An acid-fast staining method was performed on a bacterial smear using the dyes carbol fuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?

a. No acid-fast bacteria are present on the slide
b. Methylene blue was not used
c. Carbolfuchsin was not used
d. Acid-alcohol was not used

Answers

Answer 1

Answer: Only blue cells where present because No acid-fast bacteria are present on the slide. The correct option is A.

Explanation:

Acid fast staining method also called Ziehl-Neelsen (Zn) technique is used in the laboratory to stain samples suspected to contain Mycobacterium species. The Mycobacterium species which includes the M. tuberculosis, M. ulcerans, and M. leprae, do not stain well by the Gram technique, which is a common routine stain in the laboratory. This is because of waxy coating on its cell surface primarily due to the presence of mycolic acid.

The acid fast stain consists of:

--> A primary dye which is the carbol

fuchsin

--> A decolorizer which is the acid alcohol

--> A counter stain which is the methylene blue.

In it mechanism of action on the bacterial smear, when the primary dye is applied,the whole cell will appear red. But after decolorizing with acid alcohol, only acid fast bacteria will retain the red colour of carbol

fuchsin while the red dye from the background cells are removed. Then the counterstain (methylene blue) is applied which is picked up by non-acid-fast bacteria making the cells appear blue under the microscope.

Therefore when the bacteria smear is viewed under the microscope and only blue cells are seen, it means No acid-fast bacteria are present on the slide.


Related Questions

One of the purposes of transcription is to produce a sequence of bases that

А. transforms into carbohydrate molecules.

B. replicates molecules of DNA without using sugars.

C. codes for the manufacture of a specific protein.

D. substitutes for essential amino acids.

Answers

Answer:А. transforms into carbohydrate molecules.

Explanation:

5. How does the current leaving the cell in a parallel circuit compare
with the current in a series circuit, if both circuits have the same
type of cell and the same number of identical flashlight bulbs?

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Answer:

circuit compare

with the current in a series circuit, if both circuits have the same

type of cell and the same number of identical flashlight bulbs?

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Answer:

i dont know sorry

Explanation:

Match the elements to their periodic table groups based on their uses or properties

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do you have a picture? as I’m unsure which elements need matching

Which sign best represents the role of decomposers?

Answers

Decomposers eat the dead/decaying life, and eventually give it back to the earth. So recycling (B) seems like the best answer.

According to the context, recycling sign is the one that best represents the role of decomposers.

What is the role of decomposers?

They are those who take care of taking advantage of the matter and energy that the remains of animals and plants present.

Thanks to them it is possible to recycle various elements by decomposing the organic matter of said organisms, until transforming them into inorganic matter.

Therefore, we can conclude that according to the context, recycling sign is the one that best represents the role of decomposers.

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A desert has a lower species richness than a tropical rainforest.
O True
O False

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Answer:

True

Explanation:

which three sentences describe ways that droughts harm savanna ecosystems?

Answers

Answer:

b a d

Explanation:

Answer:

A. , C. , D.

Explanation:

Ap3x

State the function of centromere​

Answers

Answer:

The primary function of the centromere is to provide the foundation for assembly of the kinetochore, which is a protein complex essential to proper chromosomal segregation during mitosis. In electron micrographs of mitotic chromosomes, kinetochores appear as platelike structures composed of several layers

Innocent suspects will never falsely confess to crimes they did not commit.
True
False

Answers

Answer: true

Explanation: I think it’s true. I hope I could help

what is an example of a transform boundary? I WILL GIVE YOU THE BRAINLIEST!!!!​

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San Andreas Fault Zone of western North America. The San Andreas connects a divergent boundary in the Gulf of California with the Cascadia subduction zone.

why is cancer considered a genetic disease of somatic cell?​

Answers

The transformation of a normal cell into a cancer cell takes place through a sequence of a small number of discrete genetic events, somatic mutations: thus, cancer can be regarded properly as a genetic disease of somatic cells

Thermococcus and thermoplasma what do they have in common?

Answers

Explanation:

Both Thermococcus and Thermoplasma are unicellular organisms that can live in extreme environments. Based on the concept of domains, what do they have in common? They both belong to domain Archaea. ... They both belong to domain Eukarya.

Helppp me pls :$$)))

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Answer:

A. containers A and B represent mixtures

What part of the brain is highlighted in the diagram below?

The highlighted portion is at the rear base of the brain, behind the brain stem.

Pituitary gland
Cerebrum
Cerebellum
Cerebral cortex

Answers

C. Cerebellum is your answer

The part of the brain which is highlighted with green color is known as the cerebellum.

What are the functions of the brain?

The major functions of the brain include concentration, balance, imagination, mental flexibility, coordination, etc.

The cerebellum part of the brain is located below the cerebral cortex besides the medulla oblongata. The major function of this part of the brain includes balance maintenance and posture adjustment.

