A research group has sequenced the cDNA and genomic DNA from a particular gene. The cDNA is derived from mRNA, so it does not contain introns. Here are the sequences. cDNA: 5'-ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAATGCCAG CGGCCAGACTATCACCCAACTCGGTTACCTACTAGTATATCCCAT ATACTAGCATATATTTTACCCATAATTTGTGTGTGGGTATACAGT ATAATCATATA-3' Genomic DNA: 5'-ATTGCATCCAGCGTATACTATCTCGGGCCCAATTAATGCCAG CGGCCAGACTATCACCCAACTCGGCCCACCCCCCAGGTTTACACA GTCATACCATACATACAAAAATCGCAGTTACTTATCCCAAAAAAA CCTAGATACCCCACATACTATTAACTCTTTCTTTCTAGGTTACCT ACTAGTATATCCCATATACTAGCATATATTTTACCCATAATTTGT GTGTGGGTATACAGTATAATCATATA-3' a. Indicate where the intron is located. b. Does the intron contain the normal consensus splice site sequences? (Underline the splice site sequences and indicate whether or not they fit the consensus sequence.)

Answers

Answer 1

The intron in the gene sequence is located between the sequences "CGGCCCACCCCCCAGGTTTACACA" and "CCTAGATACCCCACATACTATTAACTCTTTCTTTCTAGGTTACCT". It is unclear whether the intron contains normal consensus splice site sequences due to insufficient context in the provided sequences.

a. Based on the provided sequences, we can determine the location of the intron by comparing the cDNA and genomic DNA sequences.

The intron is the segment that is present in the genomic DNA but absent in the cDNA. By aligning the two sequences, we can identify the region that is unique to the genomic DNA.

In this case, the intron is located between the sequences:

CGGCCCACCCCCCAGGTTTACACA

and

CCTAGATACCCCACATACTATTAACTCTTTCTTTCTAGGTTACCT

b. Consensus splice site sequences are specific DNA motifs that mark the boundaries of introns and are recognized by the splicing machinery during mRNA processing.

These sequences typically include the 5' splice site (donor site), branch point sequence, and 3' splice site (acceptor site).

To determine if the intron contains normal consensus splice site sequences, we need to analyze the surrounding sequences.

Unfortunately, the provided sequences do not contain enough context to accurately identify the splice sites.

The splice site sequences are typically characterized by specific nucleotide motifs and consensus sequences (e.g., GT-AG rule).

Without additional context, it is not possible to determine whether the splice sites in the intron conform to the normal consensus sequence.

To fully analyze the splice sites, we would need to examine the genomic DNA sequences upstream and downstream of the intron for potential donor and acceptor sequences, as well as branch point motifs.

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Related Questions

To make solution B, take 0.1 mL of stock A and mix it with 0.9
mL of water. Then, 19 mL of Biuret reagent is added to solution B.
How often was stock A diluted? (To put it another way, compute the
dil

Answers

The dilution of stock A in making solution B was approximately 0.523%.

To find the dilution of stock A in making solution B, we can use the formula: C1V1 = C2V2, where C1 is the initial concentration, V1 is the initial volume, C2 is the final concentration and V2 is the final volume. Let us assume the initial concentration of stock A is 100% or 1.0.C1V1 = C2V2. Initial volume of stock A = V1 = 0.1 mL, Final volume of solution B = V2 = 0.1 mL + 0.9 mL + 19 mL = 19.0 mL + 0.1 mL, Final concentration of stock A = C2, Concentration of Biuret reagent is not given, therefore, we assume it to be 100% or 1.0 mL. Now, applying the formula above: C1V1 = C2V2(1.0)(0.1) = C2(19.0 + 0.1)C2 = 0.1/19.1≈0.00523 or 0.523%. Thus, the dilution of stock A in making solution B was approximately 0.523%. This can also be expressed as 1 part of stock A diluted with 190 parts of water to make 191 parts of solution B.

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A full report of an experiment to test the effect of gravity on the growth of stems and roots. Report observations on the seedling growth. Relate with geotropism.

Answers

Experiment to test the effect of gravity on the growth of stems and roots can be conducted using seedlings.

The experiment will involve observing the growth of the seedlings in two different environments. One environment will be the normal growth environment, which allows for normal plant growth, and the other will be an environment that has altered gravity.

The seedlings will be grown in a container, and the container will be rotated so that the gravity is altered. This will allow the experiment to test the effect of gravity on the growth of the roots and stems.

The purpose of this experiment is to see how the roots and stems of a plant grow in response to gravity.

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(a) Mutations in two different genes (b) Mutations in the same gene 同 1 P AA bb Х aa BB P AA bb X AA bb II ਨੂੰ II 1 Complementation J] Noncomplementation 同 F1 F Aa Bb Genetic mechanism of AA bb complementation Genetic mechanism of noncomplementation Figure 2.21 Locus heterogeneity: Mutations in any one of many genes can cause deafness. (a) Two deaf parents can have hearing offspring if the mother and father are homozygous for recessive mutations in different genes. (b) Two deaf parents with mutations in the same gene may produce all deaf children.

Answers

When a set of parents that are homozygous for recessive mutations in different genes reproduce, two deaf parents can have hearing offspring. Two deaf parents with mutations in the same gene can produce all deaf children. This is due to the locus heterogeneity mechanism where mutations in any one of many genes can cause deafness.

