In the hydrolysis of p-nitrophenylacetate by chymotrypsin all of the following are correct (mark all the correct answers): Group of answer choices the active site contains a serine residue, an unprotonated histidine, and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate. attack of a water molecule on the acyl-enzyme intermediate yields the second product. the first product is acetic acid. an acetyl-enzyme intermediate forms during the mechanism.

Answers

Answer 1

Answer:

-- the active site contains a serine residue, an unprotonated histidine, and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate.

--  attack of a water molecule on the acyl-enzyme intermediate yields the second product.

-- an acetyl-enzyme intermediate forms during the mechanism.

Explanation:

p-Nitrophenyl acetate is a chemical compound and is used as a substrate in the assays for esterase as well as lipase activity.

Doing hydrolysis of the p-nitrophenyl acetate releases a p- nitrophenol, and its absorbance can be monitored at about 405 nm.

In doing hydrolysis of the p-nitrophenyl acetate by a chymotrypsin, the following holds true :

By hydrolysis, the acetyl enzyme intermediate is formed.an attack of the water molecule on acyl enzyme yields a second productthe site consists of a serine residue, unprotonated aspartate and an unprotonated histidine residue at pH

Related Questions

The specific heat capacity of water is 4.186 J/g °C
Calculate the temperature change when 8000 J of heat is added to 3g of water.
Use the equation q=mcT

Answers

Answer:

Solution given:

specific heat capacity of water [c]4.186 J/g °C

temperature[∆T]=?

mass[m]=3g

heat[Q]=8000J

we have

Q=mc∆T

8000=3*4.186*∆T

∆T=8000/12.558

∆T=637.04°C

the temperature change is 637.04°C.

Write a neutralization reaction to form the salt k2so4

Answers

H2SO4 + 2KCL ----------› K2SO4 + 2HCL

hope it helps you ♡♡

Complete the missing information in the reactions. Then, label each reaction as Alpha
(a) or Beta (B) decay.
14
0
N
9
Type: :
n:
p:
n:
p:
n:
P:
238
U
10. 92
234
Th
90
+
Type: :
n:
n:
p:
n:
p:
I
p:
0
32
11 15
P
Type: :
n:
n:
n:
p:
P:
P:
4
244
Pu
12 94
He
Type:
n:
n:
n:
p
p:
p:

Answers

Answer:

rtyuioroiytryu

Explanation:

Lithium chloride + Iron → ?

Answers

Answer:

Lithium chloride + Iron

No reaction takes place, because lithium is more reactive than iron, so Iron cannot displace lithium from its solution.

Hope u understand

Please mark as brainliest

Thank You

What volume of O2 at STP is required to oxidize 8.0 L of NO at STP to NO2? What volume of NO2 is produced at STP?

Answers

The balanced equation of the reaction is:

[tex]O_2 + 2 NO[/tex] ⇒ [tex]2 NO_2[/tex]

Every gas occupied 24 L in one mole in r.t.p. according to the equation:

[tex]24 \times n = V[/tex]

8/24=0.333 mol

2 moles of NO makes 2 moles of [tex]NO_2\\[/tex] so

0.333*24=8L

ano ang kahulugan ng sekswalidad?

Answers

ano ang naakit sa isang tao..at kung ano ang iyong kasarian ay

When you perform a stoichiometry calculation, you are determining the ...
A) actual yield
B) theoretical yield
C) percent yield

Answers

The actual yield is expressed as a % of the theoretical yield . (Percent yield )

Answer:

B) Theoretical

Explanation:

Just did the question

Which is the correctly balanced chemical equation for the reaction of KOH and H2SO4? Which is the net ionic equation for the reaction?

Answers

Answer:

See explanation

Explanation:

A balanced chemical reaction equation has the same number of atoms of each element on both sides of the reaction equation.

Hence, for the reaction between KOH and H2SO4, the balanced chemical reaction equation is;

H2SO4(aq) + 2KOH(aq) ---------> K2SO4(aq) + 2H2O(l)

Complete ionic equation;

2H^+(aq) + SO4^2-(aq) + 2K^+(aq) +2OH^-(aq) -------> SO4^2-(aq) + 2K^+(aq) + 2H2O(l)

Net ionic equation;

2H^+(aq) + 2OH^-(aq) -------> 2H2O(l)

what is used to observe things, like cells, that are too small to see with the naked eye.

