Answer:
The correct answer is A. real-time PCR can measure the amount of DNA amplified as the reaction proceeds, while standard PCR cannot.
Explanation:
PCR (Polymerase Chain Reaction) is a molecular biology technique. Conventional PCR and real-time PCR are diferent types of PCR, and are both used to exponentially amplify DNA molecules. In both types, a polimerase enzyme is employed to sinthesize DNA copies. The main difference is that conventional PCR is qualitative whereas real-time PCR is quantitative. So, real-time PCR permits not only to detect and amplify DNA but also permits to perform a quantification of the target DNA. This is accomplished by measuring a fluorescence signal - which is directly the amount of DNA amplified- during the course of the reaction.
3.We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non- functional protein from the method used and results obtained this semester
Answer:
In the given case, the protein would have required to go through the process of post-translational modification, however, the mentioned protein would have been cultured in the prokaryotic system, the process of post-translational modification would have failed to take place. This step would be essential for the functionality of the protein. Thus, there is a need to culture it within the eukaryotic system.
The covalent and generally the enzymatic modification of proteins post its biosynthesis is termed as post-translational modification.
Food-borne illnesses can have substantial health and economic consequences. Correctly identify the steps that can lead to food-borne illness by Salmonella/Campylobacter and E. coli 0157:H7
1. Bloody diarrhea and hemolytic uremic syndrome can result
2. Live E. coli 0157:H7 bacteria are ingested
3. A Shiga-like toxin is produced
4. Incomplete cooking of raw hamburger fails to kill all pathogens
5. Live Salmonella or Campylobacter bacteria are ingested
6. Diarrhea, abdominal pain and nausea can result.
7. Incomplete cooking of poultry and fruits/vegetables fails to kill all pathogens
8. The pathogen multiplies in the intestinal tract for 2-48 days
Answer:
4,6,5,1,3
Explanation:
Type the word that appropriately completes each statement.
All fish reproduce sexually, but fertilization may occur in one of two ways.
In__________
fertilization, the female fish releases eggs in the water or on a surface. The male fish spreads
sperm over the eggs to fertilize them.
In__________
fertilization, the eggs are fertilized inside the female's body. The young develop there until the
female gives birth.
exExplanation: external internal
Hello, 3Coli here!
Here is your answer:
In external fertilization, the female fish releases eggs in the water or on a surface. The male fish spreads sperm over the eggs to fertilize them.
In internal fertilization, the eggs are fertilized inside the female's body. The young develop there until the female gives birth.
Hopefully, this helps! :D
Ask your question below!
why is energy flow through an ecosystem dependent upon continual energy transformations?
Answer:
Organisms must gather energy to survive.
Explanation:
Answer:
food
Explanation:
access the capabilities of animal and plant cell types owing the presence or absence of chloroplast and cell wall
Answer:At this point, it should be clear that eukaryotic cells have a more complex structure than do prokaryotic cells. Organelles allow for various functions to occur in the cell at the same time. Despite their fundamental similarities, there are some striking differences between animal and plant cells (see Figure 1).
Animal cells have centrosomes (or a pair of centrioles), and lysosomes, whereas plant cells do not. Plant cells have a cell wall, chloroplasts, plasmodesmata, and plastids used for storage, and a large central vacuole, whereas animal cells do not.
Explanation: pls give me brainest
Which of the following is NOT a property of water?
O A) It is a good solvent.
OB) It is denser when frozen than when liquid.
OC) It resists temperature changes.
OD) it is cohesive.
O El It can be found as a solid. liquid. or gas.
Answer:
It is denser when frozen than when liquid.
Explanation:
Ice is actually less dense than water. The lattice arrangement of ice allows water molecules to be more spread out than in a liquid, making ice less dense than water.
Hope that helps.
If any of y’all can give me the definition for these words I will give 5 stars ,like and brainlest or whatever it’s called
Answer:
rRNA - Ribosomal RNA, a molecular component of a ribosome, the cell's essential protein factory. Strictly speaking, ribosomal RNA (rRNA) does not make proteins. It makes polypeptides (assemblies of amino acids) that go to make up proteins
tRNA - Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps decode a messenger RNA (mRNA) sequence into a protein. tRNAs function at specific sites in the ribosome during translation, which is a process that synthesizes a protein from an mRNA molecule.
Explanation:
dy÷dx=(x-1)(x+3) at x=2
Answer:
[tex]\dfrac{dy}{dx}=\dfrac{4}{25}[/tex]
Explanation:
The given expression is :
[tex]y=\dfrac{(x-1)}{(x+3)}[/tex]
We need to find dy/dx at x = 2
[tex]\dfrac{dy}{dx}=\dfrac{d}{dx}(\dfrac{x-1}{x+3})\\\\=\dfrac{(x+3)\dfrac{d}{dx}(x-1)-(x-1)\dfrac{d}{dx}(x+3)}{(x+3)^2}\\\\=\dfrac{x+3-(x-1)}{(x+3)^2}\\\\=\dfrac{x+3-x+1}{(x+3)^2}\\\\\dfrac{dy}{dx}=\dfrac{4}{(x+3)^2}[/tex]
Put x = 2 in above expression
[tex]\dfrac{dy}{dx}|x=2=\dfrac{4}{(2+3)^2}\\\\=\dfrac{4}{25}[/tex]
Hence, the value at dy/dx is [tex]\dfrac{4}{25}[/tex]
Please help me I do not know how
which of the following best describes how the potential is affected by the structural change in the gene?
