Peptides can be separated using an ion‑exchange column based on their isoelectric (pI) values. At which pH values would two different peptides, one with a pI of 5.3 and the other with a pI of 8.5 , bind to a cation‑ and anion‑exchange column? Each peptide may be capable of binding to each column at more than one pH value.

Answers

Answer 1

Answer:

At pH 3.5:

Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the anion-exchange column.

At pH 6.5:

Peptide A with pI 5.1 will bind to the cation-exchange column, while peptide B with pI 8.5 will bind to the anion-exchange column.

At pH 10.8:

Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the cation-exchange column.

Explanation:

Ion-exchange column chromatography is used for separating proteins and other molecules in a solution based on differences in net  charge. Negatively charged molecules bind to positively charged solid supports called the anion-exchange column, whereas, positively charged molecules bind to  negatively charged solid supports known as cation-exchange column.

A peptide can acquire a net charge if the pH of the medium is either above or below its isoelectric point,pI. At pH above pI, the peptide acquires a net negative charge and can bind to a positive support. At pH above pI, the peptide acquires a net positive charge and can bind to a negative support. The greater the difference between pH and pI, the tighter the peptide will bind to the column.

At pH 3.5:

Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the anion-exchange column.

At pH 6.5:

Peptide A with pI 5.1 will bind to the cation-exchange column, while peptide B with pI 8.5 will bind to the anion-exchange column.

At pH 10.8:

Peptide A with pI 5.1 and peptide B with pI 8.5 will both bind to the cation-exchange column.


Related Questions

What mass of solute should you measure out in order to make 1,000 mL of a 0.3 M solution of magnesium phosphate Mg3(PO4)2?

Answers

Hey there !

Molecular Weight: 262.86 g/mol

Volume in liters : 1,000 mL => 1,000 / 1000 => 1 L

Number of moles of solution :

number of moles = volume x molarity

number of moles = 1 x 0.3  => 0.3 moles

Therefore:

1 mole Mg₃(PO₄)₂ ------------------- 262.86 g

0.3 moles ----------------------------- mass of Mg₃(PO₄)₂  ??

mass of Mg₃(PO₄)₂ = 0.3 x 262.86 / 1

mass of Mg(PO₄)₂ => 78.858 g

Hope this helps!

______ bonds form when 2 metal atoms share electrons.

Answers

metallic i believe is the answer

The period that an element is in on the periodic table can tell you how many _____ the element’s atom have.

A. Energy level(electron shells)
B. valence electrons
C. protons
D. isotopes
WILL BE MARKED BRAINLIEST
PLUS 15 Points

Answers

Answer: It's D

Explanation:

A 12 gram piece of Cu at 475 oC is placed in contact with a 15 gram piece of Cr at 265 oC.

Assume the two pieces of metal are the system and that there is no heat exchange between system and surrounding.

What is the final temperature of the two pieces of metal expressed in degree C?

Answers

Answer:

349.22°C

Explanation:

Let the final temperature of the two pieces of metal be x.

Now, the warmer metal which is C u reduces from 475°C to x. Thus Δt for C u is; Δt1 = 475 - x.

The cooler metal Cr increases in temperature from 265°C to x. Thus, it's change in temperature is Δt for Cr is; Δt2 = x - 265.

Now from conservation of energy, the amount of energy leaving the C u metal is equal to the amount of energy entering the Cr metal.

Thus;

q_lost = q_gain

Where;

q_lost = m1•c1•Δt1

q_gained = m2•c2•Δt2

Now, c1 & c2 are the specific heat capacity of C u and Cr respectively.

From online tables, c1 = 0.385 J/g°C and c2 = 0.46 J/g°C

We are given;

m1 = 12g and m2 = 15g

Thus;

12 × 0.385 × (475 - x) = 15 × 0.46 × (x - 265)

2194.5 - 4.62x = 6.9x - 1828.5

6.9x + 4.62x = 2194.5 + 1828.5

11.52x = 4023

x = 4023/11.52

x = 349.22°C

A baseball has serval properties. Write one property that a baseball has.

complete sentences.​

Answers

The round cushioned cork pill at its core, the wool and poly/cotton windings in its midsection, and the cowhide covering that makes up its exterior.

hope this helps :)

What are some foods that bacteria helps in the production of?

