Consider an experiment in which nucleic acids are separated using agarose gel electrophoresis. In the gel you load the following samples: Lane 1: a population of linearized 3000-bp double-stranded DNA (dsDNA) molecules. Lane 2: a population of circular 3000-bp double-stranded DNA (dsDNA) molecules. Lane 2: a population of circular 3000-bp double-stranded DNA (dsDNA) molecules Which population of nucleic acids would most likely migrate through the gel fastest, and in which direction through the gel will these molecules migrate?

a. The linearized dsDNA would most likely migrate faster than the circular dsDNA. All DNA molecules would migrate toward the positive end of the gel.
b. The circular dsDNA would most likely migrate faster than the linearized dsDNA. All DNA molecules would migrate toward the positive end of the gel.
c. The circular dsDNA would most likely migrate faster than the linearized dsDNA. All DNA molecules would migrate toward the negative end of the gel.
d. The linearized dsDNA would most likely migrate faster than the circular dsDNA. All DNA molecules would migrate towards the negative end of the gel.

Answers

Answer 1

Answer:

a. The linearized dsDNA would most likely migrate faster than the circular dsDNA. All DNA molecules would migrate toward the positive end of the gel.

Explanation:

Electrophoresis gel is a technique widely used in molecular biology in order to separate macromolecules (e.g., DNA, RNA, proteins) according to their size and charge. The DNA is a molecule negatively charged, thereby the DNA fragments will always move toward the positive electrode in an electrophoresis gel. In the DNA molecule, the charge-to-mass ratio remains constant, thereby DNA fragments will separate based on their size (i.e., small DNA fragments will move through the gel faster than large ones). Moreover, for the same size, linear dsDNA fragments experience less frictional resistance against the gel than circular dsDNA fragments and, therefore, linearized dsDNAs run faster.


Related Questions

Chomosomes are duplicated during

Answers

Answer:

Chomosomes are duplicated during interphase

Brainliest?

Chromosomes are duplicated during the S phase of interphase.

This need to be present for us to be allowed to look at DNA from a hair sample
Root
Cortex
Medulla

Answers

Medulla or cortex is the answer

If more snakes moved into this environment, then
A. the number of rabbits would go up.
B.the number of grasshoppers would go up.
C. the number of toads would go up.
D. The number of hawks would go up.



NO LINKS AND DO NOT ASK A QUESTION UNDER MY QUESTION ❗❗❗​

Answers

Answer:

B. The number of grasshopers would go up.

Explanation:

A. is incorrect because it would decrease

C is incorrect  because it would also decrease

D is incorrect because snakes dont eat Hawks, but Hawks eat them

B is correct because since snakes would eat the toads, there would be no toads eating grasshoppers

Hope this helps!!!

Which type of cell division causes a puppy to grow into an adult dog?
meiosis
mitosis
eukaryotic
O homeostasis

Answers

Answer is mitosis

Meiosis is for sexual reproduction and for producing offspring, whereas mitosis is the cell division for cell reproduction so it has to be mitosis as it is the cause of cell growth, allowing the puppy to grow into an adult dog

It wouldn't be eukaryotic or homeostasis since they're not types of cell division.

Mitosis is the kind of cell division which allows a puppy to develop into an adult dog. So, the correct option is B.

What is Mitosis?

In eukaryotic cells, a process called mitosis produces two daughter cells, each of which has the same amount of chromosomes as the parent cell. Multicellular organisms, like dogs, go through a process called mitosis during which cells divide and differentiate into different cell types.

The process of meiosis, on the other hand, produces gametes with half as many chromosomes as the parent cell and is only able to occur in specialised cells, such as the ones involved in sexual reproduction. Contrarily, homeostasis has nothing to do with cell division and instead refers to the preservation of a constant internal environment within an organism.

Therefore, the correct option is B.

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Which statement best describes why accreditation is important?

a. Accreditation allows an individual to take a student loan.

b. Accreditation allows your program to charge more in tuition but gives you the edge in the job market.

c. Accreditation gives the school a better reputation, which is only important if you want a high demand job.

d. Accreditation validates the quality of the program and for some career training programs, may be
important to be considered registry eligible.

Answers

Answer: D. Accreditation validates the quality of the program and for some career training programs, may be important to be considered registry eligible.

Explanation:

Accreditation is very important in health science training programs. If the program is not accredited, you may not be able to transfer the credits to a different school and may not be eligible for your licensure or certification exam.

Answer:

Accreditation validates the quality of the program and for some career training programs, may be important to be considered registry eligible.

Explanation:

Person above is correct!

which of the following refers to the cellular interaction triggered by hormones

Answers

Answer: Interactions of Hormones at Target Cells. Hormones that act to return body conditions to within acceptable limits from opposite extremes are called antagonistic hormones.

Explanation:

Fiona wants to use the scientific method to answer the question “Do oxygen levels in the air affect rates of photosynthesis?” She reads about photosynthesis in her biology book and is ready to carry out the next step. What should she do next?
perform an experiment
design a procedure
make observations
form a hypothesis

Answers

The first thing Fiona, or anyone, should do is form her hypothesis.

