Experiment 2.) Pidge is able to clone each GLOW gene and express these proteins in yeast. She decides to test the function of the genes in the pathway based on their protein function in vitro. Pidge has generated a primary antibody to GLOW1. Pidge wants to demonstrate that the GLOW1 protein and physically interact with the GLOW2 and GLOW4 proteins. Question 9b.) What in vitro test can she perform? (3pts)

Answers

Answer 1

Answer:

Co-immunoprecipitation

Explanation:

Co-immunoprecipitation is a bio-technique use to analyze the physiological relevance of protein to protein interactions by binding the protein with special antibodies. With this vitro-test, a sample of a protein substance can be isolated and concentrated from thousands of other proteins.


Related Questions

What two factors play a role in the type of snow flake created?

Answers

Answer:

Two factors play key roles in determining the shape of a snowflake: temperature and humidity.

Explanation:

What does it mean for a model to be "to scale", or a scaled model?

Answers

Answer:

To scale means it's the same size as in real life, as in 1 foot = one foot

Scaled means it's shown either larger or smaller, as in 1 foot = 1 inch or 1 foot = 1 mile

Explanation:

Compact bone contains cylinders of calcified bone called ______________________. These cylinders consist of 4 to 20 Compact bone contains cylinders of calcified bone called concentric rings of bone known as:___________________.
a. osteons, canaliculi
b. lamellae, canaliculi
c. osteons, lamellae
d. Haversian canals, lamellae
e. none of the above

Answers

Answer:

The correct answer is c. osteons, lamellae

Explanation:

An osteon is the basic functional unit of the cortical bone of a tubular bone. An osteon consists of a central canal that contains two vessels of Havers. Concentric bony laminae are arranged around this canal. These lamellae consist of bone cells and the surrounding base substance. Between the Havers canals there are Volkmann canals that connect the Havers canals. The construction of the cortical bone in these functional units has not yet been fully clarified. In general, it is assumed that these structures serve primarily to deliver tissue and signal transduction. This structure is also supposed to serve the stability and adaptability of the bone.

HURRY I WILL GIVE BRAINLIEST AND VERIFIED ANSWER & 100 PTS Earthworms are a species of worms that lives in moist soil along with other organisms like tiny insects and bacteria. Earthworms obtain water and nutrients by eating dead plant material found in the soil. Earthworms also release waste materials that help enrich the soil. Based on this information, which sentence is correct? The earthworms are an environment. The earthworms are a community. Soil is the earthworm’s habitat. Soil is the earthworm’s niche.

Answers

Answer:

it has to be the last and 2nd last one of them

Explanation:

Sorry if this is wrong

Badgers are a type of mammal that eats mostly earthworms and insects. They sometimes eat small mammals, birds, and reptiles, as well as plant roots and fruit.

What kind of teeth would you expect a badger to have?
some flat and some round
all flat
some sharp and some flat
all sharp

Answers

Answer:

I wouldn't think badgers teeth are all that sharp so my answer is some sharp and some flat.

Answer:

its C

Explanation:

u can look up a picture of their teeth

Please help me ?!!!!

Answers

Answer:

the last one I am guessing

Explanation:

If right can you give me brainiest

Hope you have a wonderful day:)

After being hunted to near extinction, wolves were reintroduced to Yellowstone National
Park in 1995. After two years, the population of wolves increased rapidly until 2009.
After 2009, the population decreased slightly and has remained at an average of 93
wolves. Which type of growth curve describes the Yellowstone wolves?
A. population density curve
B. exponential growth curve
C. bell curve
D. logistic growth curve

Answers

Answer: B: exponential growth curve

Explanation: An exponential growth curve is J-shaped. Exponential growth is more common in R-selected species, which have a short life span and a high rate of reproduction. Yellowstone National Park has 124 wolves living in it.

How old do paleontologist believe the earth is?

Answers

Answer:

Scientists now know the Earth is actually 4.54 billion years old, an age built on many lines of evidence from the geologic record.

Explanation:

Scientists now know the earth

Drag each tile to the correct box.
Sort the types of energy according to the order in which they move through this system:
Water stored in dams falls freely with the force to make a turbine move. The turbine moves and produces electricity. This electric energy is
supplied to homes. At home, we use electricity for heating, cooling, and lighting.
Put these in order:

Electrical energy

Light energy

Potential energy

Kinetic energy

Answers

Answer:

Electrical energy > kinetic energy > light energy > potential energy

Explanation:

A pathogen that feeds off and causes harm to other organisms is called what?

