The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 4 repeats in your product, what was the size of your final PCR product?

186

214

158

200

172

Answers

Answer 1

Answer:

186

Explanation:

The Polymerase Chain Reaction (PCR) is a technique used in molecular biology to amplify small segments of DNA. This technique uses a pair of oligonucleotide sequences called 'primers' which bind to opposite strands of the heat-denatured DNA template in order to amplify this target DNA sequence. In this case, the DNA template is composed of a single unit of 130 base pairs (bp) and four repetitive units of 14 bp, so the final PCR product will have 186 bp >>> 130  bp + (14 bp x 4) = 130 + 56 = 186.


Related Questions

7. During DNA replication, a complementary strand of DNA is made for each original DNA strand. Thus,
if a portion of the original strand is CAGGTTACG, then the new strand will be
a. CAGGTTACG.
c. GTCCAATGC.
b. GTCCTTAGC.
d. GTCCTTACG.

Answers

Answer:

i think its b i took the test but i forgot the answer

Explanation:

HELP!!
A mother with the genotype AA displays the dominant version of the trait. Her husband displays the recessive version of the trait. Their child displays the dominant version of the trait. How is this possible?


A. The mother gave a recessive allele to their child.


B. The father gave two recessive alleles to their child.


C. The mother gave the dominant allele to their child.


D. The father gave the dominant allele to their child

Answers

Answer: C. The mother gave the dominant allele to their child.

Which part of the female reproductive system is highlighted below?
A. Fallopian tube
B. Ovary
C. Uterus
D. Cervix

Answers

Answer:

C. Uterus

Explanation:

Uterus is the female reproductive system.

What is Female reproductive system?

The female reproductive system serves a number of purposes. It aids in sexual reproduction in addition to enabling sexual activity.

You make eggs using your ovaries. During ovulation, these eggs are subsequently moved to your fallopian tube where sperm fertilization may take place. The fertilized egg is then transferred to your uterus, where the lining of the uterus has expanded due to the typical hormones produced during your menstrual cycle, also known as your reproductive cycle.

The fertilized egg can implant into the thicker uterine lining once it's within your uterus and continue to grow there. Your menstrual period causes the uterine lining to shed if implantation doesn't occur.

Therefore, Uterus is the female reproductive system.

To learn more about Uterus, refer to the link:

https://brainly.com/question/9778292

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Compared to freshwater fishes, marine fishes: drink seawater and produce a large volume of urine. do not drink seawater in an effort to conserve as much water as possible. produce a large volume of dilute urine in an effort to rid their bodies of excess water. tend to gain water by osmosis since their internal salt concentration is higher than that of seawater. tend to lose water by osmosis since their internal salt concentration is lower than that of seawater.

Answers

Answer:

The correct answer is - tend to lose water by osmosis since their internal salt concentration is lower than that of seawater.

Explanation:

In freshwater fishes, the body of fishes has a higher salt concentration inside their body than the surrounding water, water enters through the osmosis process. Without any active regulation of this process, fishes would swell and get bigger and bigger. They have specialized cells called chloride cells in gills to take ions from water as they do not have kidneys.

In contrast, the marine fishes have a lower salt concentration in their body than surrounding water of sea or ocean and they lose water continuously and to compensate for this they need to drink water regularly.

Does anyone know why Mt. Vesuvius erupted? Was it natural or human caused?

Answers

Answer:

Explanation:

Natural

An individual who is establishing a strategy to use in order to accomplish a goal is demonstrating which phase of the self-regulation
cycle?

O self-reflection

O performance

O forethought

O goal accomplishment

Answers

Answer:

Forethought

Explanation: Basically means planning ahead of time in order to know what to do when you are faced in a situation or event that will happen in the future

Answer:

The answer is C. Forethought

Explanation:

I Now Give Up On Life :) Don't Try And Stop Me

Answers

Wdym you give up...

hii, NEVER GIVE UP. <3

What is the final result of genetic drift in populations of

Answers

Answer: Genetic drift may result in the loss of some alleles (including beneficial ones) and the fixation, or rise to 100% frequency, of other alleles.Once it begins, genetic drift will continue until the involved allele is either lost by a population or is the only allele present at a particular gene locus within a population. ... Genetic drift can result in the loss of rare alleles, and can decrease the size of the gene pool.

Explanation:

Answer:

decrease the genetic diversity of a population.

Explanation:

Genetic drift describes random fluctuations in the numbers of gene variants in a population. Genetic drift takes place when the occurrence of variant forms of a gene, called alleles, increases and decreases by chance over time.