Therefore, the part of the brain which is highlighted with green color is known as the cerebellum.

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Which sensory receptor is found in the skin?

Answers

Answer:

The cutaneous receptors' are the types of sensory receptor found in the dermis or epidermis. They are a part of the somatosensory system. Cutaneous receptors include cutaneous mechanoreceptors, nociceptors (pain) and thermoreceptors (temperature).

Explanation:

I think it will be heplful

Pls answer

The human body needs minerals to survive. Without
minerals, the body could not function properly. The minerals
that make up rocks are the same minerals that someone
might eat in a bowl of cereal! The body needs about 80
different types of minerals to function properly. Iron enriches
the blood, salt maintains fluid balance, calcium fortifies the
bones, and fluorine strengthens the teeth. Most vital
minerals can be absorbed into the body from eating plants
and lean meats. However, many food manufacturers add
minerals to their products to make them more desirable to
consumers. Eating a balanced diet can ensure that the
proper amount of these essential minerals is ingested.

Question:

The passage tells us that "many food manufacturers add minerals to their products to make them more desirable to consumers." Do you think that this is a good idea? What are the pros and cons?

Answers

Answer:

You have to put down what you think and not what other people thing

Explanation:

You can simply answer this question yourself by doing research or just thinking if its a good idea

Eating vegetables and lean meats can help the body absorb the majority of essential nutrients. However, many food producers add minerals to their products to increase customer appeal.

What are nutrients?

Chemical elements known as nutrients are present in all living things on Earth. They are essential to the survival of all living things, including humans, animals, and plants.

The breakdown of food by nutrients provides energy for living things. They are utilised in every bodily function of an organism.

The majority of vital nutrients can be more easily absorbed by the body when veggies and lean meats are consumed.

To boost consumer appeal, several food companies do, however, add minerals to their products.

Although processing has many advantages, it can also be harmful and reduce the nutritional value of food.

For instance, blanching causes vitamin and mineral leaching losses. Additionally, during processing, milling and extrusion might result in the physical removal of minerals.

Thus, these are some pros and cons of the given scenario.

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O
Facial expressions - How you look in front of your audience based
on your face can affect how they perceive you. If you are nervous
and cannot control it, your fear often shows on your face. If you
smile at them while you talk, they will think that you are happy
talking to them.​

Answers

so what's the question here??

To help your investigation, here is some more data about the farm.
The chicken population was started with chicks from a different farm.
Chickens are raised in barns where bacteria can spread easily between chickens.
CJ, E. Coli, and Salmonella bacteria are all present in the barns.
Some of the E. Coli and Salmonella bacteria have alleles that make them resistant to the FQ antibiotic.
To stop disease from growing in the barn, the FQ antibiotic is fed to the chickens a lot.
Choose which way of evolution caused the FQ antibiotic resistance.
Gene Flow
Genetic Drift
Natural Selection
What is the best fit option here? Form a hyptothesis
This is my hypothesis, is it right ?
If the chicken population is consistently given the FQ antibiotic, then natural selection leads to antibiotic resistance, because some chickens could have some of the bacterias that develop mutations allele and they can resist against FQ antibiotics.Bacteria which are resistant will continue to increases and grows in number over time. In the end, the chicken population are mainly resistant bacterial, thus making this the likely mechanism of evolution.

Answers

Answer:

1) Natural selection

2) Hypothesis: The excessive use of FQ antibiotics on chickens leads to the fixation of new genes -by natural selection- that result from mutations in the bacterial genetic material, which makes these cells become even more resistant to the drug.  

Explanation:

1) Natural selection selects beneficial alleles and increases their frequency in the population.

Natural selection results in adaptation, which means the increase of the aptitude phenotype. Aptitude is the contribution of each genotype to the next generation.

In many cases adaptations, resulting from natural selection can be correlated to environmental factors or selective pressures applied by other organisms or habitat.  

Let us remember that, a mutation is a change or alteration in DNI sequences that introduce new variants. Many of these are eliminated, but some of them might succeed and be incorporated into each individual. These mutations are the ones that have been selected by natural selection.

So, in the exposed chickens´ example, we are focussing on bacteria populations.

The selective pressure or modeling environmental factor is the FQ antibiotic. The bacteria´s response to antibiotics is the survival of only those that carry mutations. Natural selection benefits these mutations. Bacteria survive and become more resistant

2) Hypothesis.  

First, remember that the hypothesis is a conjecture.   The researcher hypothesizes in order to predict what is going on or what is expected to occur. A hypothesis is a claim of how it works a relation between two or more variables. Usually, it is written in the present time.

So in this example, we want to correlate the use of antibiotics with bacteria resistance.

