g (2pts) A 10x transfer buffer solution is 250mM Tris and 1.92M glycine. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so we will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and glycine in the 1x buffer

Answers

Answer 1

Answer:

The explanation according to the given question is summarized below.

Explanation:

Given:

Tris,

= 250 mM

Glycine,

= 1.92 M

According to the solution,

For the dilution pf 10X to 1X buffer, we get

= [tex]1 \ ml \ of \ 10X \ buffer +9 \ ml \ of \ distilled \ water[/tex]

= [tex]10[/tex]

i.e.,

⇒ [tex]10X \ to \ 1X=1:10 \ dilution[/tex]

Now,

⇒ [tex]10X (250 \ mM\ Tris \ HCl, 1.92M\ Glycine, and\ 1 \ percent (\frac{w}{v} ) SDS) ---->1X(25 \ mM \ Tris \ HCl,0.193 M\ Glycine, and \ 0.1 \ percent(\frac{w}{v} )SDS)[/tex]


Related Questions

What would the IUPAC name be?

Answers

Answer:

methyl ethanoate

Explanation:

To name the compound given above, the following must be obtained:

1. Determine the functional group of the compound.

In this case, the functional group is R–COOR' where R and R' are alkyl groups. Thus, the compound is an ester.

2. Determine the longest chain before the functional group and the compound after the functional group.

In this case, the longest chain before the functional group is carbon 2 i.e ethane and the compound after the functional group is methyl.

3. Name the compound by naming the compound after the functional group first, followed by the compound before the functional group and ending it with –oate.

This is illustrated below:

After the functional group => methyl

Before the functional group => ethane

Name of the compound => methyl ethanoate

Silver nitrate, AgNO3, reacts with ferric chloride, FeCl3, to give silver chloride, AgCl, and ferric nitrate, Fe(NO3)3. In a particular experiment, it was planned to mix a solution containing 25.0 g of AgNO3 with another solution containing 45.0 grams of FeCl3. Write the chemical equation for the reaction.​

Answers

Answer:

[tex]\mathbf{FeCl_3 + AgNO_3 \to 3 AgCl+Fe(NO_3)_3}[/tex]

Explanation:

From the given question,

The chemical equation is:

[tex]\mathbf{FeCl_3 + AgNO_3 \to 3 AgCl+Fe(NO_3)_3}[/tex]

We are given that:

mass of [tex]FeCl_3[/tex] = 45 g

number of moles of [tex]FeCl_3[/tex]  = 45 g/162.2 g/mol

= 0.28 mol

mass of [tex]AgNO_3[/tex] = 25g

number of moles of [tex]AgNO_3[/tex]  = 25/169.87

= 0.147 mol

From the given equation 1 mole of [tex]FeCl_3[/tex] is required to make 3 mole of [tex]AgNO_3[/tex]  

0.28 mole of [tex]FeCl_3[/tex] = 0.28 × 3 = 0.84 mol of [tex]AgNO_3[/tex]  

Here [tex]AgNO_3[/tex]  is the limiting reagent.

Thus,

3 mole of [tex]AgNO_3[/tex]  = 3 moles of AgCl

0.147 mole of [tex]AgNO_3[/tex] = 0.147 mole of AgCl produced.

(1 point) Which compound produces the greatest number of ions when one mole of it is dissolved in water

Answers

The question is incomplete, the complete question is;

Which produces the greatest number of ions when one mole dissolves in water? a. NaCl b. NH4Cl c. NH4NO3 d. Na2SO4

Answer:

Na2SO4

Explanation:

If we consider the compounds listed in the options one after the other;

NaCl produces two moles of ions in solution

NH4Cl produces two moles of ions in solution

NH4NO3  produces two moles of ions in solution

Na2SO4 produces three moles of ions in solution

We can see that Na2SO4 produces the greatest number of ions when one mole of the substance  is dissolved in water, hence the answer above.

Given 32.0 g of water, if we see a temperature change from 25.0°C to 20.0°C, then how much heat energy (q) is transferred from the water?

(The specific heat of water is 4.184 J/g°C )

Answers

Answer:

Q = 669.44 J

Explanation:

Given that,

Mass of water, m = 32 g

The temperature change from 25.0°C to 20.0°C.

We need to find the amount of heat energy transferred. Let it is Q. We know that,

[tex]Q=mc\Delta T[/tex]

Where

c is the specific heat of water

Put all the values,

[tex]Q=32\times 4.184 \times (20-25)\\Q=669.44\ J[/tex]

So, 669.44 J of heat energy is transferred from the water.

