Photosynthesis does not occur regularly in polar regions during winter, so the statement is False.
The products of photosynthesis are actually glucose (or other carbohydrates) and oxygen (O2), not carbon dioxide (CO2) and water (H2O). So the statement is False.
The statement that upwelling of deep, cold, nutrient-rich waters is the only way for nutrients to reach coastal surface waters is False. While upwelling is an important mechanism for nutrient supply in coastal areas, there are other processes such as river runoff and atmospheric deposition that also contribute to the nutrient input in coastal surface waters.
High-pressure systems in the Southern Hemisphere rotate counterclockwise, opposite to the rotation in the Northern Hemisphere. So the statement is False.
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all about ELISA. 6. Why is the color reagent necessary? What is it reacting with?
7. What does a change in color indicate?
9. Does an ELISA look for the Coronavirus genetic material (evidence of viral particle) or a person’s antibodies to the Coronavirus? 8. Would an ELISA be used to diagnose a current infection or test to see if a person has been previously exposed, say a month ago?
ELISA (enzyme-linked immunosorbent assay) is an antibody-based diagnostic assay that is widely used to detect and quantify analytes such as proteins, peptides, antibodies, and hormones.
6. The color reagent is necessary to visualize the binding of the primary antibody to the antigen of interest, which indicates whether the analyte is present or not.
The color reagent reacts with an enzyme-labeled secondary antibody, which binds to the primary antibody in the previous step and is used to generate a signal.
The color reagent changes color when it interacts with the enzyme that is linked to the secondary antibody.
7. A change in color indicates the presence of the analyte of interest.
If the analyte is present, the color of the sample changes due to the enzyme-labeled secondary antibody's reaction with the color reagent.
8. An ELISA would be used to test if a person has been previously exposed to the virus, but it would not be used to diagnose a current infection.
9. ELISA looks for the antibodies that a person’s body produces to fight the Coronavirus and not the viral particle itself.
The ELISA test detects antibodies against the SARS-CoV-2 virus, which is the virus that causes COVID-19.
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Please help and explain thank you
- How does an increase in co2 concentration in the blood affect the pH of cerebrospinal fluid?
- what determines whether o2 and co2 undergo net diffusion into or our of capillaries, explain.
An increase in CO2 concentration in the blood leads to a decrease in the pH of cerebrospinal fluid. The concentration of carbon dioxide (CO2) in the blood is an important factor that influences the pH of (CSF).
When CO2 levels increase in the blood, such as during exercise or due to respiratory issues, it diffuses across the blood-brain barrier into the CSF. In the CSF, carbonic anhydrase, an enzyme present in the choroid plexus, catalyzes the conversion of CO2 and water into carbonic acid (H2CO3). Carbonic acid then dissociates into bicarbonate ions (HCO3-) and hydrogen ions (H+). The increase in hydrogen ions in the CSF leads to a decrease in pH, resulting in acidosis.
Regarding the net diffusion of oxygen (O2) and carbon dioxide (CO2) into or out of capillaries, several factors come into play. The process is primarily determined by concentration gradients, partial pressures, and diffusion distances. In the lungs, where oxygen is taken up and carbon dioxide is released, the partial pressure of O2 is higher in internal respiration the alveoli than in the capillaries, creating a concentration gradient that drives the diffusion of O2 into the bloodstream. Simultaneously, the partial pressure of CO2 is higher in the capillaries than in the alveoli, favoring the diffusion of CO2 out of the bloodstream.
In systemic capillaries, where oxygen is released and carbon dioxide is taken up, the partial pressure gradients are reversed. The partial pressure of O2 is higher in the capillaries than in the surrounding tissues, causing O2 to diffuse out of the bloodstream and into the cells. Conversely, the partial pressure of CO2 is higher in the tissues than in the capillaries, facilitating the diffusion of CO2 from the cells into the bloodstream.
Other factors, such as the solubility of gases and the surface area available for diffusion, also contribute to the net diffusion of O2 and CO2 across capillaries. Overall, the direction of net diffusion depends on the concentration gradients and partial pressures of the gases involved, ensuring the exchange of O2 and CO2 to meet the metabolic needs of the body.
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44 00:50:04 Why is it necessary to include some carbohydrates in the diet? Multiple Choice Carbohydrates are considered a "complete" nutrient Some carbohydrates contain essential fatty acids Carbohydr
Carbohydrates are necessary to include in the diet because they serve as a major source of energy for the body. Option A is the answer.
Carbohydrates are one of the three macronutrients, along with fats and proteins, that provide energy to the body. When consumed, carbohydrates are broken down into glucose, which is used by cells as fuel. The brain, in particular, relies heavily on glucose for its energy needs. Additionally, carbohydrates play a role in supporting proper digestive function, providing dietary fiber for bowel regularity, and promoting satiety.
While carbohydrates are not considered a "complete" nutrient like proteins, they are essential for overall energy balance and maintaining optimal health. Option A is the answer.
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The main cause of the relative refractory period is:
a. Hyperpolarization of the cell membrane at the end of an action potential.
b. The opening of voltage-gated sodium channels.
c. The activity of the sodium-potassium pump.
d. None of the above causes the relative refractory period.
The main cause of the relative refractory period is hyperpolarization of the cell membrane at the end of an action potential. The correct option is A.
The relative refractory period is a section of time following the absolute refractory period, which is the brief period when a neuron can't generate another action potential because its voltage-gated sodium channels are inactive.The relative refractory period is described as the stage in which a neuron can generate an action potential, but only if the stimulus is powerful enough. This is due to the hyperpolarization of the cell membrane that occurs after an action potential. It happens because potassium channels are still open and chloride channels are closed. This causes the membrane potential to become more negative, making it more difficult for the neuron to generate an action potential.
