Conjugated enzymes are a key component of ELISA. To what are these enzymes conjugated, and why is that important?

Answers

Answer 1

Answer:

To the antigen so the enzymes are only present when the molecule of interest is also present

To the plastic of the reaction well so the enzymes can concentrate the molecule of interest

To the capture antibody so the enzymes are only present when the molecule of interest is also present

To the detection antibody so the enzymes are only present when the molecule of interest is also present

Explanation:


Related Questions

Uncellular eukaryotic organisms belong to?

Answers

All eukaryotic unicellular organisms belong to Protista.

Answer:

Unicellular eukaryotic organisms belong to kingdom protista.

Explanation:

Unicellular eukaryotic organisms belong to kingdom protista. They depend on other organisms for their food since they're not able to produce their own.

Hope this helps :)

What was the population density of the original sample? What would have happened if you had inoculated an agar plate with 1 mL of the original sample?

Answers

Answer:

The sample was at a population density of 8.75x10^4 CFU/mL originally.

Explanation:

Had it been inoculated in the way as described in the question statement, the bacterial growth would have covered all of the agar plate and we would not have been able to distinguish individual colonies of bacteria. 1 mL is too much of a sample volume to use in cases like these.

As Hite and Seitz (2016) discuss in Chapter 5: Climate Change, reputable scientific studies continue to show that the Earth is warming at an unsustainable rate because of a rise in the release of CO2 and methane gases. What is the relationship between population increases and climate change

Answers

Answer:

See the answer below

Explanation:

The relationship between climate change and increased population of humans is a direct one. As the population of humans increases, their activities leading to the release of greenhouse gases such as CO2 and methane also increases. The greenhouse gases are primarily responsible for the warming of the earth.

As more and more greenhouse gases are released into the atmosphere, more radiation from the sun is trapped in the atmosphere and the earth receives more warmth. This extra heat received leads to increase in the global temperature, a phenomenon known as global warming. As the average global temperature increases, climate becomes more erratic and unpredictable.

The greenhouse gases-related activities of humans that are bound to increase with the increase in population include deforestation, fossil fuel burning, industrialization, etc.

the clean water act ___
set maximum contaminant levels for drinking water
required industries to reduce or eliminate point source pollution in surface waters
established national ambient air quality standards
requires that pollution control devices be installed on cars and trucks and at factories

Answers

Answer: The Clean Water Act required industries to reduce or eliminate point source pollution in surface waters

If the scientist had collected 20 dung samples, would you expect more bands, fewer bands, or the same number of bands on the gel?

Answers

Answer:

The expectations of the bands will be more on the gels.

Explanation:

One sample provides several sizes and thickness bands in the gel electrophoresis according to the size of fragments that run in it. An individual would expect more bands due to the increase in the number of samples which is 20 samples now.

An increase in the number of samples produces more bands. So one can easily expect more bands from the 20 samples. Scientists would get more bands on the gels.

Thus, the expectations of the bands will be more on the gels.

If the scientist collects 20 dung samples ; we should expect more band on the gel

Given that each dung sample has varying sizes and varying thickness of bands in the gel electrophoresis which depends on the size of fragments contained in it. Therefore when a number of dung samples ( 20 ) are collected  it is only logical that the number of bands on the gel will increase ( i.e. you should expect more band on the gel ).

Hence we can conclude that If the scientist collects 20 dung samples we should expect more band on the gel .

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Although your question lacks some data a general answer within the scope of your question is provided

A circular double-stranded DNA molecule contains 4200 base pairs. In solution, DNA is in a B-form helix, with about 10.5 base pairs/turn. The DNA has 12 superhelical turns. What is its superhelix density

Answers

Answer:

The correct answer is - 3 × 10⁻²

Explanation:

first find LKo = length of BDNA/ 10.5 bp/turn

= 4200/10.5

= 400

now calculate Δ LK= LK - LKo

ΔLK = 412-400

ΔLK = 12

now, superhelix density  = ΔLK/LKo

⇒ 12/400 = 0.03

= 3×10⁻²

Thus, the correct answer is - 3 × 10⁻²

What best describes the behavior of nonconservative elements in seawater?

