2. they describe conjugation (matings) done in liquid media where the recipient cells used to be on a biofilm and compared them with matings done with cells still on a biofilm. why did they do these by, at least, triplicate?

Answers

Answer 1

Conjugation in liquid media was performed in triplicate to ensure a consistent and reliable result. By performing the experiment multiple times, it allows for the possibility to compare and contrast the effects of the mating in different scenarios.

Furthermore, it provides a better understanding of the behavior of the recipient cells when they are no longer in the biofilm. For instance, the researchers may be able to measure the relative fitness of the cells when they are in liquid media compared to when they are still in the biofilm.

Additionally, replicating the experiment would reduce any variability in the results, ensuring that the conclusions drawn are reliable.  

In summary, performing the conjugation experiment in triplicate allows for a more comprehensive evaluation of the effects of the mating, as well as provide a better understanding of the behavior of the recipient cells in different environments.

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Related Questions

when jeremy smith was in the shower, the hot water ran out. the cold water caused the hairs on his skin to stand up. this body response to cold is known as . group of answer choices exfoliation anhidrosis piloerection perspiration

Answers

Jeremy Smith's body response when the cold water caused the hairs on his skin to stand up is known as piloerection.

Thus, the correct answer is piloerection (D).

Piloerection in humаns is аn аutonomic response observed during а vаriety of strong emotionаl experiences, including feаr аnd аnger, аesthetic pleаsure, аwe, аnd surprise.

Piloerection, also known as goosebumps or goose pimples, is the erection of the hаir of the skin due to contrаction of the tiny аrrectores pilorum muscles thаt elevаte the hаir follicles аbove the rest of the skin аnd move the hаir verticаlly, so the hаir seems to 'stаnd on end.'

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the red portion of the human lip: question 12 options: integumentary lip. has no facial markings. must be treated by hypodermic tissue building in every case. mucous membrane.

Answers

The red portion of the human lip is known as the mucous membrane. It does not have any facial markings and must be treated by hypodermic tissue building in every case.

What is the mucous membrane?

The mucous membrane is a layer of tissue that lines various parts of the body's openings and cavities that are in contact with the outside environment. It is a moist membrane that secretes mucus, a slimy substance that assists in trapping germs and other foreign substances, as well as keeping the surface moist.

The red portion of the human lip: Mucous membrane. The red portion of the human lip is the mucous membrane. The mucous membrane of the lips is often known as the vermilion zone. It is a transition zone between the skin and the mucous membrane.

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meiosis divides one cell into four cells, but the resulting cells have half the amount of dna as compared to the original cell. how do you think this is possible?

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During meiosis, one cell is divided into four cells, but the resulting cells have half the amount of DNA as compared to the original cell. This is because of the two cell divisions, meiosis I and meiosis II, that occur during meiosis.

During meiosis I, homologous chromosomes separate, resulting in two cells with half the number of chromosomes as the original cell.

During meiosis II, sister chromatids separate, resulting in four cells, each with half the number of chromosomes as the original cell.

In other words, the resulting cells have half the amount of DNA because meiosis results in four cells, each containing half the number of chromosomes and, therefore, half the amount of DNA as the original cell.

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Help with my biology please

Answers

Carbohydrates are composed of monosaccharides, proteins are composed of amino acids, and nucleic acids are composed of nucleotides.

What are the elements present and the building blocks in carbohydrates, proteins, and nucleic acids?

Carbohydrates, proteins, and nucleic acids are three major classes of biomolecules that are essential for life.

Here are the elements present and the building blocks of each:

Carbohydrates:

Carbohydrates are organic molecules that contain carbon, hydrogen, and oxygen in the ratio of 1:2:1. The building blocks of carbohydrates are monosaccharides, which are simple sugars that cannot be broken down into smaller molecules. Examples of monosaccharides include glucose, fructose, and galactose.

