Explanation:

Why is a pasture-type system is
more labor intensive than
controlled systems?
A. There is greater maintenance of the
equipment.
B. There is greater need to handle diseases.
C. There is greater area to cover to observe and
manage the animals.
D. There is greater cost of feed per animal.

Answers

Answer:

The correct option is C.

There is greater area to cover to observe and

manage the animals.

Explanation:

This is because pasture type system refers to a large area of land where grasses are grown and animals are allow to graze the area of land to obtained nutrients. In this system there is large area of land where animals are well observed while feeding in order to prevent the risk of loss and attacks.

_____ feedback is when the body reacts in the same direction as a stimulus.

Negative
Positive

Answers

Answer:

It's Positive.

Negative is when it reacts against the direction as a stimulus  

the answer is positive

The temperature at which air becomes saturated with water vapor is called the _____.

Answers

Answer:

dew point

Explanation:

if an earthworm is 18mm long and is photographed and the picture is magnified 2.5x how long will it be in the picture?

Answers

Answer:

The earthworm will be 45 mm long in the picture

Explanation:

From the problem, we can take the original size of the earthworm to be 18mm.

Magnification  can be obtained using the formula:

magnification = observed size/ original size.

Recall, we are given that the magnification is 2.5.

Hence, can evaluate the size in the picture to be

2.5 X 18mm = 45mm

With the help of circulatory it produce blood cells.


A. Excretory
B. Muscular
C. Endocrine
D. Skeletal

Answers

Answer:

Skeletal

Explanation:

Think about the bone marrow.

Answer:

D. Skeletal

Explanation:

blood cells are formed in the red bone marrow of bones. Stem cells in the red bone marrow are called hemocytoblasts. They give rise to all of the formed elements in blood. If a stem cell commits to becoming a cell called a proerythroblast, it will develop into a new red blood cell.

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