Blue-white screening is a technique for the identification of___. It relies on the ability of____to cleave lactose. Blue-white tests take advantage of a molecule called____which is similar to lactose in that it is cleaved by B-galactosidase. When cleaved, the____(same as previous) turns . If uncleaved,meaning a non- function B-gal gene, the X-gal remains____. Therefore, a____bacterial colony means the B-galactosidase gene is not-functional, and therefore there____a recombinant gene inserted into the vector.
You’ve inserted a gene for pesticide resistance into your vector. To see if it was successful, you decide to conduct a blue-white screening test. But, uh-oh you are out of X-gal! You do notice some green Slime-gal sitting on the shelf and recall that this molecule works similarly to X-gal, but instead of turning blue when cleaved, it turns green when cleaved. You carry out the test, and find that all of your colonies are green! Was your insertion of the pesticide gene successful?

Answers

Answer 1

Answer:

Explanation:

Blue-white screening is a method for distinguishing proof of (recombinant bacteria). It depends on the capacity of ( B-galactosidase) to separate lactose. Blue-white tests exploit the molecule called  (x-gel)_ which is like lactose in that it is severed by B-galactosidase. When separated, the (5-bromo-4-chloro-indoxyl) (same as past) turns _(_blue). In the event that uncleaved, which implies a non-function B-gal gene, the X-gal remains (white)_. Subsequently, a __(white) bacterial province implies the B-galactosidase gene isn't practical, and in this way there ___lacz__ a recombinant gene embedded into the vector.


Related Questions

Which of the following factors decrease biodiversity? (Choose all that apply)
a. habitat destruction
b. unpredictable precipitation
c. warm temperatures
d. freezing temperatures

Answers

Answer:

habitat destruction, unpredictable precipitation, and freeing temperatures.

Explanation:

i took the quiz

The destruction of the habitat, unpredictable precipitation, and freezing temperatures decreases biodiversity. Thus, options a, b, and d are correct.

What is biodiversity?

Biodiversity is the variety of life that sustains on earth and lives in a particular habitat. The biodiversity can be measured based on the ecosystem, species, and genetics. It comprises the ecological and evolutionary process.

The destruction of the habitat of a species can lead to a decrease in biodiversity as it affects the ecosystem and the ecological pyramid as a whole and will affect the population of the species.

The unpredictable precipitation can affect the species and the habitat as it may lead to floods that will destroy the habitat hence resulting in the loss of species.

Similarly, the decrease in temperature will affect many species as they will not be able to cope with low temperature resulting in their death.

 

Therefore, the unpredictable precipitation, freezing temperature, and destruction of habitat lead to a decrease in biodiversity.

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What are the two main ways bacteria cause illness?
A. By releasing toxins that poison your cells
B. By using your cells to make copies of themselves
C. By changing the genetic material inside your cells
D. By breaking down your cells for nutrients

Answers

Answer:

A. By releasing toxins that poison your cells

B. By using your cells to make copies of themselves

Explanation:

Within the body, bacteria can divide (multiply) and kill host cells by disrupting their normal functioning. Moreover, bacteria secrete toxins that are able to destroy host cells' metabolic machinery.  For instance, many bacteria synthesize exoenzymes and interferons that disrupt and destroy host cells. Bacterial toxins can be synthesized internally in order to develop sensitivity through antigenic properties.

Question 17 (1 point) Spindle fibers are structures that radiate from centrioles and play an important role during mitosis. Pick the answer choice that describes their function,
A: Cytoplasm surrounds the spindles and provides protection.
B: Spindle fibers attach to the nuclear envelope and moves the nucleus around the cell.
C: Spindle fibers attach to chromosomes at the centromere and move them to the center of the cell.
D: Spindle fibers protect the cell from damage during DNA replication. ​

Answers

Answer:

The answer is D

Explanation:

What is the Society of American Foresters?
A union
A business
A government agency
A professional organization

Answers

The answer would be D

which fish is exotic in among these common carp or mori or thaila?​

Answers

Answer:

common carp

Explanation:

Given that Exotic fishes are a type of fishes that being raised in an environment that is not native to them. Usually, exotic fishes are known for surviving easily in a country that is not originally their own.

Among the available options, COMMON CARP is a type of fish that is known for its exotic nature. So far, it has been introduced to other continents and countries other than their country of origin.

They easily reproduced and multiply, thereby easily adapt and populate their new environment.

Compare and contrast the autonomic and somatic nervous divisions with respect to
effectors, neurotransmitters, and output cell organization/anatomy.

Answers

Answer:

The somatic nervous system has sensory and motor pathways, whereas the autonomic nervous system only has motor pathways. The autonomic nervous system controls internal organs and glands, while the somatic nervous system controls muscles and movement.