How do isotopes of the same atom react chemically? How do isotopes of the same atom compare in size?

Answers

Atoms of the same elements differing in the number of neutrons in their nuclei are known as isotopes. Thus, isotopes of an element have the same atomic number but different atomic mass number. Isotopes of an element have similar chemical properties but different physical properties.

What is the total mass of products formed when 64.18 grams of CH4 is burned with excess oxygen?

Answers

80 grams of product is formed when 16 grams of CH₄ is burnt in excess of Oxygen so ur answer is

80 GRAMS

Can someone help me with a bio organic questions??‍♀️

Answers

Yeah what is the question
I believe the answer could be “alkanes”

How many moles of O2 did you produce the 8.31 moles of H2O

Answers

the equation is not present.

7. In science, the process/thing that you examine/focus on is called the ??
and anything outside of this focus is called the ??

Answers

Answer:

Subject of the experiment and out of scope

Explanation:

In any research study, the process of thing studied is basically the subject of the experiment on which research is conducted to determine the outcomes. If any thing lies out of the experimental process reach, then it is said to be out of scope of the experiment.

When you test starch with Barfoed’s reagent, what would be the answer, positive or negative?

Answers

Answer:

It would test positive.

Given the reaction: N2 + O2 = 2NO for which the Keq at 2273 K is 1.2 x 10-4
a. Write the equilibrium constant expression for the reaction.
b. Write the equation that would allow you solve for the concentration of NO.
c. What is the concentration of NO if [NZ] = 0.166M and [02] = 0.145M?

Answers

Answer:

(a): The expression of equilibrium constant is [tex]K_{eq}=\frac{[NO]^2}{[N_2][O_2]}[/tex]

(b): The equation to solve the concentration of NO is [tex][NO]=\sqrt{K_{eq}\times [N_2]\times [O_2]}[/tex]

(c): The concentration of NO is 0.0017 M.

Explanation:

The equilibrium constant is defined as the ratio of the concentration of products to the concentration of reactants raised to the power of the stoichiometric coefficient of each. It is represented by the term [tex]K_{eq}[/tex]

(a):

The given chemical equation follows:

[tex]N_2+O_2\rightarrow 2NO[/tex]

The expression for equilbrium constant will be:

[tex]K_{eq}=\frac{[NO]^2}{[N_2][O_2]}[/tex]

(b):

The equation to solve the concentration of NO follows:

[tex][NO]=\sqrt{K_{eq}\times [N_2]\times [O_2]}[/tex]            ......(1)

(c):

Given values:

[tex]K_{eq}=1.2\times 10^{-4}[/tex]

[tex][N_2]_{eq}=0.166M[/tex]

[tex][O_2]_{eq}=0.145M[/tex]

Plugging values in equation 1, we get:

[tex][NO]=\sqrt{(1.2\times 10^{-4})\times 0.166\times 0.145}[/tex]

[tex][NO]=\sqrt{2.88\times 10^{-6}}[/tex]

[tex][NO]=0.0017 M[/tex]

Hence, the concentration of NO is 0.0017 M.

Pick the correct statement about the pure water. Group of answer choices Pure water contains no ions. Pure water contains equal amounts of hydroxide [OH-] and hydronium [H3O ] ions. Pure water contains larger amounts of hydroxide [OH-] than hydronium [H3O ] ions. Pure water is an electrolyte. Pure water contains smaller amounts of hydroxide [OH-] than hydronium [H3O ] ions.

Answers

Answer:

Pure water contains no ions.  TRUE

Pure water contains equal amounts of hydroxide [OH-] and hydronium [H3O+ ] ions.  TRUE

Explanation:

This is the equilibrium for pure water:

2H₂O  ⇄  H₃O⁺  +  OH⁻          Kw

We see that pure water has no Ions. Pure water can not conduct electricity.

Generally ionized water comes from the water tap.

Another feature of pure water is pH.

Definetely pH of pure water is : 7

As pH = 7, [H₃O⁺] = 1×10⁻⁷

Then, [OH⁻] = 1×10⁻⁷

This is reazonable because Kw is 1×10⁻¹⁴ and Kw = [H₃O⁺] . [OH⁻]

In conclussion:

Pure water contains no ions.  TRUE

Pure water contains equal amounts of hydroxide [OH-] and hydronium [H3O+ ] ions.  TRUE

Pure water contains larger amounts of hydroxide [OH-] than hydronium [H3O+] ions.  FALSE

Pure water is an electrolyte. FALSE

Pure water contains smaller amounts of hydroxide [OH-] than hydronium [H3O ] ions. FALSE

explain the trend in boling point of alcohols in their homologous series​

Answers

Explanation:

The boiling point of alcohols also increase as the length of hydrocarbon chain increases

The boiling point of alcohols also increase as the length of hydrocarbon chain increases.