Answers

The given question is incomplete due to missing options, however, the options for the question as follows:

A. Nonconservative elements are reactive in seawater and have a long residence time.

B. Nonconservative elements are reactive in seawater and have a short residence time.

C. Nonconservative elements are non-reactive in seawater and have a short residence time.

D. Nonconservative elements are non-reactive in seawater and have a long residence time.

Answer:

The correct answer is option C.

Explanation:

Non conservative components are components that enter the ocean water and have little concentration in the sea and show spatial variations. These components have short home time that is short replacements time and they are non responsive.

Constituents, for example, phosphate, nitrate, and different supplements, and broke up oxygen, carbon dioxide, and so on are non-conservative on the grounds that their concentrations are later altered by chemical reactions in the ocean.

Thus, The correct answer is option C.

Jacob wants to learn how he can help ensure that the biodiversity of earth no longer decreases. which topic should he research?

A. reducing pollution and preserving wildlife habitat.

B. areas on earth with large amounts of un-mined coal.

C. demand of rainforest timber and exotic animal's

D. energy generated from wild and high-cost windmills​

Answers

Answer:

C. demand of rainforest timber and exotic animal's

Explanation:

Biodiversity refers to the combination of biological and diversity. In this, it shows the varieties of life than could be found on earth in terms of plants, animals, etc

For not reducing the biodiversity on earth, Jacob should be researched  on rainforest timber demand and the exotic demand so that the biodiversity could not decline

Therefore the option c is correct

Hence, all the other options are wrong.

Which structure of a paramecium is analogous to a “reserve copy” of all the cell’s genes

Answers

Answer:

micro nucleus

Explanation:

How is the top layer of the ocean different than the deepest layer
A the top layer is colder and doesn’t receive sunlight
B the top layer receives sunlight and is warmer
C the top layer is warmer and doesn’t receive sunlight
D the top layer is colder and receives sunlight

Answers

Answer:

B the top layer receives sunlight and is warmer

Explanation:

Answer:

B. the top layer receives sunlight and is warmer.

which type of research study compares disease rates among population groups?

Answers

Answer:

Epidemiological

Explanation:

Epidemiology compares disease rates among population groups, and attempts to identify related conditions or behaviors.

Answer: Epidemiological studies

A microbiologist would like to use a noncompetent genus of streptococcal bacteria, Enterococcus faecalis, as a cloning host to express genes from Streptococcus pneumoniae, which is naturally competent. Is this possible? Group of answer choices

Answers

Answer:

Yes, with the assistance of electroporation the bacteria Enterococcus can be made competent, and afterward, the Streptococcus bacterial genes can be presented within the Enterococcus with the help of a cloning vector.  

The electropermeabilization or electroporation refers to a microbiology technique in which the permeability of the cell membrane is enhanced with the application of the electric field. This permits drugs, chemicals, or DNA to get presented within the cell.  

The procedure of electroporation is generally used in the field of microbiology to transform yeast, bacteria, or protoplasts of plants by presenting novel coding DNA.  

1.

behavior is inherited and



2. When a deer hears a sound in the forest, such a

then bolts through the bushes. This is an examp

3. Mating rituals or displays can be very complex

4. When animals fight and one wins and one subm

This means that one animal (the winn

behavior is used to intimidate other an

baring teeth or growling.

Answers

Answer:

Behavior is inherited in many animals which helps them to survive.

Explanation:

When animals fight with one another and one wins. This means that one animal (the winner) behavior is used to intimidate other. Two deer fight each other in order to show dominance over each other and to impress the female deer. The winner deer will be the leader and also mate with the females of the group. This is a type of behavior which is inherited in animals through genetics is called instincts.

The sex of the person that you are attracted to would
determine your
sexual identification.
o gender-role attractions
sexual orientation.
sexuality​

Answers

Answer:

Gender role attraction determine your sexual identificaton.

Explanation:

List the functions of proteins

Answers

Answer:

Growth and Maintenance.

Causes Biochemical Reactions.

Acts as a Messenger.

Provides Structure.

Maintains Proper pH.

Balances Fluids.

Bolsters Immune Health.

Transports and Stores Nutrients.

