consider a negatively charged protein adsorbed on anion-exchange gel at ph 8. (a) how will a gradient from ph 8 to some lower ph be use- ful for eluting the protein?

Answers

Answer 1

The protein becomes protonated as the pH is dropped, which results in a reduction in the negative charge's intensity. Protein retention weakens over time.

Ionizable compounds are separated using ion exchange chromatography according to their overall charge. Because the charge carried by the target molecule can be easily controlled by altering buffer pH, this technique makes it possible to separate comparable sorts of molecules that would be challenging to separate by other techniques.

To separate charged biological components like proteins, peptides, amino acids, or nucleotides, ion exchange chromatography is frequently utilized. Protein is made up of amino acids, which are zwitterionic substances that have both positively and negatively charged chemical groups. Proteins can have a net positive charge, a net negative charge, or no charge depending on the pH of their surroundings. The isoelectric point, or pI, of a molecule, is the pH at which there is no net charge.

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Related Questions

based upon the experimental data, which muscle type exhibited the lowest threshold of stimulation in all conditions tested?

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based upon the experimental data, the muscle type that exhibited the lowest threshold of stimulation in all conditions tested is Lower forelimb muscle

The forelimb are the limbs at the front of the body that contain the arms and their muscles, as distinguished from the hind limbs at the rear of the body that contain the legs.

therefore, based upon the experimental data, the muscle type that exhibited the lowest threshold of stimulation in all conditions tested is Lower forelimb muscle

your question is incomplete, but most probably your full question was

Based upon your experimental data, which muscle type exhibited the lowest threshold of stimulation in all conditions tested?

a. Lower forelimb muscle

b. Upper forelimb muscle

c. Calf muscle

d. Thigh muscle

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____ media contain agent(s) that prohibit the growth of some microbes.-Differential-Enriched-Selective-All of the above

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Selective media contains agents that prohibit growth of some microbes.

Agents in selective media prevent the growth of some organisms, favoring or enabling the growth of other organisms. Many different kinds of microorganisms can thrive on differential media, but these media also contain an indicator that makes it possible to see the differences between different microbes.

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max runs a marathon in 2 hours and 40 minutes. most of the atp for this run is supplied by: group of answer choices breakdown of fatty acids anaerobic glucose breakdown aerobic glucose breakdown breakdown of amino acids

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Runners run a marathon in 2 hours and 40 minutes. Most of the ATP for this process is supplied by aerobic glucose breakdown.

Aerobic respiration reactions occur when glucose meets oxygen, then produces carbon monoxide, water, and energy. Aerobic respiration produces 36 ATP of energy, while anaerobic respiration only produces 2 ATP of energy.

The best energy supply for runners is through aerobic respiration because it requires a lot of energy to move.

The aerobic system is the primary energy system used for sprints lasting longer than three minutes. Because long-distance running is primarily limited by the delivery and use of oxygen. So, when warming up you should focus on increasing the ability of the aerobic system to supply oxygen to the muscles while running.

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which of the following statments does not describe the consequence of indecite calcimum intake

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The statement that not describe the consequence of indicate calcium intake is Low calcium intakes at some stage in the developing years limits the bones' capacity to acquire top bone mass.

A low calcium consumption at some stage in the developing years will now no longer stunt the kid's growth loss of calcium can result in a situation called 'rickets' in children. This is wherein the bones turn out to be tender and weak, they could turn out to be deformed, together with bowed legs or curvature of the spine. Rickets also can motive bone ache and bad growth.

Calcium is a mineral most customarily related to wholesome bones and teeth, even though it additionally performs an critical position in blood clotting, assisting muscle groups to contract, and regulating everyday coronary heart rhythms and nerve functions.

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review the data below from investigating life 11.1. what is the effect of the booster gene in the weedy relatives of cultivated rice?