Why boiling points of alcohols increases as the length of hydrocarbon chain increasesSince, boiling point depends upon type of bonds present in the molecules.If molecules have hydrogen bonding then boiling point is higher than others because hydrogen bond is stronger bond.So, higher energy or temperature required to break these bond or overcomes the inter molecular force of attraction.Since, alcohol contains hydrogen bonding. So, its boiling points are higher as compare to other compounds.Since, boiling points is also directly proportional to molar mass of molecules. So, molecule have higher molar mass has comparatively high boiling point.Example: An alcohols with lower hydrocarbon chain has lower boiling points as compare to alcohols with higher  hydrocarbon chain.

To learn more about alcohols,

https://brainly.com/question/4698220

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WILL GIVE BRAINLIEST TO FIRST ANSWER
picture ⬆️

Answers

Answer: A Molecular Approach, 2/e. Mole ratio. 2 mol C4H10 : 13 mol O2 : 8 mol CO2 : 10 mol H2O. ❖ 2 molecules of C4H10 react ... of aluminum and 73.15 g of sulfur are combined to form aluminum sulfide according to the equation:.

Explanation:

Hydration of alkynes gives good yields of single compounds only with symmetrical or terminal alkynes. Draw the major organic product(s) formed when 3-methylcyclodecyne undergoes hydration in the presence of HgSO4 and H2SO4.

Answers

Answer:

Following are the solution to the given choice:

Explanation:

Hex-2-yne is just not alkyne symmetric, therefore two things respectively hexan-3-one and hexan-2-one are to be given.

The attached file it displayed the response along with the mechanism, please find the.

Now we need to find the amount of NF3 that can be formed by the complete reactions of each of the reactants. If all of the N2 was used up in the reaction, how many moles of NF3 would be produced

Answers

The question is incomplete, the complete question is:

Nitrogen and fluorine react to form nitrogen fluoride according to the chemical equation:

[tex]N_2(g)+3F_2(g)\rightarrow 2NF_3(g)[/tex]

A sample contains 19.3 g of [tex]N_2[/tex] is reacted with 19.3 g of [tex]F_2[/tex]. Now we need to find the amount of [tex]NF_3[/tex] that can be formed by the complete reactions of each of the reactants.

If all of the [tex]N_2[/tex] was used up in the reaction, how many moles of [tex]NF_3[/tex] would be produced?

Answer: 1.378 moles of [tex]NF_3[/tex] are produced in the reaction.

Explanation:

The number of moles is defined as the ratio of the mass of a substance to its molar mass.

[tex]\text{Number of moles}=\frac{\text{Given mass}}{\text{Molar mass}}[/tex]       ......(1)

Limiting reagent is defined as the reagent which is completely consumed in the reaction and limits the formation of the product.

Excess reagent is defined as the reagent which is left behind after the completion of the reaction.

In the given chemical reaction, [tex]N_2[/tex] is considered as a limiting reagent because it limits the formation of the product and it was completely consumed in the reaction.

We are given:

Mass of [tex]N_2[/tex] = 19.3 g

Molar mass of [tex]N_2[/tex] = 28.02 g/mol

Putting values in equation 1:

[tex]\text{Moles of }N_2=\frac{19.3g}{28.02g/mol}=0.689mol[/tex]

For the given chemical reaction:

[tex]N_2(g)+3F_2(g)\rightarrow 2NF_3(g)[/tex]

By the stoichiometry of the reaction:

1 mole of [tex]N_2[/tex] produces 2 moles of [tex]NF_3[/tex]

So, 0.689 moles of [tex]N_2[/tex] will produce = [tex]\frac{2}{1}\times 0.689=1.378mol[/tex] of [tex]NF_3[/tex]

Hence, 1.378 moles of [tex]NF_3[/tex] are produced in the reaction.

The student kicks the soccer ball three times and I shouldn’t record the distance the amount of time the ball travels and the average speed in the table shown below soccer ball girl how many seconds did it take for the ball to travel 30 m during kick three

Answers

30 time 3 60 so that’s the answer it’s

Answer: D it’s is 2.0 s

i think..

