The owners of a local bakery ask for your help in improving a special yeast strain they use to make bread. They would like you to help them design experiments using RNA interference to turn off genes, to allow them to test their hypothesis that certain genes are important for the good flavors found in their bread. Of the components in the following list, which is the most important to check for in this yeast strain if you would like this project to succeed?

a. the presence of foreign double-stranded RNA
b. the presence of genes in the genome that code for RISC proteins
c. the presence of miRNA genes in the genome
d. the presence of single-stranded siRNAs within the cell

Answers

Answer 1

Answer:

b. the presence of genes in the genome that code for RISC proteins

Explanation:

The RNA interference (RNAi) mechanism is a naturally occurring pathway by which eukaryotic cells can silence specific genes both at the transcriptional (eg., by triggering DNA methylation on specific loci) and posttranscriptional (e.g., by triggering mRNA degradation) levels. This mechanism (RNAi) is widely used in molecular biology laboratories in order to silence genes and thus understand their functions. During the RNAi pathway, double-stranded RNAs (dsRNAs) are cleaved into small double-stranded RNA (dsRNA) molecules known as small interfering RNAs (siRNAs) or microRNAs (miRNAs) by an enzyme referred to as "Dicer". Subsequently, these small non-coding RNAs bind to an enzymatic complex known as RNA-induced silencing complex (RISC) that contains a ribonuclease enzyme, i.e., an enzyme that degrades RNA. Finally, the small non-coding RNA (either siRNAs or miRNAs) that are complementary to the RNA target directs the RISC complex to bind and cleave specific mRNAs, thereby silencing gene expression (in this case, by the inhibition of protein translation).


Related Questions

You identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with Y. Based on this, which enzymes can you say with certainty is/are disrupted by the mutation

Answers

Answer: Enzymes in both Q and Y pathway are disrupted because you have to supplement the medium with those components for the organism to grow.

Explanation:

A microorganism is auxotrophic when it is only able to proliferate in a culture medium if some specific substance has been added to it, which the wild type (prototroph) does not require, because it can synthezise it. The underlying genetics of auxotrophy is the lack of a functional metabolic pathway that generates the substance on which the auxotroph depends. This lack is usually due to a mutation that generates a null allele lacking biological capacity. So, due to a deficiency in an enzyme required for the synthesis of a certain component, the organism requires that component in the culture medium in order to grow.

A metabolic pathway is a set of consecutive chemical reactions catalyzed by enzymes synthesized by the cell, in which the product of one is the substrate of another. In living organisms there are multiple metabolic pathways, responsible for synthesizing and metabolizing compounds (cellular components, regulatory molecules, compounds that serve to store energy), or degrading them to obtain energy. Thus, enzymes catalyze chemical reactions, this means they accelerate the rate of reaction.

A normal medium is one that possesses all the nutrients and compounds necessary for the proper functioning of a normal organism (prototroph). However, if the organism lacks, for example, an enzyme, it will not grow in a normal medium because it does not have the enzyme to make the components. If the organism does not grow in the normal environment, then it has some mutation that causes some enzyme not to be produced. Remember that enzymes are proteins, and all proteins are encoded by DNA, so if there is a mutation in the DNA, in the gene that encodes the enzyme, the enzyme will not be synthesized or will be synthesized but in such a way that it cannot function properly.

If the organisms only grow in medium supplemented with Q and in medium supplemented with Y, then this indicates that it has a defect in one or more enzymes in Q and Y pathway, because the organism cannot produce certain components and they need to be added in the environment. The problem does not provide enough information to know if for each pathway only one enzyme is affected or several, but it is enough to say that enzymes in both Q and Y pathway are disrupted because you have to supplement the medium with those components for the organism to grow.

What are possible effects warming the atmosphere?​

Answers

Higher temperatures are worsening many types of disasters, including storms, heat waves, floods, and droughts. A warmer climate creates an atmosphere that can collect, retain, and drop more water, changing weather patterns in such a way that wet areas become wetter and dry areas drier.

HELP WITH THESE ASAP NO LINKS PLS

Answers

16. should be B) Chemical Properties of Water.

Because those are all examples of chemical changes of water.

17. should be B) The liquid will remain a liquid.

Because 52° C is the same as 125.6° F.

Qué tipos de lípidos forman parte de la membrana celular?