Genetic drift can cause a new population to be genetically distinct from its original population, which has led to the hypothesis that genetic drift plays a role in the evolution of new species.

Which of the following steps stops the feedback
mechanism that begins when a person's body
temperature falls below 37.C?
O A. The blood vessels slow blood flow to the
skin.
B. The brain senses that the body temperature
has reached 37°C.
C. The muscles begin to contract rapidly.
O D. The person begins to sweat.

Answers

Answer:

dont know sry

Explanation:

??????????????

Answer: B

Explanation: The brain senses that the body temperature has reached 37°C

Summer happens when the Earth is closest to the Sun.
True or False

!!!HELP QUICK!!!

Answers

Answer: False

Explanation:

Summer happens when the Earth is far away from the Sum

true, earth gets closer to the sun so it gets hot

Why should the general public be concerned about the survival of honeybees

Answers

Answer: honeybees are one of the most important pollinators in the world, we do have others but without bees many crops, and plants will die and that will result in animals dying.

Explanation:

Because the honeybees are

Any change in the Relative Frequency of alleles in a population is:

Controlled by more than 2 genes

Controlled by a single gene

The gene pool

Evolution

Answers

It’s hard brother! Pool

With respect to climate change and natural selection acting upon a population of one species of frog in a tropical rain forest, mutations of DNA sequences of the frog genome that are inherited by offspring It is estimated by a wide body of scientists that in recent decades we have begun another mass extinction event on Earth. Amphibians in particular have been in decline, sometimes due to climate change, and they therefore provide a possible early sign of dramatic biodiversity change to come. With respect to climate change and natural selection acting upon a population of one species of frog in a tropical rain forest, mutations of DNA sequences of the frog genome that are inherited by offspring may have no effect at all, be beneficial, or be harmful. will primarily be harmful. will only speed the decline and extinction of the species. could either be beneficial or harmful.

Answers

Answer:

be beneficial.

Explanation:

Mutations of DNA sequences of the frog genome that are inherited by offspring will be beneficial for the frog because this mutation in the genetic makeup of frog provides resistance in this new environmental conditions. This mutation occurs due to the exposure of cell's DNA to the environmental factor which gives benefit to the organisms to survive different condition which can't tolerate by their parents so this mutation is beneficial for that offspring of frog.

The most likely reason(s) a metastatic tumor cell might lose its connection to the basal lamina would be: a change in the integrin proteins from the cell's surface. a change in the cadherin proteins on the cell surface. a change in connexins disrupting gap junctions. All of these choices are correct.

Answers

Answer:

A change in the integrin proteins from the cell's surface.

Explanation:

Integrin proteins' principal function is to attach the cell to the basal lamina. Also, they transmit signals from the extracellular matrix to the interior of the cell. A change in the integrin could result in loss of connection between the mutated cell and the basal lamina.

Cadherin proteins connect one cell to another. A change in these proteins will not result in the loss of connection between the metastatic tumor cell and its extracellular matrix. It most likely will affect the communication and adhesion of cells.

A change in connexins will disrupt the communication between cells but not the connection to the lamina basal. Connexins are transmembrane proteins that connect one cell to another with gap junctions that allow the passage of different components from cell to cell.

True or false
Prokaryotic cells can’t replicate DNA, because they do NOT possess nuclei.

Answers

Answer:

False

Explanation:

Prokaryotes do have their genomic DNA concentrated and localized to a small area within the cell (nucleoid region). So it's not entirely accurate to say that prokaryotes don't have a nucleus.

Answer:

true

Explanation:

prokaryotics are cells without nucleus

Which best describes a method of obtaining energy that may cause changes in water temperature and harm to fish populations?

Answers

generating electricity in a hydroelectric power plant.

3. Please rank the following topics from most to least interesting: (Fire Ecology - (FE), Noise Pollution and Mitigation Strategies (NP), College Majors and Careers in the Environment (CC), Great Pacific Garbage Patch (GP), EcoTourism

1.
2.
3.
4.
5.
6. ​

Answers

1.FE
2.NP
3.CC
4.EcoTourism
5.GP

What is the difference between a) prokaryote and eukaryote; b) autotroph and heterotroph; c) unicellular and multicellular?

Answers

Answer:

Explanation:

a) eukaryotes have membrane bound organelles like nuclei while prokaryotes don't

b) autotrophs = producers that can make their own food while heterotrophs consume producers or other consumers

c) Unicellular organisms are made up of only one cell that carries out all of the functions needed by the organism, while multicellular organisms use many different cells to function

What are exons and introns and how are they dealt with in the cell?