Deafness is a disease that affects hearing. The genetic cause of deafness can be due to mutations in different genes, which can lead to deafness through locus heterogeneity, which is a mechanism where mutations in any one of many genes can cause deafness. When two homozygous recessive parents have mutations in different genes, the cross between them can result in hearing offspring. This is because the mutations are in different genes and therefore are not responsible for the same phenotype, which means there is no complementation between the genes.

The deafness caused by mutations in the same gene leads to the inability to produce a functional protein, resulting in deafness. This is the result of non-complementation because the genes are not able to interact with each other when they are in the same functional pathway. As a result, two deaf parents with mutations in the same gene will produce all deaf children.Therefore, the locus heterogeneity mechanism is responsible for the phenomenon where two deaf parents can have hearing children if the mutations are in different genes.

However, if the mutations are in the same gene, non-complementation occurs, leading to all deaf children. This indicates that the genetic mechanism of complementation and non-complementation can be used to determine whether deafness is caused by mutations in different genes or the same gene.

Deafness is caused by mutations in different genes or the same gene. The genetic mechanism of complementation and non-complementation can be used to determine whether deafness is caused by mutations in different genes or the same gene. When two homozygous recessive parents have mutations in different genes, they can still produce hearing offspring. On the other hand, two deaf parents with mutations in the same gene will produce all deaf children. Therefore, locus heterogeneity is responsible for the former, and non-complementation is responsible for the latter.

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Question 9
Type 1 hypersensitivity involves the immune reactant:
A
IgG
B
TH1
IgE
TCTL
Question 10
Cytokine that causes B cells to differentiate into memory cells
A) IFN gamma
B) IL-21
IL-10
IL-4

Answers

Type 1 hypersensitivity involves the immune reactant IgE, while the cytokine that causes B cells to differentiate into memory cells is IL-21. Below is a brief description of each:Type 1 hypersensitivity:It is also known as anaphylactic or immediate hypersensitivity.

This is a type of immune response where the IgE antibodies that are produced by plasma cells in response to an allergen attach to Fc receptors on the surface of mast cells and basophils. The cross-linking of IgE on mast cells and basophils triggers the release of histamine and other inflammatory mediators, leading to an allergic reaction.

Cytokine that causes B cells to differentiate into memory cells:IL-21 is a cytokine that is produced by activated T helper cells and natural killer T cells. It is involved in the regulation of immune responses and the differentiation of B cells into memory cells and plasma cells. It also plays a role in the differentiation and maintenance of T follicular helper cells. Hence, the cytokine that causes B cells to differentiate into memory cells is IL-21.

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can
you please answer these questions?
What factors determine basal metabolic rate? What is the difference between hunger and appetite? What are the effects of emotions upon appetite?

Answers

Body composition, age, gender, body size, thyroid function are the factors determine basal metabolic rate (BMR).

Factors that determine basal metabolic rate (BMR) include:

Body composition: Lean muscle mass generally increases BMR, as muscle requires more energy to maintain than fat.Age: BMR tends to decrease with age due to a decrease in muscle mass and a slower metabolic rate.Gender: Men typically have a higher BMR than women due to generally higher muscle mass and testosterone levels.Body size: Larger individuals tend to have a higher BMR due to having more body mass to maintain.Thyroid function: Thyroid hormones play a crucial role in regulating metabolism, and any abnormalities in thyroid function can affect BMR.

Hunger refers to the physiological sensation of needing food and is primarily driven by biological factors such as low blood glucose levels and hormonal signals. Appetite, on the other hand, is the desire or craving for food, which can be influenced by factors beyond physiological needs, such as psychological and environmental cues.

Emotions can have various effects on appetite. For some individuals, emotions like stress, anxiety, or sadness can lead to a decrease in appetite, resulting in reduced food intake. In contrast, other individuals may experience an increase in appetite when experiencing certain emotions, leading to emotional eating as a coping mechanism.

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Which statement is correct? a. In pulmonary embolism an embolus travels from a leg to the lungs through the left side of the heart b. The mutated protein responsible for cystic fibrosis secretes sodium ions C. Cystic fibrosis is a common dominant genetic disease d. One quarter of the world's population is infected by Mycobacterium tuberculosis e. Elastase destroys the large airways in emphysema

Answers

The correct statement is e. Elastase destroys the large airways in emphysema.

Elastase is an enzyme that plays a role in the breakdown of elastin, a protein responsible for maintaining the elasticity of lung tissue. In emphysema, there is an imbalance between the production of elastase and the inhibition of its activity, leading to the destruction of the elastic fibers within the lungs. This destruction primarily affects the walls of the alveoli and larger airways, resulting in the loss of structural support and impaired lung function.

Pulmonary embolism, as described in option a, occurs when a blood clot (embolus) travels to the lungs, typically originating from the deep veins of the legs. However, the embolus does not pass through the left side of the heart during this process.

Cystic fibrosis, mentioned in option c, is an autosomal recessive genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. This mutated protein affects chloride ion transport, not sodium ions, leading to the production of thick, sticky mucus in various organs.

Option d, stating that one-quarter of the world's population is infected by Mycobacterium tuberculosis, is not accurate. While tuberculosis is a significant global health issue, the exact percentage of the population affected varies among different regions and populations.

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When a person is performing an aerobic exercise session lasting
30 seconds, the
most appropriate rest period is
answer: 2 minutes
need help on how to do the calculations to get 2 minutes.

Answers

The most appropriate rest period after a 30-second aerobic exercise session is generally recommended to be 2 minutes.

To calculate the most appropriate rest period after a 30-second aerobic exercise session, it's important to consider the nature of the exercise and the individual's fitness level. The general guideline is to have a rest period that allows for recovery and replenishment of energy stores.

