Describe how restriction enzymes and ligase enzymes are used to make the plasmids that are introduced to bacteria cells during transformation. Include the words sticky ends and complementary in your response.

Answers

Answer 1

Answer:

DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments

Explanation:

Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., BamH), or blunt ends, where the resulting strands are of the same length (e.g., HaeIII). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.


Related Questions

help . ill mark u as Brain list​

Answers

Direction I think????
direction is up, net force is 2 up, and it’s balanced

Which trait/s did the common ancestor
of Lizards and Chimpanzees NOT have?

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One is related to humans and the other isn’t

Beth broke her ankle bone. The doctor gives her a brochure on foods she can eat to help restore the health of her bones. Which element will help Beth strengthen the bone?
1.carbon
2.phosphorus
3.nitrogen ​

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the answer that you are looking for is phosphorus

HELP WITH THIS PLEASE.

Answers

Answer:

nvdzusikjvbnosajLXNcuvjzcvx

Explanation:

Someone pleaseeeee help me :(((

Answers

Answer:

Oxygen

Explanation:

Plants release oxygen during photosynthesis and need light.

hi, please help me with this biology question :)

the question is attached as an image. here are the answer choices:
a. large claw allele will increase, small claw allele will decrease
b. large claw allele will decrease, small claw allele will increase
c. large claw allele will decrease, small claw allele will decrease
d. large claw allele will increase, small claw allele will increase

i'd really appreciate help asap!

Answers

Answer:

a. large claw allele will increase, small claw allele will decrease

Explanation:

Looking at the graph we can see that the number of male crabs with larger claws is increasing every time while the smaller claws are decreasing.

Which of the following best describes the cytoplasm in the cell?

a watery substance where cell processes take place
a hard substance that digests food molecules
a watery substance that keeps molecules from leaving the cell
a hard substance that keeps the cell rigid

Answers

Answer: A watery substance where cell processes take place.

Explanation: Process of elimination. The 2nd and last answer choices are both immediately wrong because cytoplasm is not a hard/solid substance. And the third answer choice is wrong because the cell membrane’s job is to prevent organelles from leaving the cell, not the cytoplasm. Therefore the answer is A, a watery substance where cell processes take place.

It is a jelly-like liquid that surrounds everything inside the cell.

A parasite _____.

is always a fungi
never causes disease
can survive on its own
needs food from another organism

Answers

I believe the correct answer is option D. needs food from another organism. (:

Answer:

needs food from another organism

Explanation:

why do we need to follow the manual handling procedures and techniques?​

Answers

Because it’s necessary

Which statements best describe the first stage of cellular respiration? Select three options


A - the stage happens in cytoplasm
B- the stage happens in mitochondria
C - Oxygen combines with the small molecules
D - Glucose is broken down
E - Energy is released

Answers

C because cellular respiration uses combine oxygen and other toxic waste.

Two cells have surface area to volume ratios of 1:1 and 5:1. Which one has the ability to move nutrients in and expel wastes the best? Explain why

Answers

The answer is 5.1 because the nucleus will work faster

*AQUATICS SCIENCE*
Q: Which image shows the most likely path of a barrel floating down a meandering river?

Answers

Answer:

C

Explanation:

A meandering stream has a single channel that snakes through its valley, so the distance 'as the stream flows' is greater than the distance 'as the crow flies.'

Image  C is  the most likely path of a barrel floating down a meandering river.

What is the meandering of a river?A meandering stream has a single channel that snakes through its valley, so the distance 'as the stream flows' is greater than the distance 'as the crow flies.' The outer edge of the water moves faster than the inner as it flows around these curves.Meanders form as a result of both deposition and erosion, and they gradually migrate downstream. Because of the decreased friction, the force of the water erodes and undercuts the river bank on the outside of the bend, where the water flow has the most energy.

To learn more about : Meanders

Ref : https://brainly.com/question/86053

#SPJ2

How is a mean established?

Answers

Answer:

1 : to institute (something, such as a law) permanently by enactment or agreement. 2 obsolete : settle sense 7. 3a : to make firm or stable. b : to introduce and cause to grow and multiply establish grass on pasturelands.

Which of the following refers to the place where a species lives?
A
niche
B
habitat
С
ecosystem
D
biome

Answers

Answer:

habitat

Explanation:

Habitat refers to the natural environment or the natural place where an organism lives. Habitat also refers to the physical place where a species or a population lives. Hence, habitat of grizzly bear refers to the physical place where it lives.

Answer: B Habitat

A habitat meets all the environmental conditions an organism(any living thing) needs to survive

PLEASE HELP!!!!
For this assignment you will be researching the different types of fungi, and reporting on their usefulness. Choose 5 different fungi and report on the following: Name of fungi What are their major characteristics? Do they have one that separates them out from others? Where do they usually grow? Pros and Cons (are they toxic? Are they beneficial to plants? Nitrogen fixers?) Give two uses that they might have

Answers

Answer:

Brown rot fungi that produce hydrogen peroxide which attacks cellulose.

Soft rot fungi that produce an enzyme called cellulase which digests cellulose.

White rot fungi that produce enzymes capable of digesting lignin.

Phycomycetes (Algal fungi)

Ascomycetes (Sac fungi)

Basidiomycetes (Club fungi)

Deuteromycetes (imperfect fungi)

here are present different types of fungi. These fungi are classified based on their morphological structure of the mycelium spore formation, fruiting bodies and mode of life cycles. The fungi are divided into five phyla such as: Chytridiomycota, Zygomycota, Glomeromycota, Ascomycota, and Basidiomycota

Explanation:

HOPE IT IS HELPFUL

Earth Cycles Deep Practice 1 Question

Answers

Answer:

Put most plainly, it's timezones.