The relative refractory period, on the other hand, is critical because it allows for the control of the frequency and pattern of action potentials that are sent down axons. The sodium-potassium pump is essential for restoring the resting membrane potential following an action potential, but it is not directly responsible for the relative refractory period. Therefore, the correct option is a.
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Which of the following term does NOT involve multiple loci. a. Complex traits b. Independent assortment c. Haplotype Recombinant d. Random segregation Trisomy 21. Robertsonian translocation and PKU syndrome are three human phenotypes due to DNA changes in different scales. Which of the following shows the order of DNA alterations, in large to small scales, that cause these phenotypes? a. Robertsonian translocation > trisomy 21 PKU syndrome
b. None of other answers is correct.
c. trisomy 21 > Robertsonian translocation > PKU syndrome d. PKU syndrome > trisomy 21 > Robertsonian translocation
e. PKU syndrome > Robertsonian translocation trisomy 21
1. c. Haplotype Recombinant.
2. c. trisomy 21 > Robertsonian translocation > PKU syndrome.
1. The term that does not involve multiple loci is c. Haplotype Recombinant. Haplotype refers to a set of genetic variations on a single chromosome, while recombinant refers to the reshuffling of genetic material during meiosis. It does not inherently involve multiple loci, but rather the arrangement of alleles at a single locus.
2. The correct order of DNA alterations, in large to small scales, that cause the phenotypes mentioned is c. trisomy 21 > Robertsonian translocation > PKU syndrome. Trisomy 21 refers to the presence of an extra copy of chromosome 21, resulting in Down syndrome, which is a large-scale alteration involving a whole chromosome. Robertsonian translocation involves the fusion of two acrocentric chromosomes, often resulting in rearrangements and genetic disorders. Phenylketonuria (PKU) syndrome, on the other hand, is caused by alterations in a single gene, resulting in the inability to metabolize phenylalanine. Therefore, the order from large-scale to small-scale DNA alterations is trisomy 21, Robertsonian translocation, and PKU syndrome.
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The incidence of prostate cancer in Canada in 2016 is 114.7 per 100,000 men and the prevalence of the same disease is 1100 per 100,000 men.
Estimate the average duration in years of prostate cancer.
Please select one answer:
a.It cannot be calculated.
b.0.1 years
c.10.4 years
d.9.6 years
The prevalence of a disease is the number of people who have the disease in a particular population at a certain time, and the incidence of a disease is the number of new cases of the disease in a population during a specific period of time.
Therefore, the prevalence of prostate cancer in Canada in 2016 was 1100 per 100,000 men and the incidence of prostate cancer was 114.7 per 100,000 men.Now, we have to estimate the average duration in years of prostate cancer. To achieve this, we can divide the prevalence by the incidence.
The answer will be:Average duration = Prevalence / Incidence= 1100/114.7≈ 9.6 years.
Therefore, the correct answer is d. 9.6 years.
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1) Which gene was duplicated in human that increased brain growth? 2) Most if not all primates have an insula a) True b) False 3) Which gene was mutated to increase cortex complexity?
1) The gene duplicated in humans that increased brain growth is the gene called ARHGAP11B.
2) False, most primates do have an insula.
3) The gene mutated to increase cortex complexity is the gene called FOXP2.
1) The ARHGAP11B gene is a human-specific gene that is believed to have played a crucial role in the expansion of the human brain. This gene is thought to be involved in regulating the proliferation of neural stem cells, leading to increased brain size and complexity in humans.
2) False, most primates do have an insula. The insula is a region of the brain that is involved in various functions such as sensory perception, motor control, emotions, and self-awareness. It is found in many primate species, including humans.
3) The FOXP2 gene is known for its role in language development. It has been found that mutations in the FOXP2 gene can lead to impaired speech and language abilities. Studies have suggested that changes in the FOXP2 gene played a role in the evolution of increased cortical complexity, particularly in the areas of the brain associated with language and speech production.
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Explain following statement with a proper example: Pyruvate carboxylase can act as Kinase, Lyase, Transferase and Ligase. 5
Pyruvate carboxylase is an enzyme that exhibits multiple catalytic activities, including kinase, lyase, transferase, and ligase.
Enzymes are proteins that catalyze specific chemical reactions in living organisms. Pyruvate carboxylase is an enzyme involved in various metabolic pathways, including gluconeogenesis, fatty acid synthesis, and amino acid metabolism. It plays a crucial role in converting pyruvate, a product of glycolysis, into oxaloacetate, which serves as a key intermediate in these metabolic processes.
Pyruvate carboxylase can act as a kinase by transferring a phosphate group from ATP to pyruvate, resulting in the formation of phosphoenolpyruvate. As a lyase, it catalyzes the cleavage of a carbon-carbon bond in pyruvate, generating carbon dioxide and acetyl-CoA. Additionally, it can function as a transferase by transferring a functional group, such as a carboxyl group, from one molecule to another. Lastly, as a ligase, it can catalyze the joining of two molecules using energy from ATP hydrolysis.
Overall, the multifunctionality of pyruvate carboxylase allows it to participate in various metabolic pathways and contribute to the synthesis and breakdown of important cellular components.
The ability of pyruvate carboxylase to exhibit different catalytic activities highlights the versatility of enzymes in carrying out diverse biochemical reactions in living organisms. The specific activity of pyruvate carboxylase depends on the cellular context and the metabolic needs of the organism. Understanding the different functions of enzymes like pyruvate carboxylase provides insights into the complex interplay of metabolic pathways and their regulation.
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7.
What are they key differences between Cytotoxic T cells and Helper
T cells?