Typically, aerobic exercise primarily relies on the aerobic energy system, which utilizes oxygen to generate energy. During intense exercise, the body consumes oxygen at a faster rate than it can replenish, leading to an oxygen debt. The rest period allows for the repayment of this oxygen debt and the restoration of energy stores, such as ATP and glycogen.

While the specific duration of the rest period can vary based on factors like individual fitness, exercise intensity, and training goals, a commonly recommended guideline is a rest period that is several times longer than the exercise duration. For a 30-second aerobic exercise session, a rest period of 2 minutes is often considered appropriate.

This 2-minute rest period allows for the replenishment of oxygen stores, clearance of metabolic byproducts like lactate, and recovery of energy systems. It provides a sufficient time window for the body to prepare for subsequent exercise bouts and helps maintain performance throughout the workout.

It's important to note that individual responses to exercise and optimal rest periods can vary. Consulting with a fitness professional or personal trainer can provide personalized guidance based on specific fitness goals, abilities, and exercise program design.

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24. a properly tied tourniquet a. permits arterial flow and blocks venous flow b. blocks arterial and venous flow c. prevents back flow d. permits venous flow and block arterial flow

Answers

A properly tied tourniquet blocks arterial and venous flow.

When a tourniquet is correctly applied, it exerts pressure on the underlying tissues, compressing the blood vessels and restricting blood flow. This compression is necessary to temporarily stop bleeding or limit blood loss during certain medical procedures or in emergency situations.

By blocking both arterial and venous flow, a properly tied tourniquet effectively prevents blood from entering or leaving the area beyond the applied point. This helps to create a bloodless field for procedures such as limb amputations or controlling severe bleeding.

However, it is essential to note that tourniquets should only be used as a last resort and for a limited period since prolonged application can lead to tissue damage or other complications. Medical professionals should be consulted for proper tourniquet use and monitoring.

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Innate forms of behavior:
A) Unconditioned reflexes and their
classification,significance
B) Instincts, their types: phase origin of instinctive
activity, significance
C) The motivations, their phy

Answers

Innate forms of behavior: A) Unconditioned reflexes are the automatic response of an animal to a stimulus and their classification are autonomic reflexes, somatic reflexes, and complex reflexes, B) Instincts behaviors that are present in animals from birth. There are two types of instincts: fixed action patterns and innate releasing mechanisms. C) The motivations are internal factors that cause an animal to act in a certain way. There are three types of motivations: hunger, thirst, and sex,

Innate forms of behavior refer to natural behaviors that animals are born with, these behaviors are independent of any previous experience. There are three types of innate behaviors: unconditioned reflexes, instincts, and motivations. Unconditioned reflexes are the automatic response of an animal to a stimulus, these reflexes are classified into three categories: autonomic reflexes, somatic reflexes, and complex reflexes. Autonomic reflexes include heart rate and digestive system. Somatic reflexes involve skeletal muscles.

Complex reflexes are more complicated and involve a combination of autonomic and somatic reflexes. The significance of unconditioned reflexes is that they help animals react to stimuli in their environment, allowing them to survive and reproduce. Instincts are behaviors that are present in animals from birth. There are two types of instincts: fixed action patterns and innate releasing mechanisms. Fixed action patterns are behaviors that are unchangeable and are triggered by a specific stimulus. Innate releasing mechanisms are neural circuits that detect the presence of a specific stimulus and cause an animal to perform a specific behavior.

The phase origin of instinctive activity refers to the sequence of behaviors that make up a specific instinct. The significance of instincts is that they help animals survive and reproduce by providing them with the ability to perform specific behaviors without having to learn them. Motivations are internal factors that cause an animal to act in a certain way, there are three types of motivations: hunger, thirst, and sex. Hunger is the motivation to eat, thirst is the motivation to drink, and sex is the motivation to mate, the physiological mechanisms behind these motivations are regulated by the hypothalamus in the brain. So therefore these innate form of behavior form unconditioned reflexes, instincts, and motivations.

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Protein X folds into its native conformation only when protein Z is present. However, protein Z can fold to its native conformation without the presence of protein X.
It can be said that protein Z acts as ________ for protein x
a. to messenger mRNA
b. denaturing agent
c. ligand
d. ribosome
e. chaperonin

Answers

Protein Z acts as chaperonin for protein X. A chaperonin is a protein molecule that can assist other proteins in folding into their native state.

When a protein is synthesized, it is not yet in its functional state, so it must fold into its final, three-dimensional conformation.

Chaperonins can be found in most living cells and play a crucial role in protein folding.When protein X folds into its native conformation only when protein Z is present, this indicates that protein Z acts as a chaperonin for protein X.

This means that protein Z assists protein X in folding into its final, three-dimensional conformation.

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23) When a carbon containing molecule is reduced, the molecule:
A) gains electrons.
B) loses electrons.
C) gains potential chemical energy.
D) loses potential chemical energy.
E) A and C
F) B and D
24) Which of the following replaces electrons lost by Photosystem II in the light reaction?
A) NADPH
B) The Water-Splitting Reaction
C) ATP
D) Proton Pumps

Answers

23) When a carbon-containing molecule is reduced, it gains electrons. 24) The water-splitting reaction replaces electrons lost by Photosystem II in the light reaction

Reduction in chemistry refers to a chemical reaction that occurs when electrons are gained. When a molecule is reduced, it gains potential chemical energy and becomes less oxidized.