The mechanism by which proteins synthesized in the cytosol are imported into the mitochondrial matrix is different from the mechanism by which proteins enter the nucleus, yet the two mechanisms do share some features. Indicate whether each of the following statements applies to nuclear import, mitochondrial import, both, or neither. Sort each statement into the appropriate bin.
1. The polypeptide to be transported into the organelle has a specific short stretch of amino acids that targets the polypeptide to the organelle.
2. The imported protein enters the organelle through some sort of protein pore
3. The pore complex consists of more than two dozen proteins and is large enough to be readily seen with the electron microscope.
4. ATP hydrolysis is known to be required for the translocation process
5. The signal sequence is always at the olypeptide's N-terminus and is cut off by a peptidase within the organelle
6. The signal sequence is recognized
GTP hydrolysis is known to be required for the translocation process
7. There is strong evidence for the involvement of chaperone proteins polypeptide's N-terminus and isctand bound by a receptor protein inrequired for the translocation during translocation of the protein the organelle's outer membrane process.
Nuclear import Mitochondrial import Both Neither

Answers

Answer:

Nuclear import:

1. GTP hydrolysis is known to be required for the translocation process

2. The pore complex consists of more than two dozen proteins and is large enough to be readily seen through electron microscopy

Mitochondrial import:

1. ATP hydrolysis is known to be required for the translocation process

2. The signal sequence is always at the polypeptide's N-terminus and is cut off by a peptidase inside the organelle

3. There is strong evidence for the involvement of chaperones during the translocation process

4. The signal sequence is recognized and bound by a receptor protein in the organelles outer membrane

Both:

1. The polypeptide to be transferred into the organelle has a specific short stretch of amino acids that targets the polypeptide to the organelle

2. The imported protein enters the organelle through some sort of protein pore

Explanation:

what is a part of interphase A. cytokinesis B. telophase C. anaphase D. s phase

Answers

Answer:

D

Explanation:

I did it on my own soci know it's the answer

In the context of interphase, the S phase is the specific part where DNA replication occurs, contributing to the overall process of cell division and ensuring the transmission of genetic material to new cells and the correct option is option D.

Interphase is the longest phase of the cell cycle, and it is divided into three main stages: G1 phase, S phase, and G2 phase. Among these stages, the S phase is specifically responsible for DNA replication. It is during the S phase that the cell's DNA is duplicated in order to prepare for cell division.

During the S phase, the DNA helix unwinds, and the two strands separate. Enzymes and proteins then work together to synthesize new complementary strands for each of the separated DNA strands. As a result, two identical copies of the DNA molecule are produced.

The S phase plays a crucial role in maintaining the genetic integrity of the cell. By accurately duplicating the DNA, the cell ensures that each daughter cell formed during cell division receives an identical set of genetic information. This is vital for the proper functioning and development of organisms.

Thus, the ideal selection is option D.

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A friend comes to you extremely agitated and says her husband beat her last night.She says she doesn't know what to do.You Can See She's Very panicky.Everything you suggest just seems to provoke more anxiety and fears.When you try to respond to one aspect of the problem.Your friend interrupt's frantically and jumps to another.Explain what can you do to help.

Answers

Let her get it all out and then try to help her . If she keeps panicking u might wanna take her to get some help

Jessica weighs 125 pounds. She rode a bike at 17mph for 25 minutes. What is the calorie cost of this activity?
a. 185 calories
b. 178 calories
c. 180 calories
d. 190 calories
e. 188 calories

Answers

Answer:

b. 178 calories

ok so 17mph is 0.57 weight/min

so what were going to do is multiply 125 times 0.57 which is 7.125

then multiple that answer by 25 mintues: 7.125 x 25 = 178.12...etc

now if we round this up it stays as 178!

which describes a meta phase plate

Answers

Answer:Metaphase plate refers to sister chromatids lining up in the center of the cell. This event occurs during the metaphase of the cell division and it is the indication of this phase. Metaphase plate is a moment when all of the chromosomes organize, line up literally in a row and after that begin to.

 What is the most likely explanation for how speciation occurs?
A. It occurs by more than one method.
B. It occurs by gradual changes.
C. It occurs only by geographic isolation.
D. It occurs only by natural selection.