Answer:
A gene mutation is a permanent alteration in the DNA sequence that makes up a gene, such that the sequence differs from what is found in most people. Mutations range in size; they can affect anywhere from a single DNA building block (base pair) to a large segment of a chromosome that includes multiple genes.
Explanation: did the test and got it right
One codon contains the instructions to make
- 1 protein
- 1 amino acid
- RNA
Answer:
1 protein
Explanation:
Discuss the roles of the enzymes secreted by the pancreas during digestion.
The unit states that any animal that can be domesticated probably already has been. Explain the logic behind the statement and whether or not you find it convincing.
Answer:
Saying that if an animal can be domesticated that it probably already has been is saying that a lot of things are documented and if it has the capability to be domesticated or brought under human control then someone somewhere probably has. So I would personally find this convincing.
Explanation:
Hope that this helps and if so please mark me the brainliest. Peace and Love
One codon has the instructions to make
Answer:
1 protein
Explanation:
What effect does dietary protein have on the body?
Answer:
Even mostly or entirely plant-based diets can be high in protein. Protein is an essential part of a healthy diet. It helps to build and repair muscle, organs, and bones. High-protein diets have also been shown to be helpful with reducing fat, losing weight, increasing satiety, or a feeling of fullness, and retaining muscle.
In a food chain or web, is organism would be classified as a(n)
Answer:
producer
Explanation:
Pleaseeee help!!! (psychology edge) Androgens and estrogens are hormones that impact the sexual response cycle. Write about the four phases of that cycle and explain how the two types of hormones are involved.
Answer:
Females and males each have some amount of the sex hormones, androgens and estrogens. In males however, androgens like testosterone is more dominant and in females estrogens like estradiol are more dominant. The hormones both affect the sexual response cycle. The sexual response cycle has four phases: desire, arousal, orgasm and resolution. The desire phase can last anywhere from a few minutes to hours and includes physical changes like an increase in muscle tension, heartbeats quickening, flushed skin, and an increased flow of blood to genitals. The arousal phase extends to the brink of the orgasm phase, during this phase the changes experienced in the desire phase are intensified along with blood pressure increasing. The orgasm phase generally only lasts a few seconds and is the climax of the sexual response cycle, some characteristics include: Involuntary muscle contractions and spasms, blood pressure, breathing, and heart rate at their highest point, a release of built up sexual tension, and flushed skin. During the resolution phase the body returns to normal, often a sense of wellbeing and fatigue is achieved. To begin the cycle a male need a certain amount of the androgen, testosterone, but high levels in females tend to lead to more sexual thought and desires, typically though, women have relatively low levels of testosterone. In women, estrogens, such as estradiol are more common. Estrogens are produced when a girl starts puberty and helps her reproductive cycle begin. A high level of estrogen in men can lead to a reduced sex drive, loss of hair, or trouble focusing, but a normal level in men is crucial to proper erectile function.
Explanation:
I just submitted this, i hope it helps :)
Which microscope is used to check organelles of human cheek cell?
Answer:
4x or 10x
Explanation:
You need one of those to find the cells and then you can increase magnification
Which provide evidence for the general theory of relativity? Check all that apply
gravitational lensing
the constancy of the speed of light
the curvature of space-time
changes in Mercury's orbital path
O gravitational redshift
Answer:
Gravitational redshift
Explanation:
Gravitational redshift, changes in a planet's orbital path, gravitational lensing provides concrete evidence for the general theory of relativity. Hope this is helpful and the answer you were looking for!
Answer:
1 4 5
Explanation:
Choose THREE examples that correctly predict the effect of a change on the ecosystem.
А
A decrease in the kangaroo rat population will cause a decrease in the coyote
population
B
A decrease in the desert tortoise population will cause a decrease in the
rattlesnake population
Wc
A decrease in the rattlesnake population will cause an increase in the kangaroo
Answer:
The answer is option A "A decrease in the kangaroo rat population will cause a decrease in the coyote population"
Explanation:
Climate is a significant ecological impact on environments. Changing Climate influences biological systems in an variety of ways. For example, warming may constrain species to relocate to higher scopes or higher rises where temperatures are more helpful for their endurance.