Answers

Answer:

yeasts, moulds, bread, beer, wine, ...

Explanation:

The atom is one of the basic units of matter. Which part of the atom mostly determines the size of the atom?
a.Electrons

b.Nucleus

c.Neutrons

d.Protons

Answers

Answer:

Atomic size is the distance from the nucleus to the valence shell where the valence electrons are located. The separation that occurs because electrons have the same charge. The number of protons in the nucleus. The core electrons in an atom interfere with the attraction of the nucleus for the outermost electrons.

Explanation:

That would be B I believe, but please correct me if I'm wrong.

A resistor is placed in a circuit. The voltage of the circuit is changed and the current is measured. The graph seen here shows the data that was collected. Determine the resistance of the resistor in this circuit.

Answers

answer- 2 Ohms

explain- i took the test!

If the stack of bricks on the right has a mass of 240 kg, approximately what is the mass of the stack of bricks on the left?


A.
2400 kg
B.
720 kg
C.
320 kg
D.
480 kg

Answers

Answer:

720

Explanation: study island

Answer:

720

Explanation:

HELP
What type of bond is formed between the carbon and either oxygen in CO2?
A) Single
B) Double
C) Triple

Answers

It’s B Have Ah Good Day
It is B!! Good luck on you assignment

why saturated fatty acid can’t create kink ?​

Answers

Saturated fatty acids exhibit a linear structure while unsaturated fatty acids bend, or kink, due to double bonds within the chemical foundation.

A nuclear power plant is scheduled to undergo a retrofitting. What is the most likely outcome of the change?

The power plant will no longer generate radioactive wastes.
The power plant will recycle the water it uses to cool the reactor.
The power plant will be able to reprocess its used uranium fuel.
The power plant will switch from fission reactions to fusion reactions.

Answers

Answer:

It's B

Explanation:

I got it right on edjenuity

The most likely outcome of the retrofitting of a nuclear power plant is that it will be able to reprocess its used uranium fuel. Therefore, option C is correct.

What is nuclear power plant?

A nuclear power plant is a facility that generates electricity by harnessing the heat produced by nuclear reactions. At the heart of a nuclear power plant is the reactor, where a controlled chain reaction of nuclear fission occurs.

Reprocessing is a process by which the used nuclear fuel is chemically treated to separate out and recover the unused uranium and plutonium, which can then be reused as fuel. This reduces the amount of radioactive waste produced by the plant and also extends the life of the uranium fuel.

The fission reactions to fusion reactions is not currently a viable option for power generation, and the idea that a retrofitting would eliminate radioactive wastes is unlikely, as nuclear power plants will always produce some amount of radioactive waste.

Thus, option C is correct.

To learn more about the nuclear power plant, follow the link:

https://brainly.com/question/4246037

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The ethyl acetate solution containing the mixture used in this experiment had a concentration of 20.0 g/L of benzoic acid. 1. Recovery of benzoic acid:

Answers

Answer:

The correct answer is "[tex]\frac{0.65}{V} \ percent[/tex]".

Explanation:

The given values are:

[tex]M_r=0.130 \ g[/tex]

[tex]M_o=20 \ g/L\times V(L)[/tex]

     [tex]=20 \ V[/tex]

As we know,

⇒ [tex]Recovery \ percent=\frac{Amount \ of \ substance \ recovered}{Amount \ of \ substance \ originally \ taken}\times 100[/tex]

On substituting the given values, we get

                                [tex]=\frac{0.130}{20 \ V}\times 100[/tex]

                                [tex]=\frac{0.65}{V} \ percent[/tex]

Note: percent = %

What color do you get if you mix red and blue light?
Red and Green?
Blue and green?
What relationship do you find between frequency and energy?
What relationship do you find between wavelength and current flow?
What relationship do you find between light intensity and current flow?