Answers

Answer:

The answer is (C) geographical isolation

Explanation:

Speciation is most likely to occur when populations become geographically isolated from one another, and come to inhabit environments that differ significantly from one another.

If there are 12 chromosomes in an animal cell in the G 1 stage of the cell cycle, what is the diploid number of chromosomes for this organism?

Answers

Answer:

The gametes are haploid, having half the number of cells as compared to somatic cells. The gametes or sex cells produced by the organism will have 6 chromosomes if the organism has 12 chromosomes

Explanation:

A chemical reaction is an interaction that always causes:
O A. matter to be changed into energy.
B. macromolecules to be formed from glucose.
O C. molecules to break apart into atoms.
O D. atoms to be rearranged into new substances.

Answers

Answer:

D. atoms to be rearranged into new substances.

Explanation:

Chemical reactions involve interaction between chemicals such that all reactants are changed into new materials. The properties of the new materials are different from those of the reactants.

Chemical reactions involve breaking chemical bonds between reactant molecules (particles) and forming new bonds between atoms in product particles (molecules). The number of atoms before and after the chemical change is the same but the number of molecules will change.

Which activity involves a chemical change?
A. Ice melting into liquid water
B. A metal rod being bent into a ring
C. Colorless egg becoming a white solid
D. Salt dissolving in water
SUBMIT
Need help now pls!!

Answers

option C is correct........

.. Please follow me and mark as brainliest

Humans breathe in oxygen and release ____ gas.

Answers

Answer:

Carbon

Explanation:

Reverse photosynthesis

Answer:

carbon dioxide

Explanation:

our body changes it

why was an Archea considered as a prokaryote ​

Answers

Answer:

Archaea is prokaryotic because it doesn't have a nucleus, nor any membrane-bound organelles. The reason why Archaea is different from Bacteria is it is more prominent, most of them are extremophiles.

because they are composed of cell which doesn't have nucleus and other some cell organelles and also considered as incomplte cell.

Huntington's disease, which causes degeneration of neurons, runs in Mark's family. Huntington's is a heritable autosomal dominant genetic disease, in an autosomal dominant disease, only one abnormal allele is required to cause the disease in offspring. However, it is possible not all offspring will receive the abnormal allele from an affected heterozygous parent.
Family members that have the disease include Mark's maternal grandfather and Mark's maternal aunts. Mark's maternal grandmother does not have the disease.
Because symptoms of Huntington's disease do not appear until later in life, genetic testing is necessary to determine whether a younger individual possesses the allele for the disease. These tests determined that Mark's mother does not carry the allele for Huntington's disease.
Mark's paternal aunt suggested that Mark create a pedigree to track the disease in his family. Use the symbols provided to complete the pedigree. Each symbol may be used more than once.

Answers

Answer:

As it is given that Mark's paternal grandmother and grandfather does not carry the disease. The symbols on the right side of the Punnett represent Mark's paternal family. If both his paternal grandparents are unaffected then his father and paternal aunt are also not affected by the disease.

His maternal grandfather is affected as mentioned and also his maternal aunts are affected. Mark's mother is unaffected as she does not have the disease-causing alleles determined by genetic testing.

Both of the marks parents are unaffected which indicates that marks also do not carry the allele of the disease.

Huntington's disease is an autosomal dominant disorder in which the presence of even one dominant allele can cause the disorder.

The autosomal disorder can not skip a generation, have to be present in every generation.

Condition Given:

Affected Member: Mark's Maternal grandfather and Maternal aunts.

Normal: Mark's Maternal grandmother and Mark's Mother.

As MArk's Maternal Grandfather is affected then his genotype must be Aa because Mark's Maternal grandmother is unaffected (aa), this is the only condition where Mark's mother will be normal.

A- Dominant (Huntington)

a- Recessive

Maternal means on the mother's side and paternal means on the father's side.

The diagram is attached below.

I-1: Mark's maternal Grandmother

I-2: Mark's maternal Grandfather

I-3&4: Mark's Paternal Grandparents

II-1&2: Mark's Maternal aunts

II-5: Mark's paternal aunt

II-3&4: Mark's mother and father respectively will not have the disease as both father and mother of Mark are normal.