Environmental change not just influences biological systems and species straightforwardly, it likewise communicates with other human stressors, for example, advancement. Although a few stressors cause just minor effects when acting alone, their combined effect may prompt emotional natural changes. For example, environmental change may worsen the pressure that land improvement puts on delicate water front zones. Also, as of late logged forested zones may get defenseless against disintegration if environmental change prompts increments in substantial down pour storms.
question 1. The construction of the Temple took seven years.
False True? question 2
Solomon sinned by allowing the corruption of God's people in the worship of idols.
True
False
Answer:
Question 1 true
question 2 true
Explanation:
Scenario 1
When you think of the American Southwest, you probably think of cacti, both big and small. If you were to
visit the American Southwest today, you might be disappointed with the lack of variation among cactus
species, especially right off the major highways. In 1926, Route 66 was the nation's first major highway to
allow visitors to visit the Southwest. Like many tourists, these visitors wanted to take home a memento of
their travels. Many people would pull over and begin looking for their own living souvenir. Since many of the
tourists did not have the necessary tools to extract a spiny cactus out of the soil, they usually had to make a
conscious decision about what type of cactus they should dig up. The cacti with too many spines were seen
as too difficult to dig out, and the visitors likely didn't have gloves to protect their hands. The cacti with too
few spines were seen as homely and were often overlooked. Therefore, the tourists found a cactus that was
between these two extremes. Over the last century, the phenotypic variation within these small cacti has
changed (at least in areas accessible by these highways).
What is a stimulus?
there are no options actually so plz help thank you
Answer:
In general, a stimulus is something that provokes or causes an action or response, as in Failing that test was the stimulus I needed to start studying harder. The plural of stimulus is stimuli. Its verb form is stimulate, which typically means to spur into action or to invigorate.
Explanation:
Answer:
In physiology, a stimulus is a detectable change in the physical or chemical structure of an organism's internal or external environment. The ability of an organism or organ to detect external stimuli, so that an appropriate reaction can be made, is called sensitivity
Explanation:
it just is
What is an essential characteristic of members of a given species?
the ability to produce eggs or live offspring without sexual reproduction
the ability to reproduce with members of another species
the ability to reproduce with one another and have fertile offspring
the ability to reproduce with organisms outside of their local gene pool
Answer:
the ability to reproduce with one another and have fertile offspring.
Explanation:
An essential characteristic of the members of a given species is the ability to reproduce with one another and have fertile offspring. Thus, the correct option is C.
What are the characteristics of a species?A species is a group of organisms which are living in an area with similar characteristics which are capable of exchanging genes and chromosomes and produce new organism similar to itself. The species are important as it maintains the equilibrium in the ecosystem.
A species can be defined as the largest group of organisms with the two individuals of the appropriate sexes can produce a fertile offspring, typically through sexual reproduction. The species can also be defined on the basis of their karyotype, DNA sequence, morphology, behavior or ecological niche.
Therefore, the correct option is C.
Learn more about Species here:
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For what reasons could a theory be changed or replaced? Check all that apply.
A new, untested hypothesis is proposed.
New information is discovered that does not support the theory.
A single experiment produces results that differ from all previous experiments.
New technology is developed that provides more information about the theory.
New experimental methods are designed to test a theory.
1. New technology is developed that provides more information about the theory.
2. New experimental methods are designed to test a theory.
3. A single experiment produces results that differ from all previous experiments.
im not sure about #3 but it could be it
Answer:
A. New technology is developed that provides more information about the theory.
B. New experimental methods are designed to test a theory.
D. New information is discovered that does not support the theory.
Explanation:
just did it
The correct sequence for the general adaptation syndrome is:_________
a) Exhaustion phase, alarm phase and resistance phase
b) Alarm phase, resistance phase and exhaustion phase
c) Resistance phase, exhaustion phase and alarm phase
d) Alarm phase, exhaustion phase and resistance phase
Answer:
The correct sequence for the general adaptation syndrome is: b) Alarm phase, resistance phase and exhaustion phase
Explanation:
The general adaptation syndrome describes what happens to our body when it is under stress.
The first phase is the Alarm phase. In this stage, the body activates the fight or flight response, causing an increase in adrenaline and cortisol, blood pressure, respiratory frequency, amongst other things that prepare us to react in a situation that we perceive as dangerous.
The second phase is the resistance one. During this phase, the body undergoes a different process that adapts it to manage the stress, with an elevated amount of cortisol and blood pressure.
The third phase is the exhaustion phase. If the stress levels continue to be high, the body passes to this stage. The body is exhausted and cannot keep dealing with stress, which causes mental and physical problems such as anxiety, depression, fatigue, stress-related illnesses, among other symptoms.
in chemical reactions, bonds blank in reactants.
List 2 natural hazards
due to Earth's internal
convection.
1. Chromosomes are made of
and
Answer:
protein and DNA
Explanation:
What are the properties of water that you can observe when transpiration takes place?
Answer:
When water evaporates through the leaves, a pull is created through the xylem, and water moves back to the leaves. This is known as the transpiration pull.