Answers

A microscope is an instrument that can be used to observe small objects, even cells. This lens bends light toward the eye and makes an object appear larger than it actually is. ...

Answer:

a microscope is used to see little things the naked eye can't

3. How many atoms in 3.91 moles of sodium?

Answers

Answer:

Explanation:

The formula for sodium is Na. It does not form a molecule in some way.

1 mol Na = 6.02*10^23 atoms

3.91 mol = x                                    Cross multiply

x = 3.91 * 6.02 * 10^23

x = 23.65 * 10^23

x = 2.365 * 10^24

Scientific notation is always expressed as a number 1 ≤ x < 10

when electrical energy is converted into any other deform of energy, the total amount of energy

Answers

the answer is mechanical

what makes a compound a pure substance ​

Answers

Answer:

Compounds contain more than one type of material. Yet both compounds and elements are considered pure substances. 

Pure compounds are created when elements combine permanently, forming one substance

Extra:

So, a mixture can be separated into its original components, while a pure compound cannot.

When naming compounds part of the second element's name is dropped and what is added in its place?
ude
ode
ede
ide

Answers

Answer:

ide is the correct answer

Explanation:

example compound of sodium and hydrogen in sodium hydroxide

and compound of hydrogen and chlorine is hydrogen chloride

QUICK PLEASE
What is the final concentration when 275 mL of 20.0 % W/V hydrogen peroxide solution is diluted to 1.000 L?

20.0 % W/V
0.30 % W/V
55.0 % W/V
5.50 % W/V

Answers

Answer:

20.0% w/v A

Explanation:

A 20.0 w/V...

HELPPP!!!
The emission of electromagnetic energy from an atomic nucleus is called_______
decay.

Answers

Gamma decay, type of radioactivity in which some unstable atomic nuclei dissipate excess energy by a spontaneous electromagnetic process. In the most common form of gamma decay, known as gamma emission, gamma rays (photons, or packets of electromagnetic energy, of extremely short wavelength) are radiated.

Which of the following measurements contains 2 significant figures?
A 0.0240
С 0.0901
B 371
D 1.0x10
E 0.004

Answers

The answer would be 371 because it has multiple complete digits

At the carnival, a baby was given a helium balloon whose
pressure equaled the atmospheric pressure at the carnival
which was 102.7 kPa. However, the baby wasn't paying
attention and the balloon floated away into the troposphere
where the atmospheric pressure is 97.8 kPa. What is the
positive difference between the pressure in the balloon and
the pressure of the troposphere in atm?
atm

Answers

Answer: 0.048 kPa

Explanation: no clue i just looked it up you’re welcome y’all:)

Which of the following solutions is the weakest base?
a) pH 2.3
b) pH 4.6
c) pH 6.6
d) pH 7.9
e) 12.5

Answers

Answer:

solution d with pH of 7.9

How many molecules are in 0.0230 grams of C3H6?

Answers

I think so the answer is 0.0005465813239340357

How is an alkoxide ion formed??

Answers

An alkoxide is an organic functional group formed when a hydrogen atom is removed from a hydroxyl group of alcohol when reacted with a metal. It is the conjugate base of alcoho.
The answer above is completely correct :)

Can you please help me with this?

What happens with the evaporation of raindrops in the atmosphere?

Answers

Answer:

they become water vapor and after that, they become a type of precipitation

The evaporation of a drop of water in an air stream is determined by the rate of transfer of heat and water vapour between the drop surface and the ambient air. If these transfer processes are governed by molecular diffusion the appropriate equations take a well known form. I hope this can help

HNO3 + S --> H2SO4 + NO Now identify the element oxidized and the element reduced. Which element is oxidized? Which element is reduced? Which elements undergo no oxidation or reduction?

Answers

Answer: S is getting oxidized, N is getting reduced and O and H undergo no oxidation or reduction

Explanation:

The oxidation reaction is defined as the reaction in which a chemical species loses electrons in a chemical reaction. It occurs when oxidation number of a species increases.

A reduction reaction is defined as the reaction in which a chemical species gains electrons in a chemical reaction. It occurs when oxidation number of a species decreases.

For the given chemical reaction:

[tex]HNO_3+S\rightarrow H_2SO_4+NO[/tex]

On the reactant side:

Oxidation number of H = +1

Oxidation number of N = +5

Oxidation number of O = -2

Oxidation number of S = 0

On the product side:

Oxidation number of H = +1

Oxidation number of N = +2

Oxidation number of O = -2

Oxidation number of S = +6

As the oxidation number of S is increasing from 0 to +6. Thus, it is getting oxidized. Similarly, the oxidation number of N is decreasing from +5 to +2. Thus, it is getting reduced.