Answers

Answer:

Hay tres clases principales de moléculas de lípidos de membrana: fosfolípidos, colesterol y glicolípidos. Las composiciones de lípidos de las monocapas internas y externas son diferentes, lo que refleja las diferentes funciones de las dos caras de una membrana celular.

Explanation:

Cell phones are widely used by people all over the world as a medium of communication. When a person makes a call from a cell phone, the cell phone emits electromagnetic waves that are intercepted by a nearby cell phone tower. The cell phone tower then connects the call to the phone of the person being called.

Which of the following processes enables a cell phone to receive communications from a cell phone tower?
A.
Electromagnetic waves diffract around the cell phone's antenna, which allows the waves to be detected by the internal circuits of the phone.
B.
Electromagnetic waves from the tower interfere with the radio waves emitted by the cell phone, producing electric currents in the internal circuits of the phone.
C.
Electromagnetic waves from the tower produce an electric current in the cell phone's internal circuit when intercepted by the phone's antenna.
D.
Electromagnetic waves from the tower are reflected by the cell phone, producing an electromagnetic field that is detected by the internal circuits of the phone.
R

Answers

Electromagnetic waves diffract around the cell phones antenna

Answer:

Electromagnetic waves from the tower produce an electric current in the cell phone's internal circuit when intercepted by the phone's antenna.

Explanation:

Study Island Answer

In primary mutant screens, A) mutant flies are identified by morphology and then the mutant genes are isolated and studied. B) mutant flies are created by RNAi using random RNA sequences and then the genes affected identified. C) mutant flies are created by gain-of-function expression of random genes. D) two mutant flies are mated to determine if there is an effect on morphology.

Answers

Answer:

A) mutant flies are identified by morphology and then the mutant genes are isolated and studied.

Explanation:

The visualization of mutant flies is done with an experiment, where drosophila flies, also known as fruit flies, are captured and placed in closed bottles. The bottles have a mixture of banana and agar so that the flies can feed for a period of 15 days. During these 15 days, the flies made several crosses and over time, it will be possible to identify the appearance of mutant flies as a result of the number of crosses made inside the bottle. The mutant flies will be identified when the researcher is able to observe flies with legs, eyes and wings in different numbers than what is presented in a normal fly.

After being identified by their different morphology, the mutant flies will have their mutant genes isolated and studied.

If a drug was administered that would impede the binding of NAD+ to electrons what steps of cellular respiration would this substance affect

Answers

Answer:

The correct answer is - not getting all NAD+ so burning more ATP to make up for the energy loss.

Explanation:

If the drug was administered and it would cause impedetion of the binding of NAD+ to electrons which causes decrease availability of the NAD+ that leads to less energy formation due to the use up more ATPs in the process of cellular respiration.

If NADH couldn't be formed as NAD+ will not binding, the cells will lose out on ATP produced. It will lead to weight loss due to burning out more ATP to make up for the energy loss.

Birds are the main predators of these beetles; they hunt the beetles by sight and pick them off the tree bark where they feed. A logging company clears trees off an island and plants younger trees as a replacement. Trees with light bark are planted on one side of the island, and trees with dark bark are planted on the other side of the island. What do you predict would happen to the range of coloration in the population of beetles living on the light-colored trees of the island

Answers

Complete question:

1. The graph below depicts the frequency of expression of the coloration of beetles on an island:

Graph is the 1st Pic --> Attached files    

Birds are the main predators of these beetles; they hunt the beetles by sight and pick them up off of the tree bark where they feed. A logging company clears the trees off of the island and plants younger trees as a replacement. Trees with light bark are planted on one side of the island and trees with dark bark are planted on the other side of the island. What do you predict would happen to the range of coloration in the populations of beetles on each side of the island?

Select TWO answers, one to represent the beetles living on the light colored trees and one to represent the beetles living on the dark colored trees. (2 points)

A. graph 2 below

B. graph 3 below

C. graph 4 below

D. graph 5 below

Answer:

B. graph 3 below

D. graph 5 below

Explanation:

The original population of beetles (Graph 1) shows that most individuals had an intermediate phenotype between dark and light color. The graph expresses its highest point in the middle of the phenotypic ax, while the curve´s tails coincide with light and dark colors.

Probably, most of the trees inhabiting the whole island had some darkish color tone, which beetles used to camouflage and avoid predation.

When the company cleared the area and reforested, they planted light trees on one half and dark trees on the other half of the island. This change in the environment modeled the beetled phenotype, together with predation pressure.