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The graph below shows the temperature of ocean water at different depths. Which part of the graph represents the thermocline? a. AB b. BC c. CD d. DE

Answers

Answer:

C

Explanation:

Generally temperature changes rapidly with the dept in aquatic bodies especially the oceans.The thin distinct or transition layer where the variation in temperature with depth is most significant is called Thermocline.

Its represents the gaps which  separate   the upper mixed layer from the lower deeper water bed below,it therefore represent the layer of mixture of the upper surface warmer water (C) below 200 meters, with the  cooler water layer(E) below,from 1000 meters check the attachment.

It is caused by variation in intensity of sunlight.The latter hits the surface water layer and therefore raises the temperature.But,because of decrease in  sunlight penetration,with  increasing  dept, the temperature of the  lower water dept decreases.

difference between plant and animal cyanide resistance respiration.

Answers

Answer:

The cyanide resistant respiration differs in plants as oxi­dation of reduced coenzyme continues even in the presence of cyanides.

Explanation:

The mitochondrial is the electrons through which the animals and plants perform the aerobic exercise and is blocked by the release of the cyanides that stops the activity and is known as cyanide sensitive respiration Plant mitochondria is different from the animal as they both have different oxidase system pathways. Cyanide resistant is responsible for the climacteric in fruits and for the generate heat in thermogenic tissues.

Which type of mutation occurs only in gametes?
germline mutation

somatic mutation

point mutation

frameshift mutation

Answers

Answer:

Germline mutation

Explanation:

A germline mutation is a genetic change produced in the gametic-germline cells (i.e., male and female gametocytes). The mutations in the germline are inherited in the next generation, while changes in somatic cells are not inherited. Moreover, the germline mutations may occur either before the fertilization or during embryonic development (zygote morula, blastula and gastrula). This type of mutation may be induced by both endogenous (e.g., oxidative DNA damage) and exogenous (e.g., exposure to UV light) factors.

The type of mutation that occurs in gametes only is : ( A ) Germline mutation

Germline mutation is a type of mutation that occurs in the gametocytes ( male and female ) and is passed( inherited ) on to the offspring ( next generation ). while mutations occurring in somatic cells are not passed on to the next generation .

Germline mutation is induced by either endogenous factors or exogenous factors and this is because Germline mutations can occur before fertilization or after fertilization.

Hence we can conclude that the type of mutation that occurs in gametes only is Germline mutation.