Cytotoxic T-cells and Helper T-cells are different types of cells that have different functions in the immune system of the body.
The following are the key differences between Cytotoxic T-cells and Helper T-cells:
1. Function: Cytotoxic T-cells are responsible for recognizing and destroying infected and cancerous cells by producing cytotoxins. Helper T-cells help other cells of the immune system to perform their functions.
2. Receptors: Cytotoxic T-cells have CD8 receptors that bind to MHC class I molecules present on the surface of infected or cancerous cells, whereas Helper T-cells have CD4 receptors that bind to MHC class II molecules on the surface of antigen-presenting cells.
3. Antigens: Cytotoxic T-cells recognize antigens presented by MHC class I molecules, whereas Helper T-cells recognize antigens presented by MHC class II molecules.
4. Action: Cytotoxic T-cells directly attack the infected or cancerous cells, whereas Helper T-cells secrete cytokines that activate other immune cells to perform their functions.
5. Target: Cytotoxic T-cells target and destroy cells infected with viruses and some bacteria, as well as cancer cells. Helper T-cells target cells presenting foreign antigens, such as bacteria or viruses.In conclusion, Cytotoxic T-cells and Helper T-cells have different functions in the immune system, and they recognize different types of antigens. Their receptors and target cells are also different.
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You examine sperm removed from the lumen of the epididymis. What
will you find?
a. Sperm undergoing meiotic cell divisions
b. Sperm undergoing mitotic cell divisions
c. Sperm in which cholesterol is b
Examining sperm removed from the lumen of the epididymis would reveal sperm in which cholesterol is present.
The epididymis is a coiled tube located in the male reproductive system, where sperm cells mature and acquire certain characteristics necessary for successful fertilization. One of these characteristics is the incorporation of cholesterol into the sperm membrane. Cholesterol plays a crucial role in maintaining the integrity and fluidity of the sperm cell membrane.
When examining sperm removed from the lumen of the epididymis, one would find sperm cells that have undergone maturation processes, including the incorporation of cholesterol into their membranes. This cholesterol helps to stabilize the structure of the sperm cell, ensuring that it maintains its viability and functional abilities during the journey through the female reproductive tract.
Therefore, the correct answer is c. Sperm in which cholesterol is present. The presence of cholesterol in the sperm membranes is a characteristic feature of mature sperm cells that have completed their development within the epididymis.
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Your supervisor also required you to run a 5-day BOD test using some of the activated sludge as a biomass inoculum in addition to the microbes in the raw influent. To perform this experiment in one bottle you prepare a mixture of 2 mL of raw influent, 1 ml of activated sludge, and 297 mL of diluent water. In a second control bottle you add 1 mL of activated sludge and 299 mL of diluent water. At the beginning of the test the DO level in both bottles in 8 mg/L. After incubation for 5 days at 20C in the dark, the DO level in the bottle with the raw influent is 5.0 mg/L and the DO level in the control bottle is 7.7 mg/L. What is the 5-day BOD for the influent during this experiment?
The 5-day BOD for the influent in this experiment is determined by the difference in DO levels between the raw influent bottle and the control bottle over the 5-day incubation period.
In the raw influent bottle, the DO level decreased from 8 mg/L to 5.0 mg/L over the 5-day period, indicating that 3 mg/L of oxygen was consumed by the microbes. In the control bottle, the DO level decreased from 8 mg/L to 7.7 mg/L over the 5-day period, indicating that only 0.3 mg/L of oxygen was consumed by the microbes.
This difference in oxygen consumption between the raw influent and the control bottle is due to the presence of biodegradable organic matter in the raw influent that was not present in the control bottle. The 5-day BOD for the influent can be calculated as follows:
BOD = [(initial DO - final DO in raw influent bottle) - (initial DO - final DO in control bottle)] x Dilution FactorBOD = [(8 mg/L - 5.0 mg/L) - (8 mg/L - 7.7 mg/L)] x (300/2)BOD
= (3.0 mg/L - 0.3 mg/L) x 150BOD = 405 mg/L.
Therefore, the 5-day BOD for the influent in this experiment is 405 mg/L.
From the above solution, we can conclude that the 5-day BOD for the influent in this experiment is 405 mg/L.
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A Lactobacillus strain is growing in milk. At 5 hours the cell concentration is 5 x 10 CFU/ml whereas at 10 hours the cell concentration is 4 x 108 CFU/ml. Assuming that the cells are growing exponentially during this period, calculate the maximum specific growth rate (max) (30 marks)
To calculate the maximum specific growth rate, we can use the following formula:
[tex]μmax = ln(N2/N1)/t2-t1[/tex]
where N1 is the cell concentration at time 1, N2 is the cell concentration at time 2, t1 is the time at time 1, and t2 is the time at time 2.
Using the given data, we can plug in the values:
[tex]μmax = ln(4 x 108/5 x 105)/(10-5)μ[/tex]
[tex]max = ln(8 x 103)/5μmax[/tex]
[tex]= 5.66 x 10-4 per hour or 0.566 per day[/tex]
the maximum specific growth rate is [tex]5.66 x 10-4[/tex] per hour or 0.566 per day.
Now, we can substitute these values into the equation:
[tex]μmax = 9.08 / 5 ≈ 1.82 CFU/ml/hour[/tex]
the maximum specific growth rate (μmax) of the Lactobacillus strain is approximately [tex]1.82 CFU/ml/hour[/tex].
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You are going to perform a Gram staining with two bacterial strains, Pseudomonas aeruginosa and Enterococcus faecium. Describe all necessary steps of Gram staining procedure including the biochemical principles behind each staining and de-staining steps. Discuss the expected colour and shape
Gram staining is an essential technique used to distinguish between bacterial cell walls that vary in chemical composition.