Proteins:

Proteins are complex molecules that are made up of amino acids. Amino acids contain carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur. There are 20 different types of amino acids, and they are joined together by peptide bonds to form polypeptide chains, which fold into specific three-dimensional structures to form proteins.

Nucleic acids:

Nucleic acids are macromolecules that store and transmit genetic information. They are composed of nucleotides, which are made up of a nitrogenous base, a sugar, and a phosphate group. The four nitrogenous bases in DNA are adenine, guanine, cytosine, and thymine, while in RNA, uracil replaces thymine. The sugar in DNA is deoxyribose, while in RNA, it is ribose. The nucleotides are joined together by phosphodiester bonds to form a linear chain called a polynucleotide.

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. gap junctions are important in smooth muscle. when absent, as in the iris of the eye, what new characteristic does the smooth muscle fibers take on?

Answers

Gap junctions are important in smooth muscle. When they are absent, as in the iris of the eye, the smooth muscle fibers will exhibit an independent behavior.

What are gap junctions?

Gap junctions are protein channels that connect the cytoplasm of two adjacent cells. In animals, gap junctions are found in almost every tissue and are particularly abundant in muscle and nervous tissue. Gap junctions are made up of protein subunits that connect adjacent cells, allowing molecules and ions to flow freely between them, creating a direct link between them. Gap junctions have been linked to various physiological processes, including regulation of cell growth and differentiation, metabolism, and electrical and chemical signaling.

How are smooth muscle fibers affected when gap junctions are absent?

Smooth muscle fibers are affected when gap junctions are absent in a number of ways. Gap junctions are important in smooth muscle because they allow adjacent cells to contract in synchrony. When the gap junctions are absent, smooth muscle fibers exhibit independent behavior. The absence of gap junctions causes smooth muscle fibers to be more excitable, allowing them to contract in response to different stimuli. The fibers also tend to have a longer duration of contraction, which can lead to prolonged muscle tone or spasm.

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Blood has to get to the lungs to unload carbon dioxide. Logically, what should happen when this waste builds up in the blood?
a. slow the movement of blood through the body
b. speed through the movement of blood through the body
c. not change the movement

Answers

Answer: b

Explanation:the CO2 is waste product created by metabolic

when considering the transfer and capture of potential energy derived from glucose during cellular respiration, which molecule carries the smallest amount of that potential energy?

Answers

When considering the transfer and capture of potential energy derived from glucose during cellular respiration, the molecule that carries the smallest amount of that potential energy is ATP. The potential energy that is derived from glucose during cellular respiration is converted into ATP.

ATP (adenosine triphosphate) is an important organic molecule that plays a major role in the cells. It carries energy that is required by cells for several processes. It is formed by the cells in a process known as cellular respiration.

Cellular respiration occurs in three stages: glycolysis, the citric acid cycle (Krebs cycle), and electron transport chain. The main function of cellular respiration is to break down the glucose to produce ATP molecules.

During cellular respiration, the energy that is stored in glucose is released and captured in the form of ATP.ATP is an unstable molecule that contains a large amount of potential energy. It carries energy in the form of chemical bonds. When these bonds are broken, energy is released. The energy that is released from ATP is used by cells for several processes such as muscle contraction, active transport, and many other functions. Therefore, ATP is a molecule that carries the smallest amount of potential energy.

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Which of the following is NOT found in saliva? A) urea and uric acid. B) electrolytes. C) lysozyme. D) protease. D) protease.

Answers

Proteases enzyme is not found in saliva , hence option 'D' is correct

The natural execration occurs from salivary gland, thus it accounts for high concentration of urea and uric acid found in saliva. Since the amount of creatinine production is consonant in 24 hours , uric acid and urea -to- creatinine ratio are better to clarify the changes of this compound concentration in saliva . Therefore option A is incorrect.

The main inorganic components are sodium , potassium, chloride, calcium, phosphate , and bicarbonate , all contributing to the ionic strength of saliva. Therefore option B is incorrect.