Explanation:

When a meteor crosses the sky, the light given off is caused by
O Friction
Gravity
O Solar Winds
O Erosion

Answers

Answer:

the answer is EROSION, I Believe

Answer:

Friction

Explanation:

Take any two words and connect them to form a sentence about photosynthesis.
____________ and ___________ go together because_______________________.
Terms:
Calvin Cycle
Carbon Dioxide
Chlorophyll
Chloroplast
Glucose
Leaf
Light
Light Dependent Reaction
Light Independent Reaction
Oxygen
Photosynthesis
Pores
Product
Reactant
Root
Starch
Sun
Water

Answers

Answer:

Solution:

plant's takes carbon dioxide ,water ,sunlight,and minerals and produce glucose and excrete out oxygen:

Take any two words and connect them to form a sentence about photosynthesis.
PRODUCT and REACTANT go together because any reactants in a chemical equation will result in a product of the reactants.

Mechanical weathering is commonly the result of all of the following except _____.
wind
plant disturbances
gravity
humans
ice
animal disturbances
running water
chemical reactions

Answers

Answer:

The diagram represents the process of enlarging a rectangle using a scale factor of 3. The width of the original rectangle must be:

9 in.

11 in.

12 in.

17 in.

Explanation:

Can you curl your tongue? Tongue-curling in humans (T) is a dominant genetic trait. Derek can curl his tongue but his wife, Ashley, cannot. All nine of their children can curl their tongues. Complete the Punnett square based on the genotypes they most likely have.

Answers

Answer: Derek is TT and his wife Ashley tt. Thus children are Tt

Explanation: T Is dominant and t is recessive.

Derek can curl his tongue but his wife, Ashley, cannot. All nine of their children can curl their tongues. Because, the trait of Derek is TT and the trait of his wife Ashley tt.

Thus, the trait of children are Tt

Why Derek cannot curl his tongue?

The tongue rolling ability occurs due to the influence of a dominant allele of the gene. A person who has either one or two copies of the dominant allele will be able to twist their tongue. In the case that a person is born with two recessive alleles, they cannot twist their tongue.

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Please help me understand what I did wrong, Thanks

Answers

is the X^H is dominant then the percent is 100% however if the Y is dominant then you did it correctly

i need help and fast with bio

Answers

no question no answer

2. A certain recessive, lethal gene is carried on the X chromosome. (Lethal genes are those that result in death.) A man marries a woman who is hybrid for this gene. What sex ratio would be expected among the living children of this couple?
%Male
%Female​
Please help me im stuck!

Answers

The surviving children would be 66.7% female and 33.3% male. Of the female children, 50% would be expected to be carriers of this gene (like their mother)
| Xg | X
—————-
X | XXg | XX
—————-
Y | XgY | XY

Pretty ugly, but the best punnet square I can do without a link. The offspring with the “g” are those that carry the lethal gene. The male child that carries it will die, but since it is recessive, the female child will live but will be a carrier for this gene. I hope this helps.

15. Suppose a human blood cell containing a 0.9 percent solute concentration were put into a container of 30 percent solute solution. Would the blood cell get bigger or smaller? Why? In your explanation, you must tell me which area has the hypotonic solution (inside the blood cell or outside the blood cell), which area has the hypertonic solution (inside the blood cell or outside the blood cell), and which direction the water would be moving (into the cell or out of the cell).

Answers

Answer:

Smaller

Explanation:

The human blood cell has a smaller percentage of solute concentration than the solution it was placed into. This means that the cell is hypotonic in comparison to the solution, and the solution is hypertonic compared to the cell. When a cell is placed in a hypertonic solution it undergoes crenation, meaning it shrivels up. Water moves from the lower solute concentration to the higher solute concentration. The water inside the cell moves outside to the hypertonic solution. This causes the cell to become smaller.

explain how incomplete dominance differs from co-dominance

Answers

Answer:

Incomplete dominance is when the phenotypes of the two parents blend together to create a new phenotype for their offspring.

An example is a white flower and a red flower producing pink flowers. Codominance is when the two parent phenotypes are expressed together in the offspring

Explanation:

good luck

According to the law of conservation of energy, what can happen to the total
energy of a system?
A. The total energy must remain the same, but energy can change
forms and be transferred within the system.
B. Energy must be transferred out of a system if energy is transferred
into the system so that they total energy of the system stays the
same.
C. The total energy can decrease when energy is lost due to energy
transformation within the system.
D. The total energy can increase if energy is transferred into the
system from surroundings.
SUBMIT

Answers

Answer:

the total energy must remain the same,but energy can change forms and be transferred within the system

Come up with your own " pre-flight" check before driving a car. Describe the importance of each step

Answers

First thing you do is put on your seatbelt. You get the very most important step out of the way first. Second, start the car. Third, position all mirrors including the rear view mirror.

What pattern of inheritance is represented punnet square

Answers

Answer:

Autosomal Dominant Inheritance

Explanation:

Incomplete Dominance

The disease multiple sclerosis makes it more difficult for signals from the brain and spinal cord to travel along nerve fibers. How will multiple sclerosis
affect other body tissues?
O Connective tissue will increase its fat storage.
O Muscle tissue will contract uncontrollably.
Epithelial tissue will lose the ability to protect against infection.
Tendons and ligaments will become brittle and break.

Answers

Answer:

O Muscle tissue will contract uncontrollably.