This is the result of a reduction reaction, which is a type of chemical reaction in which an atom gains electrons and decreases its oxidation state.

Electrons are transferred from one atom to another in a reduction reaction. The reduction reaction may be represented as the addition of electrons to a chemical entity, the addition of hydrogen, or the removal of oxygen. When a carbon-containing molecule is reduced, the molecule gains potential chemical energy.

Hence, the correct answer is option A.

24) The water-splitting reaction replaces electrons lost by Photosystem II in the light reaction. The water-splitting reaction, which takes place on the thylakoid membranes of plants, is the source of the oxygen that is released during photosynthesis.

It's also the source of the electrons that are required to replace those lost by Photosystem II in the light reaction. Water is the raw material for the water-splitting reaction.

The splitting of water molecules by light into hydrogen ions (H+) and oxygen gas (O2) is called the water-splitting reaction. The oxygen released by the reaction is used in cellular respiration by organisms that breathe oxygen.

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Group Project - Health and Biology B The human field of view is slightly more than 180° horizontally, which means we are capable of noticing things positioned very slightly behind us and far to our left, in front of us, and very slightly behind us and far to our right. However, the left-most and right-most ends of this vision are only covered by one eye. Our binocular field of view, the portion that both eyes can see, is only 114° horizontally. Your lab is putting a mural on the side of the building. The mural should be as big as possible while still being fully viewable by both eyes in a single glance from 20ft away. How wide should you make the mural?

Answers

To ensure that the mural is fully viewable by both eyes in a single glance from 20ft away, it should be designed to fit within the binocular field of view, which is 114° horizontally.

The human binocular field of view is the portion of our visual field that can be seen by both eyes simultaneously. In this case, we need to determine the maximum width of the mural that can be seen within the binocular field of view from a distance of 20ft.

The binocular field of view is approximately 114° horizontally. This means that if the mural is wider than 114°, we would need to move our eyes or head to see the entire width of the mural. To ensure that the mural can be viewed in a single glance, it should not exceed the width of the binocular field of view.

To calculate the width of the mural, we need to determine the angle subtended by the mural at the viewing distance of 20ft. Using trigonometry, we can use the tangent function to calculate this angle. Assuming the mural is positioned at eye level, we can consider the distance between the eyes to be negligible.

Let's assume that the width of the mural is represented by "w." Using the tangent function, we can calculate the angle as tan(114/2) = (w/2) / 20. Solving for "w," we get w = 2 * 20 * tan(114/2).

By evaluating this equation, we can determine the maximum width of the mural that can be fully viewable within the binocular field of view from a distance of 20ft.

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The main topic or concept will be "CELLULAR RESPIRATION and PHOTOSYNTHESIS". From this main concept, you will branch out and link to the other concepts.
Link the concepts with connectors and labels if there are concepts that are linked between units. Note: the connecting arrows do not have to be linear, they may be curved.

Answers

Cellular respiration and photosynthesis are interdependent processes that are crucial for energy transfer, gas exchange, and the cycling of carbon and oxygen in the biosphere. They form a vital link in the overall functioning of ecosystems and the sustainability of life on Earth.

Cellular respiration and photosynthesis are essential processes that occur in living organisms, particularly in plants and some microorganisms. Cellular respiration is the process by which cells break down organic molecules, such as glucose, to generate ATP, the primary energy currency of cells. During cellular respiration, oxygen is consumed, and carbon dioxide and water are produced as byproducts.

Photosynthesis, on the other hand, is the process by which plants, algae, and some bacteria convert light energy into chemical energy in the form of glucose. In this process, carbon dioxide and water are taken in, and using the energy from sunlight, they are converted into glucose and oxygen is released as a byproduct.

These two processes are interconnected as they participate in the cycling of carbon and oxygen in the biosphere. The oxygen produced during photosynthesis is used in cellular respiration, while the carbon dioxide produced during cellular respiration is used in photosynthesis. This exchange of gases is crucial for the maintenance of atmospheric composition and the sustenance of life on Earth.

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A Cdc42N17 mutant cannot bind GTP. From your General Cell Biology knowledge, predict the effect this has on Cdc42 activity. a, Cdc42 activity will not be affected. b. Cdc42 will be constitutively active. c. Cdc42 can not be activated. Question 16 A Cdc42V12 mutant cannot hydrolyze GTP. From your General Cell Biology knowledge, predict the effect this has on Cdc42 activity. a. Cdc42 can not be activated. b. Cdc42 activity will not be affected. c. Cdc42 will be constitutively active.

Answers

Cdc42N17 mutant: Cdc42 activity is impaired due to the inability to bind GTP. Cdc42V12 mutant: Cdc42 activity is enhanced and constitutively active due to the inability to hydrolyze GTP.

The Cdc42N17 mutant, which cannot bind GTP, will have impaired activity since GTP binding is necessary for its activation. GTP binding induces a conformational change in Cdc42, allowing it to interact with downstream effectors and regulate cellular processes such as cell division, cytoskeletal organization, and cell polarity.

Without GTP binding, Cdc42 cannot undergo the necessary conformational change and thus remains inactive. On the other hand, the Cdc42V12 mutant, which cannot hydrolyze GTP, will have constitutively active activity. The inability to hydrolyze GTP results in the prolonged activation of Cdc42, leading to sustained signaling and altered cellular responses.