Answers

Answer:

Purple light.

Yellow light.

Explanation:

Purple light and yellow light

C is the element symbol for which element?
a. Carbon
b. Cadmium
c. Celenium
d. Chlorine​

Answers

Answer:

A) Carbon

Explanation:

the others are wrong its A

Answer:

A) carbon

Explanation:

carbon or simply c has an atomic number of 6 and it has an atomic mass of 12.01 amu. it belongs to group 4a

Whenever the same two elements form more than one
compound, the different masses of one element that combine
with the same mass of the other element are in the ratio of
small whole numbers.
TRUE
FALSE

Answers

Answer:

The answer is true

Explanation: Because The law of multiple proportions states that when two elements react to form more than one compound, a fixed mass of one element will react with masses of the other element in a ratio of small, whole numbers.


I need the answer multiple choice

Answers

Answer:

it's answer is

element of an atom ✌️

Calculate the time required for a constant current of 0.868 A to deposit 0.387 g of Tl(III) as Tl(s) on a cathode.

Answers

Answer:

632.7 secs

Explanation:

Equation of the reaction:

Tl^3+ + 3e ------> Tl

If 1F = 96500 C

Q= It

Where;

I= current

t= time

3× 96500 C deposited 204 g of Tl

0.868 × t deposited 0.387 g of Tl

3 × 96500 × 0.387 = 204 × 0.868 × t

t= 3 × 96500 × 0.387/ 204 × 0.868

t= 632.7 secs

The time required for a constant current of 0.868 A to deposit 0.387 g of Tl(III) as Tl(s) is 2689 s

We'll begin by calculating the quantity of electricity needed to deposit 0.387 g of Ti

Ti³⁺ + 3e —> Ti

Recall:

1 mole of Ti = 48 g

1 electron (e) = 96500 C

Thus,

3 electrons = 3 × 96500 = 289500 C

From the balanced equation above,

48 g of Ti was deposited by 289500 C of electricity.

Therefore,

0.387 g of Ti will be deposited by = (0.387 × 289500) / 48 = 2334.09375 C of electricity

Finally, we shall determine the time required.

Quantity of electricity (Q) = 2334.09375 C

Current (I) = 0.868 A

Time (t) =?

t = Q / I

t = 2334.09375 / 0.868

t = 2689 s

Therefore, the time required for the reaction is 2689 s

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Which event is fast change that occurs as a result of plate tectonics?
A. Hurricane
B. Volcanic eruption
C. Formation of an ocean basin
D. Convection current

Answers

Answer: B. Volcanic eruption

Explanation:

A volcano is firmed when molten rocks is formed to the surface of the Earth. Volcanoes release molten rocks, ash, and gases to the Earth's surface.

Volcanoes are agents of change as the eruptions lead to the formation of new landforms and also destroy several things that they come in contact with.

Volcanic eruptions also occurs due to plate tectonics. Other events that happen due to plate tectonics also include earthquakes, and tsunamis.

Answer:B

Explanation:

Which of these solutions are basic at 25 °C ? A:[OH−]=2.53×10−7 M B:[H3O+]=9.99×10−9 M C:[H3O+]=6.31×10−4 M

Answers

Answer: frfg6

Explanation: 655tyyt

Which step is part of a scientific investigation?
O designing a solution
O implementing the solution
O evaluating the solution
O communicating the findings

Answers

Steps of a scientific investigation include identifying a research question or problem, forming a hypothesis, gathering evidence, analyzing evidence, deciding whether the evidence supports the hypothesis, drawing conclusions, and communicating the results.

Will give brainliest, Look at the graph above. Compare it to the periodic table. What do you notice about the location on the periodic table of the elements that represent the high points

Answers

Answer:

We can see that the atomic radius decreases across every row.