I would hypothezise as following,

The excessive use of FQ antibiotics on chickens leads to the fixation of new genes -by natural selection- that result from mutations in the bacterial genetic material, which makes these cells become even more resistant to the drug.

This hypothesis probably states the same as yours but turns to be more concise. The hypothesis needs to go to the point. If you want to be more specific, you can probably add some specific hypotheses to dig into your work.

 

We have that the,Natural selection caused the FQ antibiotic resistance.

Natural selection

Generally , Natural resolution causes a reaction that leads to antibiotic resistance, Mutation in micro organism make them resistant to antibiotics and when they reproduce similarly ,they will pass by this resistant trait to their offspring, which will outcomes into a completely resistant generation.

In the give up ,result is a populace of in most cases resistant bacteria.

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The largest population an environment can support is its
A. symbiosis.
B.carrying capacity.
C.population.
D.limiting factor

Answers

Answer:

B. carrying capacity

Explanation

Carrying Capacity is the maximum number of individuals one species that a particular environment can support

PLSSS HELPPPP ME GUYSSSSSSSSSS ​

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Regulating body temperature

Line B in the given graph represents the reaction with an enzyme added to substrates. How did the enzyme affect the reaction?

Answers

Answer:

a. lower activation level

Explanation:

Butterflies exposed to radiation leaking from a damaged nuclear power plant in Japan have been observed to have malformed legs and Tanner and wings.For future butterflies to have structural abnormalities and gene mutations what must be present in the affected butterflies

Answers

Answer:BODY CELLS

Explanation: dont delete its true

4. Think of 5 different muscles in your body and list them. You don't have to use the scientific name. Classify these muscles as either voluntary or involuntary and identify if they are cardiac, smooth or skeletal muscle.

Please answer ASAP​

Answers

Answer:

cardiac muscles: involuntary, cardiac

triceps: voluntary

biceps:voluntary

deltoid:voluntary

quadriceps:voluntary, skeleton

What is the name of the bond that is created when two amino acids

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Answer: peptide bonds

Explanation: I think that's the answer.

CAN SOMEONE PLEASE HELP ME WITH THIS SCIENCE QUESTION

Answers

Answer:

A biomass

Explanation:

Hopefully this should be it ive learned this in 9th grade

Answer:

Biomass energy

Explanation:

Biomass energy is energy generated or produced by living or once-living organisms. The most common biomass materials used for energy are plants, such as corn and soy, above. ref: https://www.nationalgeographic.org/encyclopedia/biomass-energy/#:~:text=Biomass%20energy%20is%20energy%20generated,heat%20or%20converted%20into%20electricity.

So when they are burned they all relate to combustion. The other three are different things. tidal energy comes from waves of water, hydro energy also comes from water, wind energy is from wind

Pandas evolved longer wrists to better eat bamboo over a long period of time and this in turn increase
their possibility of survival. This is an example of:
a. Artificial Selection
b. Adaptation
c. Biodiversity
d. Homeostasis​

Answers

Answer:

B adaption

Explanation:

The scenario in which the Pandas evolve longer wrists to better eat bamboo over a long period will increase the possibility of artificial selection. The correct option is A.

What is artificial selection?

Artificial selection, also renowned as "selective breeding," is the practice of selecting for positive traits in agricultural products or animals rather than allowing the species to change and evolve naturally, as in natural selection.

The process by which organisms can choose individual lifeforms with specific phenotypic trait values for breeding is known as artificial selection. If the selected trait has additive genetic variance, it will respond to selection, i.e., the trait will evolve.

Adaptation entails dealing with both the physiological abiotic environment such as light, dark, temperature, etc. but in addition to the intricate biotic environment.

Pandas evolved longer wrists to better eat bamboo over a long period of time and this in turn increase their possibility of survival. This is an example of artificial selection.

Thus, the correct option is A.

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PLEASE HELP ME AS QUICK AS POSSIBLE

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camouflage because they are disguising themselves as something they’re not

i need help with this biology question pls and thankyou pls help

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The answer to this question is #2 it has guanine and ribose!

What is natural selection
In a paragraph
This is for 8 grade but can someone help me

Answers

Answer:

Natural selection is a pressure that causes groups of organisms to change over time. Animals inherit their genetics from their parents or ancestors, and the environment is constantly changing.

Explanation:

(self-explanatory)

Name two types of lateral pathways for the movement of sucrose from source cell into the sieve tube

Answers

Answer:

Diffusion and active transport.

Explanation:

Diffusion and active transport are the two types of lateral pathways that is responsible for the movement of sucrose from source cell into the sieve tube. Sugar molecules are moved into the sieve elements which are the phloem cells through active transport i.e. with the use of energy. The water produces turgor pressure in the sieve elements, which pushes the sugars and fluids down the phloem tubes toward the storage sites. it also moved through simple diffusion due to difference in concentration i.e. from higher to lower concentration region.

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