Answers

There are 5 main types of pathogens that can harm other organisms and cause diseases. They are viruses, bacteria, fungi, Protozoa, and worms.
Its called parasites. Parasites are living organisms that feeds off on other organism that causes harm

Which best describes the process of evaporation/transpiration in the
water cycle?
O A. Water from the ocean enters the atmosphere as water vapor.
O B. Water on the surface of Earth enters the groundwater supply.
C. Water vapor in the atmosphere returns to the surface of Earth as
liquid water.
O D. Water on the surface of Earth and water lost by plants enters the
atmosphere as water vapor.

Answers

Answer:

A

Explanation:

Answer:

The answer is D

Explanation:

Water on the surface of Earth and water lost by plants enters the atmosphere as water vapor

The person above me is wrong I tried it and it was wrong and I tried D and I got it correct

respiration in echinoderms​

Answers

In general, echinoderms typically respire by simple diffusion, using gills or specialized projections, like tube feet or pockets, to circulate water and oxygen through their bodies. Many echinoderms also use a simple hemal system, a series of pockets and tubes that serves almost like a net of veins and arteries.

AUG is generally a start codon. what is the amino acid that AUG codes for ?

Answers

AUG codes for methionine.

(FW.02]The diagram below shows a portion of the water cyle.
Y
Spring
Aquifer
What does Y most likely represent?
O precipitation
Orecharge
Oatmosphere
Ogroundwater

Answers

Answer:

I would have to say recharge of the aquifer by using ground water after some precipitation.

Explanation:

Melting snow or rain increases ground water that percolates down to replenish the aquifer.

Elevation changes causes the aquifer to flow up in low spots/springs on the surface.  And the cycle repeats through evaporation and plant respiration.

Chemicals used in chemotherapy treatments to fight cancer can also harm cells in hair follicles and bone marrow.
What characteristic do these cels most likely share with cancerous cells?
O They form tumors.
O They spread through the blood.
• They divide rapidly.
O They contain a mutation.
A

Answers

Answer:

They divide rapidly

Explanation:

Some normal cells such as hair follicles cells, mouth cells, digestive tract cells, and bone marrow cells divide rapidly. In mammalians, hair follicle cells are cells localized in the skin, specifically in the dermal layer of the skin. In this region, epidermal cells that form the root of the hair divide rapidly by interacting with neuropeptides, hormones, and immune cells. On the other hand, the bone marrow is a spongy tissue found in bones that contain stem cells (which also divide rapidly). In consequence, it is well-known that high-dose chemotherapy treatments may damage these types of cells.


Would the movement of oxygen through the cells?

Answers

Answer - The oxygen molecules move, by diffusion, out of the capillaries and into the body cells. While oxygen moves from the capillaries and into body cells, carbon dioxide moves from the cells into the capillaries. Carbon dioxide is brought, through the blood, back to the heart and then to the lungs.

PLEASE HELP ME ITS FOR 17 POINTS!!!!!!!!!!!!!!!!!!!!

A forest area is being used for a new housing development. Most of the trees have been removed to make room for the
new houses. Predict the impact that this will have on the birds in the area.
The bird populations will stay the same because humans love birds.
The bird populations will decrease because humans see them as pests.
© The bird populations will increase because humans do not interfere with wild animals.
The bird populations will decrease due to competition for resources such as food and nesting sites.

Answers

Answer:

The bird populations will decrease due to competition for resources such as food and nesting sites.

Explanation:

Their food and habit is being taken away so they will have to compete. I hope this helps you! :)

Possible Brainliest? You don't have to if you don't want to!

Which of the following are solutions to slow down groundwater depletion?

a -Landscape with native plants

b- promote the use of water resources

c- encourage population growth

d- develop crops that require more water

Answers

It could be d- or b-

how do scientists classify species?

Answers

Answer

d because that's what they causally do

Answer:

A

Explanation:

A P E X

if some can help me with my biology final the whole 25

Answers

Answer:

add the answers if you dont know

Explanation:

Answer:

KC is looking at cells in biology class, but he doesn't know where they came from. He sees that the cells under his microscope have cell walls and chloroplasts. Therefore, KC is likely observing cell from:

A leaf that got blown onto the floor.

Plant cells are the only cells w/ chloroplast!

Explanation:

pick me as the brainliest

What is a coral reef made of?
A. Grass
B. Dead animals
C. Volcanic rocks
D. Sand

Answers

The answer is B. Dead animals

Answer: B. Dead animals

Explanation:

Coral reefs are made with calcium. How do they get this calcium? From the skeletons of various organisms in the ocean.

