Question 2
Give three sources of nitrogen during purine biosynthesis by de
novo pathway
State the five stages of protein synthesis in their respective
chronological order
List 4 types of post-transla

Answers

Answer 1

Question 2: i. Three sources of nitrogen during purine biosynthesis by the de novo pathway are glutamine, glycine, and aspartate.

The de novo pathway is the process by which purine molecules are synthesized from simple precursors. In this pathway, nitrogen atoms are incorporated into the purine ring structure. Glutamine, an amino acid, provides an amino group (NH2) that contributes nitrogen atoms to the purine ring. Glycine provides a carbon and nitrogen atom, which are also incorporated into the ring. Aspartate contributes a carbon and nitrogen atom as well. These nitrogen-containing molecules serve as building blocks for the synthesis of purines, which are essential components of nucleotides.

ii. The five stages of protein synthesis in their respective chronological order are transcription, RNA processing, translation initiation, translation elongation, and translation termination.

Protein synthesis involves the conversion of the genetic information encoded in DNA into functional proteins. The process begins with transcription, where a DNA segment is transcribed into a complementary RNA molecule. Following transcription, RNA processing modifies the RNA molecule by removing introns and adding a cap and tail.

The processed mRNA then undergoes translation initiation, which involves the assembly of ribosomes and the recruitment of the first aminoacyl-tRNA. During translation elongation, amino acids are added to the growing polypeptide chain based on the codons in the mRNA. Finally, translation termination occurs when a stop codon is reached, leading to the release of the completed polypeptide chain.

iii. Four types of post-translational modifications that a polypeptide undergoes before maturing into a functional protein are phosphorylation, glycosylation, acetylation, and proteolytic cleavage.

Post-translational modifications (PTMs) are chemical modifications that occur on a polypeptide chain after translation. These modifications can alter the structure, function, and localization of proteins. Phosphorylation is the addition of a phosphate group to specific amino acids, typically serine, threonine, or tyrosine, and is crucial for signaling and regulation of protein activity.

Glycosylation involves the addition of sugar molecules to certain amino acids, impacting protein folding, stability, and cell recognition. Acetylation is the addition of an acetyl group to lysine residues and can influence protein-protein interactions and gene expression.

Proteolytic cleavage involves the removal of specific peptide segments from the polypeptide chain by proteolytic enzymes, resulting in the production of mature and functional proteins. These PTMs greatly expand the functional diversity of proteins and contribute to their regulation and activity in various cellular processes.

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Complete question:

Question 2

i. Give three sources of nitrogen during purine biosynthesis by de novo pathway

ii. State the five stages of protein synthesis in their respective chronological order

iii. List 4 types of post-translational modifications that a polypeptide undergoes before maturing into a functional protein


Related Questions

describe the relationship in chemical and physical the sturcture of L-Dopa and the decarboxylase? how do they interact with eachother?

Answers

L-Dopa, a chemical compound, interacts with the enzyme decarboxylase, which removes a carboxyl group from L-Dopa, converting it into dopamine. This interaction is significant for increasing dopamine levels in the brain and is essential in the treatment of Parkinson's disease.

L-Dopa, also known as Levodopa, is a chemical compound that serves as a precursor for the neurotransmitter dopamine. It is used as a medication for treating Parkinson's disease. L-Dopa has a specific chemical structure that allows it to cross the blood-brain barrier, where it is converted into dopamine by the enzyme decarboxylase.

Decarboxylase is an enzyme that catalyzes the removal of a carboxyl group from a molecule. In the case of L-Dopa, decarboxylase removes the carboxyl group, converting it into dopamine. This interaction between L-Dopa and decarboxylase is crucial for increasing dopamine levels in the brain, as dopamine deficiency is a characteristic feature of Parkinson's disease.

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What type of cells possess unlimited proliferation potential, have the capacity to self- renew, and can give rise to all cells within an organism? Question 2. Which laboratory method can be used to quantify levels of mRNAs expressed in samples of two different types of stem cells? Question 3. A cell that can differentiate into any cell within the same lineage is known as: Question 4. How did the researchers Kazutoshi Takahasi and Shinya Yamanaka accomplish cellular reprogramming of mouse fibroblasts in their 2006 publication in Cell?

Answers

The cells that possess unlimited proliferation potential, have the capacity to self-renew, and can give rise to all cells within an organism are known as stem cells.

1. The laboratory method that can be used to quantify levels of mRNAs expressed in samples of two different types of stem cells is known as Reverse transcription polymerase chain reaction (RT-PCR).

2. The cell that can differentiate into any cell within the same lineage is known as a multipotent stem cell. Multipotent stem cells have the capacity to differentiate into various cell types within the same lineage or tissue, but not all cell types.

3. The researchers Kazutoshi Takahashi and Shinya Yamanaka accomplished cellular reprogramming of mouse fibroblasts in their 2006 publication in Cell by inducing the expression of four transcription factors: Oct4, Sox2, Klf4, and c-Myc.

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Which kinds of nonhuman primates seem to use visual cues other than that of an actual animal, but made by other animals to learn about the location of that animal? a) vervet monkeys b) neither vervet monkeys nor chimpanzees c) both vervet monkeys and chimpanzees d) chimpanzees

Answers

Studies have shown that both vervet monkeys and chimpanzees are able to use visual cues other than that of an actual animal but made by other animals to learn about the location of that animal.

The use of such visual cues has implications for learning and social interactions among nonhuman primates.

