Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish, and you incubate them with an FM dye for a period of time before washing the cells with excess medium to remove the dye.a)You find that if you stimulate the neurons to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect.b)After you have generated fluorescent synaptic regions by this procedure, suppose that you trigger further action potentials while continuing to wash with excess medium. Would you expect the synaptic regions to lose fluorescence

Answers

Answer 1

Answer:

a) Emission observed is due to emission of synaptic vessels

b) Yes

Explanation:

a) Proposing an explanation

The emission observed ( the synaptic regions of the neurons remaining fluorescent ) is due to the emission on the synaptic vessels given that FM dyes have lipid-sensitive emission which can be loaded into a recycled synaptic vesicle

b)

The synaptic regions will lose fluorescence when you continue to wash with excess medium, also the more action potentials triggered the more observed emission. hence the increase in the loss of fluorescence is due to the fact that the emissions are not permanent


Related Questions

what kind of graph is this?​

Answers

Answer:

Explanation:

financial chart

Financial chart:):):):););););)

!!20 POINTS!!
Human use a (an) ____________ circulatory system to remove waste.

A. Open
B. Closed
C. Capillariy
D. Continuous

Answers

Answer:

its continuous

BRAINLIEST
PLZZ HELP ME !!!

Answers

Answer:

the answer is in picture

5 factors that influence price​

Answers

Explanation:

Price Determination: 6 Factors Affecting Price Determination of Product

Product Cost: The most important factor affecting the price of a product is its cost. ...

The Utility and Demand: ...

Extent of Competition in the Market: ...

Government and Legal Regulations: ...

Pricing Objectives: ...

Marketing Methods Used:

The enzyme dihydrofolate reductase (DHFR) normally resides in the cytosol, and it can be imported into mitochondria by appending a mitochondrial signal sequence. However, when this modified DHFR is incubated with methotrexate, which is a substrate analog that binds tightly to the active site, the modified DHFR is no longer imported. Propose an explanation for this finding.

Required:
a. Consider a transmembrane protein that spans the inner nuclear membrane. Suggest a pathway by which such a protein could reach its destination.
b. Clathrin can assemble with multiple types of adaptors to create coated vesicles. Explain why this multiplicity of adaptors is useful to the cell

Answers

Answer:

Within the mitochondria, the import takes place post-translationally. The imported protein must be in an unfolded condition so that it can go through the mitochondria's import machinery. In the existence of methotrexate, the bound DHFR moiety remains in the folded state, and thus, stays on the surface of mitochondria.

It is witnessed that the presequence must be of the length of 55 amino acids to cover both the membranes of mitochondria and must be of about sixty amino acids length to associate with the mhsp70. If the presequence of this methotrexate bound DHFR is of seventy-five amino acids in length, it can associate with mhsp70 within the matrix, to initiate the ATP reliant import within the mitochondria. Due to the presence of various endocytic and intracellular sorting signals, multiple adaptors are essential.

Certain species of blind fish living in perpetual darkness in the ocean depths have evolved from ancestors that could see. Research indicates that the brain centers for vision in these fish is degenerating, whereas the brain centers controlling other senses are enlarging. What might be the MOST probable reason for the loss of vision in these fish

Answers

Answer:

Explanation:

Evolution for lack of light. If there is no reason for it you lose the ability but it strengthens other senses. It's compensation. The need to tell if the temperature has increased because of something nearby or ability to feel miniscule movement will increase to compensate for that lack of sense.


In the following choices, the one that is NOT a function of the skeletal
system is
O protect organs
make red blood cells
transport blood cells
O provide shape and support

Answers

Answer:

I'm pretty sure it's transport blood cells

For the following question, match the key event of meiosis with the stages listed below. I. Prophase I V. Prophase II II. Metaphase I VI. Metaphase II III. Anaphase I VII. Anaphase II IV. Telophase I VIII. Telophase II Centromeres of sister chromatids disjoin and chromatids separate.

Answers

Answer:

VII. Anaphase II

Explanation:

During metaphase II, fibers of the spindle apparatus drive chromosomes to the cell equatorial plane, where they line up. Sister chromatids are holden together until they reach Anaphase II, during which specialized enzymes break the bonds between chromatids and separate them. Each chromatid migrates to one of the poles. In telophase II, the new chromosomes are already in the corresponding poles, and the nuclear membrane forms again in each pole

21. Which force is used to separate solid pollutant particles from smoke given out from factories?
onlar​

Answers

Answer: Electrostatic Force

Explanation:

Electrostatic force is used to separate solid pollutant particles from smoke given out from factories

Help please

What factors can influence the carrying capacity of an ecosystem?(CHOOSE ALL THAT APPLY)
A The number of predators
B The amount of available food
C The amount of water
D The amount of sunlight
E The water clarity of an aquatic ecosystem

Answers

Answer:

ABCD

Explanation:

All of the above except e I think

5. What do Chloroplast and Mitochondria have in common?
A. They contain DNA
B. Provide energy to the cell
C. Are in charge in cell transport
D. Make proteins

Answers

Answer:

B. Provide energy to the cell

Explanation:

Both the chloroplast and the mitochondrion are organelles found in the cells of plants, but only mitochondria are found in animal cells.