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Mark whether each of these statements are True or False.
a. A ΔG of zero means the system is at equilibrium.
True or False
b. A positive ΔG tells us about the rate of a reaction. True or False
c. A negative ΔG is considered non- spontaneous/unfavorable. True or False
d. Enzymes increase the activation energy of reactions and thereby make reactions to occur faster. True or False
e. Enzymes alter the free energy change of a reaction.True or False

Answers

Enzymes lower the activation energy of reactions, making them occur faster, but they do not alter the free energy change or the thermodynamic favorability of a reaction. The sign of ΔG determines whether a reaction is spontaneous (negative ΔG) or non-spontaneous (positive ΔG).

a. False. A ΔG of zero means that the system is at a state of equilibrium. At equilibrium, the forward and reverse reactions occur at the same rate, and there is no net change in the concentrations of reactants and products. However, this does not mean that the system is static or no longer undergoing any reaction.

b. False. A positive ΔG indicates that the reaction is non-spontaneous and requires an input of energy to proceed. It does not provide information about the rate of the reaction. The rate of a reaction is determined by factors such as the presence of a catalyst or the concentration of reactants.

c. False. A negative ΔG indicates that the reaction is spontaneous and favorable, as it releases energy. An exergonic reaction with a negative ΔG occurs spontaneously without the need for an input of energy. It is important to note that the thermodynamic favorability of a reaction is determined by the ΔG value, not its sign.

d. False. Enzymes do not increase the activation energy of reactions. In fact, enzymes lower the activation energy barrier, making it easier for reactions to occur by providing an alternative pathway with a lower energy requirement. By lowering the activation energy, enzymes increase the rate of the reaction without being consumed in the process.

e. False. Enzymes do not alter the free energy change (ΔG) of a reaction. They do not affect the thermodynamic favorability of a reaction or change the difference in free energy between reactants and products. Enzymes only facilitate the conversion of reactants to products by lowering the activation energy barrier, but they do not impact the overall change in free energy.

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Label the veins of the head and neck as seen from an anterior view. Subclavian v. Left brachiocephalic V. 111 Zoom External jugular v. ne Azygos v. Internal jugular v. Reset

Answers

When viewed from the front, the veins of the head and neck can be identified as follows: the subclavian vein, left brachiocephalic vein, external jugular vein, azygos vein, and internal jugular vein. These veins play a crucial role in draining blood from the upper limbs, head, face, and neck.

From an anterior view, the veins of the head and neck can be labeled as follows:

1. Subclavian vein: The subclavian vein is located on both sides of the neck and forms a continuation of the axillary vein.

It receives blood from the upper limbs and combines with the internal jugular vein to form the brachiocephalic vein.

2. Left brachiocephalic vein: The left brachiocephalic vein is a large vein formed by the union of the left subclavian vein and the left internal jugular vein.

It is located on the left side of the neck and carries deoxygenated blood from the upper limbs and head.

3. External jugular vein: The external jugular vein is a superficial vein that can be seen on the side of the neck. It drains blood from the scalp and face and typically joins the subclavian vein.

4. Azygos vein: The azygos vein is a major vein located in the posterior mediastinum (chest region). While it is not visible from an anterior view, it is still an important vein to mention.

It receives blood from the thoracic and abdominal walls and contributes to the drainage of the upper body.

5. Internal jugular vein: The internal jugular vein is a large vein located deep within the neck. It receives blood from the brain, face, and neck, and combines with the subclavian vein to form the brachiocephalic vein.

It's worth noting that labeling the veins accurately requires a detailed understanding of human anatomy and the ability to visualize the specific structures.

It is always recommended to consult an anatomical diagram or seek professional guidance when studying or identifying veins.

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1. Which of the following is TRUE about digestive microbiomes? a. Microbiomes can only include non-pathogenic bacteria
b. The presence of a microbiome reduces the number of calories an animal can extract from food
c. Microbiome members can only be bacteria
d. Microbiomes are only found in or on animals
e. None of the above

Answers

The statement "None of the above" is the correct answer regarding digestive microbiomes. The correct answer is option e.

Microbiomes can include both pathogenic and non-pathogenic bacteria, contradicting option a.

In fact, the presence of a microbiome is known to enhance the host's ability to extract calories from food, challenging option b. Microbiome members are not limited to bacteria alone; they can also include archaea, viruses, fungi, and other microorganisms, refuting option c. Furthermore, microbiomes are not exclusive to animals; they can exist in various environments such as soil, water, and plants, disproving option d.

Therefore, the correct choice is e. None of the above, as none of the statements accurately describe digestive microbiomes.

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c) Why does it appear that increasing levels of rho protein lowers the rate of incorporation of nucleotides into RNA? Explain by describing what's happening at the molecular level. innove the riho at

Answers

The

increasing levels of rho protein will lower the rate of incorporation of nucleotides into RNA.

Rho protein is a transcription termination factor in prokaryotes that can stop the process of transcription. When rho protein levels are increased, it results in a decrease in the rate of incorporation of nucleotides into RNA.

The rho protein will then push the RNA polymerase off the DNA template, releasing the newly synthesized RNA molecule and terminating transcription. However, if the level of rho protein increases, it will bind to the RNA transcript more often, leading to premature termination of RNA synthesis.

This will result in incomplete RNA transcripts, which are less efficient in protein synthesis and lead to a decrease in the rate of incorporation of nucleotides into RNA. The

increasing levels of rho protein will lower the rate of incorporation of nucleotides into RNA.