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Transgenic weeds produce more seeds when the booster genes from cultivated rice inserted to it. the gene flow cause ecological impression

Environmental biosafety issues could arise from transgene escape by gene flow from genetically modified (GM) crops to their wild cousin species. To prevent potential outcrossing, it is advised that isolation zones be set up with enough space between GM rice varieties and wild rice populations. Additionally, GM rice that has genes that can greatly improve the ecological fitness of weedy rice shouldn't be made available in areas where it already grows abundantly and is a major concern.

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FILL IN THE BLANK. The property of an antimicrobial agent to be highly toxic against its target microbe while being far less toxic to the cells of the host organism is called _______________ toxicity.

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The property of an antimicrobial agent to be highly toxic against its target microbe while being far less toxic to the cells of the host organism is called selective toxicity.

What is toxicity?

A substance's toxicity refers to the dosage required for it to harm a living thing. When a material starts to harm an organism, it becomes toxic. In spite of common assumption, every drug has a definite level of toxicity. Even water and oxygen, in certain amounts, are harmful to living things. Furthermore, poisons affect certain species in various ways. A substance's toxicity, like that of sulfur, will differ depending on the species. Large amounts of sulfur are lethal to humans. Sulfur is an essential and pleasant ingredient for the creatures that live in the heat of volcanic vents at the bottom of the ocean, though.It was no longer accurate to forecast human toxicity levels using laboratory animals once it was realized that various poisons might affect comparable species in wildly diverse ways. To research and assess toxicity in accurate and ethical manners, new procedures and tests are being created. Toxicology is the field that studies the toxicity of various compounds.

Selective toxicity:

According to the theory of selective toxicity, a chemical may be poisonous to a pathogen but not to the infected host organism.Antimicrobial drugs like penicillin, metronidazole, and chloramphenicol work by interacting with biological components specific to their target organism. This makes it possible for the chemical to only harm cells that have the target element, such as an enzyme involved in the creation of cell walls or a specific metabolic ribozyme.

Hence, this is selective toxicity.

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Cecropias are known for the mutualistic relationship they have with which creature?.

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Cecropias are known for the mutualistic relationship they have with the Azteca ants.

A symbiotic form of mutualism between the cecropia tree and the Azteca ant. Cecropias trees provide homes for ants inside the hollow stems of the plants and also provide food rich in white carbohydrates for Azteca ant colonies.

In exchange, the ants offer protection to the cecropia tree. Ants fend off robbery from leafcutter ants, drive away locusts, and deal with much bigger threats, such as woodpeckers and monkeys. If a leaf is damaged, a chemical signal from the tree calls on the ants to investigate. Azteca ants even prune inappropriate vines

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The diagram shows a beaker of water and molecules of red dye at the beginning of an experiment (time 1) and the same beaker of water and molecules of red dye after 30 minutes (time 2). which of the following best explains what happened

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Answer: b

Explanation:

neurons are cells of the nervous system. as part of its normal function, a neuron pumps sodium ions (na ) out of the cell and potassium ions (k ) into the cell. which statement describes the role of energy in this process?

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Neurons are cells of the nervous system. As part of its normal function, a neuron pumps sodium ions (Na + ) out of the cell and potassium ions (K +) into the cell. The process requires energy that is provided by ATP.

The nervous system's cells are called neurons. The neurons use electricity to transmit information throughout the nervous system. And the differential in charges between inside and outside the cell is what creates that electricity. A difference in the cell's positive charge is where this difference in charges BEGINS. Currently, Na+ and K+ concentrations outside the cell of neurons vary due to their watery surroundings. K+ concentration is higher inside the cell than outside, but Na+ concentration is higher outside the cell than inside. However, because there are more Na+ than K+, Na+ has a tendency to enter cells while K+ has a tendency to exit them, maintaining the equilibrium of Na+ and K+ inside and outside of the cell.

The sodium-potassium pump creates by retaining K+ and discarding Na+. Because the cell is operating in opposition to the equilibrium state (equal amounts of Na+ and K+ coming in and going out), more Na+ must be expelled than must be brought in during this process (because there are more Na+ than K+). It is ion active transport.

The first step in the electric potential to transmit information through the nervous system is made possible by this difference in positive charges.

