If the concentration of a sample is 1.704 ug/ul and it needs to be diluted to .75 ug/ul with a final sample volume of 25 ul, how much buffer would be needed for the proper dilution?

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Answer 1

To dilute the sample to a final concentration of 0.75 ug/ul with a total volume of 25 ul, you would need approximately 11.06 ul of buffer.

To calculate the amount of buffer needed for the proper dilution, we can use the formula:

C1V1 = C2V2

Where:

C1 = Initial concentration

V1 = Initial volume

C2 = Final concentration

V2 = Final volume

Given:

C1 = 1.704 ug/ul

V1 = Volume of the initial sample (unknown)

C2 = 0.75 ug/ul

V2 = 25 ul

We need to solve for V1, the initial volume of the sample.

Using the formula, we can rearrange it as:

V1 = (C2 * V2) / C1

Substituting the given values:

V1 = (0.75 ug/ul * 25 ul) / 1.704 ug/ul

V1 ≈ 11.06 ul

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