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(D) True or false about the following statements on Insulin ligands, animal growth, and animal size
A. DILPs are produced by certain neurons in Drosophila brain, which are released into hemolymph to coordinately regulate organ growth and larvae growth. The levels of DILPs in hemolymph will correlate with faster animal growth rate and larger animal sizes.
B. The levels of DILPs released in the hemolymph are impacted by nutrient levels. Adding more nutrients in the regular fly food will lead to higher levels of DILPs in the hemolymph and larger animal sizes.
C. Flies that grow under very poor nutrient conditions will have much lower levels of DILPs in their hemolymph and will take longer to grow and develop into adults of smaller sizes.
D. Flies that grow under low temperature conditions (18°C) will have lower levels of DILPs in their hemolymph. These flies will take longer to grow but the adult sizes are not significantly affected.

Answers

Insulin ligands, animal growth, and animal size are true or false:D. Flies that grow under low temperature conditions (18°C) will have lower levels of DILPs in their hemolymph. These flies will take longer to grow but the adult sizes are not significantly affected.The statement is True.Explanation:Insulin is a peptide hormone that plays a crucial role in glucose homeostasis, lipid metabolism, and the growth and development of animals. Insulin-like peptides (DILPs) are produced by a set of neurons in the Drosophila brain, and their release into the hemolymph regulates organ and larval growth.

The levels of DILPs in the hemolymph are determined by nutrient levels. In Drosophila, higher nutrient levels in the food result in higher levels of DILPs in the hemolymph, which leads to increased growth rate and animal size.In flies that grow under very poor nutrient conditions, there are much lower levels of DILPs in their hemolymph, and they take longer to grow and develop into smaller adult sizes.

Flies that grow under low-temperature conditions have lower levels of DILPs in their hemolymph. These flies take longer to grow, but the adult size is not significantly affected. Therefore, the statement "D. Flies that grow under low temperature conditions (18°C) will have lower levels of DILPs in their hemolymph. These flies will take longer to grow but the adult sizes are not significantly affected" is True.

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Several viruses, including many in the herpesvirus family, have been found to encode proteins that are homologs of Bcl-2. Why would the expression of Bcl-2 homologs be beneficial to a virus infecting a host cell? a. It can induce apoptosis in the infected host cell. b. It will have no effect on the infected host cell. c. It can induce apoptosis in responding cytotoxic T lymphocytes. d. It will block apoptosis in the infected host cell. e.It can block block death receptors from engaging cytotoxic T lymphocytes.

Answers

These homologs have the potential to block apoptosis, allowing the virus to replicate and spread throughout the host cell. So, the correct option is d.

Several viruses, including many in the herpesvirus family, have been found to encode proteins that are homologs of Bcl-2. The expression of Bcl-2 homologs be beneficial to a virus infecting a host cell because it will block apoptosis in the infected host cell. The correct option is d. It will block apoptosis in the infected host cell.What is Bcl-2?B-cell lymphoma 2 (Bcl-2) is an anti-apoptotic protein encoded by the BCL2 gene in humans.

The role of Bcl-2 is to control apoptosis (programmed cell death). When apoptosis is triggered by an internal or external signal, it prevents it from happening. The herpesvirus family's viruses contain homologs of Bcl-2, which are proteins that have the same or similar amino acid sequence as Bcl-2.Therefore, these homologs have the potential to block apoptosis, allowing the virus to replicate and spread throughout the host cell. So, the correct option is d. It will block apoptosis in the infected host cell.

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How does salting out work?
a. High levels of salt interact with a large portion of the water dipoles, thus decreasing the number of interactions of the protein with water.
b. The salt enhances protein-water interactions, increasing the solvation of the protein.
c. Increasing the salt concentration permits the hydrophobic portions of individual protein molecules to invert position to the protein interior, making the proteins more soluble in aqueous solution.
d. Reducing the ionic concentration in the protein solution allows charged groups on the proteins to interact more readily with the water solvent molecules.

Answers

The correct answer is option A, as high levels of salt interact with water dipoles, reducing the interactions between water and proteins.

Salting out is based on the principle that high salt concentrations can reduce the solubility of proteins in aqueous solutions. When salt is added to a protein solution, it interacts with a large portion of the water dipoles present. This interaction disrupts the protein-water interactions, leading to a decrease in protein solubility.

Option A is the correct answer because the high levels of salt decrease the number of interactions between the protein and water molecules. This reduction in protein-water interactions causes the proteins to aggregate and precipitate out of solution. As a result, the proteins become less soluble and can be easily separated from the solution by centrifugation or filtration.

Options B, C, and D are incorrect. Option B suggests that salt enhances protein-water interactions, which is opposite to what actually happens during salting out. Option C refers to the inversion of hydrophobic portions of proteins, which is unrelated to the salting-out phenomenon. Option D suggests that reducing ionic concentration enhances protein-water interactions, which is also contrary to the salting-out process.

Therefore, option A accurately describes how salting out works by decreasing the interactions between proteins and water through the interaction of salt with water dipoles.

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Patient X is a 75 year old female who had a cystocele repair 10 days ago. Upon admission to the hospital, her urine culture showed > 100,000 CFU/mL of an E. coli strain susceptible to all tested antibiotics. She was given oral cephalexin for 7 days post-operation and was discharged after day 3. Patient X begins to exhibit diarrhea for 3 days, after 10 days post-op. Patient complained of loose watery stools, showing no blood, abdominal cramps and emesis. Patient's stats are pulse rate of 95/min, respiration rate of 25/min, temp is 39 degrees Celsius, and blood pressure is 117/54 mm Hg. WBC count is normal, but shows many (54%) polymorphonuclear cells (immature). Patient X's electrolytes, lipase, liver enzymes and examination were all normal. Cultures returned negative for Salmonella, Shigella, Yersinia, and Campylobacter spp. 1. What microbe is causing Patient X's diarrhea? 2. What predisposing factors did Patient X have for this infection?