Primate communication is an important part of the social behavior of these animals.

Nonhuman primates use a range of communication methods such as visual cues, auditory signals, touch, and smell to convey information to members of their own and other species.

Among these communication methods, visual cues are particularly important for nonhuman primates.

They can learn about the location of predators or potential prey by watching the behavior of other animals around them.

Several species of primates, including vervet monkeys and chimpanzees, have been found to use visual cues such as predator models or predator dummies to learn about the presence of predators in their environment.

In one study, researchers found that both vervet monkeys and chimpanzees could learn about the location of predators by observing the behavior of other animals around them.

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Match the four common fungal diseases and their causative agents. Histoplasma capsulatum [Choose ] Tinea species [Choose] Candida [ Choose] Aspergillus [Choose ]

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Match the four common fungal diseases and their causative agents. Histoplasma capsulatum - Histoplasmosis, Tinea species - Dermatophytosis (ringworm), Candida - Candidiasis, Aspergillus - Aspergillosis.

Diseases are abnormal conditions or disorders that affect the normal functioning of the body, leading to physical or mental impairments. There are numerous types of diseases, including infectious diseases caused by pathogens like bacteria, viruses, or parasites (e.g., influenza, malaria); chronic diseases characterized by long-term persistence or recurring symptoms (e.g., diabetes, hypertension); genetic disorders caused by inherited genetic mutations (e.g., cystic fibrosis, sickle cell anemia); autoimmune diseases where the immune system attacks the body's own tissues (e.g., rheumatoid arthritis, lupus); and many others affecting various organs and systems in the body. Accurate diagnosis, treatment, and preventive measures are vital in managing diseases and promoting overall health.

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The Class of antibody produced during B cell maturation is determined at the B (type of nucleic acid) level while the form of antibody, either membrane bound or secreted, is determined at the to express IgM or or IgD is made at the level of the process called D level. The decision through a . Class switching occurs at the level of the E

Answers


The class of antibody produced during B cell maturation is determined at the B (DNA) level, while the form of antibody, either membrane-bound or secreted, is determined at the level of the process called the D level. The decision to express IgM or IgD is made at the D level. Class switching occurs at the level of the E.



The type of nucleic acid present in B-cells is DNA. The class of antibody that is generated during B-cell maturation is determined at the DNA level. In the heavy chain constant region genes, the coding segment for the Fc region determines the class of the antibody produced.

The form of the antibody (whether it is membrane-bound or secreted) is determined at the level of the process called the D level. The decision to express either IgM or IgD is made at this level.

Class switching occurs at the level of the E (epsilon) heavy-chain gene, leading to the production of antibodies with different effector functions. This is a process that occurs after the generation of the initial antibody during B-cell maturation.


B cells are one of the major types of lymphocytes involved in the adaptive immune system. B-cell maturation occurs in the bone marrow and results in the generation of B cells that are capable of producing antibodies that are specific to a particular antigen.

During B-cell maturation, a series of genetic rearrangements occur that result in the expression of a unique immunoglobulin (Ig) molecule on the surface of the cell.

The immunoglobulin molecule is composed of two heavy chains and two light chains, which are held together by disulfide bonds. Each heavy and light chain has a variable region, which is responsible for binding to antigen, and a constant region, which determines the class of the antibody produced.

The class of antibody produced during B-cell maturation is determined at the B (DNA) level, while the form of antibody, either membrane-bound or secreted, is determined at the level of the process called the D level. The decision to express either IgM or IgD is made at this level.

Class switching occurs at the level of the E (epsilon) heavy-chain gene, leading to the production of antibodies with different effector functions. This is a process that occurs after the generation of the initial antibody during B-cell maturation.

It involves the deletion of the DNA between the initial constant region gene and the new constant region gene, followed by recombination with the new constant region gene.

This results in the production of an antibody with a different heavy-chain constant region, which can result in different effector functions such as opsonization or complement fixation.

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Consider a strain of E. coli in which, after the glucose in the medium is exhausted, the order of preference for the following sugars, from most preferred to least preferred, was maltose, lactose, melibiose, trehalose, and raffinose. Which operon would require the highest concentration of CRP-cAMP in order to be fully induced?

Answers

The operon for raffinose metabolism would require the highest concentration of CRP-cAMP in order to be fully induced in this E. coli strain.

To determine which operon would require the highest concentration of CRP-cAMP (cyclic AMP) to be fully induced in the given strain of E. coli, we need to understand the regulatory role of CRP-cAMP and the sugar preference of the strain.

CRP (cAMP receptor protein) is a regulatory protein in E. coli that binds to cAMP and interacts with specific DNA sequences called cAMP response elements (CREs) or CRP-binding sites. When CRP-cAMP binds to these sites, it can activate or enhance the transcription of target genes.

In the presence of glucose, E. coli typically exhibits catabolite repression, where the utilization of alternative sugars is repressed until glucose is depleted. However, once glucose is exhausted, CRP-cAMP levels increase, enabling the induction of operons responsible for metabolizing other sugars.

Based on the order of sugar preference given (maltose, lactose, melibiose, trehalose, and raffinose), the operon that requires the highest concentration of CRP-cAMP to be fully induced would be the operon responsible for metabolizing raffinose.

Therefore, the operon for raffinose metabolism would require the highest concentration of CRP-cAMP in order to be fully induced in this E. coli strain.