As an important part of the non specific immune defense mechanism , lysozyme is an important component of antibacterial in saliva. Therefore option C is incorrect.

Proteases are released by pancreas into the proximal small intestine ,where the mix with proteins already denatured by gastric secretion's and break down into amino acids. Therefore option "D" is correct.

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what enzymes perform most of their work on the lagging strand, but very little on the leading strand? g

Answers

The enzymes primarily responsible for the replication of the lagging strand during DNA synthesis are DNA polymerase and DNA primase.

DNA polymerase is the enzyme that synthesizes new strands of DNA by adding nucleotides one by one in the 5' to 3' direction. DNA primase synthesizes short stretches of RNA primers, which act as starting points for DNA polymerase to bind and begin synthesis.
On the lagging strand, DNA polymerase must move backwards in order for synthesis to occur, creating an RNA primer with DNA primase at the beginning of each Okazaki fragment. DNA polymerase can then bind to this primer and begin synthesis.

Very little of this occurs on the leading strand, since DNA polymerase can move in the same direction as the leading strand.

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is any energy released during atp hydrolysis and if so, how much? multiple choice question. energy is required for atp hydrolysis. a considerable amount of energy is released. a very small amount of energy is released.

Answers

A considerable amount of energy is released during ATP hydrolysis. The amount of energy that is released during ATP hydrolysis is about 30.6 kJ/mol (or 7.3 kcal/mol).

The process of ATP hydrolysis is known to be very important in the biochemical reactions taking place in living organisms. When ATP is hydrolyzed, it is converted to ADP and phosphate, releasing energy. ATP hydrolysis is often coupled with biochemical reactions, such as muscle contraction, protein synthesis, and active transport, which require energy. The amount of energy that is released during ATP hydrolysis is about 30.6 kJ/mol (or 7.3 kcal/mol). The energy released during ATP hydrolysis is primarily used to drive endergonic reactions in the cell.

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imagine a condition where the vessels that carry blood between the lungs and the body tissues were permeable to oxygen. what would you expect to observe relative to the normal condition of low permeability to oxygen in the vessels that carry blood from the lungs to the tissues?

Answers

If the vessels between the lungs and body tissues were permeable to oxygen, there will be a decrease in the oxygen supply to the body tissues.

Normally, oxygen-poor blood from the body tissues flows into the right side of the heart, and is then pumped to the lungs where it picks up oxygen and releases carbon dioxide. The oxygen-rich blood then flows back to the left side of the heart, where it is pumped out to the body tissues to supply oxygen to the cells.

If the vessels between the lungs and body tissues were permeable to oxygen, oxygen-rich blood from the lungs would flow into the right side of the heart, mix with oxygen-poor blood from the body tissues, and then be pumped out to the body tissues.

This would result in a reduced delivery of oxygen to the tissues, as some of the oxygen-rich blood from the lungs would bypass the body tissues and flow back to the lungs.

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Analysis Questions. To answer questions #1-5, use the letter "f" to indicate the recessive Falcon anemia allele
and the letter "F" for the normal allele. [5pts]
1. What is Arlene's genotype? Response

Answers

Answer:

Explanation:

You must include the phenotype of Arlene so we can determine the genotype. Without any information, I am unable to help you.

If you can provide me with Arlene's phenotype or any other information, I will be glad to help.

Just comment below!

what is the relationship between respiration and activity

Answers

Respiration and activity are closely related in the sense that respiration is the process by which our bodies generate energy to fuel physical activity.

What happens during respiration?

During respiration, our bodies break down glucose and other molecules using oxygen to produce ATP, which is the primary energy currency of the body. This ATP is then used by the muscles to perform physical activity such as walking, running, lifting weights, or any other form of movement.

The rate of respiration increases as the body's demand for energy increases during physical activity. This increased respiration rate helps to supply more oxygen to the muscles, which allows them to continue functioning and producing ATP to support the activity.