The oxidation numbers of O and H remain the same on both sides of the reaction. Thus, they are neither getting oxidized or reduced.

Hence, S is getting oxidized, N is getting reduced and O and H undergo no oxidation or reduction

Se tiene una solución acuosa 2M de carbonato de potasio. Expresar su concentración en %p/v y Normalidad.

Answers

Answer:

Normalidad = 4N

%p/V = 27.6%

Explanation:

La solución 2M de carbonato de potasio contiene 2moles de carbonato por litro de solución. La normalidad son los equivalente de carbonato de potasio (2eq/mol) por litro de solución:

2moles * (2eq/mol) = 4eq / 1L = 4N

El porcentaje peso volumen es el peso de carbonato en gramos dividido en el volumen en mL por 100:

%p/V:

Masa K2CO3 -Masa molar: 138.205g/mol-

2moles * (138.205g/mol) = 276g K2CO3

Volumen:

1L * (1000mL/1L) = 1000mL

%p/V:

276g K2CO3 / 1000mL * 100

%p/V = 27.6%

If you sprain your ankle and place a cold pack on it, how does the motion of molecules in your ankle change?​

Answers

Answer:

that heat energy speeds up the motion of the particles and the temperature of the reaction

Explanation:

HOPE IT HELPS

When we place an ice pack on a sprained ankle, the blood flow is reduced which prevents  further swelling, ice reduces the blood flow which is necessary  when  the ankle is injured.

What happens  after placing an ice pack?

The flow of blood and the lymph vessel is limited or decreased after applying an ice pack, this is an immediate  first aid to be given to the injured person

After 2-3 days of the injury, a hot  pack should  be applied to increase the blood flow and the lymph flow for an activity.

Hence, the molecule flow is reduced after applying an ice pack to the sprained ankle

learn more about molecules

https://brainly.com/question/19922822

#SPJ2

Help pleaseeee

you wish to make a potassium carbonate solution with a molar concentration of 0.25mol/dm³,but you only have 11.7g of potassium carbonate. what is the maximum volume of solution you can make?​

Answers

Answer:

0.337 dm³

Explanation:

M(K2CO3) = 138.2 g/mol

1) 11.7 g * (1 mol/138.2 g) =0.08466 mol

2) 0.08466 mol * (1 dm³/0.25 mol) = 0.3386 dm³ ≈ 0.337 dm³

Si de una caja de gelatina de 30g, se obtiene 5 porciones. Cuantas cajas y cuantos gramos se requieren para preparar 100 porciones.? respuestas concretas por fis

Answers

Answer:

20 cajas - 600 gramos

Explanation:

Veamos los datos informados:

1 caja → 5 porciones

1 caja → 30 g

Si queremos preparar 100 porciones, indudablemente necesitamos:

100 porciones . 1 caja / 5porciones = 20 cajas

Finalmente ya sabemos que una caja tiene 30 g

Entonces, si tenemos 20 cajas . 30 g / 1 caja = 600 g

En conclusión:

Necesitamos una masa total de 600 gramos  en 20 cajas

If you took 800mg of aspirin tablet at 12:00pm and another 800mg tablet at 2pm, how many milligrams of aspirin are still in your system at 3pm? Aspirin has a t1/2 of 1 hour.

Don't you dare put a link. I have a very specific set of skills >.>
(Chemistry is unfortunately not one of them though, so please help me ^-^)

Answers

Answer:

100mg are in your system at 3pm

Explanation:

Half-life is the time necessary to decrease the initial amount of a substance in the half.

At 12:00pm, the amount of aspirin is 800mg

As half-life is 1h,

at 1:00pm, the amount of aspirin is 800mg/2 = 400mg

At 2:00pm, 400mg/2 = 200mg

At 3:00pm, 200mg/2 =

100mg are in your system at 3pm

What are the units for the Volume of any Polyhedron?

Answers

Explanation:

A prism is a polyhedron with two parallel, congruent faces called bases that are polygons . The volume of a 3 -dimensional solid is the amount of space it occupies. Volume is measured in cubic units ( in3,ft3,cm3,m3 , et cetera).

Answer:

A cube with side length 1 unit, called a “unit cube,” is said to have “one cubic unit” of volume, and can be used to measure volume.