If we consider the whole original population, we should say that it went under a disruptive selection. But if we think about the sides of the island separately, we could say that the population living on each side went under directional selection. We are not thinking about the whole population of beetled inhabiting the whole island, but two groups inhabiting each side of the island.

Graph 3 represents the beetles inhabiting the part of the land with dark trees. The population went under directional selection, and most of them turned into dark-colored to camouflage with the substrate in which they live.Graph 5 represents the beetles inhabiting the other part of the land with light trees. The population went under directional selection, and most of them became light-colored to camouflage with the substrate in which they live.

Over generations of time, what would you expect a population of organisms that are well adapted to their environment to do?

Increase
Decrease
Become Extinct
Stay the same

Answers

Answer:

Increase.

Explanation:

Because they have to adapt if they don't the will be extinct.

which two statements best describes the position of a coastal area during a high tide?

a. it is parallel to earths axis
b. it faces directly toward or away from the moon
c. it is parallel to earths orbit plane
d. it is perpendicular to the moon

Answers

The two statements that best describes the position of a coastal area during a high tide is that it is perpendicular to the moon which is therefore denoted as option D.

What is a Tide?

This is referred to the are very long-period waves that move through the ocean in response to the forces exerted by the moon and sun an there are two types which are high and low tide.

The position of coastal ares during high tide is that it is perpendicular to the moon which is why the side of the Earth closest to the Moon experiences the Moon's pull the strongest, and this causes the seas to rise, creating high tides.

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Answer:

The answer would be B

Explanation:

Which scientist devised tests that helped confirm that bacteria and other microorganisms cause a variety of diseases?
Robert Koch
Anton van Leeuwenhoek
Louis Pasteur
Alexander Fleming

Answers

Answer:

A. Robert Koch

Explanation:

I got a 100 on the pre-test (Edgen2021)

Robert Koch made tests that helped confirm that bacteria and other microorganisms cause a variety of diseases.

Who was Robert Koch?

Robert Koch was a scientist that work to identify many pathogenic microorganisms (e.g., bacteria and fungi).

Nowadays, Robert Koch is considered the father of modern bacteriology.

Robert Koch identified many pathogenic microorganisms including Mycobacterium tuberculosis (tuberculosis) and Vibrio cholera (cholera).

In conclusion, Robert Koch made tests that helped confirm that bacteria and other microorganisms cause a variety of diseases.

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PLEASE ANSWER ALL

question 21
An animal that only eats the flesh of another animal is called a

A. producer
B. herbivore
C. carnivore
D. omnivore
______________________

question 22
Which of the following statements is true about matter and energy:

A. matter is not recycled, energy is not recycled
B. matter is recycled, energy is not recycled
C. both matter and energy are recycled
D. neither matter nor energy are recycled
______________________

question 23
On what trophic level would you find organisms that use the suns energy directly to make their own food?

A. 1
B. 2
C. 3
D. 4
_______________________

question 24
Which of the following is an abiotic factor in an ecosystem?

A. bird
B. tree
C. rock
D. deer
______________________

question 25
Mushrooms and other fungi are responsible for breaking down dead organic matter. In the ecosystem, they are called:

A. producers
B. comsumers
C. decomposers
D. scavengers

Answers

21: c. Carnivore

22: C. Both matter and energy are recycled (I think)

23: A. 1

24: B. Rock

25: C. Decomposers
Omnivore b. 3. Deer. Consumers


The graphs here show the distribution of beak depths for the ground
finches on Daphne Major, an island in the Galapagos. The bird beaks were
measured over a multi-year study, including a time period before and after
a drought. The drought reduced the supply of seeds and left most of the
seeds as thick-walled nuts that survived the drought. Analyze the data and
select the claim about natural selection and genetic diversity that is
supported by the data.

Natural selection tends to reduce genetic variation, as there was a smaller distribution
of beak depths, but it tends to select for the most fit phenotypes, as there was an
increase in average beak depth after the drought.

Natural selection tends to increase genetic variation, as there were more ground
finches after the drought, and it tends to select for the most fit phenotypes, as there
was an increase in average beak depth after the drought.

Natural selection tends to reduce genetic variation, as there was a smaller distribution
of beak depths, but it tends to select against the most fit phenotypes, as there was an
increase in average beak depth after the drought.

