3.We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non- functional protein from the method used and results obtained this semester

Answers

Answer 1

Answer:

In the given case, the protein would have required to go through the process of post-translational modification, however, the mentioned protein would have been cultured in the prokaryotic system, the process of post-translational modification would have failed to take place. This step would be essential for the functionality of the protein. Thus, there is a need to culture it within the eukaryotic system.

The covalent and generally the enzymatic modification of proteins post its biosynthesis is termed as post-translational modification.


Related Questions

What is a stimulus?
there are no options actually so plz help thank you

Answers

Answer:

In general, a stimulus is something that provokes or causes an action or response, as in Failing that test was the stimulus I needed to start studying harder. The plural of stimulus is stimuli. Its verb form is stimulate, which typically means to spur into action or to invigorate.

Explanation:

Answer:

In physiology, a stimulus is a detectable change in the physical or chemical structure of an organism's internal or external environment. The ability of an organism or organ to detect external stimuli, so that an appropriate reaction can be made, is called sensitivity

Explanation:

it just is

which of the following best describes how the potential is affected by the structural change in the gene?

Answers

Answer:

A gene mutation is a permanent alteration in the DNA sequence that makes up a gene, such that the sequence differs from what is found in most people. Mutations range in size; they can affect anywhere from a single DNA building block (base pair) to a large segment of a chromosome that includes multiple genes.

Explanation: did the test and got it right

If any of y’all can give me the definition for these words I will give 5 stars ,like and brainlest or whatever it’s called

Answers

Answer:

rRNA - Ribosomal RNA, a molecular component of a ribosome, the cell's essential protein factory. Strictly speaking, ribosomal RNA (rRNA) does not make proteins. It makes polypeptides (assemblies of amino acids) that go to make up proteins

tRNA - Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps decode a messenger RNA (mRNA) sequence into a protein. tRNAs function at specific sites in the ribosome during translation, which is a process that synthesizes a protein from an mRNA molecule.

Explanation:

dy÷dx=(x-1)(x+3) at x=2​

Answers

Answer:

[tex]\dfrac{dy}{dx}=\dfrac{4}{25}[/tex]

Explanation:

The given expression is :

[tex]y=\dfrac{(x-1)}{(x+3)}[/tex]

We need to find dy/dx at x = 2

[tex]\dfrac{dy}{dx}=\dfrac{d}{dx}(\dfrac{x-1}{x+3})\\\\=\dfrac{(x+3)\dfrac{d}{dx}(x-1)-(x-1)\dfrac{d}{dx}(x+3)}{(x+3)^2}\\\\=\dfrac{x+3-(x-1)}{(x+3)^2}\\\\=\dfrac{x+3-x+1}{(x+3)^2}\\\\\dfrac{dy}{dx}=\dfrac{4}{(x+3)^2}[/tex]

Put x = 2 in above expression

[tex]\dfrac{dy}{dx}|x=2=\dfrac{4}{(2+3)^2}\\\\=\dfrac{4}{25}[/tex]

Hence, the value at dy/dx is [tex]\dfrac{4}{25}[/tex]

Pleaseeee help!!! (psychology edge) Androgens and estrogens are hormones that impact the sexual response cycle. Write about the four phases of that cycle and explain how the two types of hormones are involved.

Answers

Answer:

Females and males each have some amount of the sex hormones, androgens and estrogens. In males however, androgens like testosterone is more dominant and in females estrogens like estradiol are more dominant. The hormones both affect the sexual response cycle. The sexual response cycle has four phases: desire, arousal, orgasm and resolution. The desire phase can last anywhere from a few minutes to hours and includes physical changes like an increase in muscle tension, heartbeats quickening, flushed skin, and an increased flow of blood to genitals. The arousal phase extends to the brink of the orgasm phase, during this phase the changes experienced in the desire phase are intensified along with blood pressure increasing. The orgasm phase generally only lasts a few seconds and is the climax of the sexual response cycle, some characteristics include: Involuntary muscle contractions and spasms, blood pressure, breathing, and heart rate at their highest point, a release of built up sexual tension, and flushed skin. During the resolution phase the body returns to normal, often a sense of wellbeing and fatigue is achieved. To begin the cycle a male need a certain amount of the androgen, testosterone, but high levels in females tend to lead to more sexual thought and desires, typically though, women have relatively low levels of testosterone. In women, estrogens, such as estradiol are more common. Estrogens are produced when a girl starts puberty and helps her reproductive cycle begin. A high level of estrogen in men can lead to a reduced sex drive, loss of hair, or trouble focusing, but a normal level in men is crucial to proper erectile function.

Explanation:

I just submitted this, i hope it helps :)

Which provide evidence for the general theory of relativity? Check all that apply
gravitational lensing
the constancy of the speed of light
the curvature of space-time
changes in Mercury's orbital path
O gravitational redshift

Answers

Answer:

Gravitational redshift

Explanation:

Gravitational redshift, changes in a planet's orbital path, gravitational lensing provides concrete evidence for the general theory of relativity. Hope this is helpful and the answer you were looking for!

Answer:

1 4 5

Explanation:

Scenario 1
When you think of the American Southwest, you probably think of cacti, both big and small. If you were to
visit the American Southwest today, you might be disappointed with the lack of variation among cactus
species, especially right off the major highways. In 1926, Route 66 was the nation's first major highway to
allow visitors to visit the Southwest. Like many tourists, these visitors wanted to take home a memento of
their travels. Many people would pull over and begin looking for their own living souvenir. Since many of the
tourists did not have the necessary tools to extract a spiny cactus out of the soil, they usually had to make a
conscious decision about what type of cactus they should dig up. The cacti with too many spines were seen
as too difficult to dig out, and the visitors likely didn't have gloves to protect their hands. The cacti with too
few spines were seen as homely and were often overlooked. Therefore, the tourists found a cactus that was
between these two extremes. Over the last century, the phenotypic variation within these small cacti has
changed (at least in areas accessible by these highways).

Answers

gurl what are you asking

The unit states that any animal that can be domesticated probably already has been. Explain the logic behind the statement and whether or not you find it convincing.

Answers

Answer:

Saying that if an animal can be domesticated that it probably already has been is saying that a lot of things are documented and if it has the capability to be domesticated or brought under human control then someone somewhere probably has. So I would personally find this convincing.

Explanation:

Hope that this helps and if so please mark me the brainliest. Peace and Love

List 2 natural hazards
due to Earth's internal
convection.

Answers

Any of these should work : Volcanic Eruptions, Earthquakes, Tsunamis, Landslides. ( I would recommend going for the first two).

Please help me I do not know how

Answers

Liquid 1 and 4, because they have the same melting point and density.

One codon contains the instructions to make
- 1 protein
- 1 amino acid
- RNA

Answers

Answer:

1 protein

Explanation:

For what reasons could a theory be changed or replaced? Check all that apply.

A new, untested hypothesis is proposed.
New information is discovered that does not support the theory.
A single experiment produces results that differ from all previous experiments.
New technology is developed that provides more information about the theory.
New experimental methods are designed to test a theory.

Answers

1. New technology is developed that provides more information about the theory.

2. New experimental methods are designed to test a theory.

3. A single experiment produces results that differ from all previous experiments.

im not sure about #3 but it could be it

Answer:

A. New technology is developed that provides more information about the theory.

B. New experimental methods are designed to test a theory.

D. New information is discovered that does not support the theory.

Explanation:

just did it

Which microscope is used to check organelles of human cheek cell?

Answers

Answer:

4x or 10x

Explanation:

You need one of those to find the cells and then you can increase magnification

What are the properties of water that you can observe when transpiration takes place?