Answers

Answer:

Exons are coding sections of an RNA transcript, or the DNA encoding it, that are translated into protein. Exons can be separated by intervening sections of DNA that do not code for proteins, known as introns.

Explanation:

The population of peppered moths have changed over time. The population has changed from primarily light colored to dark colored moths. This is an example of...



a
Mimicry
b
Natural Selection
c
Selective Breeding
d
Camouflage

Answers

Answer: b

Explanation:

natural selection occurs when something adapted to the environment to give them an advantage or to survive  

. ___________________ is the process of plants releasing oxygen into the environment?

Answers

Answer:

photosynthesis

Explanation:

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What property of silver may discourage people from buying silver jewelry?

A. it can’t float
B. it’s not green
C. it can tarnish
D. it’s not transparent

Answers

Answer: C

Explanation: Because if silver is tarnished it will lose its luster, thus making it not attractive.

C! Hope this helped!

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Name the types of adaptation that animals and plants can have

Answers

Answer:

poison, thorns, sharp teeth

Explanation:

Unicellular organisms are able to survive on their own because they have structures that have what important characteristic?

Answers

Answer:

A unicellular organisms depends upon its one cell for all the functions and survival of the organism. It has all the important characteristics that is necessary for its survival.

Explanation:

A unicellular organism is one which contains only one cell. And so all the life processes occurs in that one particular cell in an unicellular organism. A n unicellular organism is also called as the single cell organism.

The unicellular organisms uses cilia as well as the flagella to achieve locomotion by creating currents around and move about places. The take up nutrients by diffusion process. They reproduce asexually and produce their offspring.

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Margo is interested in pursuing a degree in agriculture. To decide on a career field, what should she consider first?


desired working conditions and lifestyle

history of agriculture and benefits

types of livestock and crop production

innovations in farming and necessary education

Answers

Answer:

I would say desired working conditions and lifestyle, since everything you do would revolve around that. I'm not sure if it's correct though.

Explanation:

Answer:

C.

Explanation:

can someone please help me

Answers

I’m not sure how I can help you with that
Same don’t know how I can help

A scientist studying the effects of radiation on DNA performs an experiment in which she exposes a sequence of nitrogen bases found in DNA to radiation. The DNA before and after the radiation is shown below.
Use the evidence to determine whether a mutation has occurred, and if so, interpret that mutation in the answer sheet provided

Before Exposure: ATCGTCA; After Exposure: ATTCGTCA

A. No mutation has occurred to the DNA sequence.

B. A nitrogen base has been deleted from the DNA molecule.

C. A nitrogen base has replaced another nitrogen base in the DNA molecule.

D. A nitrogen base has been added to the DNA molecule.

Answers

Answer:

D. A nitrogen base has been added to the DNA molecule.  

Explanation:

You can see that the sequence for the nitrogen bases are in the exact same order after the exposure, this rules out A.  You can count how many bases there are, 7 before and 8 after so it cannot be B. Lastly, given that they are in the same order as were before and that a base has been added, you can see that no bases were replaced (so not C) and that D is your answer.  

When a plant doesn't have enough water, it will wilt and _____ .
A) the amount of carbon dioxide will increase
B) the amount of carbon dioxide will decrease.
C) the amount of oxygen will increase.
D) the rate of photosynthesis will increase

Answers

Answer:

The amount of Carbon Dioxide will increase.

Explanation:

Answer:

When a plant doesn't have enough water, it will wilt and the amount of carbon dioxide will decrease.

Explanation:

You're welcome!

A wealthy elderly couple dies together in an accident. A man comes forward, claiming that he is their long lost son and is entitled to their fortune. The couple were of blood types AB and O. The man has type O blood. Could he be the heir to the fortune?
Group of answer choices

No, you cannot get type O from crossing type AB with type O

Yes, you can get type O from crossing type AB with type O

Answers

The answer is NO

An O parent and an AB parent can only make type A or type B it is not possible to get type O in this scenario. Hope this helps

Choose the best answer.
Crossing over is important because it creates

A)gene splicing
B)new breed
C)genetic diversity
D)zygote
E)double helix

Answers

Answer: C) genetic diversity

Explainiton : Crossing over is essential for the normal segregation of chromosomes during meiosis. Crossing over also accounts for genetic variation, because due to the swapping of genetic material during crossing over, the chromatids held together by the centromere are no longer identical.
C- Genetic diversity !
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