A group of scientists were using CRISPR to treat a mouse with cystic fibrosis, a disease caused by a defective Cl- channel protein called CFTR. The most common cause of cystic fibrosis in humans is the absence of a phenylalanine, an amino acid, in the CFTR protein, which prevents CFTR from achieving the correct 3-D structure.If the scientists wished to repair this mutation, how would they alter the mutated CFTR gene before using CRISPR to insert the DNA into the mice

Answers

Answer 1

Answer:

they can insert the codon that encodes phenylalanine by CRISPR-Cas mediated homology-directed repair

Explanation:

The CRISPR (clustered regularly interspaced short palindromic repeats,)-Cas (CRISPR-associated protein) is a versatile technology for genome editing which has been developed by adapting a natural defense mechanism found in prokaryotic organisms. The CRISPR-Cas genome editing tool can be used to edit DNA sequences in eukaryotic genomes (for example, in human genomes) and thus restore gene function (in this case, the CFTR gene function). The system consists of a Cas protein that cleaves the DNA at specific genomic sites and a single guide RNA (sgRNA) that guides the Cas protein at specific genomic locations. CRISPR-Cas mediated homology-directed repair refers to the process where Cas cuts the DNA to triggers the homologous recombination (HR) DNA repair pathway, by which it is possible to insert new genetic sequences at specific genomic sites (in this case, the codon that encodes phenylalanine within the specific CFTR gene).


Related Questions

D. Explain the relationship between the pulse recorded in the finger and heart muscle contraction ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ E. Which components of the cardiac cycle (atrial systole and diastole, ventricular systole and diastole) are discernible in the pulse tracing? ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Answers

Answer:

D. Heart contraction and pulse are connected with one another.

E. Atrial diastole and ventricle diastole.

Explanation:

Heart construction and pulse of an individual are connected as one can hear the pulse every time contraction of the heart is completed. Pulse can be understood by this that it represents the number of the contractions is completed by heart each time.

In the pulse tracing, there are two parts of the heart that are discernible which represents the contraction of the heart that are Atrial diastole and ventricle diastole.

The relationship between the pulse recorded in the finger and heart muscle contraction is that both are connected to each other. But, the analysis and processes are different from one another.

What is Muscle Contraction?

A process leading to shortening and/or development of tension in muscle tissues that means the thin filaments slide over the thick filaments and tension is generated between one another is called Muscle contraction.

The pulse recorded in the finger is a signal for flowing the blood throughout the body, while the process of heart muscle contraction signals for electrical conductivity. Both the functions are connected with each other and acquired by cardiac activities.

The components of the cardiac cycle that are discernible in the pulse tracing are Ventricular systole (contraction) and ventricular diastole (relaxation).

Therefore, both the pulse recorded in the finger and heart muscle contraction interconnected with one another and acquired by cardiac activity.

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What is the complementary DNA strand from the following DNA
template:
GGTACTAGCCATATCG
A- CCATGATCGGTATAGC
B- OTTATGATCGGTATAGC
C- CCAUGAUCGGUAUAG
D- OGGTACTAGCCATATCG

Answers

Avia una pajarito q comia pan y se murio por culpa del pan pobre pajaro

Answer:

The third one

Explanation:

Hey There!

Just so you know when you try to find the complementary base strand you put the letter of the nucleotide that it pairs with

Guanine pairs with Cytosine

Thymine Pairs with Adenine

Adenine Pairs with Uracil

Cytosine Pairs with Guanine

If you have anymore questions feel free to ask :)

Which of the following steps in the water cycle represents a change in the state of
matter?
a. Condensation
b. Evaporation
c. Sublimation
d. All of the above

Answers

The water cycle is the path that all water follows as it moves around our planet. On Earth, you can find water in all three states of matter: solid, liquid and gas. Liquid water is found in Earth's oceans, rivers, lakes, streams—and even in the soil and underground.

Jill has quite a menagerie of pets and she regularly has to deal with minor animal injuries. So when Johnnie, her rooster, spikes Jessie, her collie, on the muzzle with one of his long leg spurs, Jill knows what to do. She reaches for the hydrogen peroxide and thoroughly floods Jessie's puncture wound with the liquid. Jill observes, as always, that the hydrogen peroxide causes heavy fizzing at the site of the wound. She repeats the procedure several times a day over the next week, carefully inspecting the wound for signs of infection. The injury heals without complications and Jessie has a new respect for roosters.
a) What is hydrogen peroxide and how does it help to reduce the infection?
b) Why is hydrogen peroxide especially useful for treating puncture wounds?
c) What causes the fizzing associated with hydrogen peroxide use?