Explanation:

I will comment more below.

The elements that represent the highest points on the graph are all elements of group 1 and occur at the head of the period to which they belong.

The periodic table is an arrangement of elements in groups and periods. The elements in the same group have the same number of valence electrons while the elements in the same period have the same number of shells.

Looking at the graph, we can see that the high points in the graph all correspond to the group 1 elements; sodium, potassium, rubidium and cesium.The have the highest atomic radii in the period. Atomic radii increases down the group but decreases across the period. Remember that these elements are at the head of the period to which they belong hence they have the highest atomic radii.

Learn more: https://brainly.com/question/9352088

please help 10 points asap​

Answers

Answer:

opposite

Explanation:

Answer:

opposite

Explanation:

cu9c9ucoucucpucfpufupfpucupvpjvpucu

which of the following is a true for subtropical jet streams​

Answers

Answer: how do we answer when there are no options??

Explanation:

Please help
It’s super quick

Answers

Answer:

1. a

Explanation:

2. c

Answer:

1 is A number two is C

every spring has an equilibrium position which statement describe a spring at it equilibruim position?

Answers

The elastic potential energy is zero. The net force acting on the spring is zero. Explanation: The equilibrium position of a spring is the position that the spring has when its neither compressed nor stretched - it is also called natural length of the spring.Mar

17. Which is a fat-soluble vitamin?
O vitamin B
O vitamin C
O vitamin A

Answers

vitamin A is the fat soluble

Which of the following elements has 3 electrons in the 2p sublevel?
O A. O
O B. C
O C. B
O DN

Answers

Answer:

the answer is D. N

AP3X

According to electronic configuration, there are 3 electrons in the 2p sublevel ,hence the correct option is D.

Electronic configuration is defined as the distribution of electrons that are present in an atom or molecule in  an atomic or molecular orbitals.It describes how each electron moves independently in an orbital.

Knowledge of electronic configuration is important for understanding the structure of periodic table. It helps in understanding the chemical properties of elements.

Elements undergo chemical reactions so as to achieve stability. Main group elements obey the octet rule in their electronic configuration while the transition elements follow the 18 electron rule. Noble elements have valence shell complete in  their ground state and hence are said to be stable.

Thus, the correct option is D.

Learn more about electronic configuration,here:

https://brainly.com/question/29184975

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what mass of calcium chloride will be produced from 3.750 moles of hcl reacting with ca(oh)2

Answers

Answer:

208.125g

Explanation:

Given parameters:

 Number of moles of HCl = 3.75moles

Unknown:

 Mass of CaCl₂ that will be produced = ?

Solution:

The reaction equation is given as:

  2HCl  + Ca(OH)₂   →   CaCl₂ + 2H₂O

Now, to find the mass of CaCl₂, we have to work from the known to the unknown.

The known specie here is the number of moles of HCl;

        2 mole of HCl will produce 1 mole of  CaCl₂

       3.75moles of HCl will produce [tex]\frac{3.75}{2}[/tex]   = 1.875moles

The mass of  CaCl₂;

  Mass of  CaCl₂ = number of moles of  CaCl₂ x molar mass

Molar mass of  CaCl₂ = 40 + 2(35.5) = 111g/mol

  Mass of  CaCl₂ = 1.875 x 111 = 208.125g

Which one of the following is NOT a proper unit for frequency? *
m·s–1
Hz
s–1
1/s

Answers

Answer:

m*s⁻¹.

Explanation:

Hello!

In this case, since the frequency measures how repetitive an event is per unit of time, for instance the laps a car drives every 5 minutes or something similar, we can infer it has units of event/time; in such a way, the unit m*s⁻¹ is not a proper unit of frequency because it is a proper unit of velocity which is the distance traveled per unit of time.

In such a way, Hz (hertz), s⁻¹ and 1/s are possible ways frequency can be represented.

Best regards!

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