Select all that correctly apply to the human pedigree shown below.
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Individual #2 is heterozygous..
The pedigree represents dominant inheritance.
Individual #3 is heterozygous.
The pedigree represents recessive inheritance.
The pedigree represents x-linked recessive inheritance.
Answers
Pedigrees are used to get inheritance pattern of a gene and to determine possible genotypes among the family members. Correct options are 1) Individual #2 is heterozygous. 4) The pedigree represents recessive inheritance.
What is a pedigree?
A Pedigree is the representation of a family's history. This graph is used to track a trait through different generations, and analyze the inheritance pattern of a particular gene and its expression.
It is a tool used to understand how genes are transmitted from the parental generation to the descendants, and what are the probabilities of inheriting them.
Pedigree interpretation.
Family members→ Individuals are represented with geometrical figures.
→ Males are squares
→ Females are circles
Trait/Phenotype→ Healthy/normal/not affected individuals are represented with empty figures
→ Affected/mutated individuals are represented with solid black figures
Generations→ Each file is represented with a roman number, indicating the Generation.
In the e xposed pedigree,
Generation I
The man is affected (black square) - Individual 1The woman is not affected (empty circle). - Individual 2The woman must be homozygous dominant, since they had all healthy children, but affected grandchildren. This means the generation II are carriers, and the affection was expressed in the third generation.
Generation II
All healthy individualsThe two daughters and the son from the previous couple are heterozygousThe man that married daughter 2 is also heterozygous. You can tell this because they had affected children (black figures). The two remaining individuals might be either homozygous dominant or heterozygous.Generation III
Children from couples one a three (generation II) are all healthy individuals. They might be either heterozygous or homozygouys dominant.
Two children from the second couple (generation II) are healthy and two are affected. Solid black circles are affected individuals, while empty figures are healthy individuals.
Individual #2 is heterozygous. CORRECTThe pedigree represents dominant inheritance. INCORRECTIndividual #3 is heterozygous. INCORRECTThe pedigree represents recessive inheritance. CORRECTThe pedigree represents x-linked recessive inheritance. INCORRECTYou can learn more about pedigrees at
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10mg/1ml pure herring sperm diluted with 10ml sterile distilled water to C1 of 1000µg/ml. C1 Concentration of stock (1000µg/ml) V1 Volume of stock needed (µl) C2 Final Concentration (µg/ml) O 100 80 75 70 60 50 30 25 5 V2 Final volume (2,000μl) 2,000 2,000 2,000 2,000 2,000 2,000 2,000 2,000 2,000 1,000 1,000 1,000 1,000 1,000 1,000 1,000 1,000 1,000 Volume of H₂0 required (μl) Use water as a diluent and blank Mix each standard by inversion and measure the absorbance at 260nm (A260) with a UV - compatible cuvette. Graph your standard curve. On the graph include coefficient of regression (R²) and line graph equation (Y = mx + C).
Answers
Answer:
To create a standard curve using the provided data, you will need to dilute the pure herring sperm stock solution to various concentrations. The table below shows the calculations for each dilution:C1 Concentration of Stock (µg/ml)V1 Volume of Stock Needed (µl)C2 Final Concentration (µg/ml)V2 Final Volume (µl)Volume of H₂O Required (µl)100010010002000190010008080020001920100075750200019251000707002000193010006060020001940100050500200019501000303002000197010002525020001975100055020001995To prepare each standard, you will take the corresponding volume of the stock solution and dilute it with the calculated volume of sterile distilled water. For example, to prepare the first standard with a final concentration of 1000 µg/ml, take 100 µl of the pure herring sperm stock solution and add 1900 µl of sterile distilled water.Once you have prepared each standard, you can measure the absorbance at 260nm (A260) using a UV-compatible cuvette. Record the absorbance values for each standard.To create the standard curve, plot the concentrations (C2) on the x-axis and the absorbance values (A260) on the y-axis. Fit a linear regression line to the data points. The equation of the line will be in the form Y = mx + C, where Y is the absorbance, m is the slope of the line, x is the concentration, and C is the y-intercept.Calculate the coefficient of regression (R²) to assess the linearity of the curve. The R² value ranges from 0 to 1, with 1 indicating a perfect fit.Please note that I cannot make graph for you directly in this text-based format. You will need to input the data into a graphing software or spreadsheet program to create the graph, calculate the regression line equation, and determine the coefficient of regression (R²).