Explanation:

The MO diagram of CN is similar to N2. Based on the molecular orbital energy-level diagram of CN, which of the following statements are correct?

i. The CN bond order is 2.
ii. CN is diamagnetic.
iii. The bond enthalpy in CN- ion is higher than CN.
iv. CN has longer bond length than CN-

answer choices:
a. ii, iv
b. iii, iv
c. i, iii
d. i, ii
e. i, iv

Answers

Answer:

Nitrogen molecule (N2)

The electronic configuration of nitrogen (Z=7) = 1s2 2s2 2px12py12pz1.

The total number of electrons present in the nitrogen molecule (N2) is 14.

In order to maximize energy, these 14 electrons can be accommodated in the different molecular orbitals.

N2: KK'(σ2s)2 (σ*2s)2 (π2Px)2 (π2py)2 (σ2pz)2

Here (σ1s)2 (σ*1s)2 part of the configuration is abbreviated as KK’, which denotes the K shells of the two atoms. In calculating bond order, we can ignore KK’, as it includes two bonding and two antibonding electrons.

The bond order of N2can be calculated as follows:

Here, Nb = 10 and Na = 4

Bond order = (Nb−Na) /2

B.O = (10−4)/2

B.O = 3

So your answer should be C3.

THIS NOT MY WORK. its for my sister. HELP HER

Answers

Answer:

Your answer will be b(molten material from the outer core makes its way to the surface of earth)

Explanation:

Answer:

C

Explanation:

I'm pretty sure molten material comes from the inner core. I think you can search up this though, try to find where molten material comes from. Goodluck!

Calculate the volume of 0.07216 M AgNO3 needed to react exactly with 0.3572 g of pure Na2CO3 to produce solid Ag2CO3.

Answers

Answer:

93.4 mL

Explanation:

Let's state the reaction:

2AgNO₃ +  Na₂CO₃  →   Ag₂CO₃  +  2NaNO₃

We determine the moles of sodium carbonate:

0.3572 g . 1mol / 105.98g = 3.37×10⁻³ moles

Ratio is 1:2. We say:

1 mol of sodium carbonate react to 2 moles of silver nitrate

Then, our 3.37×10⁻³ moles of carbonate may react to: 3.37×10⁻³ . 2

= 6.74×10⁻³ moles

If we convert to mmoles → 6.74×10⁻³ mol . 1000 mmol / mol = 6.74 mmol

Molarity is mol/L but we can use mmol /mL

6.74 mol / volume in mL = 0.07216 M

6.74 mol / 0.07216 M = volume in mL → 93.4 mL

I dont understand this

Answers

Answer:

me too

Explanation:

The last one Metallic bonding

About how long did it take for this population to reach the carrying capacity of this habitat

Answers

Answer:100

Explanation:

Look at the reaction below. Upper H subscript 2 upper S upper O subscript 4 (a q) plus upper C a (upper O upper H) subscript 2 (a q) right arrow upper C a upper S upper O subscript 4 (a q) plus 2 upper H subscript 2 upper O (l). Which substance is the base in the reaction? A. 2H2O (l) B. H2SO4 (aq) C. CaSO4 (aq) D. Ca(OH)2 (aq)

Answers

Answer:

Its D  

Explanation:

Did it on edge

According to the reaction: H₂S0₄ + Ca(OH)₂ → CaSO₄+ 2H₂O, the base is Ca(OH)₂.

What is a base?

Base is defined as a substance which can accept protons or any chemical compound that yields hydroxide ions (OH-) in solution.

Generally, base are substances that produces hydroxide ion when dissolve in water.  

Base are substances that reacts with acid to produce salt and water. Example of base includes sodium hydroxide, potassium hydroxide, magnesium hydroxide, calcium hydroxide etc.

Therefore, according to the reaction:

H₂S0₄ + Ca(OH)₂ → CaSO₄+ 2H₂O

The base is Ca(OH)₂.

learn more on chemical reaction here: https://brainly.com/question/14575718

A potted plant is placed under a grow lamp, which provides 6,400 J of energy to the plant and the soil over the course of an hour. The specific heat capacity of the soil is about 0.840 J/g°C and the temperature goes up by 9.25°C of soil. How many grams of soil are there?

Answers

Answer:

823.7g

Explanation:

Using the formula as follows:

Q = m × c × ∆T

Where;

Q = amount of heat (J)

m = mass of substance (g)

c = specific heat capacity (J/g°C)

∆T = change in temperature (°C)

Using the information given in this question as follows:

Q = 6,400 J

m = ?

c of soil = 0.840 J/g°C

∆T = 9.25°C

Using Q = mc∆T

m = Q ÷ c∆T

m = 6,400 ÷ (0.840 × 9.25)

m = 6400 ÷ 7.77

m = 823.7g

Answer:

There are 823.68 grams.