Answers

Patient X has diarrhea caused by C. difficile infection.  The factors that predisposed Patient X for this infection are antibiotic use and age. Here's a detailed answer to your question:

Answer 1:Patient X has diarrhea caused by Clostridioides difficile (C. difficile) infection. C. difficile infection is a bacterial infection that causes severe diarrhea. C. difficile bacteria naturally occur in the human gut and do not cause illness in healthy individuals. However, when the balance of good and harmful bacteria in the gut is disrupted, C.

difficile bacteria can multiply and produce toxins that cause diarrhea. Antibiotic use is the most common cause of C. difficile infection. Antibiotics disrupt the gut microbiota and kill the good bacteria that normally keep C.

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What is the term for the process whereby the synthesis of ATP is coupled to the conversion of NADH or FADH₂ to NAD* or FAD? a) Oxidative phosphorylation. b) Substrate-level phosphorylation. c) Substrate level oxidation. d) Oxidative reduction.

Answers

The term for the process whereby the synthesis of ATP is coupled to the conversion of NADH or FADH₂ to NAD+ or FAD is a) Oxidative phosphorylation.

Oxidative phosphorylation is a metabolic pathway that occurs in the inner mitochondrial membrane of eukaryotic cells or the plasma membrane of prokaryotic cells. It is the final step of cellular respiration and involves the transfer of electrons from NADH and FADH₂ to the electron transport chain (ETC). As the electrons pass through the ETC, their energy is used to pump protons (H+) across the membrane, creating an electrochemical gradient. The flow of protons back across the membrane through ATP synthase drives the synthesis of ATP from ADP and inorganic phosphate. This process is called oxidative phosphorylation because it couples the oxidation of NADH and FADH₂ with the phosphorylation of ADP to form ATP.

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The hormone that is created in the ovary right after ovulation
takes place
A. HCG
B. estrogens
C. androgens
D. progesterone

Answers

The hormone which is produced in the ovary immediately following ovulation is progesterone.

What is ovulation?

Ovulation is the process of releasing an egg from a woman's ovary. This egg passes through the fallopian tube and enters the uterus, where it may or may not be fertilized by sperm. After ovulation, the empty follicle that released the egg becomes the corpus luteum, which produces progesterone.

Progesterone is a hormone that prepares the uterus for implantation of a fertilized egg. If pregnancy does not occur, the corpus luteum degenerates, and progesterone levels drop, leading to the shedding of the uterine lining and the onset of menstruation.

Therefore, option D (progesterone) is the hormone that is produced in the ovary immediately following ovulation.

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BLOOD COMPOSITION QUESTIONS 1. Fill in the blank for the following statements about blood composition a. The blood consists of 55% of plasma and 4596 of formed elements. b. Normal hematocrit readings

Answers

In blood composition, plasma constitutes 55% of the total volume, while formed elements make up 45%. Normal hematocrit readings vary depending on the individual's age, sex, and health status.

Blood is composed of two main components: plasma and formed elements. Plasma is the liquid portion of blood, accounting for approximately 55% of the total volume. It is a yellowish fluid that consists mainly of water, along with various proteins, electrolytes, hormones, and waste products.

Formed elements refer to the cellular components of blood, including red blood cells (erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes). These formed elements make up approximately 45% of the blood volume.

Hematocrit is a measurement that represents the percentage of red blood cells in the total blood volume. Normal hematocrit readings can vary depending on factors such as age, sex, and overall health. In adult males, the normal range is typically between 40% and 52%, while in adult females, it is between 37% and 48%. These values can differ in children, pregnant women, and individuals with certain medical conditions.

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5. You are following a family that has a reciprocal translocation, where a portion of one chromosome is exchanged for another, creating hybrid chromosomes. In some cases of chronic myelogenous leukemia, patients will have a translocation between chromosome 9 and 22, such that portions of chromosomes 9 and 22 are fused together. You are choosing between performing FISH and G-banding, which technique is best used to find this translocation, and why did you choose this technique?
6. What type of nucleotide is necessary for DNA sequencing? How is it different structurally from a deoxynucleotide, and why is this difference necessary for sequencing? Below is a Sequencing gel. Please write out the resulting sequence of the DNA molecule. Blue = G, Red C, T=Green, A = Yellow (Please see below for the gel).

Answers

The best technique to detect the translocation in the family with reciprocal translocation would be Fluorescence In Situ Hybridization (FISH).

FISH is specifically designed to detect chromosomal abnormalities and rearrangements, such as translocations. It uses fluorescently labeled DNA probes that can bind to specific target sequences on the chromosomes. In the case of the translocation between chromosomes 9 and 22, FISH probes can be designed to specifically bind to the hybrid chromosomes formed by the fusion of these two chromosomes. By visualizing the fluorescent signals under a microscope, FISH allows for the direct detection and localization of the translocation event.

The nucleotide necessary for DNA sequencing is a deoxynucleotide triphosphate (dNTP). Structurally, a deoxynucleotide consists of a deoxyribose sugar, a phosphate group, and one of the four nitrogenous bases: adenine (A), cytosine (C), guanine (G), or thymine (T). The key difference between a deoxynucleotide and a nucleotide used in RNA (ribonucleotide) is the absence of an oxygen atom on the 2' carbon of the sugar in deoxynucleotides. This difference makes deoxynucleotides more stable and less susceptible to degradation.