What TWO things cause denaturation of enzymes:

Answers

introducing heat or chemicals which will alter its pH .

When bonds are broken between atoms in molecules, energy is
a. stored as ADP
b. released
c. created
d. conserved

Answers

ANSWER: b. released

Explanation:

Which group of elements most readily combines with Group 17 elements?
a. Group 1
b. Group 17
c. Group 18
d. none, as Group 17 is the most stable group

Answers

Answer:

a. Group 1

Explanation:

Group 17 elements react most with group 1 elements because group 1 elements are metals.

HELPPPPPP ASAPPPPPPPP HELPPPP

Answers

Little to no agitation I’m pretty sure

Answer:

it is C. Little to No agintation

1. The parts of the flower involved in reproduction are ________ and __________
2. The anthers bear the ___________the microspore mother cells give rise to _______or pollen grains after meiotic cell division.

Answers

Answer:

1. Stamen and pistil

2. Pollen, microspore

Explanation:

The flower is the reproductive part of certain plants called ANGIOSPERMS. They make use of their flower for sexual reproduction. The different parts of the flower work together to achieve a common goal, which is to reproduce, however, they do it in various ways. For example, accessory parts like petals attract pollinators. The major parts of the flower that are directly involved in the reproductive act are STAMEN (male) and PISTIL (female).

The stamen which constitutes a structure called ANTHER bears the pollen grains or microspore, which is made when the microspore mother cells undergo meiotic division. Hence, the pollens are released from the anther of a male flower (stamen), into the stigma of the female flower (pistil).

You are looking at the side of a cliff with many horizontal rock layers. In the upper layers you find fossils of rodents, but in layers further below you find amphibians but no rodents. In some still-deeper layers there are neither amphibians nor rodents but there are fossils of beetles and other insects. In the deepest layers you find no insects but you do find millipedes. This fossil evidence suggests that

Answers

Answer:

millipedes were the first of these animal groups to evolve.

Explanation:

Fossils are structural remains of animals from the past. As these animals die, it takes years for the remains to be buried.

The millipedes are at the lower layer of the rock, next to insects, amphibians, and then rodents. This shows that millipedes existed and evolved before the rest of the found animal fossils.

Which wavelength of light are more stretched—red light or blue light?

Answers

red light is more stretched

Answer:

Red

Explanation:

Blue light has a shorter wavelength than red.

what is the value of 6a + 3

Answers

Answer:

3 ( 2 + 1 )

Explanation:

Plant cells usually can be distinguished from animal cells because only plant cells have

Answers

Answer:

A plant cell contains a large, singular vacuole that is used for storage and maintaining the shape of the cell. In contrast, animal cells have many, smaller vacuoles. Plant cells have a cell wall, as well as a cell membrane. In plants, the cell wall surrounds the cell membrane.

Explanation:

Brainliest please?