Answers

Answer:

a.) Hydrogen peroxide are highly reactive oxygen compounds used as an antiseptic

b.) Used in treating punctured wounds due to the nature of the wound which helps microorganisms to thrive.

c.) Fizzing associated with hydrogen peroxide use is due to presence of the enzyme catalase.

Explanation:

Hydrogen peroxide is a chemical compound which is made up of two hydrogen atoms and two oxygen atoms. It is powerful oxidizing agent which takes part in biological reactions. This is possible as it can remove electrons from other biological molecules which can damage other cellular components. It is used an an antiseptic to reduce infection because, being a powerful oxidizing agent, it has the ability to react with cell wall of these microorganisms through removal of electrons thereby causing damage to it.

Punctured wounds are narrow in structure which allows obligate anaerobic organisms to thrive because of reduced oxygen flow in the wound. Hydrogen peroxide can effectively eliminate these organisms which are most likely to cause infection in that site because they lack the enzyme called catalase.

Catalase is an enzyme which is present in living cells except obligate anaerobic organisms. It converts hydrogen peroxide into water and oxygen. The fizzing noticed by Jill while cleaning the wound with hydrogen peroxide is due to the catalase in Jill's own cells which converts the hydrogen peroxide to water and oxygen. When oxygen is given off, fizzing reactions are seen at the surface of the wound.

Groundwater is an important source of drinking water especially in Florida. What is the underground reservoir where water is found?

Answers

The main source of water for most of us in northeast and central Florida comes from underground aquifers. Aquifers can be thought of as vast underground, porous rocks that hold water and allow water to move through the holes within the rock.

Select the correct answer.
Lily feels tired and weak in the evenings after work. Before starting her chores, she eats dinner. Which characteristic of life does Lily exhibit?
A.
growth
B.
need for energy
C.
organization
D.
regulation

Answers

i would say need for engery? because when you work a lot, you need that engery. she regains it by eating dinner then wasting more energy on chores. brainliest?

A dwarf planet flies towards the sun and orbits it. As it does, it burns up leaving a trail of gas and vapor in a form of 2 tails,
O True
O False

Answers

False because if it’s burnt it would leave a trail of gas but it’s said 2 tails not 1 there was only one planet

Question 4(Multiple Choice Worth 4 points)
(05.01 LC)
Which process turns glucose into energy?
O Breathing
O Cell division
O Cellular respiration
Photosynthesisch

Please help ASAP

Answers

Answer:

cellular respiration

Explanation:

it involves the chemical activities of the cell in which glucose is broken down by a series of reaction controlled by the enzymes to release energy

Answer:

The answer is C

Explanation:

The answer is C because the process of cellular respiration, the energy in food is converted into energy that can be used by the body's cells. During cellular respiration, glucose and oxygen are converted into carbon dioxide and water, and the energy is transferred to ATP.

In cellular respiration, the energy for most ATP synthesis is supplied by:


A. High energy phosphate bonds in organic molecules


B. A proton gradient across a membrane


C. Converting oxygen to atp


D. Transferring electrons from organic molecules to pyruvate


E. Generating carbon dioxide and oxygen in the electron transport chain

Answers

Answer:

B. A proton gradient across a membrane.

Explanation:

Cellular respiration can be defined as a series of metabolic reactions that typically occur in cells so as to produce energy in the form of adenosine triphosphate (ATP). During cellular respiration, high energy intermediates are created that can then be oxidized to make adenosine triphosphate (ATP). Therefore, the intermediary products are produced at the glycolysis and citric acid cycle stage.

Additionally, mitochondria provides all the energy required in the cell by transforming energy forms through series of chemical reactions; breaking down of glucose into Adenosine Triphosphate (ATP) used for providing energy for cellular activities in the body of living organisms.

Basically, oxygen goes into the body of a living organism such as plants, humans and animals when they breathe while glucose is absorbed by the body when they eat.

In cellular respiration, the energy for most ATP synthesis is supplied by proton gradient across a membrane.

This ultimately implies that, most adenosine triphosphate (ATP) is formed due to the net movement of protons against a concentration gradient in the electron transport chain.

What is this organelle?

Answers

Answer:

It's the mitochondria

Explanation:

The mitochondria is the powerhouse of the cell.

A beetle moving away from a light source is an example of
0 Taxis
0 Imprinting
O Imitation
O Kinesis

Answers

Kinesis because he is moving away from an external stimulus.