The function of chloroplasts and mitochondria is to generate energy for the cells in which they live.

The structure of both organelle types includes an inner and an outer membrane

Which of the following is NOT an
ecological effect of mining?
A. habitat disruption
B. loss of biodiversity
C. acid mine drainage
D. increased biodiversity

Answers

d is correct

..................

^_^

One way to think about the importance of biodiversity is simply to think in terms of natural capital: the roles that genes, species, ecosystems, and ecological processes play in supporting human society and a healthy biosphere. What are benefits provided by biodiversity?

Answers

Answer and Explanation:

Biodiversity composes of different species living in a certain restricted area. It is the variability of living beings in a natural environment, involving all the species on the planet and their genetic material. This variety includes all plants, animals, fungus, and microorganisms living in a certain place, their genetic variability, and the ecosystems these species form.

Ecosystems include all the biotic, physical, and chemical components of nature, continuously interacting and depending on each other. The ecosystem is composed of living and non-living things that interact and depend on each other. These are the biotic and abiotic factors.

Biodiversity, in equilibrium with abiotic factors, provides ecosystem services, which are important for the maintenance of all forms of life, including human beings.  

Ecosystem services

When talking about ecosystem services, we are referring to the benefits that ecosystems provide us. There are four types of ecosystem services:

Provision or supply:

These are the products obtained by nature to be used or consumed, directly or with previous processing. Among them, we might mention food, clear freshwater, fertile soils, geotic materials, raw materials (that can be used for clothing, construction), renewable fuels, biochemical compounds, pharmacological/medicinal resources, among others.

Regulation:

Refers to ecological processes that improve life. Among others, we might mention climate regulation, CO² absorption, water purification, air purification, erosion control, natural disaster avoidance such as flooding, soil fertility maintenance, waste recycling, pests/plagues control, etc.

Cultural:

Refers to not the material benefits. Ecosystems provide a recreational place or area to spend time and learn. They provide a spiritual/religious space, are a source of inspiration. Ecosystems are cultural patrimony and the source of scientific knowledge.

A scientist discovers a new organism and is trying to classify it. Which observations would most likely indicate that
the organism is an animal?

It is multicellular and can move itself from place to place,

It is heterotrophic and unicellular.

It makes food from sunlight and is eukaryotic.

It is prokaryotic and can move itself from place to place.

Answers

Answer:

B) It is heterotrophic and unicellular.

Hope This Helped!

Answer:

It is multicellular and can move itself from place to place.

Explanation:

i took the test the other answer is wrong

In humans, hemophilia is a sex-linked recessive trait. If a male who has hemophilia marries a female with
normal blood clotting, what percent of the males will have the disease
A- 0 males= 0% of the males
B- 1 male= 50% of the males
C- 2 males= 100% of the males
D- 3 males= 100% of the males

Answers

C- 2 males. 100% of males. I don't know but try using a punnet square to figure it out yourself :)

g In transcription, the 3'-hydroxyl group of the growing RNA strand: Group of answer choices NONE OF THESE binds to the 5' ribose. attacks the outermost phosphorous group of the incoming nucleotide. ALL OF THESE attacks the 5' hydrogen of the incoming nucleotide. interacts with a positively charged metal ion in the active site.

Answers

Answer:

attacks the outermost phosphorous group of the incoming nucleotide.  

Explanation:

Transcription is the biological process where RNA is formed. As you may already know, RNA is an extremely important nucleic acid for genetic processes and the production of proteins. The transcription has three steps that are called start, elongation and end. In the elongation phase, the RNA strand is growing, at this time, RNA polymerase, which is the enzyme responsible for RNA elaboration, places the nucleotides, in the RNA strand, in the 5' - 3' direction. This enzyme causes the hydroxyl, which makes up the nucleotide at the 3' end of the forming RNA, to attack the phosphorus present in the incoming  ribonucleotide.

Beatrice’s science teacher gave her an unknown substance to identify based on its properties. After a few tests, Beatrice listed her observations in a chart.

reflects light very well
warms up in my hand
flattens out when hit with a hammer
conducts electricity

Which kind of substance does Melinda have?
A. metalloid
B. nonmetal
C. metal
D. noble gas

Answers

b. nonmetal i just took it

BRAINLIEST
CAN SOMEONE PLZ HELP ME!!