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How long will most likely take before Isla education is finished and she is ready to begin working? Oh she just graduated veterinary school.
A. It should be within the week because the program is a weekend one
B. They can plan for next month as the training only takes a few weeks
C. It will likely be several months before Isla is ready to work.
D. Taking on this course means that Isla has at least three more years of school

Answers

Answer:

C. It should be within the week because the program is a weekend one

in hemoglobin mckees rocks, point mutation occurs at the codon for tyrosine (uau) to stop codon uaa. what kind of point mutation is this?

Answers

In hemoglobin mckees rocks, point mutation occurs at the codon for tyrosine (uau) to stop codon uaa. This is an example of a nonsense mutation.

A nonsense mutation is a point mutation that results in the formation of a stop codon, causing a premature end to the mRNA strand. In this example, the codon for tyrosine (UAU) is changed to a stop codon (UAA). This mutation causes the truncation of the mRNA strand, which results in the production of an incomplete and often non-functional protein.
It can have severe consequences, depending on where in the gene sequence they occur. In some cases, the mutated gene may still produce some functional protein, but not at the expected levels. In other cases, the mutated gene may produce no functional protein at all. Furthermore, a nonsense mutation may cause a gene to produce a truncated protein that is harmful to the organism.

Overall, nonsense mutations are a type of point mutation that results in a premature termination of the mRNA strand. This can have a variety of consequences, depending on where in the gene sequence the mutation occurs and the type of protein being produced.

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Is Lightning striking the ocean a chemical or physical change?

Answers

Answer: chemical change

Explanation:

which activity might reduce habitat loss?(1 point) responses draining a wetland to plant fruit trees draining a wetland to plant fruit trees spreading out the human population spreading out the human population replacing golf courses with farms replacing golf courses with farms removing invasive species

Answers

The activity that might reduce habitat loss is removing invasive species.

Removing invasive species can help to restore native habitats and reduce the negative impacts of invasions on biodiversity. By reducing the competitive pressure from invasive species, native species may be better able to thrive and reproduce, which can ultimately lead to the restoration of habitat.

On the other hand, activities such as draining wetlands or replacing golf courses with farms can actually contribute to habitat loss, as they involve the destruction or alteration of natural habitats.

Spreading out the human population may help to reduce habitat loss by reducing the pressure on natural areas, but it may not be a practical or feasible solution in many cases.

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How does a small population
cause evolution to occur more
quickly than in a large
population?
A. A small population is more likely to have
mutations.
B. A small population is affected more by a
change in allele frequency than a large
population.
C. The small populations have more variations
than large populations.
D. Small populations tend to have a higher
young population than large populations.

Answers

Answer:

Explanation:

B is the answer it Allele means DNA or genes .

decide which of the highlighted substituents has the higher priority. molecule higher priority? molecule higher priority? a b a b a b a b

Answers

The substituent in molecule a has higher priority as it has a higher atomic number than the substituent in molecule b. Hence, the correct answer is 'a' has the higher priority.

When comparing the priority of the highlighted substituents in the given molecules, we need to use the Cahn-Ingold-Prelog priority rules. These rules are used to determine the priority of substituents based on their atomic number or atomic mass.

Let's compare the highlighted substituents in each molecule: Molecule

a: The highlighted substituent in molecule a contains a bromine atom (Br), which has an atomic number of 35.Molecule b: The highlighted substituent in molecule b contains a chlorine atom (Cl), which has an atomic number of 17.

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6. What type of body blan do sponges have?
A. Cephalization
B.asymmetry
C.bilateral symmetry
D.radial symmetry

Answers

The Option B is correct. The body blan  have the assymetry types of sponges in them

What are sponges?

Sponges (Phylum Porifera) are a type of aquatic animal that lack true tissues and organs. They are considered the simplest of all animals and do not have a body plan based on symmetry.