The hypothesis is your own proposed explanation for whatever phenomenon you're trying to study. Think of it as a predetermined idea of what could happen based on what you already know.

Answer:

The correct answer is D!:)

Explanation:

Hope this helps!:)

3. A county has recently evolved from underdeveloped to developed and the birth and death rate have stabilized, This is known as
O A. zero population growth.
© B. birth stabilization.
© C. demographic transition.
D. death stabilization.

Answers

A county has recently evolved from underdeveloped to developed and the birth and death rate have stabilized, This is known as Demographic transition. Thus, the correct option is B.

What is Demographic transition?

Demographic transition is a phenomenon and the theory which refers to the historical shift from the high birth rates and high death rates in the societies with minimal technology, education especially of the women of the society and the economic development, to the low birth rates and low death rates in the societies with advanced technology, education and economic development of the whole society, as well as the different stages between these two scenarios.

The rise in the demand for human capital and its impact on the decline in the gender wage gap of the society during the nineteenth and the twentieth centuries period have contributed to the onset of the overall demographic transition of the society as a whole.

Therefore, the correct option is B.

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The largest volume of urine consists of what?

Answers

Answer:

The largest volume of urine consists of Urea

Why does sound not literally blow us away?

Answers

Answer:

i.d.k what?

Explanation:

List the four (4) main types of tissues and state the function of each type.

Answers

Answer:

4 basic types of tissue: connective tissue, epithelial tissue, muscle tissue, and nervous tissue. Connective tissue supports other tissues and binds them together (bone, blood, and lymph tissues). Epithelial tissue provides a covering (skin, the linings of the various passages inside the body).

Explanation:

hope this helps you

Answer:

the top person is right so yea


Density is mass divided by volume (d = m+ y). Based on the graph, what happens to a fixed mass of water when it is cooled from 4°C to 0°C?
Density of Water
1000
999.95
999.9
999.85
999.8-
Density (kg/cubic meter)
999.75-
999.7
999.65-
999.6
999.55
999.5-
0
8
10
12

Answers

Answer:

for anyone else who needs this the answer is:

A. volume increases and density decreases

Explanation:

I got a 5 on mastery test thanks to some comments on original answer

Based on the graph, the density of water decreases as the temperature decreases from 4°C to 0°C. Therefore, a fixed mass of water will have a higher volume at 0°C than at 4°C.

What is the density-temperature relationship?

The density of a substance is defined as its mass per unit volume. In the case of water, the density varies with temperature, as shown in the graph. At 4°C, the density of water is about 1000 kg/[tex]m^3[/tex]. As the water is cooled, its density decreases, reaching a minimum of about 999.87 kg/[tex]m^3[/tex] at 3.98°C (this is the temperature at which water is most dense). As the water is cooled further, the density increases again, but it remains lower than it was at 4°C. The reason for this behavior can be explained by the way water molecules behave as they are cooled. At higher temperatures, the water molecules have more kinetic energy and are more spread out, resulting in a larger volume and lower density.

Hence, the density of water decreases as the temperature decreases from 4°C to 0°C. Therefore, a fixed mass of water will have a higher volume at 0°C than at 4°C.

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PLEASE HELP QUICK!!!

If R = red-eyed and r = white-eyed, what would be the result of a cross between a white-eyed female (Xr Xr) and a red-eyed male (XRY)?

Hint: Draw a Punnet square.

A. 100% red-eyed females

B. 100% white-eyed males

C. both a and b are correct

Answers

Answer:

c. Both A and B are correct

Explanation:

Punnett squares are in the image

I’m thinking either A or C is correct

Which of the following characteristics is shared by all chemical reactions?
Produce a precipitate
Show a color change
Release heat energy
Form new substances

Answers

Answer:

Release heat energy

Show a color change

Form new substances

Explanation:

good luck

hello help pls
A glass of juice at 20°C was kept in the open. After 30 minutes, the temperature of the juice remained the same. Which statement is correct? The temperature of the surrounding air was greater than 20 °C. The temperature of the surrounding air was less than 20 °C. The temperature of the juice was equal to the temperature of the surrounding air. The temperature of the juice was less than the final temperature of the surrounding air.​

Answers

Answer:

c

Explanation:

the temperature at that very moment was equally to that of the object placed there

The diagram represents a green plant with a flower.
Base your answers to questions 62 through 64 on the diagram below and on your knowledge of science.
65 The chart belo
Identify two
organism. (
62 Describe one function of the roots. [1]
Base your
diagram repre
Halley's Come

Answers

Answer:no idea

Explanation:

What example best compares to the relationship between genes and chromosomes? PLZ Somebody help
A. A gas tank in a car because it stores energy.
B. Skin on a human because it protects the body.
C. Leaves on a tree because they provide energy.
D. A recipe in a cookbook because it gives directions.