Natural selection tends to increase genetic variation, as the average beak size
increased after the drought, but it tends to select against the most fit phenotypes, as
there was a decrease in population size for the finches after the drought

Answers

Answer: (A) Natural selection tend to reduce genetic variation as there was a smaller distribution of beak depths, but it tends to select for the most fit phenotypes, as there was an increase in average beak depth after the drought.

Explanation: The picture below shows proof if needed. On USATP

Natural selection tend to reduce genetic variation as a smaller allocation of beak depths, but it tends to select for the most fit phenotypes, as an increase in average beak depth after the drought.

How does natural selection affect genetic variation?

Genetic variations that vary gene and protein role.

If a trait is favourable and helps the someone survive and breed, the genetic variation is more likely to be passed to the next generation.

Thus, option "A" is correct by selecting for or against a specific gene.

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Which of these actions would be controlled by the somatic nervous division of the peripheral nervous system?

A. Blood vessel dialation

B. Movement of food down the esophagus

C. Opening and closing of the eyes

D.Opening and closing of the pupils

Answers

Answer:

C

Explanation:

What is the function of the skin in protecting the body from disease?

Answers

Answer:

A.killing the infection

The answer is...

A.killing the infection!

Freshwater ecosystems can be grouped as those that are still, those that are flowing, and those that contain salt.

Answers

Answer:

true

Explanation:

What kingdom is made of organisms that are eukaryotic, plant-like, do not move, and do not carry on photosynthesis?

Answers

Answer:

protists

Explanation:

Answer:

Protists are organisms that are part of the biological kingdom called the protista. These organisms are not plants, animals, bacteria, or fungi. Protists are a very diverse group of organisms. They are basically all the organisms that don't fit into the other groups.

Explanation:

Discus four
importance of applications software​

Answers

Answer:

1.Application software help the end users perform single or multiple tasks.

2.All the useful work are done by application software.

3.It is a word processing software.

4.It control and manage the operations of the computer.

Explanation:

It can be used for general purpose as well as specific function.

If a person is lactose intolerant describe the specific steps that would follow once lactose into the small intestines

Answers

Causes gas or even diarraha if it reaches the intestins

Self-report inventories and projective tests are examples of personality assessments.
Please select the best answer from the choices provided
T
F

Answers

The correct answer is T

It is true that Self-report inventories and projective tests are examples of personality assessments.

What is personality assessment?

Personality Assessment can be define proficiency of assessment which involves how personality traits can be administer, score, interpreted using supportive measures in order to refine structures, psychological interventions and so on.

Therefore, is true that Self-report inventories and projective tests are examples of personality assessments.

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Ángel es un cerdo??
a) Sí
b) Obvio que sí

Answers

OBVIO QUE SI WEY .O SEA ES B!

c)n op orque si es el el uqe no tiene pecho

Which is true about the tundra biome?
a. It receives less direct sunlight than any other biome on earth.
b. It has more trees than any other biome on earth.
c. It can be found at low elevations all over the earth.
d. It has more types of plants and animals than any other biome on earth.

Answers

Answer:

It receives less direct sunlight than any other biome on earth.

Explanation:

The tundra biome is a cold, frozen landscape. This biome has a short growing season, followed by harsh conditions that the plants and animals in the region need special adaptations to survive. Tundra form in two distinct cold and dry regions.

The Tundra biome is a frozen landscape that has a dark and cold environment. It receives less sunlight compared to other biomes. Thus, option a is correct.

What are the characteristics of the Tundra biome?

The Tundra biome is the biogeographical region that has the coldest atmosphere of all the other biomes and is marked by little precipitation, low temperatures, long winters, and short seasons for agricultural growth.

They are present in the Arctic circle as the temperature reaches below zero degrees celsius which can last up to ten months. It is mostly barren land and is majorly covered with willow trees, and birch trees. They receive very less amount of sunlight as they have a long winter season.

Therefore, Tundra receives less sunlight.

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2. The diagram below represents a food web composed of producers, consumers, and
decomposers.
A
B
D
Which group would represent the decomposer organisms?
1) A
2) B
3) C
4) D

Answers

Answer:

B

Explanation:

because that is the 2nd decompers

B group would represent the decomposer organisms. Therefore, the correct option is B.

What are Decomposers?

Decomposers are organisms that convert decaying organic matter into more basic components. They are an important component of the nutrition cycle because they convert complex chemical substances, such as nitrogen and carbon, into direct nutrients that can be absorbed by plants and other living things.