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A virus that has entered the lysogenic cycle: Cannot replicate its genome Can only replicate its genome when environmental conditions are favorable Replicates its genome when its host cell replicates Can only replicate its genome when it exits the lysogenic cycle and enters the lytic cycle

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A virus that has entered the lysogenic cycle: Cannot replicate its genome Can only replicate its genome when environmental conditions are favorable Replicates its genome when its host cell replicates Can only replicate its genome when it exits A virus that has entered the lysogenic cycle replicates its genome when its host cell replicates.

In the lysogenic cycle, a virus integrates its genetic material into the host cell's genome and remains dormant. During this phase, the virus does not immediately replicate its genome but instead relies on the host cell's replication machinery to replicate its genetic material along with the host's DNA. When the host cell undergoes replication, the viral genome is also replicated, allowing it to be passed on to daughter cells. Therefore, a virus in the lysogenic cycle replicates its genome when its host cell replicates.

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Use the ions and match them to the appropriate scenario. What ion is important in muscle contraction cycle? [Choose his ion passes through the resting neuron's cell membrane the easiest. [Choose [Choo

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The ion important in the muscle contraction cycle is calcium (Ca^{2+}). The ion that passes through the resting neuron's cell membrane the easiest is potassium ([tex]K^{+}[/tex]).

Muscle Contraction Cycle: Calcium ([tex]Ca^{2+}[/tex]) is a crucial ion in the muscle contraction cycle. During muscle contraction, calcium ions are released from the sarcoplasmic reticulum in response to a neural signal. The binding of calcium to the protein troponin triggers a series of events that allow actin and myosin to interact, leading to muscle contraction.

Resting Neuron's Cell Membrane: The ion that passes through the resting neuron's cell membrane the easiest is potassium (K^{+}). Neurons have specialized channels, called potassium channels, that allow potassium ions to move in and out of the cell. These channels are responsible for maintaining the resting membrane potential of the neuron. At rest, the neuron's membrane is more permeable to potassium ions, and they tend to move out of the cell, leading to a negative charge inside the neuron.

The movement of potassium ions contributes to the generation and propagation of action potentials in neurons. When an action potential is initiated, there is a temporary increase in the permeability of the cell membrane to sodium ions ([tex]Na^{+}[/tex]), allowing them to enter the cell and depolarize the membrane. However, during the resting state, potassium ions play a key role in maintaining the resting membrane potential.

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For each embryonic tissue type, write one organ or differentiated cell type that is derived from that tissue. (8)
Neural Ectoderm ________________________
Epidermis ________________________
Neural Crest ________________________
Somite _____ ___________________

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producir elmelanina, que determina el color de la piel y protege contra los rayos UV. En resumen, la epidermis del ectodermo protege el cuerpo y el sistema nervioso central procesa y transmite información en el cuerpo.

Neural Ectoderm: El cerebro y la columna vertebral son las estructuras del sistema nervioso central (CNS) responsables de procesar y transmitir información en el cuerpo. Los neuronas, que son los componentes esenciales del sistema nervioso, y las células gliales, que brindan apoyo e insulación a los neuronas, son algunos de los diversos tipos de células especializadas que componen estos órganos.La capa exterior de la piel es la epidermis, que proviene del ectodermo. It functions as a barrier that protects against external factors like pathogens, UV radiation, and dehydration. El dermis está formado por varios tipos de células, incluidos los keratinocitos que producen el keratino proteico, que da a la piel su fuerza y propiedades impermeables. Los melanócitos son otras células presentes en la epidermis y son responsables de

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The neural ectoderm gives rise to the central and peripheral nervous system, the epidermis gives rise to the skin and associated structures, the neural crest gives rise to several cell types, and the somite gives rise to muscle and bone.

For each embryonic tissue type, write one organ or differentiated cell type that is derived from that tissue. (8)The eight embryonic tissues and the organs or differentiated cell types derived from them are as follows:1. Neural Ectoderm: The neural ectoderm is a group of cells that differentiate into the central and peripheral nervous systems.2. Epidermis: The epidermis is the outermost layer of skin that protects the body from the environment and helps regulate body temperature.3. Neural Crest: The neural crest gives rise to several cell types including sensory and autonomic ganglia, Schwann cells, and adrenal medulla cells.4. Somite: The somite is a group of cells that differentiate into muscle and bone.

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Define and be able to identify the following terms as they relate to the hair: a. Shaft b. Root C. Matrix d. Hair follicle e. Arrector pili muscle Define and be able to identify the following terms as

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The arrector pili muscle is responsible for causing the hair to stand upright when it contracts.As it relates to hair, the following terms can be defined and identified:

a. ShaftThe shaft of the hair is the portion of the hair that is visible on the surface of the skin. The shaft is the part of the hair that we can see, and it is made up of dead skin cells that have become keratinized, or hardened.

b. RootThe root of the hair is the part of the hair that is located beneath the skin's surface. The root is the part of the hair that is responsible for producing the hair shaft.

c. MatrixThe matrix is a layer of cells located at the base of the hair follicle. The matrix is responsible for producing new hair cells, which will eventually become part of the hair shaft.

d. Hair follicleThe hair follicle is a structure located beneath the skin's surface that produces hair. The hair follicle is responsible for producing and maintaining the hair shaft.e. Arrector pili muscleThe arrector pili muscle is a small muscle located at the base of each hair follicle.