Instead, sponges exhibit a type of symmetry known as "asymmetry," which means they have no plane of symmetry or any organization of body parts around a central axis. Therefore, the correct answer is B. asymmetry.

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which of the following accurately describes a mechanism by which spontaneous heritable mutations can appear?

Answers

Spontaneous heritable mutations can arise due to a variety of mechanisms. These include the mutation of existing DNA, the insertion of DNA from another organism, or the incorporation of mobile genetic elements such as transposons.

Mutations can also occur due to errors in DNA replication or through damage to DNA caused by environmental agents such as radiation and chemicals. All of these processes can lead to changes in gene sequence and can result in the appearance of heritable genetic changes in the organism.

Mutation of existing DNA occurs when changes are made to the sequence of an existing gene. These mutations can result in changes in the gene's structure or function and can be caused by a variety of factors such as errors in replication or chemical or radiation damage.

Insertion of DNA from another organism can occur through horizontal gene transfer, a process by which genetic material from one organism is transferred to another organism. This process can result in the insertion of new genetic material into the organism, which can lead to the appearance of new traits and the emergence of new species.

Incorporation of mobile genetic elements such as transposons can also result in heritable genetic changes. Transposons are pieces of DNA that can move between different locations within a genome and can result in the insertion of new genetic material into a genome. This process can lead to changes in gene expression and can result in the emergence of new traits in an organism.

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which of the following is not part of bergmann's rule? group of answer choices longer limb lengths are predicted in hot climates larger body is predicted in cold climates smaller body mass is predicted in hot climates b and c only none of the above

Answers

Bergmann's rule is a biogeographic rule that states that warm-blooded animals living in colder climates will typically be larger in size than those living in warmer climates. The correct answer is option C, which is smaller body mass is predicted in hot climates.

According to Bergmann's rule, a larger body size is predicted in colder climates. This is because larger animals have a smaller surface area to volume ratio, which helps them retain heat more effectively in cold environments. Smaller animals have a larger surface area to volume ratio, which makes it harder for them to retain heat in cold environments. However, Bergmann's rule does not predict smaller body size in hot climates. Instead, it predicts longer limb lengths in hot climates. This is because longer limbs have a larger surface area to volume ratio, which helps animals dissipate heat more effectively in hot environments. Therefore, option C is the correct answer. Bergmann's rule is one of several biogeographic rules that describe patterns in the distribution and evolution of animals around the world. These rules can be useful in understanding how animals adapt to their environment and how they may respond to climate change.

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how does binding of complement-opsonized microbes to cr1 facilitate clearing of the microbe from the host?

Answers

The complement-opsonized microbe binds to CR1 receptors located on phagocytic cells, such as macrophages and neutrophils. This binding triggers the phagocyte to engulf the microbe and remove it from the host. The binding also helps the phagocyte to recognize the microbe, which can be beneficial in the case of microbes which do not cause damage to the host.

The binding of complement-opsonized microbe to CR1 also activates the complement cascade, which helps to remove the microbe more quickly by opsonizing additional targets and by recruiting more immune cells.

In addition, binding of the microbe to CR1 triggers release of cytokines and chemokines, which attract additional immune cells to the site of infection and activate them. This increases the chances of clearing the microbe from the host.

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transport of a solute across a membrane where the solute is going up its concentration gradient and using protein carriers driven by the expenditure of chemical energy, is known as

Answers

Transport of a solute across a membrane where the solute is going up its concentration gradient and using protein carriers driven by the expenditure of chemical energy is known as active transport.

What is active transport?

Active transport is the movement of molecules against the concentration gradient, which means moving from lower to higher concentrations. It involves a direct energy source (ATP) to drive the movement of molecules. The active transport method involves the use of protein pumps to move molecules across the cell membrane. These pumps can help move molecules, including sodium, calcium, and potassium, against the concentration gradient, which allows the cell to regulate what enters and exits. During active transport, the cell must use energy in the form of ATP to transport the molecules.