The technique relies on the crystal violet stain that distinguishes between gram-negative and gram-positive bacteria. The purpose of this study is to provide a comprehensive explanation of the steps required to conduct Gram staining for Pseudomonas aeruginosa and Enterococcus faecium. To accomplish the task, the following procedures were followed: Step 1:Preparing bacterial smear First, a bacterial smear is made by putting a drop of water on a glass slide and sterilizing it by a Bunsen burner. With a sterile loop, a small amount of bacterial culture was put in the drop of water on the glass slide and blended well.
Afterward, it was allowed to dry in the air. Step 2: Fixing the smear The smear was then fixed by heat fixation. It was passed through the Bunsen burner three times and allowed to cool for a while. This procedure enables the bacteria to stick to the glass slide.
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Then, analyze the common practice at home/college that you think is a problem that fails the recycling effort. You can either interview your parents or ask your colleagues (but please, consider social distancing and safety). -Type of wastes that have been accumulated in 2-3 days in your bin. -Contamination for each waste should be highlighted IN DETAIL.
- Common practice at home/college on recycling practices should be elaborated well.
- Factors that you think hinders the recycling activity. -Suggestion for improvement. -Reflection on the outputs in relation to what have you obtained in your class.
Common practices that hinder recycling efforts include improper sorting of recyclables, contamination of recyclable materials with non-recyclables such as food residue or plastic bags, and lack of awareness about recycling guidelines.
Factors that contribute to these practices include a lack of education, inadequate infrastructure, inconsistent recycling programs, and a lack of motivation. Suggestions for improvement include education and awareness campaigns, clear labeling on recycling bins, improved infrastructure, and incentives for proper recycling.
Class-related outputs can play a role in raising awareness and finding solutions to recycling challenges, promoting sustainable habits, and contributing to a more environmentally friendly society.
Types of wastes that may accumulate in bins: The specific types of wastes that accumulate will vary depending on the location and individual habits. However, common items found in household or college bins could include food waste, plastic packaging, paper, glass bottles, aluminum cans, and other recyclable materials.
Contamination: Contamination occurs when non-recyclable materials are mixed with recyclables, making them unsuitable for recycling. Examples of contamination include food residues on containers, liquids in bottles, plastic bags mixed with recyclables, and non-recyclable items like Styrofoam or certain types of plastic.
Common recycling practices: Common practices at home or college that may hinder recycling efforts include lack of awareness about what can and cannot be recycled, improper sorting of recyclables, and not rinsing containers to remove food residue.
Factors hindering recycling activity:
a) Lack of education and awareness about recycling guidelines and the importance of proper recycling.
b) Inadequate infrastructure and accessibility to recycling facilities.
c) Inconsistency in recycling programs and collection systems.
d) Convenience and lack of motivation to recycle properly.
Suggestions for improvement:
a) Education and awareness campaigns to inform individuals about recycling guidelines and the impact of their actions.
b) Clear labeling and signage on recycling bins to facilitate proper sorting.
c) Improvement in recycling infrastructure and accessibility to increase convenience and participation.
d) Incentives and rewards for proper recycling practices to motivate individuals.
Reflection on class-related outputs: Although I don't have access to personal class-related experiences, in general, class discussions or projects on recycling can help raise awareness, promote understanding of proper recycling practices, and explore solutions to recycling challenges.
By analyzing waste accumulation and contamination, discussing factors hindering recycling, and providing suggestions for improvement, students can contribute to a more sustainable environment and encourage positive recycling habits within their communities.
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Disorders of the Ear
Describe otitis media and its cause, pathophysiology, and
signs
Describe the pathophysiology and signs of otosclerosis and of
Meniere’s syndrome
Explain how permanent hearing l
Otitis Media: Cause: Otitis media refers to inflammation or infection of the middle ear. It is commonly caused by a bacterial or viral infection that spreads from the upper respiratory tract or Eustachian tube dysfunction.
Pathophysiology: In otitis media, the Eustachian tube, which connects the middle ear to the back of the throat, becomes blocked or dysfunctional. This leads to the accumulation of fluid in the middle ear, providing a suitable environment for bacteria or viruses to grow and cause infection. The inflammation and fluid buildup can result in pain, pressure, and impaired hearing.
Signs: Common signs of otitis media include ear pain, hearing loss, feeling of fullness or pressure in the ear, fever, fluid draining from the ear, and sometimes redness or swelling of the ear.
Otosclerosis: Otosclerosis is a condition characterized by abnormal bone growth in the middle ear, specifically around the stapes bone, which impairs its ability to transmit sound waves to the inner ear. This abnormal bone growth restricts the movement of the stapes, resulting in conductive hearing loss.
Signs: Signs of otosclerosis include progressive hearing loss, tinnitus (ringing in the ears), dizziness or imbalance, and sometimes a family history of the condition.
Meniere's Syndrome: Meniere's syndrome is a disorder of the inner ear that affects balance and hearing. It is believed to be caused by an abnormal accumulation of fluid in the inner ear, known as endolymphatic hydrops. The exact cause of this fluid buildup is not fully understood, but it may be related to factors such as fluid regulation disturbances, allergies, or autoimmune reactions.
Signs: Meniere's syndrome is characterized by episodes of vertigo (intense spinning sensation), fluctuating hearing loss (usually in one ear), tinnitus, and a feeling of fullness or pressure in the affected ear. These episodes can last for several hours to a whole day and may be accompanied by nausea and vomiting.