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Mendel crossed true-breeding purple-flowered plants with true-breeding white-flowered plants, and all of the resulting offspring produced purple flowers. The allele for purple flowers is _____.
a) segregated
b) monohybrid
c) dominant
d) recessive

Answers

The answer to your question is option C. Dominant. Mendel conducted numerous experiments using the garden pea (Pisum sativum) to discover the basic principles of inheritance. He found that a single gene pair controls a single trait, one member of the pair being inherited from the male parent and the other from the female parent

Mendel conducted numerous experiments using the garden pea (Pisum sativum) to discover the basic principles of inheritance. He found that a single gene pair controls a single trait, one member of the pair being inherited from the male parent and the other from the female parent. In Mendel's experiment, he crossed true-breeding purple-flowered plants with true-breeding white-flowered plants, resulting in all of the offspring producing purple flowers. Mendel also discovered that the traits were inherited in two separate units, one from each parent. These units are known as alleles.

An allele is one of two or more versions of a gene. Individuals receive two alleles for each gene, one from each parent. If the two alleles are the same, the individual is homozygous, whereas if the two alleles are different, the individual is heterozygous. When it comes to flower color, the allele for purple flowers is dominant over the allele for white flowers, which is recessive. As a result, all offspring produced purple flowers in Mendel's experiment. The answer to your question is option C. Dominant.

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describe lysogenic conversion and its significance
[10]

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Lysogenic conversion is a phenomenon in which a bacteriophage integrates its genetic material into the genome of its bacterial host, resulting in the transfer of new genes and traits to the bacterium.

Lysogenic conversion occurs when a temperate bacteriophage infects a bacterial cell and integrates its genetic material, called a prophage, into the bacterial genome. Unlike the lytic cycle, where the bacteriophage immediately lyses the host cell to release new viral particles, the prophage remains dormant within the bacterial chromosome. During this latent phase, the prophage is replicated along with the bacterial DNA during cell division.

Lysogenic conversion is significant because it allows for the transfer of new genetic material to the bacterial host. The integrated prophage can carry genes that encode for specific virulence factors or other advantageous traits. These genes can alter the behavior, metabolism, or pathogenicity of the bacterial host, enabling it to adapt to new environments, evade the host immune system, or enhance its ability to cause disease. Lysogenic conversion has been observed in various pathogenic bacteria, such as Vibrio cholerae, which acquires genes encoding cholera toxin through lysogeny, contributing to the severity of cholera infections.

Overall, lysogenic conversion plays a crucial role in bacterial evolution and the acquisition of virulence factors, providing a mechanism for bacteria to acquire new traits that can enhance their survival and pathogenic potential.

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Suppose you have a plentiful supply of oak leaves are about 49% carbon by weight. Recall our autotutorial "Soil Ecology and Organic Matter," where we calculated N surpluses (potential N mineralization) and N deficits (potential N immobilization) based on the C:N ratios of materials that one might incorporate into soils. We assumed that just 35% of C is assimilated into new tissue because 65% of C is lost as respiratory CO2, and that soil microorganisms assimilate C and N in a ratio of 10:1. Using these assumptions, please estimate the potential N mineralization or immobilization when 97 pounds of these oak leaves with C:N = 62:1 are incorporated into soil. If this number (in pounds of N) is a positive number (mineralization), then just write the number with no positive-sign. However, if this number (in pounds of N) is negative (immobilization), then please be sure to include the negative-sign! Your Answer:

Answers

Oak leaves are approximately 49 percent carbon by weight. We will estimate the potential N mineralization or immobilization when 97 pounds of these oak leaves with C:

where we calculated N surpluses (potential N mineralization) and N deficits (potential N immobilization) based on the C.

N = 62:1

are incorporated into the soil using the assumptions from the auto tutorial.

"Soil Ecology and Organic Matter,".

N ratios of materials that one might incorporate into soils.

We know that,

C:

N ratio for oak leaves is 62:

As per the given, just 35% of C is assimilated into new tissue because 65% of C is lost as respiratory CO2.

and soil microorganisms assimilate C and N in a ratio of 10:1.

Assuming a starting value of 97 l bs of oak leaves,

the carbon contained in them can be calculated as follows:97.

the potential N mineralization or immobilization can be calculated as follows:

47.53 l.

bs carbon * 0.35 = 16.64 l.

bs carbon in new tissue.

47.53 l.

bs carbon * 0.65 = 30.89 l.

bs respiratory CO2For 16.64 l.

bs of new tissue,

we can assume that the microorganisms will assimilate 1.664 l bs of N.

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The official sequencing of the human genome began in 1990 and took 13 years to finish. The composition of the genome was a big surprise regarding the percentage of the human genome containing coding genes. What was the surprise and provide three different types of non-coding DNA that were found in the human genome?

Answers

The surprise was that coding genes constitute only a small fraction of the human genome. It was found that only around 2% of the human genome encodes proteins.

The rest of the genome is composed of non-coding DNA. Some examples of non-coding DNA found in the human genome are as follows:1. Introns: These are the segments of DNA that lie between coding regions in a gene and are transcribed into RNA but are ultimately spliced out during RNA processing.2. Regulatory DNA: These sequences control when and how genes are expressed.

They include promoter regions, enhancers, and silencers.3. Transposable Elements: These are DNA sequences that can move around the genome.

They were once thought to be "junk DNA" but are now known to have important functions in gene regulation and evolution.

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Question 35 The enzyme responsible for digesting sucrose is known as sucrase which breaks sucrose down into O glucose and galactose O glucose and glucose O glucose and fructose O fructose and fructose

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The enzyme responsible for digesting sucrose is known as sucrase, which breaks sucrose down into glucose and fructose.