Other Questions
Describe what the Rosetta Stone was and why it was so important to understanding Ancient Egypt. How old do paleontologist believe the earth is? Black friday sale on TVs were 50 in tv for $148.00 or 65 in for $199.00 which is the better buy this is not worth a grade just some understanding 2+2jjjjjjkkkkkkuyuoo A sequence of a DNA template strand is shown below.3 T C C A A T G G C T T A T T T G C A 5Which of these is the correct amino acid chain produced from the DNA template strand?Arginine Leucine - Proline Asparagine Lysine ArginineSerine - Leucine Proline Asparagine Lysine ArginineTryptophan Phenylalanine Leucine Glycine Asparagine PhenylalanineTryptophan Phenylalanine Leucine Glycine Asparagine - Serine A sporting goods store sells two types of bicycles, in two colors. The tableshows its sales over the last year.Road bikeMountain bikeTotalSilver8139120Black542680Total13565200Are buying a road bike and buying a silver bike independent events?A. Yes, because P(road bike) = 0.675 and P(road bike | silver) = 0.675.B. No, because P(road bike) = 0.675 and P(road bike | silver) = 0.6.C. Yes, because P(road bike) = 0.405 and P(road bike | silver) = 0.675.O D. No, because P(road bike) = 0.675 and P(road bike | silver) = 0.675. plissssssssss....... A metal is found that has a mass of 27g and will displace 6mL of water. Calculate its density and identify the metal. was unoka the best drummer true or false during this time as ruler, Ashoka became more violent and warlike Read the excerpt below and then answer the question that follows:It all began with Effie's getting something in her eye. It hurt very much indeed, and it felt something like a red-hot sparkonly it seemed to have legs as well, and wings like a fly. Effie rubbed and criednot real crying, but the kind your eye does all by itself without your being miserable inside your mindand then she went to her father to have the thing in her eye taken out. Effie's father was a doctor, so of course he knew how to take things out of eyes.When he had gotten the thing out, he said: "This is very curious." Effie had often got things in her eye before, and her father had always seemed to think it was naturalrather tiresome and naughty perhaps, but still natural. He had never before thought it curious.Effie stood holding her handkerchief to her eye, and said: "I don't believe it's out." People always say this when they have had something in their eyes."Oh, yesit's out," said the doctor. "Here it is, on the brush. This is very interesting."Effie had never heard her father say that about anything that she had any share in. She said: "What?"The doctor carried the brush very carefully across the room, and held the point of it under his microscopethen he twisted the brass screws of the microscope, and looked through the top with one eye."Dear me," he said. "Dear, dear me! Four well-developed limbs; a long caudal appendage; five toes, unequal in lengths, almost like one of the Lacertidae, yet there are traces of wings." The creature under his eye wriggled a little in the castor oil, and he went on: "Yes; a bat-like wing. A new specimen, undoubtedly. Effie, run round to the professor and ask him to be kind enough to step in for a few minutes.""You might give me sixpence, Daddy," said Effie, "because I did bring you the new specimen. I took great care of it inside my eye, and my eye does hurt."The doctor was so pleased with the new specimen that he gave Effie a shilling, and presently the professor stepped round. He stayed to lunch, and he and the doctor quarreled very happily all the afternoon about the name and the family of the thing that had come out of Effie's eye.But at teatime another thing happened. Effie's brother Harry fished something out of his tea, which he thought at first was an earwig. He was just getting ready to drop it on the floor, and end its life in the usual way, when it shook itself in the spoonspread two wet wings, and flopped onto the tablecloth. There it sat, stroking itself with its feet and stretching its wings, and Harry said: "Why, it's a tiny newt!"The professor leaned forward before the doctor could say a word. "I'll give you half a crown for it, Harry, my lad," he said, speaking very fast; and then he picked it up carefully on his handkerchief."It is a new specimen," he said, "and finer than yours, Doctor."It was a tiny lizard, about half an inch longwith scales and wings.So now the doctor and the professor each had a specimen, and they were both very pleased. But before long these specimens began to seem less valuable. For the next morning, when the knife-boy was cleaning the doctor's boots, he suddenly dropped the brushes and the boot and the blacking, and screamed out that he was burnt.And from inside the boot came crawling a lizard as big as a kitten, with large, shiny wings."Why," said Effie, "I know what it is. It is a dragon like the one St. George killed."And Effie was right. That afternoon Towser was bitten in the garden by a dragon about the size of a rabbit, which he had tried to chase, and the next morning all the papers were full of the wonderful "winged lizards" that were appearing all over the country. The papers would not call them dragons, because, of course, no one believes in dragons nowadaysand at any rate the papers were not going to be so silly as to believe in fairy stories. At first there were only a few, but in a week or two the country was simply running alive with dragons of all sizes, and in the air you could sometimes see them as thick as a swarm of bees. They all looked alike except as to size. They were green with scales, and they had four legs and a long tail and great wings like bats' wings, only the wings were a pale, half-transparent yellow, like the gear-boxes on bicycles.Question:How would you summarize the events in the story so far? Be sure to use details from the text to support your answer. Consider this equation.7.8 + 2(0.75m + 0.4) = -6.4m + 4(0.5m - 0.8)Mia solved the equation and determined that m = 2. Is she correct?She is incorrect because when substituting 2 for m the result was a true statement. She is incorrect because when substituting 2 for m the result was a false statement.She is correct because when substituting 2 for m the result was a true statement.She is correct because when substituting 2 for m the result was a false statement. What challenge did the newly independent states face? what is a receptacle What is the range in interval notation? how many essential parts are in the plot which could be the name of a plane? PLZ HELP ASAP WILL MAKE BRAINLIEST FOR THE BEST ANSWERWhat landmark was Bartolomeu Dias the first European to reach?a) Straits of Maellanb) Northwest Passagec) tasmaniad) Cape of Good hope g) make two sentences for each of the following usingtoo and using not... enoughyoucan't drinkwine. It's expensive. if you answer this you are a genius.