In summary, the transport of a solute across a membrane, where the solute is going up its concentration gradient and using protein carriers driven by the expenditure of chemical energy, is known as active transport. Active transport requires energy, which is provided by the hydrolysis of ATP. Active transport is necessary because it allows the cell to maintain its internal environment despite the external environment's changes.

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what type of motor neurons ensure that the spindle continues to provide information about muscle length during muscle contraction?

Answers

Answer: Gamma motor neurons

Explanation:

Gamma motor neurons innervate the intrafusal muscle fibers to set the sensory sensitivity to static and dynamic changes in muscle length.

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which of the following are consequences of hyperventilation? a. increasing h levels b. increasing alveolar pco2 c. decreasing arterial pco2 d. higher blood ph

Answers

The consequences of hyperventilation include increasing H+ levels, increasing alveolar PCO₂, decreasing arterial PCO₂, and higher blood pH. So All option is true.

Hyperventilation is a condition in which an individual breathes in more oxygen and exhales more carbon dioxide than is necessary for normal breathing. The consequences of hyperventilation include:

Increasing H⁺ levels: Hyperventilation causes the body to become overly alkaline, which increases H+ levels in the blood.Increasing alveolar PCO₂: The increased breathing leads to an increase in the partial pressure of carbon dioxide in the alveoli of the lungs.Decreasing arterial PCO₂: Hyperventilation causes the partial pressure of carbon dioxide in the arteries to decrease.Higher blood pH: The increase in H⁺ levels and the decrease in arterial PCO₂ results in an increase in the blood pH.

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what was the control group in this study? a the transplanted population in the killifish pools b the transplanted population in the pike-cichlid pools c the source population in the killifish pools d the source population in the pike-cichlid pools

Answers

In an ecological study involving killifish and pike-cichlid pools, the control group is the source population in the pike-cichlid pools as it did not receive any intervention in the study.

In a study, the control group refers to the group that does not receive any treatment or intervention and is used as a comparison to the experimental group. In this scenario, the source population in the pike-cichlid pools is the control group as it did not receive any intervention in the study. The study is not mentioned in the question, but based on the options provided, it is likely an ecological study involving killifish and pike-cichlid pools. The transplanted population is most likely the experimental group. The source population in the killifish pools and the source population in the pike-cichlid pools are both control groups that did not receive any intervention in the study.

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FILL IN THE BLANK. when setting up to make a gta weld on low carbon steel, the tungsten should be __ and the welding machine set for ___.

Answers

When setting up to make a GTA weld on low carbon steel, the tungsten should be sharpened to a point and the welding machine set for alternating current (AC).

GTA welding, also known as gas tungsten arc welding (GTAW), is a welding technique that utilizes an arc between a non-consumable tungsten electrode and the workpiece to generate heat. The weld pool is formed by the heat generated from the arc melting the base metal and the filler metal (if used). The weld area is protected from atmospheric gases by an inert shielding gas, usually argon or helium, which is supplied from a bottle or external source.

The tungsten is sharpened to a point because it will be used as an electrode, which will serve as the arc's cathode. The sharp point of the tungsten electrode allows for a more focused arc, which is essential when welding thin materials or producing intricate welds.The welding machine should be set for alternating current (AC) because DC (direct current) would cause the electrode to become consumed rapidly. The AC current alternates the flow of electricity between the electrode and the workpiece, ensuring that the tungsten electrode stays intact while the arc is in operation.

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procaine (novocaine) is metabolized primarily by the group of answer choices liver. lungs. plasma. kidneys.

Answers

Answer: plasma

Explanation:

What will be the most likely impact of the decline in frog populations resulting from the fungal infection?​

Answers

Answer: A new species of frogs that feed on both the fungus and the infected species of frogs will evolve.

Explanation:

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