Sucrase is a type of enzyme called a carbohydrase that plays a crucial role in the digestion of sucrose, a disaccharide commonly found in many foods. When we consume sucrose, sucrase is produced in the small intestine to facilitate its breakdown. The enzyme sucrase acts on the glycosidic bond present in sucrose, which connects glucose and fructose molecules. By cleaving this bond, sucrase effectively splits sucrose into its constituent monosaccharides: glucose and fructose.

Once sucrose is broken down into glucose and fructose, these individual sugars can be readily absorbed by the small intestine and enter the bloodstream. From there, they are transported to various cells throughout the body to provide energy for cellular processes. The breakdown of sucrose by sucrase is an essential step in the digestion and absorption of carbohydrates, allowing our bodies to utilize the energy stored in this common dietary sugar.

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What would happen during DNA extraction process, if
you forgot to add in the soap solution?

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If the soap solution is forgotten during the DNA extraction process, it would likely result in inadequate lysis of the cell membrane and the release of DNA.

The soap solution, also known as a lysis buffer, is used to break down the lipid bilayer of the cell membrane, allowing the DNA to be released from the cells.

Without the soap solution, the cell membrane would remain intact, preventing efficient release of DNA. This would hinder the subsequent steps of the DNA extraction process, such as the denaturation and precipitation of proteins, as well as the separation of DNA from other cellular components. As a result, the yield of DNA would be significantly reduced, and the extraction process may not be successful.

It is important to follow the specific protocol and include all necessary reagents, including the soap solution or lysis buffer, to ensure successful DNA extraction and obtain high-quality DNA for further analysis.

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68 Anatomy and Physiology I MJB01 02 (Summer 2022) Which of the following organelles is responsible for the breakdown of organic compounds? Select one: a. Ribosomes b. Lysosomes c. Rough endoplasmic r

Answers

Lysosomes are organelles responsible for the breakdown of organic compounds. They are small spherical-shaped organelles, which are formed by the golgi complex, and contain digestive enzymes to break down organic macromolecules such as lipids, proteins, carbohydrates.

And nucleic acids into smaller molecules which can be utilized by the cell.Lysosomes are responsible for cellular autophagy, a process where damaged organelles are broken down and recycled. The membrane surrounding lysosomes protects the cell from the digestive enzymes contained within it.

From the golgi complex, lysosomes are formed and released into the cytoplasm. Lysosomes are essential for the cell to perform its functions efficiently and maintain its integrity. A disruption in lysosomal function can lead to various diseases such as lysosomal storage disorders, neurodegenerative disorders, and even cancer.

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Eventually, you are able to grow the chemolithoautotroph as well. Given what you know about the organism’s metabolism and the environment it came from, what should you change about the standard culturing conditions to promote the growth of this organism?
A) Lower the pH
B) Add more anaerobic electron acceptors
C) Expose the cells to sunlight
D) Add glucose
E) Grow the cells anaerobically

Answers

The metabolic pathway of chemolithoautotrophs is unique in the fact that these bacteria are able to survive without light, organic compounds, or oxygen as they gain their energy through the oxidation of inorganic compounds like nitrate, ammonia, and sulfur.

In order to promote the growth of chemolithoautotrophs, a few modifications can be made to the standard culturing conditions. The options are provided below:

1) Lower the pH: This condition won't be helpful in promoting the growth of the chemolithoautotrophs as most of the chemolithoautotrophs are found to grow at a neutral or an alkaline pH.

2) Add more anaerobic electron acceptors: This condition could be useful in promoting the growth of chemolithoautotrophs as most of these organisms require electron acceptors like CO2, NO2-, SO4-2, Fe2+, etc for their metabolism.

3) Expose the cells to sunlight: As chemolithoautotrophs are known to survive without light, this condition is not applicable.

4) Add glucose: This condition is not applicable as chemolithoautotrophs do not rely on organic compounds for their metabolism.

5) Grow the cells anaerobically: This condition could be useful in promoting the growth of chemolithoautotrophs as most of these organisms are found to grow in anaerobic conditions.

Therefore, growing the cells anaerobically could help in promoting the growth of the chemolithoautotroph.

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1. Which of the following molecule is mismatched?
A. mRNA: the order of nucleotides in this molecule determines
the identity of the amino acid dropped off
B. mRNA: site of translation when ribosomes a

Answers

The mismatched molecule is A. mRNA: the order of nucleotides in this molecule determines the identity of the amino acid dropped off.

The given statement is incorrect because it misrepresents the role of mRNA in protein synthesis. mRNA, or messenger RNA, is responsible for carrying the genetic information from the DNA to the ribosomes during protein synthesis.

The order of nucleotides in mRNA determines the sequence of amino acids that will be incorporated into a growing polypeptide chain during translation. Each group of three nucleotides, called a codon, codes for a specific amino acid.

The mRNA does not determine the identity of the amino acid dropped off; instead, it carries the instructions for assembling the amino acids in the correct order.The correct statement regarding mRNA is as follows: B. mRNA: site of translation when ribosomes generate proteins.

During translation, ribosomes attach to the mRNA molecule and move along its length, reading the codons and recruiting the appropriate amino acids to build a polypeptide chain.

The ribosomes act as the site of translation, facilitating the assembly of amino acids into a protein according to the instructions carried by the mRNA. Therefore, the correct match is B, where mRNA serves as the site of translation when ribosomes generate proteins.