The complete question is:

Neurons are cells of the nervous system. as part of its normal function, a neuron pumps sodium ions (Na ) out of the cell and potassium ions (k ) into the cell. which statement describes the role of energy in this process?

A. The process releases energy that is used to make ATP.

B. The process releases energy in the form of heat.

C. The process requires energy that is provided by sodium and potassium ions.

D. The process requires energy that is provided by ATP.

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describe structural and functional similarities between mitochondria and chloroplasts that provide evidence of common ancestry.

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They both have double membrane, DNA etc and can generate metabolic energy. From endosymbiotic theory, Circular DNA present bacteria in mitochondria and chloroplast provide evidence of common ancestory.

Functional similarities between chloroplasts and mitochondria is to generate metabolic energy, which is evolved by endosymbiosis, they contain their own genetic systems, and both replicate by division.

Structural similarities between mitochondria and chloroplast is that they both a double membrane structure and they contain DNA, organized into nucleoi, and RNA(tRNA, mRNA, and rRNA). And both have ribosomes. Mitochondria and chloroplasts both are semiautonomous, means likely to be evolved from engulfed prokaryotes that once lived as independent organisms. Then eukaryotic cell engulfed an aerobic prokaryote, which then formed an endosymbiotic relationship with the host eukaryote, which developed into a mitochondrion or chloroplast. A prokaryote, bacteria, has circular DNA, so the mitochondria and chloroplasts have. This provides evidence of common ancestory.

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Bethany is 42 and has cervical cancer. It is probably related to an infection she had during her college years, which wassyphilis.hepatitis A.Chlamydia.human papillomavirus (HPV).

Answers

Answer:oh  dam

Explanation:

Which statement correctly describes the difference between alcohol fermentation and lactic acid fermentation?
Alcohol fermentation produces lactate, and lactic acid fermentation produces ethanol.
Alcohol fermentation is an aerobic pathway, and lactic acid fermentation is an anaerobic pathway.
Lactic acid fermentation produces lactate, and alcohol fermentation produces ethanol.

Answers

The difference between alcohol fermentation and lactic acid fermentation is lactic acid fermentation produces lactate, and alcohol fermentation produces ethanol.

Lactic acid fermentation vs alcohol fermentation

Fermentation is the metabolic process in which sugars are broken down anaerobically. This condition means in absence of oxygen.

The fermentation pathway is still related with cellular respiration process but there is an extra reaction after Glycolysis. It happens as you are making bread, beer, and doing exercise too hard. 

There are 2 types of fermentation, lactic acid fermentation and alcohol fermentation.

Lactic acid fermentation. In lactic acid fermentation, NADH transfer the electrons directly into pyruvate and produce the lactate as a byproduct. For instance, the process of making yoghurt undergo this kind of fermentation. Also, the muscle cells when you’re exercising too hard. Alcohol fermentation. In alcohol fermentation, NADH donate the electrons into pyruvate derivatives (acetaldehyde) and generating ethanol (alcohol). It needs two process from pyruvate to ethanol. 

Both of names, origin from the byproduct produced. 

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Approximately one out of every 2,500 caucasians in the united states is born with the recessive disease cystic fibrosis. According to the hardy-weinberg equilibrium equation, approximately how many people are carriers?.

Answers

A recessive trait is expressed in 1 out of every 2500 homozygous people (cystic fibrosis is inherited as autosomal recessive disease ).

How is the allele frequency in autosomal recessive determined?

The prevalence of an autosomal recessive disease in a population is q2, which in this example equals 1/2500 and q = 1/50, according to the Hardy-Weinberg Equilibrium. The two alleles (p & q) must have a frequency of 1, therefore p = 49/50 1.

How common is the allele for cystic fibrosis in the Caucasian population?

For instance, among Caucasians, the normal allele frequency is 0.975 while the mutant cystic fibrosis allele frequency is 0.025. Allele distribution across a population as a whole is impacted by this.

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Which gas is most likely to depart from the assumption of the kinetic-molecular theory that says there are no attractive or repulsive forces between molecules?.