Answers

Answer:

When water evaporates through the leaves, a pull is created through the xylem, and water moves back to the leaves. This is known as the transpiration pull.

access the capabilities of animal and plant cell types owing the presence or absence of chloroplast and cell wall

Answers

Answer:At this point, it should be clear that eukaryotic cells have a more complex structure than do prokaryotic cells. Organelles allow for various functions to occur in the cell at the same time. Despite their fundamental similarities, there are some striking differences between animal and plant cells (see Figure 1).

Animal cells have centrosomes (or a pair of centrioles), and lysosomes, whereas plant cells do not. Plant cells have a cell wall, chloroplasts, plasmodesmata, and plastids used for storage, and a large central vacuole, whereas animal cells do not.

Explanation: pls give me brainest

question 1. The construction of the Temple took seven years.
False True? question 2
Solomon sinned by allowing the corruption of God's people in the worship of idols.

True
False

Answers

1.) True .... 1 Kings 9:10 says it took Solomon 20 years altogether to build the Temple and his royal palace; the Temple itself was completed in 7 years

2.) True .... God hated it he would never have allowed it ....

Answer:

Question 1 true

question 2 true

Explanation:

Type the word that appropriately completes each statement.
All fish reproduce sexually, but fertilization may occur in one of two ways.
In__________
fertilization, the female fish releases eggs in the water or on a surface. The male fish spreads
sperm over the eggs to fertilize them.
In__________
fertilization, the eggs are fertilized inside the female's body. The young develop there until the
female gives birth.

Answers

exExplanation: external internal

Hello, 3Coli here!

Here is your answer:

In  external fertilization, the female fish releases eggs in the water or on a surface. The male fish spreads sperm over the eggs to fertilize them.

In internal fertilization, the eggs are fertilized inside the female's body. The young develop there until the female gives birth.

Hopefully, this helps! :D

Ask your question below!

The correct sequence for the general adaptation syndrome is:_________
a) Exhaustion phase, alarm phase and resistance phase
b) Alarm phase, resistance phase and exhaustion phase
c) Resistance phase, exhaustion phase and alarm phase
d) Alarm phase, exhaustion phase and resistance phase

Answers

Answer:

The correct sequence for the general adaptation syndrome is: b) Alarm phase, resistance phase and exhaustion phase

Explanation:

The general adaptation syndrome describes what happens to our body when it is under stress.

The first phase is the Alarm phase. In this stage, the body activates the fight or flight response, causing an increase in adrenaline and cortisol, blood pressure, respiratory frequency, amongst other things that prepare us to react in a situation that we perceive as dangerous.

The second phase is the resistance one. During this phase, the body undergoes a different process that adapts it to manage the stress, with an elevated amount of cortisol and blood pressure.

The third phase is the exhaustion phase. If the stress levels continue to be high, the body passes to this stage. The body is exhausted and cannot keep dealing with stress, which causes mental and physical problems such as anxiety, depression, fatigue, stress-related illnesses, among other symptoms.

in chemical reactions, bonds blank in reactants.

Answers

in chemical reactions, bonds break in reactants

In a food chain or web, is organism would be classified as a(n)

Answers

Answer:

producer

Explanation:

The answer to this question would be PRODUCER . This question has already been asked on brainly

https://brainly.com/question/9870791

1. Chromosomes are made of
and​

Answers

Answer:

protein and DNA

Explanation:

protein and DNA! Hope I helped !