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1. Please describe the journal of how starch becomes ATP molecules in a skeletal muscle cells. Describe the chemical, physical, and biological events occurs in the gastrointestinal, circulatory systems (3 points), and the molecular evens in the skeletal muscle cells (2 points). 2. Kidney function indicators: What is the source of albumin and hemoglobin in urine? (1 point) Explain based on the urine formation mechanisms why we have nearly no albumin and hemoglobin in healthy urine? (2 points) Why leukocyte is not considered as a kidney function indicator? (2 points) How does leukocyte get into the urine from bloodstream? (1 points)

Answers

1. Starch is broken down into glucose in the gastrointestinal system. Glucose is absorbed into the bloodstream and delivered to skeletal muscle cells. In the cells, glucose undergoes glycolysis to produce ATP through a series of chemical reactions.

ATP is then used for muscle contraction. This process involves both physical digestion in the gastrointestinal system and biological events in the circulatory system and skeletal muscle cells.

In the gastrointestinal system:

- Starch is hydrolyzed into glucose by enzymes like amylase.

- Glucose is absorbed into the bloodstream through the intestinal wall.

In the circulatory system:

- Glucose is transported in the bloodstream to the skeletal muscle cells.

In skeletal muscle cells:

- Glucose enters the cells through glucose transporters.

- Glycolysis occurs, breaking down glucose into pyruvate.

- Pyruvate is further converted into ATP through cellular respiration.

2. The source of albumin in urine is damaged kidney filtration membranes, and hemoglobin can appear in urine due to various medical conditions. Healthy urine has minimal albumin and hemoglobin because the kidneys efficiently filter and reabsorb these substances, preventing their excretion. Leukocytes are not considered kidney function indicators because their presence in urine is usually associated with urinary tract infections or other pathological conditions. Leukocytes can enter the urine from the bloodstream by crossing the damaged or inflamed kidney filtration membranes.

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Which of the following chromosome abnormalities (assume heterozygous for abnormality) lead to unusual metaphase alignment in mitosis? Why?
I. Paracentric inversions
II. Pericentric inversions
III. Large internal chromosomal deletions
IV. Reciprocal translocation

Answers

Among the chromosome abnormalities listed, the main condition that leads to unusual metaphase alignment in mitosis is the reciprocal translocation.

Reciprocal translocation involves the exchange of genetic material between non-homologous chromosomes. During mitosis, when chromosomes align along the metaphase plate, translocated chromosomes can exhibit abnormal alignment due to the altered position of the genes involved in the translocation.

In reciprocal translocation, two non-homologous chromosomes break and exchange segments, leading to a rearrangement of genetic material. As a result, the genes on the translocated chromosomes may not align properly during metaphase. This misalignment can disrupt the normal pairing of homologous chromosomes and interfere with the separation of chromosomes during anaphase, potentially resulting in errors in chromosome distribution and aneuploidy.

It's important to note that paracentric inversions, pericentric inversions, and large internal chromosomal deletions do not directly cause unusual metaphase alignment in mitosis. These abnormalities may lead to other effects such as disrupted gene function or changes in chromosome structure, but their impact on metaphase alignment is less pronounced compared to reciprocal translocations.

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Question 6 -2.5 points Trichloroacetic acid is a potent denaturant of proteins. The process of protein denaturation involves a. The disruption of many of the non-covalent bonds that hold the protein i

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The answer to the given question is protein structure and function. The disruption of many of the non-covalent bonds that hold the protein in its native conformation is involved in the process of protein denaturation.

Trichloroacetic acid is a powerful denaturant that is used to denature proteins. It has a high solubility in water and organic solvents, making it a useful reagent in the study of proteins. Proteins are complex biomolecules that perform a variety of functions in living organisms.

The 3D conformation of a protein is critical to its function. The process of protein denaturation involves the disruption of many of the non-covalent bonds that hold the protein in its native conformation. This results in a loss of the protein's function and structural integrity.

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An organism takes up 4 subdivisions (or 4 o.s/4 ocular spaces) when viewed with the 100x objective. How big is the organism?

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The organism's size can't be determined without additional data about the field of view and magnification of the microscope.

An organism takes up 4 subdivisions (or 4 o.s/4 ocular spaces) when viewed with the 100x objective. In determining the size of an organism, the field of view must first be determined. The field of view is the region of the slide that is visible through the microscope ocular and objective lenses.

Field of view diameter can be calculated using the formula:

FOV1 x Mag1

= FOV2 x Mag2

Where FOV1 is the diameter of the low-power field of view, Mag1 is the low-power magnification, FOV2 is the diameter of the high-power field of view, and Mag2 is the high-power magnification.

Since the organism can be seen in 4 subdivisions when viewed with the 100x objective, it must be calculated based on the microscope's magnification and field of view.

Therefore, the organism's size can't be determined without additional data about the field of view and magnification of the microscope.

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1. Discuss how carbon sources will affect the microbes that grow in the Winogradskycolumn.
2. If samples were extracted from the various layers of all the columns, where would you find photosynthetic organisms such as cyanobacteria and algae? Explain why

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Additionally, these organisms require oxygen for photosynthesis, which is also available in the upper layers of the column. Therefore, the presence of these photosynthetic organisms in the upper layer of the Winogradsky column indicates a well-oxygenated environment with sufficient light for photosynthesis to occur.