Permanent Hearing Loss:Permanent hearing loss can occur due to various factors, including damage to the hair cells in the inner ear, damage to the auditory nerve, or structural abnormalities in the ear.
Exposure to loud noises, certain medications, aging, infections, genetic factors, and other medical conditions can contribute to permanent hearing loss.
Once the delicate structures involved in hearing are damaged or impaired, they cannot be regenerated or repaired, leading to permanent hearing loss. Treatment options for permanent hearing loss often involve the use of hearing aids or cochlear implants to amplify sound and improve hearing.
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(0)
#1 Mutations were mentioned only briefly in lecture. Read about it in your text in Chapter 10, and briefly explain the following kinds of mutations.
Base/Letter Substitution: ____ ____
Base/Letter Addition: ____ ____
Nucleotide/Codon Deletion: ____ ____
#2 Explain why a mutation of Base/Letter Substitution or Addition would have a larger effect on the resulting protein than a mutation of Nucleotide/Codon Deletion or Insertion. ____ ____
Nucleotide/Codon Insertion: ____ ____
Nucleotide/Codon Jumping: ____ ____
Base/Letter Substitution: A single nucleotide base is replaced by another base, resulting in a change in the corresponding amino acid during protein synthesis.
Base/Letter Addition: An extra nucleotide base is inserted into the DNA sequence, leading to a shift in the reading frame and a different sequence of amino acids in the resulting protein.
Nucleotide/Codon Deletion: One or more nucleotide bases are removed from the DNA mutation, causing a shift in the reading frame and a different amino acid sequence in the resulting protein.
Mutation Effect: Base/Letter Substitution or Addition mutations have a larger impact on the resulting protein because they alter the reading frame and can introduce a completely different sequence of amino acids. In contrast, Nucleotide/Codon Deletion or Insertion mutations can cause a frame shift but may not completely change the sequence of amino acids.
Base/Letter Substitution: In this type of mutation, a single nucleotide base is substituted with another base. The altered DNA sequence will code for a different amino acid during protein synthesis, potentially leading to a different protein structure and function. The effect of this mutation depends on the specific substitution and its impact on the resulting amino acid sequence.
Base/Letter Addition: This mutation involves the insertion of an extra nucleotide base into the DNA sequence. As a result, the reading frame shifts, and the subsequent codons are read differently during protein synthesis. This alteration in the reading frame can significantly change the amino acid sequence, potentially leading to a completely different protein structure and function.
Nucleotide/Codon Deletion: In this mutation, one or more nucleotide bases are deleted from the DNA sequence. This causes a shift in the reading frame, leading to a different grouping of codons during protein synthesis. As a result, the amino acid sequence is altered, which can affect the structure and function of the resulting protein.
Mutation Effect: Base/Letter Substitution or Addition mutations have a larger impact on the resulting protein because they can introduce significant changes in the amino acid sequence. These mutations can disrupt the reading frame and potentially produce a completely different protein sequence. In contrast, Nucleotide/Codon Deletion or Insertion mutations may cause a frame shift, but the impact on the resulting protein can vary depending on the specific sequence affected. The magnitude of the effect also depends on the position of the mutation within the gene and the functional importance of the affected region.
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For E. coli O157:H7 Enterohemorrhagic E. coli answer the
following questions: What is this bacteria’s morphology/type? How
is it transmitted to people? List and explain 2 virulence factors.
Briefly
E. coli O157:H7 is a Gram-negative bacterium, meaning it has a thin peptidoglycan layer and an outer membrane. Morphologically, it appears as a rod-shaped bacterium under a microscope.
The primary mode of transmission of E. coli O157:H7 to humans is through the consumption of contaminated food or water. It is commonly associated with undercooked ground beef, raw milk, contaminated vegetables, and contaminated water sources. Person-to-person transmission can also occur, especially in settings with poor hygiene practices.
E. coli O157:H7 possesses various virulence factors that contribute to its pathogenicity. Two important virulence factors are:
Shiga toxins: E. coli O157:H7 produces Shiga toxins, also known as verotoxins. These toxins inhibit protein synthesis in host cells, leading to cell damage and tissue injury. They are responsible for the development of severe symptoms such as bloody diarrhea and can cause complications like hemolytic uremic syndrome (HUS).
Adhesins: E. coli O157:H7 possesses specific adhesins that enable it to attach to the intestinal epithelial cells, allowing colonization and persistence in the gut. This adhesion capability enhances its ability to cause infection and evade the host's immune response.
These virulence factors contribute to the pathogenicity of E. coli O157:H7 and are responsible for the severity of the associated diseases.
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What enzyme is most responsible for the conversion of
chylomicrons into chylomicron remnants and of very low density
lipoproteins into IDL particles?
The enzyme that is most o know more vio for the conversion of chylomicrons into chylomicron remnants and very low-density lipoproteins (VLDLs) into intermediate-density lipoprotein (IDL) particles is lipoprotein lipase (LPL).
What is lipoprotein lipase (LPL)?Lipoprotein lipase is an enzyme that catalyzes the breakdown of triglycerides in circulating chylomicrons and VLDLs, releasing fatty acids that can be absorbed and used as energy by cells.Lipoprotein lipase is found on the surface of cells that line blood vessels and is produced by many tissues, including muscle and adipose tissue. Insulin stimulates the synthesis of lipoprotein lipase, which increases the uptake of fatty acids by adipose tissue, reducing circulating lipid levels and increasing fat storage.
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Alternative splicing and overlapping genes enable organisms to O a. reduce the fitness impact of parasitic DNA. O b. ensure that functionally related genes evolve together. O c. increase the range of protein product they produce without expanding genome size. O d. produce gene duplicates with novel function. O e. regulate gene expression at the level of protein translation.