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The gas that is most generally to depart from kinetic theory assumption is SF6 gas.

Sulphur hexafluoride – additionally called SF6 – is a 'greenhouse gas' that has lengthy performed a component in international warming, much like that of carbon dioxide (CO2).Ideal fueloline equations reason inaccuracies while used to calculate thermodynamic homes of actual gases like SF6 due to the fact actual gases do now no longer showcase perfect fueloline conduct. Real fueloline equations that appropriately mirror the thermodynamic conduct of SF6 were developed.Assumptions of the Ideal Gas Law.

The debris do not interact. There aren't anyt any appealing or repulsive forces among them. The common kinetic electricity of the fueloline debris is proportional to temperature.

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you engineer a mutation that removes the kdel sequence from a protein that is normally found in the er. where do you expect to find the mutant protein?

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Retrograde transport to a ER lumen will be used to recover a protein with such a functioning KDEL motif from the Golgi apparatus.

KDEL is indeed a target peptide sequences found on the C-terminal ending of the amino acid protein structure in humans and plants[1][2]. The KDEL sequence enables a protein's return if it is unintentionally exported from the endoplasmic reticulum (ER) as well as prevents it from being secreted. As long even as soluble resident protein has a KDEL signal sequence at its C-terminus, it will stay in the ER. Due to the dynamic nature of vesicle budding and the large level of protein in the ER, protein were unintentionally transferred to the cis-golgi through COPII coated vesicle. KDEL receptors joined to COPII & COPI vesicles aid in the transport mechanism of proteins with the KDEL signal sequence.

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the form of polycystic kidney disease (pkd) that first manifests in the early infant period is most commonly characterized as:

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The form of polycystic kidney disease that is first manifest in the early infant period is called autosomal recessive polycystic kidney disease. It is characterized as with enlarged kidney.

As the name suggests it is an autosomal recessive disease, for the disease to be inherited at least both parents should be carriers of the disease. Fetuses with this condition are characterized by enlarged kidneys. Since the kidney is enlarged it prevents the growth of lungs and makes the breathing of the fetus hard. Children with this condition are usually smaller in size than normal children because of decreased kidney and lung function. Also amniotic fluid of the mother's womb is also low. Polycystic kidney disease is diagnosed with ultrasound. Children born with this condition usually die within hours due to breathing problems. If they survive they will have kidney failure and liver problems.

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what is the role of the electron transport chain in forming the h gradient across the inner mitochondrial membrane?

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The energy released as electrons are passed down the electron transport chain is used to pump H+ from the mitochondrial matrix to the intermembrane space creating an H+ concentration gradient.

The transfer of electrons from electron donors to electron acceptors via redox reactions is accomplished by a group of protein complexes and other molecules that connect protons (H+ ions) through a membrane.

It is an exergonic method of transporting electrons. Due to an electrochemical proton gradient produced by the redox reaction energy, adenosine triphosphate produced (ATP). Through the exergonic flow of electrons from NADH to FADH2, the chain serves as an energy converter that pumps H+ from the mitochondrial matrix to the intermembrane gap. The gradient of the H+ is likely to disperse on its own when it returns across the membrane. And the ATP synthases are the only areas of the membrane that let H+ enter through.

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globally, primary production in terrestrial ecosystems tends to group of answer choices increase with increasing secondary production be most limited by the amount of nitrates in the soil increase with decreasing temperature increase with increasing rainfall

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Both the predominance of soil over water at the surface and the expansion of plants above this soil/water surface distinguish terrestrial ecosystems from aquatic ecosystems.

A population of land-based organisms and the interactions between biotic and abiotic components in a particular area make up a terrestrial ecosystem. Terrestrial ecosystems have a wide range of water availability (including water shortage in some circumstances), but in aquatic ecosystems, water is rarely a limiting factor for species. Terrestrial ecosystems often endure higher diurnal and seasonal temperature changes than aquatic ecosystems in similar regions because water cushions temperature swings. In order to achieve Sustainable Development Goal, which focuses on the conservation, restoration, and sustainable use of terrestrial ecosystems, terrestrial ecosystems are especially crucial.