Food-borne illnesses can have substantial health and economic consequences. Correctly identify the steps that can lead to food-borne illness by Salmonella/Campylobacter and E. coli 0157:H7
1. Bloody diarrhea and hemolytic uremic syndrome can result
2. Live E. coli 0157:H7 bacteria are ingested
3. A Shiga-like toxin is produced
4. Incomplete cooking of raw hamburger fails to kill all pathogens
5. Live Salmonella or Campylobacter bacteria are ingested
6. Diarrhea, abdominal pain and nausea can result.
7. Incomplete cooking of poultry and fruits/vegetables fails to kill all pathogens
8. The pathogen multiplies in the intestinal tract for 2-48 days

Answers

Answer:

4,6,5,1,3

Explanation:

What is an essential characteristic of members of a given species?
the ability to produce eggs or live offspring without sexual reproduction
the ability to reproduce with members of another species
the ability to reproduce with one another and have fertile offspring
the ability to reproduce with organisms outside of their local gene pool

Answers

Answer:

the ability to reproduce with one another and have fertile offspring.

Explanation:

An essential characteristic of the members of a given species is the ability to reproduce with one another and have fertile offspring. Thus, the correct option is C.

What are the characteristics of a species?

A species is a group of organisms which are living in an area with similar characteristics which are capable of exchanging genes and chromosomes and produce new organism similar to itself. The species are important as it maintains the equilibrium in the ecosystem.

A species can be defined as the largest group of organisms with the two individuals of the appropriate sexes can produce a fertile offspring, typically through sexual reproduction. The species can also be defined on the basis of their karyotype, DNA sequence, morphology, behavior or ecological niche.

Therefore, the correct option is C.

Learn more about Species here:

https://brainly.com/question/13258090

#SPJ2

One codon has the instructions to make

Answers

Answer:

1 protein

Explanation:

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5. When it comes to punishing Antigone, what does not matter to Creon?1)O That Antigone is his family2)That Antigone is Polyneices and Eteocles's sister3)O That Antigone is betrothed to Haemon4)That Antigone is a woman5)O All of the above Drag each tile to the correct box. Find the value of x. Everything is in the photo. Which is the most accurate generalization that can be made about thispassage?A)Sojourner Truth believes that women should haveequal rights to men.B)Sojourner Truth believes that she is a woman equalto all other women.C)Sojourner Truth does not want to upset anyone withher personal beliefs.D)Sojourner Truth lists all the ways that she's beenmistreated and abused. Hector runs laps around the track. The table shows how long it takes him to run different numbers of laps. How long would it take Hector to run 7 laps?A.35 minutesB.25 minutesC. 15 minutesD. 45 minutes why is Canada separated from the U.S? Help appreciated, thanks so much! Unit 7 homework 1: Gina Wilson answer keyDoes any one have the anwerkey? Larry will probably be a late bloomer socially, just like his older brothers the questions on a multiple-choice test each have 4 answer choices. Describe a model that you could use to simulate the outcome of guessing the correct answer to a 100 questioned test Which equation represents a graph with a vertex at (1, -6)?A. y=3x2+6x3B. y=3x2-6x-3C. y=3x2-8x-1D. y=3x2-3x-6 Think of a successful business and research the founding of that business. What successes could you learn about from that business? Where did the 45% come from. It states 55% in question PLEASE HELP I HAVE A TIME LIMIT I WILL GIVE BRAINLIEST AND 5 STARS show your work rex mixed x liters of fruit syrup with y liters of water to make fruit punch three times the number of liters of water he used is equal to 6 more than twice the number of liters of fruit syrup he used the total number of liters of syrup and water he mixed was 7 liters what is the number of liters of fruit syrup and water that rex added to make the fruit punch Aaron spent $35 to buy some new baseballcards. Each pack of cards cost the same wholedollar amount. How many packs of cardscould he have bought? Write a title for a magazine in which you might find the article which accompanies this visual Ankur estimated the quotient of 15 and one-third divided by (negative 4 and two-thirds) to be 3. Which best describes his error?Ankur multiplied the compatible numbers 15 and 3.Ankur found that the quotient of a positive number and a negative number is negative.Ankur found that the quotient of a positive number and a negative number is positive.Ankur added the compatible numbers 15 and 3. 16 is what percent of 20? How did nationalism lead to WWI? dydx=(x-1)(x+3) at x=2