Alternative splicing and overlapping genes enable organisms to increase the range of protein products they produce without expanding genome size. Correct answer is option C
Alternative splicing is a mechanism by which different combinations of exons are included or excluded during mRNA processing, resulting in multiple protein isoforms from a single gene.
This allows for increased diversity and functional complexity without the need for a larger genome. Overlapping genes, on the other hand, refer to genes that share a common stretch of DNA, enabling the production of different proteins from the same region.
Both alternative splicing and overlapping genes contribute to expanding the proteome without significantly increasing the genome size. Correct answer is option C
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Which of the following is a property of intraepithelial lymphocytes?
O They include gamma-delta T cells
O They are not activated
O They are CD4+ T cells
O They express the integrin AeB7
O They express receptors with a broad range of specificities
The following is a property of intraepithelial lymphocytes is they include gamma-delta T cells. The correct answer is a.
Intraepithelial lymphocytes (IELs) are a specialized population of lymphocytes found within the epithelial layer of various tissues, particularly the mucosal surfaces of the gastrointestinal tract. One of the distinguishing features of IELs is that they include gamma-delta T cells.
Gamma-delta T cells are a subset of T cells that possess a unique T-cell receptor (TCR) composed of gamma and delta chains. Unlike conventional alpha-beta T cells, which recognize peptide antigens presented by major histocompatibility complex (MHC) molecules, gamma-delta T cells can recognize a wide range of antigens, including microbial products and stress-induced molecules, without the need for MHC presentation.
So, the property of intraepithelial lymphocytes (IELs) being highlighted in the given options is that they include gamma-delta T cells.
Therefore, the correct answer is a.
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G proteins A. bind GTP. B. dephosphrylate ITAMs. c. are transcription factors. D. downmodulate immune responses. E. are adhesion molecules.
G proteins bind GTP.
The correct answer to the question is option A.
The G protein hydrolyzes the bound GTP to GDP, inactivating itself and allowing the cycle to begin again.
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Rates of calcification in the Corallinales are highest when pH
is a) low b) neutral c) high
The rates of calcification in the Corallinales are highest when pH is high. The Corallinales is an order of red algae.
They are found in marine environments worldwide, including the deep sea and the intertidal zone. They have a calcified skeleton that makes them important reef-building organisms, and they are frequently found in coral reefs. These organisms are also used as food in some cultures, and they are sometimes used in traditional medicine.
The Corallinales has a calcified skeleton that makes them important reef-building organisms. Calcification is the process by which organisms such as Corallinales secrete calcium carbonate to form a hard, protective structure around themselves.
The rates of calcification in the Corallinales are influenced by a variety of factors, including pH. Research has shown that the rates of calcification in the Corallinales are highest when pH is high. When the pH is low, the Corallinales experience a decrease in calcification rates, which can have negative consequences for their survival and the ecosystem they are a part of.In conclusion, the rates of calcification in the Corallinales are highest when pH is high.
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What is the definition of tissue? What is the definition of organ? Give an example? What is the definition of system? Give an example? Look at these slides under 4X, 10X and 40X. There are 4 different types of Human tissue: 1. Epithelial tissue Have different shapes: they are either columnar, cuboidal, or squamous (look at the models). Observe the slides of human skin, lung, kidney and intestine Look at the model of human skin
What are the functions of epithelial tissue? 2. Nervous tissue are different types: but the ones that you should recognize are the neurons, which have: axons, dendrite, and cell body. Observe the slides and model of the neurons. What are the functions of nervous tissue? 3. Connective tissue are different than the rest of the tissues. They either have living cells or they are just a structure like the bone Observe the slide of the bone, you should be able to see: osteocytes, canaliculi. Haversian canals, Yolkmann's canal, and matrix. Look at the slide of skin and under epidermis and see the connective tissue (dermis layer). Can you find the adipocytes in the slide of skin? How many different tissue can you identify in the skin slide? Identify, epithelial tissue, adipocytes, connective tissue, epidermis and dermis.
Look at the slide of blood and models of blood. The red blood cells are called erythrocytes. White blood cells are referred to as leukocytes. Identify neutrophils, basophils, cosinophils monocytes, and lymphocytes. Also look at the models of neutrophils, basophils, eosinophils and lymphocytes. What are the functions of connective tissue?
Look at this slide under 10X and 40X. 4. Muscular tissue There are three different types of muscles:
A. cardiac muscle: make sure you see striation and intercalated discs. The cardiac muscles are connected to each other by these discs. B. smooth muscles are NOT striated. They are our involuntary muscles. Where do you find smooth muscles in our body?
C. Skeletal muscle which are striated. They are found in our voluntary muscles. One cell can have several nuclei. Observe the slide of the three different types of muscle. Be able to tell them apart.
What are the functions of muscular tissue?
Look at the models of skeletal tissue and smooth muscle tissue. There is not a model of cardiac muscle. Look at the slide of intestine and locate the smooth muscle? How many different types tissue can you identify in the slide of intestine? 28
Tissue is defined as a group of cells that have similar structures and functions. An organ is a structure made up of different types of tissues that work together to perform a specific function in the body.
A system is a group of organs that work together to perform a specific function in the body.
Examples of tissues, organs, and systems:
Tissue: Epithelial tissue - Function: Protects the body's surfaces, absorbs nutrients, and secretes substances. Example: Skin tissue, Lung tissue, Kidney tissue, and Intestinal tissue.
Tissue: Nervous tissue - Function: Communicates messages throughout the body. Example: Neurons.
Tissue: Connective tissue - Function: Provides support and structure to the body. Example: Bone, skin (dermis layer), and Adipose tissue.
