PCR amplification utilizes ________ template to produce a ________ product. For this question, select the answer that best describes the process as a whole: the nature of the template you used (the starting template added to the reaction) and the nature of the final product produced in your PCR analysis that you ran out on a gel will apply here.

Answers

Answer 1

Answer:

Double-stranded/Double-stranded

Explanation:

The PCR which is popularly known as polymerase chain reaction is the technique that uses the single copy of DNA to make thousands or levels a huge number(in terms of millions) of duplicates of that DNA.  

The PCR is a laboratory procedure that is utilized to make various duplicates of a section of DNA as depicted previously. It is an unmistakable strategy.  

There are 3 fundamental advances in PCR:

Denaturing  

Annealing  

Extending  

In denaturing measure requires warming of the double-stranded DNA template to isolate it into a solitary strand. Eventually, it resulted into a double-stranded item.


Related Questions

What is the molality of a 10.0 % by mass solution of NaCl?
(molar mass of NaCl = 58.44 g/mol)
mol/kg

Answers

On CK-12 all of these answers are correct

1.9

1.888

1.89

1.88

The molality of a 10.0 % by mass solution of sodium chloride (NaCl) is 1.7M.

How do we calculate molarity?

Molarity of any solution will be calculated as number of moles of solutes present in per litre of the solution.

Moles of the substance will be calculated as:

n = W/M, where

W = given mass

M = molar mass

Given that percent mass of NaCl = 10% i.e. 10g of NaCl is present in 100 g of solution.

We know that 1 g = 0.001 L

100 g = 0.1 L

Moles of NaCl = 10g / 58.44g/mol = 0.17 mol

Molarity of NaCl = 0.17 / 0.1 = 1.7M

Hence required molarity is 1.7 M.

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Of the three Intermolecular forces, the type with the largest dissociation energy is ________.
A. Dipole-dipole interactions
B. Dispersion forces
C. Hydrogen bonds​

Answers

Answer:

C. Hydrogen bonds​

Explanation:

Strongest intermolecular force

Hydrogen Bonding (H-Bonding)

Hydrogen bonds are caused by highly electronegative atoms. They only occur between hydrogen and oxygen, fluorine or nitrogen, and are the strongest intermolecular force.

Answer: C hydrogen bonds

Explanation:

Use the following balanced reaction to solve 1-3:
P4 (s) + 6H2 (g) → 4PH3 (g)

How many grams of phosphorus trihydride will be formed by reacting 60 L of Hydrogen gas with an excess of P4?

Answers

Answer:

60.86 g of PH₃

Explanation:

We'll begin by calculating the number of mole of H₂ that will occupy 60 L. This can be obtained as follow:

22.4 L = 1 mole of H₂

Therefore,

60 L = 60 / 22.4

60 L = 2.68 mole of H₂.

Next, we shall determine the number of mole of PH₃ produced by the reaction of 60 L (i.e 2.68 mole) of H₂. This can be obtained as follow:

P₄ + 6H₂ –> 4PH₃

From the balanced equation above,

6 moles of H₂ reacted to produce 4 moles of PH₃.

Therefore, 2.68 moles of H₂ will react to to produce = (2.68 × 4)/6 = 1.79 moles of PH₃.

Finally, we shall determine the mass of 1.79 moles of PH₃. This can be obtained as follow:

Mole of PH₃ = 1.79 moles

Molar mass of PH₃ = 31 + (3×1)

= 31 + 3 = 34 g/mol

Mass of PH₃ =?

Mass = mole × molar mass

Mass of PH₃ = 1.79 × 34

Mass of PH₃ = 60.86 g

Thus, 60.86 g of PH₃ were obtained from the reaction.

Express
as
ordinary numbers.
3 x 10^0=

Answers

any number to the power of zero is equal to 1
so 10^0=1 and 3 x 1 = 3 !

explain what you would do expect caesium astatide to look like .will it be soluble in water ?explain your reasoning​

Answers

Answer:

it will not be soluble in water Becoz it can only be

separated by passing it through silver nitrate solution

Explanation:

i hope you understand

How many fluorine atoms are there in 39.4 moles of CF4?