Explanation:

Lalitha lives in India. Since it is so far away, she is in a completely different time zone than Crystal.

photosynthesis uses this form of energy to drive the process

Answers

Answer:

i think its the energy of light

Explanation:

Answer:

Light energy

Explanation:

Photosynthesis, the process by which green plants and certain other organisms transform light energy into chemical energy. During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds.

1) Base your answer on the information and data table below and on your knowledge of biology.
A student wanted to investigate the effect of light on the rate of ripening of tomatoes. She set up four pots of the same size with identical amounts of soil, water, and type of tomato plants. Each plant was exposed to a different intensity of light as shown in the table below.
The independent variable in this experiment is the


A)
light intensity

B)
amount of soil provided

C)
type of tomato plant

D)
color of tomatoes

Answers

Answer:

A) intensity of light

Explanation:

The independent variable is the thing being changed. A dependent variable is the effect caused by that change

what is the complementary DNA strand to ATCG

Answers

Answer: TAGC is the complementary DNA strand to ATCG.

Explanation: Hope this helps some.

A T C G

|   |   |   |

T A G C

what is DNA replication similar to

Answers

Answer:

i wanna say RNA

Explanation:

DNA sequencing.
I remember this from Biology Freshman year!

Coal is a fossil fuel produced from plant material underground over very long geological periods of time. What are petroleum and natural gas originally formed from?

Answers

It was formed from the remains of ancient plants and animals.

Question 8
The force exerted on you by the weight of molecules in the air is known as
Wind
Air Pressure
Radiation
Humidity
2021 Illuminate Education, Inc.

Answers

B because it’s it’s exterded by weight because wind dosent have weight it dosent move air pressure does

What's the difference between monosomy and trisomy?

Answers

Answer:

Trisomies and monosomies are two types of chromosomal abnormalities. Specifically, a trisomy is when a person has three of a particular chromosome, instead of the usual two. A monosomy is when they just have one chromosome instead of the usual two.

For example: A trisomy is down syndrome

Explanation:

Answer:

trisomy is when a person has three of the particular chromosome rather than two. Monosomy is when someone has one chromosome rather than the usual two.

What happens to the cell’s genetic information during the cell cycle?

Answers

Answer:I think During most of a cell's life cycle, DNA, along with proteins, is a mass of loose strands called chromatin (KROH-muh-tin). Before cell divion, DNA is duplicated, or copied. Then, in an early stage of cell division, the chromatin is compacted into visible structures called chromosomes (KROH-muh-sohmz).Mitosis is used to produce daughter cells that are genetically identical to the parent cells. The cell copies - or 'replicates' - its chromosomes, and then splits the copied chromosomes equally to make sure that each daughter cell has a full set.During interphase, the cell obtains nutrients, and duplicates (copies) its chromatids (genetic material). The genetic material or chromatids are located in the nucleus of the cell and are made of the molecule DNA. hope this helps have a nice night ❤️❤️❤️

Explanation:

Answer:Mitosis is used to produce daughter cells that are genetically identical to the parent cells. The cell copies - or 'replicates' - its chromosomes, and then splits the copied chromosomes equally to make sure that each daughter cell has a full set. And because During most of a cell's life cycle, DNA, along with proteins, is a mass of loose strands called chromatin (KROH-muh-tin). Before cell divion, DNA is duplicated, or copied. Then, in an early stage of cell division, the chromatin is compacted into visible structures called chromosomes (KROH-muh-sohmz).

Explanation:

help me with this if possible

Answers

Answer:

well 5 is easy its the axis

I believe the first one is false

Pls answer I will give all my points

Answers

Answer: Pretty much it's asking if it is AP cuca

Explanation:

This equation represents a biological process. In which organelle is this process completed?
C6H12O6 + O2 --> CO2 + H20 + ATP


Cell membranes

Mitochondria

Chloroplasts

Ribosomes

Answers

Answer:

Mitochondria

Explanation:

That equation is for Cellular Respiration and it takes place in the Mitochondria in the Cytoplasm

Why does ice cream placed in a plastic cooler melt slower than ice cream placed in a metal pot?


A. because metal is a better insulator than plastic


B. because plastic is a better insulator than metal


C. because metal insulates against more heat


D. because plastic conducts more heat

Answers

Answer: B

Explanation:

Because Metal is a poor conductor of heat the inside of a plastic container is insulated and the ice cream will stay cooler longer

Besides for growth, what other reason would you want a new cell
to have the same exact DNA as the initial parent cell?
SELECT AN ANSWER
-You need to replace cells that are dead or
damaged.
-You want the new cell to have the
capability to live for an infinite amount of
time (avoid death).
-You want to avoid genetic mutations or
abnormalities.
-There is no other reason besides growth.

Answers

Answer:

You need to replace cells that are dead or

damaged.I think

What is an example of a physical weathering?


A: a metal tool left outside beginning to rust
B: microscopic organisms producing compounds that break down rock
C: a rock wearing down due to acid rain
D: bits being blown off a rock by heavy winds

Answers

Answer:

D) Bits being blown off a rock by heavy winds

Explanation:

All the other answer choices are examples of chemical weathering.

Rusting is chemical weathering.

Compounds that break down rocks is chemical weathering.

Acid rain is chemical weathering.

Wind breaking rocks, however, is physical weathering.

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