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describe two different ways that a cell’s shape can be modified so that diffusion rate will be increased to support life processes. give an example of a cell found in the human body that meets one of these criteria. explain how that unique shape is tied to the function that those cells perform.

Answers

The cell can be modified in the following ways to speed up the diffusion of materials into and out of it:

1. The cell's shape can be altered to one with a large surface area to volume ratio, such as a sphere.

2. To improve the surface area to volume ratio, the cell can also divide into two.

What is diffusion?

In bacteria, plants, and animals, the diffusion of tiny molecules and macromolecules (such proteins) in aqueous solutions is crucial. As fragrance components in fruit juice, coffee, and tea diffuse from solutions during evaporations, diffusion plays a significant role in food processing as well as in the drying of liquid mixtures and solutions. In fermentation, products, waste, and occasionally enzymes diffuse out while nutrients, oxygen, and sugar diffuse to the microbes. The kidneys filter the blood of wastes such urea, creatinine, and extra fluid. Patients with malfunctioning kidneys can have waste products removed from their blood through renal dialysis.

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which part of the neuron receives nerve impulses from other neurons and conducts them toward the cell body? biology reading quiz

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Part of the neuron which receives nerve impulses from other neurons and conducts them toward the cell body is dendrites.

Nerves make up the nervous system. A bundle of nerve cells is a nerve. A neuron is a type of nerve cell that carries messages. Like sunlight's rays, dendrites and axons connect to the cell's body. Neurons transmit information. They transmit information between brain regions and the rest of the nervous system by means of chemical signals and electrical impulses.

A neuron's dendrites are extensions of the cell body. They move signals toward the cell body after receiving them from sensory receptors or other neurons.

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Sequencing of a child's genomic DNA reveals that the child is a heterozygote for a new mutation. This mutation appears in all of the cells tested from a variety of tissues (including skin, blood, and hair). The mutation is not found in either the mother or the father. Most likely, this is a new ____ mutation that occurred in O germ line; one of the parents O germ line; the child O somatic; the child Osomatic; one of the parents O germ line; both of the parents

Answers

Most likely, this is a new somatic mutation that is to occur in a child which appears in all of the cells tested from a variety of tissues.  

So, the correct option is C.

Using whole genome sequencing, you can examine and contrast the germline DNA of your child with the DNA of cancer or tumor cells. We may learn more about the specific diagnosis and course of treatment for your child from these, which can vary. Deciphering the genetic material found in an organism or virus is done by scientists using a process called genomic sequencing. According to scientists, the spread of a virus, how it is changing, and how those changes may affect public health can all be tracked by comparing sequences from specimens.

In genetic syndromes, two types of tests are used: molecular and cytogenetic. In general, methods such as restriction enzyme digestion, DNA hybridization, and/or direct sequencing are used to detect single-base pair mutations.

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Several body systems work together to regulate the pH of body fluids within a very narrow range. Click to select the problems that can occur when the pH of body fluids gets too high (alkalosis) or too low (acidosis). Reduced function of blood transport proteins Hypertension Fatigue Decreased rate of chemical reactions Dizziness Fishy body odor

Answers

Fatigue and lightheadedness In experimental animal models, acidosis has been found to activate vasopressin, adrenocorticotropic hormone, and aldosterone.

As a result, the blood pressure may rise; however, hypotension may also happen. An severe amount of fatigue as well as nausea and vomiting are signs of metabolic acidosis..

Because of their incredible biological design, our bodies can fend off harmful microorganisms like bacteria, viruses, infections, and disease.

The immune system of the body is in charge of eliminating these foreign substances and fortifying defenses against additional damage.

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How many times does a restriction enzyme cut a double stranded piece of dna at a given restriction site?.

Answers

A restriction enzyme is able to cut a segment of DNA several times. The number of times the DNA is cut (4n) depends on the length of the recognition site (n).

A restriction enzyme cuts how many times?