1. Carbon sources will affect the microbes that grow in the Winogradsky columnCarbon sources are key to the survival and growth of microbes in the Winogradsky column. In this column, the presence of various carbon sources will affect the types of microbes that grow in different areas. Some carbon sources include carbohydrates, fatty acids, amino acids, and organic acids such as citric acid, malic acid, and succinic acid. The availability of these different carbon sources will determine which microbes can grow, as different microbes have different metabolic pathways and are capable of using different carbon sources.2. Cyanobacteria and algae in the Winogradsky columnPhotosynthetic organisms such as cyanobacteria and algae will be found in the upper layer of the Winogradsky column. This is because they require sunlight to carry out photosynthesis, which is only available in the uppermost layers of the column. Additionally, these organisms require oxygen for photosynthesis, which is also available in the upper layers of the column. Therefore, the presence of these photosynthetic organisms in the upper layer of the Winogradsky column indicates a well-oxygenated environment with sufficient light for photosynthesis to occur.

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from Guppy Genes Part 1: A.) What hypothesis was John Endlec testing with this experiment? What did he expect to find if his hypothesis was supported? B.) Describe the selective force that is likely driving the changes. (Remember that there are no longer major predators on adult guppies in "Intro.") Tom Guppy Genes Part 2: C.) What hypothesis was Grether testing with this experiment? What did he expect to find if his hypothesis was supported? D.) Why did Grether use brothers in the three treatments instead of unrelated guppies?

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The above question is asked from Guppy Genes Part 1 in 4 sections, for A, his hypothesis was that female gupples have a [reference of males with bright orange spots, for B it is sexual selection.

For C to see the presence of predators influences guppy coloration and for D genetic variation.

A.) John Endlec's experiment aimed to test the hypothesis that female guppies have a preference for males with bright orange spots. If his hypothesis was supported, he expected to find that female guppies displayed a stronger attraction towards males with more vibrant orange spots compared to those with duller or no spots.

B.) The primary selective force driving changes in guppy coloration is sexual selection. In the absence of major predators on adult guppies, mate choice and competition for mates become prominent factors. Bright orange spots in male guppies may signal genetic quality, good health, or the ability to acquire resources. Female guppies that choose brighter-spotted mates may gain advantages for their offspring's survival and reproductive success.

C.) Grether's experiment aimed to test the hypothesis that the presence of predators influences guppy coloration. If his hypothesis was supported, he expected to find that guppies in predator-rich environments exhibited more subdued coloration compared to those in predator-free environments.

D.) Grether used brothers in the three treatments instead of unrelated guppies to control for genetic variation. By doing so, he ensured that any observed differences in coloration between the treatments could be attributed to the presence or absence of predators rather than genetic differences between unrelated individuals. This control allowed for a more precise examination of the specific impact of predator presence on guppy coloration.

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gigas (gig, fly TSC2) mutant clones the corresponding WT twin spots were generated during Drosophila eye development, determine whether the following statements are true or false:
A. gig mutant clones will be larger than twin spots with larger cells
B. gig mutant clones will be larger than twin spots with more cells
C. gig mutant clones will be smaller than twin spots with smaller cells

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The true or false of the following statements for gigas (gig, fly TSC2) mutant clones and their corresponding WT twin spots during Drosophila eye development are: A. gig mutant clones will be larger than twin spots with larger cells - False. B. gig mutant clones will be larger than twin spots with more cells - True. C. gig mutant clones will be smaller than twin spots with smaller cells - False.

The true or false of the following statements for gigas (gig, fly TSC2) mutant clones and their corresponding WT twin spots during Drosophila eye development are:

A. gig mutant clones will be larger than twin spots with larger cells - False.

B. gig mutant clones will be larger than twin spots with more cells - True

C. gig mutant clones will be smaller than twin spots with smaller cells - False.

In Drosophila melanogaster eye, it has been shown that Tuberous Sclerosis Complex (TSC) regulates cell size and number through the protein kinase complex Target of Rapamycin Complex 1 (TORC1) and the transcription factor Myc.

A reduction in TSC function results in larger cells with more nucleoli, a phenotype that is commonly used to identify cells with elevated TORC1 signaling. When determining if the statements A, B, and C are true or false, the following explanation can be used:

A. False. Gig mutant clones will not be larger than twin spots with larger cells because, in this scenario, cell size is not altered.

B. True. Gig mutant clones will be larger than twin spots with more cells because the function of the gig is associated with cell number, as described in the explanation.

C. False. Gig mutant clones will not be smaller than twin spots with smaller cells because the function of the gig is not related to cell size.

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Which of the following issues would not be included in a food safety management system?
The number of pieces of egg shell in powdered milk. The heating instructions on the package say "do not microwave this food" but the consumer microwaves and then eats the food. The concentration of N2(g) in a modified atmosphere package. The receiving temperature of a fluid milk product arriving at an ice cream manufacturer.

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This issue would be included in a food safety management system.The heating instructions on the package say "do not microwave this food" but the consumer microwaves and then eats the food is not a food safety issue.

A food safety management system (FSMS) is a systematic method for identifying and preventing hazards in food production and distribution. It is designed to ensure that food products are safe for human consumption.

The following issue, "The heating instructions on the package say "do not microwave this food" but the consumer microwaves and then eats the food" would not be included in a food safety management system.

Below are the reasons why the other options would be included in a food safety management system and the fourth option would not be included in an FSMS:

1. The number of pieces of eggshell in powdered milk: Eggshell pieces in powdered milk may cause physical contamination of the product.