Answers

Answer:

here

Explanation:

Answer: 4

A molecule of carbon tetrafluoride, CF4 , is made up of 1 carbon atom bonded to 4 fluorine atoms. Each carbon atom weighs 12.0 amu and each fluorine atom weighs 19.0 amu so 1 CF4 molecule weighs 1×12.0 amu + 4×19.0 amu = 88.0 amu. This number, 88.0, is called the molecular weight (or molecular mass) of CF4.

Is this equation balanced: AgNO3 + 2 KCl -->AgCl + KNO3 Yes No

Answers

Answer:

No

Explanation:

AgNO₃ + KCl --> AgCl + KNO₃

This reaction is a precipitation reaction. AgCl is formed as a white solid due to its low solubility in water.

1,3,5,7-Cyclooctatetraene, C8H8, is an unusual hydrocarbon in that it reacts with exactly 2 equivalents of potassium to give A, C8H8K2, which can be isolated as a white solid. A exhibits a single proton NMR signal. Draw the structure of A

Answers

Answer:

Explanation:

The main assignment here is to provide the structure of A. But before that, let us explain briefly some certain things:

The proton NMR (1H) gives data about the quantity of non-identical protons present in the structure. The resonance frequency is seen regarding the standard compound (0 ppm), which is addressed as a chemical shift. The same protons have equal value of chemical shift.

The protons that are available in a similar environment are addressed as comparable protons and the protons with various conditions are called non-identical protons.

However, from the image below, we can see the reaction of the compound 1,3,5,7-cyclooctatetraene resulting in 2 equivalents of potassium forming an aromatic dianion.

- Explain why the term greenhouse effect is used to describe the theory of global
warming.
Does the greenhouse effect affect life on Earth? If yes, explain how?
What are the possible effects of a buildup of greenhouse gases in our atmosphere?

Answers

The greenhouse effect is a process that occurs when gases in Earth's atmosphere trap the Sun's heat. This process makes Earth much warmer than it would be without an atmosphere. It is one of the things that makes Earth a comfortable place to live.

A change resulting in one or more new substances being formed is a?

A. nuclear reaction
B.physical property
C. chemical change
D.physical change

Answers

Answer:

C. Chemical change

Explanation:

A physical change is where something is changed but it doesnt affect the build up of the chemical. For example, if you broke sticks and threw them on the ground, that would be a physical change because the change is happening to the physical being of the object and not its chemical buildup. However, if you lit those sticks on fire, that would be considered a chemical change because you end up with two substances, ash and the remnants of the stick. A nuclear reaction would result in something blowing up so its not that. And a physical property is like what it looks like or how it smells. Hope I helped you!

A compound accepts electrons from another substance to form a covalent bond. Which term best describes this
compound's behavior?
-Lewis acid
-Arrhenius base
-Bronsted-Lowry acid
-Bronsted-Lowry base

Answers

Answer:

A). Lewis Acid

Explanation:

deez

The term that best describes the compound that accepts electrons from another substance to form a covalent bond is Lewis acid.

What is Lewis acid?

Lewis acid is a compound or ionic species which can accept an electron pair from a donor compound while a Lewis base is a compound such as the OH- ion, that can donate a pair of non-bonding electrons.

According to this question, a compound accepts electrons from another substance to form a covalent bond.

Therefore, the term that best describes the compound that accepts electrons from another substance to form a covalent bond is Lewis acid.

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To prepare a buffer solution with a pH of 4.1, which acid should be used?
glycine: pka = 2.35
benzoic acid: pka = 4.19
hydrobromous: pKa = 8.7

Answers

Answer:

benzoic acid: pka = 4.19

Explanation:

How many moles is equal 82.4 grams of CO,?

Answers

Answer:

1 mole = 28.0101

Hence,

82.4 = around 2.8 mole

Have a great day <3


Which general equation represents an endothermic reaction?
A. reactants - energy - products + energy
B. reactants
products + energy
C. reactants + products - energy
D. reactants + energy
products

Answers

Explanation:

The general equation for an endothermic reaction is: Reactants + Energy → Products.

Hope it helps :)❤

The equation represents an endothermic reaction is reactants + energy

products.