Explanation:

Calorimetry is responsible for measuring the amount of heat generated or lost in certain physical or chemical processes.

Between heat and temperature there is a relationship of direct proportionality. The constant of proportionality depends on the substance that constitutes the body and its mass, and is the product of the specific heat by the mass of the body. In summary, the amount of heat Q that receives or transmits a mass m of a substance with specific heat C to raise its temperature from T1 to T2 is given by the formula:

Q= C*m* (T2- T1) = C*m* ΔT

In this case:

Q= 6400 JC= 0.840 [tex]\frac{J}{g*C}[/tex]m= ?ΔT= 9.25 C

Replacing:

6400J= 0.840 [tex]\frac{J}{g*C}[/tex] *m* 9.25 C

Solving:

[tex]m=\frac{6400 J}{0.840 \frac{J}{g*C} *9.25 C}[/tex]

m=823.68 grams

There are 823.68 grams.

In which substance are molecules moving the fastest? The options are, a. Solid water (ice) b. Liquid water c. Water vapor (gas)

Answers

Answer:

The answer is C

Explanation:

The more space the molecules have the faster they will move, solid doesn't allow movement at all, when it gets to liquid they are free to move around because it's more space, when a gas they can move all around in the air.

If atoms are electrically neutral then how do they form bonds?​

Answers

Answer:

The nucleus contains neutrons and protons; protons carry the positive charge. The shells contain electrons which carry the negative charge. So, how can the atom be changed. ... Covalent bonds are electrically neutral because they are not formed due to the transfer of electrons but sharing of electrons.

Explanation:

Hope it helps ^-^

#CarryOnLearning

Answer:

When an atom has an equal number of electrons and protons, it has an equal number of negative electric charges (the electrons) and positive electric charges (the protons). The total electric charge of the atom is therefore zero and the atom is said to be neutral. ... Chemically, we say that the atoms have formed bonds

Consider the reaction 4FeS2 + 11O2 → 2Fe2O3 + 8SO2. If 8 moles of FeS2 react with 15 moles of O2, what is the limiting reactant? (3 points)


SO2

O2

Fe2O3

FeS2

Answers

Answer:

O2

Explanation:

for find the limiting reactant you must calculate the moles of the reactants from the amount that you have and from the MM:

MM FeS2 = 120n  = 26.2g / 120g/mol = 0,218 mol

MM O2  = 32n = 5,44g/32g/mol = 0,17 mol

The limiting reactant is  

O2

Which of these is NOT a producer in the coral reef ecosystem? *
1. phytoplankton
2. sea grass
3. microalgae
4. coral

Answers

Answer:

option number 4. 'coral'

Explanation:

-

A 136 g sample of an unknown substance was heated from 20.0 °C to 40.0 °C. In the process the substance absorbed 2440 J of energy. What is the specific heat of the substance? Identify the substance among those listed in Table 2.

A. the specific heat is 0.897 J/g.C, The Substance is aluminum
B. the specific heat is -0.897 J/g.C, The Substance is aluminum
C. the specific heat is 4.184 J/g.C, The Substance is water
D. there's not enough information to determine which is the substance.

Answers

Answer:

Option A. The specific heat is 0.897 J/gºC, The Substance is aluminum.

Explanation:

We'll begin by calculating the change in temperature of the substance. This can be obtained as follow:

Initial temperature (T₁) = 20.0 °C

Final temperature (T₂) = 40.0 °C

Change in temperature (ΔT) =?

ΔT = T₂ – T₁

ΔT = 40 – 20

ΔT = 20 °C

Finally, we shall determine the specific heat capacity of the substance. This can be obtained as follow:

Mass (M) = 136 g

Change in temperature (ΔT) = 20 °C

Heat (Q) absorbed = 2440 J

Specific heat capacity (C) =?

Q = MCΔT

2440 = 136 × C × 20

2440 = 2720 × C

Divide both side by 2720

C = 2440 / 2720

C = 0.897 J/gºC

Comparing the specific heat capacity (i.e 0.897 J/gºC) of the substance with those in the table above, the substance is Aluminum.

Thus, option A gives the correct answer to the question.

A chemical bond formed when two atoms share three pairs of electrons is a ________ bond; it is best described as ________.

Answers

Answer:

Explanation:

A chemical bond formed when two atoms share three pairs of electrons is a triple bond; it is best described as covalent.

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