Explanation:

Multiple sclerosis, also known as encephalomyelitis disseminata, is a disease that affects the brain and spinal cord. Some of the most common symptoms of multiple sclerosis include vision problems, arm or leg movement and balance. This disease occurs when the immune system attacks the myelin sheath that covers nerve cells in the brain and spinal cord, causing inflammation and subsequent damage of the sheath and the nerve fibers it surrounds. Multiple sclerosis may cause muscle twitches when nerve fibers are damaged, thereby disrupting the signaling between nerves and muscles.

Summer is coming and we are ready to get out and get in the sun. However, sun light has some ultraviolet light. When a UV photon is absorbed by a molecule of DNA, there are different ways the energy can be absorbed. One of the ways is that the energy of the photon can be converted into vibrational energy of the molecular bonds. Excessive vibration can damage the molecule by causing the bonds to break. UV light of less than 290 nm causes significant damage to DNA and UV light of longer wavelength causes minimal damage. What is the threshold photon energy, in eV, for DNA damage

Answers

Answer:

The threshold photon energy for DNA damage = 4.28 eV

Explanation:

According to the Planck - Einstein's equation, the energy of a photon, E is proportional to its frequency; E = hf where f is frequency and h is Planck's constant.

Also, frequency, f = velocity /wavelength

f = c/λ where c is the velocity of light

E = hc/λ

h = 6.63 × 10^-³⁴ Js;

c = 3.0 × 10⁸ m/s;

Threshold wavelength, λ = 290 nm = 2.9 × 10^-7 m

E = (6.63 × 10^-³⁴ × 3.0 × 10⁸) / 2.9 × 10^-⁷

E = 6.85 × 10^-¹⁹ J

1 ev = 1.602 × 10^-¹⁹ J

6.85 × 10^-¹⁹ J = 6.85 × 10^-¹⁹ J × 1 eV / 1.602 × 10^-¹⁹ J = 4.28 eV

Therefore, the threshold photon energy for DNA damage = 4.28 eV


PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

The answer is D, because student 2 exercises regularly

Answer:

the answer is d, student 2 exercises more often then student 1. he also has better sleep.

Explanation:

what do all scientific investigations begin with?

A: Forming a hypothesis

B: Conducting an experiment

C: Asking a question or observing a problem

D: Forming a conclusion

Answers

A: Forming a hypothesis
I would think it’s C because in order to conduct an experiment you need to have a reason or purpose to it so you would start off with a question

If several species of carnivores are removed from an ecosystem, the most likely effect on the ecosystem will be an increase in stability among populations.

Answers

i think so since carnivours could lead to effects on ecosystem such as the number or behaviour of their herbivore prey

Explain briefly why personal care professionals must study physical anatomy

Answers

Answer:

By understanding how the systems interact, you can determine the proper care for each individual patient and their specific symptoms. With a foundation of anatomy and physiology, you will have the building blocks to make the proper decisions and provide accurate and quality care

Explanation:

g Type I diabetes is caused by destruction of the beta cells of the pancreas, the cells that produce insulin. In most cases, the beta cells are destroyed by an immune response. Both genetic and environmental factors are involved in triggering the immune response. A. The immune system usually does NOT destroy the beta cells. If the cells are destroyed, it is a failure of (memory) (specificity)(tolerance) (any one of these) (none of these).

Answers

Answer:

it is a failure of tolerance (self-tolerance) and specificity (recognition)

Explanation:

Type 1 diabetes is a chronic disease caused by the selective destruction of β cells that are involved in the production of insulin in the pancreas. Type 1 diabetes can be classified into two types: Type 1A diabetes (the immune form of the disease) and Type 1B diabetes (the non-immune form of the disease). Type 1A diabetes is considered an autoimmune disorder where immune responses against pathogens suffer a failure of tolerance to antigens in the β-cells of the pancreatic islets. Thus, Type 1A diabetes is characterized by the process of recognition of β-cell antigens (autoantigens) by the immune system. This disease is often caused by genetic factors associated with mutations in the human leukocyte antigen (HLA) class II region located on chromosome 6.

If you squeeze the bottom of a closed tube of toothpaste, the pressure is increased ____.
a.
on the bottom of the tube
c.
on the sides of the tube
b.
on the top of the tube
d.
everywhere in the tube


Please select the best answer from the choices provided

A
B
C
D

Answers

D. Everywhere .............

What part do humans play in gene flow?

Answers

Answer:

Humans reproduce and pass on their genes to their offspring.

Explain the importance of using engine powered pump for irrigation works.​

Answers

Answer and Explanation:

Engine irrigation pumps are used to propel water into the irrigation channels, providing full water supply to agricultural crops. The use of the engine pump has advantages and disadvantages. Among the advantages, we can present the simplicity of operation of these pumps, being easily operated efficiently. They also save energy and are essential for irrigation with more viscous liquids, when necessary. This feature is the main reason for using this type of pump.

Among the disadvantages we can say that these pumps have a slower and less accurate work.

Moving substances from a lower concentration to a higher concentration requires _____. passive diffusion facilitated diffusion active transport pinocytosis

Answers

Answer:

Active Transport <3

Explanation:

the law of conservation of mass means that n an isolated system matter cannot be_______

Answers

Answer:

Matter cannot be created nor destoyed

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