What is an endothermic reaction?

Endothermic reactions are chemical reactions in which the reactants soak up warm strength from the surroundings to form products.

net energy is not released.heat is not evolved with the product.heat is required to start the reaction.increase with the increase in temperature.decrease with a decrease in temperature.

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The fizz in the soda that we drink is carbonic acid. Leaving the soda can open to the environment separates the carbonic acid into water and carbon dioxide. What type of reaction is this and what is the resulting product?

Answers

Answer:

The reactionnis known as a decomposition reaction

The equation of the reaction is given below as:

H₂CO₃ -----> H₂O + CO₂

Explanation:

A reaction in which a single compound is split into two or more simpler substances is known as a decomposition reaction.

In the reaction in which when a soda can is opened and left to the environment, the fizz in the soda, carbonic acid, separates into water and carbon dioxide is an example of a decomposition reaction.

The equation of the reaction is given below:

H₂CO₃ -----> H₂O + CO₂

In the reaction above, a larger molecular weight compound, carbonic acid with the molecular, H₂CO₃ is splits into two simpler substances of smaller molecular weight, water, H₂O and carbon dioxide, CO₂.

This reaction rate is made faster by an increase in temperature and by a decrease in pressure of the soda.

what career field/job would utilize ppm units
A environmental jobs
B Medical fields
C Jobs at the brewery
D Jobs in analytical chemistry

Answers

Jobs in analytical chem tht is because it’s parts per million and when calculating the amount of an element in a mole

MATch THE BOXED PLEASEEEE

Answers

Answer:

Transform = The third one

Convergent  = the first one

Divergent = the second one

Explanation:

divergent: divide (separate)
convergent: collide
transform: slide past

In Which State of matter are molecules moving the slowest
A. Solid
B. Liquid
C. Gas

Answers

Answer:

Solid

Explanation:

Molecules in solids only vibrate in place, while the other two have moving molecules

It is solid because it has the most tightly packed Particles

How many grams of ( S ) - 1 - chloro - 4 - ethyl - 2 - methylhexane and triphenylphosphine would you need to create 4 . 1 5g of ( S ) - 1 - chloro - 4 - ethyl - 2 - methyl triphenylphosphonium assuming an 8 1 % yield for the reaction?

Answers

Answer:

Explanation:

The standard molar mass is:

For (S )-1-chloro-4-ethyl-2-methylhexane = 162.5 g/mol

For triphenylphosphine  = 262 g/mol

For  ( S )-1-chloro-4-ethyl-2-methyl triphenylphosphonium = 424.5 g/mol

The mass required for 81% yield = [tex]\dfrac{81}{100} \times Theoretical \ yield = 4.15 g[/tex]

Theoretical yield = [tex]\dfrac{4.15}{0.81}[/tex]

= 5.1235 g

thus, since 424.5 g yield produce from 162.5 g

5.1235 g yield will produce = [tex]\dfrac{162.5}{424.5}\times 5.1235 \ g[/tex]

= 1.9613 g of alkyl halide (-chloro) required.

Also, since 424.5 g yield produce from 262 g phosphine

5.1235 g yield will produce = [tex]\dfrac{262}{424.5}\times 5.1235 \ g[/tex]

= 3.1622 g of triphenylphosphine required.

You have 281 kilojoules how many kilocalories is it need to know ASAP

Answers

67.116 kilocalories is the answer

I don’t understand please help

Answers

It’s C)H2SO4
Good luck
I agree the answer is C according to my research about it

What is a similarity between a fish embryo and a human embryo in the late stages of development?
They both have tails
They both have gills.
They both have spines.
They both have arms.

Answers

They both have gills

Answer:In the LATER stages of development they would have a spine

Explanation:

What is the concentration of a 500 mL solution with 25 mol of HF? Write your answer with TWO decimal places and round accordingly.

Answers

Answer:

oop I'm thinking hmmmmm

What is the function of white blood cells?
A) immunity
b) carry nutrients, waste
C) blood clotting

Answers

Answer:

A

Explanation:

They help fight infection and other disease

Answer:

immunity is the answer for this question

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