Answers

Answer:

b

Explanation:

Answer:

D

Explanation:

I think its d

HELPP IMA GIVE YOU 20 PONTS PLISSSS

The mass extinction of the dinosaurs, approximately 65.5 million years ago, is inferred by most scientists to have been caused by


A)
a large energy surge from the surface of the Sun

B)
the occurrence of a major ice age

C)
an impact event occurring on Earth's surface

D)
earthquakes occurring along crustal plate boundaries

Answers

Answer: c

Explanation:

Please help this is a 7th grade science problem multiple choice

Answers

Answer:

Hello There!!

Explanation:

The answer is d.Nucleus.

hope this helps,have a great day!!

~Pinky~

Answer:

The correct answer is 'd'.

Explanation:

DNA is located in nucleus.

which of the organ will balance the glucose level in the body

Answers

Answer:

LIVER

Explanation:

One of the liver’s main roles in the body is controlling the amount of glucose circulating in the blood. By storing excess glucose as glycogen and creating new glucose from proteins and fat byproducts, the liver is able to maintain balanced glucose levels in your body at all times.

Answer:

The liver keeps glucose level balanced

Which would most likely be done while designing a solution?

Answers

Answer:

Steps of the technological design process include: identify a problem, research the problem, generate possible solutions, select the best solution, create a model, test the model, refine and retest the model as needed, and communicate the final solution.

Explanation:

This is a very vague question. Were there options?

The Burning of either wood or fossil fuels will add carbon dioxide to the atmosphere. How is the burning of fossil fuel different?
A. Unlike would fossil fuel require oxygen to burn
B. Unlike word fossil fuel do not require oxygen to burn
C. Unlike would fossil fuel burn without releasing energy
D. Unlike wood fossil fuels contain carbon that has been stored for millions of years

Answers

Unlike would fossil fuel burn without releasing energy

When someone says they have a "fast" metabolism, they are referring to the fact that they are able to efficiently
break down complex molecules at a higher rate than the average person.
Which of the following answer choices best describes someone with a "slow" metabolism?
Choose 1 answers
Low rate of catabolism
Low rate of anabolism
High rate of catabolism
Equal rates of catabolism and anabolism

Answers

Answer: low rate of catabolism
I hope this helps

Answer:

CORRECT (SELECTED)

Low rate of catabolism

Explanation:

Someone with a "slow" metabolism would have a low catabolic rate because their ability to break down complex molecules occurs at a slower, less efficient rate than someone with a "fast" metabolism.

How do mutations happen?

Answers

Answer:

HOPE THIS HELPS

Explanation:

A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during cell division, exposure to ionizing radiation, exposure to chemicals called mutagens, or infection by viruses.

Answer:

A mutation is a change that occurs in our DNA sequence, either due to mistakes when the DNA is copied or as the result of environmental factors such as UV light and cigarette smoke

Explanation:

A drug that prevents mircotubules from depolymerizing could be used to:_________
A) stimulate
B) inhibit mitosis
C) promote cell division
D) treat Alzheimer's disease

Answers

Answer:

A) stimulate would be the answer

Hope it helps

would occur if we were suddenly thrown back into the “Dark ages” with no use of technology

Answers

So there are a lot of people who say that the Dark Ages, lasting about 1,000 years, set humans back technologically. However, some say that since the Dark Ages really only affected Western Europe, and not the Middle East or Asia, it wouldn't make a difference.

If there had been no "Dark Ages" there would have been no Renaissance. Historians actually argue about when Renaissance started, and the answer is than it is not possible to pinpoint a date. The Crusade did more to bring the eastern world to western Europe than they did to divide.

Calculate the number and percentage of eukaryotic species that are still to be formally described?

Answers

the number and percentage of eukaryotic species that are still to be formally described is to be beauty

Eukaryotic organisms are species that have defined nucleus and membrane limited organelles. Total 11% or 250,000 are described while 97% or 8490000 are yet to be described.

What is the population of the eukaryotes?

Eukaryotes are the largest and the most dominating species that includes kingdom plants, animals, fungi and protists. A total of  8.74 million eukaryote species on Earth are present from which only 250,000 are described.

Eukaryotes are the species that are well-defined organisms that contain the genetic material enclosed within the nuclear membrane and the organelles are also enclosed.

With new discoveries and findings, many new species are described that are grouped into one of the kingdoms based on their properties and features.

Therefore, 8.74 million eukaryote species are present on Earth.

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how can blood be stored much longer​

Answers

Answer:

Whole blood can be stored at 4–8°C for up to 24 hours before the serum is separated, but it must not be frozen. Whole blood should be allowed to clot and then centrifuged at 1000 × gravitational units (g) for 10 minutes to separate the serum.

hope this helps

have a good day :)

Explanation:

Help me please omggggg ahhhhhh

Answers

Answer:

a

Explanation:

Answer:

Your answer should be B :) because the cat inherited his spots

Sorry of I am wrong!

34.Blue flower (B) are dominant. Green flowers (b) are recessive. If a male plant with blue flowers (Bb) is crossed with a female plant with blue flowers (Bb), what is the percent chance that their offspring will have green flowers? * 0% 25% 50% o 75%​

Answers

Answer:

25%

Explanation:

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