A dog pants in the heat to control its body temperature. Panting is a mechanism of?

Answers

Answer:

A dog pants in the heat to control its body temperature. Panting is a mechanism of homeostasis that dogs use for regulation .

The drug chloral hydrate prevents elongation of microtubules. During which stage of the cell cycle would chloral hydrate be most
harmful?
Multiple Choice
M phase
G1
G2
S phase
cytokinesis

Answers

Answer: G1 phase

Explanation:

Hope this helps!

Please someone help me ASAP please

Answers

Answer:

a. Translocation

Explanation:

Translocation is a type of chromosomal abnormality in which a chromosome breaks and a portion of it reattaches to a different chromosome.

Which has been justified in the picture.

Imagine that you are trapped within the exclusion zone. As you hike your way out, you begin to feel hungry. Using what you know about biomagnification, which food source would be the
safest? Explain how you know.
a. Mushrooms, fruit, and berries
b. Moose or deer meat.
C. Wolf meat.
d. Eagle meat.

Answers

Probably moose or deer meat the others captain bad stuff and some berry and mushrooms can be poisonous. We eat elk and deer meat a lot!

The food sources that would be the safest are mushrooms, fruit, and berries.

What do you mean by Biomagnification?

Biomagnification may be defined as the process by which a compound such as toxins, pollutants, etc. increases its concentration in the tissues of organisms as it travels up the food chain.

Mushrooms, fruit, and berries are present at the lowest level of the trophic level because they are autotrophs. Due to their position, they contain the lowest amount of toxins in them, and hence are more safest.

Therefore, the food sources that would be the safest are mushrooms, fruit, and berries.

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The plasma membrane is

Answers

is the membrane found in all cells that separates the interior of the cell from the outside environment.

Answer:

The plasma membrane, also called the cell membrane, is the membrane found in all cells that separates the interior of the cell from the outside environment. ... The plasma membrane consists of a lipid bilayer that is semipermeable. The plasma membrane regulates the transport of materials entering and exiting the cell.

Explanation:

Which of these is evidence of global warming?
OA. Stable precipitation patterns
O B. Falling global temperatures
OC. An increase in the size of glaciers
OD. Overall rising sea levels

Answers

D, the others are opposites

The evidence of global warming is best seen in the overall rising sea levels.

How high are the seas?

The average height of the ocean's surface above the surface of the land is referred to as sea level. It is a crucial gauge of climate change on Earth and is influenced by a number of variables, such as variations in global temperatures, ocean currents, and land movements.

This is brought on by the melting of glaciers and polar ice caps as well as the thermal expansion of warming seas. Sea levels are increasing more quickly as the earth heats, which is a direct outcome of global warming.

The other possibilities, such consistent precipitation patterns, declining global temperatures, and an expansion of glaciers in size, are not necessarily signs of global warming.

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What is the most likely inheritance pattern for red-green
colorblindness based on the pedigree?
A) autosomal dominant
B) autosomal recessive
C) sex-linked dominant
D) sex-linked recessive

Answers

Answer:

c

Explanation:

Which of the following statements stated below is precisely correct?
O A. Oxaloacetate is always regenerated during the Krebs cycle.
O B. The second substrate-level phosphorylation occurs during Krebs cycle.
O C. All the enzymes that catalyze the Krebs cycle are dehydrogenase.
O D. Answers A and B are the right answer choices for this question.
O E. Answers A, B, and C are the right answer choices for this question.

Answers

Both a b c are the right answer choice for this question

Complete the following sentence using the most appropriate words.
A blood sample obtained from the crime scene was sent to the laboratory to determine if it contained any drugs.
The[microcrystalline, color, spectroscopy]
test was performed on the sample, which involved mixing it with a reagent and observing the result using the[spectroscopy, chromatography, microscopy]
technique.

Answers

Answer:

Microcrystalline

Chromatography

Explanation:

A blood sample obtained from the crime scene was sent to the laboratory to determine if it contained any drugs.

The MICROCRYSTALLINE test was performed on the sample, which involved mixing it with a reagent and observing the result using the CHROMATOGRAPHY technique.

How does cellular respiration directly benefit animals?

Answers

because they inhale oxygen and exhale carbon dioxide.
During cellular respiration animal cells combine oxygen with food molecules to release energy to live and function. Remember that cellular respiration produces carbon dioxide as a waste product. Animals use energy to grow, reproduce, and to function. They release the carbon dioxide into the air as a waste product.