As a result, this issue would be included in a food safety management system.

2. The concentration of N2(g) in a modified atmosphere package: The atmosphere in modified atmosphere packages is altered to extend the shelf life of food products. The concentration of N2(g) is closely monitored to ensure that it meets specific requirements.

As a result, this issue would be included in a food safety management system.

3. The receiving temperature of a fluid milk product arriving at an ice cream manufacturer: The temperature at which milk is stored during transportation has a significant impact on its shelf life.

As a result, this issue would be included in a food safety management system.

The heating instructions on the package say "do not microwave this food" but the consumer microwaves and then eats the food is not a food safety issue.

As a result, this issue would not be included in a food safety management system. Hence, this is the answer to the question.

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You are studying ABO blood groups, and know that 1% of the population has genotype IB1B and 42.25% of the population has Type O blood. What is the expected frequency of blood type A? (Assume H-W equilibrium) Hint: the question is about the expected frequency of phenotype blood type A or, what percentage of the population has type A blood? A.25%
B. 51.5%
C. 6.5%
D. 1% E.39%

Answers

The expected frequency of phenotype blood type A or, what percentage of the population has type A blood is A.25%.

ABO blood groups follow the principle of codominance. Individuals can have A and B, or O blood groups, according to the expression of two co-dominant alleles. The frequency of individuals with blood type O is 42.25% in the population. The genotype frequency of IB1B is 1%. Since the A and B alleles are codominant, the frequency of the IA1IA1 and IA1IB1 genotypes would have to be added together to get the expected frequency of blood type A: IA1IA1 + IA1IB1.

The Hardy-Weinberg equilibrium formula is p^2+2pq+q^2 = 1 where p and q represent allele frequencies and p+q = 1. Because we are solving for p^2 and 2pq, we can use the following formula: p^2 = IA1IA1 and 2pq = IA1IB1.

Substituting the values, we get 2pq = 2(0.21)(0.79) = 0.33.

Therefore, the frequency of IA1IA1 = p^2 = (0.21)^2 = 0.0441.

Adding the two frequencies together, we get:0.0441 + 0.33 = 0.3741.

Since blood types A and B are codominant, the frequency of B is also expected to be 37.41%.

Subtracting both A and B blood type frequencies from the total gives: 1 - 0.3741 - 0.4225 = 0.2034 or 20.34%, which is the expected frequency of blood type O.

Therefore, the expected frequency of blood type A is 25% (0.25). The correct answer is A. 25%.

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The last two years of global pandemic made many people aware of how important our immune system is to defend us from viral diseases. List at least two defense mechanisms (either innate or adaptive) which protect us from viruses, including SARS-CoV-2.

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The last two years of the global pandemic have made people aware of the importance of their immune system to defend against viral diseases. The immune system has two defense mechanisms, innate and adaptive, that protect us from viruses, including SARS-CoV-2. The following are the two defense mechanisms of the immune system:1. Innate Immune System The innate immune system is the first line of defense against viral infections.

It is a quick and nonspecific immune response that provides immediate defense against infections. When a virus infects the body, the innate immune system releases molecules called cytokines that help to recruit immune cells, such as neutrophils, dendritic cells, and macrophages, to the site of infection. These cells engulf and destroy the virus and infected cells.2. Adaptive Immune System The adaptive immune system provides long-term defense against viruses.

It is a specific immune response that is tailored to the specific virus. The adaptive immune system produces antibodies that recognize and bind to the virus, preventing it from infecting cells. It also activates immune cells called T cells and B cells, which destroy the virus and infected cells. The adaptive immune system also has memory cells that can recognize and respond quickly to the virus if it enters the body again.

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Explain the roles of key regulatory agencies within the United
States in the safe release of bioengineered organisms in the
environment and in regulating food and food additives produced
using biotech

Answers

The key regulatory agencies in the United States for the safe release of bioengineered organisms and regulation of biotech food and additives are the EPA, USDA, and FDA.

The key regulatory agencies within the United States that play important roles in the safe release of bioengineered organisms in the environment and in regulating food and food additives produced using biotech include the U.S. Environmental Protection Agency (EPA), the U.S. Department of Agriculture (USDA), and the Food and Drug Administration (FDA).

The U.S. Environmental Protection Agency (EPA) is responsible for regulating bioengineered organisms that are intended to be released into the environment. The EPA evaluates the potential risks associated with these organisms and assesses their potential impact on ecosystems and human health. They ensure that appropriate measures are in place to minimize any potential adverse effects and to protect the environment.

The U.S. Department of Agriculture (USDA) plays a role in regulating bioengineered crops and organisms. The USDA's Animal and Plant Health Inspection Service (APHIS) is responsible for assessing the potential risks and impacts of genetically modified crops and organisms on agriculture and the environment. They oversee the permitting process for field trials and commercialization of genetically modified crops.

The Food and Drug Administration (FDA) is responsible for regulating food and food additives produced using biotechnology. The FDA ensures that these products are safe for consumption and accurately labeled. They evaluate the safety and nutritional profile of genetically modified crops, as well as the safety of food additives derived from biotech processes.

These regulatory agencies work together to establish and enforce regulations and guidelines to ensure the safe release of bioengineered organisms and the regulation of biotech-derived food and food additives in the United States. Their collective efforts aim to protect the environment, safeguard public health, and provide consumers with accurate information about the products they consume.

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