Which statement about Niels Bohr’s atomic model is true?

Answers

Answer:

Each orbit has a specific energy level.

Would you mind marking it the brainliest:).

How many moles are in 3.90 x1030 atoms of Zn?

Answers

Explanation:
The problem basically wants you to find a way to convert between the number of atoms present in the sample and the number of moles they are equivalent to.
To convert between atoms and moles we use something called Avogadro's constant, which basically acts as the definition of a mole.
More specifically, in order to have one mole of an element you need
6.022 x 10^23 atoms of that element. You can thus use this number as a conversion factor to take you from atoms to moles or vice versa.
In your case, you will have


3.90 x 10^ 26 atoms Zn x 1 mole Zn ( Avogrado’s constant) / 6.022 x 10^23 atoms Zn

= 6.5 x 10^8 is the answer
Other Questions
Mosquitoes are classic examples ofA.commensalismB.endoparasiteC.ectoparasiteD.mutualism an inclined plane rises to a height of 2m over a distance of 6m calculate the angle of the slope Calculate the boiling point of 3.42 m solution of ethylene glycol, C2H602, in water (Kb =0.51C/m, the normal boiling point of water is 100C): If A and B are mutually exclusive events, then P(A or B) = P(A) + P(B).andIf A and B are inclusive events, then P(A or B) = P(A) + P(B) - P(AB).Si hay 30% de probabilidad de que neve y llueva en da de hoy. Si la probabilidad delluvia es 40% y la probabilidad de nieve es de 15%. Cul es la probabilidad de que cadanieve o llueva en el da de hoy? A spinner has 2 blue spaces, 5 yellow spaces, and 1 orange space. If the spinner is spun once, what is the theoretical probability of the spinner NOT stopping on orange? P(Not orange) = The answer choices are 1. Square pyramid 2. Cube 3. Triangular prism Gretchen made a paper cone to hold a gift for a friend. The paper cone was 15 inches high and had aradius of 3 inches. Find the volume of the paper cone to the nearest tenth. Use 3.14 for it.The volume of the paper cone is about. in. Based on the information in the diagram, what is the area of AMNP ?1251000750105 Solve the system of equations by graphing. 6x 2y = 6 x + 2y = 8 The vast majority of research literature has taught us that reward is much more effective than punishment in caring for our children. This is also true with our beloved doggies, yet out of frustration and lack of control, some owners hit their dogs as a method of training. The most common example is when a dog is being trained to be house broken (only urinating and defecating outdoors). It would stand to reason that moments of accidents (which are not only to be expected but) are opportunities for learning and training. Unfortunately, this is not always the case. I have witnessed owners yelling at and spanking their dog when they have discovered poop in their homes and the dog of course responds by cowering. If this persists (just more than once is all it takes), what happens then is that every time an owner raises their hand quickly (regardless of the reason; swatting a mosquito, gesticulation), the dog flinches with fear. How much power is required to light a lightbulb at 100V of voltage when the lightbulb has a resistance of 500 Ohms? Por qu el Dumping es considerado una prctica desleal como estrategia comercial? Por qu afecta a los pases perifricos? If d= the number of dogs, which variable expression represents he phase below? the sum of number of dogs and the 6 cats Please help out! I cant solve this The Fun Tyme Toy Company discovers that one of its products can easily break, exposing children to potential injury from the sharp parts. Under the Consumer Product Safety Act of 1972, how long does Fun Tyme have to report this defect to the Consumer Product Safety Commission (01.02 LC)Which of the following describes a function of a satellite? Helps scientists track weather Measures latitude and longitude Calculates the altitude of landforms Determines population in a city PLS HURRY, DUE TODAYNow that you have a better understanding of the differences between the male and female hero story, lets get creative! Your task is to develop an outline for a contemporary female hero story. You must:1. Create a female hero2. Develop a goal for your hero to achieve3. Detail the stages of your heros journey4. Reflect on the significance of your heros story in our current society (Why is the story important?)Use the following organizer to help you create your outline. The managing underwriter has set the POP for a new issue offering at $50 per share. After negotiating, the proceeds to be received by the issuer upon closing are set at $48 per share. The managing underwriter will retain a management fee of $.25 per share and the selling concession has been set at $.75 per share. The spread is: A $2.00 B $1.25 C $1.00 D $.25 if 3 drinks cost $9 how much will 5 drinks cost evaluate (3.02)(0.5)=