Tissue: Muscular tissue - Function: Movement of the body. Example: Cardiac muscle, Smooth muscle (found in organs and blood vessels), and Skeletal muscle.
Epithelial tissue functions:
- Protecting the body from external factors such as microorganisms, chemical substances, and mechanical stress.
- Absorbing nutrients from the gastrointestinal tract and reabsorbing substances from the kidneys.
- Secreting hormones, enzymes, and mucus.
Nervous tissue functions:
- Responding to stimuli.
- Transmitting and processing information.
- Controlling and coordinating body functions.
Connective tissue functions:
- Supporting and protecting body tissues.
- Providing structural support to the body.
- Connecting body parts.
Muscular tissue functions:
- Generating body heat.
- Moving the body and body parts.
- Pumping blood through the heart.
In the slide of the intestine, three types of tissues can be identified - epithelial tissue, connective tissue, and smooth muscle tissue.
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Describe the flow of genetic information (DNA → mRNA → polypeptide chains → native proteins) and the levels at which this is regulated
The flow of genetic information refers to the transfer of genetic information from DNA to protein. This process is called gene expression and consists of two main steps: transcription and translation.
Transcription involves the conversion of DNA into RNA (mRNA). The process begins when RNA polymerase, an enzyme, binds to a specific sequence on the DNA, called the promoter region. Once RNA polymerase has bound to the DNA, it starts to unzip the DNA double helix and builds an RNA molecule that is complementary to one of the DNA strands.
When the RNA polymerase reaches the end of the DNA, the mRNA molecule is complete. Translation is the process by which ribosomes build polypeptide chains from the mRNA sequence.
Translation occurs in three stages: initiation, elongation, and termination. During initiation, the ribosome binds to the mRNA molecule, and the first amino acid is brought in by a tRNA molecule.
During elongation, the ribosome reads the mRNA sequence, adds the next amino acid, and moves down the mRNA molecule until a stop codon is reached. During termination, the ribosome falls apart, and the newly synthesized protein is released.Protein synthesis is regulated at different levels. DNA transcription is regulated by DNA sequences that control when and where genes are expressed.
Post-transcriptional regulation involves RNA processing, such as splicing, editing, and degradation, which can affect the mRNA's stability, transport, and translation. Translation regulation involves the control of protein synthesis by factors such as mRNA stability, ribosome function, and the presence of inhibitors or activators.
Post-translational regulation involves the modification of proteins by factors such as phosphorylation, acetylation, and ubiquitination, which can affect protein stability, activity, and localization.
Overall, the flow of genetic information is a complex process that is regulated at multiple levels, ensuring the correct expression of genes in different cells and under different conditions.
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Explain how mycorrhizal fungi may have evolved from ancestors that were originally parasite of plant roots? Do N. Johnson's results indicate that present-day mycorrhizal fungi may act as parasites? Why?
Mycorrhizal fungi have possibly evolved from ancestors that were originally parasites of plant roots. N. Johnson's results suggest that present-day mycorrhizal fungi may act as parasites.
The present scenario, we will explain how mycorrhizal fungi may have evolved from ancestors that were originally a parasite of plant roots and why N. Johnson's results suggest that present-day mycorrhizal fungi may act as parasites. In the process of evolution, mycorrhizal fungi evolved from parasitic ancestors, colonizing the roots of plants. Mycorrhizal fungi form a mutualistic association with plants, which aids in the exchange of carbon for nutrients, resulting in the survival of both the plant and the fungus. The ancestor of mycorrhizal fungi was a parasitic fungus that colonized plant roots and extracted nutrients from them, as previously stated. The evolution of mycorrhizal fungi is believed to have started when the ancestor fungus was able to feed on root hairs without killing the host plants.
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- Alkaline phosphatase must NEVER be collected in which of the following tubes? A. Gray-topped tube B. Speckled-topped tube C. Red-topped tube D. Gold-topped tube
Alkaline phosphatase must NEVER be collected in speckled-topped tube. The correct option is C).
Alkaline phosphatase (ALP) is an enzyme commonly measured in clinical laboratory testing. Different types of tubes are used to collect blood samples for various tests. However, ALP must NEVER be collected in the speckled-topped tube.
The speckled-topped tube is typically used for immunology and serology testing, such as antinuclear antibody (ANA) testing. It contains a gel separator and clot activator.
The gel separates the serum from the clot, allowing for easy separation during centrifugation. However, this tube is not suitable for ALP testing.
ALP is measured in serum, and the presence of gel or clot activator in the speckled-topped tube may interfere with the accurate measurement of ALP levels. To ensure reliable ALP test results, it is important to use a tube specifically designed for serum collection. The correct option is C).
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1. Words that have evolved from a common ancestral word, showing
a closeness between related languages are called ?
A.Cognates
B.Phonemes
C.Morphemes
D.Kinesics
2. Humans are the only species known to
1. The option A is correct. A. Cognates.
2. Humans are the only species known to possess complex language and communication systems.
1. Words that have evolved from a common ancestral word, showing a closeness between related languages, are called cognates. Cognates are words in different languages that have a shared etymological origin, indicating a historical relationship between those languages.
Cognates often share similar or identical meanings and phonetic similarities, despite having undergone some phonetic and semantic changes over time. These shared characteristics are evidence of a common ancestral word that existed in the past.
For example, the English word "mother" and the Spanish word "madre" are cognates, both derived from the same ancestral word.
Cognates are particularly useful in the field of linguistics and language studies, as they provide valuable insights into language relationships, language families, and the historical development of languages.
By identifying and analyzing cognates, linguists can reconstruct proto-languages and trace language evolution.