Do AIDS transfer by saliva and sweat??????​

Answers

It can only be transmitted when certain fluids from an HIV-positive person move directly into the bloodstream or through mucous membranes of an HIV-negative person. Tears, saliva, sweat, and casual skin-to-skin contact can't transmit HIV.

Describe how air pollution can lead to water pollution.

Answers

Answer:Atmospheric deposition is the pollution of water caused by air pollution. In the atmosphere, water particles mix with carbon dioxide sulphur dioxide and nitrogen oxides, this forms a weak acid. ... When acid rain pollutes marine habitats such as rivers and lakes, aquatic life is harmed.

Explanation:

Rain falls into water polluting it

PLEASE HELP ASAP!!!
What is one example of an index fossil?
1. a rock
2. a tooth
3. a trilobite
4. a meteor

Answers

The answer is a rock !!!!

Answer: #1. A Rock

Definition: A fossil that is useful for dating and correlating the strata in which it is found.

Note: Hope this helps answer is from Acceleration Education / Buzz.

All My Other Answers:

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A genetic engineer wants to isolate a gene from a scorpion that encodes the deadly toxin found in its stinger, with the ultimate purpose of transferring this gene to bacteria and producing the toxin for use as a commercial pesticide. Isolating the gene requires a DNA library. Should the genetic engineer create a genomic library or a cDNA library

Answers

Answer:

The correct answer is - Yes, the genetic engineer should create a genomic library or a cDNA library.

Explanation:

mRNA isolated from the toxin extracted from the venom gland of the scorpion to create a cDNA library. The cDNA sequence is reverse transcribed by the mRNA that lacks the introns as prokaryotic organisms such as bacteria cannot splice introns.

If the engineer wants to express the toxin in bacteria then he required a cDNA sequence as a genomic clone can not use to clone for making proteins.  mRNA should be isolated from the venom gland so it can synthesize cDNA library enriched with toxin protein sequence.

The
secretes special enzymes that help the stomach digest fats.

Answers

Answer:

The pancreas gland lies just beneath the stomach.

Explanation:

It produces a host of digestive enzymes, including lipases, which are essential in fat digestion. The pancreas also secretes bicarbonate ions, which help to neutralize the pH of the chyme entering from the stomach.

The body functions best with a particular set of conditions: an ideal body temperature, blood pressure, blood sugar level, and many other ideal conditions. Which of the following is NOT true regarding homeostasis?

Answers

Answer:

what are the answer chocices

Explanation:

Explanation:

where is the answer choice??

How do simple and complex sugars compare in their effect on your glycemic index?

Answers

Answer:

The glycemic index allows you to compare the ability of foods to increase blood sugar (glucose) levels. In general, simple carbohydrates have a medium to high glycemic index. In the case of complex carbohydrates, this varies.

Explanation:

The Glycemic Index (GI) is a measure that provides information on how quickly blood sugar levels rise after eating a certain food. Low glycemic index foods are those that have a low content of sugars or simple carbohydrates and that help regulate blood glucose levels, the consumption of foods with a low glycemic index has been proposed as a prevention and / or management factor of obesity, cardiovascular diseases, type 2 diabetes mellitus and some types of cancer. Complex carbohydrates are made up of larger, more complex molecules and are found primarily in the form of starch and fiber. Foods high in fat or fiber are digested more slowly, so they also cause a slower rise in blood sugar. A food with a high GI raises blood sugar faster than foods with a medium or low GI. The GI is higher when foods do not contain fiber and they are easily digested, since they reach the bloodstream quickly, it is also higher if there is sugar or if cooking destroys part of the fiber and favors the digestion of food.

Living organisms release carbon dioxide as a product of photosynthesis
a. True
b.false

Answers

a. true

Explanation:

hope it will helpful to you

If you are talking about plants it is false because they release oxygen as a “waste” product from photosynthesis but if the question is referring to animals and human beings the answer would also be false because we do not undergo photosynthesis. Best bet is false

What purpose of transportation? Where does it happen the cell

Answers

Answer:

Explanation: How do things move across a cell membrane?

Summary. Controlling the movement of things in and out of the cell is an important role of the plasma membrane. ... Osmosis is the diffusion of water molecules across a membrane. Water moves in or out of a cell by osmosis until its concentration is the same on both sides of the plasma membrane.

Materials move within the cell's cytosol by diffusion, and certain materials move through the plasma membrane by diffusion (Figure 3). Diffusion expends no energy. On the contrary, concentration gradients are a form of potential energy, dissipated as the gradient is eliminated
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