According to statistics, an enzyme makes an average of one cut per 4n base pairs, where n is the length of the recognition site. As a result, a DNA molecule's likelihood of containing one or more restriction sites increases with length.

Where on the double-stranded DNA are restriction enzymes active?

Restriction enzymes (RE) are endonucleases that recognize particular DNA sequences between four and eight base pairs in length and normally break the strands at a specified and constant point within or before the recognition site.

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angiosperms consist of the clades a) cotyledons and roots. b) gymnosperms and angiosperms. c) cycads and mosses. d) monocots and eudicots.

Answers

Angiosperms consist of the clades of monocots and eudicots. Therefore, the correct answer is option D.

Angiosperm is the term that encompasses most plants that bear flowers and fruits (flowering plants). It consisted mainly of two clades: monocots and eudicots.

Monocots are grass and grass-like flowering plants. There are more than 60 thousand species in this clade. Some examples of monocot plants are wheat, palm, corn, and bamboo.

Eudicots are flowering plants that generally have two seed leaves upon germination. There are roughly 190,000 species in this clade. Some examples of eudicots are dandelion, sunflower, rose, mango, apple, and maple.

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what are homologous features? explain why they are important in biological classification, and give an example of a homologous feature.

Answers

Answer: Homologous structures are organs or skeletal elements of animals and organisms that, by virtue of their similarity, suggest their connection to a common ancestor. These structures do not have to look exactly the same, or have the same function. The most important part, as hinted by their name, is that they are structurally similar.

Explanation:

Sudoriferous glands....
a. sweat
b. sebum
c. milk
d. cerumen

Answers

The answer is
A. Sweat

What are some of the positive things that happened in America as a result of WWI? What new
opportunities
did people have?

Answers

Women’s rights was something positive born out of WWI, women were needed to do more than stay at home and were asked to apply to factory jobs due to the boom in weapons, loss in men due to the enlistment, and the economy being in need of support.

which of the following is/are involved pathophysiology of als? group of answer choices death of upper motor neurons death of lower motor neurons loss of dopamine neurons in the substantia nigra

Answers

Pathophysiology. The pathogenesis of ALS includes motor neuron loss in the majority of brainstem motor nuclei, the motor cortex, and the spinal ventral horns.

What purpose do brain nuclei serve?

The neuronal networks that regulate somatic movement and motivation are thought to be significantly influenced by the cerebral nuclei, which make up the ventral division of the cerebral hemisphere.

Which somatic movement is an illustration?

progressively relaxing the muscles By clenching and releasing each muscle group, you are moving your muscles just slightly to release tension in your body and mind. This popular relaxation practice is actually a perfect illustration of somatics.

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what infectious agent produces cysts that transmit the condition via contaminated food and water and have the ability to survive for long periods in cold water? group of answer choices salmonella typhi escherichia coli vibrio cholera giardia lamblia

Answers

Giardiasis and cryptosporidiosis are transmitted by sick people or animals whose faeces contain invasive cysts and oocysts.  

Giardia and Cryptosporidium are spread through faeces and oral fluids. Swimmers in open pools might become infected by ingesting water or drinking it.Giardia cysts can contaminate surfaces, food, and water, and when consumed during this contagious stage of their life cycle, they can result in giardiasis. When a person ingests Giardia cysts from contaminated water, food, hands, surfaces, or objects, they get infected. The importance of the four main pathogen groups—helminths, protozoa, bacteria, and viruses—is summarised before the significance of waterborne disease is discussed in relation to the overall world disease burden.

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describe the locations for the peripheral ganglia for the sympathetic and parasympathetic nervous systems.

Answers

There are sorts of autonomic ganglia: the sympathetic and the parasympathetic primarily based totally on their functions. The former have a tendency to be located near the spinal wire while the later lie close to or inside the viscera of the peripheral organs that they innervate.

The top thoracic sympathetic ganglia are located across the heads of the ribs and are included through the pleura. The decrease or 3 ganglia are on the perimeters of the vertebral bodies. The thoracic sympathetic trunk runs among the ganglia and simply in the front of the somatic nerves.

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