During DNA sequencing, the incorporation of dNTPs is crucial. Each dNTP is complementary to the template DNA strand at a specific position. The DNA polymerase enzyme incorporates the appropriate dNTPs according to the template sequence, and the sequencing reaction proceeds by terminating the DNA synthesis at different points. By using dideoxynucleotides (ddNTPs) that lack the 3'-OH group necessary for further DNA elongation, the resulting DNA fragments can be separated by size using gel electrophoresis, as shown in the sequencing gel provided. The sequence of the DNA molecule can be determined based on the order of the colored bands, with blue representing G, red representing C, green representing T, and yellow representing A.

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2. DISCUSS THE Q-ANGLE IN PATELLOFEMORAL STABILITY?

Answers

The Q-angle is defined as the angle formed by the intersection of two lines, one from the center of the patella to the tibial tubercle and the other from the anterior superior iliac spine to the center of the patella.

In patellofemoral stability, the Q-angle is significant because it measures the alignment of the quadriceps muscles, patella, and tibia. When a large Q-angle is present, it can cause the patella to become misaligned and cause pain in the patellofemoral joint. The Q-angle can be altered by various factors, including pelvic width, femoral anteversion, and tibial torsion.

Patellofemoral stability is a complex and dynamic interaction between the soft tissues, bones, and biomechanical forces acting on the knee. The Q-angle is one factor that can contribute to patellofemoral stability and should be evaluated in patients with knee pain or instability.

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Consider an animal cell that has twelve chromosomes (six pairs of homologous chromosomes) in G₁ phase. Indicate how many of each of the following features the cell will have at mitotic prophase. Write what you think the number is in the box (eg. 12 not twelve). There are Sister chromatids present in this cell. At the end of mitosis there will be Chromosomes in each daughter cell. The number of Kinetochores present in the cell is You would find There are Centromeres in this cell. there are Centrioles present.

Answers

The  Sister chromatids are present in this cell, but they have not yet been produced through DNA replication because the cell is in the G1 phase. The result would be 0 as a result.

The cell has 12 chromosomes in G1 phase, and during mitosis, the chromosomes multiply and separate. At the completion of mitosis, there are two copies of each chromosome in each daughter cell. There will be 12 chromosomes in each daughter cell after the conclusion of mitosis.The number of kinetochores in the cell is zero since each sister chromatid contains a kinetochore and the cell is in the G1 phase without duplicated sister chromatids.Centromeres in this cell: There is one centromere on each of the cell's 12 chromosomes. Consequently, there

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Describe the adaptations to flight displayed by modern birds.
(discuss wings, advanced circulatory and respiratory systems, as
well as lighter bone structure)

Answers

The adaptations that allow birds to fly are a combination of lightweight, strong bones, advanced respiratory and circulatory systems, and specialized wings and feathers:

1. Advanced circulatory and respiratory systems:

Birds have an efficient respiratory system and advanced circulatory system. A bird's respiratory system is more efficient than a mammal's respiratory system because it can extract more oxygen out of the air. The respiratory system of birds includes a series of air sacs that extend throughout the body and are connected to the lungs.

2. Wings:

Flight feathers cover a bird's wings and tail. These feathers provide lift, reduce drag, and control the bird's movement during flight. The shape of the bird's wings is adapted to its specific mode of flight. Some birds have long, pointed wings for soaring, while others have short, rounded wings for quick, agile movements.

3. Lighter bone structure:

The bones of birds are light and strong, which is necessary for flight. Bird bones are hollow, and many of the bones have air sacs that are connected to the respiratory system. This makes the bones lighter, which makes it easier for the bird to fly. Furthermore, birds have fewer bones in their bodies than mammals, which also contributes to their lighter weight.

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What precautions should be taken after a patient is given a pacemaker. (surgery)
care after the surgery in daily life
tips and just share helpful things thansk

Answers

After a patient undergoes pacemaker surgery, several precautions should be taken to ensure proper care and minimize complications.

These include avoiding certain activities that may interfere with the pacemaker's functioning, monitoring and maintaining good wound care, and following the guidelines provided by the healthcare team. It is important to consult with healthcare professionals for personalized advice based on the patient's specific condition.

1. Activity Restrictions: Patient with pacemakers should avoid activities that involve strong electromagnetic fields or that may cause direct trauma to the pacemaker site. This includes avoiding close proximity to devices such as MRI machines, metal detectors, and powerful magnets. Contact sports and activities that carry a risk of impact or injury should also be approached with caution.

2. Wound Care: Proper care of the surgical incision site is crucial to prevent infection. Patients should keep the area clean and dry, follow any specific wound care instructions provided by the healthcare team, and monitor for signs of infection such as redness, swelling, or drainage.

3. Medication and Follow-up: Patients should take prescribed medications as directed, including any anticoagulants or antiplatelet drugs, and attend regular follow-up appointments to ensure the pacemaker is functioning properly.

4. Lifestyle Considerations: It may be advisable for patients to avoid prolonged exposure to extreme heat or cold, as temperature fluctuations can affect the pacemaker's performance. It is also important to inform healthcare providers and airport security personnel about the presence of a pacemaker before undergoing medical procedures or passing through security checkpoints.

5. Education and Support: Patients and their caregivers should receive thorough education and support from the healthcare team regarding the care and maintenance of the pacemaker, as well as what to do in case of emergencies or concerns. Being knowledgeable about the device and having access to appropriate resources can contribute to better post-surgery care and overall well-being.

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