2. Humans are unique among all known species in possessing complex language and communication systems.
While various animals communicate using sounds, gestures, or other forms of signals, human language stands out due to its complexity, creativity, and capacity for abstract and symbolic expression.
Human language is characterized by a vast vocabulary, grammar rules, syntax, and the ability to express a wide range of concepts and ideas. It allows for the transmission of knowledge, culture, emotions, and intentions in intricate and nuanced ways.
Additionally, humans have the ability to acquire multiple languages and use them for various purposes, such as communication, storytelling, and scientific discourse.
Although some animals exhibit rudimentary forms of communication, they typically lack the complexity and flexibility of human language.
While certain animals can learn to associate specific signals or symbols with simple meanings, they do not possess the same level of syntactical structure, creativity, and abstract reasoning that humans do.
Therefore, humans remain the only species known to possess the extraordinary linguistic abilities that define complex language and communication systems.
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Fill in the complementary DNA strand (template strand). Then transcribe \& translate these bacterial ORFs (open reading frame) from DNA sequence into mRNA / polypeptide. These are the non-template strands. 5'TCAATGGAACGCGCTACCCGGAGCTCTGGGCCCAAATTTCATTGACACT 3 ' 5′GGGATCGATGCCCCTTAAAGAGTTTACATATTGCTGGAGGCGTtAACCCCGGA 3 ′
Complementary DNA strand:3' AGTTACCTTGCGCGATGGGCCTCGAGACCCGGGTTAAAAGTAACGTGTG 5'Transcription is the process of producing an RNA molecule from a DNA template, while translation is the process of producing a polypeptide chain from an RNA molecule.
Transcription:5' UGAAUGGAACGCGCUACCCGGAGCUCUGGGCCCAAUUUCAUUGACACU 3'3' ACUUACCUUGCGCGAUGGGCCAGAGACCCGGGUUAAAAGUAAUGUGACUGAAUGUUAGGCGCGCUGACCCUGGUUGACU 5'mRNA:5' UGAAUGGAACGCGCUACCCGGAGCUCUGGGCCCAAUUUCAUUGACACU 3'3' ACUUACCUUGCGCGAUGGGCCAGAGACCCGGGUUAAAAGUAAUGUGACUGAAUGUUAGGCGCGCUGACCCUGGUUGACU 5'Polypeptide chain:5' Methionine-Asp-Asn-Cys-Ala-Cys-Lys-Thr-Pro 3'.
To find the complementary DNA strand (template strand), we can simply replace each nucleotide with its complementary base:
5' TCAATGGAACGCGCTACCCGGAGCTCTGGGCCCAAATTTCATTGACACT 3'
3' AGTTACCTTGCGCGATGGGCCTCGAGACCCGGGTTTAAAGTAACTGTGAA 5'
Now, let's transcribe each of the open reading frames (ORFs) into mRNA and translate them into polypeptides.
ORF 1 (Starting from the first AUG codon):
DNA: 5' TCAATGGAACGCGCTACCCGGAGCTCTGGGCCCAAATTTCATTGACACT 3'
mRNA: 3' AGUUAUCCUUGCUCGAUGGGCCUCGAGACCCGGGUUAAAUAAUGACACU 5'
Polypeptide: Ser-Tyr-Pro-Cys-Arg-Val-Ser-Asp-Pro-Gly-Phe-Lys-Ile-Cys-Th
ORF 2 (Starting from the second AUG codon):
DNA: 5' GGATCGATGCCCCTTAAAGAGTTTACATATTGCTGGAGGCGTtAACCCCGGA 3'
mRNA: 3' CCAUAGCUACGGGAUUUUCUCAAUUGUAUAACGACCUCCGCAttUUGGGGCCU 5'
Polypeptide: Pro-Tyr-Leu-Arg-Asp-Phe-Ser-Asn-Val-Asn-Asp-Pro-His-Leu-Gly-Pro
Please note that the lowercase "t" in the DNA sequence represents a potential mutation and should be interpreted as "T" when transcribing and translating.
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Which one of the following types of drugs has a narrow spectrum of activity and targets the etiologic agent for tuberculosis ?
A) Drugs which inhibit mycolic acid synthesis
B) Drugs which inhibit peptidoglycan synthisis
C) Drugs which inhibit protein synthesis
D) Drugs which inhibit folate synthesis
E) Drugs which inhibit nucleic aacid synthesis
Tuberculosis is a bacterial disease that affects the lungs and other parts of the body. Tuberculosis treatment is based on the severity of the disease.
The type of tuberculosis, and the medications that are available. Which one of the following types of drugs has a narrow spectrum of activity and targets the etiologic agent for tuberculosis?Drugs that inhibit mycolic acid synthesis have a narrow spectrum of activity and are aimed at the etiologic agent of tuberculosis.
This drug is one of the first-line antituberculosis drugs that have been approved for use in combination with other antituberculosis drugs for treating tuberculosis. Tuberculosis is caused by the bacterium Mycobacterium tuberculosis, and it can be treated with a combination of antibiotics.
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________ is seen when ABCDEFGHI becomes ABCFEDGHI.
A. Deletion
B. Duplication
C. Translocation
D. Transposition
E. Inversion
E.
The term that is seen when ABCDEFGHI becomes ABCFEDGHI is "inversion."What is an inversion?An inversion is a type of mutation that occurs when a chromosome segment breaks and then rearranges in the same location, but in the opposite direction.
It means that it changes the orientation of the genetic material in a segment of the chromosome. A chromosome segment can break into two places and reconnect in an inverted arrangement.Inversion can have several outcomes, including changing the order of genes, causing genes to fuse together, and even generating new genes. The significance of inversion varies depending on its location and the genes included in the inverted segment.
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