Some species of bacteria, fungi and insects are specialized decomposers. These organisms are essential to maintaining healthy soil because they aid in the recycling of nutrients and the breakdown of organic matter, which would otherwise build up and cause nutrient imbalances. Decomposers, which break down dead organisms and aid in the containment of pathogens, may be important in disease prevention.

Therefore, the correct option is B.

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Hard corals have a symbiotic relationship with species of microalgae that give the corals their color and provide up to 95% of the corals' energy needs by photosynthesis. Corals get the rest of their food by consuming plankton. The microalgae are damaged by changes in water temperature 2-3C lasting five weeks or more, causing a phenomenon called coral bleaching. What will most likely be the result of increased global temperature on the diversity of hard coral species?
Question 1 options:


The hard corals will make all of their own food.


The rate of photosynthesis in hard corals will increase.


The number of different hard coral species will decrease.


The hard corals will migrate to warmer waters.

Answers

Answer:

The hard corals will make all of their own food

Explanation:

your welcome...

The most likely result with increased global temperature would be ; ( A )

The hard corals will make their own food

Given that the relationship between the corals and the microalgae is symbiotic and not parasitic therefore when the microalgae are damaged the hard corals will most likely look for an alternative food source in order to continue surviving since they do not rely 100% on the plankton for their food. which is The hard corals will be able to make their own food by capturing preys like critters.

Hence we can conclude that the most likely result with increased global temperature would be  The hard corals will make their own food

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Please respond with true or false on all statements

Natural resource use is generally lower in developing
countries because people cannot afford many products.

The nations that consume the most oil are the nations that produce the most oil.

Pollution from discarded material degrades the land, air, and
water.

Consumerism leads to greater resource use, but it also leads
to more waste.

All of our electronic wastes are recycled within our country.

Answers

True
False
True
True
False

Which of the following are decomposers of an aquatic ecosystem?

A: sunlight and salt content

B: bacteria and fungi

C: aquatic plants and algae

D: sharks and eels

Answers

Aquatic plants and algae are the decomposers of an aquatic ecosystem.

Hope it helps you...

Please mark brainliest if it helps you...

Answered by Benjemin

Bacteria and Fungi are decomposers of an aquatic ecosystem. So, the correct option is (B).

What are Decomposers?

Decomposers are defined as organisms that break down dead or decaying organisms that undergo decomposition, a process possible only by certain kingdoms such as fungi.

Many decomposers are microscopic organisms, including protozoa and bacteria, while other decomposers are large enough to be seen without a microscope, including invertebrates as well as fungi, sometimes called detritivores, which include earthworms, Includes termites and millipedes.

In an aquatic ecosystem, producers are small plants like pistia which are consumed by primary consumers like aquatic insects, snails etc. where as primary consumers are consumed by secondary consumers like small fishes, frogs. The heron is a tertiary consumer that eats small fish and frogs. Bacteria and fungi are the decomposers in this ecosystem.

Thus, Bacteria and Fungi are decomposers of an aquatic ecosystem. So, the correct option is (B).

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a car is travelling with a constant velocity of 65.0m/s. how far will it travel in 0.30 minutes?
a. 3.61 meters
b. 19.50 meters
C. 1170.0 meters
d. 216.67 meters​

Answers

Answer:

B:19.50 meters

Explanation:

hope it helps!!

Answer:

answer is b 19.50 meters

In 1980, Mt. St. Helens erupted. The resulting lahar flowed over a wide area of the Cascade Mountains. Which of these statements DOES NOT describe the effect of the volcanic eruption?



A. It affected the living and nonliving components of the ecosystem.
B. It removed populations of mammals from the mountainside.
C. It led to succession on the mountainside.
D. It affected only nonliving components of the ecosystem.

Answers

The answer you looking for would be D

Correct answer is C

Have a great day!

Which agent is most likely the cause of chemical weathering to the tombstones in the cemetery? a)acid precipitation b)oxidation c)gravity​

Answers

I would believe it is A) Acid Precipitation

How does climate change affected plants and animal species on earth?

Answers

Answer:

Humans and wild animals face new challenges for survival because of climate change. More frequent and intense drought, storms, heat waves, rising sea levels, melting glaciers and warming oceans can directly harm animals, destroy the places they live, and wreak havoc on people's livelihoods and communities.

Explanation:

hope this helps you

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