Answers

Explanation:

I’m pretty sure it’s phenotypes

convention cells hbnmb,

Answers

Answer:

convention cells

Explanation:

In the field of fluid dynamics, a convection cell is the phenomenon that occurs when density differences exist within a body of liquid or gas. These density differences result in rising and/or falling currents, which are the key characteristics of a convection cell. When a volume of fluid is heated, it expands and becomes less dense and thus more buoyant than the surrounding fluid. The colder, denser part of the fluid descends to settle below the warmer, less-dense fluid, and this causes the warmer fluid to rise. Such movement is called convection, and the moving body of liquid is referred to as a convection cell. This particular type of convection, where a horizontal layer of fluid is heated from below, is known as Rayleigh–Bénard convection. Convection usually requires a gravitational field, but in microgravity experiments, thermal convection has been observed without gravitational effects.


What is an element/compound that is
cycled through ecosystem?

Answers

Answer:

Nitrogen, carbon, and phosphorus are just a few

Explanation:

How many protons, neutrons, and electrons are found in a neutral atom of lithium-8?

Answers

Answer:

Three Protons

Four neutrons

Three electrons are found in a neutral atom of lithium 8

approximately how far away is the epicenter for an earthquake if the p-waves arrive three minutes 20 seconds before the S Wave​

Answers

Answer:

Only P-waves were recorded at seismic station C because P-waves travel At different avival times

Explanation:

....

What is the answer to number 1

Answers

Answer:

TTT GGC AGT

Explanation:

In screenshot! answer quickly please

Answers

Answer:

umm i would answer but i cant see the photo whats the question?

Explanation:

Why consist of a cell body that contains the 

Answers

Answer:

The cell body, also called the soma, is the spherical part of the neuron that contains the nucleus. The cell body connects to the dendrites, which bring information to the neuron, and the axon, which sends information to other neuron

Explanation:

Mark brainliest pls

An enzyme that breaks DNA, dispels the tension, and reseals the strand ahead of a DNA replication growing fork is called a(n):

Answers

Answer:

Topoisomerase

Explanation:

Topoisomerases are enzymes that produce changes in the topology of the DNA during replication, transcription, traduction, or reparation processes. They can cut one or both strands and in order to relieve torsional stresses in the supercoiled structure of DNA. With this, they help to maintain the chromosome's integrity. There are two types of topoisomerases: topoisomerase I (it cuts only one strand of DNA) and topoisomerase II (it is able to cut both strands of DNA).

A fruit fly of genotype B R/b r is testcrossed with b r/b r. In 84 percent of the meioses, there are no chiasmata between the linked genes; in 16 percent of the meioses, there is one chiasma between the genes. What proportion of the progeny will be B r/b r

Answers

Answer:

4 percent (4%)

Explanation:

A single crossover occurs between two (non-sister) chromatids belonging to homologous chromosomes. In this case, 16 percent of the meioses have a single crossover, thereby it will produce 8 percent of the chromosomes with the original (parental) combination in the progeny and the remaining 8 percent should be recombinants. From this result, it is reasonable to conclude that half of these recombinants should be 'Br' (and the other remaining 4 percent should be recombinants 'bR'), and therefore the answer is 4 percent (4%).

Which of the following is the most susceptible to damage by pollution in the air and
precipitation

A. The roots of trees
B. Trees at high elevations
C. Birds that migrate seasonally
D. Plants that grow in lakes or rivers

Answers

Answer:

B) Trees at high elevations

Explanation:

The atmospheric conditions and precipitation toxicity is most likely to affect trees at high elevations more so than the other answers.

The tree leaves, which are the main key to the tree's photosynthesis, absorb the oxygen and Co2 from the air, which are converted into nutrients. Without proper nutrients, the trees may die out.

The North Slope of Alaska has concentrations of air pollutants comparable to the air pollutant concentration of some major cities on the east coast of the United States because

Answers

Answer:

pollutants are transported by are from Eurasia

Explanation:

Advantages and disadvantages of physical barriers in the immune system

Answers

Explanation:

advantages:Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

Physical barriers play an important role in preventing microbes from reaching tissues that are susceptible to infection. At the cellular level, barriers consist of cells that are tightly joined to prevent invaders from crossing through to deeper tissue.

What is immune system?

Immune system is a large network of organs, white blood cells, proteins (antibodies) and chemicals. This system works together to protect you from foreign invaders (bacteria, viruses, parasites, and fungi) that cause infection, illness and disease.

Disadvantages of physical barriers in the immune system is causing immuno deficiency disease.

For more information regarding immune system, visit:

https://brainly.com/question/234802

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