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As Danny was pouring cereal for his breakfast, he noticed that the cereal box says that the cereal contains 5 milligrams of iron per serving. Danny decided to perform an experiment to test this claim. He found that the cereal contains 4.6 milligrams of iron per serving. Based on Danny's measurement, the value reported on the label is ( CORRECT OR INCCORECT) because A: (IT IS DIFFERENT FROM THE VALUE DANNY MEASURED) B: (IT IS REORTED TO THE NEAREST MILLIGRAM) C: (IT USES THE SAME UNITS THAT DANNY USED). Which statement best identifies the main point of chapter 9 in the fast food nation ? In the United States banking policies and procedures are set by the:-president -senate -House of representatives-federal reserve A newsgroup is interested in constructing a 90% confidence interval for the proportion of all Americans who are in favor of a new Green initiative. Of the 559 randomly selected Americans surveyed, 370 were in favor of the initiative. Round answers to 4 decimal places where possible.a. With 90% confidence the proportion of all Americans who favor the new Green initiative is between and .b. If many groups of 506 randomly selected Americans were surveyed, then a different confidence interval would be produced from each group. About percent of these confidence intervals will contain the true population proportion of Americans who favor the Green initiative and about percent will not contain the true population proportion. Erbium metal (Er) can be prepared by reacting erbium(III) fluoride with magnesium; the other product is magnesium fluoride. Write and balance the equation. If 40% of the total population of a particular town of 2500 are HIV positive. How many people are HIV negative. In order to clear out room for new merchandise, James decided to mark down some of the items for sale in his electronics store. He marked down DVD players by 36%, and he marked down stereo tuners by 22%. If DVD players cost $41.60 after the markdown and stereo tuners cost $69.42 after the markdown, which items price was reduced by more, and by how many dollars more was it reduced? Round all dollar values to the nearest cent. You have an opportunity to invest in Australia at an interest rate of 8%. Moreover, you expect the Australian dollar (A$) to appreciate by 2%. Your effective return from this investment is Verb Tenses A: My goodness! What is that strange noise? B: Oh, nothing. Thats just my roommate. He always (sing) ____ in the shower. a. singing b. sung c. is sing d. sings The equations 2 x minus y = negative 2, 3 x + 2 y = 5, 4 x minus y = 2, and 22 x + 10 y = 7 are shown on the graph below. On a coordinate plane, there are 4 lines. Green line goes through (0, 2.5) and (1.75, 0). Blue line goes through (0.5, 0) and (1, 2). Pink line goes through (negative 1, 0), and (0, 2). Purple line goes through (negative 0.75, 2.5) and (0, 0.75). Which system of equations has a solution of approximately (0.3, 1.4)? 2 x minus y = negative 2 and 22 x + 10 y = 7 3 x + 2 y = 5 and 4 x minus y = 2 4 x minus y = 2 and 22 x + 10 y = 7 2 x minus y = negative 2 and 3 x + 2 y = 5 Who wrote the new Japanese constitution?British officialsmembers of thy Dietthe Allied governmentsUS Army officers What happens to the entire respiratory system if one part fails to carry its function Please Help Fast! Compare and Contrast Content Follow the directions to complete the graphic organizer. Then answer the question. When you have finished, submit this assignment to be graded. In the circle on the left, describe content and characteristics that make the 1973 Nixon Press Conference Video & Transcript different from the other text. In the circle on the right, describe content and characteristics that President Richard M. Nixon: Re-election, Second Term, and Watergate different from the video and the transcript. In the area where the circles overlap, describe the ways in which the pieces are alike. 1973 Nixon Press Conference President Richard M. 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These two species of Amblyrhynchus agree, as I have already stated, in their general structure, and in many of their habits. Neither have that rapid movement so characteristic of the genera Lacerta and Iguana. They are both herbivorous; although, the kind of vegetation on which they feed is so very different. Mr. Bell has given the name to the genus from the shortness of the snout; indeed, the form of the mouth may almost be compared to that of the tortoise: one is led to suppose that this is an adaptation to their herbivorous appetites. It is very interesting thus to find a well-characterized genus, having its marine and terrestrial species, belonging to so confined a portion of the world. The aquatic species is by far the most remarkable because it is the only existing lizard which lives on marine vegetable productions. As I at first observed, these islands are not so remarkable for the number of the species of reptiles; as for that of the individuals, when we remember the well-beaten paths made by the thousands of huge tortoisesthe many turtlesthe great warrens of the terrestrial Amblyrhynchusand the groups of the marine species basking on the coast-rocks of every islandwe must admit that there is no other quarter of the world where this order replaces the herbivorous mammalia in so extraordinary a manner. What differences did Darwin find about the two species? One is fast, while the other is slow. One eats plants, while the other eats meat. One lives inland, while the other lives in the water. One has a long snout, while the other has a short snout. Read the story summary.What kind of nonfiction source did the author mostlikely use to help make the elements in the storyrealistic?While visiting a meteor crater, a teenager comes acrossa meteorite that inspires him to become an astronaut.O an animated film about a group's voyage throughthe galaxyO a blog where people speculate about the existenceof aliensO a popular television series set in the far reaches ofspaceO a government website on space exploration supose at 20 degree celsius the resistance of Tungsten thermometer is 154.9. WHen placed in a particular solution , the resistance is 207.4 What is the temerature (in degree Celsius of this solution Module 7When you multiply a difference of two squares, why is your answer a binomial instead of a trinomial like when you multiply the sum of two squares? Create an example of multiplying a difference of two squares and show your work as you simplify the expression.WILL GIVE BRAINLIEST approximately 13% of the USA population is left handed. Hiw many lefties would expect in a school of 420 students The owner of a fish market has an assistant who has determined that the weights of catfish are normally distributed, with mean of 3.2 pounds and standard deviation of 0.8 pound. If a sample of 4 fish is taken. What is the probability that the sample means will be more than 3.4 pounds? HEEELPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPP